![[ChimeraX Issue Tracking System]](/trac/ChimeraX/chrome/site/ChimeraX-icon.svg){kind=icon}
Opened 5 days ago
Last modified 4 days ago
#20291 closed defect
Crash moving tool window, Mac multiple displays — at Version 1
| Reported by: | Owned by: | Tom Goddard | |
|---|---|---|---|
| Priority: | normal | Milestone: | |
| Component: | Window Toolkit | Version: | |
| Keywords: | Cc: | ||
| Blocked By: | Blocking: | ||
| Notify when closed: | Platform: | all | |
| Project: | ChimeraX |
Description (last modified by )
The following bug report has been submitted:
Platform: macOS-15.3.1-arm64-arm-64bit
ChimeraX Version: 1.11 (2025-12-17 06:45:39 UTC)
Description
Last time you used ChimeraX it crashed.
Please describe steps that led to the crash here.
Fatal Python error: Segmentation fault
Current thread 0x00000001ffe04840 (most recent call first):
File "/Applications/ChimeraX-1.11.app/Contents/Library/Frameworks/Python.framework/Versions/3.11/lib/python3.11/site-packages/chimerax/ui/gui.py", line 414 in event_loop
File "/Applications/ChimeraX-1.11.app/Contents/Library/Frameworks/Python.framework/Versions/3.11/lib/python3.11/site-packages/chimerax/core/__main__.py", line 1064 in init
File "/Applications/ChimeraX-1.11.app/Contents/Library/Frameworks/Python.framework/Versions/3.11/lib/python3.11/site-packages/chimerax/core/__main__.py", line 1229 in
File "", line 88 in _run_code
File "", line 198 in _run_module_as_main
Extension modules: chimerax.arrays._arrays, numpy.core._multiarray_umath, numpy.core._multiarray_tests, numpy.linalg._umath_linalg, numpy.fft._pocketfft_internal, numpy.random._common, numpy.random.bit_generator, numpy.random._bounded_integers, numpy.random._mt19937, numpy.random.mtrand, numpy.random._philox, numpy.random._pcg64, numpy.random._sfc64, numpy.random._generator, chimerax.geometry._geometry, PyQt6.QtCore, PyQt6.QtGui, PyQt6.QtWidgets, PyQt6.QtNetwork, PyQt6.QtPrintSupport, PyQt6.QtWebChannel, PyQt6.QtWebEngineCore, PyQt6.QtWebEngineWidgets, chimerax.atomic_lib._load_libs, tinyarray, chimerax.atomic.cymol, chimerax.atomic.cytmpl, chimerax.pdb_lib._load_libs, chimerax.surface._surface, chimerax.map._map, PIL._imaging, psutil._psutil_osx, psutil._psutil_posix, OpenGL_accelerate.errorchecker, OpenGL_accelerate.wrapper, OpenGL_accelerate.formathandler, OpenGL_accelerate.arraydatatype, OpenGL_accelerate.latebind, OpenGL_accelerate.vbo, chimerax.core._mac_util, OpenGL_accelerate.numpy_formathandler, OpenGL_accelerate.nones_formathandler, lz4._version, lz4.frame._frame, msgpack._cmsgpack, chimerax.core._serialize, PyQt6.QtOpenGL, PyQt6.QtOpenGLWidgets, lxml._elementpath, lxml.etree, PIL._imagingmath (total: 51)
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"codeSigningTeamID" : "LWV8X224YF",
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"vmRegionInfo" : "0x8 is not in any region. Bytes before following region: 4303585272
REGION TYPE START - END [ VSIZE] PRT\/MAX SHRMOD REGION DETAIL
UNUSED SPACE AT START
--->
__TEXT 100838000-10083c000 [ 16K] r-x\/r-x SM=COW \/Applications\/ChimeraX-1.11.app\/Contents\/MacOS\/ChimeraX",
"exception" : {"codes":"0x0000000000000001, 0x0000000000000008","rawCodes":[1,8],"type":"EXC_BAD_ACCESS","signal":"SIGSEGV","subtype":"KERN_INVALID_ADDRESS at 0x0000000000000008"},
"termination" : {"flags":0,"code":11,"namespace":"SIGNAL","indicator":"Segmentation fault: 11","byProc":"ChimeraX","byPid":94597},
"vmregioninfo" : "0x8 is not in any region. Bytes before following region: 4303585272
REGION TYPE START - END [ VSIZE] PRT\/MAX SHRMOD REGION DETAIL
UNUSED SPACE AT START
--->
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"name" : "numpy_formathandler.cpython-311-darwin.so"
},
{
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"uuid" : "5bd21324-288d-31d8-a98b-f8c1776ff590",
"path" : "\/Applications\/ChimeraX-1.11.app\/Contents\/Library\/Frameworks\/Python.framework\/Versions\/3.11\/lib\/python3.11\/site-packages\/OpenGL_accelerate\/nones_formathandler.cpython-311-darwin.so",
"name" : "nones_formathandler.cpython-311-darwin.so"
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{
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"uuid" : "322fc538-890c-37d6-b941-662f1083ca42",
"path" : "\/Applications\/ChimeraX-1.11.app\/Contents\/Library\/Frameworks\/Python.framework\/Versions\/3.11\/lib\/python3.11\/site-packages\/lz4\/_version.cpython-311-darwin.so",
"name" : "_version.cpython-311-darwin.so"
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{
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"uuid" : "83f39565-33c4-320a-ac75-81b90af76e46",
"path" : "\/Applications\/ChimeraX-1.11.app\/Contents\/Library\/Frameworks\/Python.framework\/Versions\/3.11\/lib\/python3.11\/site-packages\/lz4\/frame\/_frame.cpython-311-darwin.so",
"name" : "_frame.cpython-311-darwin.so"
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{
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"uuid" : "bef79d77-164b-32c8-b975-670434b8d743",
"path" : "\/Applications\/ChimeraX-1.11.app\/Contents\/Library\/Frameworks\/Python.framework\/Versions\/3.11\/lib\/python3.11\/site-packages\/chimerax\/core\/_serialize.cpython-311-darwin.so",
"name" : "_serialize.cpython-311-darwin.so"
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{
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"uuid" : "4054e3d0-2474-37de-901e-4c10afdac686",
"path" : "\/Applications\/ChimeraX-1.11.app\/Contents\/Library\/Frameworks\/Python.framework\/Versions\/3.11\/lib\/python3.11\/site-packages\/msgpack\/_cmsgpack.cpython-311-darwin.so",
"name" : "_cmsgpack.cpython-311-darwin.so"
},
{
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"uuid" : "f660af98-8bcc-35ff-a0a7-91590a2716eb",
"path" : "\/Applications\/ChimeraX-1.11.app\/Contents\/Library\/Frameworks\/Python.framework\/Versions\/3.11\/lib\/python3.11\/lib-dynload\/_csv.cpython-311-darwin.so",
"name" : "_csv.cpython-311-darwin.so"
},
{
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"uuid" : "0d5e2b04-5266-3fcf-8917-51cab65a30b4",
"path" : "\/Applications\/ChimeraX-1.11.app\/Contents\/Library\/Frameworks\/Python.framework\/Versions\/3.11\/lib\/python3.11\/site-packages\/PyQt6\/QtOpenGL.abi3.so",
"name" : "QtOpenGL.abi3.so"
},
{
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"uuid" : "6159a840-3c3a-3d9e-9af1-35dea6d209e4",
"path" : "\/Applications\/ChimeraX-1.11.app\/Contents\/Library\/Frameworks\/Python.framework\/Versions\/3.11\/lib\/python3.11\/site-packages\/PyQt6\/QtOpenGLWidgets.abi3.so",
"name" : "QtOpenGLWidgets.abi3.so"
},
{
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"uuid" : "bdbfbc59-c41d-3a69-8112-739716f2e836",
"path" : "\/Applications\/ChimeraX-1.11.app\/Contents\/Library\/Frameworks\/Python.framework\/Versions\/3.11\/lib\/python3.11\/site-packages\/PyQt6\/Qt6\/lib\/QtOpenGLWidgets.framework\/Versions\/A\/QtOpenGLWidgets",
"name" : "QtOpenGLWidgets"
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{
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"uuid" : "06082bf4-58c8-3f16-9f71-841cf9880fbe",
"path" : "\/Applications\/ChimeraX-1.11.app\/Contents\/Library\/Frameworks\/Python.framework\/Versions\/3.11\/lib\/python3.11\/site-packages\/lxml\/etree.cpython-311-darwin.so",
"name" : "etree.cpython-311-darwin.so"
},
{
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"uuid" : "63b5bd02-9626-3588-a39c-88d4f882312b",
"path" : "\/Applications\/ChimeraX-1.11.app\/Contents\/Library\/Frameworks\/Python.framework\/Versions\/3.11\/lib\/python3.11\/site-packages\/lxml\/_elementpath.cpython-311-darwin.so",
"name" : "_elementpath.cpython-311-darwin.so"
},
{
"source" : "P",
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"base" : 14006747136,
"size" : 32768,
"uuid" : "7c43df7b-5e3e-3a91-9bc6-b8f9ebd02ec2",
"path" : "\/Applications\/ChimeraX-1.11.app\/Contents\/Library\/Frameworks\/Python.framework\/Versions\/3.11\/lib\/python3.11\/site-packages\/PIL\/_imagingmath.cpython-311-darwin.so",
"name" : "_imagingmath.cpython-311-darwin.so"
},
{
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"path" : "\/usr\/lib\/system\/libsystem_kernel.dylib",
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{
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"uuid" : "642faf7a-874e-37e6-8aba-2b0cc09a3025",
"path" : "\/usr\/lib\/system\/libsystem_pthread.dylib",
"name" : "libsystem_pthread.dylib"
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{
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"uuid" : "92699527-645f-3d8d-aed8-1cfb0c034e15",
"path" : "\/usr\/lib\/system\/libsystem_c.dylib",
"name" : "libsystem_c.dylib"
},
{
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"base" : 6816559104,
"size" : 32748,
"uuid" : "984d08b8-f85c-36f5-9e17-5bff7fc779a3",
"path" : "\/usr\/lib\/system\/libsystem_platform.dylib",
"name" : "libsystem_platform.dylib"
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{
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"name" : "CoreFoundation",
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{
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"size" : 14974976,
"uuid" : "16d282d0-8b48-3e76-8036-fcb45dece518",
"path" : "\/System\/Library\/Frameworks\/Foundation.framework\/Versions\/C\/Foundation",
"name" : "Foundation",
"CFBundleVersion" : "3302.1.400"
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{
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{
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REGION TYPE SIZE COUNT (non-coalesced)
=========== ======= =======
Accelerate framework 256K 2
Activity Tracing 256K 1
CG image 384K 20
ColorSync 640K 32
CoreAnimation 1776K 111
CoreGraphics 64K 4
CoreGraphics (reserved) 16K 1 reserved VM address space (unallocated)
CoreUI image data 5232K 48
Foundation 48K 2
Kernel Alloc Once 32K 1
MALLOC 4.6G 137
MALLOC guard page 288K 18
Mach message 64K 3
OpenGL GLSL 256K 3
PROTECTED_MEMORY 16K 1
STACK GUARD 768K 48
Stack 198.1M 49
Stack Guard 56.0M 1
VM_ALLOCATE 302.5M 125
VM_ALLOCATE (reserved) 288.0M 3 reserved VM address space (unallocated)
__AUTH 5284K 672
__AUTH_CONST 70.5M 920
__CTF 824 1
__DATA 32.5M 1037
__DATA_CONST 50.5M 1056
__DATA_DIRTY 2763K 342
__FONT_DATA 2352 1
__GLSLBUILTINS 5174K 1
__INFO_FILTER 8 1
__LINKEDIT 637.0M 130
__OBJC_RW 2374K 1
__TEXT 1.3G 1076
__TPRO_CONST 272K 2
dyld private memory 48K 2
mapped file 296.6M 59
owned unmapped memory 87.2M 1
page table in kernel 2868K 1
shared memory 3152K 22
=========== ======= =======
TOTAL 7.9G 5935
TOTAL, minus reserved VM space 7.6G 5935
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},
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===== Log before crash start =====
UCSF ChimeraX version: 1.11 (2025-12-17)
© 2016-2025 Regents of the University of California. All rights reserved.
> open "/Users/peiyuyu/Documents/Goethe
> University/2026/5_Manuscripts/1_AcrF/Figure/Figure_cryoEM_workflow/SA-
> F_B1/20260507_SA-F_B1_workflow5.cxs"
Opened cryosparc_P4_J216_class_03_00122_volume_sharp.mrc as #4, grid size
128,128,128, pixel 2.08, shown at level 0.203, step 1, values float32
Opened cryosparc_P4_J216_class_04_00122_volume_sharp.mrc as #5, grid size
128,128,128, pixel 2.08, shown at level 0.206, step 1, values float32
Opened CS_P4_J216_class_0_sharp_flip.mrc as #1, grid size 128,128,128, pixel
2.08, shown at level 0.185, step 1, values float32
Opened CS_P4_J216_class_1_sharp_flip.mrc as #2, grid size 128,128,128, pixel
2.08, shown at level 0.203, step 1, values float32
Opened CS_P4_J216_class_2_sharp_flip.mrc as #3, grid size 128,128,128, pixel
2.08, shown at level 0.356, step 1, values float32
Opened CS_P4_J219_sharp_flip.mrc as #6, grid size 80,80,80, pixel 3.32, shown
at level 1.17, step 1, values float32
Opened CS_P4_J244_sharp_flip.mrc as #7, grid size 80,80,80, pixel 3.32, shown
at level 1.16, step 1, values float32
Opened cryosparc_P4_J242_class_00_00122_volume_sharp.mrc as #8, grid size
128,128,128, pixel 2.08, shown at level 0.221, step 1, values float32
Opened cryosparc_P4_J242_class_01_00122_volume_sharp.mrc as #9, grid size
128,128,128, pixel 2.08, shown at level 0.146, step 1, values float32
Opened cryosparc_P4_J242_class_02_00122_volume_sharp.mrc as #10, grid size
128,128,128, pixel 2.08, shown at level 0.195, step 1, values float32
Opened cryosparc_P4_J242_class_03_00122_volume_sharp.mrc as #11, grid size
128,128,128, pixel 2.08, shown at level 0.15, step 1, values float32
Opened cryosparc_P4_J242_class_04_00122_volume_sharp.mrc as #12, grid size
128,128,128, pixel 2.08, shown at level 0.345, step 1, values float32
Opened cryosparc_P4_J117_class_00_00162_volume_sharp.mrc as #13, grid size
128,128,128, pixel 2.08, shown at level 0.163, step 1, values float32
Opened cryosparc_P4_J117_class_01_00162_volume_sharp.mrc as #14, grid size
128,128,128, pixel 2.08, shown at level 0.187, step 1, values float32
Opened CS_P4_J117_class_02_sharp_flip.mrc as #15, grid size 128,128,128, pixel
2.08, shown at level 0.335, step 1, values float32
Opened cryosparc_P4_J117_class_03_00162_volume_sharp.mrc as #16, grid size
128,128,128, pixel 2.08, shown at level 0.179, step 1, values float32
Opened cryosparc_P4_J117_class_04_00162_volume_sharp.mrc as #17, grid size
128,128,128, pixel 2.08, shown at level 0.206, step 1, values float32
Opened CS_P4_J120_sharp_flip.mrc as #18, grid size 80,80,80, pixel 3.32, shown
at level 1.29, step 1, values float32
Opened cryosparc_P4_J169_class_00_00182_volume_sharp.mrc as #19, grid size
128,128,128, pixel 2.08, shown at level 0.156, step 1, values float32
Opened cryosparc_P4_J169_class_01_00182_volume_sharp.mrc as #20, grid size
128,128,128, pixel 2.08, shown at level 0.198, step 1, values float32
Opened cryosparc_P4_J169_class_02_00182_volume_sharp.mrc as #21, grid size
128,128,128, pixel 2.08, shown at level 0.248, step 1, values float32
Opened CS_P4_J169_class_03_sharp_flip.mrc as #22, grid size 128,128,128, pixel
2.08, shown at level 0.271, step 1, values float32
Opened cryosparc_P4_J169_class_04_00182_volume_sharp.mrc as #23, grid size
128,128,128, pixel 2.08, shown at level 0.2, step 1, values float32
Opened CS_P4_J176_NU_sharp_flip.mrc as #24, grid size 80,80,80, pixel 3.32,
shown at level 1.6, step 1, values float32
Opened CS_P4_J255_sharp_flip.mrc as #25, grid size 80,80,80, pixel 3.32, shown
at level 0.649, step 1, values float32
Opened CS_P4_J256_sharp_flip.mrc as #26, grid size 80,80,80, pixel 3.32, shown
at level 1.01, step 1, values float32
Opened CS_P4_J257_sharp_flip.mrc as #27, grid size 80,80,80, pixel 3.32, shown
at level 1.16, step 1, values float32
Opened CS_P4_J258_sharp_flip.mrc as #28, grid size 80,80,80, pixel 3.32, shown
at level 0.533, step 1, values float32
Opened CS_P4_J259_sharp_flip.mrc as #29, grid size 80,80,80, pixel 3.32, shown
at level 0.567, step 1, values float32
Opened CS_P4_J267_sharp_flip.mrc as #30, grid size 320,320,320, pixel 0.831,
shown at level 0.134, step 1, values float32
Opened CS_P4_J277_sharp_flip.mrc as #31, grid size 320,320,320, pixel 0.831,
shown at level 0.143, step 1, values float32
Opened cryosparc_P4_J282_005_volume_map_sharp.mrc as #32, grid size
320,320,320, pixel 0.831, shown at level 0.0548, step 2, values float32
Opened cryosparc_P4_J285_006_volume_map_sharp.mrc as #33, grid size
320,320,320, pixel 0.831, shown at level 0.0508, step 2, values float32
Opened cryosparc_P4_J287_map_locres.mrc as #34, grid size 320,320,320, pixel
0.831, shown at level 3.78, step 2, values float32
Opened cryosparc_P4_J288_map_locres.mrc as #35, grid size 320,320,320, pixel
0.831, shown at level 3.77, step 2, values float32
Opened cryosparc_P4_J282_005_volume_map_sharp.mrc z flip as #37, grid size
320,320,320, pixel 0.831, shown at level 0.065, step 1, values float32
Opened cryosparc_P4_J287_map_locres.mrc z flip as #38, grid size 320,320,320,
pixel 0.831, shown at level 3.78, step 1, values float32
Opened cryosparc_P4_J285_006_volume_map_sharp.mrc z flip as #39, grid size
320,320,320, pixel 0.831, shown at level 0.0664, step 1, values float32
Opened cryosparc_P4_J288_map_locres.mrc z flip as #40, grid size 320,320,320,
pixel 0.831, shown at level 3.77, step 1, values float32
Opened cryosparc_P4_J242_class_01_00122_volume_sharp.mrc as #42, grid size
128,128,128, pixel 2.08, shown at level 0.254, step 1, values float32
Opened CS_P4_J282_sharp_flip.mrc as #36, grid size 320,320,320, pixel 0.831,
shown at level 0.0602, step 2, values float32
Opened cryosparc_P4_J169_class_02_00182_volume_sharp.mrc as #43, grid size
128,128,128, pixel 2.08, shown at level 0.228, step 1, values float32
Opened CS_P4_J295_sharp_flip.mrc as #44, grid size 320,320,320, pixel 0.831,
shown at level 0.0515, step 1, values float32
Opened CS_P4_J296_locres_flip.mrc as #45, grid size 320,320,320, pixel 0.831,
shown at level 8.66, step 1, values float32
Opened CS_P4_J300_sharp_flip.mrc as #46, grid size 320,320,320, pixel 0.831,
shown at level 0.0592, step 1, values float32
Opened CS_P4_J301_locres_flip.mrc as #47, grid size 320,320,320, pixel 0.831,
shown at level 9.68, step 1, values float32
Opened CS_P4_J302_sharp_flip.mrc as #48, grid size 80,80,80, pixel 3.32, shown
at level 0.612, step 1, values float32
Opened CS_P4_J303_sharp_flip.mrc as #49, grid size 80,80,80, pixel 3.32, shown
at level 0.575, step 1, values float32
Opened CS_P4_J304_sharp_flip.mrc as #50, grid size 80,80,80, pixel 3.32, shown
at level 1.95, step 1, values float32
Opened CS_P4_J305_sharp_flip.mrc as #51, grid size 80,80,80, pixel 3.32, shown
at level 0.569, step 1, values float32
Opened CS_P4_J306_sharp_flip.mrc as #52, grid size 80,80,80, pixel 3.32, shown
at level 1.03, step 1, values float32
Opened CS_P4_J307_sharp_flip.mrc as #53, grid size 80,80,80, pixel 3.32, shown
at level 0.417, step 1, values float32
Opened CS_P4_J308_sharp_flip.mrc as #54, grid size 80,80,80, pixel 3.32, shown
at level 0.487, step 1, values float32
Opened CS_P4_J309_sharp_flip.mrc as #55, grid size 80,80,80, pixel 3.32, shown
at level 0.427, step 1, values float32
Opened CS_P4_J310_sharp_flip.mrc as #56, grid size 80,80,80, pixel 3.32, shown
at level 0.719, step 1, values float32
Opened CS_P4_J311_sharp_flip.mrc as #57, grid size 80,80,80, pixel 3.32, shown
at level 0.525, step 1, values float32
Opened CS_P4_J323_sharp_flip.mrc as #58, grid size 320,320,320, pixel 0.831,
shown at level 0.0616, step 1, values float32
Opened CS_P4_J324_map_locres_flip.mrc as #59, grid size 320,320,320, pixel
0.831, shown at level 3.69, step 1, values float32
Opened CS_P4_J329_sharp_flip.mrc as #60, grid size 320,320,320, pixel 0.831,
shown at level 0.0566, step 1, values float32
Opened CS_P4_J330_locres_flip.mrc as #61, grid size 320,320,320, pixel 0.831,
shown at level 9.71, step 1, values float32
Opened CS_P4_J300_sharp_flip.mrc as #62, grid size 320,320,320, pixel 0.831,
shown at level 0.0575, step 1, values float32
Opened CS_P4_J301_locres_flip.mrc as #63, grid size 320,320,320, pixel 0.831,
shown at level 3.95, step 1, values float32
Log from Thu May 7 21:11:39 2026UCSF ChimeraX version: 1.11 (2025-12-17)
© 2016-2025 Regents of the University of California. All rights reserved.
> open "/Users/peiyuyu/Documents/Goethe
> University/2026/5_Manuscripts/1_AcrF/Figure/Figure_cryoEM_workflow/SA-
> F_B1/20260507_SA-F_B1_workflow3.cxs"
Opened cryosparc_P4_J216_class_03_00122_volume_sharp.mrc as #4, grid size
128,128,128, pixel 2.08, shown at level 0.203, step 1, values float32
Opened cryosparc_P4_J216_class_04_00122_volume_sharp.mrc as #5, grid size
128,128,128, pixel 2.08, shown at level 0.206, step 1, values float32
Opened CS_P4_J216_class_0_sharp_flip.mrc as #1, grid size 128,128,128, pixel
2.08, shown at level 0.185, step 1, values float32
Opened CS_P4_J216_class_1_sharp_flip.mrc as #2, grid size 128,128,128, pixel
2.08, shown at level 0.203, step 1, values float32
Opened CS_P4_J216_class_2_sharp_flip.mrc as #3, grid size 128,128,128, pixel
2.08, shown at level 0.356, step 1, values float32
Opened CS_P4_J219_sharp_flip.mrc as #6, grid size 80,80,80, pixel 3.32, shown
at level 1.17, step 1, values float32
Opened CS_P4_J244_sharp_flip.mrc as #7, grid size 80,80,80, pixel 3.32, shown
at level 1.16, step 1, values float32
Opened cryosparc_P4_J242_class_00_00122_volume_sharp.mrc as #8, grid size
128,128,128, pixel 2.08, shown at level 0.221, step 1, values float32
Opened cryosparc_P4_J242_class_01_00122_volume_sharp.mrc as #9, grid size
128,128,128, pixel 2.08, shown at level 0.146, step 1, values float32
Opened cryosparc_P4_J242_class_02_00122_volume_sharp.mrc as #10, grid size
128,128,128, pixel 2.08, shown at level 0.195, step 1, values float32
Opened cryosparc_P4_J242_class_03_00122_volume_sharp.mrc as #11, grid size
128,128,128, pixel 2.08, shown at level 0.15, step 1, values float32
Opened cryosparc_P4_J242_class_04_00122_volume_sharp.mrc as #12, grid size
128,128,128, pixel 2.08, shown at level 0.345, step 1, values float32
Opened cryosparc_P4_J117_class_00_00162_volume_sharp.mrc as #13, grid size
128,128,128, pixel 2.08, shown at level 0.163, step 1, values float32
Opened cryosparc_P4_J117_class_01_00162_volume_sharp.mrc as #14, grid size
128,128,128, pixel 2.08, shown at level 0.187, step 1, values float32
Opened CS_P4_J117_class_02_sharp_flip.mrc as #15, grid size 128,128,128, pixel
2.08, shown at level 0.335, step 1, values float32
Opened cryosparc_P4_J117_class_03_00162_volume_sharp.mrc as #16, grid size
128,128,128, pixel 2.08, shown at level 0.179, step 1, values float32
Opened cryosparc_P4_J117_class_04_00162_volume_sharp.mrc as #17, grid size
128,128,128, pixel 2.08, shown at level 0.206, step 1, values float32
Opened CS_P4_J120_sharp_flip.mrc as #18, grid size 80,80,80, pixel 3.32, shown
at level 1.29, step 1, values float32
Opened cryosparc_P4_J169_class_00_00182_volume_sharp.mrc as #19, grid size
128,128,128, pixel 2.08, shown at level 0.156, step 1, values float32
Opened cryosparc_P4_J169_class_01_00182_volume_sharp.mrc as #20, grid size
128,128,128, pixel 2.08, shown at level 0.198, step 1, values float32
Opened cryosparc_P4_J169_class_02_00182_volume_sharp.mrc as #21, grid size
128,128,128, pixel 2.08, shown at level 0.248, step 1, values float32
Opened CS_P4_J169_class_03_sharp_flip.mrc as #22, grid size 128,128,128, pixel
2.08, shown at level 0.271, step 1, values float32
Opened cryosparc_P4_J169_class_04_00182_volume_sharp.mrc as #23, grid size
128,128,128, pixel 2.08, shown at level 0.2, step 1, values float32
Opened CS_P4_J176_NU_sharp_flip.mrc as #24, grid size 80,80,80, pixel 3.32,
shown at level 1.6, step 1, values float32
Opened CS_P4_J255_sharp_flip.mrc as #25, grid size 80,80,80, pixel 3.32, shown
at level 0.649, step 1, values float32
Opened CS_P4_J256_sharp_flip.mrc as #26, grid size 80,80,80, pixel 3.32, shown
at level 1.01, step 1, values float32
Opened CS_P4_J257_sharp_flip.mrc as #27, grid size 80,80,80, pixel 3.32, shown
at level 1.16, step 1, values float32
Opened CS_P4_J258_sharp_flip.mrc as #28, grid size 80,80,80, pixel 3.32, shown
at level 0.533, step 1, values float32
Opened CS_P4_J259_sharp_flip.mrc as #29, grid size 80,80,80, pixel 3.32, shown
at level 0.567, step 1, values float32
Opened CS_P4_J267_sharp_flip.mrc as #30, grid size 320,320,320, pixel 0.831,
shown at level 0.134, step 1, values float32
Opened CS_P4_J277_sharp_flip.mrc as #31, grid size 320,320,320, pixel 0.831,
shown at level 0.143, step 1, values float32
Opened cryosparc_P4_J282_005_volume_map_sharp.mrc as #32, grid size
320,320,320, pixel 0.831, shown at level 0.0548, step 2, values float32
Opened cryosparc_P4_J285_006_volume_map_sharp.mrc as #33, grid size
320,320,320, pixel 0.831, shown at level 0.0508, step 2, values float32
Opened cryosparc_P4_J287_map_locres.mrc as #34, grid size 320,320,320, pixel
0.831, shown at level 3.78, step 2, values float32
Opened cryosparc_P4_J288_map_locres.mrc as #35, grid size 320,320,320, pixel
0.831, shown at level 3.77, step 2, values float32
Opened cryosparc_P4_J282_005_volume_map_sharp.mrc z flip as #37, grid size
320,320,320, pixel 0.831, shown at level 0.065, step 1, values float32
Opened cryosparc_P4_J287_map_locres.mrc z flip as #38, grid size 320,320,320,
pixel 0.831, shown at level 3.78, step 1, values float32
Opened cryosparc_P4_J285_006_volume_map_sharp.mrc z flip as #39, grid size
320,320,320, pixel 0.831, shown at level 0.0664, step 1, values float32
Opened cryosparc_P4_J288_map_locres.mrc z flip as #40, grid size 320,320,320,
pixel 0.831, shown at level 3.77, step 1, values float32
Opened cryosparc_P4_J242_class_01_00122_volume_sharp.mrc as #42, grid size
128,128,128, pixel 2.08, shown at level 0.254, step 1, values float32
Opened CS_P4_J282_sharp_flip.mrc as #36, grid size 320,320,320, pixel 0.831,
shown at level 0.0602, step 2, values float32
Opened cryosparc_P4_J169_class_02_00182_volume_sharp.mrc as #43, grid size
128,128,128, pixel 2.08, shown at level 0.228, step 1, values float32
Opened CS_P4_J295_sharp_flip.mrc as #44, grid size 320,320,320, pixel 0.831,
shown at level 0.0515, step 1, values float32
Opened CS_P4_J296_locres_flip.mrc as #45, grid size 320,320,320, pixel 0.831,
shown at level 8.66, step 1, values float32
Opened CS_P4_J300_sharp_flip.mrc as #46, grid size 320,320,320, pixel 0.831,
shown at level 0.0592, step 1, values float32
Opened CS_P4_J301_locres_flip.mrc as #47, grid size 320,320,320, pixel 0.831,
shown at level 9.68, step 1, values float32
Opened CS_P4_J302_sharp_flip.mrc as #48, grid size 80,80,80, pixel 3.32, shown
at level 0.612, step 1, values float32
Opened CS_P4_J303_sharp_flip.mrc as #49, grid size 80,80,80, pixel 3.32, shown
at level 0.575, step 1, values float32
Opened CS_P4_J304_sharp_flip.mrc as #50, grid size 80,80,80, pixel 3.32, shown
at level 1.95, step 1, values float32
Opened CS_P4_J305_sharp_flip.mrc as #51, grid size 80,80,80, pixel 3.32, shown
at level 0.569, step 1, values float32
Opened CS_P4_J306_sharp_flip.mrc as #52, grid size 80,80,80, pixel 3.32, shown
at level 1.03, step 1, values float32
Opened CS_P4_J307_sharp_flip.mrc as #53, grid size 80,80,80, pixel 3.32, shown
at level 0.417, step 1, values float32
Opened CS_P4_J308_sharp_flip.mrc as #54, grid size 80,80,80, pixel 3.32, shown
at level 0.487, step 1, values float32
Opened CS_P4_J309_sharp_flip.mrc as #55, grid size 80,80,80, pixel 3.32, shown
at level 0.427, step 1, values float32
Opened CS_P4_J310_sharp_flip.mrc as #56, grid size 80,80,80, pixel 3.32, shown
at level 0.719, step 1, values float32
Opened CS_P4_J311_sharp_flip.mrc as #57, grid size 80,80,80, pixel 3.32, shown
at level 0.525, step 1, values float32
Opened CS_P4_J323_sharp_flip.mrc as #58, grid size 320,320,320, pixel 0.831,
shown at level 0.0616, step 1, values float32
Opened CS_P4_J324_map_locres_flip.mrc as #59, grid size 320,320,320, pixel
0.831, shown at level 3.69, step 1, values float32
Opened CS_P4_J329_sharp_flip.mrc as #60, grid size 320,320,320, pixel 0.831,
shown at level 0.0566, step 1, values float32
Opened CS_P4_J330_locres_flip.mrc as #61, grid size 320,320,320, pixel 0.831,
shown at level 9.71, step 1, values float32
Log from Thu May 7 15:58:17 2026UCSF ChimeraX version: 1.11 (2025-12-17)
© 2016-2025 Regents of the University of California. All rights reserved.
> open "/Users/peiyuyu/Documents/Goethe
> University/2026/5_Manuscripts/1_AcrF/Figure/Figure_cryoEM_workflow/SA-
> F_B1/20260506_SA-F_B1_workflow.cxs"
Opened cryosparc_P4_J216_class_03_00122_volume_sharp.mrc as #4, grid size
128,128,128, pixel 2.08, shown at level 0.203, step 1, values float32
Opened cryosparc_P4_J216_class_04_00122_volume_sharp.mrc as #5, grid size
128,128,128, pixel 2.08, shown at level 0.206, step 1, values float32
Opened CS_P4_J216_class_0_sharp_flip.mrc as #1, grid size 128,128,128, pixel
2.08, shown at level 0.185, step 1, values float32
Opened CS_P4_J216_class_1_sharp_flip.mrc as #2, grid size 128,128,128, pixel
2.08, shown at level 0.203, step 1, values float32
Opened CS_P4_J216_class_2_sharp_flip.mrc as #3, grid size 128,128,128, pixel
2.08, shown at level 0.356, step 1, values float32
Opened CS_P4_J219_sharp_flip.mrc as #6, grid size 80,80,80, pixel 3.32, shown
at level 1.17, step 1, values float32
Opened CS_P4_J244_sharp_flip.mrc as #7, grid size 80,80,80, pixel 3.32, shown
at level 1.16, step 1, values float32
Opened cryosparc_P4_J242_class_00_00122_volume_sharp.mrc as #8, grid size
128,128,128, pixel 2.08, shown at level 0.221, step 1, values float32
Opened cryosparc_P4_J242_class_01_00122_volume_sharp.mrc as #9, grid size
128,128,128, pixel 2.08, shown at level 0.146, step 1, values float32
Opened cryosparc_P4_J242_class_02_00122_volume_sharp.mrc as #10, grid size
128,128,128, pixel 2.08, shown at level 0.195, step 1, values float32
Opened cryosparc_P4_J242_class_03_00122_volume_sharp.mrc as #11, grid size
128,128,128, pixel 2.08, shown at level 0.15, step 1, values float32
Opened cryosparc_P4_J242_class_04_00122_volume_sharp.mrc as #12, grid size
128,128,128, pixel 2.08, shown at level 0.345, step 1, values float32
Opened cryosparc_P4_J117_class_00_00162_volume_sharp.mrc as #13, grid size
128,128,128, pixel 2.08, shown at level 0.163, step 1, values float32
Opened cryosparc_P4_J117_class_01_00162_volume_sharp.mrc as #14, grid size
128,128,128, pixel 2.08, shown at level 0.187, step 1, values float32
Opened CS_P4_J117_class_02_sharp_flip.mrc as #15, grid size 128,128,128, pixel
2.08, shown at level 0.335, step 1, values float32
Opened cryosparc_P4_J117_class_03_00162_volume_sharp.mrc as #16, grid size
128,128,128, pixel 2.08, shown at level 0.179, step 1, values float32
Opened cryosparc_P4_J117_class_04_00162_volume_sharp.mrc as #17, grid size
128,128,128, pixel 2.08, shown at level 0.206, step 1, values float32
Opened CS_P4_J120_sharp_flip.mrc as #18, grid size 80,80,80, pixel 3.32, shown
at level 1.29, step 1, values float32
Opened cryosparc_P4_J169_class_00_00182_volume_sharp.mrc as #19, grid size
128,128,128, pixel 2.08, shown at level 0.156, step 1, values float32
Opened cryosparc_P4_J169_class_01_00182_volume_sharp.mrc as #20, grid size
128,128,128, pixel 2.08, shown at level 0.198, step 1, values float32
Opened cryosparc_P4_J169_class_02_00182_volume_sharp.mrc as #21, grid size
128,128,128, pixel 2.08, shown at level 0.248, step 1, values float32
Opened CS_P4_J169_class_03_sharp_flip.mrc as #22, grid size 128,128,128, pixel
2.08, shown at level 0.271, step 1, values float32
Opened cryosparc_P4_J169_class_04_00182_volume_sharp.mrc as #23, grid size
128,128,128, pixel 2.08, shown at level 0.2, step 1, values float32
Opened CS_P4_J176_NU_sharp_flip.mrc as #24, grid size 80,80,80, pixel 3.32,
shown at level 1.6, step 1, values float32
Opened CS_P4_J255_sharp_flip.mrc as #25, grid size 80,80,80, pixel 3.32, shown
at level 0.649, step 1, values float32
Opened CS_P4_J256_sharp_flip.mrc as #26, grid size 80,80,80, pixel 3.32, shown
at level 1.01, step 1, values float32
Opened CS_P4_J257_sharp_flip.mrc as #27, grid size 80,80,80, pixel 3.32, shown
at level 1.16, step 1, values float32
Opened CS_P4_J258_sharp_flip.mrc as #28, grid size 80,80,80, pixel 3.32, shown
at level 0.533, step 1, values float32
Opened CS_P4_J259_sharp_flip.mrc as #29, grid size 80,80,80, pixel 3.32, shown
at level 0.567, step 1, values float32
Opened CS_P4_J267_sharp_flip.mrc as #30, grid size 320,320,320, pixel 0.831,
shown at level 0.134, step 1, values float32
Opened CS_P4_J277_sharp_flip.mrc as #31, grid size 320,320,320, pixel 0.831,
shown at level 0.143, step 1, values float32
Opened cryosparc_P4_J282_005_volume_map_sharp.mrc as #32, grid size
320,320,320, pixel 0.831, shown at level 0.0548, step 2, values float32
Opened cryosparc_P4_J285_006_volume_map_sharp.mrc as #33, grid size
320,320,320, pixel 0.831, shown at level 0.0508, step 2, values float32
Opened cryosparc_P4_J287_map_locres.mrc as #34, grid size 320,320,320, pixel
0.831, shown at level 3.78, step 2, values float32
Opened cryosparc_P4_J288_map_locres.mrc as #35, grid size 320,320,320, pixel
0.831, shown at level 3.77, step 2, values float32
Opened cryosparc_P4_J282_005_volume_map_sharp.mrc z flip as #37, grid size
320,320,320, pixel 0.831, shown at level 0.065, step 1, values float32
Opened cryosparc_P4_J287_map_locres.mrc z flip as #38, grid size 320,320,320,
pixel 0.831, shown at level 3.78, step 1, values float32
Opened cryosparc_P4_J285_006_volume_map_sharp.mrc z flip as #39, grid size
320,320,320, pixel 0.831, shown at level 0.0664, step 1, values float32
Opened cryosparc_P4_J288_map_locres.mrc z flip as #40, grid size 320,320,320,
pixel 0.831, shown at level 3.77, step 1, values float32
Opened cryosparc_P4_J242_class_01_00122_volume_sharp.mrc as #42, grid size
128,128,128, pixel 2.08, shown at level 0.254, step 1, values float32
Opened CS_P4_J282_sharp_flip.mrc as #36, grid size 320,320,320, pixel 0.831,
shown at level 0.0602, step 2, values float32
Opened cryosparc_P4_J169_class_02_00182_volume_sharp.mrc as #43, grid size
128,128,128, pixel 2.08, shown at level 0.228, step 1, values float32
Opened CS_P4_J295_sharp_flip.mrc as #44, grid size 320,320,320, pixel 0.831,
shown at level 0.0515, step 1, values float32
Opened CS_P4_J296_locres_flip.mrc as #45, grid size 320,320,320, pixel 0.831,
shown at level 8.66, step 1, values float32
Opened CS_P4_J300_sharp_flip.mrc as #46, grid size 320,320,320, pixel 0.831,
shown at level 0.0592, step 1, values float32
Opened CS_P4_J301_locres_flip.mrc as #47, grid size 320,320,320, pixel 0.831,
shown at level 9.68, step 1, values float32
Opened CS_P4_J302_sharp_flip.mrc as #48, grid size 80,80,80, pixel 3.32, shown
at level 0.612, step 1, values float32
Opened CS_P4_J303_sharp_flip.mrc as #49, grid size 80,80,80, pixel 3.32, shown
at level 0.575, step 1, values float32
Opened CS_P4_J304_sharp_flip.mrc as #50, grid size 80,80,80, pixel 3.32, shown
at level 1.95, step 1, values float32
Opened CS_P4_J305_sharp_flip.mrc as #51, grid size 80,80,80, pixel 3.32, shown
at level 0.569, step 1, values float32
Opened CS_P4_J306_sharp_flip.mrc as #52, grid size 80,80,80, pixel 3.32, shown
at level 1.03, step 1, values float32
Opened CS_P4_J307_sharp_flip.mrc as #53, grid size 80,80,80, pixel 3.32, shown
at level 0.417, step 1, values float32
Opened CS_P4_J308_sharp_flip.mrc as #54, grid size 80,80,80, pixel 3.32, shown
at level 0.487, step 1, values float32
Opened CS_P4_J309_sharp_flip.mrc as #55, grid size 80,80,80, pixel 3.32, shown
at level 0.427, step 1, values float32
Opened CS_P4_J310_sharp_flip.mrc as #56, grid size 80,80,80, pixel 3.32, shown
at level 0.719, step 1, values float32
Opened CS_P4_J311_sharp_flip.mrc as #57, grid size 80,80,80, pixel 3.32, shown
at level 0.525, step 1, values float32
Log from Wed May 6 15:19:28 2026UCSF ChimeraX version: 1.11 (2025-12-17)
© 2016-2025 Regents of the University of California. All rights reserved.
> open "/Users/peiyuyu/Documents/Goethe
> University/2026/5_Manuscripts/1_AcrF/Figure/Figure_cryoEM_workflow/SA-
> F_B1/20260505_SA-F_B1_workflow.cxs"
Opened cryosparc_P4_J216_class_03_00122_volume_sharp.mrc as #4, grid size
128,128,128, pixel 2.08, shown at level 0.203, step 1, values float32
Opened cryosparc_P4_J216_class_04_00122_volume_sharp.mrc as #5, grid size
128,128,128, pixel 2.08, shown at level 0.206, step 1, values float32
Opened CS_P4_J216_class_0_sharp_flip.mrc as #1, grid size 128,128,128, pixel
2.08, shown at level 0.185, step 1, values float32
Opened CS_P4_J216_class_1_sharp_flip.mrc as #2, grid size 128,128,128, pixel
2.08, shown at level 0.203, step 1, values float32
Opened CS_P4_J216_class_2_sharp_flip.mrc as #3, grid size 128,128,128, pixel
2.08, shown at level 0.356, step 1, values float32
Opened CS_P4_J219_sharp_flip.mrc as #6, grid size 80,80,80, pixel 3.32, shown
at level 1.17, step 1, values float32
Opened CS_P4_J244_sharp_flip.mrc as #7, grid size 80,80,80, pixel 3.32, shown
at level 1.16, step 1, values float32
Opened cryosparc_P4_J242_class_00_00122_volume_sharp.mrc as #8, grid size
128,128,128, pixel 2.08, shown at level 0.221, step 1, values float32
Opened cryosparc_P4_J242_class_01_00122_volume_sharp.mrc as #9, grid size
128,128,128, pixel 2.08, shown at level 0.146, step 1, values float32
Opened cryosparc_P4_J242_class_02_00122_volume_sharp.mrc as #10, grid size
128,128,128, pixel 2.08, shown at level 0.195, step 1, values float32
Opened cryosparc_P4_J242_class_03_00122_volume_sharp.mrc as #11, grid size
128,128,128, pixel 2.08, shown at level 0.15, step 1, values float32
Opened cryosparc_P4_J242_class_04_00122_volume_sharp.mrc as #12, grid size
128,128,128, pixel 2.08, shown at level 0.345, step 1, values float32
Opened cryosparc_P4_J117_class_00_00162_volume_sharp.mrc as #13, grid size
128,128,128, pixel 2.08, shown at level 0.163, step 1, values float32
Opened cryosparc_P4_J117_class_01_00162_volume_sharp.mrc as #14, grid size
128,128,128, pixel 2.08, shown at level 0.187, step 1, values float32
Opened CS_P4_J117_class_02_sharp_flip.mrc as #15, grid size 128,128,128, pixel
2.08, shown at level 0.335, step 1, values float32
Opened cryosparc_P4_J117_class_03_00162_volume_sharp.mrc as #16, grid size
128,128,128, pixel 2.08, shown at level 0.179, step 1, values float32
Opened cryosparc_P4_J117_class_04_00162_volume_sharp.mrc as #17, grid size
128,128,128, pixel 2.08, shown at level 0.206, step 1, values float32
Opened CS_P4_J120_sharp_flip.mrc as #18, grid size 80,80,80, pixel 3.32, shown
at level 1.29, step 1, values float32
Opened cryosparc_P4_J169_class_00_00182_volume_sharp.mrc as #19, grid size
128,128,128, pixel 2.08, shown at level 0.156, step 1, values float32
Opened cryosparc_P4_J169_class_01_00182_volume_sharp.mrc as #20, grid size
128,128,128, pixel 2.08, shown at level 0.198, step 1, values float32
Opened cryosparc_P4_J169_class_02_00182_volume_sharp.mrc as #21, grid size
128,128,128, pixel 2.08, shown at level 0.248, step 1, values float32
Opened CS_P4_J169_class_03_sharp_flip.mrc as #22, grid size 128,128,128, pixel
2.08, shown at level 0.271, step 1, values float32
Opened cryosparc_P4_J169_class_04_00182_volume_sharp.mrc as #23, grid size
128,128,128, pixel 2.08, shown at level 0.2, step 1, values float32
Opened CS_P4_J176_NU_sharp_flip.mrc as #24, grid size 80,80,80, pixel 3.32,
shown at level 1.6, step 1, values float32
Opened CS_P4_J255_sharp_flip.mrc as #25, grid size 80,80,80, pixel 3.32, shown
at level 0.649, step 1, values float32
Opened CS_P4_J256_sharp_flip.mrc as #26, grid size 80,80,80, pixel 3.32, shown
at level 1.01, step 1, values float32
Opened CS_P4_J257_sharp_flip.mrc as #27, grid size 80,80,80, pixel 3.32, shown
at level 1.16, step 1, values float32
Opened CS_P4_J258_sharp_flip.mrc as #28, grid size 80,80,80, pixel 3.32, shown
at level 0.533, step 1, values float32
Opened CS_P4_J259_sharp_flip.mrc as #29, grid size 80,80,80, pixel 3.32, shown
at level 0.567, step 1, values float32
Opened CS_P4_J267_sharp_flip.mrc as #30, grid size 320,320,320, pixel 0.831,
shown at level 0.134, step 1, values float32
Opened CS_P4_J277_sharp_flip.mrc as #31, grid size 320,320,320, pixel 0.831,
shown at level 0.143, step 1, values float32
Opened cryosparc_P4_J282_005_volume_map_sharp.mrc as #32, grid size
320,320,320, pixel 0.831, shown at level 0.0548, step 2, values float32
Opened cryosparc_P4_J285_006_volume_map_sharp.mrc as #33, grid size
320,320,320, pixel 0.831, shown at level 0.0508, step 2, values float32
Opened cryosparc_P4_J287_map_locres.mrc as #34, grid size 320,320,320, pixel
0.831, shown at level 3.78, step 2, values float32
Opened cryosparc_P4_J288_map_locres.mrc as #35, grid size 320,320,320, pixel
0.831, shown at level 3.77, step 2, values float32
Opened cryosparc_P4_J282_005_volume_map_sharp.mrc z flip as #37, grid size
320,320,320, pixel 0.831, shown at level 0.065, step 1, values float32
Opened cryosparc_P4_J287_map_locres.mrc z flip as #38, grid size 320,320,320,
pixel 0.831, shown at level 3.78, step 1, values float32
Opened cryosparc_P4_J285_006_volume_map_sharp.mrc z flip as #39, grid size
320,320,320, pixel 0.831, shown at level 0.0664, step 1, values float32
Opened cryosparc_P4_J288_map_locres.mrc z flip as #40, grid size 320,320,320,
pixel 0.831, shown at level 3.77, step 1, values float32
Opened cryosparc_P4_J242_class_01_00122_volume_sharp.mrc as #42, grid size
128,128,128, pixel 2.08, shown at level 0.254, step 1, values float32
Opened CS_P4_J282_sharp_flip.mrc as #36, grid size 320,320,320, pixel 0.831,
shown at level 0.0602, step 2, values float32
Opened cryosparc_P4_J169_class_02_00182_volume_sharp.mrc as #43, grid size
128,128,128, pixel 2.08, shown at level 0.228, step 1, values float32
Opened CS_P4_J295_sharp_flip.mrc as #44, grid size 320,320,320, pixel 0.831,
shown at level 0.0515, step 1, values float32
Opened CS_P4_J296_locres_flip.mrc as #45, grid size 320,320,320, pixel 0.831,
shown at level 8.66, step 1, values float32
Opened CS_P4_J300_sharp_flip.mrc as #46, grid size 320,320,320, pixel 0.831,
shown at level 0.0592, step 1, values float32
Opened CS_P4_J301_locres_flip.mrc as #47, grid size 320,320,320, pixel 0.831,
shown at level 9.68, step 1, values float32
Log from Tue May 5 09:31:57 2026UCSF ChimeraX version: 1.11 (2025-12-17)
© 2016-2025 Regents of the University of California. All rights reserved.
> open "/Users/peiyuyu/Documents/Goethe
> University/2026/5_Manuscripts/1_AcrF/Figure/Figure_cryoEM_workflow/SA-
> F_B1/20260503_SA-F_B1_workflow4.cxs"
Opened cryosparc_P4_J216_class_03_00122_volume_sharp.mrc as #4, grid size
128,128,128, pixel 2.08, shown at level 0.203, step 1, values float32
Opened cryosparc_P4_J216_class_04_00122_volume_sharp.mrc as #5, grid size
128,128,128, pixel 2.08, shown at level 0.206, step 1, values float32
Opened CS_P4_J216_class_0_sharp_flip.mrc as #1, grid size 128,128,128, pixel
2.08, shown at level 0.185, step 1, values float32
Opened CS_P4_J216_class_1_sharp_flip.mrc as #2, grid size 128,128,128, pixel
2.08, shown at level 0.203, step 1, values float32
Opened CS_P4_J216_class_2_sharp_flip.mrc as #3, grid size 128,128,128, pixel
2.08, shown at level 0.356, step 1, values float32
Opened CS_P4_J219_sharp_flip.mrc as #6, grid size 80,80,80, pixel 3.32, shown
at level 1.17, step 1, values float32
Opened CS_P4_J244_sharp_flip.mrc as #7, grid size 80,80,80, pixel 3.32, shown
at level 1.16, step 1, values float32
Opened cryosparc_P4_J242_class_00_00122_volume_sharp.mrc as #8, grid size
128,128,128, pixel 2.08, shown at level 0.221, step 1, values float32
Opened cryosparc_P4_J242_class_01_00122_volume_sharp.mrc as #9, grid size
128,128,128, pixel 2.08, shown at level 0.146, step 1, values float32
Opened cryosparc_P4_J242_class_02_00122_volume_sharp.mrc as #10, grid size
128,128,128, pixel 2.08, shown at level 0.195, step 1, values float32
Opened cryosparc_P4_J242_class_03_00122_volume_sharp.mrc as #11, grid size
128,128,128, pixel 2.08, shown at level 0.15, step 1, values float32
Opened cryosparc_P4_J242_class_04_00122_volume_sharp.mrc as #12, grid size
128,128,128, pixel 2.08, shown at level 0.345, step 1, values float32
Opened cryosparc_P4_J117_class_00_00162_volume_sharp.mrc as #13, grid size
128,128,128, pixel 2.08, shown at level 0.163, step 1, values float32
Opened cryosparc_P4_J117_class_01_00162_volume_sharp.mrc as #14, grid size
128,128,128, pixel 2.08, shown at level 0.187, step 1, values float32
Opened CS_P4_J117_class_02_sharp_flip.mrc as #15, grid size 128,128,128, pixel
2.08, shown at level 0.335, step 1, values float32
Opened cryosparc_P4_J117_class_03_00162_volume_sharp.mrc as #16, grid size
128,128,128, pixel 2.08, shown at level 0.179, step 1, values float32
Opened cryosparc_P4_J117_class_04_00162_volume_sharp.mrc as #17, grid size
128,128,128, pixel 2.08, shown at level 0.206, step 1, values float32
Opened CS_P4_J120_sharp_flip.mrc as #18, grid size 80,80,80, pixel 3.32, shown
at level 1.29, step 1, values float32
Opened cryosparc_P4_J169_class_00_00182_volume_sharp.mrc as #19, grid size
128,128,128, pixel 2.08, shown at level 0.156, step 1, values float32
Opened cryosparc_P4_J169_class_01_00182_volume_sharp.mrc as #20, grid size
128,128,128, pixel 2.08, shown at level 0.198, step 1, values float32
Opened cryosparc_P4_J169_class_02_00182_volume_sharp.mrc as #21, grid size
128,128,128, pixel 2.08, shown at level 0.248, step 1, values float32
Opened CS_P4_J169_class_03_sharp_flip.mrc as #22, grid size 128,128,128, pixel
2.08, shown at level 0.271, step 1, values float32
Opened cryosparc_P4_J169_class_04_00182_volume_sharp.mrc as #23, grid size
128,128,128, pixel 2.08, shown at level 0.2, step 1, values float32
Opened CS_P4_J176_NU_sharp_flip.mrc as #24, grid size 80,80,80, pixel 3.32,
shown at level 1.6, step 1, values float32
Opened CS_P4_J255_sharp_flip.mrc as #25, grid size 80,80,80, pixel 3.32, shown
at level 0.649, step 1, values float32
Opened CS_P4_J256_sharp_flip.mrc as #26, grid size 80,80,80, pixel 3.32, shown
at level 1.01, step 1, values float32
Opened CS_P4_J257_sharp_flip.mrc as #27, grid size 80,80,80, pixel 3.32, shown
at level 1.16, step 1, values float32
Opened CS_P4_J258_sharp_flip.mrc as #28, grid size 80,80,80, pixel 3.32, shown
at level 0.533, step 1, values float32
Opened CS_P4_J259_sharp_flip.mrc as #29, grid size 80,80,80, pixel 3.32, shown
at level 0.567, step 1, values float32
Opened CS_P4_J267_sharp_flip.mrc as #30, grid size 320,320,320, pixel 0.831,
shown at level 0.134, step 1, values float32
Opened CS_P4_J277_sharp_flip.mrc as #31, grid size 320,320,320, pixel 0.831,
shown at level 0.143, step 1, values float32
Opened cryosparc_P4_J282_005_volume_map_sharp.mrc as #32, grid size
320,320,320, pixel 0.831, shown at level 0.0548, step 2, values float32
Opened cryosparc_P4_J285_006_volume_map_sharp.mrc as #33, grid size
320,320,320, pixel 0.831, shown at level 0.0508, step 2, values float32
Opened cryosparc_P4_J287_map_locres.mrc as #34, grid size 320,320,320, pixel
0.831, shown at level 3.78, step 2, values float32
Opened cryosparc_P4_J288_map_locres.mrc as #35, grid size 320,320,320, pixel
0.831, shown at level 3.77, step 2, values float32
Opened cryosparc_P4_J282_005_volume_map_sharp.mrc z flip as #37, grid size
320,320,320, pixel 0.831, shown at level 0.065, step 1, values float32
Opened cryosparc_P4_J287_map_locres.mrc z flip as #38, grid size 320,320,320,
pixel 0.831, shown at level 3.78, step 1, values float32
Opened cryosparc_P4_J285_006_volume_map_sharp.mrc z flip as #39, grid size
320,320,320, pixel 0.831, shown at level 0.0664, step 1, values float32
Opened cryosparc_P4_J288_map_locres.mrc z flip as #40, grid size 320,320,320,
pixel 0.831, shown at level 3.77, step 1, values float32
Opened cryosparc_P4_J242_class_01_00122_volume_sharp.mrc as #42, grid size
128,128,128, pixel 2.08, shown at level 0.254, step 1, values float32
Opened CS_P4_J282_sharp_flip.mrc as #36, grid size 320,320,320, pixel 0.831,
shown at level 0.0602, step 2, values float32
Opened cryosparc_P4_J169_class_02_00182_volume_sharp.mrc as #43, grid size
128,128,128, pixel 2.08, shown at level 0.228, step 1, values float32
Log from Sun May 3 17:29:06 2026UCSF ChimeraX version: 1.11 (2025-12-17)
© 2016-2025 Regents of the University of California. All rights reserved.
How to cite UCSF ChimeraX
> open "/Users/peiyuyu/Documents/Goethe
> University/2026/5_Manuscripts/1_AcrF/Figure/Figure_cryoEM_workflow/SA-
> F_B1/cryosparc_P4_J216_class_00_00122_volume_sharp.mrc"
Opened cryosparc_P4_J216_class_00_00122_volume_sharp.mrc as #1, grid size
128,128,128, pixel 2.08, shown at level 0.204, step 1, values float32
> open "/Users/peiyuyu/Documents/Goethe
> University/2026/5_Manuscripts/1_AcrF/Figure/Figure_cryoEM_workflow/SA-
> F_B1/cryosparc_P4_J216_class_01_00122_volume_sharp.mrc"
Opened cryosparc_P4_J216_class_01_00122_volume_sharp.mrc as #2, grid size
128,128,128, pixel 2.08, shown at level 0.218, step 1, values float32
> open "/Users/peiyuyu/Documents/Goethe
> University/2026/5_Manuscripts/1_AcrF/Figure/Figure_cryoEM_workflow/SA-
> F_B1/cryosparc_P4_J216_class_02_00122_volume_sharp.mrc"
Opened cryosparc_P4_J216_class_02_00122_volume_sharp.mrc as #3, grid size
128,128,128, pixel 2.08, shown at level 0.263, step 1, values float32
> open "/Users/peiyuyu/Documents/Goethe
> University/2026/5_Manuscripts/1_AcrF/Figure/Figure_cryoEM_workflow/SA-
> F_B1/cryosparc_P4_J216_class_03_00122_volume_sharp.mrc"
Opened cryosparc_P4_J216_class_03_00122_volume_sharp.mrc as #4, grid size
128,128,128, pixel 2.08, shown at level 0.199, step 1, values float32
> open "/Users/peiyuyu/Documents/Goethe
> University/2026/5_Manuscripts/1_AcrF/Figure/Figure_cryoEM_workflow/SA-
> F_B1/cryosparc_P4_J216_class_04_00122_volume_sharp.mrc"
Opened cryosparc_P4_J216_class_04_00122_volume_sharp.mrc as #5, grid size
128,128,128, pixel 2.08, shown at level 0.235, step 1, values float32
> lighting soft
> set bgColor white
> hide #!5 models
> hide #!4 models
Deeleted thousands of lines to meet Trac size limit. Tom
> view matrix models #43,1,0,0,-10.922,0,1,0,105.75,0,0,1,25.23
> ui mousemode right "rotate selected models"
> view matrix models
> #43,0.053361,-0.96815,-0.24461,270.41,-0.10024,-0.24891,0.96332,141.36,-0.99353,-0.026885,-0.11033,315.47
> ui mousemode right "translate selected models"
> view matrix models
> #43,0.053361,-0.96815,-0.24461,276.43,-0.10024,-0.24891,0.96332,50.344,-0.99353,-0.026885,-0.11033,257.37
> ui mousemode right "rotate selected models"
> view matrix models
> #43,0.25016,-0.91768,-0.30868,252.37,-0.0074281,-0.32063,0.94718,48.872,-0.96818,-0.23465,-0.087026,276.42
> ui mousemode right "translate selected models"
> view matrix models
> #43,0.25016,-0.91768,-0.30868,252.17,-0.0074281,-0.32063,0.94718,49.119,-0.96818,-0.23465,-0.087026,280.28
> view matrix models
> #43,0.25016,-0.91768,-0.30868,245.18,-0.0074281,-0.32063,0.94718,41.479,-0.96818,-0.23465,-0.087026,278.46
> view matrix models
> #43,0.25016,-0.91768,-0.30868,244.24,-0.0074281,-0.32063,0.94718,46.881,-0.96818,-0.23465,-0.087026,278.05
> select add #42
4 models selected
> select subtract #43
2 models selected
> view matrix models
> #42,0.15189,-0.98482,-0.084022,248.26,-0.056394,-0.093504,0.99402,10.768,-0.98679,-0.14625,-0.069741,272.27
> select subtract #42
Nothing selected
> color #36 #99BFE5
> hide #!42 models
> select add #43
2 models selected
> ui mousemode right "rotate selected models"
> view matrix models
> #43,0.073784,-0.55439,0.82898,66.918,0.0052521,0.83145,0.55558,-43.45,-0.99726,-0.036639,0.064259,236.72
> ui mousemode right "translate selected models"
> view matrix models
> #43,0.073784,-0.55439,0.82898,82.905,0.0052521,0.83145,0.55558,-39.115,-0.99726,-0.036639,0.064259,236.78
> select subtract #43
Nothing selected
> volume #36 level 0.0602
> select add #43
2 models selected
> view matrix models
> #43,0.073784,-0.55439,0.82898,84.941,0.0052521,0.83145,0.55558,-38.546,-0.99726,-0.036639,0.064259,236.53
> view matrix models
> #43,0.073784,-0.55439,0.82898,84.833,0.0052521,0.83145,0.55558,-38.074,-0.99726,-0.036639,0.064259,236.34
> volume #43 level 0.1902
> color #43 #b2b2ff80 models
> volume #43 level 0.2283
> select add #40
4 models selected
> select subtract #40
2 models selected
> select subtract #43
Nothing selected
> color #43 #ffffb280 models
> color #43 #b2b2ff80 models
> save "/Users/peiyuyu/Documents/Goethe
> University/2026/5_Manuscripts/1_AcrF/Figure/Figure_cryoEM_workflow/SA-
> F_B1/20260503_SA-F_B1_workflow3.cxs"
> open "/Users/peiyuyu/Documents/Goethe
> University/2026/5_Manuscripts/1_AcrF/Figure/Figure_cryoEM_workflow/SA-
> F_B1/cryosparc_P4_cryosparc_P4_J243_003_volume_map_35_TMD_regions_resampled.mrc"
Opened
cryosparc_P4_cryosparc_P4_J243_003_volume_map_35_TMD_regions_resampled.mrc as
#44, grid size 80,80,80, pixel 3.32, shown at level 0.324, step 1, values
float32
> close #44
> save CS_P4_J282_show_mask_sharp.png supersample 3 transparentBackground true
> save "/Users/peiyuyu/Documents/Goethe
> University/2026/5_Manuscripts/1_AcrF/Figure/Figure_cryoEM_workflow/SA-
> F_B1/20260503_SA-F_B1_workflow4.cxs"
——— End of log from Sun May 3 17:29:06 2026 ———
> view name session-start
opened ChimeraX session
> open "/Users/peiyuyu/Documents/Goethe
> University/2026/5_Manuscripts/1_AcrF/Figure/Figure_cryoEM_workflow/SA-
> F_B1/cryosparc_P4_J295_006_volume_map_sharp.mrc"
Opened cryosparc_P4_J295_006_volume_map_sharp.mrc as #44, grid size
320,320,320, pixel 0.831, shown at level 0.0361, step 2, values float32
> open "/Users/peiyuyu/Documents/Goethe
> University/2026/5_Manuscripts/1_AcrF/Figure/Figure_cryoEM_workflow/SA-
> F_B1/cryosparc_P4_J296_map_locres.mrc"
Opened cryosparc_P4_J296_map_locres.mrc as #45, grid size 320,320,320, pixel
0.831, shown at level 8.66, step 2, values float32
> hide #!43 models
> hide #!36 models
> hide #!45 models
> show #!45 models
> hide #!45 models
> volume flip #44
Opened cryosparc_P4_J295_006_volume_map_sharp.mrc z flip as #46, grid size
320,320,320, pixel 0.831, shown at step 1, values float32
> volume flip #45
Opened cryosparc_P4_J296_map_locres.mrc z flip as #47, grid size 320,320,320,
pixel 0.831, shown at step 1, values float32
> close #45
> close #44
> rename #46 CS_P4_J295_sharp_flip id #44
> save CS_P4_J295_sharp_flip.mrc #44
> rename #47 CS_P4_J296_locres_flip id #45
> save CS_P4_J296_locres_flip.mrc #45
> ui tool show "Surface Color"
> hide #!45 models
> color sample #44.1 map #45 palette #ffb17f:#527dea:#99bfe5:#fff6c0:#f4fff3
> outsideColor #808080
Map values for surface "surface": minimum 0, mean 4.132, maximum 48.35
> show #41 models
> volume #44 level 0.0515
> color sample #44.1 map #45 palette
> 1,#ffb17f:2,#527dea:3,#99bfe5:4,#fff6c0:5,#f4fff3 outsideColor #808080
Map values for surface "surface": minimum 0, mean 3.583, maximum 39.22
> color sample #44.1 map #45 palette
> 2,#ffb17f:3,#527dea:4,#99bfe5:5,#fff6c0:6,#f4fff3 outsideColor #808080
Map values for surface "surface": minimum 0, mean 3.583, maximum 39.22
> color sample #44.1 map #45 palette
> 2,#ffb17f:3,#527dea:4,#ffb17f:5,#fff6c0:6,#f4fff3 outsideColor #808080
Map values for surface "surface": minimum 0, mean 3.583, maximum 39.22
> color sample #44.1 map #45 palette
> 2,#527dea:3,#99bfe5:4,#ffb17f:5,#fff6c0:6,#f4fff3 outsideColor #808080
Map values for surface "surface": minimum 0, mean 3.583, maximum 39.22
> key #527dea:2 #99bfe5:3 #ffb17f:4 #fff6c0:5 #f4fff3:6 showTool true
> ui mousemode right "color key"
> color sample #44.1 map #45 palette
> 2,#f4fff3:3,#fff6c0:4,#ffb17f:5,#99bfe5:6,#527dea outsideColor #808080
Map values for surface "surface": minimum 0, mean 3.583, maximum 39.22
> key #f4fff3:2 #fff6c0:3 #ffb17f:4 #99bfe5:5 #527dea:6 showTool true
> ui mousemode right "color key"
> color sample #44.1 map #45 palette
> 2,#f4fff3:3,#fff6c0:4,#ffb17f:5,#99bfe5:6,#527dd6 outsideColor #808080
Map values for surface "surface": minimum 0, mean 3.583, maximum 39.22
> color sample #44.1 map #45 palette
> 2,#f4fff3:3,#fff6c0:4,#ffb17f:5,#99bfe5:6,#46a2de outsideColor #808080
Map values for surface "surface": minimum 0, mean 3.583, maximum 39.22
> color sample #44.1 map #45 palette
> 2,#f4fff3:3,#fff6c0:4,#ffb17f:5,#99bfe5:6,#4d8fde outsideColor #808080
Map values for surface "surface": minimum 0, mean 3.583, maximum 39.22
> color sample #44.1 map #45 palette
> 2,#f4fff3:3,#fff6c0:4,#ffb17f:5,#99bfe5:6,#94aec1 outsideColor #808080
Map values for surface "surface": minimum 0, mean 3.583, maximum 39.22
> color sample #44.1 map #45 palette
> 2,#f4fff3:3,#fff6c0:4,#ffb17f:5,#99bfe5:6,#a78bc1 outsideColor #808080
Map values for surface "surface": minimum 0, mean 3.583, maximum 39.22
> color sample #44.1 map #45 palette
> 2,#f4fff3:3,#fff6c0:4,#ffb17f:5,#99bfe5:6,#c1a77f outsideColor #808080
Map values for surface "surface": minimum 0, mean 3.583, maximum 39.22
> color sample #44.1 map #45 palette
> 2,#f4fff3:3,#fff6c0:4,#ffb17f:5,#99bfe5:6,#89b3c1 outsideColor #808080
Map values for surface "surface": minimum 0, mean 3.583, maximum 39.22
> color sample #44.1 map #45 palette
> 2,#ffb17f:3,#527dea:4,#99bfe5:5,#fff6c0:6,#f4fff3 outsideColor #808080
Map values for surface "surface": minimum 0, mean 3.583, maximum 39.22
> key #ffb17f:2 #527dea:3 #99bfe5:4 #fff6c0:5 #f4fff3:6 showTool true
> ui mousemode right "color key"
> color sample #44.1 map #45 palette
> 2,#ffb17f:3,#2c98ee:4,#99bfe5:5,#fff6c0:6,#f4fff3 outsideColor #808080
Map values for surface "surface": minimum 0, mean 3.583, maximum 39.22
> color sample #44.1 map #45 palette
> 2,#ffb17f:3,#4979d3:4,#99bfe5:5,#fff6c0:6,#f4fff3 outsideColor #808080
Map values for surface "surface": minimum 0, mean 3.583, maximum 39.22
> save CS_P4_J295_locres_sharp.png supersample 3 transparentBackground true
Cell requested for row 0 is out of bounds for table with 6 rows! Resizing
table model.
> save "/Users/peiyuyu/Documents/Goethe
> University/2026/5_Manuscripts/1_AcrF/Figure/Figure_cryoEM_workflow/SA-
> F_B1/20260505_SA-F_B1_workflow.cxs"
> open "/Users/peiyuyu/Documents/Goethe
> University/2026/5_Manuscripts/1_AcrF/Figure/Figure_cryoEM_workflow/SA-
> F_B1/cryosparc_P4_J300_005_volume_map_sharp.mrc"
Opened cryosparc_P4_J300_005_volume_map_sharp.mrc as #46, grid size
320,320,320, pixel 0.831, shown at level 0.0375, step 2, values float32
> open "/Users/peiyuyu/Documents/Goethe
> University/2026/5_Manuscripts/1_AcrF/Figure/Figure_cryoEM_workflow/SA-
> F_B1/cryosparc_P4_J301_map_locres.mrc"
Opened cryosparc_P4_J301_map_locres.mrc as #47, grid size 320,320,320, pixel
0.831, shown at level 9.68, step 2, values float32
> hide #!44 models
> hide #!47 models
> volume flip #46
Opened cryosparc_P4_J300_005_volume_map_sharp.mrc z flip as #48, grid size
320,320,320, pixel 0.831, shown at step 1, values float32
> volume flip #47
Opened cryosparc_P4_J301_map_locres.mrc z flip as #49, grid size 320,320,320,
pixel 0.831, shown at step 1, values float32
> close #46
> close #47
> rename #48 CS_P4_J300_sharp_flip id #46
> rename #49 CS_P4_J301_locres_flip id #47
> save CS_P4_J301_locres_flip.mrc #47
> save CS_P4_J300_sharp_flip.mrc #46
> color sample #46.1 map #47 palette
> 2,#ffb17f:3,#4979d3:4,#99bfe5:5,#fff6c0:6,#f4fff3 outsideColor #808080
Map values for surface "surface": minimum 0, mean 4.498, maximum 44.88
> hide #!47 models
> volume #46 level 0.03399
> volume #46 level 0.06962
> volume #46 level 0.05919
> save CS_P4_J300_locres_sharp.png supersample 3 transparentBackground true
> save "/Users/peiyuyu/Documents/Goethe
> University/2026/5_Manuscripts/1_AcrF/Figure/Figure_cryoEM_workflow/SA-
> F_B1/20260505_SA-F_B1_workflow.cxs"
——— End of log from Tue May 5 09:31:57 2026 ———
> view name session-start
opened ChimeraX session
> open "/Users/peiyuyu/Documents/Goethe
> University/2026/5_Manuscripts/1_AcrF/Figure/Figure_cryoEM_workflow/SA-
> F_B1/cryosparc_P4_J302_002_volume_map_sharp.mrc"
Opened cryosparc_P4_J302_002_volume_map_sharp.mrc as #48, grid size 80,80,80,
pixel 3.32, shown at level 0.658, step 1, values float32
> select add #48
2 models selected
> ui mousemode right "rotate selected models"
> view matrix models
> #48,0.064602,0.97911,0.19278,-26.266,0.98732,-0.034646,-0.1549,22.036,-0.14498,0.20034,-0.96894,229.94
> volume flip #48
Opened cryosparc_P4_J302_002_volume_map_sharp.mrc z flip as #49, grid size
80,80,80, pixel 3.32, shown at step 1, values float32
> close #48
> rename #49 rename
> rename #49 CS_P4_J302_sharp_flip id #48
> save CS_P4_J302_sharp_flip.mrc #48
> select add #48
2 models selected
> view matrix models
> #48,-0.56486,-0.82475,0.026783,324.58,0.77395,-0.54076,-0.32951,143.72,0.28625,-0.1654,0.94377,-52.222
> hide #41 models
> hide #!46 models
> show #!47 models
> hide #!47 models
> show #!45 models
> hide #!45 models
> show #!43 models
> hide #!43 models
> show #!39 models
> ui mousemode right "translate selected models"
> view matrix models
> #48,-0.56486,-0.82475,0.026783,319.51,0.77395,-0.54076,-0.32951,139.04,0.28625,-0.1654,0.94377,-3.8614
> view matrix models
> #48,-0.56486,-0.82475,0.026783,321.32,0.77395,-0.54076,-0.32951,140.03,0.28625,-0.1654,0.94377,-10.821
> fitmap $48 inmap #39
Missing or invalid "atomsOrMap" argument: invalid objects specifier
> fitmap #48 inMap #39
Fit map CS_P4_J302_sharp_flip.mrc in map
cryosparc_P4_J285_006_volume_map_sharp.mrc z flip using 5112 points
correlation = 0.3608, correlation about mean = 0.02111, overlap = 103.8
steps = 76, shift = 4.71, angle = 3.71 degrees
Position of CS_P4_J302_sharp_flip.mrc (#48) relative to
cryosparc_P4_J285_006_volume_map_sharp.mrc z flip (#39) coordinates:
Matrix rotation and translation
-0.54436883 -0.83749052 0.04766738 317.13056079
0.79848148 -0.53475206 -0.27652762 129.04232256
0.25707950 -0.11247150 0.95982304 -11.58663120
Axis 0.09898047 -0.12634523 0.98703584
Axis point 124.28808276 149.04371086 0.00000000
Rotation angle (degrees) 124.03152255
Shift along axis 3.64942894
> ui mousemode right "rotate selected models"
> view matrix models
> #48,-0.38435,0.91427,-0.12797,86.018,-0.92117,-0.38898,-0.012326,307,-0.061046,0.11314,0.9917,-2.8083
> fitmap #48 inMap #39
Fit map CS_P4_J302_sharp_flip.mrc in map
cryosparc_P4_J285_006_volume_map_sharp.mrc z flip using 5112 points
correlation = 0.623, correlation about mean = 0.1973, overlap = 254
steps = 104, shift = 5.55, angle = 8.62 degrees
Position of CS_P4_J302_sharp_flip.mrc (#48) relative to
cryosparc_P4_J285_006_volume_map_sharp.mrc z flip (#39) coordinates:
Matrix rotation and translation
-0.49090927 0.85957769 -0.14189522 105.36638005
-0.85910240 -0.50468247 -0.08508012 328.20339808
-0.14474501 0.08013591 0.98621858 12.70754555
Axis 0.09568836 0.00165051 -0.99540997
Axis point 147.88121154 133.69042072 0.00000000
Rotation angle (degrees) 120.31054935
Shift along axis -2.02517664
> select subtract #48
Nothing selected
> volume #48 level 0.8066
> hide #!48 models
> show #!48 models
> hide #!39 models
> show #!40 models
> hide #!40 models
> show #!46 models
> hide #!46 models
> show #!46 models
> hide #!46 models
> volume #48 level 0.6121
> show #!24 models
> hide #!24 models
> color #48 #99BFE5
> hide #!48 models
> show #!48 models
> open "/Users/peiyuyu/Documents/Goethe
> University/2026/5_Manuscripts/1_AcrF/Figure/Figure_cryoEM_workflow/SA-
> F_B1/cryosparc_P4_J303_003_volume_map_sharp.mrc"
Opened cryosparc_P4_J303_003_volume_map_sharp.mrc as #49, grid size 80,80,80,
pixel 3.32, shown at level 0.57, step 1, values float32
> select add #49
2 models selected
> view matrix models
> #49,0.53874,0.84077,0.053573,-61.438,0.83514,-0.54134,0.097405,87.116,0.1109,-0.0077344,-0.9938,260.56
> view matrix models
> #49,0.53215,0.84494,0.053856,-61.154,0.83957,-0.53484,0.095262,85.896,0.10929,-0.0054773,-0.99399,260.49
> view matrix models
> #49,-0.8763,-0.44891,0.17485,296.18,-0.45964,0.88777,-0.024369,82.131,-0.14428,-0.10172,-0.98429,307.41
> volume flip #49
Opened cryosparc_P4_J303_003_volume_map_sharp.mrc z flip as #50, grid size
80,80,80, pixel 3.32, shown at step 1, values float32
> close #49
> rename #50 CS_P4_J303_sharp_flip id #49
> save CS_P4_J303_sharp_flip.mrc #49
> select add #49
2 models selected
> view matrix models
> #49,-0.44927,0.88595,-0.11509,87.376,-0.86051,-0.46376,-0.21085,349.13,-0.24018,0.0043123,0.97072,61.749
> ui mousemode right "translate selected models"
> view matrix models
> #49,-0.44927,0.88595,-0.11509,93.572,-0.86051,-0.46376,-0.21085,352.06,-0.24018,0.0043123,0.97072,37.843
> fitmap #49 inMap 348
Invalid "inMap" argument: invalid density map specifier
> fitmap #49 inMap #48
Fit map CS_P4_J303_sharp_flip.mrc in map CS_P4_J302_sharp_flip.mrc using 5118
points
correlation = 0.8483, correlation about mean = 0.4386, overlap = 3011
steps = 68, shift = 10.7, angle = 7.19 degrees
Position of CS_P4_J303_sharp_flip.mrc (#49) relative to
CS_P4_J302_sharp_flip.mrc (#48) coordinates:
Matrix rotation and translation
0.99996126 -0.00401528 0.00783497 -1.22617333
0.00399514 0.99998870 0.00258400 -1.26181412
-0.00784526 -0.00255260 0.99996598 1.34185977
Axis -0.28004899 0.85489100 0.43673098
Axis point 178.64594040 0.00000000 162.72002408
Rotation angle (degrees) 0.52546068
Shift along axis -0.14929320
> select subtract #49
Nothing selected
> hide #!48 models
> color #49 #FCEDFF
> hide #!49 models
> show #!49 models
> volume #49 level 0.5753
> open "/Users/peiyuyu/Documents/Goethe
> University/2026/5_Manuscripts/1_AcrF/Figure/Figure_cryoEM_workflow/SA-
> F_B1/cryosparc_P4_J304_002_volume_map_sharp.mrc"
Opened cryosparc_P4_J304_002_volume_map_sharp.mrc as #50, grid size 80,80,80,
pixel 3.32, shown at level 1.66, step 1, values float32
> hide #!49 models
> select add #50
2 models selected
> ui mousemode right "rotate selected models"
> view matrix models
> #50,0.25626,0.96633,0.023099,-33.544,0.9662,-0.25539,-0.035012,44.222,-0.027934,0.03129,-0.99912,256.23
> volume #50 level 2.579
> select subtract #50
Nothing selected
> volume #50 level 1.975
> volume flip #50
Opened cryosparc_P4_J304_002_volume_map_sharp.mrc z flip as #51, grid size
80,80,80, pixel 3.32, shown at step 1, values float32
> close #50
> rename #51 CS_P4_J304_sharp_flip id #50
> save CS_P4_J304_sharp_flip.mrc #50
> select add #50
2 models selected
> show #!49 models
> view matrix models
> #50,0.25432,0.96619,0.042346,-35.759,0.96711,-0.25431,-0.0057645,40.158,0.0051995,0.042419,-0.99909,250.22
> view matrix models
> #50,-0.77557,0.61543,-0.14049,175.22,-0.61485,-0.78688,-0.052719,333.03,-0.143,0.045495,0.98868,11.993
> color #50 #FCEDFF
> select subtract #50
Nothing selected
> hide #!49 models
> hide #!50 models
> show #!50 models
> volume #50 level 1.954
> open "/Users/peiyuyu/Documents/Goethe
> University/2026/5_Manuscripts/1_AcrF/Figure/Figure_cryoEM_workflow/SA-
> F_B1/cryosparc_P4_J305_003_volume_map_sharp.mrc"
Opened cryosparc_P4_J305_003_volume_map_sharp.mrc as #51, grid size 80,80,80,
pixel 3.32, shown at level 0.569, step 1, values float32
> hide #!50 models
> select add #51
2 models selected
> view matrix models
> #51,-0.9391,-0.22026,0.26379,268.58,-0.1463,0.9508,0.27307,-8.1662,-0.31096,0.21785,-0.92512,264.01
> volume flip #51
Opened cryosparc_P4_J305_003_volume_map_sharp.mrc z flip as #52, grid size
80,80,80, pixel 3.32, shown at step 1, values float32
> close #51
> rename #52 CS_P4_J305_sharp_flip id #51
> save CS_P4_J305_sharp_flip.mrc #51
> color #51 #FCEDFF
> show #!50 models
> select add #51
2 models selected
> view matrix models
> #51,0.99548,-0.078687,0.053201,5.068,0.073705,0.99322,0.089881,-20.578,-0.059913,-0.085553,0.99453,15.159
> fitmap #51 inMap #50
Fit map CS_P4_J305_sharp_flip.mrc in map CS_P4_J304_sharp_flip.mrc using 5112
points
correlation = 0.7769, correlation about mean = 0.3462, overlap = 4325
steps = 92, shift = 6.39, angle = 27.1 degrees
Position of CS_P4_J305_sharp_flip.mrc (#51) relative to
CS_P4_J304_sharp_flip.mrc (#50) coordinates:
Matrix rotation and translation
-0.48191930 -0.86211205 -0.15657780 337.25915719
0.86174903 -0.49867166 0.09335524 70.84333299
-0.15856359 -0.08994107 0.98324371 36.25215794
Axis -0.10573285 0.00114549 0.99439391
Axis point 149.95468753 133.49561797 0.00000000
Rotation angle (degrees) 119.91228641
Shift along axis 0.47070508
> select subtract #51
Nothing selected
> hide #!50 models
> hide #!51 models
> open "/Users/peiyuyu/Documents/Goethe
> University/2026/5_Manuscripts/1_AcrF/Figure/Figure_cryoEM_workflow/SA-
> F_B1/cryosparc_P4_J306_002_volume_map_sharp.mrc"
Opened cryosparc_P4_J306_002_volume_map_sharp.mrc as #52, grid size 80,80,80,
pixel 3.32, shown at level 0.89, step 1, values float32
> select add #52
2 models selected
> view matrix models
> #52,0.64736,0.71151,-0.27328,-15.881,0.69766,-0.69754,-0.16345,155.65,-0.30692,-0.084848,-0.94795,287.9
> volume flip #52
Opened cryosparc_P4_J306_002_volume_map_sharp.mrc z flip as #53, grid size
80,80,80, pixel 3.32, shown at step 1, values float32
> close #52
> select add #53
2 models selected
> show #!51 models
> view matrix models
> #53,-0.54969,0.82414,-0.13652,114.28,-0.83504,-0.54665,0.062273,314.18,-0.023306,0.14823,0.98868,-59.023
> ui mousemode right "translate selected models"
> view matrix models
> #53,-0.54969,0.82414,-0.13652,112.71,-0.83504,-0.54665,0.062273,313.11,-0.023306,0.14823,0.98868,-22.329
> fitmap #53 inMap #51
Fit map cryosparc_P4_J306_002_volume_map_sharp.mrc z flip in map
CS_P4_J305_sharp_flip.mrc using 5118 points
correlation = 0.8696, correlation about mean = 0.2872, overlap = 4918
steps = 72, shift = 6.47, angle = 20.6 degrees
Position of cryosparc_P4_J306_002_volume_map_sharp.mrc z flip (#53) relative
to CS_P4_J305_sharp_flip.mrc (#51) coordinates:
Matrix rotation and translation
-0.48951817 0.85648171 -0.16374082 109.67271887
-0.85572951 -0.50794021 -0.09860917 331.28158536
-0.16762749 0.09184687 0.98156263 12.77250071
Axis 0.11055185 0.00225605 -0.99386780
Axis point 150.74970822 134.14339059 0.00000000
Rotation angle (degrees) 120.52723515
Shift along axis 0.17773418
> select subtract #53
Nothing selected
> hide #!51 models
> rename #53 CS_P4_J306_sharp_flip id #52
> save CS_P4_J306_sharp_flip.mrc #52
> open "/Users/peiyuyu/Documents/Goethe
> University/2026/5_Manuscripts/1_AcrF/Figure/Figure_cryoEM_workflow/SA-
> F_B1/cryosparc_P4_J307_002_volume_map_sharp.mrc"
Opened cryosparc_P4_J307_002_volume_map_sharp.mrc as #53, grid size 80,80,80,
pixel 3.32, shown at level 0.417, step 1, values float32
> hide #!52 models
> select add #53
2 models selected
> ui mousemode right "rotate selected models"
> view matrix models
> #53,-0.97802,-0.15767,0.13647,275.15,-0.15325,0.98728,0.042392,17.478,-0.14141,0.020547,-0.98974,260.92
> volume flip #53
Opened cryosparc_P4_J307_002_volume_map_sharp.mrc z flip as #54, grid size
80,80,80, pixel 3.32, shown at step 1, values float32
> close #53
> select add #54
2 models selected
> show #!51 models
> view matrix models
> #54,-0.83454,0.54308,0.09272,165.84,-0.53719,-0.83947,0.081916,314.21,0.12232,0.018554,0.99232,-51.868
> ui mousemode right "translate selected models"
> view matrix models
> #54,-0.83454,0.54308,0.09272,166.01,-0.53719,-0.83947,0.081916,312.6,0.12232,0.018554,0.99232,-21.112
> fitmap #54 inMap #51
Fit map cryosparc_P4_J307_002_volume_map_sharp.mrc z flip in map
CS_P4_J305_sharp_flip.mrc using 5118 points
correlation = 0.9157, correlation about mean = 0.3086, overlap = 1346
steps = 92, shift = 8.03, angle = 16.7 degrees
Position of cryosparc_P4_J307_002_volume_map_sharp.mrc z flip (#54) relative
to CS_P4_J305_sharp_flip.mrc (#51) coordinates:
Matrix rotation and translation
-0.49196091 0.85478647 -0.16527113 110.59805296
-0.85414546 -0.51062861 -0.09845785 330.98838770
-0.16855261 0.09272817 0.98132130 13.11747971
Axis 0.11118076 0.00190829 -0.99379837
Axis point 150.81392912 133.83273648 0.00000000
Rotation angle (degrees) 120.70607669
Shift along axis -0.10813355
> select subtract #54
Nothing selected
> rename #54 CS_P4_J307_sharp_flip id #53
> save CS_P4_J307_sharp_flip.mrc #53
> open "/Users/peiyuyu/Documents/Goethe
> University/2026/5_Manuscripts/1_AcrF/Figure/Figure_cryoEM_workflow/SA-
> F_B1/cryosparc_P4_J308_003_volume_map_sharp.mrc"
Opened cryosparc_P4_J308_003_volume_map_sharp.mrc as #54, grid size 80,80,80,
pixel 3.32, shown at level 0.487, step 1, values float32
> hide #!51 models
> hide #!53 models
> select add #54
2 models selected
> view matrix models #54,1,0,0,-2.2085,0,1,0,5.106,0,0,1,17.329
> ui mousemode right "rotate selected models"
> view matrix models
> #54,-0.97907,-0.098429,0.17813,263.99,-0.078474,0.99017,0.11581,3.2524,-0.18778,0.099408,-0.97717,270.12
> volume flip #54
Opened cryosparc_P4_J308_003_volume_map_sharp.mrc z flip as #55, grid size
80,80,80, pixel 3.32, shown at step 1, values float32
> close #54
> select add #55
2 models selected
> show #!53 models
> view matrix models
> #55,-0.032009,-0.99944,0.0094019,279.03,0.99475,-0.030941,0.097574,-4.0943,-0.097229,0.012476,0.99518,-8.8511
> ui mousemode right "translate selected models"
> view matrix models
> #55,-0.032009,-0.99944,0.0094019,275.5,0.99475,-0.030941,0.097574,4.3503,-0.097229,0.012476,0.99518,10.879
> fitmap #55 inMap #53
Fit map cryosparc_P4_J308_003_volume_map_sharp.mrc z flip in map
CS_P4_J307_sharp_flip.mrc using 5116 points
correlation = 0.9635, correlation about mean = 0.3444, overlap = 1309
steps = 80, shift = 11, angle = 8.02 degrees
Position of cryosparc_P4_J308_003_volume_map_sharp.mrc z flip (#55) relative
to CS_P4_J307_sharp_flip.mrc (#53) coordinates:
Matrix rotation and translation
-0.47079667 0.87008681 -0.14594324 102.09450866
-0.87101131 -0.48471046 -0.07996914 326.37268834
-0.14032031 0.08946901 0.98605553 9.07494900
Axis 0.09685875 -0.00321433 -0.99529295
Axis point 148.07793015 132.95152312 0.00000000
Rotation angle (degrees) 118.99450917
Shift along axis -0.19255539
> select subtract #55
Nothing selected
> hide #!53 models
> color #55 #fcedffff models
> rename #55 CS_P4_J308_sharp_flip id #54
> save CS_P4_J308_sharp_flip.mrc #54
> open "/Users/peiyuyu/Documents/Goethe
> University/2026/5_Manuscripts/1_AcrF/Figure/Figure_cryoEM_workflow/SA-
> F_B1/cryosparc_P4_J309_002_volume_map_sharp.mrc"
Opened cryosparc_P4_J309_002_volume_map_sharp.mrc as #55, grid size 80,80,80,
pixel 3.32, shown at level 0.427, step 1, values float32
> hide #!54 models
> select add #55
2 models selected
> show #!54 models
> ui mousemode right "rotate selected models"
> view matrix models
> #55,0.72482,-0.68817,-0.032661,133.9,-0.68882,-0.72298,-0.053201,331.74,0.012998,0.061058,-0.99805,234.36
> hide #!54 models
> view matrix models
> #55,-0.98503,0.14104,0.099091,242.98,0.14443,0.98912,0.027904,-21.89,-0.094078,0.041798,-0.99469,251.29
> volume flip #55
Opened cryosparc_P4_J309_002_volume_map_sharp.mrc z flip as #56, grid size
80,80,80, pixel 3.32, shown at step 1, values float32
> close #55
> select add #56
2 models selected
> show #!54 models
> view matrix models
> #56,-0.63923,0.76709,0.054278,117.91,-0.76391,-0.64153,0.069883,315.42,0.088428,0.0032076,0.99608,-48.086
> ui mousemode right "translate selected models"
> view matrix models
> #56,-0.63923,0.76709,0.054278,118.17,-0.76391,-0.64153,0.069883,313.6,0.088428,0.0032076,0.99608,-18.356
> fitmap #56 inMap #54
Fit map cryosparc_P4_J309_002_volume_map_sharp.mrc z flip in map
CS_P4_J308_sharp_flip.mrc using 5111 points
correlation = 0.9585, correlation about mean = 0.3571, overlap = 1283
steps = 104, shift = 10.8, angle = 17.1 degrees
Position of cryosparc_P4_J309_002_volume_map_sharp.mrc z flip (#56) relative
to CS_P4_J308_sharp_flip.mrc (#54) coordinates:
Matrix rotation and translation
-0.48831434 -0.86312376 -0.12871093 333.56502617
0.86459679 -0.49852007 0.06285020 75.43057430
-0.11841248 -0.08059241 0.98968852 28.18171624
Axis -0.08273806 -0.00594017 0.99655362
Axis point 146.06051902 134.60897835 0.00000000
Rotation angle (degrees) 119.90563147
Shift along axis 0.03799683
> select subtract #56
Nothing selected
> hide #!54 models
> hide #!56 models
> show #!56 models
> color #56 #fcedffff models
> rename #56 CS_P4_J309_sharp_flip id #55
> save CS_P4_J309_sharp_flip.mrc #55
> open "/Users/peiyuyu/Documents/Goethe
> University/2026/5_Manuscripts/1_AcrF/Figure/Figure_cryoEM_workflow/SA-
> F_B1/cryosparc_P4_J310_003_volume_map_sharp.mrc"
Opened cryosparc_P4_J310_003_volume_map_sharp.mrc as #56, grid size 80,80,80,
pixel 3.32, shown at level 0.683, step 1, values float32
> volume flip #56
Opened cryosparc_P4_J310_003_volume_map_sharp.mrc z flip as #57, grid size
80,80,80, pixel 3.32, shown at step 1, values float32
> close #56
> fitmap #56 inMap #52
No atoms or maps for #56
> fitmap #57 inMap #52
Fit map cryosparc_P4_J310_003_volume_map_sharp.mrc z flip in map
CS_P4_J306_sharp_flip.mrc using 5115 points
correlation = 0.769, correlation about mean = 0.3701, overlap = 4288
steps = 88, shift = 5.42, angle = 22.7 degrees
Position of cryosparc_P4_J310_003_volume_map_sharp.mrc z flip (#57) relative
to CS_P4_J306_sharp_flip.mrc (#52) coordinates:
Matrix rotation and translation
-0.48166864 -0.85899522 -0.17355843 339.66736714
0.86194402 -0.50013127 0.08319372 72.72621082
-0.15826501 -0.10952585 0.98130335 38.95738038
Axis -0.11128512 -0.00883113 0.99374928
Axis point 150.89500052 135.18260516 0.00000000
Rotation angle (degrees) 120.01642768
Shift along axis 0.27169032
> hide #!55 models
> show #!52 models
> color #57 #fcedffff models
> hide #!52 models
> save #57 CS_P4_J310_sharp_flip id #56
Cannot determine format for '#57'
> rename #57 CS_P4_J310_sharp_flip id #56
> save CS_P4_J310_sharp_flip.mrc #56
> open "/Users/peiyuyu/Documents/Goethe
> University/2026/5_Manuscripts/1_AcrF/Figure/Figure_cryoEM_workflow/SA-
> F_B1/cryosparc_P4_J311_004_volume_map_sharp.mrc"
Opened cryosparc_P4_J311_004_volume_map_sharp.mrc as #57, grid size 80,80,80,
pixel 3.32, shown at level 0.525, step 1, values float32
> volume flip #57
Opened cryosparc_P4_J311_004_volume_map_sharp.mrc z flip as #58, grid size
80,80,80, pixel 3.32, shown at step 1, values float32
> close #57
> color #58 #fcedffff models
> rename #58 CS_P4_J311_sharp_flip id #57
> save CS_P4_J311_sharp_flip.mrc #57
> save "/Users/peiyuyu/Documents/Goethe
> University/2026/5_Manuscripts/1_AcrF/Figure/Figure_cryoEM_workflow/SA-
> F_B1/20260506_SA-F_B1_workflow.cxs"
> view P1
> hide #!57 models
> hide #!56 models
> show #!47 models
> hide #!47 models
> show #!48 models
> save CS_P4_J302_NU_sharp.png supersample 3 transparentBackground true
> hide #!48 models
> show #!49 models
> save CS_P4_J303_NU_sharp.png supersample 3 transparentBackground true
> hide #!49 models
> show #!50 models
> save CS_P4_J304_NU_sharp.png supersample 3 transparentBackground true
> hide #!50 models
> show #!51 models
> save CS_P4_J305_NU_sharp.png supersample 3 transparentBackground true
> hide #!51 models
> show #!52 models
> volume #52 level 1.034
> save CS_P4_J306_NU_sharp.png supersample 3 transparentBackground true
> hide #!52 models
> show #!53 models
> color #53 #fcedffff models
> save CS_P4_J307_NU_sharp.png supersample 3 transparentBackground true
> hide #!53 models
> show #!54 models
> save CS_P4_J308_NU_sharp.png supersample 3 transparentBackground true
> hide #!54 models
> show #!55 models
> save CS_P4_J309_NU_sharp.png supersample 3 transparentBackground true
> hide #!55 models
> show #!56 models
> volume #56 level 0.7192
> save CS_P4_J310_NU_sharp.png supersample 3 transparentBackground true
> hide #!56 models
> show #!57 models
> save CS_P4_J311_NU_sharp.png supersample 3 transparentBackground true
> save "/Users/peiyuyu/Documents/Goethe
> University/2026/5_Manuscripts/1_AcrF/Figure/Figure_cryoEM_workflow/SA-
> F_B1/20260506_SA-F_B1_workflow.cxs"
——— End of log from Wed May 6 15:19:28 2026 ———
> view name session-start
opened ChimeraX session
> open "/Users/peiyuyu/Documents/Goethe
> University/2026/5_Manuscripts/1_AcrF/Figure/Figure_cryoEM_workflow/SA-
> F_B1/cryosparc_P4_J323_006_volume_map_sharp.mrc"
Opened cryosparc_P4_J323_006_volume_map_sharp.mrc as #58, grid size
320,320,320, pixel 0.831, shown at level 0.0345, step 2, values float32
> open "/Users/peiyuyu/Documents/Goethe
> University/2026/5_Manuscripts/1_AcrF/Figure/Figure_cryoEM_workflow/SA-
> F_B1/cryosparc_P4_J324_map_locres.mrc"
Opened cryosparc_P4_J324_map_locres.mrc as #59, grid size 320,320,320, pixel
0.831, shown at level 8.89, step 2, values float32
> hide #!57 models
> hide #!58 models
> show #!58 models
> hide #!59 models
> show #!59 models
> volume flip #58
Opened cryosparc_P4_J323_006_volume_map_sharp.mrc z flip as #60, grid size
320,320,320, pixel 0.831, shown at step 1, values float32
> volume flip #59
Opened cryosparc_P4_J324_map_locres.mrc z flip as #61, grid size 320,320,320,
pixel 0.831, shown at step 1, values float32
> close #58
> close #59
> rename #60 CS_P4_J323_sharp_flip #58
Expected a keyword
> rename #60 CS_P4_J323_sharp_flip id #58
> save CS_P4_J323_sharp_flip.mrc #58
> rename CS_P4_J324_map_locres_flip id #59
Missing or invalid "models" argument: invalid models specifier
> rename #61 CS_P4_J324_map_locres_flip id #59
> save CS_P4_J324_map_locres_flip.mrc #59
> hide #!58 models
> show #!58 models
> hide #!59 models
> volume #59 level 9.377
> hide #!59 models
> volume #58 level 0.05889
> show #!59 models
> volume #59 level 3.695
> save "/Users/peiyuyu/Documents/Goethe
> University/2026/5_Manuscripts/1_AcrF/Figure/Figure_cryoEM_workflow/SA-
> F_B1/20260507_SA-F_B1_workflow.cxs"
> ui tool show "Surface Color"
> hide #!58 models
> show #!58 models
> hide #!59 models
> color radial #58.1 palette #ffb17f:#4979d3:#99bfe5:#fff6c0:#f4fff3
> color sample #58.1 map #59 palette
> 166.9,#ffb17f:198,#4979d3:229.1,#99bfe5:260.3,#fff6c0:291.4,#f4fff3
> outsideColor #808080
Map values for surface "surface": minimum 2.944, mean 3.552, maximum 44.59
> color sample #58.1 map #59 palette
> 2,#ffb17f:3,#4979d3:4,#99bfe5:5,#fff6c0:6,#f4fff3 outsideColor #808080
Map values for surface "surface": minimum 2.944, mean 3.552, maximum 44.59
> show #!59 models
> hide #!58 models
> hide #!59 models
> show #!59 models
> show #!58 models
> hide #!59 models
> show #!59 models
> hide #!59 models
> volume #58 level 0.0634
> volume #58 level 0.04264
> volume #58 level 0.07693
> volume #58 level 0.06069
> volume #58 level 0.07152
> volume #58 level 0.05889
> volume #58 level 0.05167
> volume #58 level 0.06159
> key #ffb17f:2 #4979d3:3 #99bfe5:4 #fff6c0:5 #f4fff3:6 showTool true
> ui mousemode right "color key"
> key pos 0.82265,0.196044 size 0.0692066,0.202924
> ui mousemode right translate
> ui mousemode right "color key"
> key pos 0.802943,0.231339 size 0.039822,0.144632
> key #ffb17f:2 #4979d3:3 #99bfe5:4 #fff6c0:5 #f4fff3:6 showTool true
> key pos 0.836801,0.165731 size 0.0516869,0.238605
> show #41 models
> key pos 0.820538,0.190547 size 0.0458442,0.242723
> save CS_P4_J323_sharp_flip.png supersample 3 transparentBackground true
> save "/Users/peiyuyu/Documents/Goethe
> University/2026/5_Manuscripts/1_AcrF/Figure/Figure_cryoEM_workflow/SA-
> F_B1/20260507_SA-F_B1_workflow1.cxs"
> color sample #58.1 map #59 palette
> 2,#ffb17f:3,#169bd3:4,#99bfe5:5,#fff6c0:6,#f4fff3 outsideColor #808080
Map values for surface "surface": minimum 2.944, mean 3.532, maximum 44.61
> color sample #58.1 map #59 palette
> 2,#ffb17f:3,#46c7f6:4,#99bfe5:5,#fff6c0:6,#f4fff3 outsideColor #808080
Map values for surface "surface": minimum 2.944, mean 3.532, maximum 44.61
> color sample #58.1 map #59 palette
> 2,#ffb17f:3,#11b6e1:4,#99bfe5:5,#fff6c0:6,#f4fff3 outsideColor #808080
Map values for surface "surface": minimum 2.944, mean 3.532, maximum 44.61
> key #ffb17f:2 #11b6e1:3 #99bfe5:4 #fff6c0:5 #f4fff3:6 showTool true
> ui mousemode right "color key"
> color sample #58.1 map #59 palette
> 2,#ffb17f:3,#11b6e1:4,#99bfe5:5,#fff6c0:6,#ffb17f outsideColor #808080
Map values for surface "surface": minimum 2.944, mean 3.532, maximum 44.61
> key #ffb17f:2 #11b6e1:3 #99bfe5:4 #fff6c0:5 #ffb17f:6 showTool true
> color sample #58.1 map #59 palette
> 2,#13097f:3,#11b6e1:4,#99bfe5:5,#fff6c0:6,#ffb17f outsideColor #808080
Map values for surface "surface": minimum 2.944, mean 3.532, maximum 44.61
> key #13097f:2 #11b6e1:3 #99bfe5:4 #fff6c0:5 #ffb17f:6 showTool true
> save CS_P4_J323_sharp_flip2.png supersample 3 transparentBackground true
> save "/Users/peiyuyu/Documents/Goethe
> University/2026/5_Manuscripts/1_AcrF/Figure/Figure_cryoEM_workflow/SA-
> F_B1/20260507_SA-F_B1_workflow2.cxs"
> open "/Users/peiyuyu/Documents/Goethe
> University/2026/5_Manuscripts/1_AcrF/Figure/Figure_cryoEM_workflow/SA-
> F_B1/cryosparc_P4_J329_005_volume_map_sharp.mrc"
Opened cryosparc_P4_J329_005_volume_map_sharp.mrc as #60, grid size
320,320,320, pixel 0.831, shown at level 0.0384, step 2, values float32
> open "/Users/peiyuyu/Documents/Goethe
> University/2026/5_Manuscripts/1_AcrF/Figure/Figure_cryoEM_workflow/SA-
> F_B1/cryosparc_P4_J330_map_locres.mrc"
Opened cryosparc_P4_J330_map_locres.mrc as #61, grid size 320,320,320, pixel
0.831, shown at level 9.71, step 2, values float32
> hide #!58 models
> hide #!60 models
> show #!60 models
> hide #!61 models
> volume flip #60
Opened cryosparc_P4_J329_005_volume_map_sharp.mrc z flip as #62, grid size
320,320,320, pixel 0.831, shown at step 1, values float32
> volume flip #61
Opened cryosparc_P4_J330_map_locres.mrc z flip as #63, grid size 320,320,320,
pixel 0.831, shown at step 1, values float32
> close #60
> close #61
> rename #62 CS_P4_J329_sharp_flip id #60
> save CS_P4_J329_sharp_flip.mrc #60
> rename #63 rename
> rename #63 CS_P4_J330_locres_flip id #61
> save CS_P4_J330_locres_flip.mrc #61
> save "/Users/peiyuyu/Documents/Goethe
> University/2026/5_Manuscripts/1_AcrF/Figure/Figure_cryoEM_workflow/SA-
> F_B1/20260507_SA-F_B1_workflow3.cxs"
> hide #!61 models
> color sample #58.1 map #59 palette
> 2,#13097f:3,#11b6e1:4,#99bfe5:5,#fff6c0:6,#ffb17f outsideColor #808080
Map values for surface "surface": minimum 2.944, mean 3.532, maximum 44.61
> color sample #60.1 map #61 palette
> 2,#13097f:3,#11b6e1:4,#99bfe5:5,#fff6c0:6,#ffb17f outsideColor #808080
Map values for surface "surface": minimum 0, mean 4.466, maximum 46.71
> volume #60 level 0.06443
> key #13097f:2 #11b6e1:3 #99bfe5:4 #fff6c0:5 #ffb17f:6 showTool true
> ui mousemode right "color key"
> volume #60 level 0.05663
> save CS_P4_J329_locres_sharp.png supersample 3 transparentBackground true
> save "/Users/peiyuyu/Documents/Goethe
> University/2026/5_Manuscripts/1_AcrF/Figure/Figure_cryoEM_workflow/SA-
> F_B1/20260507_SA-F_B1_workflow3.cxs"
——— End of log from Thu May 7 15:58:17 2026 ———
> view name session-start
opened ChimeraX session
> open "/Users/peiyuyu/Documents/Goethe
> University/2026/5_Manuscripts/1_AcrF/Figure/Figure_cryoEM_workflow/SA-
> F_B1/cryosparc_P4_J300_005_volume_map_sharp.mrc"
Opened cryosparc_P4_J300_005_volume_map_sharp.mrc as #62, grid size
320,320,320, pixel 0.831, shown at level 0.0375, step 2, values float32
> open "/Users/peiyuyu/Documents/Goethe
> University/2026/5_Manuscripts/1_AcrF/Figure/Figure_cryoEM_workflow/SA-
> F_B1/cryosparc_P4_J301_map_locres.mrc"
Opened cryosparc_P4_J301_map_locres.mrc as #63, grid size 320,320,320, pixel
0.831, shown at level 9.68, step 2, values float32
> hide #!60 models
> hide #!63 models
> volume flip #62
Opened cryosparc_P4_J300_005_volume_map_sharp.mrc z flip as #64, grid size
320,320,320, pixel 0.831, shown at step 1, values float32
> volume flip #63
Opened cryosparc_P4_J301_map_locres.mrc z flip as #65, grid size 320,320,320,
pixel 0.831, shown at step 1, values float32
> close #62
> close #63
> rename #64 CS_P4_J300_sharp_flip id #62
> save CS_P4_J300_sharp_flip.mrc #62
> rename #65 CS_P4_J301_locres_flip id #63
> save CS_P4_J301_locres_flip.mrc #63
> show #!46 models
> show #!47 models
> hide #!47 models
> hide #!46 models
> hide #!63 models
> show #!63 models
> volume #63 level 3.952
> hide #!63 models
> volume #62 level 0.0392
> ui tool show "Surface Color"
> color sample #62.1 map #63 palette #13097f:#11b6e1:#99bfe5:#fff6c0:#ffb17f
> outsideColor #808080
Map values for surface "surface": minimum 0, mean 4.383, maximum 43.37
> color sample #62.1 map #63 palette
> 2,#13097f:3,#11b6e1:4,#99bfe5:5,#fff6c0:6,#ffb17f outsideColor #808080
Map values for surface "surface": minimum 0, mean 4.383, maximum 43.37
> show #!63 models
> hide #!63 models
> hide #!62 models
> show #!62 models
> volume #62 level 0.06614
> volume #62 level 0.05745
> save CS_P4_J300_locres_sharp.png supersample 3 transparentBackground true
> save "/Users/peiyuyu/Documents/Goethe
> University/2026/5_Manuscripts/1_AcrF/Figure/Figure_cryoEM_workflow/SA-
> F_B1/20260507_SA-F_B1_workflow4.cxs"
> open /Users/peiyuyu/Downloads/cryosparc_P7_J110_004_volume_map_sharp.mrc
Opened cryosparc_P7_J110_004_volume_map_sharp.mrc as #64, grid size
160,160,160, pixel 1.66, shown at level 0.28, step 1, values float32
> hide #!62 models
> close #64
> save "/Users/peiyuyu/Documents/Goethe
> University/2026/5_Manuscripts/1_AcrF/Figure/Figure_cryoEM_workflow/SA-
> F_B1/20260507_SA-F_B1_workflow5.cxs"
——— End of log from Thu May 7 21:11:39 2026 ———
> view name session-start
opened ChimeraX session
Failed to send bug report. Bug report server www.rbvi.ucsf.edu is unavailable
[Repeated 1 time(s)]
OpenGL version: 4.1 Metal - 89.3
OpenGL renderer: Apple M4 Pro
OpenGL vendor: Apple
Python: 3.11.9
Locale: UTF-8
Qt version: PyQt6 6.9.1, Qt 6.9.0
Qt runtime version: 6.9.2
Qt platform: cocoa
Hardware:
Hardware Overview:
Model Name: MacBook Pro
Model Identifier: Mac16,8
Model Number: MX2F3D/A
Chip: Apple M4 Pro
Total Number of Cores: 14 (10 performance and 4 efficiency)
Memory: 24 GB
System Firmware Version: 11881.81.4
OS Loader Version: 11881.81.4
Software:
System Software Overview:
System Version: macOS 15.3.1 (24D70)
Kernel Version: Darwin 24.3.0
Time since boot: 29天16小時42分鐘
Graphics/Displays:
Apple M4 Pro:
Chipset Model: Apple M4 Pro
Type: GPU
Bus: Built-In
Total Number of Cores: 20
Vendor: Apple (0x106b)
Metal Support: Metal 3
Displays:
Color LCD:
Display Type: Built-in Liquid Retina XDR Display
Resolution: 3024 x 1964 Retina
Main Display: Yes
Mirror: Off
Online: Yes
Automatically Adjust Brightness: Yes
Connection Type: Internal
LEN T22i-10:
Resolution: 1920 x 1080 (1080p FHD - Full High Definition)
UI Looks like: 1920 x 1080 @ 60.00Hz
Mirror: Off
Online: Yes
Rotation: Supported
Installed Packages:
aiohappyeyeballs: 2.6.1
aiohttp: 3.13.1
aiosignal: 1.4.0
alabaster: 1.0.0
annotated-types: 0.7.0
anyio: 4.12.0
appdirs: 1.4.4
appnope: 0.1.4
asttokens: 3.0.1
attrs: 25.4.0
babel: 2.17.0
beautifulsoup4: 4.13.5
blockdiag: 3.0.0
blosc2: 3.12.2
build: 1.3.0
certifi: 2025.7.14
cftime: 1.6.5
charset-normalizer: 3.4.4
ChimeraX-AddCharge: 1.5.20
ChimeraX-AddH: 2.2.8
ChimeraX-AlignmentAlgorithms: 2.0.2
ChimeraX-AlignmentHdrs: 3.6.1
ChimeraX-AlignmentMatrices: 2.1
ChimeraX-Alignments: 3.1.1
ChimeraX-AlphaFold: 1.0.1
ChimeraX-AltlocExplorer: 1.2
ChimeraX-AmberInfo: 1.0
ChimeraX-Animations: 1.0
ChimeraX-Aniso: 1.3.2
ChimeraX-Arrays: 1.1
ChimeraX-Atomic: 1.61.4
ChimeraX-AtomicLibrary: 14.2.1
ChimeraX-AtomSearch: 2.0.1
ChimeraX-AxesPlanes: 2.4
ChimeraX-BasicActions: 1.1.3
ChimeraX-BILD: 1.0
ChimeraX-BlastProtein: 3.0.0
ChimeraX-Boltz: 1.1
ChimeraX-BondRot: 2.0.4
ChimeraX-BugReporter: 1.0.2
ChimeraX-BuildStructure: 2.13.1
ChimeraX-Bumps: 1.0
ChimeraX-BundleBuilder: 1.6.0
ChimeraX-ButtonPanel: 1.0.1
ChimeraX-CageBuilder: 1.0.1
ChimeraX-CellPack: 1.0
ChimeraX-Centroids: 1.4
ChimeraX-ChangeChains: 1.1
ChimeraX-CheckWaters: 1.5
ChimeraX-ChemGroup: 2.0.2
ChimeraX-Clashes: 2.3
ChimeraX-ColorActions: 1.0.5
ChimeraX-ColorGlobe: 1.0
ChimeraX-ColorKey: 1.5.8
ChimeraX-CommandLine: 1.3.0
ChimeraX-ConnectStructure: 2.0.1
ChimeraX-Contacts: 1.0.1
ChimeraX-Core: 1.11
ChimeraX-CoreFormats: 1.2
ChimeraX-coulombic: 1.4.5
ChimeraX-Crosslinks: 1.0
ChimeraX-Crystal: 1.0
ChimeraX-CrystalContacts: 1.0.1
ChimeraX-DataFormats: 1.2.4
ChimeraX-Dicom: 1.2.7
ChimeraX-DistMonitor: 1.4.2
ChimeraX-DockPrep: 1.1.4
ChimeraX-Dssp: 2.0
ChimeraX-EMDB-SFF: 1.0
ChimeraX-ESMFold: 1.0
ChimeraX-FileHistory: 1.0.1
ChimeraX-FunctionKey: 1.0.1
ChimeraX-Geometry: 1.3
ChimeraX-gltf: 1.0
ChimeraX-Graphics: 1.4.1
ChimeraX-Hbonds: 2.5.3
ChimeraX-Help: 1.3
ChimeraX-HKCage: 1.3
ChimeraX-IHM: 1.1
ChimeraX-ImageFormats: 1.2
ChimeraX-IMOD: 1.0
ChimeraX-IO: 1.0.4
ChimeraX-ItemsInspection: 1.0.1
ChimeraX-IUPAC: 1.0
ChimeraX-KVFinder: 1.7.3
ChimeraX-Label: 1.2
ChimeraX-ListInfo: 1.2.2
ChimeraX-Log: 1.2.2
ChimeraX-LookingGlass: 1.1
ChimeraX-Maestro: 1.9.2
ChimeraX-Map: 1.3
ChimeraX-MapData: 2.0
ChimeraX-MapEraser: 1.0.1
ChimeraX-MapFilter: 2.0.1
ChimeraX-MapFit: 2.0
ChimeraX-MapSeries: 2.1.1
ChimeraX-Markers: 1.0.1
ChimeraX-Mask: 1.0.2
ChimeraX-MatchMaker: 2.2.2
ChimeraX-MCopy: 1.0
ChimeraX-MCPServer: 0.1.0
ChimeraX-MDcrds: 2.17.2
ChimeraX-MedicalToolbar: 1.1
ChimeraX-Meeting: 1.0.1
ChimeraX-Minimize: 1.3.2
ChimeraX-MLP: 1.1.1
ChimeraX-mmCIF: 2.16
ChimeraX-MMTF: 2.2
ChimeraX-ModelArchive: 1.0
ChimeraX-Modeller: 1.5.22
ChimeraX-ModelPanel: 1.6
ChimeraX-ModelSeries: 1.0.1
ChimeraX-Mol2: 2.0.3
ChimeraX-Mole: 1.0
ChimeraX-Morph: 1.0.2
ChimeraX-MouseModes: 1.2
ChimeraX-Movie: 1.0.1
ChimeraX-MutationScores: 1.0
ChimeraX-Neuron: 1.0
ChimeraX-Nifti: 1.2
ChimeraX-NMRSTAR: 1.0.2
ChimeraX-NRRD: 1.2
ChimeraX-Nucleotides: 2.0.3
ChimeraX-OpenCommand: 1.15.2
ChimeraX-OrthoPick: 1.0.1
ChimeraX-PDB: 2.7.11
ChimeraX-PDBBio: 1.0.1
ChimeraX-PDBLibrary: 1.0.5
ChimeraX-PDBMatrices: 1.0
ChimeraX-PickBlobs: 1.0.1
ChimeraX-Positions: 1.0
ChimeraX-PresetMgr: 1.1.4
ChimeraX-ProfileGrids: 1.4.2
ChimeraX-PubChem: 2.2
ChimeraX-ReadPbonds: 1.0.1
ChimeraX-Registration: 1.1.2
ChimeraX-RemoteControl: 1.0
ChimeraX-RenderByAttr: 1.6.5
ChimeraX-RenumberResidues: 1.1
ChimeraX-ResidueFit: 1.0.1
ChimeraX-RestServer: 1.3.3
ChimeraX-RNALayout: 1.0
ChimeraX-RotamerLibMgr: 4.0
ChimeraX-RotamerLibsDunbrack: 2.0
ChimeraX-RotamerLibsDynameomics: 2.0
ChimeraX-RotamerLibsRichardson: 2.0
ChimeraX-SaveCommand: 1.5.2
ChimeraX-Scenes: 0.3.1
ChimeraX-SchemeMgr: 1.0
ChimeraX-SDF: 2.0.3
ChimeraX-Segger: 1.0
ChimeraX-Segment: 1.0.1
ChimeraX-Segmentations: 3.5.10
ChimeraX-SelInspector: 1.0
ChimeraX-SeqView: 2.17.3
ChimeraX-Shape: 1.1
ChimeraX-Shell: 1.0.1
ChimeraX-Shortcuts: 1.2.1
ChimeraX-ShowSequences: 1.0.3
ChimeraX-SideView: 1.0.1
ChimeraX-SimilarStructures: 1.0.1
ChimeraX-Smiles: 2.1.2
ChimeraX-SmoothLines: 1.0
ChimeraX-SpaceNavigator: 1.0
ChimeraX-StdCommands: 1.19.1
ChimeraX-STL: 1.0.1
ChimeraX-Storm: 1.0
ChimeraX-StructMeasure: 1.2.1
ChimeraX-Struts: 1.0.1
ChimeraX-Surface: 1.0.1
ChimeraX-SwapAA: 2.0.1
ChimeraX-SwapRes: 2.5.2
ChimeraX-TapeMeasure: 1.0
ChimeraX-TaskManager: 1.0
ChimeraX-Test: 1.0
ChimeraX-Toolbar: 1.2.3
ChimeraX-ToolshedUtils: 1.2.4
ChimeraX-Topography: 1.0
ChimeraX-ToQuest: 1.0
ChimeraX-Tug: 1.0.1
ChimeraX-UI: 1.49.1
ChimeraX-Umap: 1.0
ChimeraX-uniprot: 2.3.2
ChimeraX-UnitCell: 1.0.1
ChimeraX-ViewDock: 1.5.2
ChimeraX-VIPERdb: 1.0
ChimeraX-Vive: 1.1
ChimeraX-VolumeMenu: 1.0.1
ChimeraX-vrml: 1.0
ChimeraX-VTK: 1.0
ChimeraX-WavefrontOBJ: 1.0
ChimeraX-WebCam: 1.0.2
ChimeraX-WebServices: 1.1.5
ChimeraX-Zone: 1.0.1
click: 8.3.1
colorama: 0.4.6
comm: 0.2.3
contourpy: 1.3.3
coverage: 7.13.0
cxservices: 1.2.3
cycler: 0.12.1
Cython: 3.1.4
debugpy: 1.8.19
decorator: 5.2.1
docutils: 0.21.2
executing: 2.2.1
filelock: 3.19.1
fonttools: 4.61.1
frozenlist: 1.8.0
funcparserlib: 2.0.0a0
glfw: 2.10.0
grako: 3.16.5
h11: 0.16.0
h5py: 3.15.1
html2text: 2025.4.15
httpcore: 1.0.9
httpx: 0.28.1
httpx-sse: 0.4.3
idna: 3.11
ihm: 2.2
imagecodecs: 2024.6.1
imagesize: 1.4.1
iniconfig: 2.3.0
ipykernel: 6.30.1
ipython: 9.5.0
ipython_pygments_lexers: 1.1.1
ipywidgets: 8.1.8
jedi: 0.19.2
Jinja2: 3.1.6
jsonschema: 4.25.1
jsonschema-specifications: 2025.9.1
jupyter_client: 8.6.3
jupyter_core: 5.9.1
jupyterlab_widgets: 3.0.16
kiwisolver: 1.4.9
line_profiler: 5.0.0
lxml: 6.0.2
lz4: 4.3.2
Markdown: 3.8.2
MarkupSafe: 3.0.3
matplotlib: 3.10.7
matplotlib-inline: 0.2.1
mcp: 1.18.0
msgpack: 1.1.1
multidict: 6.7.0
narwhals: 2.17.0
ndindex: 1.10.1
nest-asyncio: 1.6.0
netCDF4: 1.6.5
networkx: 3.3
nibabel: 5.2.0
nptyping: 2.5.0
numexpr: 2.14.1
numpy: 2.4.2
numpy: 1.26.4
OpenMM: 8.2.0
openvr: 1.26.701
packaging: 25.0
ParmEd: 4.2.2
parso: 0.8.5
pep517: 0.13.1
pexpect: 4.9.0
pickleshare: 0.7.5
pillow: 11.3.0
pip: 25.2
pkginfo: 1.12.1.2
platformdirs: 4.5.1
plotly: 6.5.2
pluggy: 1.6.0
prompt_toolkit: 3.0.52
propcache: 0.4.1
psutil: 7.0.0
ptyprocess: 0.7.0
pure_eval: 0.2.3
py-cpuinfo: 9.0.0
pybind11: 3.0.1
pycollada: 0.8
pydantic: 2.12.5
pydantic-settings: 2.12.0
pydantic_core: 2.41.5
pydicom: 2.4.4
Pygments: 2.18.0
pyKVFinder: 0.9.0
pynmrstar: 3.3.6
pynrrd: 1.0.0
PyOpenGL: 3.1.10
PyOpenGL-accelerate: 3.1.10
pyopenxr: 1.1.4501
pyparsing: 3.2.5
pyproject_hooks: 1.2.0
PyQt6-commercial: 6.9.1
PyQt6-Qt6: 6.9.2
PyQt6-WebEngine-commercial: 6.9.0
PyQt6-WebEngine-Qt6: 6.9.2
PyQt6_sip: 13.10.2
pytest: 9.0.2
pytest-cov: 7.0.0
python-dateutil: 2.9.0.post0
python-dotenv: 1.2.1
python-multipart: 0.0.21
pytz: 2025.2
pyzmq: 27.1.0
qtconsole: 5.7.0
QtPy: 2.4.3
qtshim: 1.2
RandomWords: 0.4.0
referencing: 0.37.0
requests: 2.32.5
roman-numerals: 4.0.0
roman-numerals-py: 4.0.0
rpds-py: 0.30.0
scipy: 1.14.0
setuptools: 80.9.0
sfftk-rw: 0.8.1
six: 1.17.0
snowballstemmer: 3.0.1
sortedcontainers: 2.4.0
soupsieve: 2.8
Sphinx: 8.2.3
sphinx-autodoc-typehints: 3.2.0
sphinxcontrib-applehelp: 2.0.0
sphinxcontrib-blockdiag: 3.0.0
sphinxcontrib-devhelp: 2.0.0
sphinxcontrib-htmlhelp: 2.1.0
sphinxcontrib-jsmath: 1.0.1
sphinxcontrib-qthelp: 2.0.0
sphinxcontrib-serializinghtml: 2.0.0
sse-starlette: 3.0.4
stack-data: 0.6.3
starlette: 0.50.0
superqt: 0.7.6
tables: 3.10.2
tcia_utils: 1.5.1
tifffile: 2025.3.13
tinyarray: 1.2.5
tomlkit: 0.14.0
tornado: 6.5.4
traitlets: 5.14.3
typing-inspection: 0.4.2
typing_extensions: 4.15.0
tzdata: 2025.3
urllib3: 2.6.2
uvicorn: 0.38.0
wcwidth: 0.2.14
webcolors: 24.11.1
wheel: 0.45.1
wheel-filename: 1.4.2
widgetsnbextension: 4.0.15
yarl: 1.22.0
Change History (1)
comment:1 by , 4 days ago
| Component: | Unassigned → Window Toolkit |
|---|---|
| Description: | modified (diff) |
| Owner: | set to |
| Platform: | → all |
| Project: | → ChimeraX |
| Status: | new → assigned |
| Summary: | ChimeraX bug report submission → Crash moving tool window, Mac multiple displays |
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