[Chimera-users] generate helical symmetry mates around an axis

[sadeem ahmad]

Hi all,
I am trying to generate a helical trimer from the monomer pdb around a
central double-stranded RNA. The rise and twist are 46 A and 74.0 deg
respectively. I opened my monomer pdb in chimera *(Screenshot-0)* and used
the following command to generate an axis around the central RNA double
helix:

*define axis #0:.X,.Y*

where X and Y are the chain IDs for the 2 RNA strands

This command helped me generate an axis named *a1* along the RNA duplex
*(Screenshot-1)*. Now I want to generate a helical trimer along the RNA
duplex with rise=46A and twist=74deg. For this I used the command:

*sym #0 group H,46,74,3 axis a1*

However, it is not placing the symmetric copies around axis a1 but around a
distant axis *(Screenshot-2)*. I am not sure how to generate the helical
trimer along the a1 axis. Any help in this regard would be appreciated.
Thanks.

-Sadeem


-- 
:-)
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