Opened 4 years ago

Closed 4 years ago

#4870 closed defect (can't reproduce)

Segger: n is None

Reported by: chimerax-bug-report@… Owned by: Tom Goddard
Priority: normal Milestone:
Component: Volume Data Version:
Keywords: Cc:
Blocked By: Blocking:
Notify when closed: Platform: all
Project: ChimeraX

Description

The following bug report has been submitted:
Platform:        Linux-3.10.0-957.21.2.el7.x86_64-x86_64-with-centos-7.6.1810-Core
ChimeraX Version: 1.0 (2020-06-04 23:15:07 UTC)
Description
(Describe the actions that caused this problem to occur here)

Log:
UCSF ChimeraX version: 1.0 (2020-06-04)  
© 2016-2020 Regents of the University of California. All rights reserved.  

> open /home/priya/Priya_Projects/Msmegmatis_Ribosome_GH/50S+H68/50S+H68.cxs
> format session

Log from Wed Jul 7 20:02:25 2021UCSF ChimeraX version: 1.0 (2020-06-04)  
© 2016-2020 Regents of the University of California. All rights reserved.  

> open /home/priya/Priya_Projects/Msmegmatis_Ribosome_GH/50S+H68/50S+H68.cxs
> format session

Showing cryosparc_P1_J6_map_sharp.seg - 2517 regions, 2517 surfaces  
Log from Wed Jul 7 19:01:13 2021UCSF ChimeraX version: 1.0 (2020-06-04)  
© 2016-2020 Regents of the University of California. All rights reserved.  

> open /home/priya/Priya_Projects/Msmegmatis_Ribosome_GH/50S+H68/50S+H68.cxs
> format session

Log from Wed Jul 7 18:40:22 2021UCSF ChimeraX version: 1.0 (2020-06-04)  
© 2016-2020 Regents of the University of California. All rights reserved.  

> open /home/priya/Priya_Projects/Msmegmatis_Ribosome_GH/50S+H68/50S+H68.cxs
> format session

Showing cryosparc_P1_J6_map_sharp.seg - 2539 regions, 2539 surfaces  
Log from Wed Jul 7 16:44:50 2021UCSF ChimeraX version: 1.0 (2020-06-04)  
© 2016-2020 Regents of the University of California. All rights reserved.  

> open /home/priya/Priya_Projects/Msmegmatis_Ribosome_GH/50S+H68/50S+H68.cxs
> format session

Showing cryosparc_P1_J6_map_sharp.seg - 2596 regions, 2596 surfaces  
Log from Wed Jul 7 14:50:38 2021UCSF ChimeraX version: 1.0 (2020-06-04)  
© 2016-2020 Regents of the University of California. All rights reserved.  
How to cite UCSF ChimeraX  

> open
> /home/priya/Priya_Projects/Msmegmatis_Ribosome_GH/70S_GH_2/P1/J6/cryosparc_P1_J6_mask_fsc.mrc

Opened cryosparc_P1_J6_mask_fsc.mrc, grid size 350,350,350, pixel 1.38, shown
at level 0.0352, step 2, values float32  

> volume #1 step 1

> open
> /home/priya/Priya_Projects/Msmegmatis_Ribosome_GH/70S_GH_2/P1/J6/cryosparc_P1_J6_map_sharp.mrc

Opened cryosparc_P1_J6_map_sharp.mrc, grid size 350,350,350, pixel 1.38, shown
at level 0.0339, step 2, values float32  

> volume #2 step 1

> close #1

> volume #2 level 0.02764

> set bgColor white

> volume #2 color #babdb6

> volume #2 color #888a85

> ui tool show "Segment Map"

Segmenting cryosparc_P1_J6_map_sharp.mrc, density threshold 0.027637  
Showing cryosparc_P1_J6_map_sharp.seg - 0 regions, 0 surfaces  
Showing 124 region surfaces  
7657 watershed regions, grouped to 124 regions  

> open
> /home/priya/Priya_Projects/Msmegmatis_Ribosome_GH/50S+H68/MDFF/50S+H68_coot.pdb

50S+H68_coot.pdb title:  
Structure of the 50S large ribosomal subunit from mycobacterium smegmatis
[more info...]  
  
Chain information for 50S+H68_coot.pdb #3  
---  
Chain | Description  
2 | No description available  
3 | BL37  
A | 23S RRNA  
B | 5S RRNA  
C | UL2  
D | UL3  
E | UL4  
F | UL5  
G | UL6  
H | BL9  
I | UL10  
J | UL11  
K | UL13  
L | UL14  
M | UL15  
N | UL16  
O | BL17  
P | UL18  
Q | BL19  
R | BL20  
S | BL21  
T | UL22  
U | UL23  
V | UL24  
W | 50S ribosomal protein L25  
X | 50S ribosomal protein L27  
Y | No description available  
Z | No description available  
a | No description available  
b | No description available  
c | No description available  
d | No description available  
e | No description available  
f | No description available  
g | No description available  
  
Non-standard residues in 50S+H68_coot.pdb #3  
---  
MG — magnesium ion  
ZN — zinc ion  
  

> show cartoons

> hide surfaces

> hide surfaces

> style sphere

Changed 98153 atom styles  

> style sphere

Changed 98153 atom styles  

> style ball

Changed 98153 atom styles  

> hide atoms

> set bgColor black

> select clear

Grouped 5 regions  
Ungrouped to 3 regions  
Ungrouped to 9 regions  
Ungrouped to 33 regions  
Ungrouped to 93 regions  
Ungrouped to 0 regions  
Grouped 12 regions  
Grouped 4 regions  
Grouped 4 regions  

> hide #!1 models

> show #!1 models

> hide #!2 models

Grouped 2 regions  
Grouped 12 regions  
Grouped 5 regions  
Grouped 4 regions  

> select clear

Ungrouped to 3 regions  
Ungrouped to 8 regions  
Ungrouped to 50 regions  
Ungrouped to 78 regions  
Ungrouped to 0 regions  
Grouped 13 regions  
Grouped 5 regions  
Grouped 8 regions  
Grouped 6 regions  
Grouped 2 regions  
Showing 268 region surfaces  
Grouped 2 regions  

> select clear

Ungrouped to 4 regions  

> select clear

Ungrouped to 130 regions  
Ungrouped to 2 regions  
Ungrouped to 18 regions  
Ungrouped to 46 regions  
Ungrouped to 0 regions  
Grouped 6 regions  
Grouped 8 regions  
Grouped 4 regions  

> select clear

> select clear

Grouped 2 regions  
Grouped 20 regions  
Ungrouped to 20 regions  
Grouped 19 regions  
Showing 366 region surfaces  
Grouped 3 regions  

> select clear

Grouped 4 regions  
Grouped 5 regions  
Grouped 5 regions  
Grouped 5 regions  
Ungrouped to 5 regions  
Grouped 5 regions  
Grouped 2 regions  

> select clear

Ungrouped to 4 regions  
Ungrouped to 19 regions  
Ungrouped to 34 regions  
Ungrouped to 0 regions  
Grouped 2 regions  
Showing 387 region surfaces  
Grouped 3 regions  

> select clear

Ungrouped to 0 regions  
Ungrouped to 3 regions  
Ungrouped to 14 regions  
Ungrouped to 0 regions  
Grouped 3 regions  
Showing 397 region surfaces  
Grouped 2 regions  
Grouped 3 regions  
Grouped 3 regions  

> select clear

Ungrouped to 0 regions  
Ungrouped to 6 regions  
Ungrouped to 11 regions  
Ungrouped to 0 regions  
Showing 405 region surfaces  
Grouped 1 regions  

> select clear

Ungrouped to 2 regions  
Ungrouped to 19 regions  
Ungrouped to 57 regions  
Ungrouped to 0 regions  
Showing 465 region surfaces  
Grouped 7 regions  
Grouped 2 regions  
Grouped 3 regions  
Grouped 2 regions  
Grouped 3 regions  
Grouped 2 regions  

> select clear

Showing 452 region surfaces  
Grouped 2 regions  

> select clear

> select clear

Grouped 2 regions  
Ungrouped to 5 regions  
Ungrouped to 39 regions  
Ungrouped to 93 regions  
Ungrouped to 0 regions  
Grouped 6 regions  
Grouped 2 regions  
Grouped 5 regions  
Grouped 5 regions  
Grouped 3 regions  
Grouped 4 regions  

> select #1

534 models selected  

> ~select #1

Nothing selected  
Ungrouped to 0 regions  
Grouped 4 regions  
Grouped 2 regions  
Grouped 1 regions  
Ungrouped to 1 regions  

> select clear

Showing 529 region surfaces  
Grouped 10 regions  

> select clear

> select clear

Grouped 7 regions  
Grouped 2 regions  

> select clear

Ungrouped to 4 regions  
Ungrouped to 24 regions  
Ungrouped to 61 regions  
Ungrouped to 0 regions  
Showing 581 region surfaces  
Grouped 2 regions  
Grouped 2 regions  

> select clear

Ungrouped to 21 regions  
Ungrouped to 52 regions  
Ungrouped to 0 regions  

> select clear

Showing 636 region surfaces  
Grouped 4 regions  
Grouped 2 regions  
Grouped 2 regions  
Grouped 3 regions  
Grouped 2 regions  
Grouped 2 regions  

> select clear

Ungrouped to 3 regions  
Ungrouped to 20 regions  
Ungrouped to 29 regions  
Ungrouped to 0 regions  
Ungrouped to 0 regions  
Ungrouped to 386 regions  
Grouped 3 regions  
Showing 938 region surfaces  
Grouped 2 regions  
Grouped 2 regions  
Grouped 5 regions  
Grouped 3 regions  
Grouped 7 regions  
Grouped 2 regions  
Showing 923 region surfaces  
Grouped 9 regions  
Ungrouped to 9 regions  
Grouped 7 regions  

> select clear

> select clear

Ungrouped to 7 regions  
Ungrouped to 2 regions  
Ungrouped to 7 regions  
Ungrouped to 3 regions  
Ungrouped to 5 regions  
Ungrouped to 2 regions  
Ungrouped to 2 regions  
Ungrouped to 5 regions  
Ungrouped to 2 regions  
Ungrouped to 3 regions  
Ungrouped to 2 regions  
Ungrouped to 2 regions  
Ungrouped to 4 regions  
Ungrouped to 2 regions  
Ungrouped to 4 regions  
Ungrouped to 7 regions  
Ungrouped to 10 regions  

> select clear

> select clear

> select clear

Drag select of 1901, 6 of 968 triangles, 1624, 5 of 848 triangles, 1 residues  
Grouped 19 regions  
Grouped 8 regions  
Grouped 7 regions  
Grouped 5 regions  
Grouped 2 regions  
Grouped 2 regions  
Grouped 2 regions  
Drag select of 2 residues  
Grouped 8 regions  
Grouped 3 regions  
Grouped 3 regions  

> open /home/priya/Priya_Projects/Msmegmatis_Ribosome_GH/50S+H68/MDFF/L2.pdb

Chain information for L2.pdb #4  
---  
Chain | Description  
C | No description available  
  

> hide #!3 models

Showing 918 region surfaces  

> select clear

> show #!3 models

> hide #4 models

> show #4 models

> hide #4 models

Grouped 5 regions  
Grouped 4 regions  
Grouped 2 regions  
Grouped 12 regions  
Grouped 4 regions  
Showing 896 region surfaces  

> select clear

Grouped 3 regions  
Grouped 2 regions  
Grouped 3 regions  
Grouped 3 regions  

> select clear

> show #4 models

> select #4

2110 atoms, 2153 bonds, 1 model selected  

> show sel atoms

> hide #!3 models

> hide #!1 models

> show #!1 models

> select clear

Showing 889 region surfaces  
Grouped 2 regions  

> select clear

Ungrouped to 3 regions  
Ungrouped to 3 regions  
Ungrouped to 3 regions  
Ungrouped to 4 regions  
Ungrouped to 12 regions  
Ungrouped to 2 regions  
Ungrouped to 4 regions  
Ungrouped to 5 regions  
Ungrouped to 0 regions  

> show #!3 models

> hide #!3 models

> show #!3 models

> hide #!3 models

> show #!3 models

> hide #!3 models

> show #!3 models

> hide #!3 models

> select clear

> hide #4 models

Drag select of 2069, 2155, 1828, 2245, 1486, 1495, 1496, 1376, 1439, 1565,
1700, 2086, 1827, 1441, 2327, 1839, 1906, 2546, 1562, 1917, 1992, 1988, 1687,
2151, 1558, 1899, 1908, 2247, 1768  
Grouped 26 regions  

> show #!3 models

Showing 891 region surfaces  

> show #4 models

Grouped 4 regions  
Ungrouped to 4 regions  
Grouped 2 regions  

> select clear

Showing 890 region surfaces  
Grouped 3 regions  
Grouped 2 regions  
Grouped 2 regions  
Grouped 2 regions  
Grouped 2 regions  
Grouped 2 regions  

> select clear

Ungrouped to 0 regions  
Ungrouped to 0 regions  
Ungrouped to 2106 regions  
Showing 2608 region surfaces  
Grouped 2 regions  

> select clear

Grouped 2 regions  
Grouped 2 regions  
Grouped 2 regions  

> select #4

2110 atoms, 2153 bonds, 1 model selected  

> hide sel atoms

Grouped 4 regions  

> select clear

Showing 2601 region surfaces  
Grouped 2 regions  

> select clear

Ungrouped to 0 regions  
Grouped 2 regions  
Grouped 1 regions  
Ungrouped to 1 regions  
Showing 2599 region surfaces  
Grouped 2 regions  

> select #4

2110 atoms, 2153 bonds, 1 model selected  

> show sel atoms

Grouped 2 regions  
Showing 2597 region surfaces  
Grouped 2 regions  

> select clear

Showing 2596 region surfaces  

> hide #!3 models

Showing 2596 region surfaces  

> save /home/priya/Priya_Projects/Msmegmatis_Ribosome_GH/50S+H68/50S+H68.cxs

opened ChimeraX session  

> select clear

> show #!3 models

> hide #!3 models

> show #!3 models

Showing 2596 region surfaces  

> select clear

> hide #!3 models

Grouped 6 regions  
Ungrouped to 6 regions  

> select clear

> select clear

Grouped 3 regions  
Grouped 2 regions  

> show #!3 models

> hide #!3 models

> show #!3 models

> hide #!3 models

Grouped 5 regions  

> select clear

Ungrouped to 4 regions  
Ungrouped to 0 regions  

> show #!3 models

> hide #!3 models

Ungrouped to 7 regions  
Ungrouped to 0 regions  

> select clear

> show #!3 models

> hide #!3 models

> show #!3 models

Grouped 6 regions  
Grouped 10 regions  
Grouped 3 regions  

> select clear

> hide #!3 models

Ungrouped to 3 regions  
Ungrouped to 0 regions  

> show #!3 models

> hide #!3 models

Ungrouped to 0 regions  

> show #!3 models

> hide #!3 models

Showing 2584 region surfaces  
Grouped 4 regions  

> select clear

> select clear

Grouped 15 regions  
Grouped 2 regions  
Grouped 2 regions  
Grouped 6 regions  
Grouped 2 regions  
Ungrouped to 2 regions  
Ungrouped to 9 regions  
Ungrouped to 12 regions  
Ungrouped to 2 regions  
Ungrouped to 18 regions  
Ungrouped to 16 regions  
Ungrouped to 35 regions  
Ungrouped to 12 regions  
Ungrouped to 0 regions  

> hide #4 models

Drag select of 3353, 3508, 3683, 3588, 1819, 3261, 3430, 2995, 3967, 4237,
3177, 3587, 2648, 2757, 2834, 1197, 978, 1085, 1612, 926, 1256, 1428, 923,
1024, 880, 791, 1892, 979, 1430, 2454, 1670, 1739, 1741, 1429, 1481, 2716,
2977, 2397, 2547, 1422, 1479, 1537, 1542, 1478, 1482, 1539, 2062, 2325, 1611,
1682, 1749, 1821, 1488, 1967, 2050, 2052, 2138, 2225, 1613, 1755, 1763, 1979,
2145, 2149, 2237, 2316, 2331, 1911, 713 of 1068 triangles, 1692, 877 of 940
triangles, 1902, 1982, 1985, 1832, 237 of 396 triangles, 1994, 298 of 424
triangles, 2083, 1830, 1091 of 1152 triangles, 1909, 2398, 2549, 2243  
Grouped 79 regions  
Showing 2560 region surfaces  

> select clear

> select clear

> show #!3 models

> hide #!3 models

Grouped 2 regions  

> show #4 models

> show #!3 models

> hide #!3 models

> show #!3 models

> hide #!3 models

Grouped 4 regions  

> show #!3 models

> hide #!3 models

> show #!3 models

> hide #!3 models

Grouped 5 regions  

> show #!3 models

> hide #!3 models

> show #!3 models

> hide #!3 models

> show #!3 models

> hide #!3 models

> show #!3 models

> hide #!3 models

> show #!3 models

> hide #!3 models

> show #!3 models

> hide #!3 models

Grouped 2 regions  

> select clear

> select clear

Ungrouped to 0 regions  

> show #!3 models

> hide #!3 models

Grouped 3 regions  
Showing 2549 region surfaces  
Grouped 2 regions  
Showing 2548 region surfaces  

> select clear

Ungrouped to 0 regions  

> hide #4 models

Grouped 3 regions  

> show #!3 models

> hide #!3 models

> show #!3 models

> hide #!3 models

> show #!3 models

> hide #!3 models

> show #!3 models

> hide #!3 models

Grouped 7 regions  
Ungrouped to 7 regions  
Grouped 6 regions  

> show #4 models

Grouped 3 regions  
Grouped 3 regions  
Ungrouped to 3 regions  
Ungrouped to 3 regions  
Ungrouped to 6 regions  
Ungrouped to 0 regions  

> show #!3 models

> hide #!3 models

> show #!3 models

Grouped 5 regions  
Grouped 3 regions  

> hide #!3 models

> select clear

> select clear

Ungrouped to 7 regions  
Ungrouped to 0 regions  
Ungrouped to 2 regions  
Ungrouped to 0 regions  

> select clear

Grouped 4 regions  

> show #!3 models

> hide #!3 models

Grouped 5 regions  

> select clear

Showing 2540 region surfaces  
Grouped 2 regions  
Showing 2539 region surfaces  

> select clear

> save /home/priya/Priya_Projects/Msmegmatis_Ribosome_GH/50S+H68/50S+H68.cxs

opened ChimeraX session  

> select clear

> show #!3 models

> hide #!3 models

> show #!3 models

> hide #!3 models

Grouped 5 regions  
Grouped 2 regions  
Grouped 2 regions  
Grouped 2 regions  
Showing 2532 region surfaces  
Grouped 2 regions  
Showing 2531 region surfaces  

> select clear

> select #4

2110 atoms, 2153 bonds, 1 model selected  

> hide sel atoms

> ~select #4

Nothing selected  
Grouped 3 regions  
Grouped 4 regions  

> select #4

2110 atoms, 2153 bonds, 1 model selected  
Grouped 6 regions  
Grouped 5 regions  
Showing 2517 region surfaces  
Grouped 2 regions  

> show sel atoms

> show sel atoms

> hide sel atoms

Showing 2516 region surfaces  
Grouped 5 regions  
Ungrouped to 5 regions  

> select clear

> select clear

Showing 2516 region surfaces  
Grouped 2 regions  

> select #4

2110 atoms, 2153 bonds, 1 model selected  

> show sel atoms

> hide sel atoms

> hide sel atoms

Showing 2515 region surfaces  

> select clear

Ungrouped to 5 regions  
Grouped 4 regions  

> select #4

2110 atoms, 2153 bonds, 1 model selected  

> show sel atoms

Ungrouped to 4 regions  
Grouped 4 regions  

> select clear

Showing 2516 region surfaces  
Grouped 2 regions  

> save /home/priya/Priya_Projects/Msmegmatis_Ribosome_GH/50S+H68/50S+H68.cxs

Showing 2515 region surfaces  

> select clear

> select clear

> show #!3 models

> hide #!3 models

Grouped 2 regions  
Drag select of 10194, 39984 of 43320 triangles, 1208 atoms, 268 residues, 1026
bonds  

> color (#4 & sel) red

> hide #4 models

> show #4 models

> select #4

2110 atoms, 2153 bonds, 1 model selected  

> ~select #4

Nothing selected  

> ui tool show "Color Actions"

> color sel lime

Showing 2514 region surfaces  

> select clear

> select clear

Showing 2514 region surfaces  
Grouped 3 regions  
Ungrouped to 3 regions  
Ungrouped to 2 regions  
Ungrouped to 7 regions  
Ungrouped to 2 regions  
Ungrouped to 6 regions  
Ungrouped to 10 regions  
Ungrouped to 10 regions  
Ungrouped to 9 regions  
Ungrouped to 7 regions  
Ungrouped to 10 regions  
Ungrouped to 21 regions  
Ungrouped to 2 regions  
Ungrouped to 4 regions  
Drag select of 3073, 2982, 2474, 2706, 2712, 2787, 2791, 2964, 2719, 2324,
2467, 2621, 2544, 1978, 2827, 2820, 2825, 2980, 2727, 2968, 2730, 2821, 2725,
2814, 2804, 2531, 2971, 2978, 3060, 3065, 3067, 2632, 2816, 2465, 2797, 2625,
2828, 2639, 2903, 2638, 2630, 2157, 2330, 2390, 2710, 2392, 2396, 2070, 2540,
2403, 2552, 2556, 2558, 2627, 2634, 2635, 2640, 2642, 2734, 2822, 2900, 2259,
3070, 2549, 2461, 2402, 10152, 31273 of 31276 triangles, 2551, 1265 atoms, 275
residues, 1073 bonds  

> select clear

Grouped 4 regions  
Grouped 4 regions  
Grouped 4 regions  

> select clear

Drag select of 3073, 2982, 2474, 2706, 2712, 2787, 2791, 2964, 2719, 2324,
2467, 2621, 2544, 1978, 2827, 2820, 2825, 2980, 2727, 2968, 2730, 2821, 2725,
2814, 2804, 2531, 2971, 2978, 3060, 3065, 3067, 2632, 2816, 2465, 2797, 2625,
2828, 2639, 2903, 2638, 2630, 2157, 2330, 2390, 2710, 2392, 2396, 2070, 2540,
2403, 2552, 2627, 2734, 2549, 391 of 432 triangles, 2461, 2402, 10152, 30916
of 31276 triangles, 2551, 10198, 1258 atoms, 274 residues, 1065 bonds  
Drag select of 3073, 2982, 2474, 2706, 332 of 356 triangles, 2712, 2787, 317
of 396 triangles, 2791, 746 of 752 triangles, 2964, 2719, 2324, 2467, 2621,
2544, 1978, 2827, 2820, 2825, 2980, 2727, 2968, 2730, 2821, 2725, 2814, 2804,
2531, 2971, 2978, 3060, 3065, 3067, 2632, 2816, 2465, 2797, 2625, 2828, 2639,
2903, 2638, 2630, 2157, 2330, 2390, 2710, 2392, 2396, 2070, 2540, 2403, 2552,
2627, 2734, 2549, 2461, 2402, 10152, 31154 of 31276 triangles, 2551, 1261
atoms, 275 residues, 1069 bonds  
Drag select of 3073, 2982, 2474, 2706, 2712, 2787, 2791, 2964, 2719, 2324,
2467, 2621, 2544, 1978, 2827, 2820, 2825, 2980, 2727, 2968, 2730, 2821, 2725,
2814, 2804, 2531, 2971, 2978, 3060, 3065, 3067, 2632, 2816, 2465, 2797, 2625,
2828, 2639, 2903, 2638, 2630, 2157, 2330, 2390, 2710, 2392, 2396, 2070, 2540,
2403, 2552, 2627, 2734, 2549, 2461, 2402, 10152, 2551, 1262 atoms, 275
residues, 1071 bonds  
Ungrouped to 5 regions  
Ungrouped to 5 regions  
Ungrouped to 3 regions  
Ungrouped to 2 regions  
Ungrouped to 4 regions  
Ungrouped to 4 regions  
Ungrouped to 6 regions  
Ungrouped to 2 regions  

> select clear

Drag select of 3073, 2982, 2474, 2706, 2712, 2787, 2791, 2964, 2719, 2324,
2467, 2621, 2544, 1978, 2827, 2820, 2825, 2980, 2727, 2968, 2730, 2821, 2725,
2814, 2804, 2531, 2971, 2978, 3060, 3065, 3067, 2632, 2816, 2465, 2797, 2625,
2828, 2639, 2903, 2638, 2630, 2157, 2330, 2390, 2710, 2392, 2396, 2070, 2540,
2403, 2552, 2627, 2734, 2549, 2461, 2402, 2551, 2476, 1769, 2071, 2236, 2557,
2735, 2985, 1825, 1691, 2746, 2837, 3077, 3082, 2561, 2412, 2647, 2563, 3083,
10139, 25931 of 26104 triangles, 8443, 1183 of 1200 triangles, 1261 atoms, 275
residues, 1069 bonds  

> select clear

Grouped 4 regions  

> select clear

Drag select of 3073, 2982, 2474, 2706, 2712, 2787, 2791, 2964, 2719, 2324,
2467, 2621, 2544, 1978, 2827, 2820, 2825, 2980, 2727, 2968, 2730, 2821, 2725,
2814, 2804, 2531, 2971, 2978, 3060, 3065, 3067, 2632, 2816, 2465, 2797, 2625,
2828, 2639, 2903, 2638, 2630, 2157, 2330, 2390, 2710, 2392, 2396, 2070, 2540,
2403, 2552, 2627, 2734, 2461, 2402, 2551, 2476, 1769, 414 of 572 triangles,
2071, 2236, 2557, 1825, 1691, 548 of 660 triangles, 2746, 2837, 3082, 2561,
2412, 2647, 2563, 3083, 10139, 26076 of 26104 triangles, 8443, 10199, 1258
atoms, 275 residues, 1067 bonds  

> select clear

Grouped 6 regions  
Drag select of 3073, 2982, 2474, 2719, 2324, 2467, 2621, 2544, 2827, 2820,
2825, 2980, 2727, 2968, 2730, 2821, 2725, 2814, 2804, 2531, 2971, 2978, 3060,
3065, 3067, 2632, 2816, 2465, 2797, 2625, 2828, 2639, 2903, 2638, 2630, 2157,
2330, 2390, 2710, 2392, 2396, 2070, 2540, 2403, 2552, 2627, 2734, 2461, 2402,
2551, 2476, 1769, 2071, 2236, 2557, 1825, 1691, 2746, 2837, 3082, 2561, 2412,
2647, 2563, 3083, 10139, 8443, 10200, 1265 atoms, 275 residues, 1073 bonds  

> select clear

Drag select of 3073, 2982, 2474, 2719, 2324, 2467, 2621, 2544, 2827, 2820,
2825, 2980, 2727, 2968, 2730, 2821, 2725, 2814, 2804, 2531, 2971, 2978, 3060,
3065, 3067, 2632, 2816, 2465, 2797, 2625, 2828, 2639, 2903, 2638, 2630, 2157,
2330, 2390, 2710, 2392, 2396, 2070, 2540, 2403, 2552, 2627, 2734, 2461, 2402,
2551, 2476, 1769, 2071, 2236, 2557, 1825, 1691, 2746, 2837, 337 of 368
triangles, 3082, 2561, 2412, 2647, 2563, 3083, 10139, 8443, 1166 of 1200
triangles, 10200, 1265 atoms, 275 residues, 1073 bonds  
Grouped 67 regions  

> save /home/priya/Priya_Projects/Msmegmatis_Ribosome_GH/50S+H68/50S+H68.cxs

Showing cryosparc_P1_J6_map_sharp.seg - 2515 regions, 2515 surfaces  
opened ChimeraX session  
Ungrouped to 67 regions  
Ungrouped to 4 regions  
Ungrouped to 2 regions  
Ungrouped to 2 regions  
Ungrouped to 2 regions  
Ungrouped to 2 regions  
Ungrouped to 2 regions  
Ungrouped to 2 regions  
Ungrouped to 3 regions  
Ungrouped to 2 regions  
Ungrouped to 2 regions  
Ungrouped to 2 regions  
Ungrouped to 3 regions  

> select clear

Grouped 3 regions  

> select clear

Drag select of 2330, 2621, 2465, 2820, 2630, 2978, 2727, 2980, 2638, 2825,
2828, 2746, 2396, 2531, 2324, 3073, 2403, 2827, 2625, 2412, 2968, 2390, 2461,
1691, 303 of 660 triangles, 2814, 2474, 2971, 3067, 2157, 2903, 2551, 2797,
2402, 2982, 2821, 2719, 2734, 2627, 2804, 2561, 2710, 1769, 169 of 572
triangles, 2552, 2557, 2725, 2236, 2392, 2476, 2837, 2540, 2632, 2544, 3082,
2467, 2639, 2647, 2070, 2816, 3083, 2071, 605 of 716 triangles, 2730, 1825,
908 of 960 triangles, 2563, 3171, 2911, 2478, 2753, 2410, 2486, 2624, 2815,
2733, 2546, 1439, 1372, 1262, 96 of 136 triangles, 1625, 10117, 21223 of 22960
triangles, 1775, 10202, 1205 atoms, 271 residues, 1019 bonds  
Grouped 79 regions  
Ungrouped to 79 regions  
Grouped 78 regions  
Showing 2517 region surfaces  

> select #4

2110 atoms, 2153 bonds, 1 model selected  

> ~select #4

Nothing selected  

> hide #4 models

> close #4

> save /home/priya/Priya_Projects/Msmegmatis_Ribosome_GH/50S+H68/50S+H68.cxs

opened ChimeraX session  

> open
> /home/priya/Priya_Projects/Msmegmatis_Ribosome_GH/50S+H68/Msm_23SrRNA.pdb

Chain information for Msm_23SrRNA.pdb #4  
---  
Chain | Description  
A | No description available  
  

> select #4

66981 atoms, 75001 bonds, 1 model selected  

> hide sel atoms

> open
> /home/priya/Priya_Projects/Msmegmatis_Ribosome_GH/50S+H68/Msm_L_Protein.pdb

Chain information for Msm_L_Protein.pdb #5  
---  
Chain | Description  
3 | No description available  
C | No description available  
D | No description available  
E | No description available  
F | No description available  
G | No description available  
H | No description available  
I | No description available  
J | No description available  
K | No description available  
L | No description available  
M | No description available  
N | No description available  
O | No description available  
P | No description available  
Q | No description available  
R | No description available  
S | No description available  
T | No description available  
U | No description available  
V | No description available  
W | No description available  
X | No description available  
Y | No description available  
Z | No description available  
a | No description available  
b | No description available  
c | No description available  
d | No description available  
e | No description available  
f | No description available  
g | No description available  
  

> style sel ball

Changed 66981 atom styles  

> select #5

28177 atoms, 28567 bonds, 2 pseudobonds, 2 models selected  

> style sel ball

Changed 28177 atom styles  

> style sel stick

Changed 28177 atom styles  

> hide sel atoms

> show sel cartoons

> ~select #5

Nothing selected  

> select clear

Ungrouped to 4 regions  
Ungrouped to 4 regions  
Ungrouped to 8 regions  
Ungrouped to 0 regions  
Grouped 9 regions  

> hide #4 models

> show #4 models

> hide #4 models

Grouped 10 regions  
Ungrouped to 10 regions  
Ungrouped to 27 regions  
Grouped 27 regions  
Grouped 22 regions  

> select clear

Showing 2497 region surfaces  
Grouped 2 regions  

> select clear

> save /home/priya/Priya_Projects/Msmegmatis_Ribosome_GH/50S+H68/50S+H68.cxs

Grouped 19 regions  
Grouped 24 regions  
Grouped 15 regions  
Grouped 7 regions  

> select clear

Grouped 24 regions  
Grouped 14 regions  
Grouped 3 regions  
Grouped 7 regions  
Grouped 12 regions  
Grouped 12 regions  
Grouped 5 regions  
Grouped 6 regions  
Showing 2360 region surfaces  
Grouped 2 regions  
Grouped 3 regions  
Grouped 2 regions  

> save /home/priya/Priya_Projects/Msmegmatis_Ribosome_GH/50S+H68/50S+H68.cxs

> select clear

Grouped 4 regions  

> select clear

Grouped 10 regions  
Grouped 18 regions  
Grouped 12 regions  
Grouped 10 regions  

> select clear

Grouped 3 regions  
Grouped 6 regions  
Grouped 5 regions  
Grouped 7 regions  
Grouped 3 regions  
Grouped 3 regions  
Showing 2286 region surfaces  

> select clear

> select clear

Grouped 3 regions  

> select clear

> save /home/priya/Priya_Projects/Msmegmatis_Ribosome_GH/50S+H68/50S+H68.cxs

Showing cryosparc_P1_J6_map_sharp.seg - 2284 regions, 2284 surfaces  
opened ChimeraX session  

> select clear

> select clear

Grouped 27 regions  
Grouped 5 regions  
Grouped 3 regions  
Grouped 5 regions  
Grouped 2 regions  

> save /home/priya/Priya_Projects/Msmegmatis_Ribosome_GH/50S+H68/50S+H68.cxs

> select clear

Ungrouped to 2 regions  
Ungrouped to 0 regions  
Ungrouped to 5 regions  
Ungrouped to 0 regions  
Grouped 4 regions  

> select clear

Ungrouped to 0 regions  
Ungrouped to 2 regions  
Ungrouped to 0 regions  
Ungrouped to 5 regions  
Ungrouped to 0 regions  

> select clear

Grouped 5 regions  
Showing 2250 region surfaces  
Grouped 3 regions  

> select clear

Grouped 4 regions  
Grouped 2 regions  

> select clear

Ungrouped to 8 regions  
Ungrouped to 0 regions  
Ungrouped to 2 regions  
Ungrouped to 0 regions  
Grouped 2 regions  
Grouped 4 regions  
Grouped 2 regions  

> hide #!1 models

> show #!1 models

> hide #!1 models

> show #!1 models

> hide #!1 models

> show #!1 models

Ungrouped to 2 regions  
Ungrouped to 4 regions  
Ungrouped to 10 regions  
Ungrouped to 2 regions  
Ungrouped to 4 regions  
Grouped 4 regions  
Grouped 2 regions  
Grouped 3 regions  
Grouped 3 regions  
Ungrouped to 0 regions  
Grouped 4 regions  
Grouped 2 regions  
Grouped 2 regions  
Showing 2249 region surfaces  

> save /home/priya/Priya_Projects/Msmegmatis_Ribosome_GH/50S+H68/50S+H68.cxs

Grouped 13 regions  
Grouped 1 regions  
Ungrouped to 1 regions  
Ungrouped to 13 regions  

> select clear

Grouped 8 regions  

> select clear

Ungrouped to 4 regions  
Ungrouped to 0 regions  
Ungrouped to 2 regions  
Grouped 10 regions  
Grouped 3 regions  

> hide #!1 models

> show #!1 models

Grouped 4 regions  
Grouped 2 regions  
Grouped 2 regions  
Grouped 6 regions  
Grouped 4 regions  

> save /home/priya/Priya_Projects/Msmegmatis_Ribosome_GH/50S+H68/50S+H68.cxs

> select clear

> hide #!5 models

> show #4 models

> show #!5 models

Grouped 9 regions  
Grouped 9 regions  

> select clear

Ungrouped to 0 regions  
Showing 2206 region surfaces  
Grouped 3 regions  

> hide #4 models

Showing 2204 region surfaces  
Grouped 4 regions  
Showing 2201 region surfaces  
Grouped 4 regions  
Ungrouped to 4 regions  

> select clear

> select clear

Grouped 3 regions  
Showing 2199 region surfaces  

> select clear

Grouped 2 regions  
Ungrouped to 2 regions  
Ungrouped to 7 regions  
Ungrouped to 7 regions  
Ungrouped to 9 regions  
Ungrouped to 4 regions  
Ungrouped to 12 regions  
Ungrouped to 10 regions  
Ungrouped to 2 regions  
Ungrouped to 4 regions  
Ungrouped to 9 regions  
Ungrouped to 14 regions  
Ungrouped to 5 regions  
Ungrouped to 0 regions  

> hide #!5 models

Drag select of 5395, 5405, 5503, 5582, 5774, 4991, 5189, 5191, 5965, 5870,
5973, 5577, 5766, 6068, 6170, 6173, 5759, 5501, 6070, 5872, 5575, 5678, 5583,
5681, 5770, 5873, 5399, 5401, 5498, 5769, 6178, 6180, 5496, 5676, 6633, 5089,
4866, 5483, 5491, 5865, 5675, 5764, 5970, 6455, 6556, 6370, 6636, 6697, 6704,
6460, 6558, 6269, 6362, 6176, 5972, 6554, 6555, 6629, 6361, 6366  

> show #!5 models

Grouped 57 regions  
Ungrouped to 57 regions  
Grouped 55 regions  
Ungrouped to 55 regions  
Grouped 54 regions  
Ungrouped to 54 regions  
Grouped 53 regions  
Grouped 2 regions  
Ungrouped to 2 regions  
Ungrouped to 53 regions  
Grouped 52 regions  
Grouped 3 regions  
Ungrouped to 3 regions  
Ungrouped to 52 regions  
Grouped 51 regions  
Grouped 2 regions  
Grouped 3 regions  
Ungrouped to 3 regions  
Ungrouped to 53 regions  
Ungrouped to 0 regions  

> hide #!5 models

Drag select of 5089, 6370, 6704, 6697, 6556, 5973, 5577, 5770, 5972, 4991,
6362, 6173, 5678, 6554, 6070, 5582, 6068, 5865, 5873, 5501, 6361, 6555, 5583,
5191, 5189, 6633, 6176, 5575, 6460, 5399, 5491, 6629, 5395, 6170, 5970, 5675,
5759, 6366, 6455, 6636, 6180, 5872, 6558, 5483, 4866, 5764, 5496, 6178, 5870,
5498, 5681, 5401, 5766, 6269  
Grouped 54 regions  

> show #4 models

> show #!5 models

> hide #4 models

Showing 2204 region surfaces  

> select clear

> select clear

> save /home/priya/Priya_Projects/Msmegmatis_Ribosome_GH/50S+H68/50S+H68.cxs

Grouped 13 regions  
Grouped 11 regions  
Grouped 2 regions  
Grouped 14 regions  

> select clear

> select clear

Grouped 5 regions  
Grouped 2 regions  
Grouped 4 regions  
Grouped 5 regions  
Grouped 5 regions  
Grouped 2 regions  

> select clear

Ungrouped to 4 regions  
Ungrouped to 0 regions  
Ungrouped to 7 regions  
Ungrouped to 0 regions  
Ungrouped to 6 regions  
Ungrouped to 0 regions  
Showing 2165 region surfaces  

> select clear

Grouped 3 regions  
Grouped 3 regions  

> select clear

Grouped 4 regions  
Showing 2158 region surfaces  
Grouped 2 regions  
Ungrouped to 2 regions  
Ungrouped to 7 regions  
Ungrouped to 5 regions  
Ungrouped to 2 regions  
Ungrouped to 5 regions  
Ungrouped to 16 regions  
Ungrouped to 7 regions  
Ungrouped to 9 regions  
Ungrouped to 11 regions  
Ungrouped to 6 regions  
Ungrouped to 0 regions  

> hide #!5 models

Drag select of 6346, 6435, 7036, 6156, 7394, 6755, 6437, 7323, 6973, 6688,
6825, 6971, 7146, 6751, 6162, 6256, 6258, 6534, 7037, 7097, 7194, 7232, 7321,
7098, 7276, 7147, 7192, 6753, 6750, 6901, 6827, 6341, 6344, 6434, 6620, 6622,
6754, 6160, 6432, 6822, 6153, 6821, 6900, 6970, 6527, 6685, 6686, 6155, 6157,
5663, 5746, 5845, 5953  

> show #!5 models

Grouped 48 regions  
Ungrouped to 48 regions  
Grouped 48 regions  
Ungrouped to 48 regions  
Grouped 47 regions  
Grouped 2 regions  
Ungrouped to 2 regions  
Ungrouped to 47 regions  
Grouped 47 regions  
Grouped 2 regions  
Showing 2162 region surfaces  

> color #1.540 cyan

> select clear

> hide #!1 models

> show #!1 models

Grouped 19 regions  
Grouped 2 regions  
Showing 2143 region surfaces  
Grouped 2 regions  
Grouped 2 regions  

> select clear

Grouped 29 regions  
Grouped 17 regions  
Grouped 10 regions  
Grouped 7 regions  
Grouped 7 regions  
Grouped 10 regions  
Grouped 10 regions  
Grouped 12 regions  
Grouped 16 regions  
Grouped 8 regions  
Grouped 8 regions  
Grouped 4 regions  
Grouped 8 regions  
Grouped 6 regions  
Grouped 8 regions  
Grouped 5 regions  
Grouped 2 regions  
Grouped 2 regions  
Grouped 18 regions  
Showing 1973 region surfaces  
Grouped 2 regions  

> save /home/priya/Priya_Projects/Msmegmatis_Ribosome_GH/50S+H68/50S+H68.cxs

> select clear

Traceback (most recent call last):  
File "/opt/UCSF/ChimeraX/lib/python3.7/site-
packages/chimerax/segger/segment_dialog.py", line 958, in ColorDensity  
smod.color_density()  
File "/opt/UCSF/ChimeraX/lib/python3.7/site-
packages/chimerax/segger/regions.py", line 851, in color_density  
color_surface_pieces(d.surfaces, m, ijk_to_xyz, offset, cmap)  
File "/opt/UCSF/ChimeraX/lib/python3.7/site-
packages/chimerax/segger/regions.py", line 1886, in color_surface_pieces  
xyz = v - offset*n  
TypeError: unsupported operand type(s) for *: 'float' and 'NoneType'  
  
TypeError: unsupported operand type(s) for *: 'float' and 'NoneType'  
  
File "/opt/UCSF/ChimeraX/lib/python3.7/site-
packages/chimerax/segger/regions.py", line 1886, in color_surface_pieces  
xyz = v - offset*n  
  
See log for complete Python traceback.  
  




OpenGL version: 3.3.0 NVIDIA 390.132
OpenGL renderer: Quadro 2000/PCIe/SSE2
OpenGL vendor: NVIDIA Corporation
Manufacturer: Dell Inc.
Model: Precision WorkStation T7500
OS: CentOS Linux 7 Core
Architecture: 64bit ELF
CPU: 12 Intel(R) Xeon(R) CPU           X5690  @ 3.47GHz
Cache Size: 12288 KB
Memory:
	              total        used        free      shared  buff/cache   available
	Mem:            94G        5.7G        1.1G        115M         87G         87G
	Swap:          4.0G        733M        3.3G

Graphics:
	03:00.0 VGA compatible controller [0300]: NVIDIA Corporation GF106GL [Quadro 2000] [10de:0dd8] (rev a1)	
	Subsystem: NVIDIA Corporation Device [10de:084a]	
	Kernel driver in use: nvidia
PyQt version: 5.12.3
Compiled Qt version: 5.12.4
Runtime Qt version: 5.12.8

Change History (2)

comment:1 by Eric Pettersen, 4 years ago

Component: UnassignedVolume Data
Owner: set to Tom Goddard
Platform: all
Project: ChimeraX
Status: newassigned
Summary: ChimeraX bug report submissionSegger: n is None

comment:2 by Tom Goddard, 4 years ago

Resolution: can't reproduce
Status: assignedclosed

User tried Segger menu Regions / Color Density Map and it was trying to color a volume surface but the normal vectors array was None. I don't see any way a volume surface can have normals array that is None. It could be the volume was closed but the current segger code checks for that. Not sure if the ChimeraX 1.0 Segger code checked that. I tried to reproduce this error with an empty volume surface with threshold level above highest map value. Did not cause an error, normals array is zero length but not None.

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