Opened 5 years ago

Closed 5 years ago

#3212 closed defect (can't reproduce)

Drawing silhouette: glFramebufferTexture2D: invalid operation

Reported by: chimerax-bug-report@… Owned by: Tom Goddard
Priority: normal Milestone:
Component: Graphics Version: 0.92
Keywords: Cc:
Blocked By: Blocking:
Notify when closed: Platform: all
Project: ChimeraX

Description

The following bug report has been submitted:
Platform:        Linux-4.4.0-178-generic-x86_64-with-debian-stretch-sid
ChimeraX Version: 0.92 (2020-03-05)
Description
(Describe the actions that caused this problem to occur here)

Log:
UCSF ChimeraX version: 0.92 (2020-03-05)  
© 2016-2020 Regents of the University of California. All rights reserved.  
How to cite UCSF ChimeraX  

> open /data/disk3/modeling/gp17-2020/Figure4-20200512-fiber-ET-color-
> change.cxs

opened ChimeraX session  

> select clear

> show #!26 models

> transparency #26 50

> hide #!83 models

> surface dust #50 size 30

> surface dust #26 size 30

> surface dust #34 size 30

> view name J6

> view name J7

> view J6

> open /data/disk3/modeling/nochan-new-trim-gp17-c1-short.mrc

Opened nochan-new-trim-gp17-c1-short.mrc, grid size 160,160,160, pixel 5.4,
shown at level 2.05e-05, step 1, values float32  

> select #1

2 models selected  

> lighting flat

> ui mousemode rightMode "translate selected models"

> ui mousemode rightMode "rotate selected models"

> ui mousemode rightMode "translate selected models"

> ui mousemode rightMode "rotate selected models"

> ui mousemode rightMode "translate selected models"

> hide #!1 models

> show #!1 models

> show #!9 models

> hide #!9 models

> show #!9 models

> ui mousemode rightMode "rotate selected models"

> ui mousemode rightMode "translate selected models"

> ui mousemode rightMode "rotate selected models"

> ui mousemode rightMode "translate selected models"

> fitmap #1 inMap #9

Fit map nochan-new-trim-gp17-c1-short.mrc in map nochan-new-trim.mrc using 934
points  
correlation = 0.9922, correlation about mean = 0.9617, overlap = 34.87  
steps = 60, shift = 9.74, angle = 32 degrees  
  
Position of nochan-new-trim-gp17-c1-short.mrc (#1) relative to nochan-new-
trim.mrc (#9) coordinates:  
Matrix rotation and translation  
-0.50611525 0.86236772 -0.01301046 1.38911285  
-0.86245305 -0.50613234 0.00218729 -1.21911776  
-0.00469876 0.01232793 0.99991297 -1.06579535  
Axis 0.00587907 -0.00481873 -0.99997111  
Axis point 0.34386807 -1.00288964 0.00000000  
Rotation angle (degrees) 120.40887188  
Shift along axis 1.07980585  
  

> show #!17 models

> fitmap #9 inMap #17

Fit map nochan-new-trim.mrc in map nochan-new-fiber.mrc using 14061 points  
correlation = 0.6271, correlation about mean = 0.3689, overlap = 211.1  
steps = 40, shift = 0.0406, angle = 0.0249 degrees  
  
Position of nochan-new-trim.mrc (#9) relative to nochan-new-fiber.mrc (#17)
coordinates:  
Matrix rotation and translation  
0.49952609 0.86629883 0.00000834 -0.00416588  
-0.86629884 0.49952609 -0.00000026 -0.00265282  
-0.00000438 -0.00000709 0.99999999 -0.04873786  
Axis -0.00000395 0.00000734 -1.00000000  
Axis point -0.00437911 0.00227873 0.00000000  
Rotation angle (degrees) 60.03134871  
Shift along axis 0.04873786  
  

> hide #!17 models

> hide #!9 models

> select clear

> show #!17 models

> volume #1 level 0.05692

> volume #1 color #d3d7cfb3

> hide #!17 models

> select clear

> view name test

Unknown command: viewm J7  

> view J7

> view J6

> volume #1 color white

> transparency #6 50

> transparency #1 50

> select clear

> view J6

> lighting soft

> lighting full

> view J7

> save image /data/disk3/Paper-use/Figure4-20200514-6.tif pixelSize 0.1
> supersample 3 transparentBackground true

> transparency #1 70

> volume #1 color #44cccc

> transparency #1 70

> volume #1 color white

> transparency #1 70

> save image /data/disk3/Paper-use/Figure4-20200514-7.tif pixelSize 0.1
> supersample 3 transparentBackground true

> show #!9 models

> hide #!9 models

> show #!17 models

> hide #!17 models

> show #!9 models

> select #9

2 models selected  

> hide #!9 models

> ~select #9

Nothing selected  

> select #9

2 models selected  

> ~select #9

Nothing selected  

> view J6

> save image /data/disk3/Paper-use/Figure4-20200514-8.tif pixelSize 0.1
> supersample 3 transparentBackground true

> show #!47 models

> hide #!47 models

> show #!46 models

> hide #!46 models

> show #!45 models

> hide #!45 models

> show #!44 models

> hide #!44 models

> hide #!97 models

> show #!97 models

> select #97

2 models selected  

> show #!103 models

> hide #!103 models

> show #!102 models

> hide #!102 models

> show #!100 models

> hide #!100 models

> show #!100 models

> hide #!67 models

> show #!67 models

> hide #!67 models

> hide #!100 models

> select clear

> save image /data/disk3/Paper-use/Figure4-20200514-9.tif pixelSize 0.1
> supersample 3 transparentBackground true

> show #!100 models

> show #!9 models

> hide #!9 models

> show #!7 models

> hide #!7 models

> show #!7 models

> hide #!7 models

> hide #!97 models

> show #!97 models

> view J7

> select #97

2 models selected  

> ui mousemode rightMode "rotate selected models"

> ui mousemode rightMode "translate selected models"

> ui mousemode rightMode "rotate selected models"

> view J7

> open /data/disk3/modeling/gp17/fiber-6-fromtail-clean-link-C1.mrc

Opened fiber-6-fromtail-clean-link-C1.mrc, grid size 400,400,400, pixel 1.05,
shown at level 9.12e-07, step 2, values float32  

> select #94

2 models selected  

> ui mousemode rightMode "translate selected models"

> ui mousemode rightMode "rotate selected models"

> ui mousemode rightMode "translate selected models"

> ui mousemode rightMode "rotate selected models"

> ui mousemode rightMode "rotate selected models"

> volume #94 level 0.007616

> volume #94 color #44cccc

> volume #94 color #2cd5cc

> select clear

> view J7

> view J6

> open /data/disk3/modeling/gp17-2020/gp17-shaft-full-right.pdb

Chain information for gp17-shaft-full-right.pdb #107  
---  
Chain | Description  
A B C | No description available  
  

> hide #!68 models

> show #!68 models

> hide #!69 models

> show #!69 models

> select #107

2886 atoms, 2922 bonds, 1 model selected  

> ui mousemode rightMode "translate selected models"

> ui mousemode rightMode "rotate selected models"

> ui mousemode rightMode "translate selected models"

> ui mousemode rightMode "rotate selected models"

> ui mousemode rightMode "translate selected models"

> ui mousemode rightMode "rotate selected models"

> ui mousemode rightMode "translate selected models"

> ui mousemode rightMode "rotate selected models"

> ui mousemode rightMode "translate selected models"

> fitmap #107 inMap #100

Fit molecule gp17-shaft-full-right.pdb (#107) to map shaft-C-4.6-c1-cut.mrc
(#100) using 2886 atoms  
average map value = 0.01205, steps = 268  
shifted from previous position = 3.91  
rotated from previous position = 24.7 degrees  
atoms outside contour = 1208, contour level = 0.011  
  
Position of gp17-shaft-full-right.pdb (#107) relative to
shaft-C-4.6-c1-cut.mrc (#100) coordinates:  
Matrix rotation and translation  
-0.36507994 0.18551431 -0.91230543 412.71672054  
-0.29347167 0.90705012 0.30188507 184.92295630  
0.88351071 0.37794795 -0.27670259 75.21949736  
Axis 0.04089066 -0.96541320 -0.25749818  
Axis point 200.19679794 0.00000000 160.94842252  
Rotation angle (degrees) 111.55327465  
Shift along axis -181.01968607  
  

> select clear

> select #107

2886 atoms, 2922 bonds, 1 model selected  

> ui mousemode rightMode "rotate selected models"

> fitmap #107 inMap #100

Fit molecule gp17-shaft-full-right.pdb (#107) to map shaft-C-4.6-c1-cut.mrc
(#100) using 2886 atoms  
average map value = 0.01205, steps = 92  
shifted from previous position = 0.348  
rotated from previous position = 5.15 degrees  
atoms outside contour = 1199, contour level = 0.011  
  
Position of gp17-shaft-full-right.pdb (#107) relative to
shaft-C-4.6-c1-cut.mrc (#100) coordinates:  
Matrix rotation and translation  
-0.35647978 0.17524692 -0.91772044 414.65031055  
-0.27522767 0.91896846 0.28239480 182.61820863  
0.89284492 0.35325007 -0.27936086 79.40951598  
Axis 0.03794919 -0.96971612 -0.24126854  
Axis point 198.19244484 -0.00000000 166.34318212  
Rotation angle (degrees) 111.00418063  
Shift along axis -180.51119438  
  

> select clear

> view J6

> hide #!97 models

> hide #!95 models

> hide #!34 models

> show #!34 models

> hide #!69 models

> show #!69 models

> hide #!68 models

> open
> /data/disk3/modeling/gp11-gp12-gp17-new-2020/gp17_cycle00_real_space_refined.pdb

Chain information for gp17_cycle00_real_space_refined.pdb #108  
---  
Chain | Description  
C E F | No description available  
  

> hide #108 models

> show #108 models

> hide #!69 models

> show #!69 models

> matchmaker #108 to #69

Parameters  
---  
Chain pairing | bb  
Alignment algorithm | Needleman-Wunsch  
Similarity matrix | BLOSUM-62  
SS fraction | 0.3  
Gap open (HH/SS/other) | 18/18/6  
Gap extend | 1  
SS matrix |  |  | H | S | O  
---|---|---|---  
H | 6 | -9 | -6  
S |  | 6 | -6  
O |  |  | 4  
Iteration cutoff | 2  
  
Matchmaker gp17-refined-good.pdb, chain B (#69) with
gp17_cycle00_real_space_refined.pdb, chain C (#108), sequence alignment score
= 526  
RMSD between 111 pruned atom pairs is 0.952 angstroms; (across all 116 pairs:
1.063)  
  

> view J6

> hide #!69 models

> select #108

2787 atoms, 2829 bonds, 1 model selected  

> show #!69 models

> hide #!69 models

> show sel surfaces

> color #108:96-120 #44CCCC

> color #108:74-95 pink

> select clear

> show #!17 models

> hide #!17 models

> show #!17 models

> fitmap #108 inMap #17

Fit molecule gp17_cycle00_real_space_refined.pdb (#108) to map nochan-new-
fiber.mrc (#17) using 2787 atoms  
average map value = 0.1912, steps = 64  
shifted from previous position = 6.84  
rotated from previous position = 5.01 degrees  
atoms outside contour = 340, contour level = 0.067399  
  
Position of gp17_cycle00_real_space_refined.pdb (#108) relative to nochan-new-
fiber.mrc (#17) coordinates:  
Matrix rotation and translation  
-0.71944904 -0.69452681 -0.00505747 305.11209213  
-0.69136290 0.71682988 -0.09040054 19.41620194  
0.06641095 -0.06154203 -0.99589265 500.58704096  
Axis 0.37410626 -0.92647841 0.04101527  
Axis point 151.31406158 0.00000000 253.44548729  
Rotation angle (degrees) 177.78955720  
Shift along axis 116.68736621  
  

> hide #107 models

> hide #!100 models

> transparency #17 30

> save image /data/disk3/Paper-use/Figure4-20200514-10.tif pixelSize 0.1
> supersample 3 transparentBackground true

> hide #!34 models

> show #!34 models

> hide #!17 models

> view name J8

> fitmap #94 inMap #1

Fit map fiber-6-fromtail-clean-link-C1.mrc in map nochan-new-trim-
gp17-c1-short.mrc using 664 points  
correlation = 0.9588, correlation about mean = 0.08613, overlap = 1.645  
steps = 252, shift = 28.1, angle = 111 degrees  
  
Position of fiber-6-fromtail-clean-link-C1.mrc (#94) relative to nochan-new-
trim-gp17-c1-short.mrc (#1) coordinates:  
Matrix rotation and translation  
-0.21654387 0.97263279 0.08422717 -314.89959974  
0.73244650 0.10481502 0.67270792 -232.88374737  
0.64546950 0.20736267 -0.73509853 252.05197211  
Axis -0.60622434 -0.73115341 -0.31290053  
Axis point -107.35239874 0.00000000 156.24074184  
Rotation angle (degrees) 157.43035619  
Shift along axis 282.30635056  
  

> view J8

> select #94

2 models selected  

> ui mousemode rightMode "rotate selected models"

> ui mousemode rightMode "translate selected models"

> view J7

> view J6

> ui mousemode rightMode "rotate selected models"

> ui mousemode rightMode "translate selected models"

> ui mousemode rightMode "rotate selected models"

> view J6

> ui mousemode rightMode "translate selected models"

> view J6

> hide #!94 models

> show #!94 models

> view J7

> view J7

> hide #!108 models

> show #!108 models

> hide #!108 models

> show #!108 models

> fitmap #108 inMap #94

Fit molecule gp17_cycle00_real_space_refined.pdb (#108) to map
fiber-6-fromtail-clean-link-C1.mrc (#94) using 2787 atoms  
average map value = 0.001075, steps = 224  
shifted from previous position = 13.1  
rotated from previous position = 9.66 degrees  
atoms outside contour = 2614, contour level = 0.0076162  
  
Position of gp17_cycle00_real_space_refined.pdb (#108) relative to
fiber-6-fromtail-clean-link-C1.mrc (#94) coordinates:  
Matrix rotation and translation  
0.49194187 0.08724896 0.86624524 -186.27023198  
-0.42673686 0.89141517 0.15256036 200.67904219  
-0.75887341 -0.44470960 0.47575679 270.80705413  
Axis -0.33069969 0.89980457 -0.28458647  
Axis point 223.29305107 0.00000000 282.96425381  
Rotation angle (degrees) 64.56055610  
Shift along axis 165.10340288  
  

> view J8

> view J7

> view J6

> volume #1 color #2cd5cc

> volume #1 color #ffffff4c

> view J8

> view J8

> view J7

> view J6

> view J8

> view J5

> view J6

> show #!96 models

> hide #!96 models

> show #!92 models

> hide #!92 models

> show #!9 models

> hide #!9 models

> show #!9 models

> hide #!9 models

> show #!9 models

> hide #!9 models

> show #!9 models

> hide #!9 models

> view J6

> view J8

> view J7

> show #!17 models

> hide #!17 models

> show #!54 models

> hide #!54 models

> show #!55 models

> volume #55 level 0.0008263

> volume #55 step 1

> volume #55 level 0.0008976

> volume #55 color #ffffff4c

> view J6

> view J1

> view J4

> view J6

> view J7

> show #!81 models

> hide #!81 models

> show #!9 models

> hide #!9 models

> show #!17 models

> hide #!17 models

> show #!17 models

> select #17

2 models selected  

> hide #!17 models

> hide #17.1 models

> show #17.1 models

> hide #!17 models

> show #!17 models

> save session /data/disk3/modeling/gp17-2020/Figure4-20200514-fiber-ET-color-
> change.cxs

> hide #!1 models

> show #!1 models

> hide #!34 models

> hide #!26 models

> hide #!108 models

> view J7

> open /data/disk3/modeling/gp17-2020/free-fiber-C1.mrc

Opened free-fiber-C1.mrc, grid size 360,360,360, pixel 1.05, shown at level
9.98e-07, step 2, values float32  

> hide #!67.2 models

> hide #!67.1 models

> show #!67.2 models

> hide #!67.2 models

> hide #!67 models

> hide #!109 models

> show #!109 models

> select #109

2 models selected  

> ui mousemode rightMode "rotate selected models"

> ui mousemode rightMode "translate selected models"

> ui mousemode rightMode "rotate selected models"

> ui mousemode rightMode "translate selected models"

> volume #109 level 0.001403

> volume #109 step 1

> ui mousemode rightMode "rotate selected models"

> ui mousemode rightMode "translate selected models"

> show #!92 models

> hide #!92 models

> show #!91 models

> hide #!91 models

> show #!91 models

> fitmap #109 inMap #91

Fit map free-fiber-C1.mrc in map shaft-C-4.6-c6.mrc using 23884 points  
correlation = 0, correlation about mean = 0, overlap = 0  
steps = 84, shift = 15.4, angle = 19.7 degrees  
  
Position of free-fiber-C1.mrc (#109) relative to shaft-C-4.6-c6.mrc (#91)
coordinates:  
Matrix rotation and translation  
0.00139109 0.73296791 0.68026175 154.49950216  
-0.57282837 -0.55700999 0.60133810 450.86289091  
0.81967412 -0.39050974 0.41908996 155.59587546  
Axis -0.60269351 -0.08471353 -0.79346339  
Axis point 160.08955027 216.81081922 0.00000000  
Rotation angle (degrees) 124.62929111  
Shift along axis -254.76966471  
  

> hide #!91 models

> volume #109 level 0.005456

> show #!97 models

> hide #!97 models

> show #!86 models

> hide #!86 models

> show #!89 models

> hide #!89 models

> close #109

> open /data/disk3/modeling/gp17-2020/free-fiber-C1.mrc

Opened free-fiber-C1.mrc, grid size 680,680,680, pixel 1.05, shown at step 1,
values float32  

> toolshed show "Side View"

> open /data/disk3/modeling/gp17-2020/free-fiber-C1.mrc

Opened free-fiber-C1.mrc, grid size 680,680,680, pixel 1.05, shown at step 1,
values float32  

> volume #109 level 0.0001,0 level 9.001e-05,0.8 level 0,1

> volume #110 region 0,0,0,679,679,679 step 4

> volume #110 region 0,0,0,679,679,679 step 4

> volume #110 change image level 0.0001,0 level 9.001e-05,0.8 level 0,1

> volume #110 level 0.002883

> select #110

3 models selected  

> ui mousemode rightMode "rotate selected models"

> volume #110 step 1

> volume #110 level 0.002337

> open /data/disk3/modeling/gp17-2020/free-fiber-C1.mrc

Opened free-fiber-C1.mrc, grid size 680,680,680, pixel 1.05, shown at step 1,
values float32  

> volume #109 region 0,0,0,679,679,679 step 4

> volume #109 region 0,0,0,679,679,679 step 4

> volume #109 step 1

> volume #109 change image level -2.414e-22,0 level 4.588e-19,0.8 level
> 4.59e-18,1

> volume #109 level 0.002555

> select #109

3 models selected  

> ui mousemode rightMode "rotate selected models"

> volume #109 level 0.0006767

> ui mousemode rightMode "translate selected models"

> lighting flat

> ui mousemode rightMode "translate selected models"

> ui mousemode rightMode "rotate selected models"

> ui mousemode rightMode "translate selected models"

> ui mousemode rightMode "rotate selected models"

> volume #109 level 0.001278

> hide #!17 models

> show #!17 models

> hide #!17 models

> show #!27 models

> ui mousemode rightMode "translate selected models"

> ui mousemode rightMode "rotate selected models"

> ui mousemode rightMode "translate selected models"

> hide #!27 models

> show #!10 models

> fitmap #109 inMap #10

Fit map free-fiber-C1.mrc in map fiber-c6-flip-trim.mrc using 94402 points  
correlation = 0.9888, correlation about mean = 0.9274, overlap = 2.64  
steps = 176, shift = 13.5, angle = 17.4 degrees  
  
Position of free-fiber-C1.mrc (#109) relative to fiber-c6-flip-trim.mrc (#10)
coordinates:  
Matrix rotation and translation  
0.99999884 -0.00018523 0.00153708 -0.36970164  
0.00018436 0.99999985 0.00055040 -0.27833222  
-0.00153718 -0.00055011 0.99999868 0.83609747  
Axis -0.33489358 0.93551978 0.11246791  
Axis point 546.81189732 0.00000000 250.21720667  
Rotation angle (degrees) 0.09414132  
Shift along axis -0.04254046  
  

> hide #!10 models

> select clear

> show #!108 models

> hide #!108 models

> show #!108 models

> show #!1 models

> show #!62 models

> hide #!62 models

> show #!69 models

> hide #!69 models

> show #!69 models

> hide #!108 models

> hide #!109 models

> show #!109 models

> hide #!109 models

> hide #!1 models

> show #!1 models

> show #!108 models

> show #!109 models

> hide #!108 models

> hide #!69 models

> show #!69 models

> save session /data/disk3/modeling/gp17-2020/Figure4-20200514-fiber-ET-color-
> change.cxs

> hide #!69 models

> show #!69 models

> hide #!109 models

> show #!108 models

> hide #!108 models

> hide #!1 models

> select #69

2787 atoms, 2829 bonds, 1 model selected  

> show sel surfaces

> hide #!69 models

> show #!69 models

> hide #69.1 models

> show #69.1 models

> hide #69.3 models

> show #69.3 models

> show #!1 models

> show #!108 models

> show #!109 models

> matchmaker #69 to #108

Parameters  
---  
Chain pairing | bb  
Alignment algorithm | Needleman-Wunsch  
Similarity matrix | BLOSUM-62  
SS fraction | 0.3  
Gap open (HH/SS/other) | 18/18/6  
Gap extend | 1  
SS matrix |  |  | H | S | O  
---|---|---|---  
H | 6 | -9 | -6  
S |  | 6 | -6  
O |  |  | 4  
Iteration cutoff | 2  
  
Matchmaker gp17_cycle00_real_space_refined.pdb, chain C (#108) with
gp17-refined-good.pdb, chain B (#69), sequence alignment score = 526  
RMSD between 111 pruned atom pairs is 0.952 angstroms; (across all 116 pairs:
1.063)  
  

> hide #!108 models

> show #!108 models

> hide #!108 models

> hide #!109 models

> show #!109 models

> show #!108 models

> hide #!108 models

> hide #!1 models

> hide #!109 models

> show #!109 models

> fitmap #69 inMap #109

Fit molecule gp17-refined-good.pdb (#69) to map free-fiber-C1.mrc (#109) using
2787 atoms  
average map value = 0.004, steps = 96  
shifted from previous position = 5.41  
rotated from previous position = 7.91 degrees  
atoms outside contour = 627, contour level = 0.0012778  
  
Position of gp17-refined-good.pdb (#69) relative to free-fiber-C1.mrc (#109)
coordinates:  
Matrix rotation and translation  
0.82760955 -0.26266447 -0.49605415 437.65948765  
0.54514984 0.58663312 0.59889331 143.11501459  
0.13369382 -0.76607370 0.62869470 268.99035291  
Axis -0.79984436 -0.36902017 0.47336361  
Axis point 0.00000000 415.81491740 217.81756995  
Rotation angle (degrees) 58.56917929  
Shift along axis -275.54155529  
  

> lighting flat

> ui mousemode rightMode "rotate selected models"

> fitmap #69 inMap #109

Fit molecule gp17-refined-good.pdb (#69) to map free-fiber-C1.mrc (#109) using
2787 atoms  
average map value = 0.004188, steps = 116  
shifted from previous position = 3.21  
rotated from previous position = 21 degrees  
atoms outside contour = 478, contour level = 0.0012778  
  
Position of gp17-refined-good.pdb (#69) relative to free-fiber-C1.mrc (#109)
coordinates:  
Matrix rotation and translation  
-0.20593789 -0.73229163 0.64910589 494.21981877  
0.95668599 -0.29014663 -0.02380817 365.85024705  
0.20577043 0.61608753 0.76032537 -46.20041028  
Axis 0.34407678 0.23838485 0.90817610  
Axis point 138.14702317 346.23057688 -0.00000000  
Rotation angle (degrees) 111.58490511  
Shift along axis 215.30461138  
  

> matchmaker #108 to #69

Parameters  
---  
Chain pairing | bb  
Alignment algorithm | Needleman-Wunsch  
Similarity matrix | BLOSUM-62  
SS fraction | 0.3  
Gap open (HH/SS/other) | 18/18/6  
Gap extend | 1  
SS matrix |  |  | H | S | O  
---|---|---|---  
H | 6 | -9 | -6  
S |  | 6 | -6  
O |  |  | 4  
Iteration cutoff | 2  
  
Matchmaker gp17-refined-good.pdb, chain B (#69) with
gp17_cycle00_real_space_refined.pdb, chain C (#108), sequence alignment score
= 526  
RMSD between 111 pruned atom pairs is 0.952 angstroms; (across all 116 pairs:
1.063)  
  

> show #!108 models

> fitmap #108 inMap #1

Fit molecule gp17_cycle00_real_space_refined.pdb (#108) to map nochan-new-
trim-gp17-c1-short.mrc (#1) using 2787 atoms  
average map value = 0.1813, steps = 52  
shifted from previous position = 5.27  
rotated from previous position = 6.65 degrees  
atoms outside contour = 435, contour level = 0.056916  
  
Position of gp17_cycle00_real_space_refined.pdb (#108) relative to nochan-new-
trim-gp17-c1-short.mrc (#1) coordinates:  
Matrix rotation and translation  
-0.16522467 0.80677202 0.56729158 -289.82076006  
-0.01977205 0.57237196 -0.81975571 159.48415871  
-0.98605775 -0.14666038 -0.07861843 489.22967694  
Axis 0.35728586 0.82453366 -0.43873802  
Axis point -5.28012506 0.00000000 382.45879374  
Rotation angle (degrees) 109.61727403  
Shift along axis -186.69246237  
  

> hide #!108 models

> show #!108 models

> hide #!109 models

> show #!109 models

> hide #!108 models

> lighting soft

> view name J10

> select clear

> volume #109 level 0.002236

> volume #109 level 0.001672

> volume #109 level 0.001278

> volume #109 level 0.00124

> view J10

> view name J11

> view J10

> save image /data/disk3/Paper-use/Figure4-20200514-A1.tif pixelSize 0.1
> supersample 3 transparentBackground true

> hide #!109 models

> show #!108 models

> hide #!69 models

> show #!1 models

> hide #69.3 models

> hide #!108 models

> show #!69 models

> volume #94 step 1

> volume #1 level 0.0554

> volume #1 color #ffffff4c

> show #69.3 models

> save image /data/disk3/Paper-use/Figure4-20200514-A2.tif pixelSize 0.1
> supersample 3 transparentBackground true

> show #!101 models

> hide #!101 models

> show #!101 models

> hide #!101 models

> show #!104 models

> hide #!104 models

> show #!103 models

> hide #!103 models

> show #!102 models

> hide #!102 models

> show #!101 models

> hide #!101 models

> show #!105 models

> show #!100 models

> hide #!105 models

> show #!101 models

> hide #!101 models

> show #!101 models

> hide #!101 models

> show #!101 models

> show #!102 models

> show #!103 models

> show #!104 models

> show #!105 models

> hide #!100 models

> show #!100 models

> hide #!100 models

> hide #!101 models

> hide #!102 models

> hide #!103 models

> hide #!104 models

> hide #!105 models

> hide #!90 models

> show #!90 models

> show #!108 models

> hide #!108 models

> show #!108 models

> show #!109 models

> hide #!108 models

> select #108

2787 atoms, 2829 bonds, 1 model selected  

> show #!108 models

> hide #!108 models

> save session /data/disk3/modeling/gp17-2020/Figure4-20200514-fiber-ET-color-
> change.cxs

> show #!108 models

> hide #!108 models

> show #!108 models

> hide #!109 models

> hide #!69 models

> show #!69 models

> hide #!69 models

> show #!69 models

> hide #!69 models

> show #!69 models

> hide #!69 models

> show #!69 models

> hide #!108 models

> show #!108 models

> show #!109 models

> hide #!69 models

> show #!69 models

> hide #!108 models

> show #!108 models

> hide #!108 models

> hide #!109 models

> show #!109 models

> hide #!109 models

> show #!108 models

> hide #!108 models

> show #!108 models

> hide #!108 models

> show #!108 models

> hide #!108 models

> show #!109 models

> show #!97 models

> hide #!97 models

> show #!97 models

> fitmap #97 inMap #92

Fit map fiber-6-fromtail-clean-link-C1.mrc in map fiber-5.4-seg-c6.mrc using
5406 points  
correlation = 0.8479, correlation about mean = 0.2135, overlap = 0.4846  
steps = 48, shift = 0.00923, angle = 0.0319 degrees  
  
Position of fiber-6-fromtail-clean-link-C1.mrc (#97) relative to
fiber-5.4-seg-c6.mrc (#92) coordinates:  
Matrix rotation and translation  
0.21628094 0.60261668 -0.76816385 225.35643588  
-0.96262041 0.00022601 -0.27085400 569.15151662  
-0.16304752 0.79803076 0.58014000 -79.80942989  
Axis 0.53722655 -0.30413434 -0.78669558  
Axis point 413.95329284 173.98181280 0.00000000  
Rotation angle (degrees) 95.83572035  
Shift along axis 10.75466726  
  

> hide #!109 models

> show #!109 models

> hide #!1 models

> show #!95 models

> fitmap #95 inMap #92

Fit map shaft-C-4.6.mrc in map fiber-5.4-seg-c6.mrc using 70731 points  
correlation = 0.9157, correlation about mean = 0.5212, overlap = 14.35  
steps = 44, shift = 0.0392, angle = 0.0383 degrees  
  
Position of shaft-C-4.6.mrc (#95) relative to fiber-5.4-seg-c6.mrc (#92)
coordinates:  
Matrix rotation and translation  
-0.19604015 0.03454125 -0.97998730 437.19127359  
0.00062180 0.99938357 0.03510051 160.37128780  
0.98059565 0.00627176 -0.19594080 122.15269742  
Axis -0.01470039 -0.99974234 -0.01729624  
Axis point 168.53987427 0.00000000 237.88916305  
Rotation angle (degrees) 101.32059982  
Shift along axis -168.86962900  
  

> hide #!95 models

> show #!70 models

> hide #!70 models

> show #!70 models

> select #70

2787 atoms, 2829 bonds, 1 model selected  

> fitmap #70 inMap #92

Fit molecule gp17_real_space_refined-coot-4.pdb (#70) to map
fiber-5.4-seg-c6.mrc (#92) using 2787 atoms  
average map value = 0.006346, steps = 88  
shifted from previous position = 5.37  
rotated from previous position = 1.69 degrees  
atoms outside contour = 1268, contour level = 0.002996  
  
Position of gp17_real_space_refined-coot-4.pdb (#70) relative to
fiber-5.4-seg-c6.mrc (#92) coordinates:  
Matrix rotation and translation  
-0.86091270 0.50788871 0.02963773 424.84172141  
-0.50828642 -0.86115651 -0.00737465 643.84806886  
0.02177722 -0.02141339 0.99953351 27.36641270  
Axis -0.01381354 0.00773442 -0.99987467  
Axis point 300.16227920 264.08212689 0.00000000  
Rotation angle (degrees) 149.45923624  
Shift along axis -28.25176151  
  

> hide #!70 models

> show #!70 models

> hide #!70 models

> show #!70 models

> hide #!70 models

> close #70

> show #!71 models

> hide #!71 models

> show #!71 models

> fitmap #71 inMap #92

Fit molecule gp17_real_space_refined-coot-4-c6.pdb (#71) to map
fiber-5.4-seg-c6.mrc (#92) using 16722 atoms  
average map value = 0.006028, steps = 76  
shifted from previous position = 2.41  
rotated from previous position = 1.36 degrees  
atoms outside contour = 7984, contour level = 0.002996  
  
Position of gp17_real_space_refined-coot-4-c6.pdb (#71) relative to
fiber-5.4-seg-c6.mrc (#92) coordinates:  
Matrix rotation and translation  
-0.84616000 0.53290997 -0.00449530 423.41401875  
-0.53292617 -0.84609842 0.01035045 644.99482400  
0.00171239 0.01115380 0.99993632 28.11846062  
Axis 0.00075372 -0.00582415 -0.99998276  
Axis point 304.78730585 261.29804255 0.00000000  
Rotation angle (degrees) 147.79654753  
Shift along axis -31.55538840  
  

> hide #!71 models

> save image /data/disk3/Paper-use/Figure4-20200514-A1.tif pixelSize 0.1
> supersample 3 transparentBackground true

> select #97

2 models selected  

> ui mousemode rightMode "translate selected models"

> fitmap #97 inMap #92

Fit map fiber-6-fromtail-clean-link-C1.mrc in map fiber-5.4-seg-c6.mrc using
5406 points  
correlation = 0.8479, correlation about mean = 0.2135, overlap = 0.4846  
steps = 88, shift = 7.04, angle = 12 degrees  
  
Position of fiber-6-fromtail-clean-link-C1.mrc (#97) relative to
fiber-5.4-seg-c6.mrc (#92) coordinates:  
Matrix rotation and translation  
0.21639546 0.60305635 -0.76778646 225.14300036  
-0.96256676 0.00031634 -0.27104450 569.15919342  
-0.16321223 0.79769853 0.58055046 -79.77927727  
Axis 0.53713836 -0.30385229 -0.78686477  
Axis point 413.90369154 174.07624366 0.00000000  
Rotation angle (degrees) 95.81800153  
Shift along axis 10.76812333  
  

> select clear

> show #107 models

> hide #107 models

> show #107 models

> hide #107 models

> show #!89 models

> hide #!89 models

> show #!68 models

> fitmap #68 inMap #92

Fit molecule gp17-shaft-full-right.pdb (#68) to map fiber-5.4-seg-c6.mrc (#92)
using 2886 atoms  
average map value = 0.009752, steps = 68  
shifted from previous position = 1.84  
rotated from previous position = 0.201 degrees  
atoms outside contour = 741, contour level = 0.002996  
  
Position of gp17-shaft-full-right.pdb (#68) relative to fiber-5.4-seg-c6.mrc
(#92) coordinates:  
Matrix rotation and translation  
-0.19306896 0.01797353 -0.98102056 440.92451515  
0.01824718 0.99972506 0.01472510 160.82443250  
0.98101550 -0.01505790 -0.19334385 127.73475631  
Axis -0.01517789 -0.99988480 0.00013946  
Axis point 166.71616013 0.00000000 244.11174499  
Rotation angle (degrees) 101.14799363  
Shift along axis -167.48039563  
  

> fitmap #68 inMap #109

Fit molecule gp17-shaft-full-right.pdb (#68) to map free-fiber-C1.mrc (#109)
using 2886 atoms  
average map value = 0.004709, steps = 308  
shifted from previous position = 42.3  
rotated from previous position = 9.63 degrees  
atoms outside contour = 503, contour level = 0.0012402  
  
Position of gp17-shaft-full-right.pdb (#68) relative to free-fiber-C1.mrc
(#109) coordinates:  
Matrix rotation and translation  
-0.03199957 0.87514369 0.48280375 146.36188321  
0.08918726 -0.47862394 0.87347851 357.30940126  
0.99550069 0.07101089 -0.06273592 54.34846318  
Axis -0.64991733 -0.41523249 -0.63654493  
Axis point 0.00000000 99.29127264 -108.78926909  
Rotation angle (degrees) 141.87630089  
Shift along axis -278.08483340  
  

> show #!108 models

> hide #!108 models

> show #!1 models

> show #!89 models

> hide #!89 models

> show #107 models

> fitmap #107 inMap #1

Fit molecule gp17-shaft-full-right.pdb (#107) to map nochan-new-trim-
gp17-c1-short.mrc (#1) using 2886 atoms  
average map value = 0.1817, steps = 256  
shifted from previous position = 57.3  
rotated from previous position = 26.9 degrees  
atoms outside contour = 429, contour level = 0.055398  
  
Position of gp17-shaft-full-right.pdb (#107) relative to nochan-new-trim-
gp17-c1-short.mrc (#1) coordinates:  
Matrix rotation and translation  
-0.83887173 -0.17162536 -0.51656472 190.71825093  
-0.52998756 0.04111938 0.84700797 -33.05706746  
-0.12412722 0.98430385 -0.12545319 86.90437586  
Axis 0.25013343 -0.71496496 -0.65288466  
Axis point 80.14083924 0.00000000 76.84865895  
Rotation angle (degrees) 164.07104231  
Shift along axis 14.60112165  
  

> show #!102 models

> show #!101 models

> show #!100 models

> hide #!100 models

> hide #!101 models

> hide #!102 models

> select #107

2886 atoms, 2922 bonds, 1 model selected  

> select clear

Drag select of 114 residues  

> hide sel cartoons

> select #107

2886 atoms, 2922 bonds, 1 model selected  

> ui mousemode rightMode "rotate selected models"

> ui mousemode rightMode "translate selected models"

> ui mousemode rightMode "rotate selected models"

> ui mousemode rightMode "translate selected models"

Drag select of 43 residues, 109 free-fiber-C1.mrc  

> select clear

Drag select of 28 residues  
Drag select of 22 residues  

> hide sel cartoons

Drag select of 19 residues  

> hide sel cartoons

Drag select of 1 residues, 109 free-fiber-C1.mrc  

> select clear

> select clear

> fitmap #107 inMap #1

Fit molecule gp17-shaft-full-right.pdb (#107) to map nochan-new-trim-
gp17-c1-short.mrc (#1) using 2886 atoms  
average map value = 0.1822, steps = 132  
shifted from previous position = 59.2  
rotated from previous position = 11 degrees  
atoms outside contour = 426, contour level = 0.055398  
  
Position of gp17-shaft-full-right.pdb (#107) relative to nochan-new-trim-
gp17-c1-short.mrc (#1) coordinates:  
Matrix rotation and translation  
-0.84225433 -0.13285118 -0.52245416 184.00319139  
-0.53898500 0.18928569 0.82077173 -59.58758927  
-0.01014740 0.97289343 -0.23103147 88.83844089  
Axis 0.22663272 -0.76324071 -0.60506300  
Axis point 70.80722383 0.00000000 88.01233932  
Rotation angle (degrees) 160.39019237  
Shift along axis 33.42796418  
  

> select #107

2886 atoms, 2922 bonds, 1 model selected  

> ui mousemode rightMode "rotate selected models"

> ui mousemode rightMode "translate selected models"

> ui mousemode rightMode "rotate selected models"

> ui mousemode rightMode "translate selected models"

> ui mousemode rightMode "rotate selected models"

> ui mousemode rightMode "translate selected models"

> select clear

> save session /data/disk3/modeling/gp17-2020/Figure4-20200514-fiber-ET-color-
> change.cxs

> hide #107 models

> hide #!109 models

> show #!109 models

> hide #!109 models

> open /data/disk3/modeling/gp17/fiber-6-fromtail-clean-link-C1.mrc

Opened fiber-6-fromtail-clean-link-C1.mrc, grid size 400,400,400, pixel 1.05,
shown at level 9.12e-07, step 2, values float32  

> select #70

2 models selected  

> ui mousemode rightMode "rotate selected models"

> ui mousemode rightMode "translate selected models"

> volume #70 level 0.007414

> ui mousemode rightMode "rotate selected models"

> ui mousemode rightMode "translate selected models"

> hide #!97 models

> ui mousemode rightMode "rotate selected models"

> ui mousemode rightMode "translate selected models"

> volume #70 step 1

> fitmap #70 inMap #1

Fit map fiber-6-fromtail-clean-link-C1.mrc in map nochan-new-trim-
gp17-c1-short.mrc using 5599 points  
correlation = 0.9579, correlation about mean = 0.0551, overlap = 13.65  
steps = 96, shift = 11.7, angle = 55.2 degrees  
  
Position of fiber-6-fromtail-clean-link-C1.mrc (#70) relative to nochan-new-
trim-gp17-c1-short.mrc (#1) coordinates:  
Matrix rotation and translation  
0.36091389 -0.86661750 -0.34455069 164.59863348  
0.78075595 0.07871174 0.61985850 -219.73489364  
-0.51006006 -0.49272557 0.70502497 328.08228260  
Axis -0.55775276 0.08297198 0.82584957  
Axis point 344.81018145 39.21328111 0.00000000  
Rotation angle (degrees) 85.85244404  
Shift along axis 160.90943096  
  

> ui mousemode rightMode "rotate selected models"

> ui mousemode rightMode "translate selected models"

> ui mousemode rightMode "rotate selected models"

> ui mousemode rightMode "translate selected models"

> show #107 models

> select #107

2886 atoms, 2922 bonds, 1 model selected  

> ui mousemode rightMode "rotate selected models"

> ui mousemode rightMode "translate selected models"

> ui mousemode rightMode "rotate selected models"

> ui mousemode rightMode "translate selected models"

> ui mousemode rightMode "rotate selected models"

> ui mousemode rightMode "translate selected models"

> select #70

2 models selected  

> ui mousemode rightMode "rotate selected models"

> ui mousemode rightMode "translate selected models"

> save session /data/disk3/modeling/gp17-2020/Figure4-20200514-fiber-ET-color-
> change.cxs

> select clear

> volume #70 color #2cd5cc

> volume #70 color #4096bf

> volume #70 color #2cd5cc

> volume #70 color #40bfbf

> volume #70 color #44cccc

> show #!97 models

> hide #107 models

> show #107 models

> show #!109 models

> hide #107 models

> hide #!109 models

> show #!109 models

> hide #!1 models

> hide #!70 models

> view name J10

> save image /data/disk3/Paper-use/Figure4-20200514-A1.tif pixelSize 0.1
> supersample 3 transparentBackground true

> show #!1 models

> hide #!109 models

> show #!109 models

> hide #!1 models

> view J10

> lighting full

> lighting full

> save image /data/disk3/Paper-use/Figure4-20200514-A1.tif pixelSize 0.1
> supersample 3 transparentBackground true

> view name J10

> save image /data/disk3/Paper-use/Figure4-20200514-A1.tif pixelSize 0.1
> supersample 3 transparentBackground true

> show #!108 models

> hide #!108 models

> show #!108 models

> hide #!108 models

> show #!1 models

> hide #!1 models

> show #!1 models

> hide #!1 models

> show #!1 models

> hide #!1 models

> show #!1 models

> hide #!1 models

> show #!1 models

> hide #!109 models

Drag select of 16 residues  

> hide sel cartoons

> show #!109 models

> hide #!109 models

> show #!70 models

> show #107 models

> hide #!1 models

Drag select of 39 residues  

> hide sel cartoons

Drag select of 2 residues  

> hide sel cartoons

> show #!1 models

Drag select of 2 residues  
Drag select of 5 residues  

> hide sel cartoons

> select #68/B:260

9 atoms, 8 bonds, 1 model selected  

> hide sel cartoons

> show #!11 models

> hide #!11 models

> show #!109 models

> view J10

> hide #!109 models

> show #!109 models

> hide #!1 models

> hide #107 models

> hide #!70 models

> view J11

> view J10

> lighting flat

> view J10

> select clear

> save session /data/disk3/modeling/gp17-2020/Figure4-20200514-fiber-ET-color-
> change.cxs

> lighting soft

> hide #!109 models

> show #!109 models

> transparency #109 70

> transparency #109 80

> transparency #109 90

> lighting soft

> lighting full

> view J10

> save image /data/disk3/Paper-use/Figure4-20200514-A1.tif pixelSize 0.1
> supersample 3 transparentBackground true

> hide #!109 models

> show #!108 models

> hide #!97 models

> show #!94 models

> hide #!69 models

> hide #!108 models

> show #!108 models

> show #107 models

> hide #107 models

> show #107 models

> view name J11

> fitmap #107 inMap #1

Fit molecule gp17-shaft-full-right.pdb (#107) to map nochan-new-trim-
gp17-c1-short.mrc (#1) using 2886 atoms  
average map value = 0.1824, steps = 144  
shifted from previous position = 72.1  
rotated from previous position = 13.9 degrees  
atoms outside contour = 417, contour level = 0.055398  
  
Position of gp17-shaft-full-right.pdb (#107) relative to nochan-new-trim-
gp17-c1-short.mrc (#1) coordinates:  
Matrix rotation and translation  
-0.84220659 -0.08996679 -0.53159588 175.94842368  
-0.52662414 0.34860865 0.77533164 -88.81898455  
0.11556482 0.93294057 -0.34097916 95.82858825  
Axis 0.19789014 -0.81256006 -0.54825691  
Axis point 61.09582678 0.00000000 98.98886229  
Rotation angle (degrees) 156.53284560  
Shift along axis 54.45053220  
  

> view J11

> hide #!68 models

> show #!109 models

> hide #!109 models

> show #!109 models

> show #!1 models

> hide #!109 models

> lighting soft

> transparency #1 90

> save image /data/disk3/Paper-use/Figure4-20200514-A2.tif pixelSize 0.1
> supersample 3 transparentBackground true

> save image /data/disk3/Paper-use/Figure4-20200514-A3.tif pixelSize 0.1
> supersample 3 transparentBackground true

> hide #!108 models

> hide #107 models

> show #!109 models

> hide #!70 models

> view name J12

> view J11

> hide #!109 models

> show #!109 models

> volume #1 level 0.06829

> volume #109 level 0.001653

> volume #109 level 0.002086

> volume #109 color #ffffff4c

> volume #109 color #ffffff19

> volume #109 color #ffffff4c

> volume #109 color #44cccc

> volume #109 color #ffffff4c

> show #!108 models

> hide #!108 models

> show #!69 models

> save image /data/disk3/Paper-use/Figure4-20200514-A3.tif pixelSize 0.1
> supersample 3 transparentBackground true

> hide #!109 models

> show #!108 models

> hide #!69 models

> show #!1 models

> volume #1 level 0.05843

> save image /data/disk3/Paper-use/Figure4-20200514-A4.tif pixelSize 0.1
> supersample 3 transparentBackground true

> show #!70 models

> save image /data/disk3/Paper-use/Figure4-20200514-A5.tif pixelSize 0.1
> supersample 3 transparentBackground true

> hide #!70 models

> hide #!1 models

> hide #!94 models

> hide #!108 models

> show #!109 models

> show #107 models

> hide #107 models

> show #!69 models

> show #107 models

> hide #107 models

> fitmap #94 inMap #109

Fit map fiber-6-fromtail-clean-link-C1.mrc in map free-fiber-C1.mrc using 5271
points  
correlation = 0.9144, correlation about mean = 0.1405, overlap = 0.2745  
steps = 144, shift = 12.2, angle = 18.2 degrees  
  
Position of fiber-6-fromtail-clean-link-C1.mrc (#94) relative to free-
fiber-C1.mrc (#109) coordinates:  
Matrix rotation and translation  
0.86388881 0.19657941 0.46373761 146.33764130  
-0.47729758 0.02541986 0.87837396 313.93790623  
0.16088210 -0.98015832 0.11578682 449.74173981  
Axis -0.92926917 0.15142825 -0.33693959  
Axis point 0.00000000 367.87790094 9.39038982  
Rotation angle (degrees) 89.85402500  
Shift along axis -239.98379087  
  

> view J11

> show #!70 models

> hide #!70 models

> show #!97 models

> save image /data/disk3/Paper-use/Figure4-20200514-A6.tif pixelSize 0.1
> supersample 3 transparentBackground true

> save session /data/disk3/modeling/gp17-2020/Figure4-20200514-fiber-ET-color-
> change.cxs

An error occurred in drawing the scene. Redrawing graphics is now stopped to
avoid a continuous stream of error messages. To restart graphics use the
command "graphics restart" after changing the settings that caused the error.  
  
Traceback (most recent call last):  
File "/home/cwang/software/chimerax-2020.03.05/lib/python3.7/site-
packages/chimerax/core/updateloop.py", line 73, in draw_new_frame  
view.draw(check_for_changes = False)  
File "/home/cwang/software/chimerax-2020.03.05/lib/python3.7/site-
packages/chimerax/core/graphics/view.py", line 166, in draw  
self._draw_scene(camera, drawings)  
File "/home/cwang/software/chimerax-2020.03.05/lib/python3.7/site-
packages/chimerax/core/graphics/view.py", line 239, in _draw_scene  
silhouette.draw_silhouette(r)  
File "/home/cwang/software/chimerax-2020.03.05/lib/python3.7/site-
packages/chimerax/core/graphics/opengl.py", line 1668, in draw_silhouette  
fb.attach_depth_texture(None) # Detach depth texture  
File "/home/cwang/software/chimerax-2020.03.05/lib/python3.7/site-
packages/chimerax/core/graphics/opengl.py", line 2038, in attach_depth_texture  
tid, level)  
File "src/errorchecker.pyx", line 58, in
OpenGL_accelerate.errorchecker._ErrorChecker.glCheckError  
OpenGL.error.GLError: GLError(  
err = 1282,  
description = b'invalid operation',  
baseOperation = glFramebufferTexture2D,  
cArguments = (  
GL_FRAMEBUFFER,  
GL_DEPTH_ATTACHMENT,  
GL_TEXTURE_2D,  
0,  
0,  
)  
)  
  




OpenGL version: 3.3.0 NVIDIA 384.130
OpenGL renderer: Quadro K2200/PCIe/SSE2
OpenGL vendor: NVIDIA Corporation

Change History (2)

comment:1 by Eric Pettersen, 5 years ago

Component: UnassignedGraphics
Owner: set to Tom Goddard
Platform: all
Project: ChimeraX
Status: newassigned
Summary: ChimeraX bug report submissionDrawing silhouette: glFramebufferTexture2D: invalid operation
Version: 0.92

comment:2 by Tom Goddard, 5 years ago

Resolution: can't reproduce
Status: assignedclosed

OpenGL InvalidOperation error in glFramebufferTexture2D when drawing silhouettes. OpenGL docs say this can be caused by

GL_INVALID_OPERATION is generated if the default framebuffer object name 0 is bound.

GL_INVALID_OPERATION is generated if texture is neither 0 nor the name of an existing texture object.

GL_INVALID_OPERATION is generated if texture is the name of an existing two-dimensional texture object but textarget is not GL_TEXTURE_2D or if texture is the name of an existing cube map texture object but textarget is GL_TEXTURE_2D.

The texture id is 0 in this call, removing the depth texture so the second two causes for the error can be ruled out. An offscreen framebuffer has been enabled that seems to rule out cause 1, although some incorrect code could have popped that framebuffer.

Possible graphics driver bug. Have seen several reports with Quadro K2200 (might be this same user).

No other bug reports with glFramebufferTexture2D.

Note: See TracTickets for help on using tickets.