Opened 4 weeks ago

Closed 4 weeks ago

#19310 closed defect (duplicate)

Orthoplanes: 'NoneType' object has no attribute 'image_colors'

Reported by: chimerax-bug-report@… Owned by: Zach Pearson
Priority: normal Milestone:
Component: DICOM Version:
Keywords: Cc:
Blocked By: Blocking:
Notify when closed: Platform: all
Project: ChimeraX

Description (last modified by Eric Pettersen)

The following bug report has been submitted:
Platform:        macOS-26.0.1-arm64-arm-64bit
ChimeraX Version: 1.11.dev202509040038 (2025-09-04 00:38:56 UTC)
Description
Replace this text with list of actions that caused this problem to occur

Log:
UCSF ChimeraX version: 1.11.dev202509040038 (2025-09-04)  
© 2016-2025 Regents of the University of California. All rights reserved.  

> open
> /Users/madhurikanavalli/Desktop/2025/Cryosparc/Q387R/Tetramer_goodmodel_01_05Nov25.cxs
> format session

Opened cryosparc_P1_J482_004_volume_map.mrc as #2, grid size 352,352,352,
pixel 0.846, shown at level 0.0906, step 2, values float32  
Log from Wed Nov 5 23:47:55 2025UCSF ChimeraX version: 1.11.dev202509040038
(2025-09-04)  
© 2016-2025 Regents of the University of California. All rights reserved.  

> open
> /Users/madhurikanavalli/Desktop/2025/Cryosparc/Q387R/tetramer_02_withouttickoncopymaps.cxs
> format session

Opened cryosparc_P1_J482_004_volume_map.mrc as #2, grid size 352,352,352,
pixel 0.846, shown at level 0.0906, step 2, values float32  
Log from Wed Nov 5 22:22:09 2025UCSF ChimeraX version: 1.11.dev202509040038
(2025-09-04)  
© 2016-2025 Regents of the University of California. All rights reserved.  

> open /Users/madhurikanavalli/Desktop/test/dimerfit_Kasia.cxs

Error opening map "/Users/madhurikanavalli/3u1k.cif": File 3u1k.cif, format
mrc  
MRC header value nsymbt (1869770030) is invalid  
  

restore_snapshot for "Volume" returned None  

restore_snapshot for "VolumeSurface" returned None  

Log from Fri Aug 30 15:18:41 2024 Startup Messages  
---  
warnings | Replacing fetcher for 'ngff' and format OME-Zarr from OME-Zarr bundle with that from OME-Zarr bundle  
Replacing fetcher for 'pdb_nmr' and format NMRSTAR from NMRSTAR bundle with
that from NMRSTAR bundle  
  
UCSF ChimeraX version: 1.7 (2023-12-19)  
© 2016-2023 Regents of the University of California. All rights reserved.  
How to cite UCSF ChimeraX  

> open "/Users/madhurikanavalli/Downloads/cryosparc_P1_J138_004_volume_map
> (1).mrc" format mrc

No such file/path:
/Users/madhurikanavalli/Downloads/cryosparc_P1_J138_004_volume_map (1).mrc  

> open "/Users/madhurikanavalli/Downloads/AF-Q8TCS8-F1-model_v4 (1).pdb"

AF-Q8TCS8-F1-model_v4 (1).pdb title:  
Alphafold monomer V2.0 prediction for polyribonucleotide
nucleotidyltransferase 1, mitochondrial (Q8TCS8) [more info...]  
  
Chain information for AF-Q8TCS8-F1-model_v4 (1).pdb #1  
---  
Chain | Description | UniProt  
A | polyribonucleotide nucleotidyltransferase 1, mitochondrial | PNPT1_HUMAN 1-783  
  

> open
> /Users/madhurikanavalli/Downloads/cryosparc_P1_J138_004_volume_map_sharp.mrc

Opened cryosparc_P1_J138_004_volume_map_sharp.mrc as #2, grid size
352,352,352, pixel 0.846, shown at level 0.062, step 2, values float32  

> hide #1 models

> close #1

> volume #2 level 0.0941

> open /Users/madhurikanavalli/Desktop/ImageProcessing/5zf6.pdb

5zf6.pdb title:  
Crystal structure of the dimeric human pnpase [more info...]  
  
Chain information for 5zf6.pdb #1  
---  
Chain | Description | UniProt  
A B | polyribonucleotide nucleotidyltransferase 1, mitochondrial | PNPT1_HUMAN 46-669  
  

> transparency 50

> lighting soft

> lighting full

> lighting soft

> select add #2

2 models selected  

> hide #!2 models

> show #!2 models

> select subtract #2

Nothing selected  

> select add #1

8555 atoms, 8618 bonds, 1174 residues, 1 model selected  

> ui mousemode right "rotate selected models"

> view matrix models
> #1,0.56827,-0.43732,-0.69701,64.748,0.67429,0.73298,0.089865,-55.904,0.47159,-0.52106,0.7114,-23.186

> ui mousemode right "translate selected models"

> view matrix models
> #1,0.56827,-0.43732,-0.69701,66.837,0.67429,0.73298,0.089865,-22.52,0.47159,-0.52106,0.7114,54.916

> ui mousemode right "rotate selected models"

> view matrix models
> #1,-0.81909,0.51893,0.24453,138.84,-0.017183,-0.44828,0.89373,21.948,0.5734,0.72785,0.3761,42.804

> view matrix models
> #1,-0.69127,0.68715,0.22353,126.9,0.39929,0.10542,0.91074,-19.908,0.60225,0.71883,-0.34725,65.002

> ui mousemode right "translate selected models"

[Repeated 1 time(s)]

> view matrix models
> #1,-0.69127,0.68715,0.22353,135.47,0.39929,0.10542,0.91074,90.055,0.60225,0.71883,-0.34725,57.169

> view matrix models
> #1,-0.69127,0.68715,0.22353,190.44,0.39929,0.10542,0.91074,101.05,0.60225,0.71883,-0.34725,59.234

> view matrix models
> #1,-0.69127,0.68715,0.22353,191.18,0.39929,0.10542,0.91074,83.012,0.60225,0.71883,-0.34725,60.929

> ui tool show "Fit in Map"

> fitmap #1 inMap #2

Fit molecule 5zf6.pdb (#1) to map cryosparc_P1_J138_004_volume_map_sharp.mrc
(#2) using 8555 atoms  
average map value = 0.1101, steps = 196  
shifted from previous position = 5.16  
rotated from previous position = 14.4 degrees  
atoms outside contour = 4870, contour level = 0.0941  
  
Position of 5zf6.pdb (#1) relative to
cryosparc_P1_J138_004_volume_map_sharp.mrc (#2) coordinates:  
Matrix rotation and translation  
-0.53017153 0.78333170 0.32451439 175.24914037  
0.58899671 0.06493505 0.80552239 67.47116186  
0.60991887 0.61820295 -0.49580651 64.01082743  
Axis -0.47685384 -0.72654616 -0.49471314  
Axis point 66.66011786 0.00000000 15.64936506  
Rotation angle (degrees) 168.67279119  
Shift along axis -164.25613670  
  

> open /Users/madhurikanavalli/Downloads/5zf6.pdb

5zf6.pdb title:  
Crystal structure of the dimeric human pnpase [more info...]  
  
Chain information for 5zf6.pdb #3  
---  
Chain | Description | UniProt  
A B | polyribonucleotide nucleotidyltransferase 1, mitochondrial | PNPT1_HUMAN 46-669  
  

> select add #3

17110 atoms, 17236 bonds, 2348 residues, 2 models selected  

> ui mousemode right "rotate selected models"

> select subtract #1

8555 atoms, 8618 bonds, 1174 residues, 1 model selected  

> ui mousemode right "rotate selected models"

> view matrix models
> #3,-0.685,0.71791,0.124,127.29,-0.51455,-0.59723,0.61526,41.137,0.51576,0.35765,0.77851,-39.683

> view matrix models
> #3,-0.40337,0.91499,-0.0086238,106,0.40236,0.18583,0.89642,-54.028,0.82182,0.35812,-0.44312,-23.78

> ui mousemode right "translate selected models"

> ui mousemode right "rotate selected models"

> view matrix models
> #3,-0.76019,0.5729,-0.30642,149.86,-0.04323,-0.51519,-0.85599,50.769,-0.64826,-0.63747,0.41641,81.316

> ui mousemode right "translate selected models"

> view matrix models
> #3,-0.76019,0.5729,-0.30642,192.58,-0.04323,-0.51519,-0.85599,144.15,-0.64826,-0.63747,0.41641,196.86

> view matrix models
> #3,-0.76019,0.5729,-0.30642,192.59,-0.04323,-0.51519,-0.85599,144.19,-0.64826,-0.63747,0.41641,196.87

> view matrix models
> #3,-0.76019,0.5729,-0.30642,209.85,-0.04323,-0.51519,-0.85599,187.07,-0.64826,-0.63747,0.41641,235.74

> ui mousemode right "rotate selected models"

> view matrix models
> #3,-0.75983,0.62964,-0.16191,204.25,-0.13348,-0.39483,-0.90901,194.93,-0.63628,-0.66908,0.38404,236.22

> ui mousemode right "translate selected models"

> view matrix models
> #3,-0.75983,0.62964,-0.16191,211.78,-0.13348,-0.39483,-0.90901,195.69,-0.63628,-0.66908,0.38404,232.58

> select add #1

17110 atoms, 17236 bonds, 2348 residues, 2 models selected  

> select subtract #1

8555 atoms, 8618 bonds, 1174 residues, 1 model selected  

> select add #2

8555 atoms, 8618 bonds, 1174 residues, 3 models selected  

> fitmap #1 inMap #2

Fit molecule 5zf6.pdb (#1) to map cryosparc_P1_J138_004_volume_map_sharp.mrc
(#2) using 8555 atoms  
average map value = 0.1101, steps = 120  
shifted from previous position = 0.0129  
rotated from previous position = 0.00865 degrees  
atoms outside contour = 4870, contour level = 0.0941  
  
Position of 5zf6.pdb (#1) relative to
cryosparc_P1_J138_004_volume_map_sharp.mrc (#2) coordinates:  
Matrix rotation and translation  
-0.53020886 0.78332789 0.32446259 175.25676778  
0.58887934 0.06490056 0.80561097 67.48406251  
0.60999974 0.61821139 -0.49569646 63.98889300  
Axis -0.47682626 -0.72653104 -0.49476193  
Axis point 66.66315130 -0.00000000 15.63552486  
Rotation angle (degrees) 168.66721693  
Shift along axis -164.25556328  
  

> fitmap #1 inMap #2

Fit molecule 5zf6.pdb (#1) to map cryosparc_P1_J138_004_volume_map_sharp.mrc
(#2) using 8555 atoms  
average map value = 0.1101, steps = 1156  
shifted from previous position = 0.0206  
rotated from previous position = 0.00782 degrees  
atoms outside contour = 4870, contour level = 0.0941  
  
Position of 5zf6.pdb (#1) relative to
cryosparc_P1_J138_004_volume_map_sharp.mrc (#2) coordinates:  
Matrix rotation and translation  
-0.53016613 0.78336587 0.32444072 175.24849006  
0.58881022 0.06481730 0.80566819 67.49077469  
0.61010360 0.61817200 -0.49561777 63.99770693  
Axis -0.47684108 -0.72649898 -0.49479473  
Axis point 66.65580950 0.00000000 15.63768479  
Rotation angle (degrees) 168.66165391  
Shift along axis -164.26338558  
  

> fitmap #1 inMap #2

Fit molecule 5zf6.pdb (#1) to map cryosparc_P1_J138_004_volume_map_sharp.mrc
(#2) using 8555 atoms  
average map value = 0.1094, steps = 2000  
shifted from previous position = 0.221  
rotated from previous position = 0.156 degrees  
atoms outside contour = 4865, contour level = 0.0941  
  
Position of 5zf6.pdb (#1) relative to
cryosparc_P1_J138_004_volume_map_sharp.mrc (#2) coordinates:  
Matrix rotation and translation  
-0.52957137 0.78285031 0.32664898 175.11992042  
0.59076847 0.06403733 0.80429586 67.16427833  
0.60872554 0.61890598 -0.49639562 64.14667933  
Axis -0.47735255 -0.72630697 -0.49458339  
Axis point 66.63691808 0.00000000 15.73667756  
Rotation angle (degrees) 168.80285345  
Shift along axis -164.10170605  
  

> fitmap #1 inMap #2

Fit molecule 5zf6.pdb (#1) to map cryosparc_P1_J138_004_volume_map_sharp.mrc
(#2) using 8555 atoms  
average map value = 0.1101, steps = 288  
shifted from previous position = 0.225  
rotated from previous position = 0.154 degrees  
atoms outside contour = 4870, contour level = 0.0941  
  
Position of 5zf6.pdb (#1) relative to
cryosparc_P1_J138_004_volume_map_sharp.mrc (#2) coordinates:  
Matrix rotation and translation  
-0.53019581 0.78335894 0.32440896 175.25546840  
0.58887005 0.06493539 0.80561496 67.48903760  
0.61002006 0.61816840 -0.49572509 63.99117885  
Axis -0.47682923 -0.72654156 -0.49474361  
Axis point 66.66110864 0.00000000 15.63745351  
Rotation angle (degrees) 168.66440970  
Shift along axis -164.25974808  
  

> fitmap #1 inMap #2

Fit molecule 5zf6.pdb (#1) to map cryosparc_P1_J138_004_volume_map_sharp.mrc
(#2) using 8555 atoms  
average map value = 0.1101, steps = 232  
shifted from previous position = 0.00354  
rotated from previous position = 0.00252 degrees  
atoms outside contour = 4871, contour level = 0.0941  
  
Position of 5zf6.pdb (#1) relative to
cryosparc_P1_J138_004_volume_map_sharp.mrc (#2) coordinates:  
Matrix rotation and translation  
-0.53020339 0.78336172 0.32438985 175.25781259  
0.58883600 0.06492688 0.80564053 67.48885321  
0.61004634 0.61816576 -0.49569604 63.99054098  
Axis -0.47682257 -0.72653754 -0.49475594  
Axis point 66.66291520 -0.00000000 15.63787915  
Rotation angle (degrees) 168.66252058  
Shift along axis -164.25976573  
  

> fitmap #1 inMap #2

Fit molecule 5zf6.pdb (#1) to map cryosparc_P1_J138_004_volume_map_sharp.mrc
(#2) using 8555 atoms  
average map value = 0.1101, steps = 256  
shifted from previous position = 0.0121  
rotated from previous position = 0.00748 degrees  
atoms outside contour = 4870, contour level = 0.0941  
  
Position of 5zf6.pdb (#1) relative to
cryosparc_P1_J138_004_volume_map_sharp.mrc (#2) coordinates:  
Matrix rotation and translation  
-0.53021450 0.78340257 0.32427304 175.25068399  
0.58880860 0.06502852 0.80565236 67.48701037  
0.61006313 0.61810331 -0.49575323 63.99276039  
Axis -0.47680987 -0.72657013 -0.49472032  
Axis point 66.66124227 0.00000000 15.64470854  
Rotation angle (degrees) 168.65766312  
Shift along axis -164.25382012  
  

> fitmap #1 inMap #2

Fit molecule 5zf6.pdb (#1) to map cryosparc_P1_J138_004_volume_map_sharp.mrc
(#2) using 8555 atoms  
average map value = 0.1101, steps = 592  
shifted from previous position = 0.0158  
rotated from previous position = 0.0135 degrees  
atoms outside contour = 4866, contour level = 0.0941  
  
Position of 5zf6.pdb (#1) relative to
cryosparc_P1_J138_004_volume_map_sharp.mrc (#2) coordinates:  
Matrix rotation and translation  
-0.53013987 0.78336565 0.32448417 175.24674236  
0.58888115 0.06482254 0.80561593 67.48555859  
0.61005796 0.61817174 -0.49567428 64.00228754  
Axis -0.47686010 -0.72650285 -0.49477071  
Axis point 66.65490167 0.00000000 15.64048878  
Rotation angle (degrees) 168.66529813  
Shift along axis -164.26308627  
  

> fitmap #1 inMap #2

Fit molecule 5zf6.pdb (#1) to map cryosparc_P1_J138_004_volume_map_sharp.mrc
(#2) using 8555 atoms  
average map value = 0.1101, steps = 424  
shifted from previous position = 0.0048  
rotated from previous position = 0.00415 degrees  
atoms outside contour = 4866, contour level = 0.0941  
  
Position of 5zf6.pdb (#1) relative to
cryosparc_P1_J138_004_volume_map_sharp.mrc (#2) coordinates:  
Matrix rotation and translation  
-0.53018618 0.78335667 0.32443018 175.25208806  
0.58883675 0.06487179 0.80564442 67.48445550  
0.61006056 0.61817795 -0.49566332 63.99813793  
Axis -0.47683273 -0.72651883 -0.49477363  
Axis point 66.66006680 0.00000000 15.64100041  
Rotation angle (degrees) 168.66327461  
Shift along axis -164.25925025  
  

> fitmap #1 inMap #2

Fit molecule 5zf6.pdb (#1) to map cryosparc_P1_J138_004_volume_map_sharp.mrc
(#2) using 8555 atoms  
average map value = 0.11, steps = 2000  
shifted from previous position = 0.0762  
rotated from previous position = 0.165 degrees  
atoms outside contour = 4884, contour level = 0.0941  
  
Position of 5zf6.pdb (#1) relative to
cryosparc_P1_J138_004_volume_map_sharp.mrc (#2) coordinates:  
Matrix rotation and translation  
-0.53048495 0.78362459 0.32329279 175.29799081  
0.58666724 0.06409689 0.80728752 67.68716888  
0.61188829 0.61791917 -0.49372931 63.80010561  
Axis -0.47649839 -0.72617881 -0.49559421  
Axis point 66.65973389 0.00000000 15.50596390  
Rotation angle (degrees) 168.53856685  
Shift along axis -164.30116055  
  

> fitmap #1 inMap #2

Fit molecule 5zf6.pdb (#1) to map cryosparc_P1_J138_004_volume_map_sharp.mrc
(#2) using 8555 atoms  
average map value = 0.1101, steps = 2000  
shifted from previous position = 0.0839  
rotated from previous position = 0.144 degrees  
atoms outside contour = 4865, contour level = 0.0941  
  
Position of 5zf6.pdb (#1) relative to
cryosparc_P1_J138_004_volume_map_sharp.mrc (#2) coordinates:  
Matrix rotation and translation  
-0.53008745 0.78332676 0.32466363 175.23978220  
0.58861806 0.06432765 0.80584783 67.49400517  
0.61035733 0.61827270 -0.49517956 63.99461111  
Axis -0.47687716 -0.72632656 -0.49501302  
Axis point 66.64699995 0.00000000 15.62216883  
Rotation angle (degrees) 168.65768665  
Shift along axis -164.26870487  
  

> fitmap #1 inMap #2

Fit molecule 5zf6.pdb (#1) to map cryosparc_P1_J138_004_volume_map_sharp.mrc
(#2) using 8555 atoms  
average map value = 0.1101, steps = 644  
shifted from previous position = 0.0503  
rotated from previous position = 0.0463 degrees  
atoms outside contour = 4866, contour level = 0.0941  
  
Position of 5zf6.pdb (#1) relative to
cryosparc_P1_J138_004_volume_map_sharp.mrc (#2) coordinates:  
Matrix rotation and translation  
-0.53018602 0.78335265 0.32444013 175.24810216  
0.58896280 0.06498915 0.80554282 67.47147274  
0.60993902 0.61817071 -0.49582191 64.00371416  
Axis -0.47684122 -0.72656297 -0.49470061  
Axis point 66.66109101 0.00000000 15.64899309  
Rotation angle (degrees) 168.66926053  
Shift along axis -164.25046913  
  

> select subtract #2

8555 atoms, 8618 bonds, 1174 residues, 1 model selected  

> select subtract #3

Nothing selected  

> fitmap #1 inMap #2

Fit molecule 5zf6.pdb (#1) to map cryosparc_P1_J138_004_volume_map_sharp.mrc
(#2) using 8555 atoms  
average map value = 0.1101, steps = 536  
shifted from previous position = 0.00749  
rotated from previous position = 0.0178 degrees  
atoms outside contour = 4870, contour level = 0.0941  
  
Position of 5zf6.pdb (#1) relative to
cryosparc_P1_J138_004_volume_map_sharp.mrc (#2) coordinates:  
Matrix rotation and translation  
-0.53026340 0.78337387 0.32426241 175.26231884  
0.58871526 0.06498576 0.80572402 67.49295778  
0.61011070 0.61814418 -0.49564372 63.98242363  
Axis -0.47678032 -0.72655381 -0.49477277  
Axis point 66.66763886 0.00000000 15.63730709  
Rotation angle (degrees) 168.65506434  
Shift along axis -164.25565097  
  

> fitmap #1 inMap #2

Fit molecule 5zf6.pdb (#1) to map cryosparc_P1_J138_004_volume_map_sharp.mrc
(#2) using 8555 atoms  
average map value = 0.1101, steps = 1228  
shifted from previous position = 0.0152  
rotated from previous position = 0.0139 degrees  
atoms outside contour = 4868, contour level = 0.0941  
  
Position of 5zf6.pdb (#1) relative to
cryosparc_P1_J138_004_volume_map_sharp.mrc (#2) coordinates:  
Matrix rotation and translation  
-0.53017306 0.78334486 0.32448013 175.24906746  
0.58880624 0.06477853 0.80567422 67.49336568  
0.61010142 0.61820269 -0.49558217 63.99493089  
Axis -0.47683947 -0.72648633 -0.49481484  
Axis point 66.65525002 0.00000000 15.63335671  
Rotation angle (degrees) 168.66312652  
Shift along axis -164.26432228  
  

> select add #3

8555 atoms, 8618 bonds, 1174 residues, 1 model selected  

> view matrix models
> #3,-0.75983,0.62964,-0.16191,211.45,-0.13348,-0.39483,-0.90901,195.61,-0.63628,-0.66908,0.38404,229.67

> fitmap #1 inMap #2

Fit molecule 5zf6.pdb (#1) to map cryosparc_P1_J138_004_volume_map_sharp.mrc
(#2) using 8555 atoms  
average map value = 0.1101, steps = 1612  
shifted from previous position = 0.00572  
rotated from previous position = 0.00235 degrees  
atoms outside contour = 4866, contour level = 0.0941  
  
Position of 5zf6.pdb (#1) relative to
cryosparc_P1_J138_004_volume_map_sharp.mrc (#2) coordinates:  
Matrix rotation and translation  
-0.53017195 0.78332985 0.32451818 175.25199022  
0.58882446 0.06475693 0.80566264 67.48991761  
0.61008479 0.61822398 -0.49557609 63.99297271  
Axis -0.47684301 -0.72648001 -0.49482072  
Axis point 66.65742493 0.00000000 15.63211166  
Rotation angle (degrees) 168.66522554  
Shift along axis -164.26281069  
  

> fitmap #1 inMap #2

Fit molecule 5zf6.pdb (#1) to map cryosparc_P1_J138_004_volume_map_sharp.mrc
(#2) using 8555 atoms  
average map value = 0.1101, steps = 1316  
shifted from previous position = 0.0116  
rotated from previous position = 0.00894 degrees  
atoms outside contour = 4869, contour level = 0.0941  
  
Position of 5zf6.pdb (#1) relative to
cryosparc_P1_J138_004_volume_map_sharp.mrc (#2) coordinates:  
Matrix rotation and translation  
-0.53019294 0.78333241 0.32447772 175.24972531  
0.58889169 0.06488422 0.80560326 67.47923889  
0.61000166 0.61820739 -0.49569910 63.99592466  
Axis -0.47683532 -0.72652561 -0.49476117  
Axis point 66.66002577 0.00000000 15.63982371  
Rotation angle (degrees) 168.66766242  
Shift along axis -164.25335202  
  

> fitmap #1 inMap #2

Fit molecule 5zf6.pdb (#1) to map cryosparc_P1_J138_004_volume_map_sharp.mrc
(#2) using 8555 atoms  
average map value = 0.1099, steps = 2000  
shifted from previous position = 0.176  
rotated from previous position = 0.345 degrees  
atoms outside contour = 4900, contour level = 0.0941  
  
Position of 5zf6.pdb (#1) relative to
cryosparc_P1_J138_004_volume_map_sharp.mrc (#2) coordinates:  
Matrix rotation and translation  
-0.53086010 0.78435805 0.32088940 175.35137069  
0.58432492 0.06452559 0.80895045 67.89601905  
0.61380122 0.61694319 -0.49257402 63.64064322  
Axis -0.47605165 -0.72622856 -0.49595051  
Axis point 66.67562044 0.00000000 15.44865974  
Rotation angle (degrees) 168.36557671  
Shift along axis -164.34694642  
  

> fitmap #1 inMap #2

Fit molecule 5zf6.pdb (#1) to map cryosparc_P1_J138_004_volume_map_sharp.mrc
(#2) using 8555 atoms  
average map value = 0.1101, steps = 448  
shifted from previous position = 0.18  
rotated from previous position = 0.346 degrees  
atoms outside contour = 4871, contour level = 0.0941  
  
Position of 5zf6.pdb (#1) relative to
cryosparc_P1_J138_004_volume_map_sharp.mrc (#2) coordinates:  
Matrix rotation and translation  
-0.53022396 0.78333692 0.32441613 175.25810721  
0.58890184 0.06498377 0.80558781 67.48045204  
0.60996489 0.61819122 -0.49576451 63.99022697  
Axis -0.47681825 -0.72655995 -0.49472719  
Axis point 66.66563091 0.00000000 15.63997000  
Rotation angle (degrees) 168.66720688  
Shift along axis -164.25256331  
  

> fitmap #1 inMap #2

Fit molecule 5zf6.pdb (#1) to map cryosparc_P1_J138_004_volume_map_sharp.mrc
(#2) using 8555 atoms  
average map value = 0.1101, steps = 608  
shifted from previous position = 0.00455  
rotated from previous position = 0.00172 degrees  
atoms outside contour = 4871, contour level = 0.0941  
  
Position of 5zf6.pdb (#1) relative to
cryosparc_P1_J138_004_volume_map_sharp.mrc (#2) coordinates:  
Matrix rotation and translation  
-0.53022740 0.78334400 0.32439340 175.25531311  
0.58888033 0.06498821 0.80560318 67.48409621  
0.60998267 0.61818178 -0.49575440 63.98898197  
Axis -0.47681416 -0.72656058 -0.49473021  
Axis point 66.66354227 0.00000000 15.63878071  
Rotation angle (degrees) 168.66558797  
Shift along axis -164.25278098  
  

> fitmap #1 inMap #2

Fit molecule 5zf6.pdb (#1) to map cryosparc_P1_J138_004_volume_map_sharp.mrc
(#2) using 8555 atoms  
average map value = 0.1101, steps = 952  
shifted from previous position = 0.0198  
rotated from previous position = 0.0253 degrees  
atoms outside contour = 4864, contour level = 0.0941  
  
Position of 5zf6.pdb (#1) relative to
cryosparc_P1_J138_004_volume_map_sharp.mrc (#2) coordinates:  
Matrix rotation and translation  
-0.53016383 0.78326739 0.32468217 175.24708520  
0.58882646 0.06458350 0.80567510 67.48844552  
0.61008992 0.61832125 -0.49544840 63.98628800  
Axis -0.47685500 -0.72642282 -0.49489311  
Axis point 66.65381088 0.00000000 15.62105041  
Rotation angle (degrees) 168.67071089  
Shift along axis -164.25896936  
  

> fitmap #1 inMap #2

Fit molecule 5zf6.pdb (#1) to map cryosparc_P1_J138_004_volume_map_sharp.mrc
(#2) using 8555 atoms  
average map value = 0.1101, steps = 64  
shifted from previous position = 0.0148  
rotated from previous position = 0.0251 degrees  
atoms outside contour = 4870, contour level = 0.0941  
  
Position of 5zf6.pdb (#1) relative to
cryosparc_P1_J138_004_volume_map_sharp.mrc (#2) coordinates:  
Matrix rotation and translation  
-0.53020351 0.78337851 0.32434911 175.25525365  
0.58883378 0.06496663 0.80563894 67.49212990  
0.61004836 0.61814032 -0.49572527 63.98995244  
Axis -0.47682037 -0.72655051 -0.49473902  
Axis point 66.66105482 0.00000000 15.63827816  
Rotation angle (degrees) 168.66100586  
Shift along axis -164.26004271  
  

> fitmap #1 inMap #2

Fit molecule 5zf6.pdb (#1) to map cryosparc_P1_J138_004_volume_map_sharp.mrc
(#2) using 8555 atoms  
average map value = 0.11, steps = 2000  
shifted from previous position = 0.0976  
rotated from previous position = 0.221 degrees  
atoms outside contour = 4868, contour level = 0.0941  
  
Position of 5zf6.pdb (#1) relative to
cryosparc_P1_J138_004_volume_map_sharp.mrc (#2) coordinates:  
Matrix rotation and translation  
-0.53030190 0.78341623 0.32409705 175.26282770  
0.58634766 0.06279337 0.80762207 67.66774149  
0.61235309 0.61831707 -0.49265372 63.78303703  
Axis -0.47660457 -0.72572909 -0.49615055  
Axis point 66.63683922 -0.00000000 15.46103341  
Rotation angle (degrees) 168.54504031  
Shift along axis -164.28550219  
  

> fitmap #1 inMap #2

Fit molecule 5zf6.pdb (#1) to map cryosparc_P1_J138_004_volume_map_sharp.mrc
(#2) using 8555 atoms  
average map value = 0.1101, steps = 452  
shifted from previous position = 0.103  
rotated from previous position = 0.228 degrees  
atoms outside contour = 4873, contour level = 0.0941  
  
Position of 5zf6.pdb (#1) relative to
cryosparc_P1_J138_004_volume_map_sharp.mrc (#2) coordinates:  
Matrix rotation and translation  
-0.53023999 0.78337185 0.32430556 175.25600756  
0.58888164 0.06509041 0.80559397 67.48261123  
0.60997046 0.61813573 -0.49582684 63.99489971  
Axis -0.47680398 -0.72659468 -0.49468993  
Axis point 66.66524331 0.00000000 15.64651192  
Rotation angle (degrees) 168.66308535  
Shift along axis -164.25290123  
  
Average map value = 0.1101 for 8555 atoms, 4873 outside contour  

> fitmap #1 inMap #2

Fit molecule 5zf6.pdb (#1) to map cryosparc_P1_J138_004_volume_map_sharp.mrc
(#2) using 8555 atoms  
average map value = 0.1101, steps = 468  
shifted from previous position = 0.0215  
rotated from previous position = 0.0236 degrees  
atoms outside contour = 4871, contour level = 0.0941  
  
Position of 5zf6.pdb (#1) relative to
cryosparc_P1_J138_004_volume_map_sharp.mrc (#2) coordinates:  
Matrix rotation and translation  
-0.53022549 0.78333267 0.32442389 175.26120378  
0.58869596 0.06476843 0.80575562 67.51017249  
0.61016227 0.61821920 -0.49548665 63.97828856  
Axis -0.47680497 -0.72648012 -0.49485721  
Axis point 66.65954664 0.00000000 15.62105020  
Rotation angle (degrees) 168.65831877  
Shift along axis -164.27032823  
  

> ui mousemode right "rotate selected models"

> ui mousemode right "translate selected models"

> view matrix models
> #3,-0.75983,0.62964,-0.16191,211.35,-0.13348,-0.39483,-0.90901,195.66,-0.63628,-0.66908,0.38404,229.11

> ui mousemode right "rotate selected models"

> view matrix models
> #3,-0.90104,0.41237,0.13447,215.67,-0.29508,-0.35557,-0.88685,207.87,-0.31789,-0.83876,0.44206,202.71

> fitmap #1 inMap #2

Fit molecule 5zf6.pdb (#1) to map cryosparc_P1_J138_004_volume_map_sharp.mrc
(#2) using 8555 atoms  
average map value = 0.1101, steps = 1032  
shifted from previous position = 0.0229  
rotated from previous position = 0.0182 degrees  
atoms outside contour = 4867, contour level = 0.0941  
  
Position of 5zf6.pdb (#1) relative to
cryosparc_P1_J138_004_volume_map_sharp.mrc (#2) coordinates:  
Matrix rotation and translation  
-0.53018707 0.78332483 0.32450560 175.24771623  
0.58892855 0.06488799 0.80557601 67.47459733  
0.60997117 0.61821660 -0.49572513 63.99835587  
Axis -0.47684177 -0.72652827 -0.49475104  
Axis point 66.65970814 0.00000000 15.64161582  
Rotation angle (degrees) 168.67005183  
Shift along axis -164.25088775  
  

> fitmap #1 inMap #2

Fit molecule 5zf6.pdb (#1) to map cryosparc_P1_J138_004_volume_map_sharp.mrc
(#2) using 8555 atoms  
average map value = 0.1101, steps = 1664  
shifted from previous position = 0.0186  
rotated from previous position = 0.0171 degrees  
atoms outside contour = 4870, contour level = 0.0941  
  
Position of 5zf6.pdb (#1) relative to
cryosparc_P1_J138_004_volume_map_sharp.mrc (#2) coordinates:  
Matrix rotation and translation  
-0.53022877 0.78335427 0.32436637 175.26165080  
0.58869921 0.06483364 0.80574800 67.50564141  
0.61015628 0.61818500 -0.49553670 63.97927276  
Axis -0.47680080 -0.72650180 -0.49482939  
Axis point 66.66175547 -0.00000000 15.62607931  
Rotation angle (degrees) 168.65658943  
Shift along axis -164.26669023  
  

> fitmap #1 inMap #2

Fit molecule 5zf6.pdb (#1) to map cryosparc_P1_J138_004_volume_map_sharp.mrc
(#2) using 8555 atoms  
average map value = 0.1101, steps = 644  
shifted from previous position = 0.00609  
rotated from previous position = 0.0107 degrees  
atoms outside contour = 4869, contour level = 0.0941  
  
Position of 5zf6.pdb (#1) relative to
cryosparc_P1_J138_004_volume_map_sharp.mrc (#2) coordinates:  
Matrix rotation and translation  
-0.53021367 0.78337477 0.32434154 175.25632282  
0.58880640 0.06495516 0.80565988 67.49569086  
0.61006597 0.61814626 -0.49569620 63.99027421  
Axis -0.47681347 -0.72654591 -0.49475242  
Axis point 66.66093857 0.00000000 15.63720472  
Rotation angle (degrees) 168.65992061  
Shift along axis -164.26263689  
  

> fitmap #1 inMap #2

Fit molecule 5zf6.pdb (#1) to map cryosparc_P1_J138_004_volume_map_sharp.mrc
(#2) using 8555 atoms  
average map value = 0.1092, steps = 2000  
shifted from previous position = 0.317  
rotated from previous position = 0.649 degrees  
atoms outside contour = 4884, contour level = 0.0941  
  
Position of 5zf6.pdb (#1) relative to
cryosparc_P1_J138_004_volume_map_sharp.mrc (#2) coordinates:  
Matrix rotation and translation  
-0.53069682 0.78334548 0.32362130 175.24667359  
0.59519461 0.07260582 0.80029480 67.09333677  
0.60341053 0.61733156 -0.50477469 64.56830683  
Axis -0.47694847 -0.72935438 -0.49047156  
Axis point 66.74526995 0.00000000 16.15781383  
Rotation angle (degrees) 168.94179545  
Shift along axis -164.18736942  
  

> fitmap #1 inMap #2

Fit molecule 5zf6.pdb (#1) to map cryosparc_P1_J138_004_volume_map_sharp.mrc
(#2) using 8555 atoms  
average map value = 0.1092, steps = 2000  
shifted from previous position = 0.49  
rotated from previous position = 0.92 degrees  
atoms outside contour = 4936, contour level = 0.0941  
  
Position of 5zf6.pdb (#1) relative to
cryosparc_P1_J138_004_volume_map_sharp.mrc (#2) coordinates:  
Matrix rotation and translation  
-0.53116199 0.78451417 0.32000698 175.45737218  
0.58405988 0.06541926 0.80907007 68.06310467  
0.61379231 0.61665050 -0.49295147 63.69301951  
Axis -0.47584743 -0.72652173 -0.49571704  
Axis point 66.69465830 0.00000000 15.45750434  
Rotation angle (degrees) 168.33516879  
Shift along axis -164.51398071  
  

> ui tool show "Fit in Map"

> view matrix models
> #3,-0.82929,0.50215,0.2452,204.87,-0.51079,-0.50318,-0.69707,221.16,-0.22665,-0.70332,0.67378,185.65

> volume #2 level 0.1033

> color #3 #e17cd6ff

> color #3 #e15cdaff

> view matrix models
> #3,-0.31693,0.8998,0.29986,155.65,-0.87959,-0.39711,0.26196,218.08,0.35479,-0.18073,0.91731,122.78

> view matrix models
> #3,-0.42234,0.64566,0.6362,156.08,-0.74681,0.14992,-0.64792,231.27,-0.51371,-0.74876,0.41887,218.7

> view matrix models
> #3,-0.43035,0.65719,0.61879,157.19,-0.61572,0.28756,-0.73362,221.62,-0.66006,-0.69672,0.28089,234.91

> view matrix models
> #3,-0.43231,0.66215,0.6121,157.52,-0.55808,0.33671,-0.7584,217.08,-0.70827,-0.66947,0.22397,240.52

> ui mousemode right "translate selected models"

> view matrix models
> #3,-0.43231,0.66215,0.6121,160.72,-0.55808,0.33671,-0.7584,219.01,-0.70827,-0.66947,0.22397,239.83

> view matrix models
> #3,-0.43231,0.66215,0.6121,159.28,-0.55808,0.33671,-0.7584,218.31,-0.70827,-0.66947,0.22397,240.9

> fitmap #1 inMap #2

Fit molecule 5zf6.pdb (#1) to map cryosparc_P1_J138_004_volume_map_sharp.mrc
(#2) using 8555 atoms  
average map value = 0.1097, steps = 2000  
shifted from previous position = 0.352  
rotated from previous position = 0.512 degrees  
atoms outside contour = 5164, contour level = 0.10328  
  
Position of 5zf6.pdb (#1) relative to
cryosparc_P1_J138_004_volume_map_sharp.mrc (#2) coordinates:  
Matrix rotation and translation  
-0.52938973 0.78232018 0.32820976 175.20180548  
0.58937752 0.06086670 0.80556153 67.55099551  
0.61023000 0.61989546 -0.49330413 63.76830767  
Axis -0.47742766 -0.72519584 -0.49613891  
Axis point 66.56518497 0.00000000 15.32623750  
Rotation angle (degrees) 168.78774324  
Shift along axis -164.27182856  
  

> view matrix models
> #3,-0.43231,0.66215,0.6121,149.22,-0.55808,0.33671,-0.7584,293.13,-0.70827,-0.66947,0.22397,224.36

> ui mousemode right "rotate selected models"

> view matrix models
> #3,0.32146,-0.80003,-0.50657,141.97,0.63696,-0.21316,0.74085,149.61,-0.70068,-0.56081,0.44107,215.08

> ui mousemode right "move picked models"

> view matrix models
> #3,0.32146,-0.80003,-0.50657,152.54,0.63696,-0.21316,0.74085,70.881,-0.70068,-0.56081,0.44107,232.12

> view matrix models #2,1,0,0,0.49818,0,1,0,-1.9443,0,0,1,-0.027411

> view matrix models
> #3,0.32146,-0.80003,-0.50657,152.17,0.63696,-0.21316,0.74085,71.821,-0.70068,-0.56081,0.44107,231.13

> fitmap #1 inMap #2

Fit molecule 5zf6.pdb (#1) to map cryosparc_P1_J138_004_volume_map_sharp.mrc
(#2) using 8555 atoms  
average map value = 0.1101, steps = 444  
shifted from previous position = 2.05  
rotated from previous position = 0.272 degrees  
atoms outside contour = 5177, contour level = 0.10328  
  
Position of 5zf6.pdb (#1) relative to
cryosparc_P1_J138_004_volume_map_sharp.mrc (#2) coordinates:  
Matrix rotation and translation  
-0.53014537 0.78341422 0.32435791 175.24973781  
0.58901723 0.06509488 0.80549448 67.46874039  
0.60992178 0.61808157 -0.49595422 64.01644841  
Axis -0.47685986 -0.72659856 -0.49463037  
Axis point 66.66173550 0.00000000 15.66125669  
Rotation angle (degrees) 168.66720919  
Shift along axis -164.25673514  
  

> fitmap #1 inMap #2

Fit molecule 5zf6.pdb (#1) to map cryosparc_P1_J138_004_volume_map_sharp.mrc
(#2) using 8555 atoms  
average map value = 0.109, steps = 2000  
shifted from previous position = 0.364  
rotated from previous position = 0.268 degrees  
atoms outside contour = 5184, contour level = 0.10328  
  
Position of 5zf6.pdb (#1) relative to
cryosparc_P1_J138_004_volume_map_sharp.mrc (#2) coordinates:  
Matrix rotation and translation  
-0.52953165 0.78373063 0.32459593 175.22933259  
0.58649074 0.06178533 0.80759593 67.65591347  
0.61288240 0.61802012 -0.49236805 64.00402190  
Axis -0.47700270 -0.72537431 -0.49628674  
Axis point 66.58693830 0.00000000 15.55380403  
Rotation angle (degrees) 168.53813576  
Shift along axis -164.42507401  
  

> hide #!1 models

> fitmap #1 inMap #2

Fit molecule 5zf6.pdb (#1) to map cryosparc_P1_J138_004_volume_map_sharp.mrc
(#2) using 8555 atoms  
average map value = 0.1101, steps = 880  
shifted from previous position = 0.36  
rotated from previous position = 0.25 degrees  
atoms outside contour = 5168, contour level = 0.10328  
  
Position of 5zf6.pdb (#1) relative to
cryosparc_P1_J138_004_volume_map_sharp.mrc (#2) coordinates:  
Matrix rotation and translation  
-0.53016490 0.78326941 0.32467554 175.24946771  
0.58893795 0.06470422 0.80558392 67.47703695  
0.60998137 0.61830606 -0.49560099 63.99897669  
Axis -0.47686117 -0.72646748 -0.49482160  
Axis point 66.65753160 0.00000000 15.63292089  
Rotation angle (degrees) 168.67551546  
Shift along axis -164.25761625  
  

> fitmap #1 inMap #2

Fit molecule 5zf6.pdb (#1) to map cryosparc_P1_J138_004_volume_map_sharp.mrc
(#2) using 8555 atoms  
average map value = 0.1101, steps = 956  
shifted from previous position = 0.00538  
rotated from previous position = 0.0193 degrees  
atoms outside contour = 5176, contour level = 0.10328  
  
Position of 5zf6.pdb (#1) relative to
cryosparc_P1_J138_004_volume_map_sharp.mrc (#2) coordinates:  
Matrix rotation and translation  
-0.53015255 0.78339221 0.32439933 175.25212537  
0.58891863 0.06495837 0.80557759 67.47860241  
0.61001075 0.61812382 -0.49579211 64.00549538  
Axis -0.47685229 -0.72654962 -0.49470956  
Axis point 66.66066154 0.00000000 15.64936302  
Rotation angle (degrees) 168.66452359  
Shift along axis -164.26006100  
  

> fitmap #1 inMap #2

Fit molecule 5zf6.pdb (#1) to map cryosparc_P1_J138_004_volume_map_sharp.mrc
(#2) using 8555 atoms  
average map value = 0.1101, steps = 112  
shifted from previous position = 0.00517  
rotated from previous position = 0.00997 degrees  
atoms outside contour = 5171, contour level = 0.10328  
  
Position of 5zf6.pdb (#1) relative to
cryosparc_P1_J138_004_volume_map_sharp.mrc (#2) coordinates:  
Matrix rotation and translation  
-0.53016501 0.78334523 0.32449237 175.25164793  
0.58886816 0.06482196 0.80562547 67.48756934  
0.61004864 0.61819767 -0.49565340 63.99723866  
Axis -0.47684777 -0.72650305 -0.49478230  
Axis point 66.65766971 0.00000000 15.63680662  
Rotation angle (degrees) 168.66600557  
Shift along axis -164.26298379  
  

> view matrix models #2,1,0,0,-2.1044,0,1,0,-5.9865,0,0,1,-10.395

> view matrix models
> #3,0.32146,-0.80003,-0.50657,166.64,0.63696,-0.21316,0.74085,116.98,-0.70068,-0.56081,0.44107,217.96

> view matrix models
> #3,0.32146,-0.80003,-0.50657,169.54,0.63696,-0.21316,0.74085,125.06,-0.70068,-0.56081,0.44107,218.42

> open /Users/madhurikanavalli/Desktop/ImageProcessing/5zf6.pdb

5zf6.pdb title:  
Crystal structure of the dimeric human pnpase [more info...]  
  
Chain information for 5zf6.pdb #4  
---  
Chain | Description | UniProt  
A B | polyribonucleotide nucleotidyltransferase 1, mitochondrial | PNPT1_HUMAN 46-669  
  

> view matrix models
> #3,0.32146,-0.80003,-0.50657,148.43,0.63696,-0.21316,0.74085,63.647,-0.70068,-0.56081,0.44107,223.56

> view matrix models #2,1,0,0,48.511,0,1,0,45.019,0,0,1,20.98

> select subtract #3

Nothing selected  

> select add #4

8555 atoms, 8618 bonds, 1174 residues, 1 model selected  

> view matrix models #4,1,0,0,83.657,0,1,0,133.4,0,0,1,74.498

> view matrix models #2,1,0,0,52.3,0,1,0,49.451,0,0,1,28.52

> select add #3

17110 atoms, 17236 bonds, 2348 residues, 2 models selected  

> select subtract #4

8555 atoms, 8618 bonds, 1174 residues, 1 model selected  

> view matrix models
> #3,0.32146,-0.80003,-0.50657,136.64,0.63696,-0.21316,0.74085,-37.639,-0.70068,-0.56081,0.44107,316.42

> select add #4

17110 atoms, 17236 bonds, 2348 residues, 2 models selected  

> select subtract #3

8555 atoms, 8618 bonds, 1174 residues, 1 model selected  

> view matrix models #4,1,0,0,100.21,0,1,0,119.24,0,0,1,167.97

> view matrix models #4,1,0,0,94.426,0,1,0,100.65,0,0,1,171.22

> ui mousemode right "rotate selected models"

> view matrix models
> #4,0.41408,0.1075,0.90387,111.26,-0.67576,0.70158,0.22613,152.77,-0.60983,-0.70443,0.36315,251.95

> view matrix models
> #4,-0.63644,0.71625,0.28624,212.21,-0.65653,-0.30824,-0.68845,194.26,-0.40487,-0.62608,0.66641,223.7

> ui mousemode right "translate selected models"

> view matrix models
> #4,-0.63644,0.71625,0.28624,224.74,-0.65653,-0.30824,-0.68845,278.34,-0.40487,-0.62608,0.66641,230.36

> fitmap #1 inMap #2

Fit molecule 5zf6.pdb (#1) to map cryosparc_P1_J138_004_volume_map_sharp.mrc
(#2) using 8555 atoms  
average map value = 0.005866, steps = 116  
shifted from previous position = 9.15  
rotated from previous position = 11.8 degrees  
atoms outside contour = 8423, contour level = 0.10328  
  
Position of 5zf6.pdb (#1) relative to
cryosparc_P1_J138_004_volume_map_sharp.mrc (#2) coordinates:  
Matrix rotation and translation  
-0.37376363 0.83073250 0.41253395 113.00914844  
0.60330567 -0.12008129 0.78841788 24.18980167  
0.70450196 0.54356600 -0.45630361 26.46495607  
Axis -0.55176955 -0.65794495 -0.51250250  
Axis point 48.12014517 0.00000000 9.13310885  
Rotation angle (degrees) 167.18059467  
Shift along axis -91.83392043  
  

> fitmap #1 inMap #2

Fit molecule 5zf6.pdb (#1) to map cryosparc_P1_J138_004_volume_map_sharp.mrc
(#2) using 8555 atoms  
average map value = 0.005866, steps = 48  
shifted from previous position = 0.00372  
rotated from previous position = 0.00364 degrees  
atoms outside contour = 8423, contour level = 0.10328  
  
Position of 5zf6.pdb (#1) relative to
cryosparc_P1_J138_004_volume_map_sharp.mrc (#2) coordinates:  
Matrix rotation and translation  
-0.37380897 0.83073280 0.41249227 113.01657042  
0.60329402 -0.12002284 0.78843569 24.18865946  
0.70448788 0.54357845 -0.45631052 26.46914319  
Axis -0.55174775 -0.65796676 -0.51249795  
Axis point 48.12488148 0.00000000 9.13684441  
Rotation angle (degrees) 167.17979429  
Shift along axis -91.83735467  
  

> fitmap #1 inMap #2

Fit molecule 5zf6.pdb (#1) to map cryosparc_P1_J138_004_volume_map_sharp.mrc
(#2) using 8555 atoms  
average map value = 0.005864, steps = 40  
shifted from previous position = 0.0173  
rotated from previous position = 0.0226 degrees  
atoms outside contour = 8423, contour level = 0.10328  
  
Position of 5zf6.pdb (#1) relative to
cryosparc_P1_J138_004_volume_map_sharp.mrc (#2) coordinates:  
Matrix rotation and translation  
-0.37355182 0.83072501 0.41274083 112.98007958  
0.60330989 -0.12040454 0.78836535 24.20207343  
0.70461068 0.54350594 -0.45620727 26.44217135  
Axis -0.55186913 -0.65782221 -0.51255283  
Axis point 48.09869868 0.00000000 9.11163823  
Rotation angle (degrees) 167.18254641  
Shift along axis -91.82388905  
  

> view matrix models
> #4,-0.63644,0.71625,0.28624,225.3,-0.65653,-0.30824,-0.68845,281.25,-0.40487,-0.62608,0.66641,228.09

> view matrix models
> #4,-0.63644,0.71625,0.28624,236.94,-0.65653,-0.30824,-0.68845,284.6,-0.40487,-0.62608,0.66641,231.87

> view matrix models
> #4,-0.63644,0.71625,0.28624,236.34,-0.65653,-0.30824,-0.68845,283.78,-0.40487,-0.62608,0.66641,229.07

> ui mousemode right "rotate selected models"

> view matrix models
> #4,-0.45017,0.42924,0.78301,207.48,-0.89014,-0.14629,-0.43156,292.56,-0.070696,-0.89126,0.44794,211.86

> view matrix models
> #4,0.34045,-0.89658,0.28327,174.58,-0.051672,0.28297,0.95774,170.65,-0.93884,-0.34069,0.050008,290.91

> fitmap #1 inMap #2

Fit molecule 5zf6.pdb (#1) to map cryosparc_P1_J138_004_volume_map_sharp.mrc
(#2) using 8555 atoms  
average map value = 0.005866, steps = 44  
shifted from previous position = 0.0178  
rotated from previous position = 0.0233 degrees  
atoms outside contour = 8423, contour level = 0.10328  
  
Position of 5zf6.pdb (#1) relative to
cryosparc_P1_J138_004_volume_map_sharp.mrc (#2) coordinates:  
Matrix rotation and translation  
-0.37380507 0.83073257 0.41249627 113.01405219  
0.60332324 -0.12000649 0.78841582 24.18430772  
0.70446493 0.54358241 -0.45634124 26.47196139  
Axis -0.55175121 -0.65797420 -0.51248469  
Axis point 48.12483337 0.00000000 9.13955270  
Rotation angle (degrees) 167.18114524  
Shift along axis -91.83476585  
  

> fitmap #1 inMap #2

Fit molecule 5zf6.pdb (#1) to map cryosparc_P1_J138_004_volume_map_sharp.mrc
(#2) using 8555 atoms  
average map value = 0.005865, steps = 44  
shifted from previous position = 0.0162  
rotated from previous position = 0.0104 degrees  
atoms outside contour = 8423, contour level = 0.10328  
  
Position of 5zf6.pdb (#1) relative to
cryosparc_P1_J138_004_volume_map_sharp.mrc (#2) coordinates:  
Matrix rotation and translation  
-0.37367588 0.83073709 0.41260419 112.99497364  
0.60333702 -0.12017770 0.78837920 24.19299418  
0.70452167 0.54353768 -0.45630693 26.45239851  
Axis -0.55181172 -0.65790916 -0.51250304  
Axis point 48.11098906 0.00000000 9.12355336  
Rotation angle (degrees) 167.18214242  
Shift along axis -91.82567741  
  

> view matrix models
> #4,0.43664,-0.89777,0.05801,174.22,0.24953,0.18281,0.95095,147.04,-0.86434,-0.40075,0.30385,276.84

> ui mousemode right "translate selected models"

> view matrix models
> #4,0.43664,-0.89777,0.05801,174.07,0.24953,0.18281,0.95095,141.74,-0.86434,-0.40075,0.30385,275.58

> fitmap #1 inMap #2

Fit molecule 5zf6.pdb (#1) to map cryosparc_P1_J138_004_volume_map_sharp.mrc
(#2) using 8555 atoms  
average map value = 0.005865, steps = 64  
shifted from previous position = 0.000311  
rotated from previous position = 0.00048 degrees  
atoms outside contour = 8423, contour level = 0.10328  
  
Position of 5zf6.pdb (#1) relative to
cryosparc_P1_J138_004_volume_map_sharp.mrc (#2) coordinates:  
Matrix rotation and translation  
-0.37368007 0.83073787 0.41259884 112.99539390  
0.60333786 -0.12016939 0.78837982 24.19256137  
0.70451872 0.54353833 -0.45631070 26.45291856  
Axis -0.55180974 -0.65791232 -0.51250111  
Axis point 48.11140469 0.00000000 9.12410653  
Rotation angle (degrees) 167.18209740  
Shift along axis -91.82569332  
  

> ui tool show "Fit in Map"

> select add #1

17110 atoms, 17236 bonds, 2348 residues, 2 models selected  

> show #!1 models

> view matrix models
> #1,-0.37368,0.83074,0.4126,173.74,0.60334,-0.12017,0.78838,115.48,0.70452,0.54354,-0.45631,93.202,#4,0.43664,-0.89777,0.05801,182.52,0.24953,0.18281,0.95095,183.58,-0.86434,-0.40075,0.30385,313.81

> select subtract #1

8555 atoms, 8618 bonds, 1174 residues, 1 model selected  

> fitmap #1 inMap #2

Fit molecule 5zf6.pdb (#1) to map cryosparc_P1_J138_004_volume_map_sharp.mrc
(#2) using 8555 atoms  
average map value = 0.04826, steps = 208  
shifted from previous position = 24.3  
rotated from previous position = 13.9 degrees  
atoms outside contour = 7062, contour level = 0.10328  
  
Position of 5zf6.pdb (#1) relative to
cryosparc_P1_J138_004_volume_map_sharp.mrc (#2) coordinates:  
Matrix rotation and translation  
-0.53783333 0.72307022 0.43347983 159.00633082  
0.47779001 -0.16220016 0.86337003 75.07630856  
0.69458766 0.67146152 -0.25823907 50.90548584  
Axis -0.47220498 -0.64247495 -0.60352994  
Axis point 55.27447343 0.00000000 -2.91680074  
Rotation angle (degrees) 168.27557772  
Shift along axis -154.04121419  
  

> view matrix models
> #4,0.43664,-0.89777,0.05801,191.01,0.24953,0.18281,0.95095,146.47,-0.86434,-0.40075,0.30385,280.45

> fitmap #1 inMap #2

Fit molecule 5zf6.pdb (#1) to map cryosparc_P1_J138_004_volume_map_sharp.mrc
(#2) using 8555 atoms  
average map value = 0.04826, steps = 44  
shifted from previous position = 0.00861  
rotated from previous position = 0.0257 degrees  
atoms outside contour = 7058, contour level = 0.10328  
  
Position of 5zf6.pdb (#1) relative to
cryosparc_P1_J138_004_volume_map_sharp.mrc (#2) coordinates:  
Matrix rotation and translation  
-0.53794322 0.72292001 0.43359400 159.01744152  
0.47809988 -0.16198800 0.86323832 75.04555828  
0.69428928 0.67167443 -0.25848763 50.93543074  
Axis -0.47217660 -0.64257525 -0.60344536  
Axis point 55.28749168 -0.00000000 -2.88908802  
Rotation angle (degrees) 168.29621721  
Shift along axis -154.04348260  
  

> fitmap #1 inMap #2

Fit molecule 5zf6.pdb (#1) to map cryosparc_P1_J138_004_volume_map_sharp.mrc
(#2) using 8555 atoms  
average map value = 0.04827, steps = 44  
shifted from previous position = 0.0189  
rotated from previous position = 0.0343 degrees  
atoms outside contour = 7055, contour level = 0.10328  
  
Position of 5zf6.pdb (#1) relative to
cryosparc_P1_J138_004_volume_map_sharp.mrc (#2) coordinates:  
Matrix rotation and translation  
-0.53786107 0.72305980 0.43346279 159.01065126  
0.47765769 -0.16229540 0.86342535 75.09640503  
0.69465717 0.67144972 -0.25808270 50.89975282  
Axis -0.47218320 -0.64243364 -0.60359096  
Axis point 55.27191933 0.00000000 -2.93139955  
Rotation angle (degrees) 168.27087076  
Shift along axis -154.04924535  
  

> fitmap #1 inMap #2

Fit molecule 5zf6.pdb (#1) to map cryosparc_P1_J138_004_volume_map_sharp.mrc
(#2) using 8555 atoms  
average map value = 0.04826, steps = 56  
shifted from previous position = 0.00275  
rotated from previous position = 0.00863 degrees  
atoms outside contour = 7058, contour level = 0.10328  
  
Position of 5zf6.pdb (#1) relative to
cryosparc_P1_J138_004_volume_map_sharp.mrc (#2) coordinates:  
Matrix rotation and translation  
-0.53789045 0.72301563 0.43350002 159.01318424  
0.47776580 -0.16222232 0.86337927 75.08570285  
0.69456008 0.67151495 -0.25817430 50.90982184  
Axis -0.47217744 -0.64246791 -0.60355899  
Axis point 55.27562048 0.00000000 -2.92169624  
Rotation angle (degrees) 168.27762248  
Shift along axis -154.04967314  
  

> fitmap #1 inMap #2

Fit molecule 5zf6.pdb (#1) to map cryosparc_P1_J138_004_volume_map_sharp.mrc
(#2) using 8555 atoms  
average map value = 0.04827, steps = 48  
shifted from previous position = 0.00193  
rotated from previous position = 0.00615 degrees  
atoms outside contour = 7058, contour level = 0.10328  
  
Position of 5zf6.pdb (#1) relative to
cryosparc_P1_J138_004_volume_map_sharp.mrc (#2) coordinates:  
Matrix rotation and translation  
-0.53789039 0.72302397 0.43348618 159.01244545  
0.47768494 -0.16228399 0.86341242 75.09257148  
0.69461574 0.67149106 -0.25808666 50.90376590  
Axis -0.47217206 -0.64244065 -0.60359221  
Axis point 55.27392700 -0.00000000 -2.92888590  
Rotation angle (degrees) 168.27395306  
Shift along axis -154.04887104  
  

> fitmap #1 inMap #2

Fit molecule 5zf6.pdb (#1) to map cryosparc_P1_J138_004_volume_map_sharp.mrc
(#2) using 8555 atoms  
average map value = 0.04827, steps = 64  
shifted from previous position = 0.00548  
rotated from previous position = 0.00426 degrees  
atoms outside contour = 7055, contour level = 0.10328  
  
Position of 5zf6.pdb (#1) relative to
cryosparc_P1_J138_004_volume_map_sharp.mrc (#2) coordinates:  
Matrix rotation and translation  
-0.53785442 0.72306533 0.43346182 159.01147157  
0.47764879 -0.16230580 0.86342832 75.10035018  
0.69466845 0.67144125 -0.25807439 50.90069383  
Axis -0.47218581 -0.64242903 -0.60359383  
Axis point 55.27087759 0.00000000 -2.93287966  
Rotation angle (degrees) 168.27022770  
Shift along axis -154.05294973  
  

> fitmap #1 inMap #2

Fit molecule 5zf6.pdb (#1) to map cryosparc_P1_J138_004_volume_map_sharp.mrc
(#2) using 8555 atoms  
average map value = 0.04827, steps = 60  
shifted from previous position = 0.00642  
rotated from previous position = 0.00609 degrees  
atoms outside contour = 7058, contour level = 0.10328  
  
Position of 5zf6.pdb (#1) relative to
cryosparc_P1_J138_004_volume_map_sharp.mrc (#2) coordinates:  
Matrix rotation and translation  
-0.53785882 0.72305154 0.43347937 159.00964336  
0.47772910 -0.16224717 0.86339490 75.08872707  
0.69460982 0.67147027 -0.25815669 50.90531403  
Axis -0.47218929 -0.64245532 -0.60356311  
Axis point 55.27291232 0.00000000 -2.92451182  
Rotation angle (degrees) 168.27418388  
Shift along axis -154.04837304  
  

> fitmap #1 inMap #2

Fit molecule 5zf6.pdb (#1) to map cryosparc_P1_J138_004_volume_map_sharp.mrc
(#2) using 8555 atoms  
average map value = 0.04826, steps = 64  
shifted from previous position = 0.0135  
rotated from previous position = 0.016 degrees  
atoms outside contour = 7058, contour level = 0.10328  
  
Position of 5zf6.pdb (#1) relative to
cryosparc_P1_J138_004_volume_map_sharp.mrc (#2) coordinates:  
Matrix rotation and translation  
-0.53787316 0.72300698 0.43353588 159.01006466  
0.47794254 -0.16210192 0.86330406 75.06071540  
0.69445186 0.67155333 -0.25836552 50.91864025  
Axis -0.47219782 -0.64252143 -0.60348607  
Axis point 55.27987228 0.00000000 -2.90357183  
Rotation angle (degrees) 168.28517374  
Shift along axis -154.04101345  
  

> view matrix models
> #4,0.43664,-0.89777,0.05801,173.3,0.24953,0.18281,0.95095,144.52,-0.86434,-0.40075,0.30385,283.13

> select add #1

17110 atoms, 17236 bonds, 2348 residues, 2 models selected  

> select subtract #4

8555 atoms, 8618 bonds, 1174 residues, 1 model selected  

> view matrix models
> #1,-0.53787,0.72301,0.43354,223.6,0.47794,-0.1621,0.8633,124.88,0.69445,0.67155,-0.25837,77.874

> fitmap #1 inMap #2

Fit molecule 5zf6.pdb (#1) to map cryosparc_P1_J138_004_volume_map_sharp.mrc
(#2) using 8555 atoms  
average map value = 0.1101, steps = 1948  
shifted from previous position = 2.6  
rotated from previous position = 15.7 degrees  
atoms outside contour = 5173, contour level = 0.10328  
  
Position of 5zf6.pdb (#1) relative to
cryosparc_P1_J138_004_volume_map_sharp.mrc (#2) coordinates:  
Matrix rotation and translation  
-0.53017143 0.78335704 0.32445337 175.25064083  
0.58878998 0.06478821 0.80568533 67.49213641  
0.61011852 0.61818624 -0.49558164 63.99599175  
Axis -0.47683791 -0.72648873 -0.49481283  
Axis point 66.65667254 0.00000000 15.63558830  
Rotation angle (degrees) 168.66140010  
Shift along axis -164.26446361  
  

> view matrix models
> #1,-0.53017,0.78336,0.32445,226.92,0.58879,0.064788,0.80569,120.46,0.61012,0.61819,-0.49558,88.341

> fitmap #1 inMap #2

Fit molecule 5zf6.pdb (#1) to map cryosparc_P1_J138_004_volume_map_sharp.mrc
(#2) using 8555 atoms  
average map value = 0.0603, steps = 72  
shifted from previous position = 1.36  
rotated from previous position = 2.65 degrees  
atoms outside contour = 6643, contour level = 0.10328  
  
Position of 5zf6.pdb (#1) relative to
cryosparc_P1_J138_004_volume_map_sharp.mrc (#2) coordinates:  
Matrix rotation and translation  
-0.56226080 0.77257068 0.29495311 179.14945505  
0.55854681 0.09176525 0.82438134 73.22777991  
0.60982640 0.62826243 -0.48311289 60.26848266  
Axis -0.45793431 -0.73522379 -0.49974208  
Axis point 68.44102626 0.00000000 12.84484093  
Rotation angle (degrees) 167.63525506  
Shift along axis -165.99618459  
  

[deleted to fit within ticket limits]

> fitmap #7 inMap #2

Fit molecule AF-Q8TCS8-F1-model_v6.pdb (#7) to map
cryosparc_P1_J482_004_volume_map.mrc (#2) using 6032 atoms  
average map value = -5.856e-05, steps = 368  
shifted from previous position = 2.09  
rotated from previous position = 5.84 degrees  
atoms outside contour = 6032, contour level = 0.074721  
  
Position of AF-Q8TCS8-F1-model_v6.pdb (#7) relative to
cryosparc_P1_J482_004_volume_map.mrc (#2) coordinates:  
Matrix rotation and translation  
0.08710074 -0.92938392 0.35869061 59.59075313  
-0.79201574 -0.28300142 -0.54094475 16.28119012  
0.60425532 -0.23697192 -0.76073634 160.17750188  
Axis 0.73385821 -0.59284799 0.33163743  
Axis point 0.00000000 22.84851283 71.47256607  
Rotation angle (degrees) 168.04719174  
Shift along axis 87.19974831  
  

> view matrix models
> #7,-0.17868,-0.97881,-0.10005,6.648,-0.7608,0.20193,-0.61677,36.832,0.62391,-0.034088,-0.78076,112.96

> view matrix models
> #7,-0.20095,-0.96996,0.13707,14.191,-0.86316,0.10916,-0.493,40.09,0.46323,-0.21738,-0.85917,109.21

> view matrix models
> #7,-0.10038,-0.9943,0.035932,11.249,-0.86855,0.069951,-0.49065,40.01,0.48534,-0.080459,-0.87062,109.4

> ui mousemode right "move picked models"

> view matrix models
> #7,-0.10038,-0.9943,0.035932,7.572,-0.86855,0.069951,-0.49065,29.368,0.48534,-0.080459,-0.87062,77.9

> view matrix models
> #2,0.85656,0.43699,0.27448,-91.905,-0.45132,0.89228,-0.012127,31.709,-0.25021,-0.11349,0.96152,-24.5

> view matrix models
> #7,0.10537,-0.99398,0.030033,12.071,-0.94351,-0.10947,-0.31273,46.098,0.31413,0.0046163,-0.94937,108.85

> view matrix models
> #7,-0.56765,-0.71499,-0.40811,-7.3912,-0.38128,0.66768,-0.6394,71.402,0.72966,-0.20735,-0.65162,110.98

> view matrix models
> #2,0.93609,0.26905,0.22662,-77.448,-0.28402,0.95816,0.035632,19.557,-0.20755,-0.097718,0.97333,-57.413

> view matrix models
> #2,0.93609,0.26905,0.22662,-77.709,-0.28402,0.95816,0.035632,24.136,-0.20755,-0.097718,0.97333,-56.529

> view matrix models
> #7,0.039543,-0.98518,0.16692,17.982,-0.87376,-0.11513,-0.47253,24.985,0.48474,-0.12716,-0.86536,114.25

> view matrix models
> #2,0.93609,0.26905,0.22662,-72.357,-0.28402,0.95816,0.035632,7.0311,-0.20755,-0.097718,0.97333,-29.712

> ui mousemode right "rotate selected models"

> view matrix models
> #7,-0.097094,-0.9571,0.27301,20.96,-0.44484,-0.20365,-0.87215,13.504,0.89033,-0.20613,-0.40599,130.24

> view matrix models
> #7,-0.07236,-0.95607,0.28407,21.412,-0.44804,-0.2233,-0.86568,13.632,0.89108,-0.18992,-0.4122,130.1

> ui mousemode right "move picked models"

> view matrix models
> #7,-0.07236,-0.95607,0.28407,15.841,-0.44804,-0.2233,-0.86568,68.667,0.89108,-0.18992,-0.4122,123.93

> view matrix models
> #7,-0.31735,-0.94146,-0.11376,0.63703,-0.20623,0.18561,-0.96074,85.778,0.92561,-0.28142,-0.25306,128.41

> view matrix models
> #7,0.29795,0.52938,-0.79435,-11.389,0.3903,0.69185,0.60746,91.578,0.87114,-0.49103,-0.00047747,123.65

> view matrix models
> #2,-0.0079881,0.99894,0.04544,-17.486,-0.99199,-0.0021879,-0.12629,318.3,-0.12606,-0.046085,0.99095,-56.337

> view matrix models
> #7,-0.1715,0.44788,-0.87749,-17.133,0.40556,0.84381,0.35143,88.21,0.89784,-0.29561,-0.32636,109.47

> view matrix models
> #7,-0.1715,0.44788,-0.87749,-17.408,0.40556,0.84381,0.35143,90.261,0.89784,-0.29561,-0.32636,108.77

> view matrix models
> #7,-0.19986,0.45505,-0.86775,-15.159,0.30662,0.87018,0.38571,82.163,0.93061,-0.18898,-0.31344,118.36

> view matrix models
> #7,-0.19986,0.45505,-0.86775,-19.12,0.30662,0.87018,0.38571,99.076,0.93061,-0.18898,-0.31344,101

> view matrix models
> #7,-0.19986,0.45505,-0.86775,-20.753,0.30662,0.87018,0.38571,106.14,0.93061,-0.18898,-0.31344,93.903

> view matrix models
> #7,-0.19986,0.45505,-0.86775,-14.691,0.30662,0.87018,0.38571,84.809,0.93061,-0.18898,-0.31344,123.13

> view matrix models
> #7,-0.19986,0.45505,-0.86775,-19.112,0.30662,0.87018,0.38571,85.454,0.93061,-0.18898,-0.31344,93.102

> view matrix models
> #7,-0.44672,-0.85339,-0.26864,-7.3842,-0.29981,0.4257,-0.85375,105.34,0.84294,-0.30085,-0.44603,111.24

> close session

> open
> /Users/madhurikanavalli/Desktop/2025/Cryosparc/Q387R/tetramerfit_J482_goodone.pdb
> format pdb

Chain information for tetramerfit_J482_goodone.pdb #1  
---  
Chain | Description  
A C | No description available  
B D | No description available  
  

> view matrix models #1,1,0,0,29.011,0,1,0,-2.0292,0,0,1,-1.0087

> view matrix models #1,1,0,0,23.646,0,1,0,-0.29777,0,0,1,-1.2033

> view matrix models #1,1,0,0,15.181,0,1,0,-4.4582,0,0,1,-0.91014

> view matrix models #1,1,0,0,-15.467,0,1,0,-15.673,0,0,1,15.003

> view matrix models #1,1,0,0,-18.434,0,1,0,-4.2218,0,0,1,-10.466

> view matrix models #1,1,0,0,-39.086,0,1,0,-2.3932,0,0,1,-42.474

> view matrix models #1,1,0,0,-7.3595,0,1,0,2.1169,0,0,1,-20.622

> view matrix models #1,1,0,0,56.725,0,1,0,-22.296,0,0,1,-11.337

> view matrix models #1,1,0,0,58.82,0,1,0,-0.298,0,0,1,12.068

> close session

> open /Users/madhurikanavalli/Downloads/cryosparc_P1_J482_004_volume_map.mrc

Opened cryosparc_P1_J482_004_volume_map.mrc as #1, grid size 352,352,352,
pixel 0.846, shown at level 0.0477, step 2, values float32  

> volume #1 level 0.06529

> volume #1 level 0.09016

> volume #1 level 0.06115

> open /Users/madhurikanavalli/Downloads/AF-Q8TCS8-F1-model_v6.pdb

AF-Q8TCS8-F1-model_v6.pdb title:  
Alphafold monomer V2.0 prediction for polyribonucleotide
nucleotidyltransferase 1, mitochondrial (Q8TCS8) [more info...]  
  
Chain information for AF-Q8TCS8-F1-model_v6.pdb #2  
---  
Chain | Description | UniProt  
A | polyribonucleotide nucleotidyltransferase 1, mitochondrial | PNPT1_HUMAN 1-783  
  
Computing secondary structure  

> tile

2 models tiled  

> select add #2

6032 atoms, 6129 bonds, 783 residues, 1 model selected  

> view matrix models #2,1,0,0,132.15,0,1,0,269.68,0,0,1,174.53

> ui mousemode right "rotate selected models"

> view matrix models
> #2,0.42944,0.66337,0.61279,141.41,0.035656,-0.69047,0.72248,301.34,0.90239,-0.28841,-0.32017,144.99

> transparency 50

> view matrix models
> #2,0.63269,0.58288,0.50986,140.27,0.123,-0.72566,0.67697,300.82,0.76458,-0.3656,-0.53081,138.86

> view matrix models
> #2,0.60082,0.75463,0.26374,132.09,0.39851,-0.56876,0.71952,302.55,0.69297,-0.32719,-0.64245,135.08

> ui mousemode right "translate selected models"

> view matrix models
> #2,0.60082,0.75463,0.26374,148.79,0.39851,-0.56876,0.71952,180.76,0.69297,-0.32719,-0.64245,188.93

> view matrix models
> #2,0.6614,0.18966,0.72565,161.37,0.2615,-0.9651,0.0139,190.89,0.70297,0.18057,-0.68792,178.28

> ui mousemode right "rotate selected models"

> view matrix models
> #2,0.19924,0.94382,-0.26363,126.11,0.83456,-0.022419,0.55046,202.79,0.51363,-0.32969,-0.79215,177.72

> ui mousemode right "translate selected models"

> view matrix models
> #2,0.19924,0.94382,-0.26363,128.38,0.83456,-0.022419,0.55046,186.45,0.51363,-0.32969,-0.79215,184.65

> view matrix models
> #2,0.58266,0.81137,0.046707,142.97,0.47764,-0.38837,0.78805,177.72,0.65754,-0.43686,-0.61384,190.77

> ui mousemode right "rotate selected models"

> view matrix models
> #2,0.57664,0.24616,0.77903,167.09,-0.2162,-0.87356,0.43606,167.17,0.78787,-0.41988,-0.45051,195.95

> view matrix models
> #2,0.76261,0.6462,-0.029199,143.01,0.45171,-0.4997,0.73909,176.95,0.46301,-0.57683,-0.67297,188.94

> ui mousemode right "translate selected models"

> view matrix models
> #2,0.76261,0.6462,-0.029199,142.26,0.45171,-0.4997,0.73909,182.18,0.46301,-0.57683,-0.67297,186.98

> ui mousemode right "rotate selected models"

> ui mousemode right "translate selected models"

> ui mousemode right "rotate selected models"

> view matrix models
> #2,0.8634,0.49418,-0.10162,141.85,0.3123,-0.3653,0.87694,184.3,0.39625,-0.78889,-0.46973,193.67

> ui mousemode right "translate selected models"

> view matrix models
> #2,0.72969,0.68377,0.0039503,132.51,0.33003,-0.35725,0.87376,248.15,0.59886,-0.63627,-0.48634,180.84

> view matrix models
> #2,0.066442,0.43084,-0.89998,103.3,0.93501,0.288,0.2069,257.15,0.34834,-0.85524,-0.3837,150.16

> view matrix models
> #2,0.23351,-0.30901,0.92195,156.19,-0.87131,-0.48737,0.057337,254.89,0.43161,-0.81669,-0.38305,176.46

> ui mousemode right "rotate selected models"

> view matrix models
> #2,0.80143,0.40414,0.44089,141.31,0.16742,-0.85928,0.48333,275.26,0.57418,-0.31354,-0.75631,163.52

> view matrix models
> #2,0.84875,0.50355,0.16142,133.15,0.28808,-0.6963,0.65741,279.7,0.44344,-0.51147,-0.73604,164.66

> view matrix models
> #2,0.86791,0.39835,0.29673,137.73,0.20619,-0.83241,0.51438,276.16,0.45191,-0.38525,-0.80459,161.95

> view matrix models
> #2,0.86632,0.49881,-0.026064,128.07,0.28331,-0.44774,0.84809,283.3,0.41136,-0.74211,-0.5292,171.77

> view matrix models
> #2,0.49235,0.85007,0.18702,129.46,0.42603,-0.42273,0.79988,282.62,0.75901,-0.31414,-0.57028,169.77

> view matrix models
> #2,0.85019,0.48331,0.20878,134.61,0.064393,-0.48905,0.86988,282.92,0.52253,-0.72611,-0.4469,174.6

> ui mousemode right "translate selected models"

> view matrix models
> #2,0.85019,0.48331,0.20878,150.49,0.064393,-0.48905,0.86988,188.61,0.52253,-0.72611,-0.4469,184.74

> view matrix models
> #2,0.64449,0.16707,0.74614,168.48,-0.5504,-0.576,0.60439,164.48,0.53075,-0.80019,-0.27927,193.65

> ui mousemode right "rotate selected models"

> view matrix models
> #2,0.76356,0.63639,0.10946,148.31,0.32497,-0.52519,0.78649,174.28,0.558,-0.56497,-0.60782,183.09

> view matrix models
> #2,0.73909,0.6609,-0.13024,141.34,0.49027,-0.39519,0.77682,174.09,0.46193,-0.63799,-0.6161,182.8

> ui mousemode right "translate selected models"

> view matrix models
> #2,0.73909,0.6609,-0.13024,139.59,0.49027,-0.39519,0.77682,180.83,0.46193,-0.63799,-0.6161,188.6

> ui mousemode right "rotate selected models"

> view matrix models
> #2,0.54319,0.83658,-0.071278,138.91,0.55669,-0.29531,0.77646,180.54,0.62852,-0.46144,-0.62613,188.1

> ui mousemode right "translate selected models"

> view matrix models
> #2,0.75355,0.36167,0.54895,167.57,-0.16644,-0.70289,0.69155,141.22,0.63597,-0.61249,-0.46947,186.49

> view matrix models
> #2,-0.30331,-0.83983,0.45021,143.72,-0.74643,-0.084284,-0.66011,224.32,0.59232,-0.53627,-0.60131,183.9

> view matrix models
> #2,0.58684,-0.34812,0.73105,150.57,-0.47667,-0.87836,-0.035635,264.27,0.65453,-0.32756,-0.6814,180.79

> view matrix models
> #2,0.58684,-0.34812,0.73105,148.38,-0.47667,-0.87836,-0.035635,276.44,0.65453,-0.32756,-0.6814,180.97

> ui mousemode right "rotate selected models"

> view matrix models
> #2,0.63354,0.76624,0.10724,123.81,0.39183,-0.43726,0.80949,302.01,0.66715,-0.47082,-0.57726,184.9

> ui mousemode right "translate selected models"

> view matrix models
> #2,0.63354,0.76624,0.10724,144.96,0.39183,-0.43726,0.80949,181.31,0.66715,-0.47082,-0.57726,189.07

> view matrix models
> #2,0.71133,0.30401,0.63372,164.61,-0.18947,-0.78531,0.5894,164.41,0.67684,-0.53933,-0.50101,194.83

> ui tool show "Fit in Map"

> fitmap #2 inMap #1

Fit molecule AF-Q8TCS8-F1-model_v6.pdb (#2) to map
cryosparc_P1_J482_004_volume_map.mrc (#1) using 6032 atoms  
average map value = 0.04859, steps = 152  
shifted from previous position = 3.28  
rotated from previous position = 5.39 degrees  
atoms outside contour = 4287, contour level = 0.06115  
  
Position of AF-Q8TCS8-F1-model_v6.pdb (#2) relative to
cryosparc_P1_J482_004_volume_map.mrc (#1) coordinates:  
Matrix rotation and translation  
0.56426932 0.76409521 0.31266369 143.04419452  
0.21814827 -0.50324783 0.83615363 170.83785223  
0.79624833 -0.40360881 -0.45065337 194.48298911  
Axis -0.86191870 -0.33620203 -0.37955810  
Axis point 0.00000000 81.61235096 44.75810236  
Rotation angle (degrees) 134.01255267  
Shift along axis -254.54609246  
  

> view matrix models
> #2,0.3492,0.93478,0.065211,136.26,0.66686,-0.2968,0.68352,191.6,0.6583,-0.1952,-0.72701,180.45

> ui mousemode right "rotate selected models"

> view matrix models
> #2,0.89941,-0.31265,0.30546,154.53,-0.35641,-0.92913,0.098419,173.65,0.25305,-0.19739,-0.9471,171.99

> view matrix models
> #2,0.83384,0.55193,-0.008982,139.59,0.4892,-0.73133,0.47523,187.7,0.25573,-0.40066,-0.87981,175.24

> view matrix models
> #2,0.64415,0.75125,0.14382,141.39,0.64873,-0.63619,0.41762,186.39,0.40524,-0.17572,-0.89717,174.12

> ui mousemode right "move picked models"

> view matrix models
> #1,0.87519,0.38044,-0.29884,-2.3734,-0.45145,0.86428,-0.22185,170.72,0.17388,0.32907,0.92816,-69.898

> volume #1 level 0.1131

> volume #1 level 0.07917

> view matrix models
> #2,-0.10638,-0.94919,0.29618,133.6,-0.9431,0.0019433,-0.3325,286.13,0.31503,-0.3147,-0.89539,123

> select #1/A:760-783

Nothing selected  

> select #2/A:760-783

180 atoms, 180 bonds, 24 residues, 1 model selected  

> delete sel

> select #2/1-598

Nothing selected  

> select #2/A:1-598

4596 atoms, 4670 bonds, 598 residues, 1 model selected  

> delete sel

> ui mousemode right "rotate selected models"

> select add #2

1256 atoms, 1277 bonds, 161 residues, 1 model selected  

> view matrix models
> #2,0.6471,0.64962,0.39906,131.95,-0.0059555,-0.5191,0.85469,367.73,0.76238,-0.55545,-0.33205,150

> ui mousemode right "translate selected models"

> view matrix models
> #2,0.6471,0.64962,0.39906,125.43,-0.0059555,-0.5191,0.85469,234.19,0.76238,-0.55545,-0.33205,186.89

> view matrix models
> #2,0.52432,0.60365,0.60057,138.39,0.096076,-0.74273,0.66266,241.7,0.84608,-0.28975,-0.44743,177.45

> view matrix models
> #2,0.58461,0.53787,0.60739,138.89,-0.0034217,-0.74701,0.6648,246.96,0.81131,-0.39073,-0.43487,176.3

> ui mousemode right "move picked models"

> view matrix models
> #1,0.99119,-0.014958,-0.1316,23.999,0.0189,0.99941,0.028756,83.761,0.13109,-0.030989,0.99089,-73.95

> ui mousemode right "translate selected models"

> view matrix models
> #2,0.58461,0.53787,0.60739,147.74,-0.0034217,-0.74701,0.6648,281.09,0.81131,-0.39073,-0.43487,122.35

> fitmap #2 inMap #1

Fit molecule AF-Q8TCS8-F1-model_v6.pdb (#2) to map
cryosparc_P1_J482_004_volume_map.mrc (#1) using 1256 atoms  
average map value = 0.05979, steps = 140  
shifted from previous position = 3.87  
rotated from previous position = 19.7 degrees  
atoms outside contour = 892, contour level = 0.079169  
  
Position of AF-Q8TCS8-F1-model_v6.pdb (#2) relative to
cryosparc_P1_J482_004_volume_map.mrc (#1) coordinates:  
Matrix rotation and translation  
0.69579863 0.56968136 0.43740983 146.46213387  
-0.14760186 -0.48259090 0.86331897 199.16111950  
0.70290675 -0.66525869 -0.25170006 197.09026824  
Axis -0.89429762 -0.15532954 -0.41964808  
Axis point 0.00000000 116.56159785 13.49387603  
Rotation angle (degrees) 121.28170019  
Shift along axis -244.62489409  
  

> view matrix models
> #2,0.64344,0.55251,0.52983,143.2,-0.18754,-0.55726,0.80888,290.18,0.74217,-0.61983,-0.25495,141.57

> view matrix models
> #2,0.31612,0.83453,-0.45126,95.295,0.56826,0.21433,0.79445,277.99,0.75971,-0.50757,-0.40647,132.89

> view matrix models
> #2,-0.86195,-0.25115,-0.44043,128.41,-0.093041,0.93229,-0.34954,173.5,0.49839,-0.2603,-0.82695,98.206

> view matrix models
> #2,0.65233,0.046187,0.75653,156.98,-0.2638,-0.9219,0.28375,231.83,0.71055,-0.38467,-0.5892,137.62

> ui mousemode right "rotate selected models"

> view matrix models
> #2,0.81295,-0.58161,0.028996,122.48,-0.56819,-0.78132,0.25828,234.38,-0.12756,-0.22645,-0.96563,130.95

> view matrix models
> #2,-0.96437,-0.16164,-0.20942,136.53,-0.11386,0.96816,-0.22294,185.23,0.23878,-0.19116,-0.95207,125.06

> ui mousemode right "translate selected models"

> view matrix models
> #2,-0.96437,-0.16164,-0.20942,138.86,-0.11386,0.96816,-0.22294,195.83,0.23878,-0.19116,-0.95207,110.89

> view matrix models
> #2,0.78818,0.14656,0.59774,147.24,-0.054142,-0.95095,0.30456,271.11,0.61306,-0.27241,-0.74159,120.56

> view matrix models
> #2,0.92372,0.30232,0.23524,127.49,-0.038846,0.68486,-0.72764,196.22,-0.38109,0.663,0.64437,183.01

> view matrix models
> #2,0.92372,0.30232,0.23524,121.11,-0.038846,0.68486,-0.72764,201.1,-0.38109,0.663,0.64437,222.65

> ui mousemode right "rotate selected models"

> view matrix models
> #2,-0.13548,-0.69196,-0.70912,99.679,-0.31493,-0.64852,0.69299,291.92,-0.93939,0.31721,-0.13006,195.35

> ui mousemode right "translate selected models"

> view matrix models
> #2,-0.13548,-0.69196,-0.70912,105.56,-0.31493,-0.64852,0.69299,261.53,-0.93939,0.31721,-0.13006,158.07

> view matrix models
> #2,-0.31376,-0.10292,-0.94391,84.999,-0.37062,-0.90198,0.22155,314.04,-0.87418,0.41934,0.24486,213.94

> view matrix models
> #2,0.23037,-0.936,-0.26616,124.75,-0.098671,-0.29457,0.95052,259,-0.96809,-0.19271,-0.16022,160.32

> ui mousemode right "rotate selected models"

> view matrix models
> #2,0.51055,-0.83648,-0.19907,122.5,-0.11184,-0.29416,0.94919,259.15,-0.85254,-0.46235,-0.24374,156.56

> ui mousemode right "translate selected models"

> view matrix models
> #2,0.51055,-0.83648,-0.19907,122.82,-0.11184,-0.29416,0.94919,256.93,-0.85254,-0.46235,-0.24374,154.51

> ui mousemode right "rotate selected models"

> view matrix models
> #2,-0.4007,-0.62248,-0.67228,111.87,-0.21408,-0.64984,0.7293,250.64,-0.89085,0.43616,0.12713,165.9

> ui mousemode right "translate selected models"

[Repeated 2 time(s)]

> view matrix models
> #2,-0.4007,-0.62248,-0.67228,113.93,-0.21408,-0.64984,0.7293,255.19,-0.89085,0.43616,0.12713,153.24

> ui tool show "Map Filter"

> volume gaussian #1 sDev 0.42

Opened cryosparc_P1_J482_004_volume_map.mrc gaussian as #3, grid size
352,352,352, pixel 0.846, shown at step 1, values float32  

> fitmap #2 inMap #1

Fit molecule AF-Q8TCS8-F1-model_v6.pdb (#2) to map
cryosparc_P1_J482_004_volume_map.mrc (#1) using 1256 atoms  
average map value = 0.04212, steps = 80  
shifted from previous position = 5.77  
rotated from previous position = 6.87 degrees  
atoms outside contour = 1022, contour level = 0.079169  
  
Position of AF-Q8TCS8-F1-model_v6.pdb (#2) relative to
cryosparc_P1_J482_004_volume_map.mrc (#1) coordinates:  
Matrix rotation and translation  
-0.14765691 -0.51103760 -0.84678093 101.20040909  
0.33912440 0.77810326 -0.52872480 135.10645661  
0.92908129 -0.36523397 0.05841302 88.77346607  
Axis 0.08275295 -0.89887510 0.43032024  
Axis point -0.71225091 0.00000000 127.79384097  
Rotation angle (degrees) 98.94987464  
Shift along axis -74.86817754  
  
Traceback (most recent call last):  
File
"/Applications/ChimeraX_Daily.app/Contents/Library/Frameworks/Python.framework/Versions/3.11/lib/python3.11/site-
packages/chimerax/segmentations/ui/orthoplanes.py", line 1925, in
_color_chosen  
self.plane_viewer.on_color_changed()  
File
"/Applications/ChimeraX_Daily.app/Contents/Library/Frameworks/Python.framework/Versions/3.11/lib/python3.11/site-
packages/chimerax/segmentations/ui/orthoplanes.py", line 754, in
on_color_changed  
colors = self.view.drawing.parent.image_colors  
^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^  
AttributeError: 'NoneType' object has no attribute 'image_colors'  
  
AttributeError: 'NoneType' object has no attribute 'image_colors'  
  
File
"/Applications/ChimeraX_Daily.app/Contents/Library/Frameworks/Python.framework/Versions/3.11/lib/python3.11/site-
packages/chimerax/segmentations/ui/orthoplanes.py", line 754, in
on_color_changed  
colors = self.view.drawing.parent.image_colors  
^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^  
  
See log for complete Python traceback.  
  

> view matrix models
> #2,-0.45927,-0.66663,-0.58709,119.96,-0.13295,-0.60189,0.78744,265.8,-0.87829,0.4397,0.1878,186.5

> hide #!3 models

> close #3

> view matrix models
> #2,0.77415,0.41998,-0.4736,92.737,-0.24702,-0.48843,-0.83691,197.65,-0.58281,0.76488,-0.27438,166.01

> view matrix models
> #2,0.75644,0.34979,0.55267,145.43,0.10707,0.76736,-0.63222,187.93,-0.64524,0.53741,0.54301,220.16

> view matrix models
> #2,0.70708,-0.70671,-0.024594,127.59,-0.32887,-0.35943,0.8733,284.08,-0.62601,-0.6094,-0.48656,169.56

> view matrix models
> #2,0.70708,-0.70671,-0.024594,129.66,-0.32887,-0.35943,0.8733,293.52,-0.62601,-0.6094,-0.48656,156.99

> view matrix models
> #2,0.99039,-0.12068,-0.067563,115.55,-0.028314,-0.65509,0.75502,280.22,-0.13537,-0.74586,-0.65221,150.64

> show #!1 models

> transparency 0

> transparency 50

> view matrix models
> #2,0.8449,0.48463,0.22644,131.66,0.11966,-0.58382,0.80302,264.44,0.52137,-0.65138,-0.55126,117.9

> view matrix models
> #2,0.38736,0.91086,0.1424,130.54,0.57477,-0.35937,0.73518,271.04,0.72083,-0.20294,-0.66274,108.58

> view matrix models
> #2,-0.38746,-0.87366,-0.29427,135.28,0.3028,-0.42209,0.85449,293.62,-0.87074,0.24197,0.42809,205.05

> ui mousemode right "rotate selected models"

> view matrix models
> #2,0.17337,-0.56863,-0.80411,96.434,-0.65603,-0.67565,0.33634,285.56,-0.73455,0.46921,-0.49018,153.04

> view matrix models
> #2,0.86201,-0.50664,0.016077,126.55,-0.38189,-0.62824,0.67785,298.11,-0.33332,-0.59045,-0.73503,143.5

> ui mousemode right "translate selected models"

> view matrix models
> #2,0.86201,-0.50664,0.016077,128.49,-0.38189,-0.62824,0.67785,285.93,-0.33332,-0.59045,-0.73503,131.16

> fitmap #2 inMap #1

Fit molecule AF-Q8TCS8-F1-model_v6.pdb (#2) to map
cryosparc_P1_J482_004_volume_map.mrc (#1) using 1256 atoms  
average map value = 0.06804, steps = 208  
shifted from previous position = 4.34  
rotated from previous position = 49.4 degrees  
atoms outside contour = 775, contour level = 0.079169  
  
Position of AF-Q8TCS8-F1-model_v6.pdb (#2) relative to
cryosparc_P1_J482_004_volume_map.mrc (#1) coordinates:  
Matrix rotation and translation  
0.04261260 0.99909171 -0.00032583 124.39404394  
0.54482783 -0.02351097 -0.83821837 104.44003070  
-0.83746464 0.03554113 -0.54533477 199.64463617  
Axis 0.67601140 0.64767865 -0.35145548  
Axis point 0.00000000 -69.26766377 146.15003188  
Rotation angle (degrees) 139.73972107  
Shift along axis 81.56916779  
  

> save
> /Users/madhurikanavalli/Desktop/2025/Cryosparc/Q387R/S1_KH_AF_Q8TCS8.pdb

> select #2/A:599-680

634 atoms, 645 bonds, 82 residues, 1 model selected  

> select #2/A:599-678

625 atoms, 636 bonds, 80 residues, 1 model selected  

> delete sel

> fitmap #2 inMap #1

Fit molecule AF-Q8TCS8-F1-model_v6.pdb (#2) to map
cryosparc_P1_J482_004_volume_map.mrc (#1) using 631 atoms  
average map value = 0.08786, steps = 116  
shifted from previous position = 6.49  
rotated from previous position = 8.34 degrees  
atoms outside contour = 299, contour level = 0.079169  
  
Position of AF-Q8TCS8-F1-model_v6.pdb (#2) relative to
cryosparc_P1_J482_004_volume_map.mrc (#1) coordinates:  
Matrix rotation and translation  
0.08642435 0.98758308 -0.13118947 114.63946268  
0.49800827 -0.15687479 -0.85286471 106.34543108  
-0.86285503 0.00837483 -0.50538229 203.22585816  
Axis 0.69930517 0.59409431 -0.39752262  
Axis point 0.00000000 -51.69253347 151.23207638  
Rotation angle (degrees) 141.99121366  
Shift along axis 62.56030846  
  

> select add #2

631 atoms, 640 bonds, 81 residues, 1 model selected  

> view matrix models
> #2,-0.12421,-0.93788,0.32398,174.03,-0.66983,0.32014,0.66996,284.8,-0.73205,-0.13379,-0.66798,133.24

> fitmap #2 inMap #1

Fit molecule AF-Q8TCS8-F1-model_v6.pdb (#2) to map
cryosparc_P1_J482_004_volume_map.mrc (#1) using 631 atoms  
average map value = 0.06691, steps = 176  
shifted from previous position = 13.3  
rotated from previous position = 44.4 degrees  
atoms outside contour = 409, contour level = 0.079169  
  
Position of AF-Q8TCS8-F1-model_v6.pdb (#2) relative to
cryosparc_P1_J482_004_volume_map.mrc (#1) coordinates:  
Matrix rotation and translation  
0.08570214 0.84446583 0.52870857 166.53390201  
0.80947457 0.25036444 -0.53110145 116.83892504  
-0.58086681 0.47349266 -0.66211680 176.42251434  
Axis 0.67098439 0.74110306 -0.02337122  
Axis point 85.33225300 0.00000000 79.05264705  
Rotation angle (degrees) 131.53099228  
Shift along axis 194.20812316  
  

> undo

> ui tool show "Fit Loops"

> select #2/A:678-750

568 atoms, 576 bonds, 72 residues, 1 model selected  

> help help:user/selection.html

> select #2/A:678-759

631 atoms, 640 bonds, 81 residues, 1 model selected  

> select add #1

631 atoms, 640 bonds, 81 residues, 3 models selected  

> ui tool show "Fit Loops"

> select #2/A:678-759

631 atoms, 640 bonds, 81 residues, 1 model selected  

> view matrix models
> #2,-0.080102,0.99257,0.091628,132.2,0.78592,0.11943,-0.60668,151.24,-0.61311,0.023417,-0.78965,108.42

> view matrix models
> #2,-0.72601,0.14287,0.67268,201.64,0.41779,0.8686,0.26644,223.05,-0.54622,0.47448,-0.6903,110.38

> view matrix models
> #2,0.41003,-0.81571,-0.40804,121.18,-0.77968,-0.54561,0.30724,268.36,-0.47325,0.19216,-0.85971,105.09

> fitmap #2 inMap #1

Fit molecule AF-Q8TCS8-F1-model_v6.pdb (#2) to map
cryosparc_P1_J482_004_volume_map.mrc (#1) using 631 atoms  
average map value = 0.06322, steps = 88  
shifted from previous position = 3.4  
rotated from previous position = 9.25 degrees  
atoms outside contour = 424, contour level = 0.079169  
  
Position of AF-Q8TCS8-F1-model_v6.pdb (#2) relative to
cryosparc_P1_J482_004_volume_map.mrc (#1) coordinates:  
Matrix rotation and translation  
0.03035950 0.89245803 0.45010791 155.53133934  
0.87362537 0.19510483 -0.44577237 120.58172191  
-0.48565132 0.40675911 -0.77375062 165.41563616  
Axis 0.67339426 0.73913388 -0.01487547  
Axis point 63.44943971 0.00000000 77.49031568  
Rotation angle (degrees) 140.72740844  
Shift along axis 191.39931233  
  

> view matrix models
> #2,-0.2609,0.80697,0.52985,161.6,-0.79532,-0.49076,0.35582,304.63,0.54717,-0.32857,0.76984,238.37

> view matrix models
> #2,-0.2609,0.80697,0.52985,162.1,-0.79532,-0.49076,0.35582,311.26,0.54717,-0.32857,0.76984,235.39

> ui mousemode right "rotate selected models"

> view matrix models
> #2,0.79732,-0.18475,-0.57458,82.674,0.32329,0.93464,0.14809,246.3,0.50967,-0.30383,0.80494,238.13

> view matrix models
> #2,-0.26525,0.76742,0.5837,166.72,0.96297,0.1805,0.2003,251.51,0.048357,0.61522,-0.78687,118.67

> view matrix models
> #2,-0.5306,0.57497,0.62279,178.59,0.83975,0.45646,0.29404,255.22,-0.11521,0.67901,-0.72503,125.08

> ui mousemode right "translate selected models"

> view matrix models
> #2,-0.5306,0.57497,0.62279,182.25,0.83975,0.45646,0.29404,229.39,-0.11521,0.67901,-0.72503,101.71

> fitmap #2 inMap #1

Fit molecule AF-Q8TCS8-F1-model_v6.pdb (#2) to map
cryosparc_P1_J482_004_volume_map.mrc (#1) using 631 atoms  
average map value = 0.07811, steps = 376  
shifted from previous position = 14.4  
rotated from previous position = 20.2 degrees  
atoms outside contour = 373, contour level = 0.079169  
  
Position of AF-Q8TCS8-F1-model_v6.pdb (#2) relative to
cryosparc_P1_J482_004_volume_map.mrc (#1) coordinates:  
Matrix rotation and translation  
-0.07173609 0.89418681 0.44190955 152.95290020  
0.99703897 0.07659145 0.00687161 107.65452962  
-0.02770198 0.44109399 -0.89703339 138.81763398  
Axis 0.67028894 0.72491753 0.15876812  
Axis point 38.94893660 -0.00000000 55.65097782  
Rotation angle (degrees) 161.10063978  
Shift along axis 202.60310684  
  

> fitmap #2 inMap #1

Fit molecule AF-Q8TCS8-F1-model_v6.pdb (#2) to map
cryosparc_P1_J482_004_volume_map.mrc (#1) using 631 atoms  
average map value = 0.07811, steps = 60  
shifted from previous position = 0.00204  
rotated from previous position = 0.0118 degrees  
atoms outside contour = 372, contour level = 0.079169  
  
Position of AF-Q8TCS8-F1-model_v6.pdb (#2) relative to
cryosparc_P1_J482_004_volume_map.mrc (#1) coordinates:  
Matrix rotation and translation  
-0.07176106 0.89413760 0.44200504 152.95957123  
0.99704175 0.07653997 0.00703955 107.66663533  
-0.02753672 0.44120265 -0.89698504 138.81658330  
Axis 0.67028233 0.72490165 0.15886848  
Axis point 38.94180658 0.00000000 55.64258504  
Rotation angle (degrees) 161.10312462  
Shift along axis 202.62739965  
  

> fitmap #2 inMap #1 search 100

Found 87 unique fits from 100 random placements having fraction of points
inside contour >= 0.100 (87 of 100).  
  
Average map values and times found:  
0.08675 (1), 0.0858 (1), 0.08496 (1), 0.08402 (1), 0.08091 (1), 0.07993 (1),
0.07989 (1), 0.07882 (1), 0.07649 (1), 0.07503 (1), 0.07278 (1), 0.07178 (1),
0.07137 (1), 0.07135 (1), 0.07125 (1), 0.07038 (1), 0.06968 (1), 0.0696 (1),
0.06958 (1), 0.06919 (1), 0.06882 (1), 0.06881 (1), 0.06872 (1), 0.06856 (1),
0.06817 (1), 0.06804 (1), 0.06775 (1), 0.067 (1), 0.06694 (1), 0.06685 (1),
0.06679 (1), 0.06644 (1), 0.06608 (1), 0.06546 (1), 0.06522 (1), 0.06505 (1),
0.06501 (1), 0.06456 (1), 0.06449 (1), 0.06445 (1), 0.06412 (1), 0.06391 (1),
0.06383 (1), 0.06328 (1), 0.06317 (1), 0.06302 (1), 0.06279 (1), 0.06259 (1),
0.06247 (1), 0.06224 (1), 0.06208 (1), 0.06175 (1), 0.06135 (1), 0.06096 (1),
0.06089 (1), 0.06063 (1), 0.06061 (1), 0.06049 (1), 0.06034 (1), 0.06004 (1),
0.05993 (1), 0.05944 (1), 0.05883 (1), 0.05862 (1), 0.05856 (1), 0.05812 (1),
0.05806 (1), 0.05762 (1), 0.05748 (1), 0.05743 (1), 0.05698 (1), 0.05681 (1),
0.05641 (1), 0.05503 (1), 0.05483 (1), 0.05464 (1), 0.05438 (1), 0.05405 (1),
0.05346 (1), 0.05336 (1), 0.05331 (1), 0.05263 (1), 0.05247 (1), 0.05073 (1),
0.04919 (1), 0.04544 (1), 0.0362 (1)  
  
Best fit found:  
Fit molecule AF-Q8TCS8-F1-model_v6.pdb (#2) to map
cryosparc_P1_J482_004_volume_map.mrc (#1) using 631 atoms  
average map value = 0.08675, steps = 68  
shifted from previous position = 1.68  
rotated from previous position = 1.81 degrees  
atoms outside contour = 358, contour level = 0.079169  
Position of AF-Q8TCS8-F1-model_v6.pdb (#2) relative to
cryosparc_P1_J482_004_volume_map.mrc (#1) coordinates:  
Matrix rotation and translation  
0.70736822 -0.65675968 0.26133708 180.10612296  
0.21398252 -0.15340721 -0.96471646 104.42350305  
0.67367783 0.73833129 0.03201965 146.82400859  
Axis 0.87037715 -0.21073512 0.44501049  
Axis point 0.00000000 47.11093603 95.62601638  
Rotation angle (degrees) 101.94716792  
Shift along axis 200.09277743  
  
Found 87 fits.  

> hide #3 models

> hide #4 models

> hide #5 models

> hide #6 models

> hide #7 models

> hide #8 models

> hide #9 models

> hide #10 models

> hide #11 models

> hide #12 models

> show #10 models

> hide #2 models

> select subtract #2

Nothing selected  

> select add #10

631 atoms, 640 bonds, 81 residues, 1 model selected  

> view matrix models
> #10,-0.76824,-0.34676,-0.53812,99.406,-0.30813,-0.53651,0.78563,251.71,-0.56113,0.76936,0.30532,130.5

> view matrix models
> #10,0.0083816,-0.32273,-0.94645,48.86,-0.68359,-0.69264,0.23013,270.4,-0.72982,0.64506,-0.22642,136.05

> view matrix models
> #10,0.40111,0.42379,-0.8121,36.805,-0.50188,-0.63997,-0.58186,252.21,-0.76631,0.64097,-0.044006,144.17

> view matrix models
> #10,0.42445,0.50945,-0.74854,38.601,0.14388,-0.85414,-0.49974,187.19,-0.89395,0.10441,-0.43584,131.32

> view matrix models
> #10,0.14263,0.44845,0.88235,158.31,0.50713,0.73245,-0.45424,158.91,-0.84999,0.51225,-0.12295,150.79

> view matrix models
> #10,-0.53518,0.37484,0.75702,165.86,0.57045,0.82133,-0.0034073,164.81,-0.62304,0.43002,-0.65338,103.74

> view matrix models
> #10,-0.3295,0.66242,0.67278,141.81,0.70653,0.64567,-0.2897,186.36,-0.6263,0.37989,-0.68076,156.02

Desktop color scheme is dark  

> close session

> open /Users/madhurikanavalli/Downloads/cryosparc_P1_J275_004_volume_map.mrc

Opened cryosparc_P1_J275_004_volume_map.mrc as #1, grid size 450,450,450,
pixel 0.846, shown at level 0.00835, step 2, values float32  

> volume #1 level 0.03433

> open /Users/madhurikanavalli/Downloads/cryosparc_P1_J482_004_volume_map.mrc

Opened cryosparc_P1_J482_004_volume_map.mrc as #2, grid size 352,352,352,
pixel 0.846, shown at level 0.0477, step 2, values float32  

> tile

2 models tiled  
Traceback (most recent call last):  
File
"/Applications/ChimeraX_Daily.app/Contents/Library/Frameworks/Python.framework/Versions/3.11/lib/python3.11/site-
packages/chimerax/map/volume_viewer.py", line 2178, in mouseMoveEvent  
self._drag(event)  
File
"/Applications/ChimeraX_Daily.app/Contents/Library/Frameworks/Python.framework/Versions/3.11/lib/python3.11/site-
packages/chimerax/map/volume_viewer.py", line 2182, in _drag  
cb(event)  
File
"/Applications/ChimeraX_Daily.app/Contents/Library/Frameworks/Python.framework/Versions/3.11/lib/python3.11/site-
packages/chimerax/map/histogram.py", line 416, in move_marker_cb  
cb(m)  
File
"/Applications/ChimeraX_Daily.app/Contents/Library/Frameworks/Python.framework/Versions/3.11/lib/python3.11/site-
packages/chimerax/segmentations/ui/orthoplanes.py", line 1931, in
moved_marker_cb  
self.plane_viewer.on_color_changed()  
File
"/Applications/ChimeraX_Daily.app/Contents/Library/Frameworks/Python.framework/Versions/3.11/lib/python3.11/site-
packages/chimerax/segmentations/ui/orthoplanes.py", line 754, in
on_color_changed  
colors = self.view.drawing.parent.image_colors  
^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^  
AttributeError: 'NoneType' object has no attribute 'image_colors'  
  
AttributeError: 'NoneType' object has no attribute 'image_colors'  
  
File
"/Applications/ChimeraX_Daily.app/Contents/Library/Frameworks/Python.framework/Versions/3.11/lib/python3.11/site-
packages/chimerax/segmentations/ui/orthoplanes.py", line 754, in
on_color_changed  
colors = self.view.drawing.parent.image_colors  
^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^  
  
See log for complete Python traceback.  
  
Traceback (most recent call last):  
File
"/Applications/ChimeraX_Daily.app/Contents/Library/Frameworks/Python.framework/Versions/3.11/lib/python3.11/site-
packages/chimerax/map/volume_viewer.py", line 2178, in mouseMoveEvent  
self._drag(event)  
File
"/Applications/ChimeraX_Daily.app/Contents/Library/Frameworks/Python.framework/Versions/3.11/lib/python3.11/site-
packages/chimerax/map/volume_viewer.py", line 2182, in _drag  
cb(event)  
File
"/Applications/ChimeraX_Daily.app/Contents/Library/Frameworks/Python.framework/Versions/3.11/lib/python3.11/site-
packages/chimerax/map/histogram.py", line 416, in move_marker_cb  
cb(m)  
File
"/Applications/ChimeraX_Daily.app/Contents/Library/Frameworks/Python.framework/Versions/3.11/lib/python3.11/site-
packages/chimerax/segmentations/ui/orthoplanes.py", line 1931, in
moved_marker_cb  
self.plane_viewer.on_color_changed()  
File
"/Applications/ChimeraX_Daily.app/Contents/Library/Frameworks/Python.framework/Versions/3.11/lib/python3.11/site-
packages/chimerax/segmentations/ui/orthoplanes.py", line 754, in
on_color_changed  
colors = self.view.drawing.parent.image_colors  
^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^  
AttributeError: 'NoneType' object has no attribute 'image_colors'  
  
AttributeError: 'NoneType' object has no attribute 'image_colors'  
  
File
"/Applications/ChimeraX_Daily.app/Contents/Library/Frameworks/Python.framework/Versions/3.11/lib/python3.11/site-
packages/chimerax/segmentations/ui/orthoplanes.py", line 754, in
on_color_changed  
colors = self.view.drawing.parent.image_colors  
^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^  
  
See log for complete Python traceback.  
  

> color #2 #7a81ffff models

Traceback (most recent call last):  
File
"/Applications/ChimeraX_Daily.app/Contents/Library/Frameworks/Python.framework/Versions/3.11/lib/python3.11/site-
packages/chimerax/map/volume_viewer.py", line 2178, in mouseMoveEvent  
self._drag(event)  
File
"/Applications/ChimeraX_Daily.app/Contents/Library/Frameworks/Python.framework/Versions/3.11/lib/python3.11/site-
packages/chimerax/map/volume_viewer.py", line 2182, in _drag  
cb(event)  
File
"/Applications/ChimeraX_Daily.app/Contents/Library/Frameworks/Python.framework/Versions/3.11/lib/python3.11/site-
packages/chimerax/map/histogram.py", line 416, in move_marker_cb  
cb(m)  
File
"/Applications/ChimeraX_Daily.app/Contents/Library/Frameworks/Python.framework/Versions/3.11/lib/python3.11/site-
packages/chimerax/segmentations/ui/orthoplanes.py", line 1931, in
moved_marker_cb  
self.plane_viewer.on_color_changed()  
File
"/Applications/ChimeraX_Daily.app/Contents/Library/Frameworks/Python.framework/Versions/3.11/lib/python3.11/site-
packages/chimerax/segmentations/ui/orthoplanes.py", line 754, in
on_color_changed  
colors = self.view.drawing.parent.image_colors  
^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^  
AttributeError: 'NoneType' object has no attribute 'image_colors'  
  
AttributeError: 'NoneType' object has no attribute 'image_colors'  
  
File
"/Applications/ChimeraX_Daily.app/Contents/Library/Frameworks/Python.framework/Versions/3.11/lib/python3.11/site-
packages/chimerax/segmentations/ui/orthoplanes.py", line 754, in
on_color_changed  
colors = self.view.drawing.parent.image_colors  
^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^  
  
See log for complete Python traceback.  
  

> tile

2 models tiled  
Traceback (most recent call last):  
File
"/Applications/ChimeraX_Daily.app/Contents/Library/Frameworks/Python.framework/Versions/3.11/lib/python3.11/site-
packages/chimerax/map/volume_viewer.py", line 2178, in mouseMoveEvent  
self._drag(event)  
File
"/Applications/ChimeraX_Daily.app/Contents/Library/Frameworks/Python.framework/Versions/3.11/lib/python3.11/site-
packages/chimerax/map/volume_viewer.py", line 2182, in _drag  
cb(event)  
File
"/Applications/ChimeraX_Daily.app/Contents/Library/Frameworks/Python.framework/Versions/3.11/lib/python3.11/site-
packages/chimerax/map/histogram.py", line 416, in move_marker_cb  
cb(m)  
File
"/Applications/ChimeraX_Daily.app/Contents/Library/Frameworks/Python.framework/Versions/3.11/lib/python3.11/site-
packages/chimerax/segmentations/ui/orthoplanes.py", line 1931, in
moved_marker_cb  
self.plane_viewer.on_color_changed()  
File
"/Applications/ChimeraX_Daily.app/Contents/Library/Frameworks/Python.framework/Versions/3.11/lib/python3.11/site-
packages/chimerax/segmentations/ui/orthoplanes.py", line 754, in
on_color_changed  
colors = self.view.drawing.parent.image_colors  
^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^  
AttributeError: 'NoneType' object has no attribute 'image_colors'  
  
AttributeError: 'NoneType' object has no attribute 'image_colors'  
  
File
"/Applications/ChimeraX_Daily.app/Contents/Library/Frameworks/Python.framework/Versions/3.11/lib/python3.11/site-
packages/chimerax/segmentations/ui/orthoplanes.py", line 754, in
on_color_changed  
colors = self.view.drawing.parent.image_colors  
^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^  
  
See log for complete Python traceback.  
  
Traceback (most recent call last):  
File
"/Applications/ChimeraX_Daily.app/Contents/Library/Frameworks/Python.framework/Versions/3.11/lib/python3.11/site-
packages/chimerax/segmentations/ui/orthoplanes.py", line 1777, in <lambda>  
sm.addAction("%d" % step, lambda s=step: self.data_step_cb(s))  
^^^^^^^^^^^^^^^^^^^^  
File
"/Applications/ChimeraX_Daily.app/Contents/Library/Frameworks/Python.framework/Versions/3.11/lib/python3.11/site-
packages/chimerax/segmentations/ui/orthoplanes.py", line 1850, in data_step_cb  
self.plane_viewer.update_and_rerender()  
File
"/Applications/ChimeraX_Daily.app/Contents/Library/Frameworks/Python.framework/Versions/3.11/lib/python3.11/site-
packages/chimerax/segmentations/ui/orthoplanes.py", line 833, in
update_and_rerender  
self.view.drawing.parent.update_drawings()  
^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^  
AttributeError: 'NoneType' object has no attribute 'update_drawings'  
  
AttributeError: 'NoneType' object has no attribute 'update_drawings'  
  
File
"/Applications/ChimeraX_Daily.app/Contents/Library/Frameworks/Python.framework/Versions/3.11/lib/python3.11/site-
packages/chimerax/segmentations/ui/orthoplanes.py", line 833, in
update_and_rerender  
self.view.drawing.parent.update_drawings()  
^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^  
  
See log for complete Python traceback.  
  




OpenGL version: 4.1 Metal - 90.5
OpenGL renderer: Apple M1
OpenGL vendor: Apple

Python: 3.11.9
Locale: en_US.UTF-8
Qt version: PyQt6 6.9.1, Qt 6.9.0
Qt runtime version: 6.9.1
Qt platform: cocoa
Hardware:

    Hardware Overview:

      Model Name: MacBook Pro
      Model Identifier: MacBookPro17,1
      Model Number: Z11B0002QZE/A
      Chip: Apple M1
      Total Number of Cores: 8 (4 performance and 4 efficiency)
      Memory: 16 GB
      System Firmware Version: 13822.1.2
      OS Loader Version: 13822.1.2

Software:

    System Software Overview:

      System Version: macOS 26.0.1 (25A362)
      Kernel Version: Darwin 25.0.0
      Time since boot: 2 days, 4 hours, 34 minutes

Graphics/Displays:

    Apple M1:

      Chipset Model: Apple M1
      Type: GPU
      Bus: Built-In
      Total Number of Cores: 8
      Vendor: Apple (0x106b)
      Metal Support: Metal 4
      Displays:
        Color LCD:
          Display Type: Built-In Retina LCD
          Resolution: 2560 x 1600 Retina
          Main Display: Yes
          Mirror: Off
          Online: Yes
          Automatically Adjust Brightness: Yes
          Connection Type: Internal


Installed Packages:
    alabaster: 1.0.0
    appdirs: 1.4.4
    appnope: 0.1.4
    asttokens: 3.0.0
    babel: 2.17.0
    beautifulsoup4: 4.13.5
    blockdiag: 3.0.0
    blosc2: 3.7.2
    build: 1.3.0
    certifi: 2025.7.14
    cftime: 1.6.4.post1
    charset-normalizer: 3.4.3
    ChimeraX-AddCharge: 1.5.20
    ChimeraX-AddH: 2.2.7
    ChimeraX-AlignmentAlgorithms: 2.0.2
    ChimeraX-AlignmentHdrs: 3.6.1
    ChimeraX-AlignmentMatrices: 2.1
    ChimeraX-Alignments: 3.0.2
    ChimeraX-AlphaFold: 1.0.1
    ChimeraX-AltlocExplorer: 1.1.2
    ChimeraX-AmberInfo: 1.0
    ChimeraX-Aniso: 1.3.2
    ChimeraX-Arrays: 1.1
    ChimeraX-Atomic: 1.60.16
    ChimeraX-AtomicLibrary: 14.1.23
    ChimeraX-AtomSearch: 2.0.1
    ChimeraX-AxesPlanes: 2.4
    ChimeraX-BasicActions: 1.1.3
    ChimeraX-BILD: 1.0
    ChimeraX-BlastProtein: 3.0.0
    ChimeraX-Boltz: 1.1
    ChimeraX-BondRot: 2.0.4
    ChimeraX-BugReporter: 1.0.2
    ChimeraX-BuildStructure: 2.13.1
    ChimeraX-Bumps: 1.0
    ChimeraX-BundleBuilder: 1.6.0
    ChimeraX-ButtonPanel: 1.0.1
    ChimeraX-CageBuilder: 1.0.1
    ChimeraX-CellPack: 1.0
    ChimeraX-Centroids: 1.4
    ChimeraX-ChangeChains: 1.1
    ChimeraX-CheckWaters: 1.5
    ChimeraX-ChemGroup: 2.0.2
    ChimeraX-Clashes: 2.3
    ChimeraX-clix: 0.2.4
    ChimeraX-ColorActions: 1.0.5
    ChimeraX-ColorGlobe: 1.0
    ChimeraX-ColorKey: 1.5.8
    ChimeraX-CommandLine: 1.3.0
    ChimeraX-ConnectStructure: 2.0.1
    ChimeraX-Contacts: 1.0.1
    ChimeraX-Core: 1.11.dev202509040038
    ChimeraX-CoreFormats: 1.2
    ChimeraX-coulombic: 1.4.5
    ChimeraX-Crosslinks: 1.0
    ChimeraX-Crystal: 1.0
    ChimeraX-CrystalContacts: 1.0.1
    ChimeraX-DataFormats: 1.2.4
    ChimeraX-Dicom: 1.2.7
    ChimeraX-DistMonitor: 1.4.2
    ChimeraX-DockPrep: 1.1.4
    ChimeraX-Dssp: 2.0
    ChimeraX-EMalign: 0.1.3
    ChimeraX-EMDB-SFF: 1.0
    ChimeraX-ESMFold: 1.0
    ChimeraX-FileHistory: 1.0.1
    ChimeraX-FunctionKey: 1.0.1
    ChimeraX-Geometry: 1.3
    ChimeraX-gltf: 1.0
    ChimeraX-Graphics: 1.4.1
    ChimeraX-Hbonds: 2.5.3
    ChimeraX-Help: 1.3
    ChimeraX-HKCage: 1.3
    ChimeraX-IHM: 1.1
    ChimeraX-ImageFormats: 1.2
    ChimeraX-IMOD: 1.0
    ChimeraX-IO: 1.0.4
    ChimeraX-ItemsInspection: 1.0.1
    ChimeraX-IUPAC: 1.0
    ChimeraX-KVFinder: 1.7.1
    ChimeraX-Label: 1.2
    ChimeraX-ListInfo: 1.2.2
    ChimeraX-Log: 1.2
    ChimeraX-LookingGlass: 1.1
    ChimeraX-Maestro: 1.9.2
    ChimeraX-Map: 1.3
    ChimeraX-MapData: 2.0
    ChimeraX-MapEraser: 1.0.1
    ChimeraX-MapFilter: 2.0.1
    ChimeraX-MapFit: 2.0
    ChimeraX-MapSeries: 2.1.1
    ChimeraX-Markers: 1.0.1
    ChimeraX-Mask: 1.0.2
    ChimeraX-maskChains: 1.4
    ChimeraX-MatchMaker: 2.2.2
    ChimeraX-MCopy: 1.0
    ChimeraX-MDcrds: 2.17.1
    ChimeraX-MedicalToolbar: 1.1
    ChimeraX-Meeting: 1.0.1
    ChimeraX-Minimize: 1.2
    ChimeraX-MLP: 1.1.1
    ChimeraX-mmCIF: 2.16
    ChimeraX-MMTF: 2.2
    ChimeraX-ModelArchive: 1.0
    ChimeraX-Modeller: 1.5.22
    ChimeraX-ModelPanel: 1.5.1
    ChimeraX-ModelSeries: 1.0.1
    ChimeraX-Mol2: 2.0.3
    ChimeraX-Mole: 1.0
    ChimeraX-Morph: 1.0.2
    ChimeraX-MouseModes: 1.2
    ChimeraX-Movie: 1.0.1
    ChimeraX-MutationScores: 1.0
    ChimeraX-Neuron: 1.0
    ChimeraX-Nifti: 1.2
    ChimeraX-NMRSTAR: 1.0.2
    ChimeraX-NRRD: 1.2
    ChimeraX-Nucleotides: 2.0.3
    ChimeraX-OpenCommand: 1.15.1
    ChimeraX-OrthoPick: 1.0.1
    ChimeraX-PDB: 2.7.10
    ChimeraX-PDBBio: 1.0.1
    ChimeraX-PDBLibrary: 1.0.4
    ChimeraX-PDBMatrices: 1.0
    ChimeraX-PhenixUI: 1.3.9
    ChimeraX-PickBlobs: 1.0.1
    ChimeraX-Positions: 1.0
    ChimeraX-PresetMgr: 1.1.3
    ChimeraX-ProfileGrids: 1.3.1
    ChimeraX-PubChem: 2.2
    ChimeraX-QScore: 1.2
    ChimeraX-ReadPbonds: 1.0.1
    ChimeraX-Registration: 1.1.2
    ChimeraX-RemoteControl: 1.0
    ChimeraX-RenderByAttr: 1.6.5
    ChimeraX-RenumberResidues: 1.1
    ChimeraX-ResidueFit: 1.0.1
    ChimeraX-RestServer: 1.3.1
    ChimeraX-RNALayout: 1.0
    ChimeraX-RotamerLibMgr: 4.0
    ChimeraX-RotamerLibsDunbrack: 2.0
    ChimeraX-RotamerLibsDynameomics: 2.0
    ChimeraX-RotamerLibsRichardson: 2.0
    ChimeraX-SaveCommand: 1.5.2
    ChimeraX-Scenes: 0.2.1
    ChimeraX-SchemeMgr: 1.0
    ChimeraX-SDF: 2.0.3
    ChimeraX-Segger: 1.0
    ChimeraX-Segment: 1.0.1
    ChimeraX-Segmentations: 3.5.7
    ChimeraX-SelInspector: 1.0
    ChimeraX-SeqView: 2.17.2
    ChimeraX-Shape: 1.1
    ChimeraX-Shell: 1.0.1
    ChimeraX-Shortcuts: 1.2.1
    ChimeraX-ShowSequences: 1.0.3
    ChimeraX-SideView: 1.0.1
    ChimeraX-SimilarStructures: 1.0.1
    ChimeraX-Smiles: 2.1.2
    ChimeraX-SmoothLines: 1.0
    ChimeraX-SpaceNavigator: 1.0
    ChimeraX-StdCommands: 1.19.1
    ChimeraX-STL: 1.0.1
    ChimeraX-Storm: 1.0
    ChimeraX-StructMeasure: 1.2.1
    ChimeraX-Struts: 1.0.1
    ChimeraX-Surface: 1.0.1
    ChimeraX-SwapAA: 2.0.1
    ChimeraX-SwapRes: 2.5.2
    ChimeraX-TapeMeasure: 1.0
    ChimeraX-TaskManager: 1.0
    ChimeraX-Test: 1.0
    ChimeraX-Toolbar: 1.2.3
    ChimeraX-ToolshedUtils: 1.2.4
    ChimeraX-Topography: 1.0
    ChimeraX-ToQuest: 1.0
    ChimeraX-Tug: 1.0.1
    ChimeraX-UI: 1.48.2
    ChimeraX-Umap: 1.0
    ChimeraX-uniprot: 2.3.1
    ChimeraX-UnitCell: 1.0.1
    ChimeraX-ViewDock: 1.3.2
    ChimeraX-VIPERdb: 1.0
    ChimeraX-Vive: 1.1
    ChimeraX-VolumeMenu: 1.0.1
    ChimeraX-vrml: 1.0
    ChimeraX-VTK: 1.0
    ChimeraX-WavefrontOBJ: 1.0
    ChimeraX-WebCam: 1.0.2
    ChimeraX-WebServices: 1.1.5
    ChimeraX-Zone: 1.0.1
    colorama: 0.4.6
    comm: 0.2.3
    contourpy: 1.3.3
    coverage: 7.10.6
    cxservices: 1.2.3
    cycler: 0.12.1
    Cython: 3.1.3
    debugpy: 1.8.16
    decorator: 5.2.1
    docutils: 0.21.2
    executing: 2.2.1
    filelock: 3.19.1
    finufft: 2.4.1
    fonttools: 4.59.2
    funcparserlib: 2.0.0a0
    glfw: 2.9.0
    grako: 3.16.5
    h5py: 3.14.0
    html2text: 2025.4.15
    idna: 3.10
    ihm: 2.2
    imagecodecs: 2024.6.1
    imagesize: 1.4.1
    iniconfig: 2.1.0
    ipykernel: 6.30.1
    ipython: 9.5.0
    ipython_pygments_lexers: 1.1.1
    ipywidgets: 8.1.7
    jedi: 0.19.2
    Jinja2: 3.1.6
    jupyter_client: 8.6.3
    jupyter_core: 5.8.1
    jupyterlab_widgets: 3.0.15
    kiwisolver: 1.4.9
    line_profiler: 5.0.0
    lxml: 6.0.1
    lz4: 4.3.2
    Markdown: 3.8.2
    MarkupSafe: 3.0.2
    matplotlib: 3.10.5
    matplotlib-inline: 0.1.7
    msgpack: 1.1.1
    ndindex: 1.10.0
    nest-asyncio: 1.6.0
    netCDF4: 1.6.5
    networkx: 3.3
    nibabel: 5.2.0
    nptyping: 2.5.0
    numexpr: 2.11.0
    numpy: 1.26.4
    OpenMM: 8.2.0
    openvr: 1.26.701
    packaging: 25.0
    ParmEd: 4.2.2
    parso: 0.8.5
    pep517: 0.13.1
    pexpect: 4.9.0
    pickleshare: 0.7.5
    pillow: 11.3.0
    pip: 25.2
    pkginfo: 1.12.1.2
    platformdirs: 4.4.0
    pluggy: 1.6.0
    prompt_toolkit: 3.0.52
    psutil: 7.0.0
    ptyprocess: 0.7.0
    pure_eval: 0.2.3
    py-cpuinfo: 9.0.0
    pybind11: 3.0.1
    pycollada: 0.8
    pydicom: 2.4.4
    Pygments: 2.18.0
    pynmrstar: 3.3.5
    pynrrd: 1.0.0
    PyOpenGL: 3.1.10
    PyOpenGL-accelerate: 3.1.10
    pyopenxr: 1.1.4501
    pyparsing: 3.2.3
    pyproject_hooks: 1.2.0
    PyQt6-commercial: 6.9.1
    PyQt6-Qt6: 6.9.1
    PyQt6-WebEngine-commercial: 6.9.0
    PyQt6-WebEngine-Qt6: 6.9.1
    PyQt6_sip: 13.10.2
    pytest: 8.4.1
    pytest-cov: 6.2.1
    python-dateutil: 2.9.0.post0
    pytz: 2025.2
    pyzmq: 27.0.2
    qtconsole: 5.6.1
    QtPy: 2.4.3
    qtshim: 1.2
    RandomWords: 0.4.0
    requests: 2.32.4
    roman-numerals-py: 3.1.0
    scipy: 1.14.0
    setuptools: 80.9.0
    sfftk-rw: 0.8.1
    six: 1.17.0
    snowballstemmer: 3.0.1
    sortedcontainers: 2.4.0
    soupsieve: 2.8
    Sphinx: 8.2.3
    sphinx-autodoc-typehints: 3.2.0
    sphinxcontrib-applehelp: 2.0.0
    sphinxcontrib-blockdiag: 3.0.0
    sphinxcontrib-devhelp: 2.0.0
    sphinxcontrib-htmlhelp: 2.1.0
    sphinxcontrib-jsmath: 1.0.1
    sphinxcontrib-qthelp: 2.0.0
    sphinxcontrib-serializinghtml: 2.0.0
    stack-data: 0.6.3
    superqt: 0.7.6
    tables: 3.10.2
    tcia_utils: 1.5.1
    tifffile: 2025.3.13
    tinyarray: 1.2.5
    tornado: 6.5.2
    traitlets: 5.14.3
    typing_extensions: 4.15.0
    tzdata: 2025.2
    urllib3: 2.5.0
    wcwidth: 0.2.13
    webcolors: 24.11.1
    wheel: 0.45.1
    wheel-filename: 1.4.2
    widgetsnbextension: 4.0.14

Change History (2)

comment:1 by Eric Pettersen, 4 weeks ago

Component: UnassignedDICOM
Description: modified (diff)
Owner: set to Zach Pearson
Platform: all
Project: ChimeraX
Status: newassigned
Summary: ChimeraX bug report submissionOrthoplanes: 'NoneType' object has no attribute 'image_colors'

comment:2 by Eric Pettersen, 4 weeks ago

Resolution: duplicate
Status: assignedclosed
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