Opened 7 weeks ago

Closed 7 weeks ago

#18672 closed defect (nonchimerax)

Crash in glBufferSubData

Reported by: chimerax-bug-report@… Owned by: Tom Goddard
Priority: normal Milestone:
Component: Graphics Version:
Keywords: Cc:
Blocked By: Blocking:
Notify when closed: Platform: all
Project: ChimeraX

Description (last modified by pett)

The following bug report has been submitted:
Platform:        macOS-14.5-arm64-arm-64bit
ChimeraX Version: 1.6.1 (2023-05-09 17:57:07 UTC)
Description
Last time you used ChimeraX it crashed.
Please describe steps that led to the crash here.
Fatal Python error: Segmentation fault

Current thread 0x0000000204ae8c00 (most recent call first):
  File "/Applications/ChimeraX-1.6.1.app/Contents/Library/Frameworks/Python.framework/Versions/3.9/lib/python3.9/site-packages/OpenGL/GL/VERSION/GL_1_5.py", line 125 in glBufferSubData
  File "/Applications/ChimeraX-1.6.1.app/Contents/Library/Frameworks/Python.framework/Versions/3.9/lib/python3.9/site-packages/chimerax/graphics/opengl.py", line 2702 in update_buffer_data
  File "/Applications/ChimeraX-1.6.1.app/Contents/Library/Frameworks/Python.framework/Versions/3.9/lib/python3.9/site-packages/chimerax/graphics/drawing.py", line 1903 in update_buffers
  File "/Applications/ChimeraX-1.6.1.app/Contents/Library/Frameworks/Python.framework/Versions/3.9/lib/python3.9/site-packages/chimerax/graphics/drawing.py", line 1915 in activate_bindings
  File "/Applications/ChimeraX-1.6.1.app/Contents/Library/Frameworks/Python.framework/Versions/3.9/lib/python3.9/site-packages/chimerax/graphics/drawing.py", line 802 in _draw_geometry
  File "/Applications/ChimeraX-1.6.1.app/Contents/Library/Frameworks/Python.framework/Versions/3.9/lib/python3.9/site-packages/chimerax/graphics/drawing.py", line 775 in draw_self
  File "/Applications/ChimeraX-1.6.1.app/Contents/Library/Frameworks/Python.framework/Versions/3.9/lib/python3.9/site-packages/chimerax/graphics/drawing.py", line 768 in draw
  File "/Applications/ChimeraX-1.6.1.app/Contents/Library/Frameworks/Python.framework/Versions/3.9/lib/python3.9/site-packages/chimerax/graphics/drawing.py", line 1555 in _draw_multiple
  File "/Applications/ChimeraX-1.6.1.app/Contents/Library/Frameworks/Python.framework/Versions/3.9/lib/python3.9/site-packages/chimerax/graphics/drawing.py", line 1542 in draw_opaque
  File "/Applications/ChimeraX-1.6.1.app/Contents/Library/Frameworks/Python.framework/Versions/3.9/lib/python3.9/site-packages/chimerax/graphics/view.py", line 247 in _draw_scene
  File "/Applications/ChimeraX-1.6.1.app/Contents/Library/Frameworks/Python.framework/Versions/3.9/lib/python3.9/site-packages/chimerax/graphics/view.py", line 177 in draw
  File "/Applications/ChimeraX-1.6.1.app/Contents/Library/Frameworks/Python.framework/Versions/3.9/lib/python3.9/site-packages/chimerax/core/updateloop.py", line 73 in draw_new_frame
  File "/Applications/ChimeraX-1.6.1.app/Contents/Library/Frameworks/Python.framework/Versions/3.9/lib/python3.9/site-packages/chimerax/core/updateloop.py", line 158 in update_graphics_now
  File "/Applications/ChimeraX-1.6.1.app/Contents/Library/Frameworks/Python.framework/Versions/3.9/lib/python3.9/site-packages/chimerax/map/volume_viewer.py", line 2233 in moved_marker_cb
  File "/Applications/ChimeraX-1.6.1.app/Contents/Library/Frameworks/Python.framework/Versions/3.9/lib/python3.9/site-packages/chimerax/map/histogram.py", line 416 in move_marker_cb
  File "/Applications/ChimeraX-1.6.1.app/Contents/Library/Frameworks/Python.framework/Versions/3.9/lib/python3.9/site-packages/chimerax/map/volume_viewer.py", line 2159 in _drag
  File "/Applications/ChimeraX-1.6.1.app/Contents/Library/Frameworks/Python.framework/Versions/3.9/lib/python3.9/site-packages/chimerax/map/volume_viewer.py", line 2162 in mouseReleaseEvent
  File "/Applications/ChimeraX-1.6.1.app/Contents/Library/Frameworks/Python.framework/Versions/3.9/lib/python3.9/site-packages/chimerax/ui/gui.py", line 275 in event_loop
  File "/Applications/ChimeraX-1.6.1.app/Contents/Library/Frameworks/Python.framework/Versions/3.9/lib/python3.9/site-packages/chimerax/core/__main__.py", line 892 in init
  File "/Applications/ChimeraX-1.6.1.app/Contents/Library/Frameworks/Python.framework/Versions/3.9/lib/python3.9/site-packages/chimerax/core/__main__.py", line 1043 in 
  File "/Applications/ChimeraX-1.6.1.app/Contents/Library/Frameworks/Python.framework/Versions/3.9/lib/python3.9/runpy.py", line 87 in _run_code
  File "/Applications/ChimeraX-1.6.1.app/Contents/Library/Frameworks/Python.framework/Versions/3.9/lib/python3.9/runpy.py", line 197 in _run_module_as_main


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Address size fault"},"pc":{"value":6925246964},"far":{"value":0}},"frames":[{"imageOffset":4596,"symbol":"mach_msg2_trap","symbolLocation":8,"imageIndex":241},{"imageOffset":80676,"symbol":"mach_msg2_internal","symbolLocation":80,"imageIndex":241},{"imageOffset":40500,"symbol":"mach_msg_overwrite","symbolLocation":476,"imageIndex":241},{"imageOffset":5496,"symbol":"mach_msg","symbolLocation":24,"imageIndex":241},{"imageOffset":62126188,"imageIndex":181},{"imageOffset":61883116,"imageIndex":181},{"imageOffset":61885944,"imageIndex":181},{"imageOffset":61884816,"imageIndex":181},{"imageOffset":62101416,"imageIndex":181},{"imageOffset":28564,"symbol":"_pthread_start","symbolLocation":136,"imageIndex":242},{"imageOffset":7476,"symbol":"thread_start","symbolLocation":8,"imageIndex":242}]},{"id":2429879,"name":"ThreadPoolForegroundWorker","threadState":{"x":[{"value":268451845},{"value":17179869442},{"value":0},{"value":106854491357184},{"value":0},{"value":106854491357184},{"value":32},{"value":33000},{"value":18446744073709550527},{"value":24879},{"value":0},{"value":1},{"value":24879},{"value":2045},{"value":2577780897},{"value":161},{"value":18446744073709551569},{"value":380928},{"value":0},{"value":33000},{"value":32},{"value":106854491357184},{"value":0},{"value":106854491357184},{"value":13544041824},{"value":0},{"value":17179870466},{"value":17179869442},{"value":1282}],"flavor":"ARM_THREAD_STATE64","lr":{"value":6925323044},"cpsr":{"value":4096},"fp":{"value":13544041168},"sp":{"value":13544041088},"esr":{"value":1442840704,"description":" 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===== Log before crash start =====
UCSF ChimeraX version: 1.6.1 (2023-05-09)  
© 2016-2023 Regents of the University of California. All rights reserved.  

> open
> /Users/ruiliu/Desktop/Villa_lab/Data/Titan_processing/042925_PAO1_DarTG_gp104_KO_KZ_40mpi_warp2/segmentation/segmented_maps/L5_1_3_segmented.cxs

Opened
L5_G1_ts_003_corrected_inv_MemBrain_seg_v10_alpha.ckpt_segmented_erased.mrc as
#2, grid size 511,720,375, pixel 13.1,13.1,13.1, shown at level 0.344, step 1,
values float32  
Opened L5_G1_ts_003_corrected_inv_adj_nucleus_smoothed_genome.mrc as #7, grid
size 470,522,44, pixel 13.1,13.1,13.1, shown at level 0.158, step 1, values
float32  
Opened L5_G1_ts_003_corrected_inv_adj_nucleus_shell2.mrc as #11, grid size
511,720,375, pixel 13.1,13.1,13.1, shown at level 0.000346, step 1, values
float32  
Opened
L5_G1_ts_003_corrected_inv_MemBrain_seg_v10_alpha.ckpt_segmented_erased.mrc
copy as #3, grid size 495,720,328, pixel 13.1,13.1,13.1, shown at level 0.344,
step 1, values float32  
Log from Mon Aug 25 11:03:21 2025UCSF ChimeraX version: 1.6.1 (2023-05-09)  
© 2016-2023 Regents of the University of California. All rights reserved.  

> open
> /Users/ruiliu/Desktop/Villa_lab/Data/Titan_processing/042925_PAO1_DarTG_gp104_KO_KZ_40mpi_warp2/segmentation/segmented_maps/L5_1_3_segmented.cxs

Opened
L5_G1_ts_003_corrected_inv_MemBrain_seg_v10_alpha.ckpt_segmented_erased.mrc as
#2, grid size 511,720,375, pixel 13.1,13.1,13.1, shown at level 0.344, step 1,
values float32  
Opened L5_G1_ts_003_corrected_inv_adj_nucleus_smoothed_genome.mrc as #7, grid
size 470,522,44, pixel 13.1,13.1,13.1, shown at level 0.158, step 1, values
float32  
Opened L5_G1_ts_003_corrected_inv_adj_nucleus_shell2.mrc as #11, grid size
511,720,375, pixel 13.1,13.1,13.1, shown at level 0.000346, step 1, values
float32  
Opened
L5_G1_ts_003_corrected_inv_MemBrain_seg_v10_alpha.ckpt_segmented_erased.mrc
copy as #3, grid size 495,720,328, pixel 13.1,13.1,13.1, shown at level 0.344,
step 1, values float32  
Log from Mon Aug 25 11:01:06 2025UCSF ChimeraX version: 1.6.1 (2023-05-09)  
© 2016-2023 Regents of the University of California. All rights reserved.  

> open
> /Users/ruiliu/Desktop/Villa_lab/Data/Titan_processing/042925_PAO1_DarTG_gp104_KO_KZ_40mpi_warp2/segmentation/segmented_maps/L5_1_3_segmented.cxs

Opened
L5_G1_ts_003_corrected_inv_MemBrain_seg_v10_alpha.ckpt_segmented_erased.mrc as
#2, grid size 511,720,375, pixel 13.1,13.1,13.1, shown at level 0.344, step 1,
values float32  
Opened L5_G1_ts_003_corrected_inv_adj_nucleus_smoothed_genome.mrc as #7, grid
size 470,522,44, pixel 13.1,13.1,13.1, shown at level 0.158, step 1, values
float32  
Opened L5_G1_ts_003_corrected_inv_adj_nucleus_shell2.mrc as #11, grid size
511,720,375, pixel 13.1,13.1,13.1, shown at level 0.000346, step 1, values
float32  
Opened
L5_G1_ts_003_corrected_inv_MemBrain_seg_v10_alpha.ckpt_segmented_erased.mrc
copy as #3, grid size 495,720,328, pixel 13.1,13.1,13.1, shown at level 0.344,
step 1, values float32  
Log from Mon Aug 25 10:38:59 2025UCSF ChimeraX version: 1.6.1 (2023-05-09)  
© 2016-2023 Regents of the University of California. All rights reserved.  

> open
> /Users/ruiliu/Desktop/Villa_lab/Data/Titan_processing/042925_PAO1_DarTG_gp104_KO_KZ_40mpi_warp2/segmentation/segmented_maps/L5_1_3_segmented.cxs

Opened
L5_G1_ts_003_corrected_inv_MemBrain_seg_v10_alpha.ckpt_segmented_erased.mrc as
#2, grid size 511,720,375, pixel 13.1,13.1,13.1, shown at level 0.344, step 1,
values float32  
Opened L5_G1_ts_003_corrected_inv_adj_nucleus_smoothed_genome.mrc as #7, grid
size 470,522,44, pixel 13.1,13.1,13.1, shown at level 0.158, step 1, values
float32  
Opened L5_G1_ts_003_corrected_inv_adj_nucleus_shell2.mrc as #11, grid size
511,720,375, pixel 13.1,13.1,13.1, shown at level 0.000346, step 1, values
float32  
Opened
L5_G1_ts_003_corrected_inv_MemBrain_seg_v10_alpha.ckpt_segmented_erased.mrc
copy as #3, grid size 495,720,328, pixel 13.1,13.1,13.1, shown at level 0.344,
step 1, values float32  
Log from Mon Aug 25 10:32:50 2025UCSF ChimeraX version: 1.6.1 (2023-05-09)  
© 2016-2023 Regents of the University of California. All rights reserved.  

> open
> /Users/ruiliu/Desktop/Villa_lab/Data/Titan_processing/042925_PAO1_DarTG_gp104_KO_KZ_40mpi_warp2/segmentation/segmented_maps/L5_1_3_segmented.cxs

Opened
L5_G1_ts_003_corrected_inv_MemBrain_seg_v10_alpha.ckpt_segmented_erased.mrc as
#2, grid size 511,720,375, pixel 13.1,13.1,13.1, shown at level 0.344, step 1,
values float32  
Opened L5_G1_ts_003_corrected_inv_adj_nucleus_smoothed_genome.mrc as #7, grid
size 470,522,44, pixel 13.1,13.1,13.1, shown at level 0.158, step 1, values
float32  
Opened L5_G1_ts_003_corrected_inv_adj_nucleus_shell2.mrc as #11, grid size
511,720,375, pixel 13.1,13.1,13.1, shown at level 0.000346, step 1, values
float32  
Log from Mon Aug 25 09:55:51 2025UCSF ChimeraX version: 1.6.1 (2023-05-09)  
© 2016-2023 Regents of the University of California. All rights reserved.  

> open
> /Users/ruiliu/Desktop/Villa_lab/Data/Titan_processing/042925_PAO1_DarTG_gp104_KO_KZ_40mpi_warp2/segmentation/segmented_maps/L5_1_3_segmented.cxs

Opened
L5_G1_ts_003_corrected_inv_MemBrain_seg_v10_alpha.ckpt_segmented_erased.mrc as
#2, grid size 511,720,375, pixel 13.1,13.1,13.1, shown at level 0.344, step 1,
values float32  
Opened L5_G1_ts_003_corrected_inv_adj_nucleus_smoothed_genome.mrc as #7, grid
size 470,522,44, pixel 13.1,13.1,13.1, shown at level 0.158, step 1, values
float32  
Opened L5_G1_ts_003_corrected_inv_adj_nucleus_shell2.mrc as #11, grid size
511,720,375, pixel 13.1,13.1,13.1, shown at level 0.000346, step 1, values
float32  
Log from Sun Aug 24 18:58:48 2025 Startup Messages  
---  
warning | Replacing fetcher for 'pdb_nmr' and format NMRSTAR from NMRSTAR
bundle with that from NMRSTAR bundle  
  
UCSF ChimeraX version: 1.6.1 (2023-05-09)  
© 2016-2023 Regents of the University of California. All rights reserved.  
How to cite UCSF ChimeraX  

> open
> /Users/ruiliu/Desktop/Villa_lab/Data/Titan_processing/042925_PAO1_DarTG_gp104_KO_KZ_40mpi_warp2/segmentation/L5_G1_ts_003_corrected_inv_adj_nucleus1.mrc

Opened L5_G1_ts_003_corrected_inv_adj_nucleus1.mrc as #1, grid size
511,720,375, pixel 13.1,13.1,13.1, shown at level 0.5, step 4, values float32  

> open
> /Users/ruiliu/Desktop/Villa_lab/Data/Titan_processing/042925_PAO1_DarTG_gp104_KO_KZ_40mpi_warp2/segmentation/L5_G1_ts_003_corrected_inv_adj_membrane_smoothed.mrc

Opened L5_G1_ts_003_corrected_inv_adj_membrane_smoothed.mrc as #2, grid size
511,720,375, pixel 13.1,13.1,13.1, shown at level 8.71e-05, step 4, values
float32  

> hide #!1 models

> open
> /Users/ruiliu/Desktop/Villa_lab/Data/Titan_processing/042925_PAO1_DarTG_gp104_KO_KZ_40mpi_warp2/segmentation/L5_G1_ts_003_corrected_inv_adj_membrane_smoothed_mask.mrc

Opened L5_G1_ts_003_corrected_inv_adj_membrane_smoothed_mask.mrc as #3, grid
size 511,720,375, pixel 13.1,13.1,13.1, shown at level 5e-05, step 4, values
float32  

> volume #3 step 1

> volume #2 step 1

> hide #!3 models

> volume #2 smooth level 2

Expected keyword "smoothLines", "smoothingFactor", or "smoothingIterations"  

> volume #2 smoothingFactor 2

> volume #2 smoothingFactor 20

> close #3

> open
> /Users/ruiliu/Desktop/Villa_lab/Data/Titan_processing/042925_PAO1_DarTG_gp104_KO_KZ_40mpi_warp2/segmentation/L5_G1_ts_003_corrected_inv_adj_membrane_smoothed_mask.mrc

Opened L5_G1_ts_003_corrected_inv_adj_membrane_smoothed_mask.mrc as #3, grid
size 511,720,375, pixel 13.1,13.1,13.1, shown at level 1, step 4, values
float32  

> volume #3 level 1

> volume #3 step 1

> vop gaussian #3 sdev 3 valueType float32

Opened L5_G1_ts_003_corrected_inv_adj_membrane_smoothed_mask.mrc gaussian as
#4, grid size 511,720,375, pixel 13.1,13.1,13.1, shown at step 1, values
float32  

> volume #4 level 1

> open
> /Users/ruiliu/Desktop/Villa_lab/Data/Titan_processing/042925_PAO1_DarTG_gp104_KO_KZ_40mpi_warp2/segmentation/L5_G1_ts_003_corrected_inv_adj_nucleus_smoothed_map.mrc

Opened L5_G1_ts_003_corrected_inv_adj_nucleus_smoothed_map.mrc as #5, grid
size 511,720,375, pixel 13.1,13.1,13.1, shown at level 0.5, step 4, values
float32  

> close #5

> close #4

> close #3

> vop gaussian #2 sdev 3 valueType float32

Opened L5_G1_ts_003_corrected_inv_adj_membrane_smoothed.mrc gaussian as #3,
grid size 511,720,375, pixel 13.1,13.1,13.1, shown at step 1, values float32  

> vop gaussian #2 sdev 15 valueType float32

Opened L5_G1_ts_003_corrected_inv_adj_membrane_smoothed.mrc gaussian as #4,
grid size 511,720,375, pixel 13.1,13.1,13.1, shown at step 1, values float32  

> close #4

> close #3

> open
> /Users/ruiliu/Desktop/Villa_lab/Data/Titan_processing/042925_PAO1_DarTG_gp104_KO_KZ_40mpi_warp2/segmentation/segmented_maps/L5_G1_ts_003_corrected_inv_adj_nucleus_smoothed_mask.mrc

Opened L5_G1_ts_003_corrected_inv_adj_nucleus_smoothed_mask.mrc as #3, grid
size 511,720,375, pixel 13.1,13.1,13.1, shown at level 1, step 4, values int8  

> volume #3 level 0.9889

> volume #3 step 1

> volume dilate #3 voxels -2

Expected a density maps specifier or a keyword  

> vop dilate #3 result #4 level 0.05 radius 3

> volume dilate #3 result #4 level 0.05 radius 3

Expected a density maps specifier or a keyword  

> vop dilate #2 result #4 level 0.05 radius 3

> volume dilate #2 result #4 level 0.05 radius 3

Expected a density maps specifier or a keyword  

> volume dilate t1 #3 result #4 level 0.05 radius 3

Expected a density maps specifier or a keyword  

> volume dilate t1 #2 result #4 level 0.05 radius 3

Expected a density maps specifier or a keyword  

> vop morphology #3 erode iterations 2

> volume morphology #3 erode iterations 2

Expected a density maps specifier or a keyword  

> vop gaussian #3 sdev 3 valueType float32

Opened L5_G1_ts_003_corrected_inv_adj_nucleus_smoothed_mask.mrc gaussian as
#4, grid size 511,720,375, pixel 13.1,13.1,13.1, shown at step 1, values
float32  

> volume #4 level 1

> vop gaussian #3 sdev 15 valueType float32

Opened L5_G1_ts_003_corrected_inv_adj_nucleus_smoothed_mask.mrc gaussian as
#5, grid size 511,720,375, pixel 13.1,13.1,13.1, shown at step 1, values
float32  

> close #4

> volume #5 level 1

> volume #5 level 0.9815

> scalebar 0.9 #4

Expected a keyword  

> close #3

> open
> /Users/ruiliu/Desktop/Villa_lab/Data/Titan_processing/042925_PAO1_DarTG_gp104_KO_KZ_40mpi_warp2/segmentation/L5_1_3_nucleus_smoothed_mesh.mod

Read IMOD model
/Users/ruiliu/Desktop/Villa_lab/Data/Titan_processing/042925_PAO1_DarTG_gp104_KO_KZ_40mpi_warp2/segmentation/L5_1_3_nucleus_smoothed_mesh.mod,
pixel size 1.308, 2 surfaces  

> hide #3.1 models

> show #3.1 models

> hide #3.2 models

> show #3.2 models

> hide #3.2 models

> scalebar 0.9 #3.1

Expected a keyword  

> hide #!3 models

> volume #5 level 0.3845

> volume #5 level 0.09612

> save
> /Users/ruiliu/Desktop/Villa_lab/Data/Titan_processing/042925_PAO1_DarTG_gp104_KO_KZ_40mpi_warp2/segmentation/segmented_maps/L5_G1_ts_003_corrected_inv_adj_nucleus_smoothed_mask_gaussian.mrc
> models #5

> show #!2 models

> close #2

> close #5

> close #1

> open
> /Users/ruiliu/Desktop/Villa_lab/Data/Titan_processing/042925_PAO1_DarTG_gp104_KO_KZ_40mpi_warp2/segmentation/segmented_maps/L5_G1_ts_003_corrected_inv_adj_nucleus_shell.mrc

Opened L5_G1_ts_003_corrected_inv_adj_nucleus_shell.mrc as #1, grid size
472,535,56, pixel 13.1,13.1,13.1, shown at level 6.36e-06, step 1, values
float32  

> volume #1 level 0.001892

> volume #1 level 0.0005

[Repeated 1 time(s)]

> volume #1 level 0.002385

> volume #1 level 1e-06

> vop gaussian #1 sdev 10 valueType float32

Opened L5_G1_ts_003_corrected_inv_adj_nucleus_shell.mrc gaussian as #2, grid
size 472,535,56, pixel 13.1,13.1,13.1, shown at step 1, values float32  

> volume #2 level 7.351e-06

> close #2

> volume #1 sdLevel 2

> volume #1 sdLevel 0.000000001

> volume #1 style image

> volume #1 style surface

> open
> /Users/ruiliu/Desktop/Villa_lab/Data/Titan_processing/042925_PAO1_DarTG_gp104_KO_KZ_40mpi_warp2/segmentation/segmented_maps/L5_G1_ts_003_corrected_inv_MemBrain_seg_v10_alpha.ckpt_segmented_erased.mrc

Opened
L5_G1_ts_003_corrected_inv_MemBrain_seg_v10_alpha.ckpt_segmented_erased.mrc as
#2, grid size 511,720,375, pixel 13.1,13.1,13.1, shown at level 0.125, step 4,
values float32  

> surface dust #2 size 131

> volume #2 level 0.3445

> open
> /Users/ruiliu/Desktop/Villa_lab/Data/Titan_processing/042925_PAO1_DarTG_gp104_KO_KZ_40mpi_warp2/segmentation/L5_1_3_nucleus_smoothed_mesh.mod

Read IMOD model
/Users/ruiliu/Desktop/Villa_lab/Data/Titan_processing/042925_PAO1_DarTG_gp104_KO_KZ_40mpi_warp2/segmentation/L5_1_3_nucleus_smoothed_mesh.mod,
pixel size 1.308, 2 surfaces  

> volume #2 step 1

> hide #4.1 models

> show #4.1 models

> hide #4.2 models

> color #4.1 #baf5b7ff

> open
> /Users/ruiliu/Desktop/Villa_lab/Data/Titan_processing/042925_PAO1_DarTG_gp104_KO_KZ_40mpi_warp2/segmentation/L5_G1_ts_003_corrected.mrc

Opened L5_G1_ts_003_corrected.mrc as #5, grid size 511,720,375, pixel
13.1,13.1,13.1, shown at level 0.158, step 4, values float32  

> volume #5 step 1

> open
> /Users/ruiliu/Desktop/Villa_lab/Data/Titan_processing/042925_PAO1_DarTG_gp104_KO_KZ_40mpi_warp2/segmentation/segmented_maps/L5_G1_ts_003_corrected_inv_adj_nucleus_smoothed_mask.mrc

Opened L5_G1_ts_003_corrected_inv_adj_nucleus_smoothed_mask.mrc as #6, grid
size 511,720,375, pixel 13.1,13.1,13.1, shown at level 1, step 4, values int8  

> volume #6 level 0.9723

> volume #6 step 1

> show #4.2 models

> hide #4.2 models

> hide #!4 models

> volume mask #5 #6

Missing required "surfaces" argument  

> volume mask #5 surfaces #4.1

Opened L5_G1_ts_003_corrected.mrc masked as #7, grid size 470,522,44, pixel
13.1,13.1,13.1, shown at step 1, values float32  

> save
> /Users/ruiliu/Desktop/Villa_lab/Data/Titan_processing/042925_PAO1_DarTG_gp104_KO_KZ_40mpi_warp2/segmentation/segmented_maps/L5_G1_ts_003_corrected_inv_adj_nucleus_smoothed_genome.mrc
> models #7

> close #5

> close #6

> volume #1 change image level -4.615e-05,0 level 0.004963,0.8 level 0.05005,1

> show #!4 models

> vop gaussian #7 sdev 12 valueType float32

Opened L5_G1_ts_003_corrected_inv_adj_nucleus_smoothed_genome.mrc gaussian as
#5, grid size 470,522,44, pixel 13.1,13.1,13.1, shown at step 1, values
float32  

> vop gaussian #7 sdev 20 valueType float32

Opened L5_G1_ts_003_corrected_inv_adj_nucleus_smoothed_genome.mrc gaussian as
#6, grid size 470,522,44, pixel 13.1,13.1,13.1, shown at step 1, values
float32  

> hide #!4 models

> hide #!5 models

> show #!5 models

> close #6

> open
> /Users/ruiliu/Desktop/Villa_lab/Data/Titan_processing/042925_PAO1_DarTG_gp104_KO_KZ_40mpi_warp2/segmentation/L5_G1_ts_003_corrected_inv_adj_nucleus_smoothed_map.mrc

Opened L5_G1_ts_003_corrected_inv_adj_nucleus_smoothed_map.mrc as #6, grid
size 511,720,375, pixel 13.1,13.1,13.1, shown at level 0.5, step 4, values
float32  

> close #6

> open
> /Users/ruiliu/Desktop/Villa_lab/Data/Titan_processing/042925_PAO1_DarTG_gp104_KO_KZ_40mpi_warp2/segmentation/L5_G1_ts_003_corrected_inv_adj_nucleus_smoothed_map.mrc

Opened L5_G1_ts_003_corrected_inv_adj_nucleus_smoothed_map.mrc as #6, grid
size 511,720,375, pixel 13.1,13.1,13.1, shown at level 0.5, step 4, values
float32  

> close #6

> open
> /Users/ruiliu/Desktop/Villa_lab/Data/Titan_processing/042925_PAO1_DarTG_gp104_KO_KZ_40mpi_warp2/segmentation/L5_G1_ts_003_corrected_inv_adj_nucleus1.mrc

Opened L5_G1_ts_003_corrected_inv_adj_nucleus1.mrc as #6, grid size
511,720,375, pixel 13.1,13.1,13.1, shown at level 0.5, step 4, values float32  

> close #6

> open
> /Users/ruiliu/Desktop/Villa_lab/Data/Titan_processing/042925_PAO1_DarTG_gp104_KO_KZ_40mpi_warp2/segmentation/L5_G1_ts_003_corrected_inv_adj_membrane_smoothed.mrc

Opened L5_G1_ts_003_corrected_inv_adj_membrane_smoothed.mrc as #6, grid size
511,720,375, pixel 13.1,13.1,13.1, shown at level 8.71e-05, step 4, values
float32  

> volume #6 step 1

> ui tool show "Map Eraser"

> volume erase #6 center 6106.3,2948,2681.8 radius 1534.3

Opened L5_G1_ts_003_corrected_inv_adj_membrane_smoothed.mrc copy as #9, grid
size 511,720,375, pixel 13.1,13.1,13.1, shown at step 1, values float32  

> volume erase #9 center 6269.2,2582.8,2685.6 radius 1530.4

> volume erase #9 center 6281.1,2546.7,2684.2 radius 1530.4

> volume erase #9 center 7017.4,4100.1,2859.2 radius 1530.4

> volume erase #9 center 7399.1,5010.7,2957.1 radius 1530.4

> volume erase #9 center 6790,5840.6,2956.3 radius 781.66

> volume erase #9 center 6703.8,6055.8,2634.8 radius 668.65

> volume erase #9 center 6638.7,6057.5,2765.2 radius 668.65

> volume erase #9 center 6752,6047.2,2542.9 radius 668.65

> volume erase #9 center 6707.9,6039.2,2472.6 radius 668.65

> volume erase #9 center 4735.7,7205.1,2703.1 radius 668.65

> volume erase #9 center 548.48,4984.7,2522 radius 668.65

> volume erase #9 center 143.74,4281.1,2171.4 radius 668.65

> volume erase #9 center 615.69,4117.4,2552.7 radius 207.19

> volume erase #9 center 681.91,4028.6,2568.7 radius 207.19

> volume erase #9 center 750.44,3942,2552.7 radius 207.19

> volume erase #9 center 6019,1141.4,2392.3 radius 207.19

> volume erase #9 center 6078.1,1064.1,2506.2 radius 207.19

> volume erase #7 center 956.54,3948.8,689.91 radius 204.83

> volume erase #7 center 777.06,4363,2410.8 radius 307.25

> save
> /Users/ruiliu/Desktop/Villa_lab/Data/Titan_processing/042925_PAO1_DarTG_gp104_KO_KZ_40mpi_warp2/segmentation/segmented_maps/L5_G1_ts_003_corrected_inv_adj_nucleus_shell2.mrc
> models #9

> vop gaussian #9 sdev 15 valueType float32

Opened L5_G1_ts_003_corrected_inv_adj_nucleus_shell2.mrc gaussian as #10, grid
size 511,720,375, pixel 13.1,13.1,13.1, shown at step 1, values float32  

> vop gaussian #9 sdev 5 valueType float32

Opened L5_G1_ts_003_corrected_inv_adj_nucleus_shell2.mrc gaussian as #11, grid
size 511,720,375, pixel 13.1,13.1,13.1, shown at step 1, values float32  

> close #10

> save
> /Users/ruiliu/Desktop/Villa_lab/Data/Titan_processing/042925_PAO1_DarTG_gp104_KO_KZ_40mpi_warp2/segmentation/segmented_maps/L5_G1_ts_003_corrected_inv_adj_nucleus_shell2.mrc
> models #11

> artiax start

Using preset: ArtiaX / Artiax Default  
Preset expands to these ChimeraX commands:

    
    
    set bgColor black
    lighting depthCue false
    camera ortho

  

> open
> /Users/ruiliu/Desktop/Villa_lab/Data/Titan_processing/042925_PAO1_DarTG_gp104_KO_KZ_40mpi_warp2/segmentation/run_data.star
> format star

Summary of feedback from opening
/Users/ruiliu/Desktop/Villa_lab/Data/Titan_processing/042925_PAO1_DarTG_gp104_KO_KZ_40mpi_warp2/segmentation/run_data.star  
---  
notes | 15  
['.arrow', '0', '0', '0', '15', '0', '0', '1.0', '4.0']  
  
Opened Particle list run_data.star with 1028 particles.  

> open
> /Users/ruiliu/Desktop/Villa_lab/Data/Titan_processing/042925_PAO1_DarTG_gp104_KO_KZ_40mpi_warp2/segmentation/segmented_maps/PAO1_ribosome_13_08_30.mrc

Opened PAO1_ribosome_13_08_30.mrc as #12, grid size 30,30,30, pixel 13.1,
shown at level 0.0172, step 1, values float32  

> artiax attach #12 toParticleList #10.2.1

Opened PAO1_ribosome_13_08_30.mrc as #10.2.1.1.1, grid size 30,30,30, pixel
13.1, shown at level 0.0172, step 1, values float32  

> volume #10.2.1.1.1 capFaces false

set axis size 196.2  
196.2  
['.arrow', '0', '0', '0', '196.2', '0', '0', '13.08', '52.32']  

> artiax particles #10.2.1 originScaleFactor 13.08

> artiax view xy

> artiax particles #10.2.1 radius 0.1

set axis size 16.82167  
16.82167  
['.arrow', '0', '0', '0', '16.82167', '0', '0', '1.1214446666666666',
'4.485778666666667']  
set axis size 16.0616  
16.0616  
['.arrow', '0', '0', '0', '16.0616', '0', '0', '1.0707733333333331',
'4.2830933333333325']  
set axis size 14.54144  
14.54144  
['.arrow', '0', '0', '0', '14.54144', '0', '0', '0.9694293333333334',
'3.8777173333333335']  
set axis size 12.26122  
12.26122  
['.arrow', '0', '0', '0', '12.26122', '0', '0', '0.8174146666666666',
'3.2696586666666665']  
set axis size 9.22091  
9.22091  
['.arrow', '0', '0', '0', '9.22091', '0', '0', '0.6147273333333333',
'2.458909333333333']  
set axis size 6.94068  
6.94068  
['.arrow', '0', '0', '0', '6.94068', '0', '0', '0.462712', '1.850848']  
set axis size 4.66046  
4.66046  
['.arrow', '0', '0', '0', '4.66046', '0', '0', '0.3106973333333333',
'1.2427893333333333']  
set axis size 0.1  
0.1  
['.arrow', '0', '0', '0', '0.1', '0', '0', '0.006666666666666667',
'0.02666666666666667']  
set axis size 0.1  
0.1  
['.arrow', '0', '0', '0', '0.1', '0', '0', '0.006666666666666667',
'0.02666666666666667']  

> artiax particles #10.2.1 axesSize 0.1

> artiax particles #10.2.1 surfaceLevel 0.00448

> artiax particles #10.2.1 color 100,100,50,100

> artiax particles #10.2.1 color 88,100,40,100

> artiax particles #10.2.1 color 100,80,60,100

fine  
fine  
fine  
fine  

> volume #11 color #b7fbb7

> volume #11 color #c8fbd0

> volume #11 color #c9fbd1

> volume #2 color #70bf79

> volume #7 color #b2b2b2

> save
> /Users/ruiliu/Desktop/Villa_lab/Data/Titan_processing/042925_PAO1_DarTG_gp104_KO_KZ_40mpi_warp2/segmentation/segmented_maps/L5_1_3_segmented.tif
> width 766 height 689 supersample 3

> save
> /Users/ruiliu/Desktop/Villa_lab/Data/Titan_processing/042925_PAO1_DarTG_gp104_KO_KZ_40mpi_warp2/segmentation/segmented_maps/L5_1_3_segmented.cxs
> includeMaps true

Traceback (most recent call last):  
File
"/Applications/ChimeraX-1.6.1.app/Contents/Library/Frameworks/Python.framework/Versions/3.9/lib/python3.9/site-
packages/chimerax/core/session.py", line 262, in discovery  
self.processed[key] = self.process(obj, parents)  
File
"/Applications/ChimeraX-1.6.1.app/Contents/Library/Frameworks/Python.framework/Versions/3.9/lib/python3.9/site-
packages/chimerax/core/session.py", line 299, in process  
return copy_state(data, convert=convert)  
File
"/Applications/ChimeraX-1.6.1.app/Contents/Library/Frameworks/Python.framework/Versions/3.9/lib/python3.9/site-
packages/chimerax/core/state.py", line 219, in copy_state  
return _copy(data)  
File
"/Applications/ChimeraX-1.6.1.app/Contents/Library/Frameworks/Python.framework/Versions/3.9/lib/python3.9/site-
packages/chimerax/core/state.py", line 204, in _copy  
items = [(_copy(k), _copy(v)) for k, v in data.items()]  
File
"/Applications/ChimeraX-1.6.1.app/Contents/Library/Frameworks/Python.framework/Versions/3.9/lib/python3.9/site-
packages/chimerax/core/state.py", line 204, in   
items = [(_copy(k), _copy(v)) for k, v in data.items()]  
File
"/Applications/ChimeraX-1.6.1.app/Contents/Library/Frameworks/Python.framework/Versions/3.9/lib/python3.9/site-
packages/chimerax/core/state.py", line 217, in _copy  
return convert(data)  
File
"/Applications/ChimeraX-1.6.1.app/Contents/Library/Frameworks/Python.framework/Versions/3.9/lib/python3.9/site-
packages/chimerax/core/session.py", line 298, in convert  
return add_obj(obj, parents)  
File
"/Applications/ChimeraX-1.6.1.app/Contents/Library/Frameworks/Python.framework/Versions/3.9/lib/python3.9/site-
packages/chimerax/core/session.py", line 270, in _add_obj  
uid = _UniqueName.from_obj(self.session, obj)  
File
"/Applications/ChimeraX-1.6.1.app/Contents/Library/Frameworks/Python.framework/Versions/3.9/lib/python3.9/site-
packages/chimerax/core/session.py", line 142, in from_obj  
if obj_cls != bundle_info.get_class(obj_cls.__name__, session.logger):  
File
"/Applications/ChimeraX-1.6.1.app/Contents/Library/Frameworks/Python.framework/Versions/3.9/lib/python3.9/site-
packages/chimerax/core/toolshed/info.py", line 476, in get_class  
return f(class_name)  
File "/Users/ruiliu/Library/Application Support/ChimeraX/1.6/site-
packages/chimerax/artiax/__init__.py", line 37, in get_class  
raise ValueError("Unknown class name '%s'" % class_name)  
ValueError: Unknown class name 'MarkerSetPlus'  
  
During handling of the above exception, another exception occurred:  
  
Traceback (most recent call last):  
File
"/Applications/ChimeraX-1.6.1.app/Contents/Library/Frameworks/Python.framework/Versions/3.9/lib/python3.9/site-
packages/chimerax/core/session.py", line 899, in save  
session.save(output, version=version, include_maps=include_maps)  
File
"/Applications/ChimeraX-1.6.1.app/Contents/Library/Frameworks/Python.framework/Versions/3.9/lib/python3.9/site-
packages/chimerax/core/session.py", line 625, in save  
mgr.discovery(self._state_containers)  
File
"/Applications/ChimeraX-1.6.1.app/Contents/Library/Frameworks/Python.framework/Versions/3.9/lib/python3.9/site-
packages/chimerax/core/session.py", line 266, in discovery  
raise ValueError("error processing: %s: %s" % (_obj_stack(parents, obj), e))  
ValueError: error processing: 'attribute registration' ->
 ->
 'M': Unknown class name
'MarkerSetPlus'  
  
ValueError: error processing: 'attribute registration' -> -> 'M': Unknown
class name 'MarkerSetPlus'  
  
File
"/Applications/ChimeraX-1.6.1.app/Contents/Library/Frameworks/Python.framework/Versions/3.9/lib/python3.9/site-
packages/chimerax/core/session.py", line 266, in discovery  
raise ValueError("error processing: %s: %s" % (_obj_stack(parents, obj), e))  
  
See log for complete Python traceback.  
  
Traceback (most recent call last):  
File
"/Applications/ChimeraX-1.6.1.app/Contents/Library/Frameworks/Python.framework/Versions/3.9/lib/python3.9/site-
packages/chimerax/core/session.py", line 262, in discovery  
self.processed[key] = self.process(obj, parents)  
File
"/Applications/ChimeraX-1.6.1.app/Contents/Library/Frameworks/Python.framework/Versions/3.9/lib/python3.9/site-
packages/chimerax/core/session.py", line 299, in process  
return copy_state(data, convert=convert)  
File
"/Applications/ChimeraX-1.6.1.app/Contents/Library/Frameworks/Python.framework/Versions/3.9/lib/python3.9/site-
packages/chimerax/core/state.py", line 219, in copy_state  
return _copy(data)  
File
"/Applications/ChimeraX-1.6.1.app/Contents/Library/Frameworks/Python.framework/Versions/3.9/lib/python3.9/site-
packages/chimerax/core/state.py", line 204, in _copy  
items = [(_copy(k), _copy(v)) for k, v in data.items()]  
File
"/Applications/ChimeraX-1.6.1.app/Contents/Library/Frameworks/Python.framework/Versions/3.9/lib/python3.9/site-
packages/chimerax/core/state.py", line 204, in   
items = [(_copy(k), _copy(v)) for k, v in data.items()]  
File
"/Applications/ChimeraX-1.6.1.app/Contents/Library/Frameworks/Python.framework/Versions/3.9/lib/python3.9/site-
packages/chimerax/core/state.py", line 217, in _copy  
return convert(data)  
File
"/Applications/ChimeraX-1.6.1.app/Contents/Library/Frameworks/Python.framework/Versions/3.9/lib/python3.9/site-
packages/chimerax/core/session.py", line 298, in convert  
return add_obj(obj, parents)  
File
"/Applications/ChimeraX-1.6.1.app/Contents/Library/Frameworks/Python.framework/Versions/3.9/lib/python3.9/site-
packages/chimerax/core/session.py", line 270, in _add_obj  
uid = _UniqueName.from_obj(self.session, obj)  
File
"/Applications/ChimeraX-1.6.1.app/Contents/Library/Frameworks/Python.framework/Versions/3.9/lib/python3.9/site-
packages/chimerax/core/session.py", line 142, in from_obj  
if obj_cls != bundle_info.get_class(obj_cls.__name__, session.logger):  
File
"/Applications/ChimeraX-1.6.1.app/Contents/Library/Frameworks/Python.framework/Versions/3.9/lib/python3.9/site-
packages/chimerax/core/toolshed/info.py", line 476, in get_class  
return f(class_name)  
File "/Users/ruiliu/Library/Application Support/ChimeraX/1.6/site-
packages/chimerax/artiax/__init__.py", line 37, in get_class  
raise ValueError("Unknown class name '%s'" % class_name)  
ValueError: Unknown class name 'MarkerSetPlus'  
  
During handling of the above exception, another exception occurred:  
  
Traceback (most recent call last):  
File
"/Applications/ChimeraX-1.6.1.app/Contents/Library/Frameworks/Python.framework/Versions/3.9/lib/python3.9/site-
packages/chimerax/save_command/dialog.py", line 116, in   
lambda *args, ses=session: show_save_file_dialog(ses), tool_tip="Save output
file",  
File
"/Applications/ChimeraX-1.6.1.app/Contents/Library/Frameworks/Python.framework/Versions/3.9/lib/python3.9/site-
packages/chimerax/save_command/dialog.py", line 127, in show_save_file_dialog  
_dlg.display(session, **kw)  
File
"/Applications/ChimeraX-1.6.1.app/Contents/Library/Frameworks/Python.framework/Versions/3.9/lib/python3.9/site-
packages/chimerax/save_command/dialog.py", line 51, in display  
run(session, cmd)  
File
"/Applications/ChimeraX-1.6.1.app/Contents/Library/Frameworks/Python.framework/Versions/3.9/lib/python3.9/site-
packages/chimerax/core/commands/run.py", line 38, in run  
results = command.run(text, log=log, return_json=return_json)  
File
"/Applications/ChimeraX-1.6.1.app/Contents/Library/Frameworks/Python.framework/Versions/3.9/lib/python3.9/site-
packages/chimerax/core/commands/cli.py", line 2897, in run  
result = ci.function(session, **kw_args)  
File
"/Applications/ChimeraX-1.6.1.app/Contents/Library/Frameworks/Python.framework/Versions/3.9/lib/python3.9/site-
packages/chimerax/save_command/cmd.py", line 75, in cmd_save  
Command(session, registry=registry).run(provider_cmd_text, log=log)  
File
"/Applications/ChimeraX-1.6.1.app/Contents/Library/Frameworks/Python.framework/Versions/3.9/lib/python3.9/site-
packages/chimerax/core/commands/cli.py", line 2897, in run  
result = ci.function(session, **kw_args)  
File
"/Applications/ChimeraX-1.6.1.app/Contents/Library/Frameworks/Python.framework/Versions/3.9/lib/python3.9/site-
packages/chimerax/save_command/cmd.py", line 90, in provider_save  
saver_info.save(session, path, **provider_kw)  
File
"/Applications/ChimeraX-1.6.1.app/Contents/Library/Frameworks/Python.framework/Versions/3.9/lib/python3.9/site-
packages/chimerax/core_formats/__init__.py", line 84, in save  
return cxs_save(session, path, **kw)  
File
"/Applications/ChimeraX-1.6.1.app/Contents/Library/Frameworks/Python.framework/Versions/3.9/lib/python3.9/site-
packages/chimerax/core/session.py", line 899, in save  
session.save(output, version=version, include_maps=include_maps)  
File
"/Applications/ChimeraX-1.6.1.app/Contents/Library/Frameworks/Python.framework/Versions/3.9/lib/python3.9/site-
packages/chimerax/core/session.py", line 625, in save  
mgr.discovery(self._state_containers)  
File
"/Applications/ChimeraX-1.6.1.app/Contents/Library/Frameworks/Python.framework/Versions/3.9/lib/python3.9/site-
packages/chimerax/core/session.py", line 266, in discovery  
raise ValueError("error processing: %s: %s" % (_obj_stack(parents, obj), e))  
ValueError: error processing: 'attribute registration' ->
 ->
 'M': Unknown class name
'MarkerSetPlus'  
  
ValueError: error processing: 'attribute registration' -> -> 'M': Unknown
class name 'MarkerSetPlus'  
  
File
"/Applications/ChimeraX-1.6.1.app/Contents/Library/Frameworks/Python.framework/Versions/3.9/lib/python3.9/site-
packages/chimerax/core/session.py", line 266, in discovery  
raise ValueError("error processing: %s: %s" % (_obj_stack(parents, obj), e))  
  
See log for complete Python traceback.  
  

> close #10

> save
> /Users/ruiliu/Desktop/Villa_lab/Data/Titan_processing/042925_PAO1_DarTG_gp104_KO_KZ_40mpi_warp2/segmentation/segmented_maps/L5_1_3_segmented.cxs
> includeMaps true

> close #5

> close #6

> close #9

> close #1

> save
> /Users/ruiliu/Desktop/Villa_lab/Data/Titan_processing/042925_PAO1_DarTG_gp104_KO_KZ_40mpi_warp2/segmentation/segmented_maps/L5_1_3_segmented.cxs
> includeMaps true

——— End of log from Sun Aug 24 18:58:48 2025 ———

opened ChimeraX session  

> volume #2 color #efecb9

> volume #11 color #c1c3f6

> artiax start

Using preset: ArtiaX / Artiax Default  
Preset expands to these ChimeraX commands:

    
    
    set bgColor black
    lighting depthCue false
    camera ortho

  

> open
> /Users/ruiliu/Desktop/Villa_lab/Data/Titan_processing/042925_PAO1_DarTG_gp104_KO_KZ_40mpi_warp2/segmentation/run_data.star
> format star

Summary of feedback from opening
/Users/ruiliu/Desktop/Villa_lab/Data/Titan_processing/042925_PAO1_DarTG_gp104_KO_KZ_40mpi_warp2/segmentation/run_data.star  
---  
notes | 15  
['.arrow', '0', '0', '0', '15', '0', '0', '1.0', '4.0']  
  
Opened Particle list run_data.star with 1028 particles.  

> open
> /Users/ruiliu/Desktop/Villa_lab/Data/Titan_processing/042925_PAO1_DarTG_gp104_KO_KZ_40mpi_warp2/segmentation/segmented_maps/PAO1_ribosome_13_08_30.mrc

Opened PAO1_ribosome_13_08_30.mrc as #5, grid size 30,30,30, pixel 13.1, shown
at level 0.0172, step 1, values float32  

> artiax attach #5 toParticleList #1.2.1

Opened PAO1_ribosome_13_08_30.mrc as #1.2.1.1.1, grid size 30,30,30, pixel
13.1, shown at level 0.0172, step 1, values float32  

> volume #1.2.1.1.1 capFaces false

set axis size 196.2  
196.2  
['.arrow', '0', '0', '0', '196.2', '0', '0', '13.08', '52.32']  

> artiax particles #1.2.1 originScaleFactor 13.08

> artiax view xy

> artiax particles #1.2.1 radius 0.1

set axis size 11.50114  
11.50114  
['.arrow', '0', '0', '0', '11.50114', '0', '0', '0.7667426666666667',
'3.0669706666666667']  
set axis size 9.98099  
9.98099  
['.arrow', '0', '0', '0', '9.98099', '0', '0', '0.6653993333333333',
'2.6615973333333334']  
set axis size 0.1  
0.1  
['.arrow', '0', '0', '0', '0.1', '0', '0', '0.006666666666666667',
'0.02666666666666667']  
set axis size 0.1  
0.1  
['.arrow', '0', '0', '0', '0.1', '0', '0', '0.006666666666666667',
'0.02666666666666667']  

> artiax particles #1.2.1 axesSize 0.1

> artiax particles #1.2.1 surfaceLevel 0.00409

fine  
fine  
fine  

> artiax particles #1.2.1 color 100,80,60,100

> artiax particles #1.2.1 color 88,100,40,100

> artiax particles #1.2.1 color 0,36,19,100

> artiax particles #1.2.1 color 0,46,86,100

> artiax particles #1.2.1 color 50,50,50,100

> artiax particles #1.2.1 color 26,40,0,100

> artiax particles #1.2.1 color 30,0,36,100

> artiax particles #1.2.1 color 0,36,19,100

> artiax particles #1.2.1 color 0,60,56,100

> artiax particles #1.2.1 color 0,36,19,100

[Repeated 1 time(s)]

> artiax particles #1.2.1 color 44,75,47,100

> artiax particles #1.2.1 color 56,76,90,100

> artiax particles #1.2.1 color 90,64,60,100

> artiax particles #1.2.1 color 90,69,67,100

> artiax particles #1.2.1 color 90,71,73,100

> artiax particles #1.2.1 color 90,75,78,100

> save
> /Users/ruiliu/Desktop/Villa_lab/Data/Titan_processing/042925_PAO1_DarTG_gp104_KO_KZ_40mpi_warp2/segmentation/segmented_maps/ribosome_list.em
> partlist #1.2.1

> artiax particles #1.2.1 color 100,80,60,100

> artiax particles #1.2.1 color 100,66,73,100

> artiax particles #1.2.1 color 87,73,76,100

[Repeated 1 time(s)]

> artiax particles #1.2.1 color 87,72,75,100

[Repeated 2 time(s)]

> artiax particles #1.2.1 color 86,72,75,100

[Repeated 1 time(s)]

> artiax particles #1.2.1 color 86,71,75,100

> artiax particles #1.2.1 color 85,71,75,100

> artiax particles #1.2.1 color 85,71,74,100

[Repeated 1 time(s)]

> artiax particles #1.2.1 color 84,70,73,100

> artiax particles #1.2.1 color 83,69,72,100

> artiax particles #1.2.1 color 82,69,71,100

> artiax particles #1.2.1 color 82,68,71,100

> artiax particles #1.2.1 color 81,67,71,100

> artiax particles #1.2.1 color 81,67,70,100

> artiax particles #1.2.1 color 80,67,70,100

> artiax particles #1.2.1 color 80,67,69,100

> artiax particles #1.2.1 color 80,66,69,100

[Repeated 1 time(s)]

> artiax particles #1.2.1 color 79,66,69,100

[Repeated 2 time(s)]

> artiax particles #1.2.1 color 79,65,68,100

> artiax particles #1.2.1 color 78,65,68,100

[Repeated 2 time(s)]

> artiax particles #1.2.1 color 78,65,67,100

> artiax particles #1.2.1 color 78,65,68,100

> artiax particles #1.2.1 color 79,66,69,100

[Repeated 1 time(s)]

> artiax particles #1.2.1 color 80,67,69,100

> artiax particles #1.2.1 color 80,67,70,100

[Repeated 1 time(s)]

> artiax particles #1.2.1 color 81,67,70,100

[Repeated 1 time(s)]

> artiax particles #1.2.1 color 81,67,71,100

> artiax particles #1.2.1 color 81,68,71,100

> artiax particles #1.2.1 color 82,68,71,100

> artiax particles #1.2.1 color 82,69,71,100

> artiax particles #1.2.1 color 82,69,72,100

[Repeated 1 time(s)]

> artiax particles #1.2.1 color 83,69,72,100

[Repeated 3 time(s)]

> artiax particles #1.2.1 color 83,69,73,100

> artiax particles #1.2.1 color 84,69,73,100

> artiax particles #1.2.1 color 84,70,73,100

[Repeated 3 time(s)]

> artiax particles #1.2.1 color 85,71,74,100

> artiax particles #1.2.1 color 86,71,75,100

> artiax particles #1.2.1 color 86,72,75,100

> artiax particles #1.2.1 color 87,72,75,100

[Repeated 2 time(s)]

> artiax particles #1.2.1 color 86,72,75,100

> artiax particles #1.2.1 color 86,70,78,100

> artiax particles #1.2.1 color 86,67,76,100

> artiax particles #1.2.1 color 86,66,68,100

> artiax particles #1.2.1 color 86,66,84,100

> artiax particles #1.2.1 color 86,68,86,100

> artiax particles #1.2.1 color 86,67,81,100

> artiax particles #1.2.1 color 86,66,76,100

> artiax particles #1.2.1 color 84,70,86,100

> artiax particles #1.2.1 color 86,66,86,100

> artiax particles #1.2.1 color 87,66,86,100

> artiax particles #1.2.1 color 89,68,89,100

> artiax particles #1.2.1 color 90,68,89,100

[Repeated 1 time(s)]

> artiax particles #1.2.1 color 90,69,90,100

> artiax particles #1.2.1 color 91,69,90,100

> artiax particles #1.2.1 color 91,69,91,100

[Repeated 3 time(s)]

> artiax particles #1.2.1 color 91,70,91,100

> artiax particles #1.2.1 color 92,70,92,100

[Repeated 1 time(s)]

> artiax particles #1.2.1 color 93,71,93,100

[Repeated 1 time(s)]

> artiax particles #1.2.1 color 94,71,93,100

> artiax particles #1.2.1 color 94,72,94,100

> artiax particles #1.2.1 color 95,72,94,100

[Repeated 2 time(s)]

> artiax particles #1.2.1 color 94,72,94,100

[Repeated 2 time(s)]

> artiax particles #1.2.1 color 94,71,94,100

> artiax particles #1.2.1 color 94,71,93,100

> artiax particles #1.2.1 color 94,71,94,100

> artiax particles #1.2.1 color 94,72,94,100

[Repeated 1 time(s)]

> artiax particles #1.2.1 color 95,72,94,100

[Repeated 1 time(s)]

> artiax particles #1.2.1 color 95,72,95,100

[Repeated 3 time(s)]

> artiax particles #1.2.1 color 95,73,95,100

[Repeated 3 time(s)]

> artiax particles #1.2.1 color 96,73,95,100

> artiax particles #1.2.1 color 96,73,96,100

[Repeated 1 time(s)]

> artiax particles #1.2.1 color 96,74,96,100

> artiax particles #1.2.1 color 97,74,96,100

[Repeated 1 time(s)]

> artiax particles #1.2.1 color 97,74,97,100

> artiax particles #1.2.1 color 98,75,97,100

> artiax particles #1.2.1 color 98,75,98,100

[Repeated 2 time(s)]

> artiax particles #1.2.1 color 99,75,98,100

> artiax particles #1.2.1 color 98,75,98,100

[Repeated 3 time(s)]

> artiax particles #1.2.1 color 98,75,97,100

> artiax particles #1.2.1 color 97,74,97,100

[Repeated 3 time(s)]

> artiax particles #1.2.1 color 97,74,96,100

[Repeated 1 time(s)]

> artiax particles #1.2.1 color 96,74,96,100

> artiax particles #1.2.1 color 96,73,96,100

[Repeated 5 time(s)]

> artiax particles #1.2.1 color 96,73,95,100

[Repeated 1 time(s)]

> artiax particles #1.2.1 color 95,73,95,100

[Repeated 3 time(s)]

> artiax particles #1.2.1 color 97,74,96,100

> artiax particles #1.2.1 color 97,74,97,100

[Repeated 1 time(s)]

> artiax particles #1.2.1 color 98,74,97,100

> artiax particles #1.2.1 color 98,75,97,100

> artiax particles #1.2.1 color 98,75,98,100

[Repeated 5 time(s)]

> artiax particles #1.2.1 color 99,75,98,100

> artiax particles #1.2.1 color 99,76,99,100

[Repeated 2 time(s)]

> artiax particles #1.2.1 color 100,76,99,100

> artiax particles #1.2.1 color 100,76,100,100

[Repeated 1 time(s)]

> artiax particles #1.2.1 color 95,73,95,100

[Repeated 1 time(s)]

> artiax particles #1.2.1 color 96,74,97,100

> artiax particles #1.2.1 color 97,74,97,100

[Repeated 1 time(s)]

> artiax particles #1.2.1 color 98,75,98,100

[Repeated 3 time(s)]

> artiax particles #1.2.1 color 98,75,99,100

> artiax particles #1.2.1 color 99,76,99,100

[Repeated 3 time(s)]

> artiax particles #1.2.1 color 99,76,100,100

> artiax particles #1.2.1 color 100,76,100,100

[Repeated 4 time(s)]

> artiax particles #1.2.1 color 100,87,98,100

> artiax particles #1.2.1 color 96,83,95,100

> artiax particles #1.2.1 color 95,82,93,100

> artiax particles #1.2.1 color 94,82,93,100

[Repeated 1 time(s)]

> artiax particles #1.2.1 color 93,81,92,100

> artiax particles #1.2.1 color 93,80,91,100

[Repeated 1 time(s)]

> artiax particles #1.2.1 color 92,80,91,100

> artiax particles #1.2.1 color 91,79,90,100

[Repeated 1 time(s)]

> artiax particles #1.2.1 color 91,79,89,100

[Repeated 1 time(s)]

> artiax particles #1.2.1 color 90,78,89,100

[Repeated 2 time(s)]

> artiax particles #1.2.1 color 89,78,88,100

[Repeated 1 time(s)]

> artiax particles #1.2.1 color 89,77,88,100

> artiax particles #1.2.1 color 89,77,87,100

[Repeated 1 time(s)]

> artiax particles #1.2.1 color 88,77,87,100

> artiax particles #1.2.1 color 88,76,87,100

> artiax particles #1.2.1 color 88,76,86,100

> artiax particles #1.2.1 color 87,76,86,100

[Repeated 1 time(s)]

> artiax particles #1.2.1 color 87,75,85,100

[Repeated 2 time(s)]

> artiax particles #1.2.1 color 86,75,85,100

[Repeated 4 time(s)]

> artiax particles #1.2.1 color 86,70,85,100

> artiax particles #1.2.1 color 89,72,87,100

> artiax particles #1.2.1 color 89,73,87,100

> artiax particles #1.2.1 color 89,73,88,100

[Repeated 3 time(s)]

> artiax particles #1.2.1 color 90,73,88,100

[Repeated 2 time(s)]

> artiax particles #1.2.1 color 90,73,89,100

[Repeated 1 time(s)]

> artiax particles #1.2.1 color 90,74,89,100

> artiax particles #1.2.1 color 91,74,89,100

[Repeated 3 time(s)]

> artiax particles #1.2.1 color 91,75,90,100

[Repeated 1 time(s)]

> artiax particles #1.2.1 color 92,75,90,100

[Repeated 1 time(s)]

> artiax particles #1.2.1 color 92,75,91,100

[Repeated 1 time(s)]

> artiax particles #1.2.1 color 93,75,91,100

[Repeated 1 time(s)]

> artiax particles #1.2.1 color 93,76,91,100

[Repeated 1 time(s)]

> artiax particles #1.2.1 color 93,76,92,100

[Repeated 1 time(s)]

> artiax particles #1.2.1 color 94,76,92,100

> artiax particles #1.2.1 color 93,76,92,100

> artiax particles #1.2.1 color 92,75,91,100

> artiax particles #1.2.1 color 92,75,90,100

> artiax particles #1.2.1 color 91,74,89,100

[Repeated 1 time(s)]

> artiax particles #1.2.1 color 90,73,89,100

> artiax particles #1.2.1 color 89,73,88,100

> artiax particles #1.2.1 color 89,72,87,100

> artiax particles #1.2.1 color 88,72,87,100

> artiax particles #1.2.1 color 88,72,86,100

> artiax particles #1.2.1 color 87,71,86,100

[Repeated 1 time(s)]

> artiax particles #1.2.1 color 87,71,85,100

[Repeated 2 time(s)]

> artiax particles #1.2.1 color 86,70,85,100

[Repeated 2 time(s)]

> artiax particles #1.2.1 color 86,70,84,100

[Repeated 2 time(s)]

> artiax particles #1.2.1 color 85,70,84,100

[Repeated 1 time(s)]

> artiax particles #1.2.1 color 85,69,84,100

> artiax particles #1.2.1 color 85,70,84,100

> artiax particles #1.2.1 color 86,70,85,100

> artiax particles #1.2.1 color 87,71,85,100

> artiax particles #1.2.1 color 88,71,86,100

> artiax particles #1.2.1 color 88,72,86,100

> artiax particles #1.2.1 color 88,72,87,100

> artiax particles #1.2.1 color 89,72,87,100

> artiax particles #1.2.1 color 89,73,87,100

> artiax particles #1.2.1 color 89,73,88,100

[Repeated 1 time(s)]

> artiax particles #1.2.1 color 90,73,88,100

> artiax particles #1.2.1 color 90,73,89,100

> artiax particles #1.2.1 color 90,74,89,100

[Repeated 1 time(s)]

> artiax particles #1.2.1 color 91,74,89,100

> artiax particles #1.2.1 color 91,75,90,100

[Repeated 1 time(s)]

> artiax particles #1.2.1 color 92,75,90,100

[Repeated 2 time(s)]

> artiax particles #1.2.1 color 92,75,91,100

[Repeated 2 time(s)]

> artiax particles #1.2.1 color 93,75,91,100

[Repeated 1 time(s)]

> artiax particles #1.2.1 color 93,76,91,100

[Repeated 2 time(s)]

> artiax particles #1.2.1 color 93,76,92,100

> artiax particles #1.2.1 color 94,76,92,100

> artiax particles #1.2.1 color 94,77,93,100

> artiax particles #1.2.1 color 95,77,93,100

[Repeated 1 time(s)]

> artiax particles #1.2.1 color 95,78,93,100

> artiax particles #1.2.1 color 95,78,94,100

[Repeated 1 time(s)]

> open
> /Users/ruiliu/Desktop/Villa_lab/Data/Titan_processing/042925_PAO1_DarTG_gp104_KO_KZ_40mpi_warp2/segmentation/L5_G1_ts_003_corrected.mrc

Opened L5_G1_ts_003_corrected.mrc as #5, grid size 511,720,375, pixel
13.1,13.1,13.1, shown at level 0.158, step 4, values float32  
Traceback (most recent call last):  
File
"/Applications/ChimeraX-1.6.1.app/Contents/Library/Frameworks/Python.framework/Versions/3.9/lib/python3.9/site-
packages/chimerax/core/triggerset.py", line 138, in invoke  
return self._func(self._name, data)  
File "/Users/ruiliu/Library/Application Support/ChimeraX/1.6/site-
packages/chimerax/artiax/widgets/ModelChooserWidget.py", line 60, in
_update_models  
self._populate()  
File "/Users/ruiliu/Library/Application Support/ChimeraX/1.6/site-
packages/chimerax/artiax/widgets/ModelChooserWidget.py", line 63, in _populate  
self.combo.clear()  
RuntimeError: wrapped C/C++ object of type QComboBox has been deleted  
  
Error processing trigger "add models":  
RuntimeError: wrapped C/C++ object of type QComboBox has been deleted  
  
File "/Users/ruiliu/Library/Application Support/ChimeraX/1.6/site-
packages/chimerax/artiax/widgets/ModelChooserWidget.py", line 63, in _populate  
self.combo.clear()  
  
See log for complete Python traceback.  
  
Traceback (most recent call last):  
File
"/Applications/ChimeraX-1.6.1.app/Contents/Library/Frameworks/Python.framework/Versions/3.9/lib/python3.9/site-
packages/chimerax/core/triggerset.py", line 138, in invoke  
return self._func(self._name, data)  
File "/Users/ruiliu/Library/Application Support/ChimeraX/1.6/site-
packages/chimerax/artiax/widgets/ModelChooserWidget.py", line 60, in
_update_models  
self._populate()  
File "/Users/ruiliu/Library/Application Support/ChimeraX/1.6/site-
packages/chimerax/artiax/widgets/ModelChooserWidget.py", line 63, in _populate  
self.combo.clear()  
RuntimeError: wrapped C/C++ object of type QComboBox has been deleted  
  
Error processing trigger "add models":  
RuntimeError: wrapped C/C++ object of type QComboBox has been deleted  
  
File "/Users/ruiliu/Library/Application Support/ChimeraX/1.6/site-
packages/chimerax/artiax/widgets/ModelChooserWidget.py", line 63, in _populate  
self.combo.clear()  
  
See log for complete Python traceback.  
  

> volume #5 step 1

> volume #5 color #b2b2b2

> ui tool show "Side View"

Traceback (most recent call last):  
File
"/Applications/ChimeraX-1.6.1.app/Contents/Library/Frameworks/Python.framework/Versions/3.9/lib/python3.9/site-
packages/chimerax/core/triggerset.py", line 138, in invoke  
return self._func(self._name, data)  
File "/Users/ruiliu/Library/Application Support/ChimeraX/1.6/site-
packages/chimerax/artiax/widgets/ModelChooserWidget.py", line 60, in
_update_models  
self._populate()  
File "/Users/ruiliu/Library/Application Support/ChimeraX/1.6/site-
packages/chimerax/artiax/widgets/ModelChooserWidget.py", line 63, in _populate  
self.combo.clear()  
RuntimeError: wrapped C/C++ object of type QComboBox has been deleted  
  
Error processing trigger "add models":  
RuntimeError: wrapped C/C++ object of type QComboBox has been deleted  
  
File "/Users/ruiliu/Library/Application Support/ChimeraX/1.6/site-
packages/chimerax/artiax/widgets/ModelChooserWidget.py", line 63, in _populate  
self.combo.clear()  
  
See log for complete Python traceback.  
  
Traceback (most recent call last):  
File
"/Applications/ChimeraX-1.6.1.app/Contents/Library/Frameworks/Python.framework/Versions/3.9/lib/python3.9/site-
packages/chimerax/core/triggerset.py", line 138, in invoke  
return self._func(self._name, data)  
File "/Users/ruiliu/Library/Application Support/ChimeraX/1.6/site-
packages/chimerax/artiax/widgets/ModelChooserWidget.py", line 60, in
_update_models  
self._populate()  
File "/Users/ruiliu/Library/Application Support/ChimeraX/1.6/site-
packages/chimerax/artiax/widgets/ModelChooserWidget.py", line 63, in _populate  
self.combo.clear()  
RuntimeError: wrapped C/C++ object of type QComboBox has been deleted  
  
Error processing trigger "add models":  
RuntimeError: wrapped C/C++ object of type QComboBox has been deleted  
  
File "/Users/ruiliu/Library/Application Support/ChimeraX/1.6/site-
packages/chimerax/artiax/widgets/ModelChooserWidget.py", line 63, in _populate  
self.combo.clear()  
  
See log for complete Python traceback.  
  
Traceback (most recent call last):  
File
"/Applications/ChimeraX-1.6.1.app/Contents/Library/Frameworks/Python.framework/Versions/3.9/lib/python3.9/site-
packages/chimerax/core/triggerset.py", line 138, in invoke  
return self._func(self._name, data)  
File "/Users/ruiliu/Library/Application Support/ChimeraX/1.6/site-
packages/chimerax/artiax/widgets/ModelChooserWidget.py", line 60, in
_update_models  
self._populate()  
File "/Users/ruiliu/Library/Application Support/ChimeraX/1.6/site-
packages/chimerax/artiax/widgets/ModelChooserWidget.py", line 63, in _populate  
self.combo.clear()  
RuntimeError: wrapped C/C++ object of type QComboBox has been deleted  
  
Error processing trigger "add models":  
RuntimeError: wrapped C/C++ object of type QComboBox has been deleted  
  
File "/Users/ruiliu/Library/Application Support/ChimeraX/1.6/site-
packages/chimerax/artiax/widgets/ModelChooserWidget.py", line 63, in _populate  
self.combo.clear()  
  
See log for complete Python traceback.  
  
Traceback (most recent call last):  
File
"/Applications/ChimeraX-1.6.1.app/Contents/Library/Frameworks/Python.framework/Versions/3.9/lib/python3.9/site-
packages/chimerax/core/triggerset.py", line 138, in invoke  
return self._func(self._name, data)  
File "/Users/ruiliu/Library/Application Support/ChimeraX/1.6/site-
packages/chimerax/artiax/widgets/ModelChooserWidget.py", line 60, in
_update_models  
self._populate()  
File "/Users/ruiliu/Library/Application Support/ChimeraX/1.6/site-
packages/chimerax/artiax/widgets/ModelChooserWidget.py", line 63, in _populate  
self.combo.clear()  
RuntimeError: wrapped C/C++ object of type QComboBox has been deleted  
  
Error processing trigger "add models":  
RuntimeError: wrapped C/C++ object of type QComboBox has been deleted  
  
File "/Users/ruiliu/Library/Application Support/ChimeraX/1.6/site-
packages/chimerax/artiax/widgets/ModelChooserWidget.py", line 63, in _populate  
self.combo.clear()  
  
See log for complete Python traceback.  
  
Traceback (most recent call last):  
File
"/Applications/ChimeraX-1.6.1.app/Contents/Library/Frameworks/Python.framework/Versions/3.9/lib/python3.9/site-
packages/chimerax/core/triggerset.py", line 138, in invoke  
return self._func(self._name, data)  
File "/Users/ruiliu/Library/Application Support/ChimeraX/1.6/site-
packages/chimerax/artiax/widgets/ModelChooserWidget.py", line 60, in
_update_models  
self._populate()  
File "/Users/ruiliu/Library/Application Support/ChimeraX/1.6/site-
packages/chimerax/artiax/widgets/ModelChooserWidget.py", line 63, in _populate  
self.combo.clear()  
RuntimeError: wrapped C/C++ object of type QComboBox has been deleted  
  
Error processing trigger "add models":  
RuntimeError: wrapped C/C++ object of type QComboBox has been deleted  
  
File "/Users/ruiliu/Library/Application Support/ChimeraX/1.6/site-
packages/chimerax/artiax/widgets/ModelChooserWidget.py", line 63, in _populate  
self.combo.clear()  
  
See log for complete Python traceback.  
  
Traceback (most recent call last):  
File
"/Applications/ChimeraX-1.6.1.app/Contents/Library/Frameworks/Python.framework/Versions/3.9/lib/python3.9/site-
packages/chimerax/core/triggerset.py", line 138, in invoke  
return self._func(self._name, data)  
File "/Users/ruiliu/Library/Application Support/ChimeraX/1.6/site-
packages/chimerax/artiax/widgets/ModelChooserWidget.py", line 60, in
_update_models  
self._populate()  
File "/Users/ruiliu/Library/Application Support/ChimeraX/1.6/site-
packages/chimerax/artiax/widgets/ModelChooserWidget.py", line 63, in _populate  
self.combo.clear()  
RuntimeError: wrapped C/C++ object of type QComboBox has been deleted  
  
Error processing trigger "add models":  
RuntimeError: wrapped C/C++ object of type QComboBox has been deleted  
  
File "/Users/ruiliu/Library/Application Support/ChimeraX/1.6/site-
packages/chimerax/artiax/widgets/ModelChooserWidget.py", line 63, in _populate  
self.combo.clear()  
  
See log for complete Python traceback.  
  
Traceback (most recent call last):  
File
"/Applications/ChimeraX-1.6.1.app/Contents/Library/Frameworks/Python.framework/Versions/3.9/lib/python3.9/site-
packages/chimerax/core/triggerset.py", line 138, in invoke  
return self._func(self._name, data)  
File "/Users/ruiliu/Library/Application Support/ChimeraX/1.6/site-
packages/chimerax/artiax/widgets/ModelChooserWidget.py", line 60, in
_update_models  
self._populate()  
File "/Users/ruiliu/Library/Application Support/ChimeraX/1.6/site-
packages/chimerax/artiax/widgets/ModelChooserWidget.py", line 63, in _populate  
self.combo.clear()  
RuntimeError: wrapped C/C++ object of type QComboBox has been deleted  
  
Error processing trigger "add models":  
RuntimeError: wrapped C/C++ object of type QComboBox has been deleted  
  
File "/Users/ruiliu/Library/Application Support/ChimeraX/1.6/site-
packages/chimerax/artiax/widgets/ModelChooserWidget.py", line 63, in _populate  
self.combo.clear()  
  
See log for complete Python traceback.  
  
Traceback (most recent call last):  
File
"/Applications/ChimeraX-1.6.1.app/Contents/Library/Frameworks/Python.framework/Versions/3.9/lib/python3.9/site-
packages/chimerax/core/triggerset.py", line 138, in invoke  
return self._func(self._name, data)  
File "/Users/ruiliu/Library/Application Support/ChimeraX/1.6/site-
packages/chimerax/artiax/widgets/ModelChooserWidget.py", line 60, in
_update_models  
self._populate()  
File "/Users/ruiliu/Library/Application Support/ChimeraX/1.6/site-
packages/chimerax/artiax/widgets/ModelChooserWidget.py", line 63, in _populate  
self.combo.clear()  
RuntimeError: wrapped C/C++ object of type QComboBox has been deleted  
  
Error processing trigger "add models":  
RuntimeError: wrapped C/C++ object of type QComboBox has been deleted  
  
File "/Users/ruiliu/Library/Application Support/ChimeraX/1.6/site-
packages/chimerax/artiax/widgets/ModelChooserWidget.py", line 63, in _populate  
self.combo.clear()  
  
See log for complete Python traceback.  
  

> select add #7

3 models selected  

> select subtract #7

Nothing selected  

> select add #5

3 models selected  

> ui mousemode right clip

> ui mousemode right "crop volume"

> volume #2 region 384,0,0,510,719,374

> volume #7 region 0,0,0,469,521,43

> volume #2 region 384,0,0,510,719,327

> volume #2 region 384,0,198,510,719,327

> ui mousemode right "crop volume"

[Repeated 1 time(s)]

> volume #7 region 0,0,1,469,521,43

[Repeated 1 time(s)]

> volume #7 region 0,0,0,469,521,43

[Repeated 3 time(s)]

> volume #7 region 0,0,3,469,521,43

[Repeated 1 time(s)]

> volume #2 region 0,0,198,510,719,327

> volume #7 region 0,0,0,469,521,43

> volume #2 region 16,0,198,510,719,327

> volume #7 region 0,0,0,469,521,43

> volume #7 region 0,0,0,462,521,43

> volume #2 region 16,0,198,510,719,327

[Repeated 1 time(s)]

> volume #2 region 16,0,0,510,719,327

[Repeated 1 time(s)]

> ui mousemode right "crop volume"

> select subtract #5

Nothing selected  

> hide #!5 models

Traceback (most recent call last):  
File
"/Applications/ChimeraX-1.6.1.app/Contents/Library/Frameworks/Python.framework/Versions/3.9/lib/python3.9/site-
packages/chimerax/core/triggerset.py", line 138, in invoke  
return self._func(self._name, data)  
File "/Users/ruiliu/Library/Application Support/ChimeraX/1.6/site-
packages/chimerax/artiax/widgets/ModelChooserWidget.py", line 60, in
_update_models  
self._populate()  
File "/Users/ruiliu/Library/Application Support/ChimeraX/1.6/site-
packages/chimerax/artiax/widgets/ModelChooserWidget.py", line 63, in _populate  
self.combo.clear()  
RuntimeError: wrapped C/C++ object of type QComboBox has been deleted  
  
Error processing trigger "remove models":  
RuntimeError: wrapped C/C++ object of type QComboBox has been deleted  
  
File "/Users/ruiliu/Library/Application Support/ChimeraX/1.6/site-
packages/chimerax/artiax/widgets/ModelChooserWidget.py", line 63, in _populate  
self.combo.clear()  
  
See log for complete Python traceback.  
  
Traceback (most recent call last):  
File
"/Applications/ChimeraX-1.6.1.app/Contents/Library/Frameworks/Python.framework/Versions/3.9/lib/python3.9/site-
packages/chimerax/core/triggerset.py", line 138, in invoke  
return self._func(self._name, data)  
File "/Users/ruiliu/Library/Application Support/ChimeraX/1.6/site-
packages/chimerax/artiax/widgets/ModelChooserWidget.py", line 60, in
_update_models  
self._populate()  
File "/Users/ruiliu/Library/Application Support/ChimeraX/1.6/site-
packages/chimerax/artiax/widgets/ModelChooserWidget.py", line 63, in _populate  
self.combo.clear()  
RuntimeError: wrapped C/C++ object of type QComboBox has been deleted  
  
Error processing trigger "remove models":  
RuntimeError: wrapped C/C++ object of type QComboBox has been deleted  
  
File "/Users/ruiliu/Library/Application Support/ChimeraX/1.6/site-
packages/chimerax/artiax/widgets/ModelChooserWidget.py", line 63, in _populate  
self.combo.clear()  
  
See log for complete Python traceback.  
  

> close #5

Traceback (most recent call last):  
File
"/Applications/ChimeraX-1.6.1.app/Contents/Library/Frameworks/Python.framework/Versions/3.9/lib/python3.9/site-
packages/chimerax/core/triggerset.py", line 138, in invoke  
return self._func(self._name, data)  
File "/Users/ruiliu/Library/Application Support/ChimeraX/1.6/site-
packages/chimerax/artiax/widgets/ModelChooserWidget.py", line 60, in
_update_models  
self._populate()  
File "/Users/ruiliu/Library/Application Support/ChimeraX/1.6/site-
packages/chimerax/artiax/widgets/ModelChooserWidget.py", line 63, in _populate  
self.combo.clear()  
RuntimeError: wrapped C/C++ object of type QComboBox has been deleted  
  
Error processing trigger "remove models":  
RuntimeError: wrapped C/C++ object of type QComboBox has been deleted  
  
File "/Users/ruiliu/Library/Application Support/ChimeraX/1.6/site-
packages/chimerax/artiax/widgets/ModelChooserWidget.py", line 63, in _populate  
self.combo.clear()  
  
See log for complete Python traceback.  
  
Traceback (most recent call last):  
File
"/Applications/ChimeraX-1.6.1.app/Contents/Library/Frameworks/Python.framework/Versions/3.9/lib/python3.9/site-
packages/chimerax/core/triggerset.py", line 138, in invoke  
return self._func(self._name, data)  
File "/Users/ruiliu/Library/Application Support/ChimeraX/1.6/site-
packages/chimerax/artiax/widgets/ModelChooserWidget.py", line 60, in
_update_models  
self._populate()  
File "/Users/ruiliu/Library/Application Support/ChimeraX/1.6/site-
packages/chimerax/artiax/widgets/ModelChooserWidget.py", line 63, in _populate  
self.combo.clear()  
RuntimeError: wrapped C/C++ object of type QComboBox has been deleted  
  
Error processing trigger "remove models":  
RuntimeError: wrapped C/C++ object of type QComboBox has been deleted  
  
File "/Users/ruiliu/Library/Application Support/ChimeraX/1.6/site-
packages/chimerax/artiax/widgets/ModelChooserWidget.py", line 63, in _populate  
self.combo.clear()  
  
See log for complete Python traceback.  
  

> show #!3 models

> hide #!3 models

> show #!4 models

> hide #!4 models

> show #!3 models

> hide #!3 models

> close #3

Traceback (most recent call last):  
File
"/Applications/ChimeraX-1.6.1.app/Contents/Library/Frameworks/Python.framework/Versions/3.9/lib/python3.9/site-
packages/chimerax/core/triggerset.py", line 138, in invoke  
return self._func(self._name, data)  
File "/Users/ruiliu/Library/Application Support/ChimeraX/1.6/site-
packages/chimerax/artiax/widgets/ModelChooserWidget.py", line 60, in
_update_models  
self._populate()  
File "/Users/ruiliu/Library/Application Support/ChimeraX/1.6/site-
packages/chimerax/artiax/widgets/ModelChooserWidget.py", line 63, in _populate  
self.combo.clear()  
RuntimeError: wrapped C/C++ object of type QComboBox has been deleted  
  
Error processing trigger "remove models":  
RuntimeError: wrapped C/C++ object of type QComboBox has been deleted  
  
File "/Users/ruiliu/Library/Application Support/ChimeraX/1.6/site-
packages/chimerax/artiax/widgets/ModelChooserWidget.py", line 63, in _populate  
self.combo.clear()  
  
See log for complete Python traceback.  
  
Traceback (most recent call last):  
File
"/Applications/ChimeraX-1.6.1.app/Contents/Library/Frameworks/Python.framework/Versions/3.9/lib/python3.9/site-
packages/chimerax/core/triggerset.py", line 138, in invoke  
return self._func(self._name, data)  
File "/Users/ruiliu/Library/Application Support/ChimeraX/1.6/site-
packages/chimerax/artiax/widgets/ModelChooserWidget.py", line 60, in
_update_models  
self._populate()  
File "/Users/ruiliu/Library/Application Support/ChimeraX/1.6/site-
packages/chimerax/artiax/widgets/ModelChooserWidget.py", line 63, in _populate  
self.combo.clear()  
RuntimeError: wrapped C/C++ object of type QComboBox has been deleted  
  
Error processing trigger "remove models":  
RuntimeError: wrapped C/C++ object of type QComboBox has been deleted  
  
File "/Users/ruiliu/Library/Application Support/ChimeraX/1.6/site-
packages/chimerax/artiax/widgets/ModelChooserWidget.py", line 63, in _populate  
self.combo.clear()  
  
See log for complete Python traceback.  
  

> show #!4 models

> hide #!4 models

> close #4

Traceback (most recent call last):  
File
"/Applications/ChimeraX-1.6.1.app/Contents/Library/Frameworks/Python.framework/Versions/3.9/lib/python3.9/site-
packages/chimerax/core/triggerset.py", line 138, in invoke  
return self._func(self._name, data)  
File "/Users/ruiliu/Library/Application Support/ChimeraX/1.6/site-
packages/chimerax/artiax/widgets/ModelChooserWidget.py", line 60, in
_update_models  
self._populate()  
File "/Users/ruiliu/Library/Application Support/ChimeraX/1.6/site-
packages/chimerax/artiax/widgets/ModelChooserWidget.py", line 63, in _populate  
self.combo.clear()  
RuntimeError: wrapped C/C++ object of type QComboBox has been deleted  
  
Error processing trigger "remove models":  
RuntimeError: wrapped C/C++ object of type QComboBox has been deleted  
  
File "/Users/ruiliu/Library/Application Support/ChimeraX/1.6/site-
packages/chimerax/artiax/widgets/ModelChooserWidget.py", line 63, in _populate  
self.combo.clear()  
  
See log for complete Python traceback.  
  
Traceback (most recent call last):  
File
"/Applications/ChimeraX-1.6.1.app/Contents/Library/Frameworks/Python.framework/Versions/3.9/lib/python3.9/site-
packages/chimerax/core/triggerset.py", line 138, in invoke  
return self._func(self._name, data)  
File "/Users/ruiliu/Library/Application Support/ChimeraX/1.6/site-
packages/chimerax/artiax/widgets/ModelChooserWidget.py", line 60, in
_update_models  
self._populate()  
File "/Users/ruiliu/Library/Application Support/ChimeraX/1.6/site-
packages/chimerax/artiax/widgets/ModelChooserWidget.py", line 63, in _populate  
self.combo.clear()  
RuntimeError: wrapped C/C++ object of type QComboBox has been deleted  
  
Error processing trigger "remove models":  
RuntimeError: wrapped C/C++ object of type QComboBox has been deleted  
  
File "/Users/ruiliu/Library/Application Support/ChimeraX/1.6/site-
packages/chimerax/artiax/widgets/ModelChooserWidget.py", line 63, in _populate  
self.combo.clear()  
  
See log for complete Python traceback.  
  

> save
> /Users/ruiliu/Desktop/Villa_lab/Data/Titan_processing/042925_PAO1_DarTG_gp104_KO_KZ_40mpi_warp2/segmentation/segmented_maps/L5_1_3_segmented_2.tif
> width 766 height 689 supersample 4

> close #1

Traceback (most recent call last):  
File
"/Applications/ChimeraX-1.6.1.app/Contents/Library/Frameworks/Python.framework/Versions/3.9/lib/python3.9/site-
packages/chimerax/core/triggerset.py", line 138, in invoke  
return self._func(self._name, data)  
File "/Users/ruiliu/Library/Application Support/ChimeraX/1.6/site-
packages/chimerax/artiax/widgets/ModelChooserWidget.py", line 60, in
_update_models  
self._populate()  
File "/Users/ruiliu/Library/Application Support/ChimeraX/1.6/site-
packages/chimerax/artiax/widgets/ModelChooserWidget.py", line 63, in _populate  
self.combo.clear()  
RuntimeError: wrapped C/C++ object of type QComboBox has been deleted  
  
Error processing trigger "remove models":  
RuntimeError: wrapped C/C++ object of type QComboBox has been deleted  
  
File "/Users/ruiliu/Library/Application Support/ChimeraX/1.6/site-
packages/chimerax/artiax/widgets/ModelChooserWidget.py", line 63, in _populate  
self.combo.clear()  
  
See log for complete Python traceback.  
  
Traceback (most recent call last):  
File
"/Applications/ChimeraX-1.6.1.app/Contents/Library/Frameworks/Python.framework/Versions/3.9/lib/python3.9/site-
packages/chimerax/core/triggerset.py", line 138, in invoke  
return self._func(self._name, data)  
File "/Users/ruiliu/Library/Application Support/ChimeraX/1.6/site-
packages/chimerax/artiax/widgets/ModelChooserWidget.py", line 60, in
_update_models  
self._populate()  
File "/Users/ruiliu/Library/Application Support/ChimeraX/1.6/site-
packages/chimerax/artiax/widgets/ModelChooserWidget.py", line 63, in _populate  
self.combo.clear()  
RuntimeError: wrapped C/C++ object of type QComboBox has been deleted  
  
Error processing trigger "remove models":  
RuntimeError: wrapped C/C++ object of type QComboBox has been deleted  
  
File "/Users/ruiliu/Library/Application Support/ChimeraX/1.6/site-
packages/chimerax/artiax/widgets/ModelChooserWidget.py", line 63, in _populate  
self.combo.clear()  
  
See log for complete Python traceback.  
  

> save
> /Users/ruiliu/Desktop/Villa_lab/Data/Titan_processing/042925_PAO1_DarTG_gp104_KO_KZ_40mpi_warp2/segmentation/segmented_maps/L5_1_3_segmented.cxs
> includeMaps true

——— End of log from Mon Aug 25 09:55:51 2025 ———

opened ChimeraX session  

> hide #!7 models

> hide #!11 models

> ui tool show "Map Eraser"

> volume erase #2 center 5667.1,7259.8,2312.9 radius 616.49

Opened
L5_G1_ts_003_corrected_inv_MemBrain_seg_v10_alpha.ckpt_segmented_erased.mrc
copy as #3, grid size 495,720,328, pixel 13.1,13.1,13.1, shown at step 1,
values float32  

> volume erase #3 center 4424.5,7634.3,2929.2 radius 612.14

> save
> /Users/ruiliu/Desktop/Villa_lab/Data/Titan_processing/042925_PAO1_DarTG_gp104_KO_KZ_40mpi_warp2/segmentation/segmented_maps/L5_1_3_segmented.cxs
> includeMaps true

——— End of log from Mon Aug 25 10:32:50 2025 ———

opened ChimeraX session  

> clip off

> ui tool show "Map Eraser"

> volume erase #3 center 3741.5,7517.2,1484.8 radius 536.77

> volume erase #3 center 2880.9,6048.5,2774.4 radius 589.91

> volume erase #3 center 2827.6,5807.5,2843.3 radius 382.65

> surface dust #3 size 131

> volume erase #3 center 1937.6,6100,1933 radius 589.91

> volume erase #3 center 1915.1,6138.2,1944.9 radius 589.91

> volume erase #3 center 764.72,5243.8,2021.2 radius 589.91

> volume erase #3 center 750.61,5219.8,2025.1 radius 589.91

> volume erase #3 center 731.62,5275.5,2030.5 radius 589.91

> volume erase #3 center 748.13,5513.6,1600.7 radius 334.82

> volume erase #3 center 2141.9,4319.3,1645.6 radius 605.86

> clip off

> volume erase #3 center 2092.5,4115.4,1863.6 radius 361.39

> save
> /Users/ruiliu/Desktop/Villa_lab/Data/Titan_processing/042925_PAO1_DarTG_gp104_KO_KZ_40mpi_warp2/segmentation/segmented_maps/L5_1_3_segmented.cxs
> includeMaps true

——— End of log from Mon Aug 25 10:38:59 2025 ———

opened ChimeraX session  

> ui tool show "Map Eraser"

> volume erase #3 center -93.462,3195.5,1777.1 radius 616.49

> volume erase #3 center 1542.6,3100.2,2450.8 radius 616.49

> volume erase #3 center 1875.2,1848.5,2192.6 radius 616.49

> save
> /Users/ruiliu/Desktop/Villa_lab/Data/Titan_processing/042925_PAO1_DarTG_gp104_KO_KZ_40mpi_warp2/segmentation/segmented_maps/L5_1_3_segmented.cxs
> includeMaps true

> volume erase #3 center 3322.9,955.82,2921.5 radius 616.49

> volume erase #3 center 3392.8,849.88,2796.6 radius 510.2

> volume erase #3 center 3239.9,699.69,2483.6 radius 292.3

> volume erase #3 center 4715.7,700.22,2932.7 radius 605.86

> save
> /Users/ruiliu/Desktop/Villa_lab/Data/Titan_processing/042925_PAO1_DarTG_gp104_KO_KZ_40mpi_warp2/segmentation/segmented_maps/L5_1_3_segmented.cxs
> includeMaps true

——— End of log from Mon Aug 25 11:01:06 2025 ———

opened ChimeraX session  

> clip off

> show #!7 models

> show #!11 models

> ui mousemode right "move picked models"

> ui mousemode right "crop volume"

> volume #11 region 16,0,0,510,719,327

> volume #11 region 16,0,0,510,719,374

[Repeated 3 time(s)]

> volume #3 region 0,0,0,494,719,327

> clip off

> volume #3 region all

> save
> /Users/ruiliu/Desktop/Villa_lab/Data/Titan_processing/042925_PAO1_DarTG_gp104_KO_KZ_40mpi_warp2/segmentation/segmented_maps/L5_1_3_segmented.cxs
> includeMaps true

——— End of log from Mon Aug 25 11:03:21 2025 ———

opened ChimeraX session  

> clip volume off

Expected a keyword  

> view

> artiax start

Using preset: ArtiaX / Artiax Default  
Preset expands to these ChimeraX commands:

    
    
    set bgColor black
    lighting depthCue false
    camera ortho

  

> open
> /Users/ruiliu/Desktop/Villa_lab/Data/Titan_processing/042925_PAO1_DarTG_gp104_KO_KZ_40mpi_warp2/segmentation/segmented_maps/ribosome_list.em
> format motl

Summary of feedback from opening
/Users/ruiliu/Desktop/Villa_lab/Data/Titan_processing/042925_PAO1_DarTG_gp104_KO_KZ_40mpi_warp2/segmentation/segmented_maps/ribosome_list.em  
---  
notes | 15  
['.arrow', '0', '0', '0', '15', '0', '0', '1.0', '4.0']  
  
Opened Particle list ribosome_list.em with 845 particles.  

> open
> /Users/ruiliu/Desktop/Villa_lab/Data/Titan_processing/042925_PAO1_DarTG_gp104_KO_KZ_40mpi_warp2/segmentation/segmented_maps/PAO1_ribosome_13_08_30.mrc

Opened PAO1_ribosome_13_08_30.mrc as #4, grid size 30,30,30, pixel 13.1, shown
at level 0.0172, step 1, values float32  

> artiax particles #1.2.1 radius 0.1

set axis size 16.0616  
16.0616  
['.arrow', '0', '0', '0', '16.0616', '0', '0', '1.0707733333333331',
'4.2830933333333325']  
set axis size 15.30152  
15.30152  
['.arrow', '0', '0', '0', '15.30152', '0', '0', '1.0201013333333333',
'4.080405333333333']  
set axis size 14.54144  
14.54144  
['.arrow', '0', '0', '0', '14.54144', '0', '0', '0.9694293333333334',
'3.8777173333333335']  
set axis size 13.78137  
13.78137  
['.arrow', '0', '0', '0', '13.78137', '0', '0', '0.9187580000000001',
'3.6750320000000003']  
set axis size 13.02129  
13.02129  
['.arrow', '0', '0', '0', '13.02129', '0', '0', '0.868086', '3.472344']  
set axis size 12.26122  
12.26122  
['.arrow', '0', '0', '0', '12.26122', '0', '0', '0.8174146666666666',
'3.2696586666666665']  
set axis size 10.74106  
10.74106  
['.arrow', '0', '0', '0', '10.74106', '0', '0', '0.7160706666666666',
'2.8642826666666665']  
set axis size 7.70076  
7.70076  
['.arrow', '0', '0', '0', '7.70076', '0', '0', '0.513384', '2.053536']  
set axis size 3.90038  
3.90038  
['.arrow', '0', '0', '0', '3.90038', '0', '0', '0.26002533333333333',
'1.0401013333333333']  
set axis size 0.1  
0.1  
['.arrow', '0', '0', '0', '0.1', '0', '0', '0.006666666666666667',
'0.02666666666666667']  
set axis size 0.1  
0.1  
['.arrow', '0', '0', '0', '0.1', '0', '0', '0.006666666666666667',
'0.02666666666666667']  

> artiax particles #1.2.1 axesSize 0.1

> artiax attach #4 toParticleList #1.2.1

Opened PAO1_ribosome_13_08_30.mrc as #1.2.1.1.1, grid size 30,30,30, pixel
13.1, shown at level 0.0172, step 1, values float32  

> volume #1.2.1.1.1 capFaces false

set axis size 196.2  
196.2  
['.arrow', '0', '0', '0', '196.2', '0', '0', '13.08', '52.32']  

> artiax particles #1.2.1 originScaleFactor 13.08

> artiax view xy

> artiax particles #1.2.1 surfaceLevel 0.006

> artiax particles #1.2.1 radius 0.1

set axis size 2.38023  
2.38023  
['.arrow', '0', '0', '0', '2.38023', '0', '0', '0.15868200000000002',
'0.6347280000000001']  
set axis size 1.62015  
1.62015  
['.arrow', '0', '0', '0', '1.62015', '0', '0', '0.10801', '0.43204']  
set axis size 7.70076  
7.70076  
['.arrow', '0', '0', '0', '7.70076', '0', '0', '0.513384', '2.053536']  
set axis size 13.78137  
13.78137  
['.arrow', '0', '0', '0', '13.78137', '0', '0', '0.9187580000000001',
'3.6750320000000003']  
set axis size 16.82167  
16.82167  
['.arrow', '0', '0', '0', '16.82167', '0', '0', '1.1214446666666666',
'4.485778666666667']  
set axis size 19.86198  
19.86198  
['.arrow', '0', '0', '0', '19.86198', '0', '0', '1.3241319999999999',
'5.2965279999999995']  
set axis size 23.66236  
23.66236  
['.arrow', '0', '0', '0', '23.66236', '0', '0', '1.5774906666666666',
'6.309962666666666']  
set axis size 25.18251  
25.18251  
['.arrow', '0', '0', '0', '25.18251', '0', '0', '1.678834', '6.715336']  
set axis size 26.70266  
26.70266  
['.arrow', '0', '0', '0', '26.70266', '0', '0', '1.7801773333333335',
'7.120709333333334']  
set axis size 28.98289  
28.98289  
['.arrow', '0', '0', '0', '28.98289', '0', '0', '1.9321926666666667',
'7.728770666666667']  
set axis size 33.54335  
33.54335  
['.arrow', '0', '0', '0', '33.54335', '0', '0', '2.2362233333333332',
'8.944893333333333']  
set axis size 37.34373  
37.34373  
['.arrow', '0', '0', '0', '37.34373', '0', '0', '2.489582', '9.958328']  
set axis size 40.38403  
40.38403  
['.arrow', '0', '0', '0', '40.38403', '0', '0', '2.6922686666666666',
'10.769074666666667']  
set axis size 41.90418  
41.90418  
['.arrow', '0', '0', '0', '41.90418', '0', '0', '2.793612', '11.174448']  
set axis size 42.66426  
42.66426  
['.arrow', '0', '0', '0', '42.66426', '0', '0', '2.844284', '11.377136']  
set axis size 45.70456  
45.70456  
['.arrow', '0', '0', '0', '45.70456', '0', '0', '3.0469706666666667',
'12.187882666666667']  
set axis size 47.22471  
47.22471  
['.arrow', '0', '0', '0', '47.22471', '0', '0', '3.148314', '12.593256']  
set axis size 49.50494  
49.50494  
['.arrow', '0', '0', '0', '49.50494', '0', '0', '3.300329333333333',
'13.201317333333332']  
set axis size 50.26502  
50.26502  
['.arrow', '0', '0', '0', '50.26502', '0', '0', '3.3510013333333335',
'13.404005333333334']  
set axis size 51.0251  
51.0251  
['.arrow', '0', '0', '0', '51.0251', '0', '0', '3.4016733333333335',
'13.606693333333334']  
set axis size 53.30532  
53.30532  
['.arrow', '0', '0', '0', '53.30532', '0', '0', '3.553688', '14.214752']  
set axis size 54.0654  
54.0654  
['.arrow', '0', '0', '0', '54.0654', '0', '0', '3.60436', '14.41744']  
set axis size 54.82548  
54.82548  
['.arrow', '0', '0', '0', '54.82548', '0', '0', '3.655032', '14.620128']  
set axis size 55.58555  
55.58555  
['.arrow', '0', '0', '0', '55.58555', '0', '0', '3.705703333333333',
'14.822813333333333']  
set axis size 54.82548  
54.82548  
['.arrow', '0', '0', '0', '54.82548', '0', '0', '3.655032', '14.620128']  
set axis size 54.0654  
54.0654  
['.arrow', '0', '0', '0', '54.0654', '0', '0', '3.60436', '14.41744']  
set axis size 53.30532  
53.30532  
['.arrow', '0', '0', '0', '53.30532', '0', '0', '3.553688', '14.214752']  
set axis size 48.74487  
48.74487  
['.arrow', '0', '0', '0', '48.74487', '0', '0', '3.2496579999999997',
'12.998631999999999']  
set axis size 38.1038  
38.1038  
['.arrow', '0', '0', '0', '38.1038', '0', '0', '2.5402533333333333',
'10.161013333333333']  
set axis size 33.54335  
33.54335  
['.arrow', '0', '0', '0', '33.54335', '0', '0', '2.2362233333333332',
'8.944893333333333']  
set axis size 28.22281  
28.22281  
['.arrow', '0', '0', '0', '28.22281', '0', '0', '1.8815206666666666',
'7.5260826666666665']  
set axis size 25.94259  
25.94259  
['.arrow', '0', '0', '0', '25.94259', '0', '0', '1.729506', '6.918024']  
set axis size 23.66236  
23.66236  
['.arrow', '0', '0', '0', '23.66236', '0', '0', '1.5774906666666666',
'6.309962666666666']  
set axis size 20.62205  
20.62205  
['.arrow', '0', '0', '0', '20.62205', '0', '0', '1.3748033333333334',
'5.4992133333333335']  
set axis size 11.50114  
11.50114  
['.arrow', '0', '0', '0', '11.50114', '0', '0', '0.7667426666666667',
'3.0669706666666667']  
set axis size 4.66046  
4.66046  
['.arrow', '0', '0', '0', '4.66046', '0', '0', '0.3106973333333333',
'1.2427893333333333']  
set axis size 0.1  
0.1  
['.arrow', '0', '0', '0', '0.1', '0', '0', '0.006666666666666667',
'0.02666666666666667']  
set axis size 0.1  
0.1  
['.arrow', '0', '0', '0', '0.1', '0', '0', '0.006666666666666667',
'0.02666666666666667']  

> artiax particles #1.2.1 axesSize 0.1

> artiax particles #1.2.1 color 94,64,100,100

> artiax particles #1.2.1 color 95,78,94,100

> save
> /Users/ruiliu/Desktop/Villa_lab/Data/Titan_processing/042925_PAO1_DarTG_gp104_KO_KZ_40mpi_warp2/segmentation/segmented_maps/L5_1_3_segmented_3.tif
> width 766 height 689 supersample 4

> artiax particles #1.2.1 color 95,78,94,100

> artiax particles #1.2.1 color 94,94,76,100

> artiax particles #1.2.1 color 94,93,83,100

> artiax particles #1.2.1 color 94,94,88,100

> artiax particles #1.2.1 color 93,94,83,100

> volume #11 color #5161f5

> volume #11 color #2247f5

> volume #11 color #2730f5

> volume #11 color #4147f5

> volume #11 color #585af5

> volume #11 color #3753ef

> volume #3 color #f2d1bd

> volume #3 color #f2cfaa

> volume #3 color #f2d8c7

> volume #3 color #f2c8b3

> volume #3 color #f2baaf

> volume #3 color #f2c6a8

> volume #3 color #f2ca98

> volume #3 color #f2c388

> volume #3 color #f2d0ac

> volume #3 color #f2c588

> volume #3 color #f2c79a

fine  
fine  
fine  
fine  
fine  
fine  
fine  
fine  
fine  
fine  
fine  
fine  
fine  
fine  
fine  
fine  

> volume #7 color #56bebf

> volume #7 color #bfbdbf

> volume #7 color #bfb8b3

> volume #7 color #b2b2b2

> artiax particles #1.2.1 color 93,94,83,100

> artiax particles #1.2.1 color 93,95,79,100

> artiax particles #1.2.1 color 92,95,73,100

> artiax particles #1.2.1 color 93,95,70,100

> artiax particles #1.2.1 color 95,96,71,100

> artiax particles #1.2.1 color 95,97,72,100

> artiax particles #1.2.1 color 96,98,72,100

fine  
fine  
fine  
fine  
fine  

> volume #3 color #efc9ac

> volume #3 color #efbe80

> volume #3 color #efac61

> volume #3 color #efad77

> volume #3 color #efb288

> volume #3 color #efab6d

> volume #3 color #efb07a

> volume #3 color #efc188

> volume #3 color #efc17d

> volume #3 color #efb374

> volume #3 color #efba80

> volume #3 color #efb07a

> volume #3 color #ef9e5f

fine  
fine  
fine  
fine  

> save
> /Users/ruiliu/Desktop/Villa_lab/Data/Titan_processing/042925_PAO1_DarTG_gp104_KO_KZ_40mpi_warp2/segmentation/segmented_maps/ribosome_list.em
> partlist #1.2.1

> save
> /Users/ruiliu/Desktop/Villa_lab/Data/Titan_processing/042925_PAO1_DarTG_gp104_KO_KZ_40mpi_warp2/segmentation/segmented_maps/L5_1_3_segmented_3.tif
> width 833 height 689 supersample 4

> open
> /Users/ruiliu/Desktop/Villa_lab/Data/Titan_processing/042925_PAO1_DarTG_gp104_KO_KZ_40mpi_warp2/segmentation/segmented_maps/PhiKZ_capsid_emd_38845.map

Opened PhiKZ_capsid_emd_38845.map as #4, grid size 1000,1000,1000, pixel 1.6,
shown at step 1, values float32  

> volume #4 level -0.002649,0 level 0.01229,0.8 level 0.02372,1

> volume #4 level -0.002649,0 level 0.003922,0.7412 level 0.02372,1

> close #4

> open
> /Users/ruiliu/Desktop/Villa_lab/Data/Titan_processing/042925_PAO1_DarTG_gp104_KO_KZ_40mpi_warp2/segmentation/segmented_maps/PhiKZ_capsid_emd_38845.map

Opened PhiKZ_capsid_emd_38845.map as #4, grid size 1000,1000,1000, pixel 1.6,
shown at step 1, values float32  

> volume #4 region 0,0,0,999,999,999 step 4

> volume #4 step 1

> vop gaussian #4 sdev 12 valueType float32

Opened PhiKZ_capsid_emd_38845.map gaussian as #5, grid size 1000,1000,1000,
pixel 1.6, shown at step 1, values float32  

> volume #4 change image level -0.002649,0 level 0.01229,0.8 level 0.02372,1

> volume #4 level 0.004696


===== Log before crash end =====

Log:
Startup Messages  
---  
warning | Replacing fetcher for 'pdb_nmr' and format NMRSTAR from NMRSTAR
bundle with that from NMRSTAR bundle  
  
UCSF ChimeraX version: 1.6.1 (2023-05-09)  
© 2016-2023 Regents of the University of California. All rights reserved.  
How to cite UCSF ChimeraX  

> open
> /Users/ruiliu/Desktop/Villa_lab/Data/Titan_processing/042925_PAO1_DarTG_gp104_KO_KZ_40mpi_warp2/segmentation/segmented_maps/PhiKZ_capsid_emd_38845.map

Opened PhiKZ_capsid_emd_38845.map as #1, grid size 1000,1000,1000, pixel 1.6,
shown at step 1, values float32  




OpenGL version: 4.1 Metal - 88.1
OpenGL renderer: Apple M3
OpenGL vendor: Apple

Python: 3.9.11
Locale: UTF-8
Qt version: PyQt6 6.4.2, Qt 6.4.2
Qt runtime version: 6.4.3
Qt platform: cocoa
Hardware:

    Hardware Overview:

      Model Name: MacBook Pro
      Model Identifier: Mac15,3
      Model Number: Z1GM000G9LL/A
      Chip: Apple M3
      Total Number of Cores: 8 (4 performance and 4 efficiency)
      Memory: 24 GB
      System Firmware Version: 10151.121.1
      OS Loader Version: 10151.121.1

Software:

    System Software Overview:

      System Version: macOS 14.5 (23F79)
      Kernel Version: Darwin 23.5.0
      Time since boot: 6 days, 22 hours, 29 minutes

Graphics/Displays:

    Apple M3:

      Chipset Model: Apple M3
      Type: GPU
      Bus: Built-In
      Total Number of Cores: 10
      Vendor: Apple (0x106b)
      Metal Support: Metal 3
      Displays:
        Color LCD:
          Display Type: Built-in Liquid Retina XDR Display
          Resolution: 3024 x 1964 Retina
          Main Display: Yes
          Mirror: Off
          Online: Yes
          Automatically Adjust Brightness: Yes
          Connection Type: Internal


Installed Packages:
    alabaster: 0.7.13
    appdirs: 1.4.4
    appnope: 0.1.3
    asttokens: 2.2.1
    Babel: 2.12.1
    backcall: 0.2.0
    beautifulsoup4: 4.11.2
    blockdiag: 3.0.0
    build: 0.10.0
    certifi: 2021.10.8
    cftime: 1.6.2
    charset-normalizer: 3.1.0
    ChimeraX-AddCharge: 1.5.9.1
    ChimeraX-AddH: 2.2.5
    ChimeraX-AlignmentAlgorithms: 2.0.1
    ChimeraX-AlignmentHdrs: 3.3.1
    ChimeraX-AlignmentMatrices: 2.1
    ChimeraX-Alignments: 2.9.3
    ChimeraX-AlphaFold: 1.0
    ChimeraX-AltlocExplorer: 1.0.3
    ChimeraX-AmberInfo: 1.0
    ChimeraX-Arrays: 1.1
    ChimeraX-ArtiaX: 0.3
    ChimeraX-Atomic: 1.43.10
    ChimeraX-AtomicLibrary: 10.0.6
    ChimeraX-AtomSearch: 2.0.1
    ChimeraX-AxesPlanes: 2.3.2
    ChimeraX-BasicActions: 1.1.2
    ChimeraX-BILD: 1.0
    ChimeraX-BlastProtein: 2.1.2
    ChimeraX-BondRot: 2.0.1
    ChimeraX-BugReporter: 1.0.1
    ChimeraX-BuildStructure: 2.8
    ChimeraX-Bumps: 1.0
    ChimeraX-BundleBuilder: 1.2.2
    ChimeraX-ButtonPanel: 1.0.1
    ChimeraX-CageBuilder: 1.0.1
    ChimeraX-CellPack: 1.0
    ChimeraX-Centroids: 1.3.2
    ChimeraX-ChangeChains: 1.0.2
    ChimeraX-CheckWaters: 1.3.1
    ChimeraX-ChemGroup: 2.0.1
    ChimeraX-Clashes: 2.2.4
    ChimeraX-ColorActions: 1.0.3
    ChimeraX-ColorGlobe: 1.0
    ChimeraX-ColorKey: 1.5.3
    ChimeraX-CommandLine: 1.2.5
    ChimeraX-ConnectStructure: 2.0.1
    ChimeraX-Contacts: 1.0.1
    ChimeraX-Core: 1.6.1
    ChimeraX-CoreFormats: 1.1
    ChimeraX-coulombic: 1.4.2
    ChimeraX-Crosslinks: 1.0
    ChimeraX-Crystal: 1.0
    ChimeraX-CrystalContacts: 1.0.1
    ChimeraX-DataFormats: 1.2.3
    ChimeraX-Dicom: 1.2
    ChimeraX-DistMonitor: 1.4
    ChimeraX-DockPrep: 1.1.1
    ChimeraX-Dssp: 2.0
    ChimeraX-EMDB-SFF: 1.0
    ChimeraX-ESMFold: 1.0
    ChimeraX-FileHistory: 1.0.1
    ChimeraX-FunctionKey: 1.0.1
    ChimeraX-Geometry: 1.3
    ChimeraX-gltf: 1.0
    ChimeraX-Graphics: 1.1.1
    ChimeraX-Hbonds: 2.4
    ChimeraX-Help: 1.2.1
    ChimeraX-HKCage: 1.3
    ChimeraX-IHM: 1.1
    ChimeraX-ImageFormats: 1.2
    ChimeraX-IMOD: 1.0
    ChimeraX-IO: 1.0.1
    ChimeraX-ItemsInspection: 1.0.1
    ChimeraX-Label: 1.1.7
    ChimeraX-ListInfo: 1.1.1
    ChimeraX-Log: 1.1.5
    ChimeraX-LookingGlass: 1.1
    ChimeraX-Maestro: 1.8.2
    ChimeraX-Map: 1.1.4
    ChimeraX-MapData: 2.0
    ChimeraX-MapEraser: 1.0.1
    ChimeraX-MapFilter: 2.0.1
    ChimeraX-MapFit: 2.0
    ChimeraX-MapSeries: 2.1.1
    ChimeraX-Markers: 1.0.1
    ChimeraX-Mask: 1.0.2
    ChimeraX-MatchMaker: 2.0.12
    ChimeraX-MDcrds: 2.6
    ChimeraX-MedicalToolbar: 1.0.2
    ChimeraX-Meeting: 1.0.1
    ChimeraX-MLP: 1.1.1
    ChimeraX-mmCIF: 2.12
    ChimeraX-MMTF: 2.2
    ChimeraX-Modeller: 1.5.9
    ChimeraX-ModelPanel: 1.3.7
    ChimeraX-ModelSeries: 1.0.1
    ChimeraX-Mol2: 2.0
    ChimeraX-Mole: 1.0
    ChimeraX-Morph: 1.0.2
    ChimeraX-MouseModes: 1.2
    ChimeraX-Movie: 1.0
    ChimeraX-Neuron: 1.0
    ChimeraX-Nifti: 1.0
    ChimeraX-NRRD: 1.0
    ChimeraX-Nucleotides: 2.0.3
    ChimeraX-OpenCommand: 1.10.1
    ChimeraX-PDB: 2.7.2
    ChimeraX-PDBBio: 1.0
    ChimeraX-PDBLibrary: 1.0.2
    ChimeraX-PDBMatrices: 1.0
    ChimeraX-PickBlobs: 1.0.1
    ChimeraX-Positions: 1.0
    ChimeraX-PresetMgr: 1.1
    ChimeraX-PubChem: 2.1
    ChimeraX-ReadPbonds: 1.0.1
    ChimeraX-Registration: 1.1.1
    ChimeraX-RemoteControl: 1.0
    ChimeraX-RenderByAttr: 1.1
    ChimeraX-RenumberResidues: 1.1
    ChimeraX-ResidueFit: 1.0.1
    ChimeraX-RestServer: 1.1
    ChimeraX-RNALayout: 1.0
    ChimeraX-RotamerLibMgr: 3.0
    ChimeraX-RotamerLibsDunbrack: 2.0
    ChimeraX-RotamerLibsDynameomics: 2.0
    ChimeraX-RotamerLibsRichardson: 2.0
    ChimeraX-SaveCommand: 1.5.1
    ChimeraX-SchemeMgr: 1.0
    ChimeraX-SDF: 2.0.1
    ChimeraX-Segger: 1.0
    ChimeraX-Segment: 1.0.1
    ChimeraX-SelInspector: 1.0
    ChimeraX-SeqView: 2.8.3
    ChimeraX-Shape: 1.0.1
    ChimeraX-Shell: 1.0.1
    ChimeraX-Shortcuts: 1.1.1
    ChimeraX-ShowSequences: 1.0.1
    ChimeraX-SideView: 1.0.1
    ChimeraX-Smiles: 2.1
    ChimeraX-SmoothLines: 1.0
    ChimeraX-SpaceNavigator: 1.0
    ChimeraX-StdCommands: 1.10.3
    ChimeraX-STL: 1.0.1
    ChimeraX-Storm: 1.0
    ChimeraX-StructMeasure: 1.1.2
    ChimeraX-Struts: 1.0.1
    ChimeraX-Surface: 1.0.1
    ChimeraX-SwapAA: 2.0.1
    ChimeraX-SwapRes: 2.2.1
    ChimeraX-TapeMeasure: 1.0
    ChimeraX-Test: 1.0
    ChimeraX-Toolbar: 1.1.2
    ChimeraX-ToolshedUtils: 1.2.1
    ChimeraX-Topography: 1.0
    ChimeraX-Tug: 1.0.1
    ChimeraX-UI: 1.28.4
    ChimeraX-uniprot: 2.2.2
    ChimeraX-UnitCell: 1.0.1
    ChimeraX-ViewDockX: 1.2
    ChimeraX-VIPERdb: 1.0
    ChimeraX-Vive: 1.1
    ChimeraX-VolumeMenu: 1.0.1
    ChimeraX-VTK: 1.0
    ChimeraX-WavefrontOBJ: 1.0
    ChimeraX-WebCam: 1.0.2
    ChimeraX-WebServices: 1.1.1
    ChimeraX-Zone: 1.0.1
    colorama: 0.4.6
    comm: 0.1.3
    contourpy: 1.0.7
    cxservices: 1.2.2
    cycler: 0.11.0
    Cython: 0.29.33
    debugpy: 1.6.7
    decorator: 5.1.1
    docutils: 0.19
    executing: 1.2.0
    filelock: 3.9.0
    fonttools: 4.39.3
    funcparserlib: 1.0.1
    geomdl: 5.3.1
    grako: 3.16.5
    h5py: 3.8.0
    html2text: 2020.1.16
    idna: 3.4
    ihm: 0.35
    imagecodecs: 2022.2.22
    imagesize: 1.4.1
    importlib-metadata: 6.6.0
    ipykernel: 6.21.1
    ipython: 8.10.0
    ipython-genutils: 0.2.0
    ipywidgets: 8.0.6
    jedi: 0.18.2
    Jinja2: 3.1.2
    jupyter-client: 8.0.2
    jupyter-core: 5.3.0
    jupyterlab-widgets: 3.0.7
    kiwisolver: 1.4.4
    line-profiler: 4.0.2
    lxml: 4.9.2
    lz4: 4.3.2
    MarkupSafe: 2.1.2
    matplotlib: 3.6.3
    matplotlib-inline: 0.1.6
    msgpack: 1.0.4
    nest-asyncio: 1.5.6
    netCDF4: 1.6.2
    networkx: 2.8.8
    nibabel: 5.0.1
    nptyping: 2.5.0
    numexpr: 2.8.4
    numpy: 1.23.5
    openvr: 1.23.701
    packaging: 21.3
    pandas: 2.2.2
    ParmEd: 3.4.3
    parso: 0.8.3
    pep517: 0.13.0
    pexpect: 4.8.0
    pickleshare: 0.7.5
    Pillow: 9.3.0
    pip: 23.0
    pkginfo: 1.9.6
    platformdirs: 3.5.0
    prompt-toolkit: 3.0.38
    psutil: 5.9.4
    ptyprocess: 0.7.0
    pure-eval: 0.2.2
    pyarrow: 17.0.0
    pycollada: 0.7.2
    pydicom: 2.3.0
    Pygments: 2.14.0
    pynrrd: 1.0.0
    PyOpenGL: 3.1.5
    PyOpenGL-accelerate: 3.1.5
    pyparsing: 3.0.9
    pyproject-hooks: 1.0.0
    PyQt6-commercial: 6.4.2
    PyQt6-Qt6: 6.4.3
    PyQt6-sip: 13.4.1
    PyQt6-WebEngine-commercial: 6.4.0
    PyQt6-WebEngine-Qt6: 6.4.3
    python-dateutil: 2.8.2
    pytz: 2023.3
    pyzmq: 25.0.2
    qtconsole: 5.4.0
    QtPy: 2.3.1
    RandomWords: 0.4.0
    requests: 2.28.2
    scipy: 1.9.3
    setuptools: 67.4.0
    setuptools-scm: 7.0.5
    sfftk-rw: 0.7.3
    six: 1.16.0
    snowballstemmer: 2.2.0
    sortedcontainers: 2.4.0
    soupsieve: 2.4.1
    sphinx: 6.1.3
    sphinx-autodoc-typehints: 1.22
    sphinxcontrib-applehelp: 1.0.4
    sphinxcontrib-blockdiag: 3.0.0
    sphinxcontrib-devhelp: 1.0.2
    sphinxcontrib-htmlhelp: 2.0.1
    sphinxcontrib-jsmath: 1.0.1
    sphinxcontrib-qthelp: 1.0.3
    sphinxcontrib-serializinghtml: 1.1.5
    stack-data: 0.6.2
    starfile: 0.5.8
    superqt: 0.6.7
    tables: 3.7.0
    tcia-utils: 1.2.0
    tifffile: 2022.10.10
    tinyarray: 1.2.4
    tomli: 2.0.1
    tornado: 6.3.1
    traitlets: 5.9.0
    typing-extensions: 4.5.0
    tzdata: 2023.3
    urllib3: 1.26.15
    wcwidth: 0.2.6
    webcolors: 1.12
    wheel: 0.38.4
    wheel-filename: 1.4.1
    widgetsnbextension: 4.0.7
    zipp: 3.15.0

Change History (2)

comment:1 by pett, 7 weeks ago

Component: UnassignedGraphics
Description: modified (diff)
Owner: set to Tom Goddard
Platform: all
Project: ChimeraX
Status: newassigned
Summary: ChimeraX bug report submissionCrash in glBufferSubData

comment:2 by Tom Goddard, 7 weeks ago

Resolution: nonchimerax
Status: assignedclosed

Adjusting threshold level of a 4 GB map (10003 float32) graphics driver crashed.

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