The following bug report has been submitted:
Platform: macOS-14.0-arm64-arm-64bit
ChimeraX Version: 1.6.1 (2023-05-09 17:57:07 UTC)
Description
mcopy seems to be completely broken, gives error Unknown command: mcopy
Log:
UCSF ChimeraX version: 1.6.1 (2023-05-09)
© 2016-2023 Regents of the University of California. All rights reserved.
> open "/Users/kdr2001/Dropbox/Krishna Cryo-EM
> data/ASR_manuscript/Figures/miscellaneous/Figure1/apo-Na-NaAsp_GltPh_2.cxs"
> format session
Log from Wed Oct 4 22:55:57 2023UCSF ChimeraX version: 1.6.1 (2023-05-09)
© 2016-2023 Regents of the University of California. All rights reserved.
> open "/Users/kdr2001/Dropbox/Krishna Cryo-EM
> data/ASR_manuscript/Figures/Figure1/apo-Na-NaAsp_GltPh.cxs" format session
Log from Wed Oct 4 22:15:15 2023UCSF ChimeraX version: 1.6.1 (2023-05-09)
© 2016-2023 Regents of the University of California. All rights reserved.
> open "/Users/kdr2001/Dropbox/Krishna Cryo-EM
> data/ASR_manuscript/Figures/Figure1/apo-Na-NaAsp_GltPh.cxs"
Log from Mon Sep 25 21:50:45 2023UCSF ChimeraX version: 1.6.1 (2023-05-09)
© 2016-2023 Regents of the University of California. All rights reserved.
> open "/Users/kdr2001/Dropbox/Krishna Cryo-EM
> data/ASR_manuscript/Figures/S1/S1d.cxs" format session
Log from Tue May 23 19:08:01 2023UCSF ChimeraX version: 1.6.1 (2023-05-09)
© 2016-2023 Regents of the University of California. All rights reserved.
> open "/Users/kdr2001/Dropbox/Krishna Cryo-EM
> data/ASR_manuscript/Figures/S1/S1d.cxs"
Log from Fri May 19 07:41:16 2023UCSF ChimeraX version: 1.6.1 (2023-05-09)
© 2016-2023 Regents of the University of California. All rights reserved.
> open "/Users/kdr2001/Dropbox/Krishna Cryo-EM
> data/ASR_manuscript/Figures/S1/S1d.cxs"
Log from Tue May 16 20:23:40 2023UCSF ChimeraX version: 1.6.1 (2023-05-09)
© 2016-2023 Regents of the University of California. All rights reserved.
> open "/Users/kdr2001/Dropbox/Krishna Cryo-EM
> data/ASR_manuscript/Figures/S1/S1e.cxs" format session
Log from Mon May 15 14:59:45 2023UCSF ChimeraX version: 1.6.1 (2023-05-09)
© 2016-2023 Regents of the University of California. All rights reserved.
How to cite UCSF ChimeraX
> open /Users/kdr2001/Downloads/6x15.pdb format pdb
6x15.pdb title:
Inward-facing state of the glutamate transporter homologue gltph In complex
with L-aspartate and sodium ions [more info...]
Chain information for 6x15.pdb #1
---
Chain | Description
A B C | glutamate transporter homologue gltph
Non-standard residues in 6x15.pdb #1
---
6OU — [(2~{R})-1-[2-azanylethoxy(oxidanyl)phosphoryl]oxy-3-hexadecanoyloxy-
propan-2-yl] (~{Z})-octadec-9-enoate
ASP — aspartic acid
FME — N-formylmethionine
HG — mercury (II) ion
NA — sodium ion
> hide #1/b-c
> hide #1/b-c cartoons
> hide cartoons
> hide atoms
> show #1/a:405
> show #1/a:90-94
> show #1/a:90-94 cartoons
> show #1/a:500-504
> show #1/a:310-314
> show #1/a:310-314 cartoons
> show #1/b cartoons
> ui mousemode right zoom
> show #1/a:344-363 cartoons
> show #1/a:344-363
> hide #1/b cartoons
> view orient
> help help:user
> cartoon style #1/a width 0.4
> hide #1/a:344-363 atoms
> show #1/a:350@o
> hide #1/a:313,314 atoms
> hide #1/a:91,92,94 atoms
> hide #1/a:90,91,94 atoms
> color #1/a:344-363 red
> color #1/a:344-363 dark orange
> color #1/a dark orange
> color #1/a:2-314,405 dark orange
> color #1/a:344-363 red
> color byhetero
> show #1/a:405 cartoons
> show #1/a:349,350,352 atoms
> hide #1/a:349,350,352 cartoons
> color #1/a:344-363 dark orange
> color byhetero
> show #1/a:349,350,352 cartoons
> suppressbackbonedisplay false
Unknown command: suppressbackbonedisplay false
> suppressBackboneDisplay false
Unknown command: suppressBackboneDisplay false
> cartoon style #1/a width 0.4 suppressBackboneDisplay false
Expected a keyword
> cartoon #1/a:349,350,352 suppressBackboneDisplay false
> cartoon #1/a:310 suppressBackboneDisplay false
> color byhetero
> cartoon style #1/a width 0.8\
Invalid "width" argument: Expected a number
> cartoon style #1/a width 0.8
> ui tool show H-Bonds
> hbonds reveal true
1083 hydrogen bonds found
> undo
> ~hbonds
[Repeated 1 time(s)]
> hide atoms
> show #1/a:92,93,310,312,405
> show #1/a:500-504
> show #1/a:349,350,352
> cartoon #1/a:349,350,352 suppressBackboneDisplay false
> show #1/a:310 atoms
> select down
Nothing selected
> cartoon #1/a:310 suppressBackboneDisplay false
> cartoon #1/a:311
> show #1/a:311
> color #1/a dark orange
> color byhetero
> cartoon style #1/a width 0.8
> ui mousemode right select
> select /A:502@NA
1 atom, 1 residue, 1 model selected
> select /A:311@SD
1 atom, 1 residue, 1 model selected
> select clear
> select /A:311@SD
1 atom, 1 residue, 1 model selected
> select /A:502@NA
1 atom, 1 residue, 1 model selected
> select /A:311@SD
1 atom, 1 residue, 1 model selected
> select /A:502@NA
1 atom, 1 residue, 1 model selected
> ui mousemode right distance
> distance /A:502@NA /A:311@SD
Distance between /A NA 502 NA and MET 311 SD: 2.940Å
> ui mousemode right distance
> ui mousemode right select
> select clear
> ui mousemode right label
> label #1/A:502
[Repeated 1 time(s)]
> help help:user
> distance /A:502@NA /A:311@SD color skyblue radius 0.075 dashes 6
Distance already exists; modify distance properties with 'distance style'
> distance style #2 color skyblue radius 0.075 dashes 6
> distance style color skyblue
> distance style dashes 6
> distance style radius 0.075
> hide #2.1 models
> hbonds color skyblue
1083 hydrogen bonds found
> undo
> hbonds hide
Expected a keyword
> hbonds delete
> ui mousemode right label
> label #1/A:502
> hide #2.1 models
> label delete residues
> label #1/A:92
> label delete residues
> ui mousemode right select
> ui mousemode right zoom
> ui mousemode right select
> color sel skyblue
> select add /A:310@O
1 atom, 1 pseudobond, 1 residue, 2 models selected
> select clear
> color sel skyblue
> ui mousemode right zoom
> ui mousemode right select
> color sel skyblue
> save "/Users/kdr2001/Dropbox/Krishna Cryo-EM
> data/ASR_manuscript/Figures/S1/S1d.cxs" includeMaps true
> ui mousemode right zoom
> ui mousemode right pivot
> lighting soft
> graphics silhouettes true
> set bgColor white
> lighting multiShadow 512
> ui mousemode right select
> select clear
> ui tool show "Side View"
> view name 1
> view 1
> save /Users/kdr2001/Desktop/image131.png supersample 3
> save "/Users/kdr2001/Dropbox/Krishna Cryo-EM
> data/ASR_manuscript/Figures/S1/S1e.cxs" includeMaps true
——— End of log from Mon May 15 14:59:45 2023 ———
opened ChimeraX session
> view 1
> hide #1:310,349,350,352 atoms
> undo
> cartoon #1:310,349,350,352 backbonesuppressdisplay true
Expected a keyword
> cartoon #1:310,349,350,352 backboneSuppressDisplay true
Expected a keyword
> cartoon #1:310,349,350,352 suppressbackboneDisplay true
Expected a keyword
> cartoon #1:310,349,350,352 suppressBackboneDisplay true
> view 1
> hide #1:310,349,350,352 atoms
> view 1
> save "/Users/kdr2001/Dropbox/Krishna Cryo-EM
> data/ASR_manuscript/Figures/S1/S1d.cxs" includeMaps true
> show #1:310 atoms
> save "/Users/kdr2001/Dropbox/Krishna Cryo-EM
> data/ASR_manuscript/Figures/S1/S1d.cxs"
——— End of log from Tue May 16 20:23:40 2023 ———
opened ChimeraX session
> open /Users/kdr2001/Downloads/6x12.pdb
6x12.pdb title:
Inward-facing apo-open state of the glutamate transporter homologue gltph
[more info...]
Chain information for 6x12.pdb #3
---
Chain | Description
A | glutamate transporter homologue gltph
Non-standard residues in 6x12.pdb #3
---
6OU — [(2~{R})-1-[2-azanylethoxy(oxidanyl)phosphoryl]oxy-3-hexadecanoyloxy-
propan-2-yl] (~{Z})-octadec-9-enoate
> mmaker #3/a:75-90,225-415
> matchmaker #3/a:75-90,225-415
Missing required "to" argument
> mmaker #3/a:75-90,225-415 to #1/a
Parameters
---
Chain pairing | bb
Alignment algorithm | Needleman-Wunsch
Similarity matrix | BLOSUM-62
SS fraction | 0.3
Gap open (HH/SS/other) | 18/18/6
Gap extend | 1
SS matrix | | | H | S | O
---|---|---|---
H | 6 | -9 | -6
S | | 6 | -6
O | | | 4
Iteration cutoff | 2
Matchmaker 6x15.pdb, chain A (#1) with 6x12.pdb, chain A (#3), sequence
alignment score = 1013
RMSD between 152 pruned atom pairs is 0.680 angstroms; (across all 207 pairs:
2.124)
> hide #3 cartoons
> show #3/a:405,344-363,310-314,90-94
> show #3/a:405,344-363,310-314,90-94 cartoons
> hide #3/a:405,344-363,310-314,90-94 atoms
> shiow #3/a:405,92,93,310,312,311
Unknown command: shiow #3/a:405,92,93,310,312,311
> shiow #3/a:405,92,93,310,312,311 atoms
Unknown command: shiow #3/a:405,92,93,310,312,311 atoms
> show #3/a:405,92,93,310,312,311 atoms
> hide #!2 models
> hide #1.1 models
> color #3 #feb255ff
> color #3 #ffcb8dff
> color #3 #ffe5c6ff
> color byhetero
> view orient
> hide #3/b-c atoms
> hide #3/a atoms
> hide #1/b-c atoms
> hide #1/b-c cartoons
> view orient
> hide #3/a:4,7,501,196,199
> view 1
> save "/Users/kdr2001/Dropbox/Krishna Cryo-EM
> data/ASR_manuscript/Figures/S1/S1d.cxs" includeMaps true
——— End of log from Fri May 19 07:41:16 2023 ———
opened ChimeraX session
> view 1
[Repeated 1 time(s)]
> lighting multiShadow 512
> save /Users/kdr2001/Desktop/image132.png supersample 3
> help help:user
> save /Users/kdr2001/Dropbox/GltPh-Na.tiff supersample 3 width 900 height 900
> save /Users/kdr2001/Dropbox/GltPh-Na.tiff supersample "9\\\" width 900
> height 900
Invalid "supersample" argument: Expected an integer
> save /Users/kdr2001/Dropbox/GltPh-Na.tiff supersample 9 width 900 height 900
> save /Users/kdr2001/Dropbox/GltPh-Na.tiff supersample 18 width 900 height
> 900
> save /Users/kdr2001/Dropbox/GltPh-Na.tiff supersample 9 width 900 height 900
> view 1
> save /Users/kdr2001/Dropbox/GltPh-Na.tiff supersample 9 width 900 height 900
> ui mousemode right distance
> distance #1/A:311@SD #1/A:502@NA
Distance already exists; modify distance properties with 'distance style'
> show #2.1 models
> style
> cartoon style #3 width 0.8
> view 1
> save "/Users/kdr2001/Dropbox/Krishna Cryo-EM
> data/ASR_manuscript/Figures/S1/S1d.cxs" includeMaps true
——— End of log from Tue May 23 19:08:01 2023 ———
opened ChimeraX session
> hide #!3 models
> show #!3 models
> hide #!3 models
> show #!3 models
> hide #!2 models
> open "/Users/kdr2001/Downloads/7ahk (2).pdb"
7ahk (2).pdb title:
Crystal structure of the outward-facing state of the substrate-free Na+-ONLY
bound glutamate transporter homolog gltph [more info...]
Chain information for 7ahk (2).pdb #4
---
Chain | Description | UniProt
A | glutamate transporter homolog | GLT_PYRHO 1-417
Non-standard residues in 7ahk (2).pdb #4
---
BOG — octyl β-D-glucopyranoside (β-octylglucoside; octyl β-D-glucoside; octyl
D-glucoside; octyl glucoside)
GOL — glycerol (glycerin; propane-1,2,3-triol)
LFA — eicosane (lipid fragment)
NA — sodium ion
OLC — (2R)-2,3-dihydroxypropyl (9Z)-octadec-9-enoate (1-oleoyl-R-glycerol)
PO4 — phosphate ion
> mmaker #4/a:75-90,225-425
> matchmaker #4/a:75-90,225-425
Missing required "to" argument
> mmaker #4/a:75-90,225-425 to #1
Parameters
---
Chain pairing | bb
Alignment algorithm | Needleman-Wunsch
Similarity matrix | BLOSUM-62
SS fraction | 0.3
Gap open (HH/SS/other) | 18/18/6
Gap extend | 1
SS matrix | | | H | S | O
---|---|---|---
H | 6 | -9 | -6
S | | 6 | -6
O | | | 4
Iteration cutoff | 2
Matchmaker 6x15.pdb, chain A (#1) with 7ahk (2).pdb, chain A (#4), sequence
alignment score = 1006.3
RMSD between 186 pruned atom pairs is 0.732 angstroms; (across all 211 pairs:
2.593)
> hide #!4 models
> show #!4 models
> hide #!4 models
> show #!4 models
> hide #!4 models
> show #!4 models
> hide #!4 models
> show #!4 models
> hide #!4 models
> show #!4 models
> hide #!4 models
> show #!4 models
> hide #!4 models
> show #!4 models
> hide #4
> hide #4 cartoons
> show #4/a:90-94,405,310-314,344-363
> show #4/a:90-94,405,310-314,344-363 cartoons
[Repeated 1 time(s)]
> view 1
> show #4/a:92,93,405,310-312,
Expected a collection of one of 'atoms', 'bonds', 'cartoons', 'models',
'pbonds', 'pseudobonds', 'ribbons', or 'surfaces' or a keyword
> show #4/a:92,93,405,310-312 atoms
> mmaker #4/a:75-90,225-344,363-425 to #1
Parameters
---
Chain pairing | bb
Alignment algorithm | Needleman-Wunsch
Similarity matrix | BLOSUM-62
SS fraction | 0.3
Gap open (HH/SS/other) | 18/18/6
Gap extend | 1
SS matrix | | | H | S | O
---|---|---|---
H | 6 | -9 | -6
S | | 6 | -6
O | | | 4
Iteration cutoff | 2
Matchmaker 6x15.pdb, chain A (#1) with 7ahk (2).pdb, chain A (#4), sequence
alignment score = 924.6
RMSD between 184 pruned atom pairs is 0.715 angstroms; (across all 193 pairs:
2.006)
> show #4@na atoms
> hide #!4 models
> show #!4 models
> hide #2.1 models
> hide #!3 models
> hide #!1 models
> show #!2 models
> hide #!2 models
> show #!2 models
> hide #!2 models
> hide #4.1 models
> show #!1 models
> show #!3 models
> hide #!3 models
> show #!3 models
> style
> cartoon style #4 1
Expected a keyword
> cartoon style #4 width 1
> cartoon style #1-4 width 1
> cartoon style #1-4 width 0.5
> view 1
> cartoon style #1-4 width 0.75
> hide #!4 models
> show #!4 models
> hide #!4 models
> show #!4 models
> hide #!4 models
> show #!4 models
> hide #!4 models
> show #!4 models
> hide #!4 models
> show #!4 models
> hide #!4 models
> show #!4 models
> hide #!4 models
> show #!4 models
> hide #!4 models
> mmaker #4/a:75-90,225-344,363-425@ca to #1
Parameters
---
Chain pairing | bb
Alignment algorithm | Needleman-Wunsch
Similarity matrix | BLOSUM-62
SS fraction | 0.3
Gap open (HH/SS/other) | 18/18/6
Gap extend | 1
SS matrix | | | H | S | O
---|---|---|---
H | 6 | -9 | -6
S | | 6 | -6
O | | | 4
Iteration cutoff | 2
Matchmaker 6x15.pdb, chain A (#1) with 7ahk (2).pdb, chain A (#4), sequence
alignment score = 924.6
RMSD between 184 pruned atom pairs is 0.715 angstroms; (across all 193 pairs:
2.006)
> show #!4 models
> hide #!4 models
> show #!4 models
> hide #!4 models
> show #!4 models
> hide #!4 models
> show #!4 models
> hide #!4 models
> show #!4 models
> hide #!4 models
> show #!4 models
> hide #!4 models
> show #!4 models
> hide #!4 models
> show #!4 models
> hide #!4 models
> show #!4 models
> hide #!4 models
> show #!4 models
> color #1-4 aluminum
Expected a color or one of 'byatom', 'bychain', 'byelement', 'byhetero',
'byidentity', 'bymodel', 'bynucleotide', 'bypolymer', 'fromatoms',
'fromcartoons', 'fromribbons', or 'random' or a keyword
> color #1 darkgrey
> color #3 darkgrey
> color #4 darkgrey
> color byhetero
> save "/Users/kdr2001/Dropbox/Krishna Cryo-EM
> data/ASR_manuscript/Figures/Figure1/apo-Na-NaAsp_GltPh.cxs" includeMaps true
> hide #!4 models
> show #!4 models
> hide #!3 models
> show #!3 models
> hide #!1 models
> show #!1 models
> hide #!1 models
> hide #!3 models
> hide #!4 models
> show #!4 models
> show #!3 models
> hide #!3 models
> show #!3 models
> hide #!3 models
> show #!3 models
> hide #!3 models
> show #!3 models
> show #!1 models
> hide #!1 models
> show #!1 models
> hide #!1 models
> show #!1 models
> hide #!1 models
> hide #!3 models
> hide #!4 models
> save
Missing or invalid "fileName" argument: Expected a file name
> show #!1 models
> color #1:504 mocha
Expected a color or one of 'byatom', 'bychain', 'byelement', 'byhetero',
'byidentity', 'bymodel', 'bynucleotide', 'bypolymer', 'fromatoms',
'fromcartoons', 'fromribbons', or 'random' or a keyword
> color #1:504 byatom mocha
Expected a collection of one of 'All', 'atoms', 'bonds', 'cartoons', 'labels',
'models', 'pseudobonds', 'ribbons', 'rings', or 'surfaces' or a keyword
> color #1:504 brown
> color #!1 byhetero
> color #1:504 salmon
> color #!1 byhetero
> color #1:504 teal
> color #!1 byhetero
> lighting multiShadow 512
> save /Users/kdr2001/Desktop/image10.png supersample 3
> save /Users/kdr2001/Desktop/NaAsp.png supersample 3 width 900 height 900
> hide #!1 models
> show #!3 models
> save /Users/kdr2001/Desktop/apo.png supersample 3 width 900 height 900
> hide #!3 models
> show #!4 models
> save /Users/kdr2001/Desktop/Na.png supersample 3 width 900 height 900
> view 1
> save /Users/kdr2001/Desktop/Na.png supersample 3 width 900 height 900
> hide #!4 models
> show #!3 models
> save /Users/kdr2001/Desktop/apo.png supersample 3 width 900 height 900
> show #!1 models
> hide #!3 models
> save /Users/kdr2001/Desktop/NaAsp.png supersample 3 width 900 height 900
> save "/Users/kdr2001/Dropbox/Krishna Cryo-EM
> data/ASR_manuscript/Figures/Figure1/apo-Na-NaAsp_GltPh.cxs"
——— End of log from Mon Sep 25 21:50:45 2023 ———
opened ChimeraX session
> show #!3 models
> show #!4 models
> hide #!4 models
> hide #!3 models
> open 4p19
4p19 title:
Closed, apo inward-facing state of the glutamate transporter homologue GltPh
[more info...]
Chain information for 4p19 #5
---
Chain | Description | UniProt
A B C | 425aa long hypothetical proton glutamate symport protein |
O59010_PYRHO 1-417
Non-standard residues in 4p19 #5
---
HG — mercury (II) ion
> color #5 darkgrey
> mmaker #5/a:78-106,225-425
> matchmaker #5/a:78-106,225-425
Missing required "to" argument
> mmaker #5/a:78-106,225-425 to #1
Parameters
---
Chain pairing | bb
Alignment algorithm | Needleman-Wunsch
Similarity matrix | BLOSUM-62
SS fraction | 0.3
Gap open (HH/SS/other) | 18/18/6
Gap extend | 1
SS matrix | | | H | S | O
---|---|---|---
H | 6 | -9 | -6
S | | 6 | -6
O | | | 4
Iteration cutoff | 2
Matchmaker 6x15.pdb, chain A (#1) with 4p19, chain A (#5), sequence alignment
score = 1090
RMSD between 189 pruned atom pairs is 0.906 angstroms; (across all 224 pairs:
1.755)
> hide #!5 models
> hide #5 cartoons
> show #!5 models
> hide #5 atoms
> show #5/a:90-94,405,310-314,344-363
> show #5/a:90-94,405,310-314,344-363 cartoons
> show #5:92-93,405,311-314 atoms
> color #!1,5 byhetero
> show #!4 models
> hide #!4 models
> show #!4 models
> show #!3 models
> hide #!3 models
> show #!3 models
> hide #!3 models
> show #!3 models
> hide #!3 models
> hide #!4 models
> hide #!1 models
> view 1
> save "/Users/kdr2001/Dropbox/Krishna Cryo-EM
> data/ASR_manuscript/Figures/Figure1/apo-Na-NaAsp_GltPh.cxs" includeMaps true
——— End of log from Wed Oct 4 22:15:15 2023 ———
opened ChimeraX session
> view 1
> windowsize 900 900
> ui tool show "Side View"
> hie #5/b-c cartoon
Unknown command: hie #5/b-c cartoon
> hide #5/b-c cartoons
> hide #5/b-c atoms
> view 1
> show #!3 models
> hide #!3 models
> show #!3 models
> hide #!3 models
> show #!3 models
> show #!4 models
> hide #!4 models
> show #!4 models
> hide #!4 models
> show #!4 models
> hide #!3 models
> hide #!4 models
> style #5
> cartoon style #5 1
Expected a keyword
> cartoon style #5 width 1
> show #!4 models
> hide #!4 models
> show #!4 models
> hide #!4 models
> save "/Users/kdr2001/Dropbox/Krishna Cryo-EM
> data/ASR_manuscript/Figures/Figure1/apo-Na-NaAsp_GltPh.cxs"
> open "/Users/kdr2001/Dropbox/Krishna Cryo-EM
> data/ASR_manuscript/Structures_Maps/Node7-apo/J1136.pdb"
> "/Users/kdr2001/Dropbox/Krishna Cryo-EM
> data/ASR_manuscript/Structures_Maps/Node7-apo/J1139.pdb"
Chain information for J1136.pdb #6
---
Chain | Description
B | No description available
Chain information for J1139.pdb #7
---
Chain | Description
B | No description available
> color #6 #00ff8cff
> color #7 #00ff8cff
> mmaker #6,7 to #5/a:78-106,225-425
Parameters
---
Chain pairing | bb
Alignment algorithm | Needleman-Wunsch
Similarity matrix | BLOSUM-62
SS fraction | 0.3
Gap open (HH/SS/other) | 18/18/6
Gap extend | 1
SS matrix | | | H | S | O
---|---|---|---
H | 6 | -9 | -6
S | | 6 | -6
O | | | 4
Iteration cutoff | 2
Matchmaker 4p19, chain A (#5) with J1136.pdb, chain B (#6), sequence alignment
score = 780.1
RMSD between 188 pruned atom pairs is 0.951 angstroms; (across all 216 pairs:
1.642)
Matchmaker 4p19, chain A (#5) with J1139.pdb, chain B (#7), sequence alignment
score = 761.5
RMSD between 195 pruned atom pairs is 0.851 angstroms; (across all 216 pairs:
1.407)
> hide #6-7 cartoons
> hide #6-7 atoms
> show #6-7:377,76-80,288-292,322-341 cartoons
> cartoon style #6-7 width 1
> show #6-7:78-79,377,289-291 atoms
[Repeated 1 time(s)]
> hide H
> color #!5-7 byhetero
> hide #!7 models
> show #!7 models
> hide #!6 models
> show #!6 models
> hide #!7 models
> show #!7 models
> hide #!6 models
> show #!6 models
> hide #!6 models
> show #!6 models
> open "/Users/kdr2001/Dropbox/Krishna Cryo-EM
> data/ASR_manuscript/Structures_Maps/Node7-apo/J1140.pdb"
Chain information for J1140.pdb #8
---
Chain | Description
B | No description available
> mmaker #8 to #5/a:78-106,225-425
Parameters
---
Chain pairing | bb
Alignment algorithm | Needleman-Wunsch
Similarity matrix | BLOSUM-62
SS fraction | 0.3
Gap open (HH/SS/other) | 18/18/6
Gap extend | 1
SS matrix | | | H | S | O
---|---|---|---
H | 6 | -9 | -6
S | | 6 | -6
O | | | 4
Iteration cutoff | 2
Matchmaker 4p19, chain A (#5) with J1140.pdb, chain B (#8), sequence alignment
score = 765.1
RMSD between 166 pruned atom pairs is 0.922 angstroms; (across all 216 pairs:
2.607)
> hide #8 cartoons
> hide #8 atoms
> mmaker #8 to #5/a:78-106,225-425
Parameters
---
Chain pairing | bb
Alignment algorithm | Needleman-Wunsch
Similarity matrix | BLOSUM-62
SS fraction | 0.3
Gap open (HH/SS/other) | 18/18/6
Gap extend | 1
SS matrix | | | H | S | O
---|---|---|---
H | 6 | -9 | -6
S | | 6 | -6
O | | | 4
Iteration cutoff | 2
Matchmaker 4p19, chain A (#5) with J1140.pdb, chain B (#8), sequence alignment
score = 765.1
RMSD between 166 pruned atom pairs is 0.922 angstroms; (across all 216 pairs:
2.607)
> show #8:377,76-80,288-292,322-341 cartoons
> color #8 #00ff8cff
> cartoon style #8 width 1
> show #8:78-79,377,289-291 atoms
> hide H
> color #!5-8 byhetero
> hide #!7 models
> hide #!6 models
> show #!6 models
> hide #!6 models
> show #!6 models
> hide #!6 models
> show #!6 models
> hide #!6 models
> show #!6 models
> hide #!6 models
> show #!6 models
> hide #!6 models
> show #!6 models
> hide #!6 models
> show #!6 models
> hide #!6 models
> show #!6 models
> hide #!6 models
> show #!6 models
> hide #!6 models
> show #!6 models
> hide #!6 models
> show #!6 models
> hide #!6 models
> show #!6 models
> hide #!6 models
> show #!6 models
> hide #!6 models
> show #!6 models
> hide #!6 models
> show #!6 models
> hide #!6 models
> show #!6 models
> hide #!6 models
> show #!6 models
> hide #!6 models
> show #!6 models
> hide #!6 models
> save /Users/kdr2001/Desktop/image20.png supersample 3
> hide #!8 models
> show #!6 models
> save /Users/kdr2001/Desktop/image21.png supersample 3
> hide #!6 models
> show #!7 models
> save /Users/kdr2001/Desktop/4p19_1139.png supersample 3
> hide #!5 models
> show #!4 models
> save /Users/kdr2001/Desktop/7ahk_1139.png supersample 3
> hide #!7 models
> show #!6 models
> save /Users/kdr2001/Desktop/7ahk_1136.png supersample 3
> hide #!6 models
> hide #!4 models
> show #!3 models
> show #!6 models
> save /Users/kdr2001/Desktop/6x12_1136.png supersample 3
> hide #!6 models
> show #!7 models
> save /Users/kdr2001/Desktop/6x12_1139.png supersample 3
> show #!1 models
> hide #!1 models
> show #!8 models
> show #!6 models
> hide #!8 models
> save "/Users/kdr2001/Dropbox/Krishna Cryo-EM
> data/ASR_manuscript/Figures/Figure1/apo-Na-NaAsp_GltPh_2.cxs"
> hide #!3 models
> show #!5 models
> hide #!7 models
> save /Users/kdr2001/Desktop/4p19_1136.png supersample 3
> hide #!6 models
> save /Users/kdr2001/Desktop/4p19.png supersample 3
> hide #!5 models
> show #!4 models
> save /Users/kdr2001/Desktop/7ahk.png supersample 3
> hide #!4 models
> show #!3 models
> save /Users/kdr2001/Desktop/6x12.png supersample 3
> show #!5 models
> hide #!3 models
> SHOW :310 ATOMS
Unknown command: SHOW :310 ATOMS
> show #5:310 atoms
> view 1
> save /Users/kdr2001/Desktop/4p19.png supersample 3
> show #!6 models
> save /Users/kdr2001/Desktop/4p19_1136.png supersample 3
> hide #!6 models
> show #!7 models
> hide #!7 models
> show #!7 models
> show #!6 models
> hide #!7 models
> hide #!6 models
> hide #!5 models
> show #!6 models
> show #6-7:288
> hide H
> hide #!6 models
> show #!6 models
> show #!1 models
> hide #!1 models
> show #!1 models
> hide #!1 models
> show #!3 models
> hide #!3 models
> show #!4 models
> hide #!6 models
> show #!6 models
> hide #!6 models
> show #!8 models
> hide #!8 models
> show #!5 models
> view 1
> hide #!5 models
> hide #!4 models
> show #!5 models
> show #!6 models
> hide #1-5:314
> show #!3 models
> show #!4 models
> hide #!3 models
> hide #!4 models
> hide #!5 models
> view 1
> save "/Users/kdr2001/Dropbox/Krishna Cryo-EM
> data/ASR_manuscript/Figures/Figure1/apo-Na-NaAsp_GltPh_2.cxs"
——— End of log from Wed Oct 4 22:55:57 2023 ———
opened ChimeraX session
> show #!7 models
> show #!8 models
> show #!3 models
> hide #!3 models
> show #!1 models
> hide #!1 models
> show #!1 models
> show #!4 models
> close #5
> close #3
> show #!2 models
> hide #!2 models
> close #2
> open "/Users/kdr2001/Library/CloudStorage/OneDrive-med.cornell.edu/Olga
> Boudker - Olga Boudker's files/Krishna-Olga-ASR/Farideh's map
> model/NewClasses/ofs.pdb"
ofs.pdb title:
Gltph In complex with L-aspartate and sodium ions In outward-facing state
[more info...]
Chain information for ofs.pdb #2
---
Chain | Description
C | No description available
> mmaker #2 to #4:225-309,364-425
Parameters
---
Chain pairing | bb
Alignment algorithm | Needleman-Wunsch
Similarity matrix | BLOSUM-62
SS fraction | 0.3
Gap open (HH/SS/other) | 18/18/6
Gap extend | 1
SS matrix | | | H | S | O
---|---|---|---
H | 6 | -9 | -6
S | | 6 | -6
O | | | 4
Iteration cutoff | 2
Matchmaker 7ahk (2).pdb, chain A (#4) with ofs.pdb, chain C (#2), sequence
alignment score = 700.3
RMSD between 125 pruned atom pairs is 0.649 angstroms; (across all 138 pairs:
1.320)
> mcopy
Unknown command: mcopy
> mcopy #4 toAtoms #2
Unknown command: mcopy #4 toAtoms #2
> toolshed show
OpenGL version: 4.1 Metal - 86
OpenGL renderer: Apple M1
OpenGL vendor: Apple
Python: 3.9.11
Locale: UTF-8
Qt version: PyQt6 6.4.2, Qt 6.4.2
Qt runtime version: 6.4.3
Qt platform: cocoa
Hardware:
Hardware Overview:
Model Name: MacBook Pro
Model Identifier: MacBookPro17,1
Model Number: Z11C000EMLL/A
Chip: Apple M1
Total Number of Cores: 8 (4 performance and 4 efficiency)
Memory: 16 GB
System Firmware Version: 10151.1.1
OS Loader Version: 10151.1.1
Software:
System Software Overview:
System Version: macOS 14.0 (23A344)
Kernel Version: Darwin 23.0.0
Time since boot: 11 days, 12 hours, 20 minutes
Graphics/Displays:
Apple M1:
Chipset Model: Apple M1
Type: GPU
Bus: Built-In
Total Number of Cores: 8
Vendor: Apple (0x106b)
Metal Support: Metal 3
Displays:
D3218HN:
Resolution: 1920 x 1080 (1080p FHD - Full High Definition)
UI Looks like: 1920 x 1080 @ 60.00Hz
Main Display: Yes
Mirror: Off
Online: Yes
Rotation: Supported
Color LCD:
Display Type: Built-In Retina LCD
Resolution: 2560 x 1600 Retina
Mirror: Off
Online: Yes
Automatically Adjust Brightness: No
Connection Type: Internal
Installed Packages:
alabaster: 0.7.13
appdirs: 1.4.4
appnope: 0.1.3
asttokens: 2.2.1
Babel: 2.12.1
backcall: 0.2.0
beautifulsoup4: 4.11.2
blockdiag: 3.0.0
build: 0.10.0
certifi: 2021.10.8
cftime: 1.6.2
charset-normalizer: 3.1.0
ChimeraX-AddCharge: 1.5.9.1
ChimeraX-AddH: 2.2.5
ChimeraX-AlignmentAlgorithms: 2.0.1
ChimeraX-AlignmentHdrs: 3.3.1
ChimeraX-AlignmentMatrices: 2.1
ChimeraX-Alignments: 2.9.3
ChimeraX-AlphaFold: 1.0
ChimeraX-AltlocExplorer: 1.0.3
ChimeraX-AmberInfo: 1.0
ChimeraX-Arrays: 1.1
ChimeraX-Atomic: 1.43.10
ChimeraX-AtomicLibrary: 10.0.6
ChimeraX-AtomSearch: 2.0.1
ChimeraX-AxesPlanes: 2.3.2
ChimeraX-BasicActions: 1.1.2
ChimeraX-BILD: 1.0
ChimeraX-BlastProtein: 2.1.2
ChimeraX-BondRot: 2.0.1
ChimeraX-BugReporter: 1.0.1
ChimeraX-BuildStructure: 2.8
ChimeraX-Bumps: 1.0
ChimeraX-BundleBuilder: 1.2.2
ChimeraX-ButtonPanel: 1.0.1
ChimeraX-CageBuilder: 1.0.1
ChimeraX-CellPack: 1.0
ChimeraX-Centroids: 1.3.2
ChimeraX-ChangeChains: 1.0.2
ChimeraX-CheckWaters: 1.3.1
ChimeraX-ChemGroup: 2.0.1
ChimeraX-Clashes: 2.2.4
ChimeraX-Clipper: 0.21.0
ChimeraX-ColorActions: 1.0.3
ChimeraX-ColorGlobe: 1.0
ChimeraX-ColorKey: 1.5.3
ChimeraX-CommandLine: 1.2.5
ChimeraX-ConnectStructure: 2.0.1
ChimeraX-Contacts: 1.0.1
ChimeraX-Core: 1.6.1
ChimeraX-CoreFormats: 1.1
ChimeraX-coulombic: 1.4.2
ChimeraX-Crosslinks: 1.0
ChimeraX-Crystal: 1.0
ChimeraX-CrystalContacts: 1.0.1
ChimeraX-DataFormats: 1.2.3
ChimeraX-Dicom: 1.2
ChimeraX-DistMonitor: 1.4
ChimeraX-DockPrep: 1.1.1
ChimeraX-Dssp: 2.0
ChimeraX-EMDB-SFF: 1.0
ChimeraX-ESMFold: 1.0
ChimeraX-FileHistory: 1.0.1
ChimeraX-FunctionKey: 1.0.1
ChimeraX-Geometry: 1.3
ChimeraX-gltf: 1.0
ChimeraX-Graphics: 1.1.1
ChimeraX-Hbonds: 2.4
ChimeraX-Help: 1.2.1
ChimeraX-HKCage: 1.3
ChimeraX-IHM: 1.1
ChimeraX-ImageFormats: 1.2
ChimeraX-IMOD: 1.0
ChimeraX-IO: 1.0.1
ChimeraX-ISOLDE: 1.6.0
ChimeraX-ItemsInspection: 1.0.1
ChimeraX-Label: 1.1.7
ChimeraX-ListInfo: 1.1.1
ChimeraX-Log: 1.1.5
ChimeraX-LookingGlass: 1.1
ChimeraX-Maestro: 1.8.2
ChimeraX-Map: 1.1.4
ChimeraX-MapData: 2.0
ChimeraX-MapEraser: 1.0.1
ChimeraX-MapFilter: 2.0.1
ChimeraX-MapFit: 2.0
ChimeraX-MapSeries: 2.1.1
ChimeraX-Markers: 1.0.1
ChimeraX-Mask: 1.0.2
ChimeraX-MatchMaker: 2.0.12
ChimeraX-MDcrds: 2.6
ChimeraX-MedicalToolbar: 1.0.2
ChimeraX-Meeting: 1.0.1
ChimeraX-MLP: 1.1.1
ChimeraX-mmCIF: 2.12
ChimeraX-MMTF: 2.2
ChimeraX-Modeller: 1.5.9
ChimeraX-ModelPanel: 1.3.7
ChimeraX-ModelSeries: 1.0.1
ChimeraX-Mol2: 2.0
ChimeraX-Mole: 1.0
ChimeraX-Morph: 1.0.2
ChimeraX-MouseModes: 1.2
ChimeraX-Movie: 1.0
ChimeraX-Neuron: 1.0
ChimeraX-Nifti: 1.0
ChimeraX-NRRD: 1.0
ChimeraX-Nucleotides: 2.0.3
ChimeraX-OpenCommand: 1.10.1
ChimeraX-PDB: 2.7.2
ChimeraX-PDBBio: 1.0
ChimeraX-PDBLibrary: 1.0.2
ChimeraX-PDBMatrices: 1.0
ChimeraX-PhenixUI: 1.1.11
ChimeraX-PickBlobs: 1.0.1
ChimeraX-Positions: 1.0
ChimeraX-PresetMgr: 1.1
ChimeraX-PubChem: 2.1
ChimeraX-ReadPbonds: 1.0.1
ChimeraX-Registration: 1.1.1
ChimeraX-RemoteControl: 1.0
ChimeraX-RenderByAttr: 1.1
ChimeraX-RenumberResidues: 1.1
ChimeraX-ResidueFit: 1.0.1
ChimeraX-RestServer: 1.1
ChimeraX-RNALayout: 1.0
ChimeraX-RotamerLibMgr: 3.0
ChimeraX-RotamerLibsDunbrack: 2.0
ChimeraX-RotamerLibsDynameomics: 2.0
ChimeraX-RotamerLibsRichardson: 2.0
ChimeraX-SaveCommand: 1.5.1
ChimeraX-SchemeMgr: 1.0
ChimeraX-SDF: 2.0.1
ChimeraX-Segger: 1.0
ChimeraX-Segment: 1.0.1
ChimeraX-SelInspector: 1.0
ChimeraX-SeqView: 2.8.3
ChimeraX-Shape: 1.0.1
ChimeraX-Shell: 1.0.1
ChimeraX-Shortcuts: 1.1.1
ChimeraX-ShowSequences: 1.0.1
ChimeraX-SideView: 1.0.1
ChimeraX-Smiles: 2.1
ChimeraX-SmoothLines: 1.0
ChimeraX-SpaceNavigator: 1.0
ChimeraX-StdCommands: 1.10.3
ChimeraX-STL: 1.0.1
ChimeraX-Storm: 1.0
ChimeraX-StructMeasure: 1.1.2
ChimeraX-Struts: 1.0.1
ChimeraX-Surface: 1.0.1
ChimeraX-SwapAA: 2.0.1
ChimeraX-SwapRes: 2.2.1
ChimeraX-TapeMeasure: 1.0
ChimeraX-Test: 1.0
ChimeraX-Toolbar: 1.1.2
ChimeraX-ToolshedUtils: 1.2.1
ChimeraX-Topography: 1.0
ChimeraX-Tug: 1.0.1
ChimeraX-UI: 1.28.4
ChimeraX-uniprot: 2.2.2
ChimeraX-UnitCell: 1.0.1
ChimeraX-ViewDockX: 1.2
ChimeraX-VIPERdb: 1.0
ChimeraX-Vive: 1.1
ChimeraX-VolumeMenu: 1.0.1
ChimeraX-VTK: 1.0
ChimeraX-WavefrontOBJ: 1.0
ChimeraX-WebCam: 1.0.2
ChimeraX-WebServices: 1.1.1
ChimeraX-Zone: 1.0.1
colorama: 0.4.6
comm: 0.1.3
contourpy: 1.0.7
cxservices: 1.2.2
cycler: 0.11.0
Cython: 0.29.33
debugpy: 1.6.7
decorator: 5.1.1
docutils: 0.19
executing: 1.2.0
filelock: 3.9.0
fonttools: 4.39.3
funcparserlib: 1.0.1
grako: 3.16.5
h5py: 3.8.0
html2text: 2020.1.16
idna: 3.4
ihm: 0.35
imagecodecs: 2022.2.22
imagesize: 1.4.1
importlib-metadata: 6.6.0
ipykernel: 6.21.1
ipython: 8.10.0
ipython-genutils: 0.2.0
ipywidgets: 8.0.6
jedi: 0.18.2
Jinja2: 3.1.2
jupyter-client: 8.0.2
jupyter-core: 5.3.0
jupyterlab-widgets: 3.0.7
kiwisolver: 1.4.4
line-profiler: 4.0.2
lxml: 4.9.2
lz4: 4.3.2
MarkupSafe: 2.1.2
matplotlib: 3.6.3
matplotlib-inline: 0.1.6
msgpack: 1.0.4
nest-asyncio: 1.5.6
netCDF4: 1.6.2
networkx: 2.8.8
nibabel: 5.0.1
nptyping: 2.5.0
numexpr: 2.8.4
numpy: 1.23.5
openvr: 1.23.701
packaging: 21.3
ParmEd: 3.4.3
parso: 0.8.3
pep517: 0.13.0
pexpect: 4.8.0
pickleshare: 0.7.5
Pillow: 9.3.0
pip: 23.0
pkginfo: 1.9.6
platformdirs: 3.5.0
prompt-toolkit: 3.0.38
psutil: 5.9.4
ptyprocess: 0.7.0
pure-eval: 0.2.2
pycollada: 0.7.2
pydicom: 2.3.0
Pygments: 2.14.0
pynrrd: 1.0.0
PyOpenGL: 3.1.5
PyOpenGL-accelerate: 3.1.5
pyparsing: 3.0.9
pyproject-hooks: 1.0.0
PyQt6-commercial: 6.4.2
PyQt6-Qt6: 6.4.3
PyQt6-sip: 13.4.1
PyQt6-WebEngine-commercial: 6.4.0
PyQt6-WebEngine-Qt6: 6.4.3
python-dateutil: 2.8.2
pytz: 2023.3
pyzmq: 25.0.2
qtconsole: 5.4.0
QtPy: 2.3.1
RandomWords: 0.4.0
requests: 2.28.2
scipy: 1.9.3
setuptools: 67.4.0
setuptools-scm: 7.0.5
sfftk-rw: 0.7.3
six: 1.16.0
snowballstemmer: 2.2.0
sortedcontainers: 2.4.0
soupsieve: 2.4.1
sphinx: 6.1.3
sphinx-autodoc-typehints: 1.22
sphinxcontrib-applehelp: 1.0.4
sphinxcontrib-blockdiag: 3.0.0
sphinxcontrib-devhelp: 1.0.2
sphinxcontrib-htmlhelp: 2.0.1
sphinxcontrib-jsmath: 1.0.1
sphinxcontrib-qthelp: 1.0.3
sphinxcontrib-serializinghtml: 1.1.5
stack-data: 0.6.2
tables: 3.7.0
tcia-utils: 1.2.0
tifffile: 2022.10.10
tinyarray: 1.2.4
tomli: 2.0.1
tornado: 6.3.1
traitlets: 5.9.0
typing-extensions: 4.5.0
tzdata: 2023.3
urllib3: 1.26.15
wcwidth: 0.2.6
webcolors: 1.12
wheel: 0.38.4
wheel-filename: 1.4.1
widgetsnbextension: 4.0.7
zipp: 3.15.0
Hi Krishna,
--Eric