| 1718 | | > fitmap #1 inMap #2 |
| 1719 | | |
| 1720 | | Fit molecule 5zf6.pdb (#1) to map cryosparc_P1_J138_004_volume_map_sharp.mrc |
| 1721 | | (#2) using 8555 atoms |
| 1722 | | average map value = 0.0603, steps = 40 |
| 1723 | | shifted from previous position = 0.00252 |
| 1724 | | rotated from previous position = 0.00579 degrees |
| 1725 | | atoms outside contour = 6643, contour level = 0.10328 |
| 1726 | | |
| 1727 | | Position of 5zf6.pdb (#1) relative to |
| 1728 | | cryosparc_P1_J138_004_volume_map_sharp.mrc (#2) coordinates: |
| 1729 | | Matrix rotation and translation |
| 1730 | | -0.56226280 0.77254581 0.29501442 179.14915690 |
| 1731 | | 0.55849019 0.09164317 0.82443328 73.23928513 |
| 1732 | | 0.60987642 0.62831083 -0.48298680 60.24865553 |
| 1733 | | Axis -0.45793277 -0.73518163 -0.49980551 |
| 1734 | | Axis point 68.43839047 0.00000000 12.82706773 |
| 1735 | | Rotation angle (degrees) 167.63498549 |
| 1736 | | Shift along axis -165.99505628 |
| 1737 | | |
| 1738 | | |
| 1739 | | > fitmap #1 inMap #2 |
| 1740 | | |
| 1741 | | Fit molecule 5zf6.pdb (#1) to map cryosparc_P1_J138_004_volume_map_sharp.mrc |
| 1742 | | (#2) using 8555 atoms |
| 1743 | | average map value = 0.0603, steps = 44 |
| 1744 | | shifted from previous position = 0.0109 |
| 1745 | | rotated from previous position = 0.00848 degrees |
| 1746 | | atoms outside contour = 6643, contour level = 0.10328 |
| 1747 | | |
| 1748 | | Position of 5zf6.pdb (#1) relative to |
| 1749 | | cryosparc_P1_J138_004_volume_map_sharp.mrc (#2) coordinates: |
| 1750 | | Matrix rotation and translation |
| 1751 | | -0.56228174 0.77250668 0.29508080 179.14693278 |
| 1752 | | 0.55858918 0.09169034 0.82436097 73.22718572 |
| 1753 | | 0.60976829 0.62835206 -0.48306968 60.26918749 |
| 1754 | | Axis -0.45793423 -0.73520218 -0.49977394 |
| 1755 | | Axis point 68.43867717 0.00000000 12.83878493 |
| 1756 | | Rotation angle (degrees) 167.64229799 |
| 1757 | | Shift along axis -165.99526915 |
| 1758 | | |
| 1759 | | |
| 1760 | | > select add #3 |
| 1761 | | |
| 1762 | | 16299 atoms, 16424 bonds, 2168 residues, 3 models selected |
| 1763 | | |
| 1764 | | > fitmap #1 inMap #2 |
| 1765 | | |
| 1766 | | Fit molecule 5zf6.pdb (#1) to map cryosparc_P1_J138_004_volume_map_sharp.mrc |
| 1767 | | (#2) using 8555 atoms |
| 1768 | | average map value = 0.0603, steps = 48 |
| 1769 | | shifted from previous position = 0.00251 |
| 1770 | | rotated from previous position = 0.00327 degrees |
| 1771 | | atoms outside contour = 6644, contour level = 0.10328 |
| 1772 | | |
| 1773 | | Position of 5zf6.pdb (#1) relative to |
| 1774 | | cryosparc_P1_J138_004_volume_map_sharp.mrc (#2) coordinates: |
| 1775 | | Matrix rotation and translation |
| 1776 | | -0.56227363 0.77253040 0.29503414 179.14739408 |
| 1777 | | 0.55858437 0.09172832 0.82436000 73.22579110 |
| 1778 | | 0.60978018 0.62831735 -0.48309982 60.27170015 |
| 1779 | | Axis -0.45793523 -0.73521399 -0.49975566 |
| 1780 | | Axis point 68.43952822 -0.00000000 12.84303370 |
| 1781 | | Rotation angle (degrees) 167.64016407 |
| 1782 | | Shift along axis -165.99565204 |
| 1783 | | |
| 1784 | | |
| 1785 | | > fitmap #1 inMap #2 |
| 1786 | | |
| 1787 | | Fit molecule 5zf6.pdb (#1) to map cryosparc_P1_J138_004_volume_map_sharp.mrc |
| 1788 | | (#2) using 8555 atoms |
| 1789 | | average map value = 0.0603, steps = 40 |
| 1790 | | shifted from previous position = 0.00106 |
| 1791 | | rotated from previous position = 0.00975 degrees |
| 1792 | | atoms outside contour = 6643, contour level = 0.10328 |
| 1793 | | |
| 1794 | | Position of 5zf6.pdb (#1) relative to |
| 1795 | | cryosparc_P1_J138_004_volume_map_sharp.mrc (#2) coordinates: |
| 1796 | | Matrix rotation and translation |
| 1797 | | -0.56225268 0.77260373 0.29488202 179.15110347 |
| 1798 | | 0.55852278 0.09180948 0.82439270 73.22831911 |
| 1799 | | 0.60985591 0.62821532 -0.48313691 60.26806007 |
| 1800 | | Axis -0.45793261 -0.73523677 -0.49972454 |
| 1801 | | Axis point 68.44249251 0.00000000 12.84809670 |
| 1802 | | Rotation angle (degrees) 167.63146412 |
| 1803 | | Shift along axis -165.99671418 |
| 1804 | | |
| 1805 | | |
| 1806 | | > select add #4 |
| 1807 | | |
| 1808 | | 20982 atoms, 21139 bonds, 2845 residues, 3 models selected |
| 1809 | | |
| 1810 | | > select subtract #4 |
| 1811 | | |
| 1812 | | 12427 atoms, 12521 bonds, 1671 residues, 2 models selected |
| 1813 | | |
| 1814 | | > fitmap #1 inMap #2 |
| 1815 | | |
| 1816 | | Fit molecule 5zf6.pdb (#1) to map cryosparc_P1_J138_004_volume_map_sharp.mrc |
| 1817 | | (#2) using 8555 atoms |
| 1818 | | average map value = 0.0603, steps = 40 |
| 1819 | | shifted from previous position = 0.0118 |
| 1820 | | rotated from previous position = 0.00691 degrees |
| 1821 | | atoms outside contour = 6640, contour level = 0.10328 |
| 1822 | | |
| 1823 | | Position of 5zf6.pdb (#1) relative to |
| 1824 | | cryosparc_P1_J138_004_volume_map_sharp.mrc (#2) coordinates: |
| 1825 | | Matrix rotation and translation |
| 1826 | | -0.56224678 0.77262775 0.29483032 179.15681735 |
| 1827 | | 0.55843723 0.09177153 0.82445488 73.23752923 |
| 1828 | | 0.60993969 0.62819133 -0.48306235 60.24853618 |
| 1829 | | Axis -0.45792659 -0.73522035 -0.49975421 |
| 1830 | | Axis point 68.44485428 0.00000000 12.83738565 |
| 1831 | | Rotation angle (degrees) 167.62577932 |
| 1832 | | Shift along axis -165.99585229 |
| 1833 | | |
| 1834 | | |
| 1835 | | > select add #1 |
| 1836 | | |
| 1837 | | 17110 atoms, 17236 bonds, 2348 residues, 2 models selected |
| 1838 | | |
| 1839 | | > select subtract #1 |
| 1840 | | |
| 1841 | | 8555 atoms, 8618 bonds, 1174 residues, 1 model selected |
| 1842 | | |
| 1843 | | > select add #1 |
| 1844 | | |
| 1845 | | 17110 atoms, 17236 bonds, 2348 residues, 2 models selected |
| 1846 | | |
| 1847 | | > view matrix models |
| 1848 | | > #1,-0.56225,0.77263,0.29483,231.16,0.55844,0.091772,0.82445,121.3,0.60994,0.62819,-0.48306,94.371,#3,0.32146,-0.80003,-0.50657,136.35,0.63696,-0.21316,0.74085,-39.027,-0.70068,-0.56081,0.44107,322.02 |
| 1849 | | |
| 1850 | | > fitmap #1 inMap #2 |
| 1851 | | |
| 1852 | | Fit molecule 5zf6.pdb (#1) to map cryosparc_P1_J138_004_volume_map_sharp.mrc |
| 1853 | | (#2) using 8555 atoms |
| 1854 | | average map value = 0.1101, steps = 252 |
| 1855 | | shifted from previous position = 3.7 |
| 1856 | | rotated from previous position = 2.65 degrees |
| 1857 | | atoms outside contour = 5173, contour level = 0.10328 |
| 1858 | | |
| 1859 | | Position of 5zf6.pdb (#1) relative to |
| 1860 | | cryosparc_P1_J138_004_volume_map_sharp.mrc (#2) coordinates: |
| 1861 | | Matrix rotation and translation |
| 1862 | | -0.53022950 0.78334634 0.32438432 175.25685423 |
| 1863 | | 0.58887827 0.06499723 0.80560396 67.48236203 |
| 1864 | | 0.60998283 0.61817787 -0.49575909 63.99041146 |
| 1865 | | Axis -0.47681259 -0.72656353 -0.49472739 |
| 1866 | | Axis point 66.66494214 0.00000000 15.64059965 |
| 1867 | | Rotation angle (degrees) 168.66526300 |
| 1868 | | Shift along axis -164.25270661 |
| 1869 | | |
| 1870 | | |
| 1871 | | > view matrix models |
| 1872 | | > #1,-0.53023,0.78335,0.32438,227.93,0.58888,0.064997,0.8056,120.21,0.60998,0.61818,-0.49576,94.092,#3,0.32146,-0.80003,-0.50657,136.72,0.63696,-0.21316,0.74085,-35.748,-0.70068,-0.56081,0.44107,323.6 |
| 1873 | | |
| 1874 | | > select subtract #3 |
| 1875 | | |
| 1876 | | 8555 atoms, 8618 bonds, 1174 residues, 1 model selected |
| 1877 | | |
| 1878 | | > fitmap #1 inMap #2 |
| 1879 | | |
| 1880 | | Fit molecule 5zf6.pdb (#1) to map cryosparc_P1_J138_004_volume_map_sharp.mrc |
| 1881 | | (#2) using 8555 atoms |
| 1882 | | average map value = 0.06778, steps = 88 |
| 1883 | | shifted from previous position = 4.08 |
| 1884 | | rotated from previous position = 2.46 degrees |
| 1885 | | atoms outside contour = 6341, contour level = 0.10328 |
| 1886 | | |
| 1887 | | Position of 5zf6.pdb (#1) relative to |
| 1888 | | cryosparc_P1_J138_004_volume_map_sharp.mrc (#2) coordinates: |
| 1889 | | Matrix rotation and translation |
| 1890 | | -0.56182390 0.76213390 0.32172320 177.76957181 |
| 1891 | | 0.56503217 0.06947264 0.82213879 70.72361557 |
| 1892 | | 0.60422889 0.64368118 -0.46966157 68.59500191 |
| 1893 | | Axis -0.46000085 -0.72820014 -0.50805883 |
| 1894 | | Axis point 67.67791239 0.00000000 16.37816490 |
| 1895 | | Rotation angle (degrees) 168.81513008 |
| 1896 | | Shift along axis -168.12539753 |
| 1897 | | |
| 1898 | | |
| 1899 | | > close #3 |
| 1900 | | |
| 1901 | | > open |
| 1902 | | > /Users/madhurikanavalli/Desktop/ImageProcessing/kasiafiles/hPNP_dimer_5zf6_fit_tetramer2.pdb |
| 1903 | | |
| 1904 | | hPNP_dimer_5zf6_fit_tetramer2.pdb title: |
| 1905 | | Crystal structure of the dimeric human pnpase [more info...] |
| 1906 | | |
| 1907 | | Chain information for hPNP_dimer_5zf6_fit_tetramer2.pdb #3 |
| 1908 | | --- |
| 1909 | | Chain | Description | UniProt |
| 1910 | | A B | polyribonucleotide nucleotidyltransferase 1, mitochondrial | PNPT1_HUMAN 46-669 |
| 1911 | | |
| 1912 | | |
| 1913 | | > select add #3 |
| 1914 | | |
| 1915 | | 17110 atoms, 17236 bonds, 2348 residues, 2 models selected |
| 1916 | | |
| 1917 | | > select subtract #1 |
| 1918 | | |
| 1919 | | 8555 atoms, 8618 bonds, 1174 residues, 1 model selected |
| 1920 | | |
| 1921 | | > select subtract #3 |
| 1922 | | |
| 1923 | | Nothing selected |
| 1924 | | |
| 1925 | | > select add #3 |
| 1926 | | |
| 1927 | | 8555 atoms, 8618 bonds, 1174 residues, 1 model selected |
| 1928 | | |
| 1929 | | > view matrix models #3,1,0,0,-29.495,0,1,0,-74.165,0,0,1,7.8615 |
| 1930 | | |
| 1931 | | > view matrix models #3,1,0,0,-30.191,0,1,0,-73.496,0,0,1,19.042 |
| 1932 | | |
| 1933 | | > select add #4 |
| 1934 | | |
| 1935 | | 17110 atoms, 17236 bonds, 2348 residues, 2 models selected |
| 1936 | | |
| 1937 | | > view matrix models |
| 1938 | | > #4,0.43664,-0.89777,0.05801,169.54,0.24953,0.18281,0.95095,76.564,-0.86434,-0.40075,0.30385,338.08,#3,1,0,0,-33.957,0,1,0,-141.45,0,0,1,73.984 |
| 1939 | | |
| 1940 | | > select subtract #4 |
| 1941 | | |
| 1942 | | 8555 atoms, 8618 bonds, 1174 residues, 1 model selected |
| 1943 | | |
| 1944 | | > view matrix models #3,1,0,0,-6.382,0,1,0,-37.244,0,0,1,78.169 |
| 1945 | | |
| 1946 | | > view matrix models #3,1,0,0,7.1074,0,1,0,14.723,0,0,1,79.588 |
| 1947 | | |
| 1948 | | > ui mousemode right "rotate selected models" |
| 1949 | | |
| 1950 | | > view matrix models |
| 1951 | | > #3,-0.39782,0.91744,-0.0062689,97.728,0.85434,0.36795,-0.36705,23.539,-0.33444,-0.15137,-0.93018,435.14 |
| 1952 | | |
| 1953 | | > hide #!4 models |
| 1954 | | |
| 1955 | | > view matrix models |
| 1956 | | > #3,-0.64157,-0.026449,-0.76661,423.28,0.15277,0.97498,-0.16149,13.079,0.7517,-0.22072,-0.62148,203.2 |
| 1957 | | |
| 1958 | | > ui mousemode right "translate selected models" |
| 1959 | | |
| 1960 | | > view matrix models |
| 1961 | | > #3,-0.64157,-0.026449,-0.76661,427.84,0.15277,0.97498,-0.16149,14.341,0.7517,-0.22072,-0.62148,195.08 |
| 1962 | | |
| 1963 | | > fitmap #1 inMap #2 |
| 1964 | | |
| 1965 | | Fit molecule 5zf6.pdb (#1) to map cryosparc_P1_J138_004_volume_map_sharp.mrc |
| 1966 | | (#2) using 8555 atoms |
| 1967 | | average map value = 0.06778, steps = 44 |
| 1968 | | shifted from previous position = 0.014 |
| 1969 | | rotated from previous position = 0.0147 degrees |
| 1970 | | atoms outside contour = 6338, contour level = 0.10328 |
| 1971 | | |
| 1972 | | Position of 5zf6.pdb (#1) relative to |
| 1973 | | cryosparc_P1_J138_004_volume_map_sharp.mrc (#2) coordinates: |
| 1974 | | Matrix rotation and translation |
| 1975 | | -0.56177320 0.76214508 0.32178525 177.76212068 |
| 1976 | | 0.56493063 0.06925504 0.82222693 70.73071438 |
| 1977 | | 0.60437096 0.64369139 -0.46946473 68.59002634 |
| 1978 | | Axis -0.46002220 -0.72812228 -0.50815109 |
| 1979 | | Axis point 67.67059892 0.00000000 16.36862818 |
| 1980 | | Rotation angle (degrees) 168.81070899 |
| 1981 | | Shift along axis -168.12922648 |
| 1982 | | |
| 1983 | | |
| 1984 | | > fitmap #1 inMap #2 |
| 1985 | | |
| 1986 | | Fit molecule 5zf6.pdb (#1) to map cryosparc_P1_J138_004_volume_map_sharp.mrc |
| 1987 | | (#2) using 8555 atoms |
| 1988 | | average map value = 0.06778, steps = 44 |
| 1989 | | shifted from previous position = 0.00118 |
| 1990 | | rotated from previous position = 0.00555 degrees |
| 1991 | | atoms outside contour = 6338, contour level = 0.10328 |
| 1992 | | |
| 1993 | | Position of 5zf6.pdb (#1) relative to |
| 1994 | | cryosparc_P1_J138_004_volume_map_sharp.mrc (#2) coordinates: |
| 1995 | | Matrix rotation and translation |
| 1996 | | -0.56180042 0.76212365 0.32178849 177.76456401 |
| 1997 | | 0.56497732 0.06932409 0.82218903 70.72650702 |
| 1998 | | 0.60430201 0.64370934 -0.46952888 68.59710591 |
| 1999 | | Axis -0.46001209 -0.72814803 -0.50812333 |
| 2000 | | Axis point 67.67325732 0.00000000 16.37390083 |
| 2001 | | Rotation angle (degrees) 168.81400533 |
| 2002 | | Shift along axis -168.12900552 |
| 2003 | | |
| 2004 | | |
| 2005 | | > fitmap #1 inMap #2 |
| 2006 | | |
| 2007 | | Fit molecule 5zf6.pdb (#1) to map cryosparc_P1_J138_004_volume_map_sharp.mrc |
| 2008 | | (#2) using 8555 atoms |
| 2009 | | average map value = 0.06778, steps = 44 |
| 2010 | | shifted from previous position = 0.0011 |
| 2011 | | rotated from previous position = 0.00219 degrees |
| 2012 | | atoms outside contour = 6340, contour level = 0.10328 |
| 2013 | | |
| 2014 | | Position of 5zf6.pdb (#1) relative to |
| 2015 | | cryosparc_P1_J138_004_volume_map_sharp.mrc (#2) coordinates: |
| 2016 | | Matrix rotation and translation |
| 2017 | | -0.56181677 0.76211115 0.32178954 177.76628394 |
| 2018 | | 0.56499162 0.06935189 0.82217686 70.72533923 |
| 2019 | | 0.60427344 0.64372114 -0.46954946 68.59902440 |
| 2020 | | Axis -0.46000509 -0.72815841 -0.50811479 |
| 2021 | | Axis point 67.67475916 0.00000000 16.37531817 |
| 2022 | | Rotation angle (degrees) 168.81535483 |
| 2023 | | Shift along axis -168.12882574 |
| 2024 | | |
| 2025 | | |
| 2026 | | > view matrix models |
| 2027 | | > #3,-0.64157,-0.026449,-0.76661,428.14,0.15277,0.97498,-0.16149,13.695,0.7517,-0.22072,-0.62148,197.78 |
| 2028 | | |
| 2029 | | > view matrix models |
| 2030 | | > #3,-0.64157,-0.026449,-0.76661,429.22,0.15277,0.97498,-0.16149,10.389,0.7517,-0.22072,-0.62148,201.96 |
| 2031 | | |
| 2032 | | > ui mousemode right "rotate selected models" |
| 2033 | | |
| 2034 | | > view matrix models |
| 2035 | | > #3,-0.91507,0.12718,-0.38272,398.89,0.29913,0.85053,-0.43258,43.498,0.2705,-0.51032,-0.81633,372.32 |
| 2036 | | |
| 2037 | | > view matrix models |
| 2038 | | > #3,-0.93066,0.25206,-0.26522,362.41,0.34092,0.86054,-0.37847,26.413,0.13284,-0.44264,-0.8868,395.06 |
| 2039 | | |
| 2040 | | > fitmap #1 inMap #2 |
| 2041 | | |
| 2042 | | Fit molecule 5zf6.pdb (#1) to map cryosparc_P1_J138_004_volume_map_sharp.mrc |
| 2043 | | (#2) using 8555 atoms |
| 2044 | | average map value = 0.06778, steps = 44 |
| 2045 | | shifted from previous position = 0.00109 |
| 2046 | | rotated from previous position = 0.0011 degrees |
| 2047 | | atoms outside contour = 6339, contour level = 0.10328 |
| 2048 | | |
| 2049 | | Position of 5zf6.pdb (#1) relative to |
| 2050 | | cryosparc_P1_J138_004_volume_map_sharp.mrc (#2) coordinates: |
| 2051 | | Matrix rotation and translation |
| 2052 | | -0.56180508 0.76212041 0.32178802 177.76501259 |
| 2053 | | 0.56498774 0.06933961 0.82218056 70.72555730 |
| 2054 | | 0.60428793 0.64371150 -0.46954404 68.59878002 |
| 2055 | | Axis -0.46001046 -0.72815376 -0.50811660 |
| 2056 | | Axis point 67.67377478 0.00000000 16.37519960 |
| 2057 | | Rotation angle (degrees) 168.81464010 |
| 2058 | | Shift along axis -168.12902480 |
| 2059 | | |
| 2060 | | |
| 2061 | | > fitmap #1 inMap #2 |
| 2062 | | |
| 2063 | | Fit molecule 5zf6.pdb (#1) to map cryosparc_P1_J138_004_volume_map_sharp.mrc |
| 2064 | | (#2) using 8555 atoms |
| 2065 | | average map value = 0.06778, steps = 44 |
| 2066 | | shifted from previous position = 0.0013 |
| 2067 | | rotated from previous position = 0.00174 degrees |
| 2068 | | atoms outside contour = 6340, contour level = 0.10328 |
| 2069 | | |
| 2070 | | Position of 5zf6.pdb (#1) relative to |
| 2071 | | cryosparc_P1_J138_004_volume_map_sharp.mrc (#2) coordinates: |
| 2072 | | Matrix rotation and translation |
| 2073 | | -0.56182107 0.76210821 0.32178898 177.76676058 |
| 2074 | | 0.56499631 0.06936111 0.82217285 70.72493750 |
| 2075 | | 0.60426505 0.64372363 -0.46955686 68.59974743 |
| 2076 | | Axis -0.46000327 -0.72816181 -0.50811158 |
| 2077 | | Axis point 67.67518917 0.00000000 16.37588980 |
| 2078 | | Rotation angle (degrees) 168.81572170 |
| 2079 | | Shift along axis -168.12881525 |
| 2080 | | |
| 2081 | | |
| 2082 | | > fitmap #1 inMap #2 |
| 2083 | | |
| 2084 | | Fit molecule 5zf6.pdb (#1) to map cryosparc_P1_J138_004_volume_map_sharp.mrc |
| 2085 | | (#2) using 8555 atoms |
| 2086 | | average map value = 0.06778, steps = 44 |
| 2087 | | shifted from previous position = 0.0015 |
| 2088 | | rotated from previous position = 0.00184 degrees |
| 2089 | | atoms outside contour = 6339, contour level = 0.10328 |
| 2090 | | |
| 2091 | | Position of 5zf6.pdb (#1) relative to |
| 2092 | | cryosparc_P1_J138_004_volume_map_sharp.mrc (#2) coordinates: |
| 2093 | | Matrix rotation and translation |
| 2094 | | -0.56180367 0.76212182 0.32178714 177.76486802 |
| 2095 | | 0.56498749 0.06933911 0.82218077 70.72556637 |
| 2096 | | 0.60428947 0.64370988 -0.46954426 68.59886768 |
| 2097 | | Axis -0.46001109 -0.72815353 -0.50811636 |
| 2098 | | Axis point 67.67366634 -0.00000000 16.37528860 |
| 2099 | | Rotation angle (degrees) 168.81453944 |
| 2100 | | Shift along axis -168.12908826 |
| 2101 | | |
| 2102 | | |
| 2103 | | > fitmap #1 inMap #2 |
| 2104 | | |
| 2105 | | Fit molecule 5zf6.pdb (#1) to map cryosparc_P1_J138_004_volume_map_sharp.mrc |
| 2106 | | (#2) using 8555 atoms |
| 2107 | | average map value = 0.06778, steps = 44 |
| 2108 | | shifted from previous position = 0.00161 |
| 2109 | | rotated from previous position = 0.00195 degrees |
| 2110 | | atoms outside contour = 6340, contour level = 0.10328 |
| 2111 | | |
| 2112 | | Position of 5zf6.pdb (#1) relative to |
| 2113 | | cryosparc_P1_J138_004_volume_map_sharp.mrc (#2) coordinates: |
| 2114 | | Matrix rotation and translation |
| 2115 | | -0.56182168 0.76210791 0.32178865 177.76684825 |
| 2116 | | 0.56499718 0.06936289 0.82217211 70.72490535 |
| 2117 | | 0.60426368 0.64372379 -0.46955839 68.59980313 |
| 2118 | | Axis -0.46000301 -0.72816245 -0.50811088 |
| 2119 | | Axis point 67.67525750 0.00000000 16.37595603 |
| 2120 | | Rotation angle (degrees) 168.81577332 |
| 2121 | | Shift along axis -168.12881302 |
| 2122 | | |
| 2123 | | |
| 2124 | | > fitmap #1 inMap #2 |
| 2125 | | |
| 2126 | | Fit molecule 5zf6.pdb (#1) to map cryosparc_P1_J138_004_volume_map_sharp.mrc |
| 2127 | | (#2) using 8555 atoms |
| 2128 | | average map value = 0.06778, steps = 44 |
| 2129 | | shifted from previous position = 0.0017 |
| 2130 | | rotated from previous position = 0.00202 degrees |
| 2131 | | atoms outside contour = 6339, contour level = 0.10328 |
| 2132 | | |
| 2133 | | Position of 5zf6.pdb (#1) relative to |
| 2134 | | cryosparc_P1_J138_004_volume_map_sharp.mrc (#2) coordinates: |
| 2135 | | Matrix rotation and translation |
| 2136 | | -0.56180300 0.76212254 0.32178662 177.76481731 |
| 2137 | | 0.56498709 0.06933868 0.82218108 70.72559720 |
| 2138 | | 0.60429047 0.64370908 -0.46954407 68.59886938 |
| 2139 | | Axis -0.46001136 -0.72815335 -0.50811637 |
| 2140 | | Axis point 67.67362036 0.00000000 16.37530466 |
| 2141 | | Rotation angle (degrees) 168.81447555 |
| 2142 | | Shift along axis -168.12912509 |
| 2143 | | |
| 2144 | | |
| 2145 | | > fitmap #1 inMap #2 |
| 2146 | | |
| 2147 | | Fit molecule 5zf6.pdb (#1) to map cryosparc_P1_J138_004_volume_map_sharp.mrc |
| 2148 | | (#2) using 8555 atoms |
| 2149 | | average map value = 0.06778, steps = 44 |
| 2150 | | shifted from previous position = 0.00172 |
| 2151 | | rotated from previous position = 0.00207 degrees |
| 2152 | | atoms outside contour = 6340, contour level = 0.10328 |
| 2153 | | |
| 2154 | | Position of 5zf6.pdb (#1) relative to |
| 2155 | | cryosparc_P1_J138_004_volume_map_sharp.mrc (#2) coordinates: |
| 2156 | | Matrix rotation and translation |
| 2157 | | -0.56182263 0.76210752 0.32178790 177.76699161 |
| 2158 | | 0.56499677 0.06936407 0.82217229 70.72494421 |
| 2159 | | 0.60426317 0.64372412 -0.46955859 68.59983213 |
| 2160 | | Axis -0.46000248 -0.72816286 -0.50811078 |
| 2161 | | Axis point 67.67535296 0.00000000 16.37598872 |
| 2162 | | Rotation angle (degrees) 168.81576963 |
| 2163 | | Shift along axis -168.12884954 |
| 2164 | | |
| 2165 | | |
| 2166 | | > fitmap #1 inMap #2 |
| 2167 | | |
| 2168 | | Fit molecule 5zf6.pdb (#1) to map cryosparc_P1_J138_004_volume_map_sharp.mrc |
| 2169 | | (#2) using 8555 atoms |
| 2170 | | average map value = 0.06778, steps = 44 |
| 2171 | | shifted from previous position = 0.00176 |
| 2172 | | rotated from previous position = 0.00204 degrees |
| 2173 | | atoms outside contour = 6338, contour level = 0.10328 |
| 2174 | | |
| 2175 | | Position of 5zf6.pdb (#1) relative to |
| 2176 | | cryosparc_P1_J138_004_volume_map_sharp.mrc (#2) coordinates: |
| 2177 | | Matrix rotation and translation |
| 2178 | | -0.56180310 0.76212248 0.32178657 177.76482158 |
| 2179 | | 0.56498745 0.06933920 0.82218079 70.72554566 |
| 2180 | | 0.60429004 0.64370910 -0.46954462 68.59896224 |
| 2181 | | Axis -0.46001133 -0.72815354 -0.50811613 |
| 2182 | | Axis point 67.67363504 0.00000000 16.37537496 |
| 2183 | | Rotation angle (degrees) 168.81449424 |
| 2184 | | Shift along axis -168.12912781 |
| 2185 | | |
| 2186 | | |
| 2187 | | > select add #1 |
| 2188 | | |
| 2189 | | 17110 atoms, 17236 bonds, 2348 residues, 2 models selected |
| 2190 | | |
| 2191 | | > view matrix models |
| 2192 | | > #1,-0.14985,0.98863,0.012626,183.06,0.38156,0.046045,0.9232,139.05,0.91212,0.14316,-0.38412,80.456,#3,-0.71358,-0.03176,-0.69986,455.85,0.2007,0.94783,-0.24765,11.335,0.67121,-0.31718,-0.66998,236.49 |
| 2193 | | |
| 2194 | | > view matrix models |
| 2195 | | > #1,0.70687,0.28057,0.64932,108.08,0.4226,-0.90364,-0.069595,177.3,0.56723,0.3236,-0.75732,116.2,#3,0.34115,0.69223,-0.63595,112.39,0.88425,-0.0067957,0.46696,-46.86,0.31892,-0.72165,-0.61442,370.77 |
| 2196 | | |
| 2197 | | > view matrix models |
| 2198 | | > #1,0.148,-0.90826,0.39135,209.41,0.70956,0.37316,0.59772,89.53,-0.68892,0.18923,0.6997,243.87,#3,0.63093,0.4315,0.64478,-110.94,0.29194,0.63795,-0.7126,129.47,-0.71882,0.63783,0.27653,139.68 |
| 2199 | | |
| 2200 | | > select add #2 |
| 2201 | | |
| 2202 | | 17110 atoms, 17236 bonds, 2348 residues, 4 models selected |
| 2203 | | |
| 2204 | | > hide #!3 models |
| 2205 | | |
| 2206 | | > fitmap #1 inMap #2 |
| 2207 | | |
| 2208 | | Fit molecule 5zf6.pdb (#1) to map cryosparc_P1_J138_004_volume_map_sharp.mrc |
| 2209 | | (#2) using 8555 atoms |
| 2210 | | average map value = 0.04801, steps = 284 |
| 2211 | | shifted from previous position = 22 |
| 2212 | | rotated from previous position = 25 degrees |
| 2213 | | atoms outside contour = 7189, contour level = 0.10328 |
| 2214 | | |
| 2215 | | Position of 5zf6.pdb (#1) relative to |
| 2216 | | cryosparc_P1_J138_004_volume_map_sharp.mrc (#2) coordinates: |
| 2217 | | Matrix rotation and translation |
| 2218 | | 0.38285300 -0.90468146 0.18701612 126.18295836 |
| 2219 | | 0.48235861 0.36841712 0.79473202 61.45913081 |
| 2220 | | -0.78787927 -0.21405670 0.57743049 239.44909763 |
| 2221 | | Axis -0.51134765 0.49416737 0.70308050 |
| 2222 | | Axis point 154.24471051 110.10948885 0.00000000 |
| 2223 | | Rotation angle (degrees) 80.54050570 |
| 2224 | | Shift along axis 134.19972853 |
| 2225 | | |
| 2226 | | |
| 2227 | | > view matrix models |
| 2228 | | > #2,0.9936,0.053643,0.099376,30.867,-0.051054,0.99829,-0.028415,60.494,-0.10073,0.023159,0.99464,39.57,#1,0.32798,-0.9004,0.28583,183.34,0.48438,0.42006,0.76742,108.6,-0.81105,-0.11325,0.57391,266.45,#3,0.57112,0.52635,0.62991,-115.99,0.27965,0.5967,-0.75216,145.55,-0.77176,0.60573,0.19359,168.43 |
| 2229 | | |
| 2230 | | > select subtract #3 |
| 2231 | | |
| 2232 | | 8555 atoms, 8618 bonds, 1174 residues, 3 models selected |
| 2233 | | |
| 2234 | | > view matrix models |
| 2235 | | > #2,0.96755,0.032451,0.25059,15.256,-0.015944,0.99758,-0.067624,60.534,-0.25218,0.061434,0.96573,59.216,#1,0.18865,-0.91701,0.35144,199.34,0.52837,0.39643,0.75078,103.64,-0.82779,0.044056,0.5593,262.41 |
| 2236 | | |
| 2237 | | > select subtract #2 |
| 2238 | | |
| 2239 | | 8555 atoms, 8618 bonds, 1174 residues, 1 model selected |
| 2240 | | |
| 2241 | | > view matrix models |
| 2242 | | > #1,0.64787,-0.38882,0.65504,144.54,0.036303,0.8747,0.4833,147.87,-0.76089,-0.28934,0.58081,260.13 |
| 2243 | | |
| 2244 | | > ui mousemode right "translate selected models" |
| 2245 | | |
| 2246 | | > view matrix models |
| 2247 | | > #1,0.64787,-0.38882,0.65504,135.53,0.036303,0.8747,0.4833,160.4,-0.76089,-0.28934,0.58081,260.02 |
| 2248 | | |
| 2249 | | > ui mousemode right "rotate selected models" |
| 2250 | | |
| 2251 | | > view matrix models |
| 2252 | | > #1,0.23258,-0.84668,0.47859,181.53,0.70575,0.48551,0.51595,108.3,-0.6692,0.21777,0.71046,241.92 |
| 2253 | | |
| 2254 | | > view matrix models |
| 2255 | | > #1,0.49293,-0.85966,-0.13421,180.79,0.52373,0.16999,0.83475,116.43,-0.69478,-0.48176,0.53402,258.42 |
| 2256 | | |
| 2257 | | > ui mousemode right "move picked models" |
| 2258 | | |
| 2259 | | > view matrix models |
| 2260 | | > #2,0.96755,0.032451,0.25059,16.908,-0.015944,0.99758,-0.067624,59.82,-0.25218,0.061434,0.96573,63.354 |
| 2261 | | |
| 2262 | | > fitmap #1 inMap #2 |
| 2263 | | |
| 2264 | | Fit molecule 5zf6.pdb (#1) to map cryosparc_P1_J138_004_volume_map_sharp.mrc |
| 2265 | | (#2) using 8555 atoms |
| 2266 | | average map value = 0.1297, steps = 808 |
| 2267 | | shifted from previous position = 2.33 |
| 2268 | | rotated from previous position = 6.32 degrees |
| 2269 | | atoms outside contour = 4645, contour level = 0.10328 |
| 2270 | | |
| 2271 | | Position of 5zf6.pdb (#1) relative to |
| 2272 | | cryosparc_P1_J138_004_volume_map_sharp.mrc (#2) coordinates: |
| 2273 | | Matrix rotation and translation |
| 2274 | | 0.57319897 -0.77448652 -0.26760710 116.68481785 |
| 2275 | | 0.55163281 0.12322629 0.82493425 69.43518167 |
| 2276 | | -0.60592423 -0.62047232 0.49786536 223.43458404 |
| 2277 | | Axis -0.72613777 0.16996245 0.66621070 |
| 2278 | | Axis point 0.00000000 179.57744939 122.33493989 |
| 2279 | | Rotation angle (degrees) 84.42519184 |
| 2280 | | Shift along axis 75.92663194 |
| 2281 | | |
| 2282 | | |
| 2283 | | > select subtract #1 |
| 2284 | | |
| 2285 | | Nothing selected |
| 2286 | | |
| 2287 | | > ui tool show "Model Loops" |
| 2288 | | |
| 2289 | | > ui tool show "Fit to Segments" |
| 2290 | | |
| 2291 | | Density map not found or not selected |
| 2292 | | [Repeated 3 time(s)] |
| 2293 | | |
| 2294 | | > select add #2 |
| 2295 | | |
| 2296 | | 2 models selected |
| 2297 | | |
| 2298 | | > fitmap #1 inMap #2 |
| 2299 | | |
| 2300 | | Fit molecule 5zf6.pdb (#1) to map cryosparc_P1_J138_004_volume_map_sharp.mrc |
| 2301 | | (#2) using 8555 atoms |
| 2302 | | average map value = 0.1297, steps = 144 |
| 2303 | | shifted from previous position = 0.0143 |
| 2304 | | rotated from previous position = 0.0137 degrees |
| 2305 | | atoms outside contour = 4641, contour level = 0.10328 |
| 2306 | | |
| 2307 | | Position of 5zf6.pdb (#1) relative to |
| 2308 | | cryosparc_P1_J138_004_volume_map_sharp.mrc (#2) coordinates: |
| 2309 | | Matrix rotation and translation |
| 2310 | | 0.57308626 -0.77449802 -0.26781512 116.69540535 |
| 2311 | | 0.55179062 0.12306769 0.82485238 69.42161691 |
| 2312 | | -0.60588715 -0.62048944 0.49788915 223.41907541 |
| 2313 | | Axis -0.72609643 0.16983726 0.66628768 |
| 2314 | | Axis point 0.00000000 179.54103153 122.34637292 |
| 2315 | | Rotation angle (degrees) 84.43231602 |
| 2316 | | Shift along axis 75.91963727 |
| 2317 | | |
| 2318 | | |
| 2319 | | > fitmap #1 inMap #2 |
| 2320 | | |
| 2321 | | Fit molecule 5zf6.pdb (#1) to map cryosparc_P1_J138_004_volume_map_sharp.mrc |
| 2322 | | (#2) using 8555 atoms |
| 2323 | | average map value = 0.129, steps = 2000 |
| 2324 | | shifted from previous position = 0.23 |
| 2325 | | rotated from previous position = 0.448 degrees |
| 2326 | | atoms outside contour = 4639, contour level = 0.10328 |
| 2327 | | |
| 2328 | | Position of 5zf6.pdb (#1) relative to |
| 2329 | | cryosparc_P1_J138_004_volume_map_sharp.mrc (#2) coordinates: |
| 2330 | | Matrix rotation and translation |
| 2331 | | 0.57258795 -0.77437910 -0.26922116 116.75226867 |
| 2332 | | 0.54793977 0.11719931 0.82826706 69.68325907 |
| 2333 | | -0.60984017 -0.62177272 0.49142002 223.76402791 |
| 2334 | | Axis -0.72801419 0.17101288 0.66389000 |
| 2335 | | Axis point 0.00000000 179.16562442 122.41380851 |
| 2336 | | Rotation angle (degrees) 84.80166483 |
| 2337 | | Shift along axis 75.47412571 |
| 2338 | | |
| 2339 | | |
| 2340 | | > fitmap #1 inMap #2 |
| 2341 | | |
| 2342 | | Fit molecule 5zf6.pdb (#1) to map cryosparc_P1_J138_004_volume_map_sharp.mrc |
| 2343 | | (#2) using 8555 atoms |
| 2344 | | average map value = 0.1277, steps = 2000 |
| 2345 | | shifted from previous position = 0.609 |
| 2346 | | rotated from previous position = 0.904 degrees |
| 2347 | | atoms outside contour = 4682, contour level = 0.10328 |
| 2348 | | |
| 2349 | | Position of 5zf6.pdb (#1) relative to |
| 2350 | | cryosparc_P1_J138_004_volume_map_sharp.mrc (#2) coordinates: |
| 2351 | | Matrix rotation and translation |
| 2352 | | 0.57348064 -0.77417828 -0.26789537 116.57776864 |
| 2353 | | 0.55651791 0.12818804 0.82088709 68.70481792 |
| 2354 | | -0.60117198 -0.61985143 0.50435745 223.01355175 |
| 2355 | | Axis -0.72422213 0.16752956 0.66890669 |
| 2356 | | Axis point 0.00000000 179.52079300 122.52462323 |
| 2357 | | Rotation angle (degrees) 84.08729719 |
| 2358 | | Shift along axis 76.25714444 |
| 2359 | | |
| 2360 | | |
| 2361 | | > fitmap #1 inMap #2 |
| 2362 | | |
| 2363 | | Fit molecule 5zf6.pdb (#1) to map cryosparc_P1_J138_004_volume_map_sharp.mrc |
| 2364 | | (#2) using 8555 atoms |
| 2365 | | average map value = 0.129, steps = 2000 |
| 2366 | | shifted from previous position = 0.398 |
| 2367 | | rotated from previous position = 0.192 degrees |
| 2368 | | atoms outside contour = 4689, contour level = 0.10328 |
| 2369 | | |
| 2370 | | Position of 5zf6.pdb (#1) relative to |
| 2371 | | cryosparc_P1_J138_004_volume_map_sharp.mrc (#2) coordinates: |
| 2372 | | Matrix rotation and translation |
| 2373 | | 0.57102733 -0.77539121 -0.26962241 116.96381429 |
| 2374 | | 0.55768186 0.12537938 0.82053089 68.95823782 |
| 2375 | | -0.60242736 -0.61890909 0.50401667 222.89061469 |
| 2376 | | Axis -0.72336130 0.16724436 0.66990877 |
| 2377 | | Axis point 0.00000000 179.32454376 122.64471433 |
| 2378 | | Rotation angle (degrees) 84.24863936 |
| 2379 | | Shift along axis 76.24215771 |
| 2380 | | |
| 2381 | | |
| 2382 | | > hide #!1 models |
| 2383 | | |
| 2384 | | > show #!1 models |
| 2385 | | |
| 2386 | | > select add #1 |
| 2387 | | |
| 2388 | | 8555 atoms, 8618 bonds, 1174 residues, 3 models selected |
| 2389 | | |
| 2390 | | > ui tool show "Show Sequence Viewer" |
| 2391 | | |
| 2392 | | > select subtract #2 |
| 2393 | | |
| 2394 | | 8555 atoms, 8618 bonds, 1174 residues, 1 model selected |
| 2395 | | |
| 2396 | | > hide #!2 models |
| 2397 | | |
| 2398 | | > select subtract #1 |
| 2399 | | |
| 2400 | | Nothing selected |
| 2401 | | |
| 2402 | | > select #1/A |
| 2403 | | |
| 2404 | | 4328 atoms, 4357 bonds, 597 residues, 1 model selected |
| 2405 | | |
| 2406 | | > select #1/B |
| 2407 | | |
| 2408 | | 4227 atoms, 4261 bonds, 577 residues, 1 model selected |
| 2409 | | |
| 2410 | | > ui tool show "Show Sequence Viewer" |
| 2411 | | |
| 2412 | | [Repeated 1 time(s)] |
| 2413 | | |
| 2414 | | > select add #1 |
| 2415 | | |
| 2416 | | 8555 atoms, 8618 bonds, 1174 residues, 1 model selected |
| 2417 | | |
| 2418 | | > select subtract #1 |
| 2419 | | |
| 2420 | | Nothing selected |
| 2421 | | |
| 2422 | | > ui tool show "Show Sequence Viewer" |
| 2423 | | |
| 2424 | | > sequence chain #1/B |
| 2425 | | |
| 2426 | | Alignment identifier is 1/B |
| 2427 | | |
| 2428 | | > select #1/B:300 |
| 2429 | | |
| 2430 | | 7 atoms, 6 bonds, 1 residue, 1 model selected |
| 2431 | | |
| 2432 | | > select #1/B:300-304 |
| 2433 | | |
| 2434 | | 46 atoms, 47 bonds, 5 residues, 1 model selected |
| 2435 | | |
| 2436 | | > select #1/B:299-300 |
| 2437 | | |
| 2438 | | 18 atoms, 18 bonds, 2 residues, 1 model selected |
| 2439 | | |
| 2440 | | > select #1/B:295-300 |
| 2441 | | |
| 2442 | | 50 atoms, 51 bonds, 6 residues, 1 model selected |
| 2443 | | |
| 2444 | | > select #1/B:295 |
| 2445 | | |
| 2446 | | 8 atoms, 7 bonds, 1 residue, 1 model selected |
| 2447 | | |
| 2448 | | > select #1/B:285-295 |
| 2449 | | |
| 2450 | | 96 atoms, 97 bonds, 11 residues, 1 model selected |
| 2451 | | |
| 2452 | | > select #1/B:285 |
| 2453 | | |
| 2454 | | 9 atoms, 8 bonds, 1 residue, 1 model selected |
| 2455 | | |
| 2456 | | > select #1/B:280-285 |
| 2457 | | |
| 2458 | | 46 atoms, 46 bonds, 6 residues, 1 model selected |
| 2459 | | |
| 2460 | | > select #1/B:279 |
| 2461 | | |
| 2462 | | 7 atoms, 7 bonds, 1 residue, 1 model selected |
| 2463 | | |
| 2464 | | > select #1/B:279-319 |
| 2465 | | |
| 2466 | | 343 atoms, 350 bonds, 41 residues, 1 model selected |
| 2467 | | |
| 2468 | | > select #1/B:320 |
| 2469 | | |
| 2470 | | 7 atoms, 6 bonds, 1 residue, 1 model selected |
| 2471 | | |
| 2472 | | > select #1/B:320-322 |
| 2473 | | |
| 2474 | | 25 atoms, 24 bonds, 3 residues, 1 model selected |
| 2475 | | |
| 2476 | | > select #1/B:277-278 |
| 2477 | | |
| 2478 | | 18 atoms, 18 bonds, 2 residues, 1 model selected |
| 2479 | | |
| 2480 | | > select #1/B:277-288 |
| 2481 | | |
| 2482 | | 100 atoms, 104 bonds, 12 residues, 1 model selected |
| 2483 | | |
| 2484 | | > select #1/B:277-278 |
| 2485 | | |
| 2486 | | 18 atoms, 18 bonds, 2 residues, 1 model selected |
| 2487 | | |
| 2488 | | > select #1/B:277-307 |
| 2489 | | |
| 2490 | | 263 atoms, 271 bonds, 31 residues, 1 model selected |
| 2491 | | |
| 2492 | | > select #1/B:279-319 |
| 2493 | | |
| 2494 | | 343 atoms, 350 bonds, 41 residues, 1 model selected |
| 2495 | | |
| 2496 | | > select #1/B:277 |
| 2497 | | |
| 2498 | | 11 atoms, 11 bonds, 1 residue, 1 model selected |
| 2499 | | |
| 2500 | | > select #1/B:277-335 |
| 2501 | | |
| 2502 | | 498 atoms, 510 bonds, 59 residues, 1 model selected |
| 2503 | | |
| 2504 | | > select add #2 |
| 2505 | | |
| 2506 | | 498 atoms, 510 bonds, 59 residues, 3 models selected |
| 2507 | | |
| 2508 | | > show #!2 models |
| 2509 | | |
| 2510 | | > hide #!2 models |
| 2511 | | |
| 2512 | | > show #!2 models |
| 2513 | | |
| 2514 | | > ui tool show "Fit to Segments" |
| 2515 | | |
| 2516 | | Density map not found or not selected |
| 2517 | | [Repeated 6 time(s)] |
| 2518 | | |
| 2519 | | > select subtract #2 |
| 2520 | | |
| 2521 | | 498 atoms, 510 bonds, 59 residues, 1 model selected |
| 2522 | | |
| 2523 | | > fitmap #1 inMap #2 |
| 2524 | | |
| 2525 | | Fit molecule 5zf6.pdb (#1) to map cryosparc_P1_J138_004_volume_map_sharp.mrc |
| 2526 | | (#2) using 8555 atoms |
| 2527 | | average map value = 0.1289, steps = 2000 |
| 2528 | | shifted from previous position = 0.257 |
| 2529 | | rotated from previous position = 0.915 degrees |
| 2530 | | atoms outside contour = 4632, contour level = 0.10328 |
| 2531 | | |
| 2532 | | Position of 5zf6.pdb (#1) relative to |
| 2533 | | cryosparc_P1_J138_004_volume_map_sharp.mrc (#2) coordinates: |
| 2534 | | Matrix rotation and translation |
| 2535 | | 0.57267166 -0.77461823 -0.26835379 116.74682003 |
| 2536 | | 0.54692091 0.11716150 0.82894553 69.74141727 |
| 2537 | | -0.61067559 -0.62148191 0.49074998 223.83938491 |
| 2538 | | Axis -0.72818810 0.17186290 0.66347964 |
| 2539 | | Axis point 0.00000000 179.26106398 122.43296113 |
| 2540 | | Rotation angle (degrees) 84.81961892 |
| 2541 | | Shift along axis 75.48519232 |
| 2542 | | |
| 2543 | | |
| 2544 | | > select add #2 |
| 2545 | | |
| 2546 | | 498 atoms, 510 bonds, 59 residues, 3 models selected |
| 2547 | | |
| 2548 | | > ui tool show "Fit to Segments" |
| 2549 | | |
| 2550 | | Simulating map res 4.000, grid 2.000 |
| 2551 | | |
| 2552 | | > molmap #1 4.000000 sigmaFactor 0.187 gridSpacing 2.000000 replace false |
| 2553 | | |
| 2554 | | Opened 5zf6.pdb map 4 as #5, grid size 47,52,63, pixel 2, shown at level |
| 2555 | | 0.104, step 1, values float32 |
| 2556 | | Please select an open structure to fit |
| 2557 | | |
| 2558 | | > select subtract #2 |
| 2559 | | |
| 2560 | | 498 atoms, 510 bonds, 59 residues, 1 model selected |
| 2561 | | |
| 2562 | | > fitmap #1 inMap #2 |
| 2563 | | |
| 2564 | | Fit molecule 5zf6.pdb (#1) to map cryosparc_P1_J138_004_volume_map_sharp.mrc |
| 2565 | | (#2) using 8555 atoms |
| 2566 | | average map value = 0.1276, steps = 2000 |
| 2567 | | shifted from previous position = 0.642 |
| 2568 | | rotated from previous position = 1.03 degrees |
| 2569 | | atoms outside contour = 4675, contour level = 0.10328 |
| 2570 | | |
| 2571 | | Position of 5zf6.pdb (#1) relative to |
| 2572 | | cryosparc_P1_J138_004_volume_map_sharp.mrc (#2) coordinates: |
| 2573 | | Matrix rotation and translation |
| 2574 | | 0.95624965 0.12601838 -0.26401886 146.56566194 |
| 2575 | | 0.23327279 0.21618286 0.94807635 145.55677599 |
| 2576 | | 0.17655140 -0.96818610 0.17732813 184.05932290 |
| 2577 | | Axis -0.97312742 -0.22373293 0.05446655 |
| 2578 | | Axis point 0.00000000 174.29413134 29.54554523 |
| 2579 | | Rotation angle (degrees) 79.92830613 |
| 2580 | | Shift along axis -165.16783062 |
| 2581 | | |
| 2582 | | |
| 2583 | | > fitmap #1 inMap #2 |
| 2584 | | |
| 2585 | | Fit molecule 5zf6.pdb (#1) to map cryosparc_P1_J138_004_volume_map_sharp.mrc |
| 2586 | | (#2) using 8555 atoms |
| 2587 | | average map value = 0.1291, steps = 2000 |
| 2588 | | shifted from previous position = 0.427 |
| 2589 | | rotated from previous position = 0.28 degrees |
| 2590 | | atoms outside contour = 4695, contour level = 0.10328 |
| 2591 | | |
| 2592 | | Position of 5zf6.pdb (#1) relative to |
| 2593 | | cryosparc_P1_J138_004_volume_map_sharp.mrc (#2) coordinates: |
| 2594 | | Matrix rotation and translation |
| 2595 | | 0.95531248 0.12439245 -0.26815027 146.59108838 |
| 2596 | | 0.23788270 0.21497353 0.94720546 145.90157205 |
| 2597 | | 0.17547042 -0.96866552 0.17577609 183.80822404 |
| 2598 | | Axis -0.97260592 -0.22520729 0.05761416 |
| 2599 | | Axis point 0.00000000 174.33443147 29.64460255 |
| 2600 | | Rotation angle (degrees) 80.03590204 |
| 2601 | | Shift along axis -164.84350294 |
| 2602 | | |
| 2603 | | |
| 2604 | | > fitmap #1 inMap #2 |
| 2605 | | |
| 2606 | | Fit molecule 5zf6.pdb (#1) to map cryosparc_P1_J138_004_volume_map_sharp.mrc |
| 2607 | | (#2) using 8555 atoms |
| 2608 | | average map value = 0.129, steps = 2000 |
| 2609 | | shifted from previous position = 0.223 |
| 2610 | | rotated from previous position = 0.829 degrees |
| 2611 | | atoms outside contour = 4631, contour level = 0.10328 |
| 2612 | | |
| 2613 | | Position of 5zf6.pdb (#1) relative to |
| 2614 | | cryosparc_P1_J138_004_volume_map_sharp.mrc (#2) coordinates: |
| 2615 | | Matrix rotation and translation |
| 2616 | | 0.95523491 0.12627846 -0.26754441 146.55435334 |
| 2617 | | 0.23947844 0.20095469 0.94987751 145.96486274 |
| 2618 | | 0.17371337 -0.97142727 0.16171797 183.59795330 |
| 2619 | | Axis -0.97302339 -0.22347009 0.05732887 |
| 2620 | | Axis point 0.00000000 172.59699771 30.26934598 |
| 2621 | | Rotation angle (degrees) 80.85382484 |
| 2622 | | Shift along axis -164.69413167 |
| 2623 | | |
| 2624 | | |
| 2625 | | > select add #1 |
| 2626 | | |
| 2627 | | 8555 atoms, 8618 bonds, 1174 residues, 1 model selected |
| 2628 | | |
| 2629 | | > fitmap #1 inMap #2 |
| 2630 | | |
| 2631 | | Fit molecule 5zf6.pdb (#1) to map cryosparc_P1_J138_004_volume_map_sharp.mrc |
| 2632 | | (#2) using 8555 atoms |
| 2633 | | average map value = 0.1276, steps = 2000 |
| 2634 | | shifted from previous position = 0.578 |
| 2635 | | rotated from previous position = 0.903 degrees |
| 2636 | | atoms outside contour = 4673, contour level = 0.10328 |
| 2637 | | |
| 2638 | | Position of 5zf6.pdb (#1) relative to |
| 2639 | | cryosparc_P1_J138_004_volume_map_sharp.mrc (#2) coordinates: |
| 2640 | | Matrix rotation and translation |
| 2641 | | 0.95643333 0.12570810 -0.26350093 146.62391692 |
| 2642 | | 0.23300137 0.21516139 0.94837541 145.55544584 |
| 2643 | | 0.17591370 -0.96845394 0.17649737 184.00030683 |
| 2644 | | Axis -0.97326919 -0.22311257 0.05447811 |
| 2645 | | Axis point 0.00000000 174.18154237 29.52131391 |
| 2646 | | Rotation angle (degrees) 79.97685109 |
| 2647 | | Shift along axis -165.15580209 |
| 2648 | | |
| 2649 | | |
| 2650 | | > fitmap #1 inMap #2 |
| 2651 | | |
| 2652 | | Fit molecule 5zf6.pdb (#1) to map cryosparc_P1_J138_004_volume_map_sharp.mrc |
| 2653 | | (#2) using 8555 atoms |
| 2654 | | average map value = 0.1291, steps = 2000 |
| 2655 | | shifted from previous position = 0.363 |
| 2656 | | rotated from previous position = 0.352 degrees |
| 2657 | | atoms outside contour = 4685, contour level = 0.10328 |
| 2658 | | |
| 2659 | | Position of 5zf6.pdb (#1) relative to |
| 2660 | | cryosparc_P1_J138_004_volume_map_sharp.mrc (#2) coordinates: |
| 2661 | | Matrix rotation and translation |
| 2662 | | 0.95497719 0.12448000 -0.26930149 146.60547981 |
| 2663 | | 0.23896831 0.21519467 0.94688193 145.87389672 |
| 2664 | | 0.17582011 -0.96860517 0.17575923 183.82784665 |
| 2665 | | Axis -0.97239968 -0.22596660 0.05812015 |
| 2666 | | Axis point 0.00000000 174.33808153 29.77706094 |
| 2667 | | Rotation angle (degrees) 80.03971295 |
| 2668 | | Shift along axis -164.83764797 |
| 2669 | | |
| 2670 | | |
| 2671 | | > volume gaussian #1 sDev 2 |
| 2672 | | |
| 2673 | | [Repeated 1 time(s)] |
| 2674 | | |
| 2675 | | > hide #!2 models |
| 2676 | | |
| 2677 | | > select subtract #1 |
| 2678 | | |
| 2679 | | Nothing selected |
| 2680 | | |
| 2681 | | > select :260-320 |
| 2682 | | |
| 2683 | | 3024 atoms, 3078 bonds, 366 residues, 3 models selected |
| 2684 | | |
| 2685 | | > select add #3 |
| 2686 | | |
| 2687 | | 10571 atoms, 10670 bonds, 1418 residues, 3 models selected |
| 2688 | | |
| 2689 | | > select add #4 |
| 2690 | | |
| 2691 | | 18118 atoms, 18262 bonds, 2470 residues, 3 models selected |
| 2692 | | |
| 2693 | | > select subtract #4 |
| 2694 | | |
| 2695 | | 9563 atoms, 9644 bonds, 1296 residues, 2 models selected |
| 2696 | | |
| 2697 | | > select subtract #3 |
| 2698 | | |
| 2699 | | 1008 atoms, 1026 bonds, 122 residues, 1 model selected |
| 2700 | | |
| 2701 | | > select add #2 |
| 2702 | | |
| 2703 | | 1008 atoms, 1026 bonds, 122 residues, 3 models selected |
| 2704 | | |
| 2705 | | > show #!2 models |
| 2706 | | |
| 2707 | | > hide #!1 models |
| 2708 | | |
| 2709 | | > volume guassian #1 stdev 2 |
| 2710 | | |
| 2711 | | Expected a density maps specifier or a keyword |
| 2712 | | |
| 2713 | | > select subtract #2 |
| 2714 | | |
| 2715 | | 1008 atoms, 1026 bonds, 122 residues, 1 model selected |
| 2716 | | |
| 2717 | | > show #!1 models |
| 2718 | | |
| 2719 | | > select add #1 |
| 2720 | | |
| 2721 | | 8555 atoms, 8618 bonds, 1174 residues, 1 model selected |
| 2722 | | |
| 2723 | | > select subtract #1 |
| 2724 | | |
| 2725 | | Nothing selected |
| 2726 | | |
| 2727 | | > hide #!2 models |
| 2728 | | |
| 2729 | | > select :270-312 |
| 2730 | | |
| 2731 | | 2172 atoms, 2226 bonds, 258 residues, 3 models selected |
| 2732 | | |
| 2733 | | > select :270-320 |
| 2734 | | |
| 2735 | | 2568 atoms, 2622 bonds, 306 residues, 3 models selected |
| 2736 | | |
| 2737 | | > select add #2 |
| 2738 | | |
| 2739 | | 2568 atoms, 2622 bonds, 306 residues, 5 models selected |
| 2740 | | |
| 2741 | | > show #!2 models |
| 2742 | | |
| 2743 | | > select subtract #2 |
| 2744 | | |
| 2745 | | 2568 atoms, 2622 bonds, 306 residues, 3 models selected |
| 2746 | | |
| 2747 | | > fitmap #1 inMap #5 |
| 2748 | | |
| 2749 | | Fit molecule 5zf6.pdb (#1) to map 5zf6.pdb map 4 (#5) using 8555 atoms |
| 2750 | | average map value = 0.6588, steps = 44 |
| 2751 | | shifted from previous position = 0.251 |
| 2752 | | rotated from previous position = 0.917 degrees |
| 2753 | | atoms outside contour = 0, contour level = 0.10406 |
| 2754 | | |
| 2755 | | Position of 5zf6.pdb (#1) relative to 5zf6.pdb map 4 (#5) coordinates: |
| 2756 | | Matrix rotation and translation |
| 2757 | | 0.99999998 -0.00021839 -0.00002624 -0.00561747 |
| 2758 | | 0.00021838 0.99999996 -0.00015664 -0.02705654 |
| 2759 | | 0.00002627 0.00015663 0.99999999 -0.00142914 |
| 2760 | | Axis 0.58007012 -0.09724114 0.80874150 |
| 2761 | | Axis point 124.68665962 -20.99976529 -0.00000000 |
| 2762 | | Rotation angle (degrees) 0.01547148 |
| 2763 | | Shift along axis -0.00178332 |
| 2764 | | |
| 2765 | | |
| 2766 | | > fitmap #1 inMap #2 |
| 2767 | | |
| 2768 | | Fit molecule 5zf6.pdb (#1) to map cryosparc_P1_J138_004_volume_map_sharp.mrc |
| 2769 | | (#2) using 8555 atoms |
| 2770 | | average map value = 0.1297, steps = 192 |
| 2771 | | shifted from previous position = 0.223 |
| 2772 | | rotated from previous position = 0.497 degrees |
| 2773 | | atoms outside contour = 4641, contour level = 0.10328 |
| 2774 | | |
| 2775 | | Position of 5zf6.pdb (#1) relative to |
| 2776 | | cryosparc_P1_J138_004_volume_map_sharp.mrc (#2) coordinates: |
| 2777 | | Matrix rotation and translation |
| 2778 | | 0.95551911 0.12624268 -0.26654456 146.57951723 |
| 2779 | | 0.23725555 0.20782832 0.94895584 145.98336518 |
| 2780 | | 0.17519424 -0.96998462 0.16863216 183.82012764 |
| 2781 | | Axis -0.97296776 -0.22397652 0.05628729 |
| 2782 | | Axis point 0.00000000 173.45562784 29.89819058 |
| 2783 | | Rotation angle (degrees) 80.44526139 |
| 2784 | | Shift along axis -164.96725473 |
| 2785 | | |
| 2786 | | |
| 2787 | | > fitmap #1 inMap #2 moveWholeMolecules false |
| 2788 | | |
| 2789 | | Fit molecule 5zf6.pdb (#1) to map cryosparc_P1_J138_004_volume_map_sharp.mrc |
| 2790 | | (#2) using 8555 atoms |
| 2791 | | average map value = 0.1297, steps = 116 |
| 2792 | | shifted from previous position = 0.00753 |
| 2793 | | rotated from previous position = 0.0169 degrees |
| 2794 | | atoms outside contour = 4645, contour level = 0.10328 |
| 2795 | | |
| 2796 | | |
| 2797 | | > fitmap #1 inMap #2 moveWholeMolecules false |
| 2798 | | |
| 2799 | | Fit molecule 5zf6.pdb (#1) to map cryosparc_P1_J138_004_volume_map_sharp.mrc |
| 2800 | | (#2) using 8555 atoms |
| 2801 | | average map value = 0.1297, steps = 340 |
| 2802 | | shifted from previous position = 0.00951 |
| 2803 | | rotated from previous position = 0.0154 degrees |
| 2804 | | atoms outside contour = 4645, contour level = 0.10328 |
| 2805 | | |
| 2806 | | |
| 2807 | | > fitmap #1 inMap #5 moveWholeMolecules false |
| 2808 | | |
| 2809 | | Fit molecule 5zf6.pdb (#1) to map 5zf6.pdb map 4 (#5) using 8555 atoms |
| 2810 | | average map value = 0.6588, steps = 44 |
| 2811 | | shifted from previous position = 0.233 |
| 2812 | | rotated from previous position = 0.48 degrees |
| 2813 | | atoms outside contour = 0, contour level = 0.10406 |
| 2814 | | |
| 2815 | | |
| 2816 | | > select add #1 |
| 2817 | | |
| 2818 | | 10267 atoms, 10366 bonds, 1378 residues, 3 models selected |
| 2819 | | |
| 2820 | | > select subtract #1 |
| 2821 | | |
| 2822 | | 1712 atoms, 1748 bonds, 204 residues, 2 models selected |
| 2823 | | |
| 2824 | | > volume #2 color #b2b2b268 |
| 2825 | | |
| 2826 | | > volume #2 color #b2b2b269 |
| 2827 | | |
| 2828 | | > ui tool show "Show Sequence Viewer" |
| 2829 | | |
| 2830 | | > sequence chain #1/B |
| 2831 | | |
| 2832 | | Destroying pre-existing alignment with identifier 1/B |
| 2833 | | Alignment identifier is 1/B |
| 2834 | | |
| 2835 | | > select #1/B:268-269 |
| 2836 | | |
| 2837 | | 14 atoms, 13 bonds, 2 residues, 1 model selected |
| 2838 | | |
| 2839 | | > select #1/B:268-288 |
| 2840 | | |
| 2841 | | 174 atoms, 179 bonds, 21 residues, 1 model selected |
| 2842 | | |
| 2843 | | > select |
| 2844 | | > #1/B:125-142,164-180,207-212,222-224,237-267,280-299,305-328,332-356,450-462,482-499,534-540,567-593,622-636,653-666 |
| 2845 | | |
| 2846 | | 1850 atoms, 1864 bonds, 238 residues, 1 model selected |
| 2847 | | |
| 2848 | | > select clear |
| 2849 | | |
| 2850 | | > select #1/B:304 |
| 2851 | | |
| 2852 | | 10 atoms, 10 bonds, 1 residue, 1 model selected |
| 2853 | | |
| 2854 | | > select #1/B:304-305 |
| 2855 | | |
| 2856 | | 18 atoms, 18 bonds, 2 residues, 1 model selected |
| 2857 | | |
| 2858 | | > select #1/B:304 |
| 2859 | | |
| 2860 | | 10 atoms, 10 bonds, 1 residue, 1 model selected |
| 2861 | | |
| 2862 | | > select #1/B:304-322 |
| 2863 | | |
| 2864 | | 157 atoms, 157 bonds, 19 residues, 1 model selected |
| 2865 | | |
| 2866 | | > select #1/B:331-332 |
| 2867 | | |
| 2868 | | 13 atoms, 12 bonds, 2 residues, 1 model selected |
| 2869 | | |
| 2870 | | > select #1/B:302-332 |
| 2871 | | |
| 2872 | | 262 atoms, 265 bonds, 31 residues, 1 model selected |
| 2873 | | |
| 2874 | | > fitmap #1 inMap #2 moveWholeMolecules false |
| 2875 | | |
| 2876 | | Fit molecule 5zf6.pdb (#1) to map cryosparc_P1_J138_004_volume_map_sharp.mrc |
| 2877 | | (#2) using 8555 atoms |
| 2878 | | average map value = 0.1297, steps = 232 |
| 2879 | | shifted from previous position = 0.214 |
| 2880 | | rotated from previous position = 0.47 degrees |
| 2881 | | atoms outside contour = 4639, contour level = 0.10328 |
| 2882 | | |
| 2883 | | |
| 2884 | | > fitmap #1 inMap #2 moveWholeMolecules false |
| 2885 | | |
| 2886 | | Fit molecule 5zf6.pdb (#1) to map cryosparc_P1_J138_004_volume_map_sharp.mrc |
| 2887 | | (#2) using 8555 atoms |
| 2888 | | average map value = 0.1291, steps = 2000 |
| 2889 | | shifted from previous position = 0.0958 |
| 2890 | | rotated from previous position = 0.454 degrees |
| 2891 | | atoms outside contour = 4687, contour level = 0.10328 |
| 2892 | | |
| 2893 | | |
| 2894 | | > fitmap #1 inMap #2 moveWholeMolecules false |
| 2895 | | |
| 2896 | | Fit molecule 5zf6.pdb (#1) to map cryosparc_P1_J138_004_volume_map_sharp.mrc |
| 2897 | | (#2) using 8555 atoms |
| 2898 | | average map value = 0.129, steps = 2000 |
| 2899 | | shifted from previous position = 0.25 |
| 2900 | | rotated from previous position = 0.875 degrees |
| 2901 | | atoms outside contour = 4630, contour level = 0.10328 |
| 2902 | | |
| 2903 | | |
| 2904 | | > fitmap #1 inMap #2 moveWholeMolecules false |
| 2905 | | |
| 2906 | | Fit molecule 5zf6.pdb (#1) to map cryosparc_P1_J138_004_volume_map_sharp.mrc |
| 2907 | | (#2) using 8555 atoms |
| 2908 | | average map value = 0.1277, steps = 2000 |
| 2909 | | shifted from previous position = 0.604 |
| 2910 | | rotated from previous position = 0.954 degrees |
| 2911 | | atoms outside contour = 4674, contour level = 0.10328 |
| 2912 | | |
| 2913 | | |
| 2914 | | > fitmap #1 inMap #2 moveWholeMolecules false |
| 2915 | | |
| 2916 | | Fit molecule 5zf6.pdb (#1) to map cryosparc_P1_J138_004_volume_map_sharp.mrc |
| 2917 | | (#2) using 8555 atoms |
| 2918 | | average map value = 0.1291, steps = 2000 |
| 2919 | | shifted from previous position = 0.392 |
| 2920 | | rotated from previous position = 0.273 degrees |
| 2921 | | atoms outside contour = 4688, contour level = 0.10328 |
| 2922 | | |
| 2923 | | |
| 2924 | | > ui mousemode right "rotate selected models" |
| 2925 | | |
| 2926 | | > select add #1 |
| 2927 | | |
| 2928 | | 8555 atoms, 8618 bonds, 1174 residues, 1 model selected |
| 2929 | | |
| 2930 | | > view matrix models |
| 2931 | | > #1,0.9936,-0.094139,-0.062377,209.56,0.083684,0.24289,0.96644,190.72,-0.075829,-0.96548,0.24921,212.87 |
| 2932 | | |
| 2933 | | > fitmap #1 inMap #2 moveWholeMolecules false |
| 2934 | | |
| 2935 | | Fit molecule 5zf6.pdb (#1) to map cryosparc_P1_J138_004_volume_map_sharp.mrc |
| 2936 | | (#2) using 8555 atoms |
| 2937 | | average map value = 0.1297, steps = 408 |
| 2938 | | shifted from previous position = 0.0525 |
| 2939 | | rotated from previous position = 7.42 degrees |
| 2940 | | atoms outside contour = 4643, contour level = 0.10328 |
| 2941 | | |
| 2942 | | |
| 2943 | | > ui tool show "Show Sequence Viewer" |
| 2944 | | |
| 2945 | | > sequence chain #1/B |
| 2946 | | |
| 2947 | | Alignment identifier is 1/B |
| 2948 | | |
| 2949 | | > select :200-320 |
| 2950 | | |
| 2951 | | 5772 atoms, 5862 bonds, 726 residues, 3 models selected |
| 2952 | | |
| 2953 | | > fitmap #1 inMap #2 moveWholeMolecules false |
| 2954 | | |
| 2955 | | Fit molecule 5zf6.pdb (#1) to map cryosparc_P1_J138_004_volume_map_sharp.mrc |
| 2956 | | (#2) using 8555 atoms |
| 2957 | | average map value = 0.1289, steps = 2000 |
| 2958 | | shifted from previous position = 0.251 |
| 2959 | | rotated from previous position = 0.502 degrees |
| 2960 | | atoms outside contour = 4632, contour level = 0.10328 |
| 2961 | | |
| 2962 | | |
| 2963 | | > fitmap #1 inMap #5 moveWholeMolecules false |
| 2964 | | |
| 2965 | | Fit molecule 5zf6.pdb (#1) to map 5zf6.pdb map 4 (#5) using 8555 atoms |
| 2966 | | average map value = 0.6589, steps = 40 |
| 2967 | | shifted from previous position = 0.0131 |
| 2968 | | rotated from previous position = 0.0174 degrees |
| 2969 | | atoms outside contour = 0, contour level = 0.10406 |
| 2970 | | |
| 2971 | | |
| 2972 | | > fitmap #1 inMap #5 moveWholeMolecules false |
| 2973 | | |
| 2974 | | Fit molecule 5zf6.pdb (#1) to map 5zf6.pdb map 4 (#5) using 8555 atoms |
| 2975 | | average map value = 0.6588, steps = 40 |
| 2976 | | shifted from previous position = 0.0121 |
| 2977 | | rotated from previous position = 0.00956 degrees |
| 2978 | | atoms outside contour = 0, contour level = 0.10406 |
| 2979 | | |
| 2980 | | |
| 2981 | | > fitmap #1 inMap #5 moveWholeMolecules false |
| 2982 | | |
| 2983 | | Fit molecule 5zf6.pdb (#1) to map 5zf6.pdb map 4 (#5) using 8555 atoms |
| 2984 | | average map value = 0.6589, steps = 40 |
| 2985 | | shifted from previous position = 0.0371 |
| 2986 | | rotated from previous position = 0.0128 degrees |
| 2987 | | atoms outside contour = 0, contour level = 0.10406 |
| 2988 | | |
| 2989 | | |
| 2990 | | > fitmap #4 inMap #2 moveWholeMolecules false |
| 2991 | | |
| 2992 | | Fit molecule 5zf6.pdb (#4) to map cryosparc_P1_J138_004_volume_map_sharp.mrc |
| 2993 | | (#2) using 8555 atoms |
| 2994 | | average map value = 0.000603, steps = 96 |
| 2995 | | shifted from previous position = 1.87 |
| 2996 | | rotated from previous position = 3.83 degrees |
| 2997 | | atoms outside contour = 8554, contour level = 0.10328 |
| 2998 | | |
| 2999 | | |
| 3000 | | > fitmap #4 inMap #2 moveWholeMolecules false |
| 3001 | | |
| 3002 | | Fit molecule 5zf6.pdb (#4) to map cryosparc_P1_J138_004_volume_map_sharp.mrc |
| 3003 | | (#2) using 8555 atoms |
| 3004 | | average map value = 0.0006029, steps = 44 |
| 3005 | | shifted from previous position = 0.00257 |
| 3006 | | rotated from previous position = 0.00598 degrees |
| 3007 | | atoms outside contour = 8554, contour level = 0.10328 |
| 3008 | | |
| 3009 | | |
| 3010 | | > select add #3 |
| 3011 | | |
| 3012 | | 12403 atoms, 12526 bonds, 1658 residues, 3 models selected |
| 3013 | | |
| 3014 | | > select add #4 |
| 3015 | | |
| 3016 | | 19034 atoms, 19190 bonds, 2590 residues, 3 models selected |
| 3017 | | |
| 3018 | | > select subtract #4 |
| 3019 | | |
| 3020 | | 10479 atoms, 10572 bonds, 1416 residues, 2 models selected |
| 3021 | | |
| 3022 | | > select subtract #3 |
| 3023 | | |
| 3024 | | 1924 atoms, 1954 bonds, 242 residues, 1 model selected |
| 3025 | | |
| 3026 | | > fitmap #4 inMap #2 moveWholeMolecules false |
| 3027 | | |
| 3028 | | Fit molecule 5zf6.pdb (#4) to map cryosparc_P1_J138_004_volume_map_sharp.mrc |
| 3029 | | (#2) using 8555 atoms |
| 3030 | | average map value = 0.0006029, steps = 48 |
| 3031 | | shifted from previous position = 0.00238 |
| 3032 | | rotated from previous position = 0.0048 degrees |
| 3033 | | atoms outside contour = 8554, contour level = 0.10328 |
| 3034 | | |
| 3035 | | |
| 3036 | | > select add #1 |
| 3037 | | |
| 3038 | | 8555 atoms, 8618 bonds, 1174 residues, 1 model selected |
| 3039 | | |
| 3040 | | > select subtract #1 |
| 3041 | | |
| 3042 | | Nothing selected |
| 3043 | | |
| 3044 | | > open /Users/madhurikanavalli/Downloads/hPNP_dimer_5zf6_fit_tetramer1.pdb |
| 3045 | | |
| 3046 | | hPNP_dimer_5zf6_fit_tetramer1.pdb title: |
| 3047 | | Crystal structure of the dimeric human pnpase [more info...] |
| 3048 | | |
| 3049 | | Chain information for hPNP_dimer_5zf6_fit_tetramer1.pdb #6 |
| 3050 | | --- |
| 3051 | | Chain | Description | UniProt |
| 3052 | | A B | polyribonucleotide nucleotidyltransferase 1, mitochondrial | PNPT1_HUMAN 46-669 |
| 3053 | | |
| 3054 | | |
| 3055 | | > hide #!1 models |
| 3056 | | |
| 3057 | | > select add #2 |
| 3058 | | |
| 3059 | | 2 models selected |
| 3060 | | |
| 3061 | | > select subtract #2 |
| 3062 | | |
| 3063 | | Nothing selected |
| 3064 | | |
| 3065 | | > select add #6 |
| 3066 | | |
| 3067 | | 8555 atoms, 8618 bonds, 1174 residues, 1 model selected |
| 3068 | | |
| 3069 | | > select add #2 |
| 3070 | | |
| 3071 | | 8555 atoms, 8618 bonds, 1174 residues, 3 models selected |
| 3072 | | |
| 3073 | | > fitmap #4 inMap #2 |
| 3074 | | |
| 3075 | | Fit molecule 5zf6.pdb (#4) to map cryosparc_P1_J138_004_volume_map_sharp.mrc |
| 3076 | | (#2) using 8555 atoms |
| 3077 | | average map value = 0.0006029, steps = 44 |
| 3078 | | shifted from previous position = 0.00473 |
| 3079 | | rotated from previous position = 0.0042 degrees |
| 3080 | | atoms outside contour = 8554, contour level = 0.10328 |
| 3081 | | |
| 3082 | | Position of 5zf6.pdb (#4) relative to |
| 3083 | | cryosparc_P1_J138_004_volume_map_sharp.mrc (#2) coordinates: |
| 3084 | | Matrix rotation and translation |
| 3085 | | 0.63645764 -0.77048569 -0.03568582 78.12947023 |
| 3086 | | 0.21006805 0.12863727 0.96918722 38.53015783 |
| 3087 | | -0.74215436 -0.62434307 0.24372659 302.42254591 |
| 3088 | | Axis -0.79677289 0.35323770 0.49028163 |
| 3089 | | Axis point -0.00000000 196.41371757 173.14599918 |
| 3090 | | Rotation angle (degrees) 89.74728167 |
| 3091 | | Shift along axis 99.63108047 |
| 3092 | | |
| 3093 | | |
| 3094 | | > fitmap #4 inMap #2 |
| 3095 | | |
| 3096 | | Fit molecule 5zf6.pdb (#4) to map cryosparc_P1_J138_004_volume_map_sharp.mrc |
| 3097 | | (#2) using 8555 atoms |
| 3098 | | average map value = 0.0006032, steps = 48 |
| 3099 | | shifted from previous position = 0.00496 |
| 3100 | | rotated from previous position = 0.0115 degrees |
| 3101 | | atoms outside contour = 8554, contour level = 0.10328 |
| 3102 | | |
| 3103 | | Position of 5zf6.pdb (#4) relative to |
| 3104 | | cryosparc_P1_J138_004_volume_map_sharp.mrc (#2) coordinates: |
| 3105 | | Matrix rotation and translation |
| 3106 | | 0.63656824 -0.77038796 -0.03582278 78.12779013 |
| 3107 | | 0.21013537 0.12856732 0.96918191 38.52558497 |
| 3108 | | -0.74204044 -0.62447805 0.24372763 302.41095664 |
| 3109 | | Axis -0.79683780 0.35311230 0.49026648 |
| 3110 | | Axis point 0.00000000 196.39377529 173.12757133 |
| 3111 | | Rotation angle (degrees) 89.74608769 |
| 3112 | | Shift along axis 99.61063610 |
| 3113 | | |
| 3114 | | |
| 3115 | | > select subtract #2 |
| 3116 | | |
| 3117 | | 8555 atoms, 8618 bonds, 1174 residues, 1 model selected |
| 3118 | | |
| 3119 | | > fitmap #4 inMap #2 |
| 3120 | | |
| 3121 | | Fit molecule 5zf6.pdb (#4) to map cryosparc_P1_J138_004_volume_map_sharp.mrc |
| 3122 | | (#2) using 8555 atoms |
| 3123 | | average map value = 0.0006032, steps = 48 |
| 3124 | | shifted from previous position = 0.0201 |
| 3125 | | rotated from previous position = 0.0227 degrees |
| 3126 | | atoms outside contour = 8554, contour level = 0.10328 |
| 3127 | | |
| 3128 | | Position of 5zf6.pdb (#4) relative to |
| 3129 | | cryosparc_P1_J138_004_volume_map_sharp.mrc (#2) coordinates: |
| 3130 | | Matrix rotation and translation |
| 3131 | | 0.63632725 -0.77059781 -0.03559006 78.13063330 |
| 3132 | | 0.21006324 0.12869982 0.96917996 38.51852128 |
| 3133 | | -0.74226752 -0.62419178 0.24376948 302.43743598 |
| 3134 | | Axis -0.79669358 0.35334215 0.49033527 |
| 3135 | | Axis point 0.00000000 196.42606613 173.17892989 |
| 3136 | | Rotation angle (degrees) 89.74799671 |
| 3137 | | Shift along axis 99.65978425 |
| 3138 | | |
| 3139 | | |
| 3140 | | > fitmap #6 inMap #2 |
| 3141 | | |
| 3142 | | Fit molecule hPNP_dimer_5zf6_fit_tetramer1.pdb (#6) to map |
| 3143 | | cryosparc_P1_J138_004_volume_map_sharp.mrc (#2) using 8555 atoms |
| 3144 | | average map value = 0.1265, steps = 2000 |
| 3145 | | shifted from previous position = 26.4 |
| 3146 | | rotated from previous position = 14.9 degrees |
| 3147 | | atoms outside contour = 4568, contour level = 0.10328 |
| 3148 | | |
| 3149 | | Position of hPNP_dimer_5zf6_fit_tetramer1.pdb (#6) relative to |
| 3150 | | cryosparc_P1_J138_004_volume_map_sharp.mrc (#2) coordinates: |
| 3151 | | Matrix rotation and translation |
| 3152 | | 0.99998715 0.00501254 -0.00076596 -38.39780829 |
| 3153 | | -0.00499658 0.99979573 0.01958429 -41.08711100 |
| 3154 | | 0.00086397 -0.01958021 0.99980792 -27.19225070 |
| 3155 | | Axis -0.96807371 -0.04028867 -0.24740679 |
| 3156 | | Axis point 0.00000000 -832.72852867 1995.56199881 |
| 3157 | | Rotation angle (degrees) 1.15906129 |
| 3158 | | Shift along axis 45.55480119 |
| 3159 | | |
| 3160 | | |
| 3161 | | > fitmap #6 inMap #5 |
| 3162 | | |
| 3163 | | Fit molecule hPNP_dimer_5zf6_fit_tetramer1.pdb (#6) to map 5zf6.pdb map 4 (#5) |
| 3164 | | using 8555 atoms |
| 3165 | | average map value = 0.6589, steps = 80 |
| 3166 | | shifted from previous position = 6.92 |
| 3167 | | rotated from previous position = 21.3 degrees |
| 3168 | | atoms outside contour = 0, contour level = 0.10406 |
| 3169 | | |
| 3170 | | Position of hPNP_dimer_5zf6_fit_tetramer1.pdb (#6) relative to 5zf6.pdb map 4 |
| 3171 | | (#5) coordinates: |
| 3172 | | Matrix rotation and translation |
| 3173 | | 0.97042337 0.22451382 -0.08872453 -205.64095764 |
| 3174 | | -0.06918024 -0.09348358 -0.99321444 243.81703053 |
| 3175 | | -0.23128466 0.96997648 -0.07518673 -124.63683134 |
| 3176 | | Axis 0.98645360 0.07163285 -0.14757381 |
| 3177 | | Axis point 0.00000000 197.87770362 39.67267018 |
| 3178 | | Rotation angle (degrees) 95.68869835 |
| 3179 | | Shift along axis -166.99682276 |
| 3180 | | |
| 3181 | | |
| 3182 | | > select subtract #6 |
| 3183 | | |
| 3184 | | Nothing selected |
| 3185 | | |
| 3186 | | > hide #!6 models |
| 3187 | | |
| 3188 | | > color #6 #db5f27ff |
| 3189 | | |
| 3190 | | > color #6 #db7553ff |
| 3191 | | |
| 3192 | | > color #6 #db9a8cff |
| 3193 | | |
| 3194 | | > color #6 #db7a33ff |
| 3195 | | |
| 3196 | | > color #6 #db5b31ff |
| 3197 | | |
| 3198 | | > color #6 #db4122ff |
| 3199 | | |
| 3200 | | > show #!6 models |
| 3201 | | |
| 3202 | | > ui tool show "Show Sequence Viewer" |
| 3203 | | |
| 3204 | | > sequence chain #6/B |
| 3205 | | |
| 3206 | | Alignment identifier is 6/B |
| 3207 | | |
| 3208 | | > hide #!2 models |
| 3209 | | |
| 3210 | | > select #6/B:293 |
| 3211 | | |
| 3212 | | 9 atoms, 8 bonds, 1 residue, 1 model selected |
| 3213 | | |
| 3214 | | > select #6/B:293-363 |
| 3215 | | |
| 3216 | | 599 atoms, 609 bonds, 71 residues, 1 model selected |
| 3217 | | |
| 3218 | | > select #6/B:270 |
| 3219 | | |
| 3220 | | 9 atoms, 8 bonds, 1 residue, 1 model selected |
| 3221 | | |
| 3222 | | > select #6/B:270-369 |
| 3223 | | |
| 3224 | | 837 atoms, 853 bonds, 100 residues, 1 model selected |
| 3225 | | |
| 3226 | | > select add #2 |
| 3227 | | |
| 3228 | | 837 atoms, 853 bonds, 100 residues, 3 models selected |
| 3229 | | |
| 3230 | | > show #!2 models |
| 3231 | | |
| 3232 | | > fitmap #6 inMap #5 moveWholeMolecules false |
| 3233 | | |
| 3234 | | Fit molecule hPNP_dimer_5zf6_fit_tetramer1.pdb (#6) to map 5zf6.pdb map 4 (#5) |
| 3235 | | using 8555 atoms |
| 3236 | | average map value = 0.6589, steps = 40 |
| 3237 | | shifted from previous position = 0.0331 |
| 3238 | | rotated from previous position = 0.0118 degrees |
| 3239 | | atoms outside contour = 0, contour level = 0.10406 |
| 3240 | | |
| 3241 | | |
| 3242 | | > fitmap #6 inMap #5 moveWholeMolecules false |
| 3243 | | |
| 3244 | | Fit molecule hPNP_dimer_5zf6_fit_tetramer1.pdb (#6) to map 5zf6.pdb map 4 (#5) |
| 3245 | | using 8555 atoms |
| 3246 | | average map value = 0.6589, steps = 40 |
| 3247 | | shifted from previous position = 0.0336 |
| 3248 | | rotated from previous position = 0.00485 degrees |
| 3249 | | atoms outside contour = 0, contour level = 0.10406 |
| 3250 | | |
| 3251 | | |
| 3252 | | > fitmap #6 inMap #5 moveWholeMolecules false |
| 3253 | | |
| 3254 | | Fit molecule hPNP_dimer_5zf6_fit_tetramer1.pdb (#6) to map 5zf6.pdb map 4 (#5) |
| 3255 | | using 8555 atoms |
| 3256 | | average map value = 0.6589, steps = 40 |
| 3257 | | shifted from previous position = 0.03 |
| 3258 | | rotated from previous position = 0.0369 degrees |
| 3259 | | atoms outside contour = 0, contour level = 0.10406 |
| 3260 | | |
| 3261 | | |
| 3262 | | > fitmap #6 inMap #5 moveWholeMolecules false |
| 3263 | | |
| 3264 | | Fit molecule hPNP_dimer_5zf6_fit_tetramer1.pdb (#6) to map 5zf6.pdb map 4 (#5) |
| 3265 | | using 8555 atoms |
| 3266 | | average map value = 0.6589, steps = 40 |
| 3267 | | shifted from previous position = 0.00753 |
| 3268 | | rotated from previous position = 0.00808 degrees |
| 3269 | | atoms outside contour = 0, contour level = 0.10406 |
| 3270 | | |
| 3271 | | |
| 3272 | | > fitmap #6 inMap #5 moveWholeMolecules false |
| 3273 | | |
| 3274 | | Fit molecule hPNP_dimer_5zf6_fit_tetramer1.pdb (#6) to map 5zf6.pdb map 4 (#5) |
| 3275 | | using 8555 atoms |
| 3276 | | average map value = 0.6588, steps = 44 |
| 3277 | | shifted from previous position = 0.0101 |
| 3278 | | rotated from previous position = 0.0526 degrees |
| 3279 | | atoms outside contour = 0, contour level = 0.10406 |
| 3280 | | |
| 3281 | | |
| 3282 | | > fitmap #6 inMap #5 moveWholeMolecules false |
| 3283 | | |
| 3284 | | Fit molecule hPNP_dimer_5zf6_fit_tetramer1.pdb (#6) to map 5zf6.pdb map 4 (#5) |
| 3285 | | using 8555 atoms |
| 3286 | | average map value = 0.6589, steps = 40 |
| 3287 | | shifted from previous position = 0.0172 |
| 3288 | | rotated from previous position = 0.00948 degrees |
| 3289 | | atoms outside contour = 0, contour level = 0.10406 |
| 3290 | | |
| 3291 | | |
| 3292 | | > fitmap #6 inMap #5 moveWholeMolecules false |
| 3293 | | |
| 3294 | | Fit molecule hPNP_dimer_5zf6_fit_tetramer1.pdb (#6) to map 5zf6.pdb map 4 (#5) |
| 3295 | | using 8555 atoms |
| 3296 | | average map value = 0.6589, steps = 40 |
| 3297 | | shifted from previous position = 0.0295 |
| 3298 | | rotated from previous position = 0.0068 degrees |
| 3299 | | atoms outside contour = 0, contour level = 0.10406 |
| 3300 | | |
| 3301 | | |
| 3302 | | > fitmap #6 inMap #5 moveWholeMolecules false |
| 3303 | | |
| 3304 | | Fit molecule hPNP_dimer_5zf6_fit_tetramer1.pdb (#6) to map 5zf6.pdb map 4 (#5) |
| 3305 | | using 8555 atoms |
| 3306 | | average map value = 0.6589, steps = 40 |
| 3307 | | shifted from previous position = 0.0301 |
| 3308 | | rotated from previous position = 0.033 degrees |
| 3309 | | atoms outside contour = 0, contour level = 0.10406 |
| 3310 | | |
| 3311 | | |
| 3312 | | > fitmap #6 inMap #5 moveWholeMolecules false |
| 3313 | | |
| 3314 | | Fit molecule hPNP_dimer_5zf6_fit_tetramer1.pdb (#6) to map 5zf6.pdb map 4 (#5) |
| 3315 | | using 8555 atoms |
| 3316 | | average map value = 0.6589, steps = 40 |
| 3317 | | shifted from previous position = 0.00827 |
| 3318 | | rotated from previous position = 0.00575 degrees |
| 3319 | | atoms outside contour = 0, contour level = 0.10406 |
| 3320 | | |
| 3321 | | |
| 3322 | | > fitmap #6 inMap #5 moveWholeMolecules false |
| 3323 | | |
| 3324 | | Fit molecule hPNP_dimer_5zf6_fit_tetramer1.pdb (#6) to map 5zf6.pdb map 4 (#5) |
| 3325 | | using 8555 atoms |
| 3326 | | average map value = 0.6588, steps = 44 |
| 3327 | | shifted from previous position = 0.0134 |
| 3328 | | rotated from previous position = 0.0519 degrees |
| 3329 | | atoms outside contour = 0, contour level = 0.10406 |
| 3330 | | |
| 3331 | | |
| 3332 | | > fitmap #6 inMap #5 moveWholeMolecules false |
| 3333 | | |
| 3334 | | Fit molecule hPNP_dimer_5zf6_fit_tetramer1.pdb (#6) to map 5zf6.pdb map 4 (#5) |
| 3335 | | using 8555 atoms |
| 3336 | | average map value = 0.6589, steps = 36 |
| 3337 | | shifted from previous position = 0.0421 |
| 3338 | | rotated from previous position = 0.0115 degrees |
| 3339 | | atoms outside contour = 0, contour level = 0.10406 |
| 3340 | | |
| 3341 | | |
| 3342 | | > fitmap #6 inMap #5 moveWholeMolecules false |
| 3343 | | |
| 3344 | | Fit molecule hPNP_dimer_5zf6_fit_tetramer1.pdb (#6) to map 5zf6.pdb map 4 (#5) |
| 3345 | | using 8555 atoms |
| 3346 | | average map value = 0.6589, steps = 40 |
| 3347 | | shifted from previous position = 0.033 |
| 3348 | | rotated from previous position = 0.048 degrees |
| 3349 | | atoms outside contour = 0, contour level = 0.10406 |
| 3350 | | |
| 3351 | | |
| 3352 | | > fitmap #6 inMap #5 moveWholeMolecules false |
| 3353 | | |
| 3354 | | Fit molecule hPNP_dimer_5zf6_fit_tetramer1.pdb (#6) to map 5zf6.pdb map 4 (#5) |
| 3355 | | using 8555 atoms |
| 3356 | | average map value = 0.6589, steps = 40 |
| 3357 | | shifted from previous position = 0.00797 |
| 3358 | | rotated from previous position = 0.0109 degrees |
| 3359 | | atoms outside contour = 0, contour level = 0.10406 |
| 3360 | | |
| 3361 | | |
| 3362 | | > fitmap #6 inMap #5 moveWholeMolecules false |
| 3363 | | |
| 3364 | | Fit molecule hPNP_dimer_5zf6_fit_tetramer1.pdb (#6) to map 5zf6.pdb map 4 (#5) |
| 3365 | | using 8555 atoms |
| 3366 | | average map value = 0.6588, steps = 44 |
| 3367 | | shifted from previous position = 0.0483 |
| 3368 | | rotated from previous position = 0.047 degrees |
| 3369 | | atoms outside contour = 0, contour level = 0.10406 |
| 3370 | | |
| 3371 | | |
| 3372 | | > select subtract #2 |
| 3373 | | |
| 3374 | | 837 atoms, 853 bonds, 100 residues, 1 model selected |
| 3375 | | |
| 3376 | | > fitmap #6 inMap #5 moveWholeMolecules false |
| 3377 | | |
| 3378 | | Fit molecule hPNP_dimer_5zf6_fit_tetramer1.pdb (#6) to map 5zf6.pdb map 4 (#5) |
| 3379 | | using 8555 atoms |
| 3380 | | average map value = 0.6588, steps = 28 |
| 3381 | | shifted from previous position = 0.0141 |
| 3382 | | rotated from previous position = 0.00276 degrees |
| 3383 | | atoms outside contour = 0, contour level = 0.10406 |
| 3384 | | |
| 3385 | | |
| 3386 | | > select #6/B:297-300 |
| 3387 | | |
| 3388 | | 30 atoms, 30 bonds, 4 residues, 1 model selected |
| 3389 | | |
| 3390 | | > select #6/B:235-300 |
| 3391 | | |
| 3392 | | 536 atoms, 546 bonds, 66 residues, 1 model selected |
| 3393 | | |
| 3394 | | > fitmap #6 inMap #2 moveWholeMolecules false |
| 3395 | | |
| 3396 | | Fit molecule hPNP_dimer_5zf6_fit_tetramer1.pdb (#6) to map |
| 3397 | | cryosparc_P1_J138_004_volume_map_sharp.mrc (#2) using 8555 atoms |
| 3398 | | average map value = 0.1275, steps = 2000 |
| 3399 | | shifted from previous position = 0.591 |
| 3400 | | rotated from previous position = 0.862 degrees |
| 3401 | | atoms outside contour = 4676, contour level = 0.10328 |
| 3402 | | |
| 3403 | | |
| 3404 | | > select #6/B:213 |
| 3405 | | |
| 3406 | | 7 atoms, 6 bonds, 1 residue, 1 model selected |
| 3407 | | |
| 3408 | | > select #6/B:213-306 |
| 3409 | | |
| 3410 | | 747 atoms, 760 bonds, 94 residues, 1 model selected |
| 3411 | | |
| 3412 | | > fitmap #6 inMap #2 moveWholeMolecules false |
| 3413 | | |
| 3414 | | Fit molecule hPNP_dimer_5zf6_fit_tetramer1.pdb (#6) to map |
| 3415 | | cryosparc_P1_J138_004_volume_map_sharp.mrc (#2) using 8555 atoms |
| 3416 | | average map value = 0.129, steps = 2000 |
| 3417 | | shifted from previous position = 0.31 |
| 3418 | | rotated from previous position = 0.339 degrees |
| 3419 | | atoms outside contour = 4685, contour level = 0.10328 |
| 3420 | | |
| 3421 | | |
| 3422 | | > fitmap #6 inMap #2 moveWholeMolecules false |
| 3423 | | |
| 3424 | | Fit molecule hPNP_dimer_5zf6_fit_tetramer1.pdb (#6) to map |
| 3425 | | cryosparc_P1_J138_004_volume_map_sharp.mrc (#2) using 8555 atoms |
| 3426 | | average map value = 0.129, steps = 2000 |
| 3427 | | shifted from previous position = 0.349 |
| 3428 | | rotated from previous position = 0.98 degrees |
| 3429 | | atoms outside contour = 4646, contour level = 0.10328 |
| 3430 | | |
| 3431 | | |
| 3432 | | > fitmap #6 inMap #2 moveWholeMolecules false |
| 3433 | | |
| 3434 | | Fit molecule hPNP_dimer_5zf6_fit_tetramer1.pdb (#6) to map |
| 3435 | | cryosparc_P1_J138_004_volume_map_sharp.mrc (#2) using 8555 atoms |
| 3436 | | average map value = 0.1277, steps = 2000 |
| 3437 | | shifted from previous position = 0.633 |
| 3438 | | rotated from previous position = 0.897 degrees |
| 3439 | | atoms outside contour = 4681, contour level = 0.10328 |
| 3440 | | |
| 3441 | | |
| 3442 | | > fitmap #6 inMap #2 moveWholeMolecules false |
| 3443 | | |
| 3444 | | Fit molecule hPNP_dimer_5zf6_fit_tetramer1.pdb (#6) to map |
| 3445 | | cryosparc_P1_J138_004_volume_map_sharp.mrc (#2) using 8555 atoms |
| 3446 | | average map value = 0.129, steps = 2000 |
| 3447 | | shifted from previous position = 0.41 |
| 3448 | | rotated from previous position = 0.127 degrees |
| 3449 | | atoms outside contour = 4685, contour level = 0.10328 |
| 3450 | | |
| 3451 | | |
| 3452 | | > fitmap #6 inMap #2 moveWholeMolecules false |
| 3453 | | |
| 3454 | | Fit molecule hPNP_dimer_5zf6_fit_tetramer1.pdb (#6) to map |
| 3455 | | cryosparc_P1_J138_004_volume_map_sharp.mrc (#2) using 8555 atoms |
| 3456 | | average map value = 0.1287, steps = 2000 |
| 3457 | | shifted from previous position = 0.256 |
| 3458 | | rotated from previous position = 0.952 degrees |
| 3459 | | atoms outside contour = 4632, contour level = 0.10328 |
| 3460 | | |
| 3461 | | |
| 3462 | | > fitmap #6 inMap #2 moveWholeMolecules false |
| 3463 | | |
| 3464 | | Fit molecule hPNP_dimer_5zf6_fit_tetramer1.pdb (#6) to map |
| 3465 | | cryosparc_P1_J138_004_volume_map_sharp.mrc (#2) using 8555 atoms |
| 3466 | | average map value = 0.1277, steps = 2000 |
| 3467 | | shifted from previous position = 0.662 |
| 3468 | | rotated from previous position = 1.12 degrees |
| 3469 | | atoms outside contour = 4683, contour level = 0.10328 |
| 3470 | | |
| 3471 | | |
| 3472 | | > fitmap #6 inMap #2 moveWholeMolecules false |
| 3473 | | |
| 3474 | | Fit molecule hPNP_dimer_5zf6_fit_tetramer1.pdb (#6) to map |
| 3475 | | cryosparc_P1_J138_004_volume_map_sharp.mrc (#2) using 8555 atoms |
| 3476 | | average map value = 0.1291, steps = 2000 |
| 3477 | | shifted from previous position = 0.449 |
| 3478 | | rotated from previous position = 0.373 degrees |
| 3479 | | atoms outside contour = 4684, contour level = 0.10328 |
| 3480 | | |
| 3481 | | |
| 3482 | | > fitmap #6 inMap #2 moveWholeMolecules false |
| 3483 | | |
| 3484 | | Fit molecule hPNP_dimer_5zf6_fit_tetramer1.pdb (#6) to map |
| 3485 | | cryosparc_P1_J138_004_volume_map_sharp.mrc (#2) using 8555 atoms |
| 3486 | | average map value = 0.129, steps = 2000 |
| 3487 | | shifted from previous position = 0.167 |
| 3488 | | rotated from previous position = 0.682 degrees |
| 3489 | | atoms outside contour = 4646, contour level = 0.10328 |
| 3490 | | |
| 3491 | | |
| 3492 | | > select #6/B:182 |
| 3493 | | |
| 3494 | | 8 atoms, 7 bonds, 1 residue, 1 model selected |
| 3495 | | |
| 3496 | | > select #6/B:182-304 |
| 3497 | | |
| 3498 | | 964 atoms, 983 bonds, 123 residues, 1 model selected |
| 3499 | | |
| 3500 | | > fitmap #6 inMap #2 moveWholeMolecules false |
| 3501 | | |
| 3502 | | Fit molecule hPNP_dimer_5zf6_fit_tetramer1.pdb (#6) to map |
| 3503 | | cryosparc_P1_J138_004_volume_map_sharp.mrc (#2) using 8555 atoms |
| 3504 | | average map value = 0.1275, steps = 2000 |
| 3505 | | shifted from previous position = 0.548 |
| 3506 | | rotated from previous position = 0.789 degrees |
| 3507 | | atoms outside contour = 4671, contour level = 0.10328 |
| 3508 | | |
| 3509 | | |
| 3510 | | > fitmap #6 inMap #2 moveWholeMolecules false |
| 3511 | | |
| 3512 | | Fit molecule hPNP_dimer_5zf6_fit_tetramer1.pdb (#6) to map |
| 3513 | | cryosparc_P1_J138_004_volume_map_sharp.mrc (#2) using 8555 atoms |
| 3514 | | average map value = 0.129, steps = 2000 |
| 3515 | | shifted from previous position = 0.33 |
| 3516 | | rotated from previous position = 0.366 degrees |
| 3517 | | atoms outside contour = 4685, contour level = 0.10328 |
| 3518 | | |
| 3519 | | |
| 3520 | | > fitmap #6 inMap #5 moveWholeMolecules false |
| 3521 | | |
| 3522 | | Fit molecule hPNP_dimer_5zf6_fit_tetramer1.pdb (#6) to map 5zf6.pdb map 4 (#5) |
| 3523 | | using 8555 atoms |
| 3524 | | average map value = 0.6589, steps = 44 |
| 3525 | | shifted from previous position = 0.3 |
| 3526 | | rotated from previous position = 0.943 degrees |
| 3527 | | atoms outside contour = 0, contour level = 0.10406 |
| 3528 | | |
| 3529 | | |
| 3530 | | > fitmap #6 inMap #2 moveWholeMolecules false |
| 3531 | | |
| 3532 | | Fit molecule hPNP_dimer_5zf6_fit_tetramer1.pdb (#6) to map |
| 3533 | | cryosparc_P1_J138_004_volume_map_sharp.mrc (#2) using 8555 atoms |
| 3534 | | average map value = 0.1275, steps = 2000 |
| 3535 | | shifted from previous position = 0.595 |
| 3536 | | rotated from previous position = 0.908 degrees |
| 3537 | | atoms outside contour = 4673, contour level = 0.10328 |
| 3538 | | |
| 3539 | | |
| 3540 | | > select #6/B:290 |
| 3541 | | |
| 3542 | | 8 atoms, 7 bonds, 1 residue, 1 model selected |
| 3543 | | |
| 3544 | | > select #6/B:290-291 |
| 3545 | | |
| 3546 | | 13 atoms, 12 bonds, 2 residues, 1 model selected |
| 3547 | | |
| 3548 | | > select #6/B:302 |
| 3549 | | |
| 3550 | | 12 atoms, 12 bonds, 1 residue, 1 model selected |
| 3551 | | |
| 3552 | | > select #6/B:302-373 |
| 3553 | | |
| 3554 | | 597 atoms, 605 bonds, 72 residues, 1 model selected |
| 3555 | | |
| 3556 | | > fitmap #6 inMap #2 moveWholeMolecules false |
| 3557 | | |
| 3558 | | Fit molecule hPNP_dimer_5zf6_fit_tetramer1.pdb (#6) to map |
| 3559 | | cryosparc_P1_J138_004_volume_map_sharp.mrc (#2) using 8555 atoms |
| 3560 | | average map value = 0.129, steps = 2000 |
| 3561 | | shifted from previous position = 0.319 |
| 3562 | | rotated from previous position = 0.348 degrees |
| 3563 | | atoms outside contour = 4688, contour level = 0.10328 |
| 3564 | | |
| 3565 | | |
| 3566 | | > fitmap #6 inMap #2 moveWholeMolecules false |
| 3567 | | |
| 3568 | | Fit molecule hPNP_dimer_5zf6_fit_tetramer1.pdb (#6) to map |
| 3569 | | cryosparc_P1_J138_004_volume_map_sharp.mrc (#2) using 8555 atoms |
| 3570 | | average map value = 0.129, steps = 2000 |
| 3571 | | shifted from previous position = 0.331 |
| 3572 | | rotated from previous position = 0.953 degrees |
| 3573 | | atoms outside contour = 4637, contour level = 0.10328 |
| 3574 | | |
| 3575 | | |
| 3576 | | > fitmap #6 inMap #2 moveWholeMolecules false |
| 3577 | | |
| 3578 | | Fit molecule hPNP_dimer_5zf6_fit_tetramer1.pdb (#6) to map |
| 3579 | | cryosparc_P1_J138_004_volume_map_sharp.mrc (#2) using 8555 atoms |
| 3580 | | average map value = 0.1278, steps = 2000 |
| 3581 | | shifted from previous position = 0.601 |
| 3582 | | rotated from previous position = 0.815 degrees |
| 3583 | | atoms outside contour = 4675, contour level = 0.10328 |
| 3584 | | |
| 3585 | | |
| 3586 | | > fitmap #6 inMap #2 moveWholeMolecules false |
| 3587 | | |
| 3588 | | Fit molecule hPNP_dimer_5zf6_fit_tetramer1.pdb (#6) to map |
| 3589 | | cryosparc_P1_J138_004_volume_map_sharp.mrc (#2) using 8555 atoms |
| 3590 | | average map value = 0.129, steps = 2000 |
| 3591 | | shifted from previous position = 0.403 |
| 3592 | | rotated from previous position = 0.116 degrees |
| 3593 | | atoms outside contour = 4686, contour level = 0.10328 |
| 3594 | | |
| 3595 | | |
| 3596 | | > fitmap #6 inMap #2 moveWholeMolecules false |
| 3597 | | |
| 3598 | | Fit molecule hPNP_dimer_5zf6_fit_tetramer1.pdb (#6) to map |
| 3599 | | cryosparc_P1_J138_004_volume_map_sharp.mrc (#2) using 8555 atoms |
| 3600 | | average map value = 0.1287, steps = 2000 |
| 3601 | | shifted from previous position = 0.254 |
| 3602 | | rotated from previous position = 0.971 degrees |
| 3603 | | atoms outside contour = 4636, contour level = 0.10328 |
| 3604 | | |
| 3605 | | |
| 3606 | | > select clear |
| 3607 | | |
| 3608 | | > show #!4 models |
| 3609 | | |
| 3610 | | > select add #4 |
| 3611 | | |
| 3612 | | 8555 atoms, 8618 bonds, 1174 residues, 1 model selected |
| 3613 | | |
| 3614 | | > view matrix models |
| 3615 | | > #4,0.41896,-0.90784,0.01751,172.56,0.15936,0.092502,0.98288,84.02,-0.89391,-0.40899,0.18343,344.93 |
| 3616 | | |
| 3617 | | > ui mousemode right "translate selected models" |
| 3618 | | |
| 3619 | | > view matrix models |
| 3620 | | > #4,0.41896,-0.90784,0.01751,178.41,0.15936,0.092502,0.98288,144.07,-0.89391,-0.40899,0.18343,218.26 |
| 3621 | | |
| 3622 | | > ui mousemode right "rotate selected models" |
| 3623 | | |
| 3624 | | > view matrix models |
| 3625 | | > #4,-0.42775,0.90035,0.079962,224.76,0.60709,0.22062,0.76339,113.04,0.66968,0.37509,-0.64097,107.87 |
| 3626 | | |
| 3627 | | > view matrix models |
| 3628 | | > #4,-0.18295,0.98312,0.003384,206.1,0.40212,0.071688,0.91278,126.6,0.89712,0.16835,-0.40844,83.19 |
| 3629 | | |
| 3630 | | > hide #!6 models |
| 3631 | | |
| 3632 | | > show #!6 models |
| 3633 | | |
| 3634 | | > fitmap #6 inMap #2 |
| 3635 | | |
| 3636 | | Fit molecule hPNP_dimer_5zf6_fit_tetramer1.pdb (#6) to map |
| 3637 | | cryosparc_P1_J138_004_volume_map_sharp.mrc (#2) using 8555 atoms |
| 3638 | | average map value = 0.1277, steps = 2000 |
| 3639 | | shifted from previous position = 0.66 |
| 3640 | | rotated from previous position = 1.14 degrees |
| 3641 | | atoms outside contour = 4682, contour level = 0.10328 |
| 3642 | | |
| 3643 | | Position of hPNP_dimer_5zf6_fit_tetramer1.pdb (#6) relative to |
| 3644 | | cryosparc_P1_J138_004_volume_map_sharp.mrc (#2) coordinates: |
| 3645 | | Matrix rotation and translation |
| 3646 | | 0.98050817 -0.05115886 -0.18970108 13.07808380 |
| 3647 | | -0.00988416 0.95144047 -0.30767407 32.79012008 |
| 3648 | | 0.19622954 0.30355198 0.93238949 -110.39635553 |
| 3649 | | Axis 0.84418066 -0.53301911 0.05700559 |
| 3650 | | Axis point 0.00000000 367.20770125 26.56375354 |
| 3651 | | Rotation angle (degrees) 21.22450246 |
| 3652 | | Shift along axis -12.73070479 |
| 3653 | | |
| 3654 | | |
| 3655 | | > view matrix models |
| 3656 | | > #4,-0.08736,0.99518,0.044597,196.53,0.42306,-0.0034671,0.90609,126.01,0.90188,0.098024,-0.42072,84.082 |
| 3657 | | |
| 3658 | | > ui mousemode right "translate selected models" |
| 3659 | | |
| 3660 | | > view matrix models |
| 3661 | | > #4,-0.08736,0.99518,0.044597,191.27,0.42306,-0.0034671,0.90609,129.79,0.90188,0.098024,-0.42072,71.859 |
| 3662 | | |
| 3663 | | > view matrix models |
| 3664 | | > #4,-0.08736,0.99518,0.044597,183.77,0.42306,-0.0034671,0.90609,130.43,0.90188,0.098024,-0.42072,77.917 |
| 3665 | | |
| 3666 | | > fitmap #1 inMap #2 |
| 3667 | | |
| 3668 | | Fit molecule 5zf6.pdb (#1) to map cryosparc_P1_J138_004_volume_map_sharp.mrc |
| 3669 | | (#2) using 8555 atoms |
| 3670 | | average map value = 0.1277, steps = 2000 |
| 3671 | | shifted from previous position = 0.621 |
| 3672 | | rotated from previous position = 1.01 degrees |
| 3673 | | atoms outside contour = 4676, contour level = 0.10328 |
| 3674 | | |
| 3675 | | Position of 5zf6.pdb (#1) relative to |
| 3676 | | cryosparc_P1_J138_004_volume_map_sharp.mrc (#2) coordinates: |
| 3677 | | Matrix rotation and translation |
| 3678 | | 0.97947769 0.14877998 -0.13597045 146.59186252 |
| 3679 | | 0.10549263 0.19640199 0.97483206 145.59322335 |
| 3680 | | 0.17174037 -0.96917014 0.17667618 184.27065038 |
| 3681 | | Axis -0.98746437 -0.15630305 -0.02198800 |
| 3682 | | Axis point 0.00000000 170.41082713 18.63330543 |
| 3683 | | Rotation angle (degrees) 79.84696577 |
| 3684 | | Shift along axis -171.56264926 |
| 3685 | | |
| 3686 | | |
| 3687 | | > fitmap #4 inMap #2 |
| 3688 | | |
| 3689 | | Fit molecule 5zf6.pdb (#4) to map cryosparc_P1_J138_004_volume_map_sharp.mrc |
| 3690 | | (#2) using 8555 atoms |
| 3691 | | average map value = 0.111, steps = 1076 |
| 3692 | | shifted from previous position = 4.65 |
| 3693 | | rotated from previous position = 13.2 degrees |
| 3694 | | atoms outside contour = 5073, contour level = 0.10328 |
| 3695 | | |
| 3696 | | Position of 5zf6.pdb (#4) relative to |
| 3697 | | cryosparc_P1_J138_004_volume_map_sharp.mrc (#2) coordinates: |
| 3698 | | Matrix rotation and translation |
| 3699 | | -0.36553432 0.89395507 0.25928552 159.59107335 |
| 3700 | | 0.35313265 -0.12454696 0.92724612 89.23861751 |
| 3701 | | 0.86120960 0.43050246 -0.27015854 37.52485026 |
| 3702 | | Axis -0.52316181 -0.63393602 -0.56958480 |
| 3703 | | Axis point 53.81283798 -0.00000000 -6.36388496 |
| 3704 | | Rotation angle (degrees) 151.65683196 |
| 3705 | | Shift along axis -161.43711324 |
| 3706 | | |
| 3707 | | |
| 3708 | | > fitmap #5 inMap #2 |
| 3709 | | |
| 3710 | | Fit map 5zf6.pdb map 4 in map cryosparc_P1_J138_004_volume_map_sharp.mrc using |
| 3711 | | 13249 points |
| 3712 | | correlation = 0.5788, correlation about mean = 0.198, overlap = 733.5 |
| 3713 | | steps = 2000, shift = 0.484, angle = 0.682 degrees |
| 3714 | | |
| 3715 | | Position of 5zf6.pdb map 4 (#5) relative to |
| 3716 | | cryosparc_P1_J138_004_volume_map_sharp.mrc (#2) coordinates: |
| 3717 | | Matrix rotation and translation |
| 3718 | | 0.95807999 0.12330246 -0.25861017 146.85960897 |
| 3719 | | 0.23030716 0.20545224 0.95118241 145.69179160 |
| 3720 | | 0.17041517 -0.97086861 0.16844224 183.83696417 |
| 3721 | | Axis -0.97454450 -0.21753027 0.05425498 |
| 3722 | | Axis point 0.00000000 173.40314082 29.37698708 |
| 3723 | | Rotation angle (degrees) 80.44541010 |
| 3724 | | Shift along axis -164.83952771 |
| 3725 | | |
| 3726 | | |
| 3727 | | > select add #1 |
| 3728 | | |
| 3729 | | 17110 atoms, 17236 bonds, 2348 residues, 2 models selected |
| 3730 | | |
| 3731 | | > show #!1 models |
| 3732 | | |
| 3733 | | > hide #!6 models |
| 3734 | | |
| 3735 | | > select subtract #4 |
| 3736 | | |
| 3737 | | 8555 atoms, 8618 bonds, 1174 residues, 1 model selected |
| 3738 | | |
| 3739 | | > fitmap #5 inMap #2 |
| 3740 | | |
| 3741 | | Fit map 5zf6.pdb map 4 in map cryosparc_P1_J138_004_volume_map_sharp.mrc using |
| 3742 | | 13249 points |
| 3743 | | correlation = 0.5837, correlation about mean = 0.2048, overlap = 738.4 |
| 3744 | | steps = 2000, shift = 0.311, angle = 0.305 degrees |
| 3745 | | |
| 3746 | | Position of 5zf6.pdb map 4 (#5) relative to |
| 3747 | | cryosparc_P1_J138_004_volume_map_sharp.mrc (#2) coordinates: |
| 3748 | | Matrix rotation and translation |
| 3749 | | 0.95689175 0.12490398 -0.26221586 146.68598283 |
| 3750 | | 0.23295357 0.20914731 0.94973155 145.87551846 |
| 3751 | | 0.17346699 -0.96987442 0.17103451 183.65499502 |
| 3752 | | Axis -0.97373187 -0.22100279 0.05480881 |
| 3753 | | Axis point 0.00000000 173.59451621 29.35452178 |
| 3754 | | Rotation angle (degrees) 80.29724460 |
| 3755 | | Shift along axis -165.00580142 |
| 3756 | | |
| 3757 | | |
| 3758 | | > fitmap #5 inMap #2 |
| 3759 | | |
| 3760 | | Fit map 5zf6.pdb map 4 in map cryosparc_P1_J138_004_volume_map_sharp.mrc using |
| 3761 | | 13249 points |
| 3762 | | correlation = 0.5802, correlation about mean = 0.2023, overlap = 737.3 |
| 3763 | | steps = 2000, shift = 0.297, angle = 0.896 degrees |
| 3764 | | |
| 3765 | | Position of 5zf6.pdb map 4 (#5) relative to |
| 3766 | | cryosparc_P1_J138_004_volume_map_sharp.mrc (#2) coordinates: |
| 3767 | | Matrix rotation and translation |
| 3768 | | 0.95523339 0.12648677 -0.26745139 146.55407822 |
| 3769 | | 0.24029680 0.19565106 0.95077763 145.98961234 |
| 3770 | | 0.17258794 -0.97248226 0.15649806 183.41632773 |
| 3771 | | Axis -0.97319253 -0.22266517 0.05758923 |
| 3772 | | Axis point 0.00000000 171.93881899 30.45197129 |
| 3773 | | Rotation angle (degrees) 81.15909876 |
| 3774 | | Shift along axis -164.56933019 |
| 3775 | | |
| 3776 | | |
| 3777 | | > fitmap #1 inMap #2 |
| 3778 | | |
| 3779 | | Fit molecule 5zf6.pdb (#1) to map cryosparc_P1_J138_004_volume_map_sharp.mrc |
| 3780 | | (#2) using 8555 atoms |
| 3781 | | average map value = 0.1291, steps = 2000 |
| 3782 | | shifted from previous position = 0.392 |
| 3783 | | rotated from previous position = 0.273 degrees |
| 3784 | | atoms outside contour = 4687, contour level = 0.10328 |
| 3785 | | |
| 3786 | | Position of 5zf6.pdb (#1) relative to |
| 3787 | | cryosparc_P1_J138_004_volume_map_sharp.mrc (#2) coordinates: |
| 3788 | | Matrix rotation and translation |
| 3789 | | 0.97915094 0.14700446 -0.14018954 146.62405858 |
| 3790 | | 0.11011085 0.19584575 0.97443318 145.92300788 |
| 3791 | | 0.17070156 -0.96955356 0.17557577 184.05988413 |
| 3792 | | Axis -0.98727891 -0.15789008 -0.01873690 |
| 3793 | | Axis point 0.00000000 170.57529358 18.76382801 |
| 3794 | | Rotation angle (degrees) 79.90468443 |
| 3795 | | Shift along axis -171.24734835 |
| 3796 | | |
| 3797 | | |
| 3798 | | > fitmap #3 inMap #2 |
| 3799 | | |
| 3800 | | Fit molecule hPNP_dimer_5zf6_fit_tetramer2.pdb (#3) to map |
| 3801 | | cryosparc_P1_J138_004_volume_map_sharp.mrc (#2) using 8555 atoms |
| 3802 | | average map value = 0.02577, steps = 116 |
| 3803 | | shifted from previous position = 8.58 |
| 3804 | | rotated from previous position = 7.99 degrees |
| 3805 | | atoms outside contour = 7667, contour level = 0.10328 |
| 3806 | | |
| 3807 | | Position of hPNP_dimer_5zf6_fit_tetramer2.pdb (#3) relative to |
| 3808 | | cryosparc_P1_J138_004_volume_map_sharp.mrc (#2) coordinates: |
| 3809 | | Matrix rotation and translation |
| 3810 | | 0.68645478 0.37419295 0.62350577 -153.15730085 |
| 3811 | | 0.19540344 0.73097981 -0.65382413 67.23553836 |
| 3812 | | -0.70042652 0.57065588 0.42866600 94.59887008 |
| 3813 | | Axis 0.67568241 0.73056133 -0.09865815 |
| 3814 | | Axis point -12.74486076 0.00000000 170.20042114 |
| 3815 | | Rotation angle (degrees) 64.97268395 |
| 3816 | | Shift along axis -63.69895833 |
| 3817 | | |
| 3818 | | |
| 3819 | | > fitmap #4 inMap #2 |
| 3820 | | |
| 3821 | | Fit molecule 5zf6.pdb (#4) to map cryosparc_P1_J138_004_volume_map_sharp.mrc |
| 3822 | | (#2) using 8555 atoms |
| 3823 | | average map value = 0.111, steps = 628 |
| 3824 | | shifted from previous position = 0.0142 |
| 3825 | | rotated from previous position = 0.0153 degrees |
| 3826 | | atoms outside contour = 5070, contour level = 0.10328 |
| 3827 | | |
| 3828 | | Position of 5zf6.pdb (#4) relative to |
| 3829 | | cryosparc_P1_J138_004_volume_map_sharp.mrc (#2) coordinates: |
| 3830 | | Matrix rotation and translation |
| 3831 | | -0.36562224 0.89396097 0.25914118 159.60672611 |
| 3832 | | 0.35289438 -0.12448775 0.92734478 89.24870916 |
| 3833 | | 0.86126995 0.43050734 -0.26995831 37.49952053 |
| 3834 | | Axis -0.52308545 -0.63393934 -0.56965124 |
| 3835 | | Axis point 53.83227055 0.00000000 -6.37339730 |
| 3836 | | Rotation angle (degrees) 151.64648359 |
| 3837 | | Shift along axis -161.42787146 |
| 3838 | | |
| 3839 | | |
| 3840 | | > fitmap #5 inMap #2 |
| 3841 | | |
| 3842 | | Fit map 5zf6.pdb map 4 in map cryosparc_P1_J138_004_volume_map_sharp.mrc using |
| 3843 | | 13249 points |
| 3844 | | correlation = 0.5788, correlation about mean = 0.198, overlap = 733.5 |
| 3845 | | steps = 2000, shift = 0.601, angle = 0.852 degrees |
| 3846 | | |
| 3847 | | Position of 5zf6.pdb map 4 (#5) relative to |
| 3848 | | cryosparc_P1_J138_004_volume_map_sharp.mrc (#2) coordinates: |
| 3849 | | Matrix rotation and translation |
| 3850 | | 0.95808182 0.12330121 -0.25860399 146.85969531 |
| 3851 | | 0.23030239 0.20544566 0.95118499 145.69187618 |
| 3852 | | 0.17041133 -0.97087016 0.16843719 183.83677193 |
| 3853 | | Axis -0.97454577 -0.21752501 0.05425315 |
| 3854 | | Axis point 0.00000000 173.40254461 29.37657817 |
| 3855 | | Rotation angle (degrees) 80.44569473 |
| 3856 | | Shift along axis -164.83939909 |
| 3857 | | |
| 3858 | | |
| 3859 | | > fitmap #5 inMap #2 |
| 3860 | | |
| 3861 | | Fit map 5zf6.pdb map 4 in map cryosparc_P1_J138_004_volume_map_sharp.mrc using |
| 3862 | | 13249 points |
| 3863 | | correlation = 0.5837, correlation about mean = 0.2048, overlap = 738.4 |
| 3864 | | steps = 2000, shift = 0.311, angle = 0.306 degrees |
| 3865 | | |
| 3866 | | Position of 5zf6.pdb map 4 (#5) relative to |
| 3867 | | cryosparc_P1_J138_004_volume_map_sharp.mrc (#2) coordinates: |
| 3868 | | Matrix rotation and translation |
| 3869 | | 0.95689295 0.12490402 -0.26221149 146.68601347 |
| 3870 | | 0.23294922 0.20914729 0.94973262 145.87546726 |
| 3871 | | 0.17346624 -0.96987442 0.17103528 183.65508357 |
| 3872 | | Axis -0.97373258 -0.22100023 0.05480660 |
| 3873 | | Axis point 0.00000000 173.59456162 29.35417238 |
| 3874 | | Rotation angle (degrees) 80.29718783 |
| 3875 | | Shift along axis -165.00595139 |
| 3876 | | |
| 3877 | | |
| 3878 | | > select add #6 |
| 3879 | | |
| 3880 | | 17110 atoms, 17236 bonds, 2348 residues, 2 models selected |
| 3881 | | |
| 3882 | | > show #!5 models |
| 3883 | | |
| 3884 | | > hide #!5 models |
| 3885 | | |
| 3886 | | > select add #4 |
| 3887 | | |
| 3888 | | 25665 atoms, 25854 bonds, 3522 residues, 3 models selected |
| 3889 | | |
| 3890 | | > select subtract #4 |
| 3891 | | |
| 3892 | | 17110 atoms, 17236 bonds, 2348 residues, 2 models selected |
| 3893 | | |
| 3894 | | > select subtract #6 |
| 3895 | | |
| 3896 | | 8555 atoms, 8618 bonds, 1174 residues, 1 model selected |
| 3897 | | |
| 3898 | | > select add #6 |
| 3899 | | |
| 3900 | | 17110 atoms, 17236 bonds, 2348 residues, 2 models selected |
| 3901 | | |
| 3902 | | > select subtract #1 |
| 3903 | | |
| 3904 | | 8555 atoms, 8618 bonds, 1174 residues, 1 model selected |
| 3905 | | |
| 3906 | | > fitmap #6 inMap #5 |
| 3907 | | |
| 3908 | | Fit molecule hPNP_dimer_5zf6_fit_tetramer1.pdb (#6) to map 5zf6.pdb map 4 (#5) |
| 3909 | | using 8555 atoms |
| 3910 | | average map value = 0.6588, steps = 48 |
| 3911 | | shifted from previous position = 0.493 |
| 3912 | | rotated from previous position = 0.363 degrees |
| 3913 | | atoms outside contour = 0, contour level = 0.10406 |
| 3914 | | |
| 3915 | | Position of hPNP_dimer_5zf6_fit_tetramer1.pdb (#6) relative to 5zf6.pdb map 4 |
| 3916 | | (#5) coordinates: |
| 3917 | | Matrix rotation and translation |
| 3918 | | 0.97055165 0.22364863 -0.08950306 -205.36240728 |
| 3919 | | -0.06949210 -0.09581212 -0.99297075 244.22612919 |
| 3920 | | -0.23065202 0.96994914 -0.07744880 -124.28462420 |
| 3921 | | Axis 0.98654027 0.07093980 -0.14732906 |
| 3922 | | Axis point 0.00000000 197.65683581 39.75508724 |
| 3923 | | Rotation angle (degrees) 95.81718147 |
| 3924 | | Shift along axis -166.96219566 |
| 3925 | | |
| 3926 | | |
| 3927 | | > fitmap #6 inMap #5 |
| 3928 | | |
| 3929 | | Fit molecule hPNP_dimer_5zf6_fit_tetramer1.pdb (#6) to map 5zf6.pdb map 4 (#5) |
| 3930 | | using 8555 atoms |
| 3931 | | average map value = 0.6589, steps = 40 |
| 3932 | | shifted from previous position = 0.019 |
| 3933 | | rotated from previous position = 0.00359 degrees |
| 3934 | | atoms outside contour = 0, contour level = 0.10406 |
| 3935 | | |
| 3936 | | Position of hPNP_dimer_5zf6_fit_tetramer1.pdb (#6) relative to 5zf6.pdb map 4 |
| 3937 | | (#5) coordinates: |
| 3938 | | Matrix rotation and translation |
| 3939 | | 0.97055020 0.22365652 -0.08949909 -205.37186328 |
| 3940 | | -0.06950193 -0.09575101 -0.99297595 244.20964705 |
| 3941 | | -0.23065517 0.96995336 -0.07738660 -124.28250667 |
| 3942 | | Axis 0.98653885 0.07094293 -0.14733704 |
| 3943 | | Axis point 0.00000000 197.65379741 39.75494966 |
| 3944 | | Rotation angle (degrees) 95.81367267 |
| 3945 | | Shift along axis -166.97095635 |
| 3946 | | |
| 3947 | | |
| 3948 | | > fitmap #6 inMap #5 |
| 3949 | | |
| 3950 | | Fit molecule hPNP_dimer_5zf6_fit_tetramer1.pdb (#6) to map 5zf6.pdb map 4 (#5) |
| 3951 | | using 8555 atoms |
| 3952 | | average map value = 0.6589, steps = 40 |
| 3953 | | shifted from previous position = 0.0306 |
| 3954 | | rotated from previous position = 0.00975 degrees |
| 3955 | | atoms outside contour = 0, contour level = 0.10406 |
| 3956 | | |
| 3957 | | Position of hPNP_dimer_5zf6_fit_tetramer1.pdb (#6) relative to 5zf6.pdb map 4 |
| 3958 | | (#5) coordinates: |
| 3959 | | Matrix rotation and translation |
| 3960 | | 0.97055232 0.22364004 -0.08951726 -205.34348872 |
| 3961 | | -0.06955967 -0.09559197 -0.99298723 244.19422434 |
| 3962 | | -0.23062884 0.96997285 -0.07722068 -124.30528679 |
| 3963 | | Axis 0.98653781 0.07091938 -0.14735532 |
| 3964 | | Axis point 0.00000000 197.66396599 39.75351657 |
| 3965 | | Rotation angle (degrees) 95.80425393 |
| 3966 | | Shift along axis -166.94396819 |
| 3967 | | |
| 3968 | | |
| 3969 | | > fitmap #1 inMap #5 |
| 3970 | | |
| 3971 | | Fit molecule 5zf6.pdb (#1) to map 5zf6.pdb map 4 (#5) using 8555 atoms |
| 3972 | | average map value = 0.6588, steps = 48 |
| 3973 | | shifted from previous position = 0.161 |
| 3974 | | rotated from previous position = 0.509 degrees |
| 3975 | | atoms outside contour = 0, contour level = 0.10406 |
| 3976 | | |
| 3977 | | Position of 5zf6.pdb (#1) relative to 5zf6.pdb map 4 (#5) coordinates: |
| 3978 | | Matrix rotation and translation |
| 3979 | | 0.99145298 0.01667906 0.12939396 0.08470249 |
| 3980 | | -0.01973646 0.99955465 0.02238229 -0.25108805 |
| 3981 | | -0.12896302 -0.02474477 0.99134063 0.07775739 |
| 3982 | | Axis -0.17774891 0.97444383 -0.13734824 |
| 3983 | | Axis point 0.33249228 0.00000000 -0.26118801 |
| 3984 | | Rotation angle (degrees) 7.61791888 |
| 3985 | | Shift along axis -0.27040682 |
| 3986 | | |
| 3987 | | |
| 3988 | | > fitmap #1 inMap #5 |
| 3989 | | |
| 3990 | | Fit molecule 5zf6.pdb (#1) to map 5zf6.pdb map 4 (#5) using 8555 atoms |
| 3991 | | average map value = 0.6589, steps = 40 |
| 3992 | | shifted from previous position = 0.0407 |
| 3993 | | rotated from previous position = 0.0102 degrees |
| 3994 | | atoms outside contour = 0, contour level = 0.10406 |
| 3995 | | |
| 3996 | | Position of 5zf6.pdb (#1) relative to 5zf6.pdb map 4 (#5) coordinates: |
| 3997 | | Matrix rotation and translation |
| 3998 | | 0.99146916 0.01666799 0.12927133 0.06971343 |
| 3999 | | -0.01970608 0.99955802 0.02225820 -0.21745944 |
| 4000 | | -0.12884319 -0.02461575 0.99135942 0.06043299 |
| 4001 | | Axis -0.17698575 0.97458383 -0.13734048 |
| 4002 | | Axis point 0.23490649 0.00000000 -0.20536492 |
| 4003 | | Rotation angle (degrees) 7.60962745 |
| 4004 | | Shift along axis -0.23257063 |
| 4005 | | |
| 4006 | | |
| 4007 | | > fitmap #3 inMap #5 |
| 4008 | | |
| 4009 | | Fit molecule hPNP_dimer_5zf6_fit_tetramer2.pdb (#3) to map 5zf6.pdb map 4 (#5) |
| 4010 | | using 8555 atoms |
| 4011 | | average map value = 0.1347, steps = 208 |
| 4012 | | shifted from previous position = 25.2 |
| 4013 | | rotated from previous position = 35.7 degrees |
| 4014 | | atoms outside contour = 6498, contour level = 0.10406 |
| 4015 | | |
| 4016 | | Position of hPNP_dimer_5zf6_fit_tetramer2.pdb (#3) relative to 5zf6.pdb map 4 |
| 4017 | | (#5) coordinates: |
| 4018 | | Matrix rotation and translation |
| 4019 | | 0.77158106 0.62513280 0.11777791 -289.07448145 |
| 4020 | | 0.63513719 -0.74671117 -0.19754285 78.40770373 |
| 4021 | | -0.03554444 0.22722545 -0.97319329 91.27335582 |
| 4022 | | Axis 0.94037000 0.33943148 0.02214813 |
| 4023 | | Axis point 0.00000000 85.49559070 58.83183688 |
| 4024 | | Rotation angle (degrees) 166.94703469 |
| 4025 | | Shift along axis -243.20139227 |
| 4026 | | |
| 4027 | | |
| 4028 | | > fitmap #4 inMap #5 |
| 4029 | | |
| 4030 | | Fit molecule 5zf6.pdb (#4) to map 5zf6.pdb map 4 (#5) using 8555 atoms |
| 4031 | | average map value = 0.1942, steps = 288 |
| 4032 | | shifted from previous position = 83.6 |
| 4033 | | rotated from previous position = 22.3 degrees |
| 4034 | | atoms outside contour = 4391, contour level = 0.10406 |
| 4035 | | |
| 4036 | | Position of 5zf6.pdb (#4) relative to 5zf6.pdb map 4 (#5) coordinates: |
| 4037 | | Matrix rotation and translation |
| 4038 | | 0.19636392 0.91428712 0.35428840 -37.79820017 |
| 4039 | | -0.65264442 -0.14778711 0.74311118 24.99961702 |
| 4040 | | 0.73177624 -0.37714458 0.56768433 -77.46490126 |
| 4041 | | Axis -0.57073182 -0.19231708 -0.79829777 |
| 4042 | | Axis point 29.48192807 18.18756670 0.00000000 |
| 4043 | | Rotation angle (degrees) 101.06190229 |
| 4044 | | Shift along axis 78.60484018 |
| 4045 | | |
| 4046 | | |
| 4047 | | > show #!6 models |
| 4048 | | |
| 4049 | | > hide #!4 models |
| 4050 | | |
| 4051 | | > show #!4 models |
| 4052 | | |
| 4053 | | > hide #!1 models |
| 4054 | | |
| 4055 | | > fitmap #6 inMap #5 |
| 4056 | | |
| 4057 | | Fit molecule hPNP_dimer_5zf6_fit_tetramer1.pdb (#6) to map 5zf6.pdb map 4 (#5) |
| 4058 | | using 8555 atoms |
| 4059 | | average map value = 0.6589, steps = 40 |
| 4060 | | shifted from previous position = 0.025 |
| 4061 | | rotated from previous position = 0.006 degrees |
| 4062 | | atoms outside contour = 0, contour level = 0.10406 |
| 4063 | | |
| 4064 | | Position of hPNP_dimer_5zf6_fit_tetramer1.pdb (#6) relative to 5zf6.pdb map 4 |
| 4065 | | (#5) coordinates: |
| 4066 | | Matrix rotation and translation |
| 4067 | | 0.97055420 0.22364475 -0.08948515 -205.36895267 |
| 4068 | | -0.06950521 -0.09568124 -0.99298245 244.19933684 |
| 4069 | | -0.23063735 0.96996296 -0.07731936 -124.29858846 |
| 4070 | | Axis 0.98653982 0.07094047 -0.14733171 |
| 4071 | | Axis point 0.00000000 197.65981342 39.75092422 |
| 4072 | | Rotation angle (degrees) 95.80961211 |
| 4073 | | Shift along axis -166.96791002 |
| 4074 | | |
| 4075 | | |
| 4076 | | > fitmap #6 inMap #2 |
| 4077 | | |
| 4078 | | Fit molecule hPNP_dimer_5zf6_fit_tetramer1.pdb (#6) to map |
| 4079 | | cryosparc_P1_J138_004_volume_map_sharp.mrc (#2) using 8555 atoms |
| 4080 | | average map value = 0.1297, steps = 224 |
| 4081 | | shifted from previous position = 0.231 |
| 4082 | | rotated from previous position = 0.288 degrees |
| 4083 | | atoms outside contour = 4645, contour level = 0.10328 |
| 4084 | | |
| 4085 | | Position of hPNP_dimer_5zf6_fit_tetramer1.pdb (#6) relative to |
| 4086 | | cryosparc_P1_J138_004_volume_map_sharp.mrc (#2) coordinates: |
| 4087 | | Matrix rotation and translation |
| 4088 | | 0.98003837 -0.05662787 -0.19057303 14.34462696 |
| 4089 | | -0.00335724 0.95372551 -0.30065992 30.04155398 |
| 4090 | | 0.19878009 0.29529806 0.93449748 -109.97811233 |
| 4091 | | Axis 0.83483592 -0.54541761 0.07462310 |
| 4092 | | Axis point 0.00000000 373.57563125 19.53424803 |
| 4093 | | Rotation angle (degrees) 20.91184972 |
| 4094 | | Shift along axis -12.61669073 |
| 4095 | | |
| 4096 | | |
| 4097 | | > select subtract #6 |
| 4098 | | |
| 4099 | | Nothing selected |
| 4100 | | |
| 4101 | | > hide #!6 models |
| 4102 | | |
| 4103 | | > show #!1 models |
| 4104 | | |
| 4105 | | > fitmap #1 inMap #5 |
| 4106 | | |
| 4107 | | Fit molecule 5zf6.pdb (#1) to map 5zf6.pdb map 4 (#5) using 8555 atoms |
| 4108 | | average map value = 0.6589, steps = 44 |
| 4109 | | shifted from previous position = 0.026 |
| 4110 | | rotated from previous position = 0.0331 degrees |
| 4111 | | atoms outside contour = 0, contour level = 0.10406 |
| 4112 | | |
| 4113 | | Position of 5zf6.pdb (#1) relative to 5zf6.pdb map 4 (#5) coordinates: |
| 4114 | | Matrix rotation and translation |
| 4115 | | 0.99146060 0.01666840 0.12933693 0.08477383 |
| 4116 | | -0.01963405 0.99957196 0.02168854 -0.22398077 |
| 4117 | | -0.12892005 -0.02404274 0.99136349 0.08044059 |
| 4118 | | Axis -0.17271744 0.97538220 -0.13710670 |
| 4119 | | Axis point 0.38468345 0.00000000 -0.30402781 |
| 4120 | | Rotation angle (degrees) 7.60758331 |
| 4121 | | Shift along axis -0.24413772 |
| 4122 | | |
| 4123 | | |
| 4124 | | > fitmap #1 inMap #5 moveWholeMolecules false |
| 4125 | | |
| 4126 | | Fit molecule 5zf6.pdb (#1) to map 5zf6.pdb map 4 (#5) using 8555 atoms |
| 4127 | | average map value = 0.6589, steps = 40 |
| 4128 | | shifted from previous position = 0.0313 |
| 4129 | | rotated from previous position = 0.0104 degrees |
| 4130 | | atoms outside contour = 0, contour level = 0.10406 |
| 4131 | | |
| 4132 | | |
| 4133 | | > ui tool show "Fit in Map" |
| 4134 | | |
| 4135 | | > close #5 |
| 4136 | | |
| 4137 | | > select :200-320 |
| 4138 | | |
| 4139 | | 7696 atoms, 7816 bonds, 968 residues, 4 models selected |
| 4140 | | |
| 4141 | | > select :250-350 |
| 4142 | | |
| 4143 | | 6712 atoms, 6840 bonds, 808 residues, 4 models selected |
| 4144 | | |
| 4145 | | > select :270-350 |
| 4146 | | |
| 4147 | | 5440 atoms, 5560 bonds, 648 residues, 4 models selected |
| 4148 | | |
| 4149 | | > fitmap #1 inMap #2 moveWholeMolecules false |
| 4150 | | |
| 4151 | | Fit molecule 5zf6.pdb (#1) to map cryosparc_P1_J138_004_volume_map_sharp.mrc |
| 4152 | | (#2) using 8555 atoms |
| 4153 | | average map value = 0.1289, steps = 2000 |
| 4154 | | shifted from previous position = 0.175 |
| 4155 | | rotated from previous position = 0.694 degrees |
| 4156 | | atoms outside contour = 4632, contour level = 0.10328 |
| 4157 | | |
| 4158 | | |
| 4159 | | > fitmap #1 inMap #2 moveWholeMolecules false rotate false |
| 4160 | | |
| 4161 | | Fit molecule 5zf6.pdb (#1) to map cryosparc_P1_J138_004_volume_map_sharp.mrc |
| 4162 | | (#2) using 8555 atoms |
| 4163 | | average map value = 0.1293, steps = 48 |
| 4164 | | shifted from previous position = 0.142 |
| 4165 | | rotated from previous position = 0 degrees |
| 4166 | | atoms outside contour = 4629, contour level = 0.10328 |
| 4167 | | |
| 4168 | | |
| 4169 | | > fitmap #1 inMap #2 moveWholeMolecules false rotate false |
| 4170 | | |
| 4171 | | Fit molecule 5zf6.pdb (#1) to map cryosparc_P1_J138_004_volume_map_sharp.mrc |
| 4172 | | (#2) using 8555 atoms |
| 4173 | | average map value = 0.1293, steps = 40 |
| 4174 | | shifted from previous position = 0.0151 |
| 4175 | | rotated from previous position = 0 degrees |
| 4176 | | atoms outside contour = 4630, contour level = 0.10328 |
| 4177 | | |
| 4178 | | |
| 4179 | | > fitmap #1 inMap #2 moveWholeMolecules false rotate false |
| 4180 | | |
| 4181 | | Fit molecule 5zf6.pdb (#1) to map cryosparc_P1_J138_004_volume_map_sharp.mrc |
| 4182 | | (#2) using 8555 atoms |
| 4183 | | average map value = 0.1293, steps = 40 |
| 4184 | | shifted from previous position = 0.0142 |
| 4185 | | rotated from previous position = 0 degrees |
| 4186 | | atoms outside contour = 4629, contour level = 0.10328 |
| 4187 | | |
| 4188 | | |
| 4189 | | > select clear |
| 4190 | | |
| 4191 | | > select add #2 |
| 4192 | | |
| 4193 | | 2 models selected |
| 4194 | | |
| 4195 | | > fitmap #1 inMap #2 moveWholeMolecules false rotate false |
| 4196 | | |
| 4197 | | Fit molecule 5zf6.pdb (#1) to map cryosparc_P1_J138_004_volume_map_sharp.mrc |
| 4198 | | (#2) using 8555 atoms |
| 4199 | | average map value = 0.1293, steps = 40 |
| 4200 | | shifted from previous position = 0.0141 |
| 4201 | | rotated from previous position = 0 degrees |
| 4202 | | atoms outside contour = 4630, contour level = 0.10328 |
| 4203 | | |
| 4204 | | |
| 4205 | | > fitmap #1 inMap #2 moveWholeMolecules false rotate false |
| 4206 | | |
| 4207 | | Fit molecule 5zf6.pdb (#1) to map cryosparc_P1_J138_004_volume_map_sharp.mrc |
| 4208 | | (#2) using 8555 atoms |
| 4209 | | average map value = 0.1293, steps = 40 |
| 4210 | | shifted from previous position = 0.0156 |
| 4211 | | rotated from previous position = 0 degrees |
| 4212 | | atoms outside contour = 4629, contour level = 0.10328 |
| 4213 | | |
| 4214 | | |
| 4215 | | > fitmap #1 inMap #2 moveWholeMolecules false rotate false |
| 4216 | | |
| 4217 | | Fit molecule 5zf6.pdb (#1) to map cryosparc_P1_J138_004_volume_map_sharp.mrc |
| 4218 | | (#2) using 8555 atoms |
| 4219 | | average map value = 0.1293, steps = 36 |
| 4220 | | shifted from previous position = 0.00098 |
| 4221 | | rotated from previous position = 0 degrees |
| 4222 | | atoms outside contour = 4629, contour level = 0.10328 |
| 4223 | | |
| 4224 | | |
| 4225 | | > fitmap #1 inMap #2 moveWholeMolecules false rotate false |
| 4226 | | |
| 4227 | | Fit molecule 5zf6.pdb (#1) to map cryosparc_P1_J138_004_volume_map_sharp.mrc |
| 4228 | | (#2) using 8555 atoms |
| 4229 | | average map value = 0.1293, steps = 36 |
| 4230 | | shifted from previous position = 0.000935 |
| 4231 | | rotated from previous position = 0 degrees |
| 4232 | | atoms outside contour = 4630, contour level = 0.10328 |
| 4233 | | |
| 4234 | | |
| 4235 | | > fitmap #1 inMap #2 moveWholeMolecules false rotate false |
| 4236 | | |
| 4237 | | Fit molecule 5zf6.pdb (#1) to map cryosparc_P1_J138_004_volume_map_sharp.mrc |
| 4238 | | (#2) using 8555 atoms |
| 4239 | | average map value = 0.1293, steps = 28 |
| 4240 | | shifted from previous position = 0.00984 |
| 4241 | | rotated from previous position = 0 degrees |
| 4242 | | atoms outside contour = 4631, contour level = 0.10328 |
| 4243 | | |
| 4244 | | |
| 4245 | | > fitmap #1 inMap #2 moveWholeMolecules false rotate false |
| 4246 | | |
| 4247 | | Fit molecule 5zf6.pdb (#1) to map cryosparc_P1_J138_004_volume_map_sharp.mrc |
| 4248 | | (#2) using 8555 atoms |
| 4249 | | average map value = 0.1293, steps = 40 |
| 4250 | | shifted from previous position = 0.00416 |
| 4251 | | rotated from previous position = 0 degrees |
| 4252 | | atoms outside contour = 4629, contour level = 0.10328 |
| 4253 | | |
| 4254 | | |
| 4255 | | > fitmap #4 inMap #2 moveWholeMolecules false rotate false |
| 4256 | | |
| 4257 | | Fit molecule 5zf6.pdb (#4) to map cryosparc_P1_J138_004_volume_map_sharp.mrc |
| 4258 | | (#2) using 8555 atoms |
| 4259 | | average map value = 0.111, steps = 40 |
| 4260 | | shifted from previous position = 0.012 |
| 4261 | | rotated from previous position = 0 degrees |
| 4262 | | atoms outside contour = 5072, contour level = 0.10328 |
| 4263 | | |
| 4264 | | |
| 4265 | | > select subtract #2 |
| 4266 | | |
| 4267 | | Nothing selected |
| 4268 | | |
| 4269 | | > fitmap #4 inMap #2 moveWholeMolecules false rotate false |
| 4270 | | |
| 4271 | | Fit molecule 5zf6.pdb (#4) to map cryosparc_P1_J138_004_volume_map_sharp.mrc |
| 4272 | | (#2) using 8555 atoms |
| 4273 | | average map value = 0.111, steps = 28 |
| 4274 | | shifted from previous position = 0.0186 |
| 4275 | | rotated from previous position = 0 degrees |
| 4276 | | atoms outside contour = 5074, contour level = 0.10328 |
| 4277 | | |
| 4278 | | |
| 4279 | | > hide /B ribbons |
| 4280 | | |
| 4281 | | > select clear |
| 4282 | | |
| 4283 | | > show /B ribbons |
| 4284 | | |
| 4285 | | > select :301-310 |
| 4286 | | |
| 4287 | | 704 atoms, 712 bonds, 80 residues, 4 models selected |
| 4288 | | |
| 4289 | | > select :301-3330 |
| 4290 | | |
| 4291 | | 20188 atoms, 20216 bonds, 2848 residues, 4 models selected |
| 4292 | | |
| 4293 | | > select :301-310 |
| 4294 | | |
| 4295 | | 704 atoms, 712 bonds, 80 residues, 4 models selected |
| 4296 | | |
| 4297 | | > select :301-350 |
| 4298 | | |
| 4299 | | 3360 atoms, 3416 bonds, 400 residues, 4 models selected |
| 4300 | | |
| 4301 | | > set bgColor white |
| 4302 | | |
| 4303 | | > lighting soft |
| 4304 | | |
| 4305 | | [Repeated 2 time(s)] |
| 4306 | | |
| 4307 | | > volume #2 color #6d6d6d69 |
| 4308 | | |
| 4309 | | > volume #2 color #b6b6b669 |
| 4310 | | |
| 4311 | | > set bgColor black |
| 4312 | | |
| 4313 | | > close #4 |
| 4314 | | |
| 4315 | | > open |
| 4316 | | > /Users/madhurikanavalli/Desktop/Madhuri/Downloads/hPNP_dimer_5zf6_fit_tetramer2.pdb |
| 4317 | | |
| 4318 | | hPNP_dimer_5zf6_fit_tetramer2.pdb title: |
| 4319 | | Crystal structure of the dimeric human pnpase [more info...] |
| 4320 | | |
| 4321 | | Chain information for hPNP_dimer_5zf6_fit_tetramer2.pdb #4 |
| 4322 | | --- |
| 4323 | | Chain | Description | UniProt |
| 4324 | | A B | polyribonucleotide nucleotidyltransferase 1, mitochondrial | PNPT1_HUMAN 46-669 |
| 4325 | | |
| 4326 | | |
| 4327 | | > fitmap #4 inMap #2 moveWholeMolecules false rotate false |
| 4328 | | |
| 4329 | | Fit molecule hPNP_dimer_5zf6_fit_tetramer2.pdb (#4) to map |
| 4330 | | cryosparc_P1_J138_004_volume_map_sharp.mrc (#2) using 8555 atoms |
| 4331 | | average map value = 0.06485, steps = 92 |
| 4332 | | shifted from previous position = 12 |
| 4333 | | rotated from previous position = 0 degrees |
| 4334 | | atoms outside contour = 6477, contour level = 0.10328 |
| 4335 | | |
| 4336 | | |
| 4337 | | > close #3 |
| 4338 | | |
| 4339 | | > show #!6 models |
| 4340 | | |
| 4341 | | > fitmap #6 inMap #2 moveWholeMolecules false rotate false |
| 4342 | | |
| 4343 | | Fit molecule hPNP_dimer_5zf6_fit_tetramer1.pdb (#6) to map |
| 4344 | | cryosparc_P1_J138_004_volume_map_sharp.mrc (#2) using 8555 atoms |
| 4345 | | average map value = 0.1297, steps = 40 |
| 4346 | | shifted from previous position = 0.0105 |
| 4347 | | rotated from previous position = 0 degrees |
| 4348 | | atoms outside contour = 4642, contour level = 0.10328 |
| 4349 | | |
| 4350 | | |
| 4351 | | > fitmap #6 inMap #2 moveWholeMolecules false rotate false |
| 4352 | | |
| 4353 | | Fit molecule hPNP_dimer_5zf6_fit_tetramer1.pdb (#6) to map |
| 4354 | | cryosparc_P1_J138_004_volume_map_sharp.mrc (#2) using 8555 atoms |
| 4355 | | average map value = 0.1297, steps = 40 |
| 4356 | | shifted from previous position = 0.00663 |
| 4357 | | rotated from previous position = 0 degrees |
| 4358 | | atoms outside contour = 4644, contour level = 0.10328 |
| 4359 | | |
| 4360 | | |
| 4361 | | > select add #6 |
| 4362 | | |
| 4363 | | 9395 atoms, 9472 bonds, 1274 residues, 2 models selected |
| 4364 | | |
| 4365 | | > fitmap #6 inMap #2 moveWholeMolecules false rotate false |
| 4366 | | |
| 4367 | | Fit molecule hPNP_dimer_5zf6_fit_tetramer1.pdb (#6) to map |
| 4368 | | cryosparc_P1_J138_004_volume_map_sharp.mrc (#2) using 8555 atoms |
| 4369 | | average map value = 0.1297, steps = 40 |
| 4370 | | shifted from previous position = 0.0111 |
| 4371 | | rotated from previous position = 0 degrees |
| 4372 | | atoms outside contour = 4642, contour level = 0.10328 |
| 4373 | | |
| 4374 | | |
| 4375 | | > close #4 |
| 4376 | | |
| 4377 | | > open /Users/madhurikanavalli/Downloads/hPNP_dimer_5zf6_fit_tetramer1.pdb |
| 4378 | | |
| 4379 | | hPNP_dimer_5zf6_fit_tetramer1.pdb title: |
| 4380 | | Crystal structure of the dimeric human pnpase [more info...] |
| 4381 | | |
| 4382 | | Chain information for hPNP_dimer_5zf6_fit_tetramer1.pdb #3 |
| 4383 | | --- |
| 4384 | | Chain | Description | UniProt |
| 4385 | | A B | polyribonucleotide nucleotidyltransferase 1, mitochondrial | PNPT1_HUMAN 46-669 |
| 4386 | | |
| 4387 | | |
| 4388 | | > view matrix models |
| 4389 | | > #1,0.994,-0.094744,-0.054626,186.44,0.077109,0.25294,0.9644,196.21,-0.077554,-0.96283,0.25873,175.8,#6,0.99794,0.050158,0.040032,-19.038,-0.032417,0.93235,-0.36009,102.59,-0.055385,0.35805,0.93206,-81.741 |
| 4390 | | |
| 4391 | | > undo |
| 4392 | | |
| 4393 | | > hide #!6 models |
| 4394 | | |
| 4395 | | > select subtract #6 |
| 4396 | | |
| 4397 | | 840 atoms, 854 bonds, 100 residues, 1 model selected |
| 4398 | | |
| 4399 | | > hide #!1 models |
| 4400 | | |
| 4401 | | > select add #1 |
| 4402 | | |
| 4403 | | 8555 atoms, 8618 bonds, 1174 residues, 1 model selected |
| 4404 | | |
| 4405 | | > select subtract #1 |
| 4406 | | |
| 4407 | | Nothing selected |
| 4408 | | |
| 4409 | | > select add #3 |
| 4410 | | |
| 4411 | | 8555 atoms, 8618 bonds, 1174 residues, 1 model selected |
| 4412 | | |
| 4413 | | > view matrix models #3,1,0,0,1.8097,0,1,0,0.23566,0,0,1,-36.997 |
| 4414 | | |
| 4415 | | > view matrix models #3,1,0,0,35.394,0,1,0,-3.855,0,0,1,-64.467 |
| 4416 | | |
| 4417 | | > view matrix models #3,1,0,0,29.376,0,1,0,4.2566,0,0,1,1.29 |
| 4418 | | |
| 4419 | | > fitmap #3 inMap #2 moveWholeMolecules false rotate false |
| 4420 | | |
| 4421 | | Fit molecule hPNP_dimer_5zf6_fit_tetramer1.pdb (#3) to map |
| 4422 | | cryosparc_P1_J138_004_volume_map_sharp.mrc (#2) using 8555 atoms |
| 4423 | | average map value = 0.0719, steps = 92 |
| 4424 | | shifted from previous position = 9.37 |
| 4425 | | rotated from previous position = 0 degrees |
| 4426 | | atoms outside contour = 6159, contour level = 0.10328 |
| 4427 | | |
| 4428 | | |
| 4429 | | > view matrix models #3,1,0,0,31.644,0,1,0,5.3303,0,0,1,3.0727 |
| 4430 | | |
| 4431 | | > fitmap #3 inMap #2 moveWholeMolecules false rotate false |
| 4432 | | |
| 4433 | | Fit molecule hPNP_dimer_5zf6_fit_tetramer1.pdb (#3) to map |
| 4434 | | cryosparc_P1_J138_004_volume_map_sharp.mrc (#2) using 8555 atoms |
| 4435 | | average map value = 0.07189, steps = 48 |
| 4436 | | shifted from previous position = 3.09 |
| 4437 | | rotated from previous position = 0 degrees |
| 4438 | | atoms outside contour = 6159, contour level = 0.10328 |
| 4439 | | |
| 4440 | | |
| 4441 | | > view matrix models #3,1,0,0,34.027,0,1,0,1.7493,0,0,1,4.222 |
| 4442 | | |
| 4443 | | > ui mousemode right "rotate selected models" |
| 4444 | | |
| 4445 | | > view matrix models |
| 4446 | | > #3,0.927,0.099914,0.3615,-47.503,-0.090663,0.99497,-0.042509,27.476,-0.36393,0.0066319,0.9314,81.127 |
| 4447 | | |
| 4448 | | > fitmap #3 inMap #2 moveWholeMolecules false |
| 4449 | | |
| 4450 | | Fit molecule hPNP_dimer_5zf6_fit_tetramer1.pdb (#3) to map |
| 4451 | | cryosparc_P1_J138_004_volume_map_sharp.mrc (#2) using 8555 atoms |
| 4452 | | average map value = 0.1263, steps = 2000 |
| 4453 | | shifted from previous position = 3.7 |
| 4454 | | rotated from previous position = 7.87 degrees |
| 4455 | | atoms outside contour = 4616, contour level = 0.10328 |
| 4456 | | |
| 4457 | | |
| 4458 | | > fitmap #3 inMap #2 moveWholeMolecules false |
| 4459 | | |
| 4460 | | Fit molecule hPNP_dimer_5zf6_fit_tetramer1.pdb (#3) to map |
| 4461 | | cryosparc_P1_J138_004_volume_map_sharp.mrc (#2) using 8555 atoms |
| 4462 | | average map value = 0.1261, steps = 2000 |
| 4463 | | shifted from previous position = 0.241 |
| 4464 | | rotated from previous position = 0.799 degrees |
| 4465 | | atoms outside contour = 4569, contour level = 0.10328 |
| 4466 | | |
| 4467 | | |
| 4468 | | > volume #2 color #9c9c9c69 |
| 4469 | | |
| 4470 | | > volume #2 color #8e8e8e69 |
| 4471 | | |
| 4472 | | > volume #2 color #4c4c4c69 |
| 4473 | | |
| 4474 | | > volume #2 color #ffffff69 |
| 4475 | | |
| 4476 | | > volume #2 color #adadad69 |
| 4477 | | |
| 4478 | | > color #3 #c76be1ff |
| 4479 | | |
| 4480 | | > color #3 #ba49e1ff |
| 4481 | | |
| 4482 | | > color #3 #e119e0ff |
| 4483 | | |
| 4484 | | > color #3 #e121bdff |
| 4485 | | |
| 4486 | | > select subtract #3 |
| 4487 | | |
| 4488 | | Nothing selected |
| 4489 | | |
| 4490 | | > fitmap #3 inMap #2 |
| 4491 | | |
| 4492 | | Fit molecule hPNP_dimer_5zf6_fit_tetramer1.pdb (#3) to map |
| 4493 | | cryosparc_P1_J138_004_volume_map_sharp.mrc (#2) using 8555 atoms |
| 4494 | | average map value = 0.1249, steps = 2000 |
| 4495 | | shifted from previous position = 0.637 |
| 4496 | | rotated from previous position = 1.01 degrees |
| 4497 | | atoms outside contour = 4588, contour level = 0.10328 |
| 4498 | | |
| 4499 | | Position of hPNP_dimer_5zf6_fit_tetramer1.pdb (#3) relative to |
| 4500 | | cryosparc_P1_J138_004_volume_map_sharp.mrc (#2) coordinates: |
| 4501 | | Matrix rotation and translation |
| 4502 | | 0.99026797 0.07877011 0.11473715 -66.08205858 |
| 4503 | | -0.08429553 0.99546348 0.04412174 -36.13256533 |
| 4504 | | -0.11074116 -0.05336418 0.99241557 6.84643690 |
| 4505 | | Axis -0.33063295 0.76473153 -0.55305292 |
| 4506 | | Axis point -27.14208933 0.00000000 601.21610338 |
| 4507 | | Rotation angle (degrees) 8.47762549 |
| 4508 | | Shift along axis -9.56924816 |
| 4509 | | |
| 4510 | | |
| 4511 | | > hide #!3 models |
| 4512 | | |
| 4513 | | > show #!1 models |
| 4514 | | |
| 4515 | | > select add #1 |
| 4516 | | |
| 4517 | | 8555 atoms, 8618 bonds, 1174 residues, 1 model selected |
| 4518 | | |
| 4519 | | > show #!3 models |
| 4520 | | |
| 4521 | | > select add #3 |
| 4522 | | |
| 4523 | | 17110 atoms, 17236 bonds, 2348 residues, 2 models selected |
| 4524 | | |
| 4525 | | > color #3 #24cbd8ff |
| 4526 | | |
| 4527 | | > color #3 #b4d827ff |
| 4528 | | |
| 4529 | | > select subtract #3 |
| 4530 | | |
| 4531 | | 8555 atoms, 8618 bonds, 1174 residues, 1 model selected |
| 4532 | | |
| 4533 | | > hide #!3 models |
| 4534 | | |
| 4535 | | > hide #!1 models |
| 4536 | | |
| 4537 | | > show #!1 models |
| 4538 | | |
| 4539 | | > color #3 #4264d8ff |
| 4540 | | |
| 4541 | | > color #1 #6d61d7ff |
| 4542 | | |
| 4543 | | > select subtract #1 |
| 4544 | | |
| 4545 | | Nothing selected |
| 4546 | | |
| 4547 | | > select #1/B |
| 4548 | | |
| 4549 | | 4227 atoms, 4261 bonds, 577 residues, 1 model selected |
| 4550 | | |
| 4551 | | > fitmap #1 inMap #2 moveWholeMolecules false |
| 4552 | | |
| 4553 | | Fit molecule 5zf6.pdb (#1) to map cryosparc_P1_J138_004_volume_map_sharp.mrc |
| 4554 | | (#2) using 8555 atoms |
| 4555 | | average map value = 0.1297, steps = 340 |
| 4556 | | shifted from previous position = 0.209 |
| 4557 | | rotated from previous position = 0.503 degrees |
| 4558 | | atoms outside contour = 4642, contour level = 0.10328 |
| 4559 | | |
| 4560 | | |
| 4561 | | > fitmap #1 inMap #2 moveWholeMolecules false |
| 4562 | | |
| 4563 | | Fit molecule 5zf6.pdb (#1) to map cryosparc_P1_J138_004_volume_map_sharp.mrc |
| 4564 | | (#2) using 8555 atoms |
| 4565 | | average map value = 0.1297, steps = 504 |
| 4566 | | shifted from previous position = 0.0115 |
| 4567 | | rotated from previous position = 0.0127 degrees |
| 4568 | | atoms outside contour = 4645, contour level = 0.10328 |
| 4569 | | |
| 4570 | | |
| 4571 | | > fitmap #1 inMap #2 moveWholeMolecules false |
| 4572 | | |
| 4573 | | Fit molecule 5zf6.pdb (#1) to map cryosparc_P1_J138_004_volume_map_sharp.mrc |
| 4574 | | (#2) using 8555 atoms |
| 4575 | | average map value = 0.1297, steps = 348 |
| 4576 | | shifted from previous position = 0.00671 |
| 4577 | | rotated from previous position = 0.0114 degrees |
| 4578 | | atoms outside contour = 4646, contour level = 0.10328 |
| 4579 | | |
| 4580 | | |
| 4581 | | > select add #1 |
| 4582 | | |
| 4583 | | 8555 atoms, 8618 bonds, 1174 residues, 1 model selected |
| 4584 | | |
| 4585 | | > select subtract #1 |
| 4586 | | |
| 4587 | | Nothing selected |
| 4588 | | |
| 4589 | | > color #1 #2322d7ff |
| 4590 | | |
| 4591 | | > color #1 #304bd7ff |
| 4592 | | |
| 4593 | | > color #1 #405cd7ff |
| 4594 | | |
| 4595 | | > select #1/A-B:293 |
| 4596 | | |
| 4597 | | 18 atoms, 16 bonds, 2 residues, 1 model selected |
| 4598 | | |
| 4599 | | > select #1/A-B:293-367 |
| 4600 | | |
| 4601 | | 1256 atoms, 1276 bonds, 150 residues, 1 model selected |
| 4602 | | |
| 4603 | | > hide /chain bonds |
| 4604 | | |
| 4605 | | [Repeated 1 time(s)] |
| 4606 | | |
| 4607 | | > fitmap #1 inMap #2 moveWholeMolecules false |
| 4608 | | |
| 4609 | | Fit molecule 5zf6.pdb (#1) to map cryosparc_P1_J138_004_volume_map_sharp.mrc |
| 4610 | | (#2) using 8555 atoms |
| 4611 | | average map value = 0.129, steps = 2000 |
| 4612 | | shifted from previous position = 0.217 |
| 4613 | | rotated from previous position = 0.341 degrees |
| 4614 | | atoms outside contour = 4649, contour level = 0.10328 |
| 4615 | | |
| 4616 | | |
| 4617 | | > fitmap #1 inMap #2 moveWholeMolecules false |
| 4618 | | |
| 4619 | | Fit molecule 5zf6.pdb (#1) to map cryosparc_P1_J138_004_volume_map_sharp.mrc |
| 4620 | | (#2) using 8555 atoms |
| 4621 | | average map value = 0.1275, steps = 2000 |
| 4622 | | shifted from previous position = 0.536 |
| 4623 | | rotated from previous position = 0.764 degrees |
| 4624 | | atoms outside contour = 4674, contour level = 0.10328 |
| 4625 | | |
| 4626 | | |
| 4627 | | > select add #6 |
| 4628 | | |
| 4629 | | 9811 atoms, 9894 bonds, 1324 residues, 2 models selected |
| 4630 | | |
| 4631 | | > show #!6 models |
| 4632 | | |
| 4633 | | > hide #!6 models |
| 4634 | | |
| 4635 | | > select subtract #6 |
| 4636 | | |
| 4637 | | 1256 atoms, 1276 bonds, 150 residues, 1 model selected |
| 4638 | | |
| 4639 | | > select add #3 |
| 4640 | | |
| 4641 | | 9811 atoms, 9894 bonds, 1324 residues, 2 models selected |
| 4642 | | |
| 4643 | | > show #!3 models |
| 4644 | | |
| 4645 | | > show #!6 models |
| 4646 | | |
| 4647 | | > select add #6 |
| 4648 | | |
| 4649 | | 18366 atoms, 18512 bonds, 2498 residues, 3 models selected |
| 4650 | | |
| 4651 | | > hide #!3 models |
| 4652 | | |
| 4653 | | > select subtract #3 |
| 4654 | | |
| 4655 | | 9811 atoms, 9894 bonds, 1324 residues, 2 models selected |
| 4656 | | |
| 4657 | | > select add #1 |
| 4658 | | |
| 4659 | | 17110 atoms, 17236 bonds, 2348 residues, 2 models selected |
| 4660 | | |
| 4661 | | > view matrix models |
| 4662 | | > #1,0.96064,0.23479,0.14849,209.24,-0.19007,0.16566,0.96769,190.25,0.2026,-0.95783,0.20377,213.18,#6,0.92816,0.21046,-0.30695,60.885,-0.29394,0.92042,-0.25774,126.04,0.22828,0.32945,0.91616,-88.788 |
| 4663 | | |
| 4664 | | > undo |
| 4665 | | |
| 4666 | | > select subtract #1 |
| 4667 | | |
| 4668 | | 8555 atoms, 8618 bonds, 1174 residues, 1 model selected |
| 4669 | | |
| 4670 | | > hide #!1 models |
| 4671 | | |
| 4672 | | > view matrix models |
| 4673 | | > #6,-0.27804,-0.71665,-0.63961,531.76,-0.24176,0.69664,-0.67546,247.87,0.92965,-0.033174,-0.36695,123.49 |
| 4674 | | |
| 4675 | | > view matrix models |
| 4676 | | > #6,-0.80854,-0.29339,-0.51009,522.64,0.17861,0.70359,-0.68779,171.01,0.56068,-0.64722,-0.51647,340.29 |
| 4677 | | |
| 4678 | | > ui mousemode right "translate selected models" |
| 4679 | | |
| 4680 | | > view matrix models |
| 4681 | | > #6,-0.80854,-0.29339,-0.51009,480.38,0.17861,0.70359,-0.68779,178.26,0.56068,-0.64722,-0.51647,288.18 |
| 4682 | | |
| 4683 | | > fitmap #6 inMap #2 moveWholeMolecules false |
| 4684 | | |
| 4685 | | Fit molecule hPNP_dimer_5zf6_fit_tetramer1.pdb (#6) to map |
| 4686 | | cryosparc_P1_J138_004_volume_map_sharp.mrc (#2) using 8555 atoms |
| 4687 | | average map value = 0.04021, steps = 244 |
| 4688 | | shifted from previous position = 22.5 |
| 4689 | | rotated from previous position = 9.8 degrees |
| 4690 | | atoms outside contour = 7262, contour level = 0.10328 |
| 4691 | | |
| 4692 | | |
| 4693 | | > ui mousemode right "rotate selected models" |
| 4694 | | |
| 4695 | | > view matrix models |
| 4696 | | > #6,-0.35639,-0.29895,-0.88522,483.61,-0.004137,0.94793,-0.31846,84.725,0.93433,-0.10983,-0.33907,84.393 |
| 4697 | | |
| 4698 | | > view matrix models |
| 4699 | | > #6,-0.25916,-0.51199,-0.81897,494.96,0.10101,0.82892,-0.55017,138.34,0.96054,-0.22531,-0.16311,65.902 |
| 4700 | | |
| 4701 | | > fitmap #6 inMap #2 moveWholeMolecules false |
| 4702 | | |
| 4703 | | Fit molecule hPNP_dimer_5zf6_fit_tetramer1.pdb (#6) to map |
| 4704 | | cryosparc_P1_J138_004_volume_map_sharp.mrc (#2) using 8555 atoms |
| 4705 | | average map value = 0.04682, steps = 180 |
| 4706 | | shifted from previous position = 9.55 |
| 4707 | | rotated from previous position = 14.6 degrees |
| 4708 | | atoms outside contour = 7079, contour level = 0.10328 |
| 4709 | | |
| 4710 | | |
| 4711 | | > fitmap #6 inMap #2 |
| 4712 | | |
| 4713 | | Fit molecule hPNP_dimer_5zf6_fit_tetramer1.pdb (#6) to map |
| 4714 | | cryosparc_P1_J138_004_volume_map_sharp.mrc (#2) using 8555 atoms |
| 4715 | | average map value = 0.04682, steps = 44 |
| 4716 | | shifted from previous position = 0.0919 |
| 4717 | | rotated from previous position = 0.204 degrees |
| 4718 | | atoms outside contour = 7090, contour level = 0.10328 |
| 4719 | | |
| 4720 | | Position of hPNP_dimer_5zf6_fit_tetramer1.pdb (#6) relative to |
| 4721 | | cryosparc_P1_J138_004_volume_map_sharp.mrc (#2) coordinates: |
| 4722 | | Matrix rotation and translation |
| 4723 | | -0.49668241 -0.45211172 -0.74087892 460.77188640 |
| 4724 | | 0.15262355 0.79481607 -0.58734441 94.44318983 |
| 4725 | | 0.85440777 -0.40479921 -0.32576827 117.80701656 |
| 4726 | | Axis 0.10639077 -0.92976301 0.35245106 |
| 4727 | | Axis point 200.17990455 0.00000000 217.14121089 |
| 4728 | | Rotation angle (degrees) 120.91843476 |
| 4729 | | Shift along axis 2.73329794 |
| 4730 | | |
| 4731 | | |
| 4732 | | > view matrix models |
| 4733 | | > #6,-0.52907,-0.0047689,-0.84857,450.01,0.53325,0.77601,-0.33683,33.426,0.6601,-0.6307,-0.40802,241.83 |
| 4734 | | |
| 4735 | | > ui mousemode right "translate selected models" |
| 4736 | | |
| 4737 | | > view matrix models |
| 4738 | | > #6,-0.52907,-0.0047689,-0.84857,424.69,0.53325,0.77601,-0.33683,-64.127,0.6601,-0.6307,-0.40802,233.02 |
| 4739 | | |
| 4740 | | > view matrix models |
| 4741 | | > #6,-0.52907,-0.0047689,-0.84857,427.56,0.53325,0.77601,-0.33683,-69.035,0.6601,-0.6307,-0.40802,240.81 |
| 4742 | | |
| 4743 | | > ui mousemode right "rotate selected models" |
| 4744 | | |
| 4745 | | > view matrix models |
| 4746 | | > #6,-0.76339,-0.51264,-0.39298,467.55,-0.13914,0.72461,-0.67496,120.5,0.63077,-0.46058,-0.62449,258.25 |
| 4747 | | |
| 4748 | | > view matrix models |
| 4749 | | > #6,-0.86546,-0.26374,-0.42593,444.28,0.16092,0.65879,-0.73491,96.05,0.47442,-0.70458,-0.52772,309.8 |
| 4750 | | |
| 4751 | | > view matrix models |
| 4752 | | > #6,-0.28971,-0.90683,-0.30614,446.12,-0.56931,0.42039,-0.70651,254.69,0.76939,-0.030393,-0.63806,157.46 |
| 4753 | | |
| 4754 | | > view matrix models |
| 4755 | | > #6,0.071191,-0.22599,-0.97153,396.1,-0.32791,0.91456,-0.23676,25.444,0.94202,0.33543,-0.0089961,-69.223 |
| 4756 | | |
| 4757 | | > view matrix models |
| 4758 | | > #6,-0.62244,0.01769,-0.78247,425.02,-0.32291,0.90489,0.27732,-79.49,0.71295,0.42528,-0.55752,64.459 |
| 4759 | | |
| 4760 | | > view matrix models |
| 4761 | | > #6,-0.22541,0.34331,-0.91177,325.38,-0.19676,0.90053,0.38772,-122.1,0.95419,0.26679,-0.13544,-32.26 |
| 4762 | | |
| 4763 | | > view matrix models |
| 4764 | | > #6,-0.41827,-0.28212,-0.8634,464.59,-0.64872,0.75811,0.066552,44.931,0.63578,0.58794,-0.50012,34.704 |
| 4765 | | |
| 4766 | | > view matrix models |
| 4767 | | > #6,-0.25107,0.40226,-0.88043,312.05,-0.2867,0.83785,0.46456,-111.4,0.92454,0.36905,-0.095032,-54.949 |
| 4768 | | |
| 4769 | | > view matrix models |
| 4770 | | > #6,-0.76906,0.13581,-0.62458,394.31,-0.1572,0.90696,0.39079,-130.42,0.61954,0.39873,-0.67616,109.21 |
| 4771 | | |
| 4772 | | > view matrix models |
| 4773 | | > #6,-0.98343,0.03782,-0.1773,355.7,-0.074325,0.80792,0.58458,-165.32,0.16536,0.58807,-0.79172,171.87 |
| 4774 | | |
| 4775 | | > view matrix models |
| 4776 | | > #6,-0.39477,0.68819,-0.60873,225.86,0.36199,0.72545,0.58539,-221.49,0.84446,0.010739,-0.5355,116.29 |
| 4777 | | |
| 4778 | | > view matrix models |
| 4779 | | > #6,-0.79966,0.075061,-0.59575,404.81,-0.040684,0.9831,0.17847,-120.08,0.59908,0.16695,-0.78309,178.16 |
| 4780 | | |
| 4781 | | > fitmap #6 inMap #2 |
| 4782 | | |
| 4783 | | Fit molecule hPNP_dimer_5zf6_fit_tetramer1.pdb (#6) to map |
| 4784 | | cryosparc_P1_J138_004_volume_map_sharp.mrc (#2) using 8555 atoms |
| 4785 | | average map value = -0.0005825, steps = 96 |
| 4786 | | shifted from previous position = 3.28 |
| 4787 | | rotated from previous position = 3.03 degrees |
| 4788 | | atoms outside contour = 8555, contour level = 0.10328 |
| 4789 | | |
| 4790 | | Position of hPNP_dimer_5zf6_fit_tetramer1.pdb (#6) relative to |
| 4791 | | cryosparc_P1_J138_004_volume_map_sharp.mrc (#2) coordinates: |
| 4792 | | Matrix rotation and translation |
| 4793 | | -0.92490882 -0.02938852 -0.37905143 356.59797481 |
| 4794 | | -0.08084042 0.98941082 0.12054485 -153.92939959 |
| 4795 | | 0.37149495 0.14213567 -0.91749057 213.28677900 |
| 4796 | | Axis 0.02868764 -0.99724791 -0.06836393 |
| 4797 | | Axis point 154.19781165 0.00000000 146.42433529 |
| 4798 | | Rotation angle (degrees) 157.89477035 |
| 4799 | | Shift along axis 149.15460281 |
| 4800 | | |
| 4801 | | |
| 4802 | | > fitmap #6 inMap #2 |
| 4803 | | |
| 4804 | | Fit molecule hPNP_dimer_5zf6_fit_tetramer1.pdb (#6) to map |
| 4805 | | cryosparc_P1_J138_004_volume_map_sharp.mrc (#2) using 8555 atoms |
| 4806 | | average map value = -0.0005823, steps = 28 |
| 4807 | | shifted from previous position = 0.00579 |
| 4808 | | rotated from previous position = 0.00269 degrees |
| 4809 | | atoms outside contour = 8555, contour level = 0.10328 |
| 4810 | | |
| 4811 | | Position of hPNP_dimer_5zf6_fit_tetramer1.pdb (#6) relative to |
| 4812 | | cryosparc_P1_J138_004_volume_map_sharp.mrc (#2) coordinates: |
| 4813 | | Matrix rotation and translation |
| 4814 | | -0.92492308 -0.02936526 -0.37901843 356.58713815 |
| 4815 | | -0.08080731 0.98941435 0.12053802 -153.93925469 |
| 4816 | | 0.37146665 0.14211586 -0.91750510 213.29930744 |
| 4817 | | Axis 0.02867275 -0.99724885 -0.06835648 |
| 4818 | | Axis point 154.19422266 0.00000000 146.42639014 |
| 4819 | | Rotation angle (degrees) 157.89669311 |
| 4820 | | Shift along axis 149.15968848 |
| 4821 | | |
| 4822 | | |
| 4823 | | > ui mousemode right "translate selected models" |
| 4824 | | |
| 4825 | | > view matrix models |
| 4826 | | > #6,-0.80444,0.039308,-0.59273,388.5,-0.090985,0.97788,0.18834,-22.775,0.58702,0.20543,-0.78307,177.39 |
| 4827 | | |
| 4828 | | > volume #2 level 0.0888 |
| 4829 | | |
| 4830 | | > volume #2 level 0.09242 |
| 4831 | | |
| 4832 | | > fitmap #6 inMap #2 |
| 4833 | | |
| 4834 | | Fit molecule hPNP_dimer_5zf6_fit_tetramer1.pdb (#6) to map |
| 4835 | | cryosparc_P1_J138_004_volume_map_sharp.mrc (#2) using 8555 atoms |
| 4836 | | average map value = 0.03454, steps = 280 |
| 4837 | | shifted from previous position = 32.1 |
| 4838 | | rotated from previous position = 34.8 degrees |
| 4839 | | atoms outside contour = 7410, contour level = 0.092417 |
| 4840 | | |
| 4841 | | Position of hPNP_dimer_5zf6_fit_tetramer1.pdb (#6) relative to |
| 4842 | | cryosparc_P1_J138_004_volume_map_sharp.mrc (#2) coordinates: |
| 4843 | | Matrix rotation and translation |
| 4844 | | -0.90947492 -0.41561998 0.01074203 352.97733471 |
| 4845 | | -0.39427535 0.87038984 0.29490417 -29.46763984 |
| 4846 | | -0.13191783 0.26397263 -0.95546645 264.47171436 |
| 4847 | | Axis -0.20966712 0.96700906 0.14468300 |
| 4848 | | Axis point 178.52191521 0.00000000 127.95705739 |
| 4849 | | Rotation angle (degrees) 175.76982332 |
| 4850 | | Shift along axis -64.23865431 |
| 4851 | | |
| 4852 | | |
| 4853 | | > view matrix models |
| 4854 | | > #6,-0.92581,-0.30774,-0.21947,427.56,-0.3699,0.85706,0.35863,24.591,0.077733,0.41321,-0.90731,235.42 |
| 4855 | | |
| 4856 | | > view matrix models |
| 4857 | | > #6,-0.92581,-0.30774,-0.21947,445.36,-0.3699,0.85706,0.35863,22.611,0.077733,0.41321,-0.90731,226.52 |
| 4858 | | |
| 4859 | | > fitmap #6 inMap #2 |
| 4860 | | |
| 4861 | | Fit molecule hPNP_dimer_5zf6_fit_tetramer1.pdb (#6) to map |
| 4862 | | cryosparc_P1_J138_004_volume_map_sharp.mrc (#2) using 8555 atoms |
| 4863 | | average map value = 0.06286, steps = 200 |
| 4864 | | shifted from previous position = 6.44 |
| 4865 | | rotated from previous position = 25.2 degrees |
| 4866 | | atoms outside contour = 6186, contour level = 0.092417 |
| 4867 | | |
| 4868 | | Position of hPNP_dimer_5zf6_fit_tetramer1.pdb (#6) relative to |
| 4869 | | cryosparc_P1_J138_004_volume_map_sharp.mrc (#2) coordinates: |
| 4870 | | Matrix rotation and translation |
| 4871 | | -0.94057101 -0.15322448 -0.30306507 389.01736554 |
| 4872 | | -0.21329908 0.96098057 0.17612448 -31.15370261 |
| 4873 | | 0.26425307 0.23030108 -0.93655311 208.49710154 |
| 4874 | | Axis 0.09453969 -0.98998612 -0.10483186 |
| 4875 | | Axis point 177.25741183 0.00000000 134.32900383 |
| 4876 | | Rotation angle (degrees) 163.34977424 |
| 4877 | | Shift along axis 45.76217553 |
| 4878 | | |
| 4879 | | |
| 4880 | | > select subtract #6 |
| 4881 | | |
| 4882 | | Nothing selected |
| 4883 | | |
| 4884 | | > ui mousemode right "rotate selected models" |
| 4885 | | |
| 4886 | | > select add #6 |
| 4887 | | |
| 4888 | | 8555 atoms, 8618 bonds, 1174 residues, 1 model selected |
| 4889 | | |
| 4890 | | > view matrix models |
| 4891 | | > #6,-0.76191,-0.0097647,-0.64761,446.5,-0.20035,0.9544,0.22132,8.908,0.61591,0.29837,-0.72912,128.23 |
| 4892 | | |
| 4893 | | > fitmap #6 inMap #2 |
| 4894 | | |
| 4895 | | Fit molecule hPNP_dimer_5zf6_fit_tetramer1.pdb (#6) to map |
| 4896 | | cryosparc_P1_J138_004_volume_map_sharp.mrc (#2) using 8555 atoms |
| 4897 | | average map value = 0.111, steps = 324 |
| 4898 | | shifted from previous position = 1.84 |
| 4899 | | rotated from previous position = 10.5 degrees |
| 4900 | | atoms outside contour = 4725, contour level = 0.092417 |
| 4901 | | |
| 4902 | | Position of hPNP_dimer_5zf6_fit_tetramer1.pdb (#6) relative to |
| 4903 | | cryosparc_P1_J138_004_volume_map_sharp.mrc (#2) coordinates: |
| 4904 | | Matrix rotation and translation |
| 4905 | | -0.94065942 0.02845456 -0.33815704 363.23421540 |
| 4906 | | -0.02966661 0.98576805 0.16547275 -62.67392798 |
| 4907 | | 0.33805287 0.16568547 -0.92642786 207.49334626 |
| 4908 | | Axis 0.00031343 -0.99632647 -0.08563563 |
| 4909 | | Axis point 163.06221544 0.00000000 138.31429213 |
| 4910 | | Rotation angle (degrees) 160.16262663 |
| 4911 | | Shift along axis 44.78871788 |
| 4912 | | |
| 4913 | | |
| 4914 | | > ui mousemode right "translate selected models" |
| 4915 | | |
| 4916 | | > ui mousemode right "rotate selected models" |
| 4917 | | |
| 4918 | | > view matrix models |
| 4919 | | > #6,-0.64462,0.060699,-0.76209,438.99,-0.16555,0.96211,0.21666,3.6667,0.74636,0.26583,-0.61015,89.225 |
| 4920 | | |
| 4921 | | > fitmap #6 inMap #2 |
| 4922 | | |
| 4923 | | Fit molecule hPNP_dimer_5zf6_fit_tetramer1.pdb (#6) to map |
| 4924 | | cryosparc_P1_J138_004_volume_map_sharp.mrc (#2) using 8555 atoms |
| 4925 | | average map value = 0.111, steps = 232 |
| 4926 | | shifted from previous position = 1.12 |
| 4927 | | rotated from previous position = 16.6 degrees |
| 4928 | | atoms outside contour = 4726, contour level = 0.092417 |
| 4929 | | |
| 4930 | | Position of hPNP_dimer_5zf6_fit_tetramer1.pdb (#6) relative to |
| 4931 | | cryosparc_P1_J138_004_volume_map_sharp.mrc (#2) coordinates: |
| 4932 | | Matrix rotation and translation |
| 4933 | | -0.94065481 0.02863395 -0.33815474 363.19518707 |
| 4934 | | -0.02945052 0.98578726 0.16539693 -62.69088648 |
| 4935 | | 0.33808460 0.16554025 -0.92644224 207.53053559 |
| 4936 | | Axis 0.00021116 -0.99633143 -0.08557825 |
| 4937 | | Axis point 163.04229456 0.00000000 138.32992186 |
| 4938 | | Rotation angle (degrees) 160.16182981 |
| 4939 | | Shift along axis 44.77749212 |
| 4940 | | |
| 4941 | | |
| 4942 | | > view matrix models |
| 4943 | | > #6,-0.88604,0.038516,-0.462,420.9,-0.03934,0.9867,0.15771,-9.4885,0.46193,0.15791,-0.87275,210.25 |
| 4944 | | |
| 4945 | | > fitmap #6 inMap #2 |
| 4946 | | |
| 4947 | | Fit molecule hPNP_dimer_5zf6_fit_tetramer1.pdb (#6) to map |
| 4948 | | cryosparc_P1_J138_004_volume_map_sharp.mrc (#2) using 8555 atoms |
| 4949 | | average map value = 0.111, steps = 192 |
| 4950 | | shifted from previous position = 0.498 |
| 4951 | | rotated from previous position = 8.14 degrees |
| 4952 | | atoms outside contour = 4728, contour level = 0.092417 |
| 4953 | | |
| 4954 | | Position of hPNP_dimer_5zf6_fit_tetramer1.pdb (#6) relative to |
| 4955 | | cryosparc_P1_J138_004_volume_map_sharp.mrc (#2) coordinates: |
| 4956 | | Matrix rotation and translation |
| 4957 | | -0.94066665 0.02855601 -0.33812838 363.20627610 |
| 4958 | | -0.02957366 0.98576223 0.16552410 -62.69813440 |
| 4959 | | 0.33804090 0.16570270 -0.92642915 207.49987033 |
| 4960 | | Axis 0.00026316 -0.99632498 -0.08565318 |
| 4961 | | Axis point 163.05029791 0.00000000 138.31495884 |
| 4962 | | Rotation angle (degrees) 160.16383770 |
| 4963 | | Shift along axis 44.79027516 |
| 4964 | | |
| 4965 | | |
| 4966 | | > view matrix models |
| 4967 | | > #6,-0.72409,0.17019,-0.66837,412.13,-0.025472,0.96182,0.2725,-30.744,0.68923,0.21434,-0.69211,125.17 |
| 4968 | | |
| 4969 | | > fitmap #6 inMap #2 moveWholeMolecules false |
| 4970 | | |
| 4971 | | Fit molecule hPNP_dimer_5zf6_fit_tetramer1.pdb (#6) to map |
| 4972 | | cryosparc_P1_J138_004_volume_map_sharp.mrc (#2) using 8555 atoms |
| 4973 | | average map value = 0.111, steps = 776 |
| 4974 | | shifted from previous position = 0.661 |
| 4975 | | rotated from previous position = 9.75 degrees |
| 4976 | | atoms outside contour = 4722, contour level = 0.092417 |
| 4977 | | |
| 4978 | | |
| 4979 | | > select subtract #6 |
| 4980 | | |
| 4981 | | Nothing selected |
| 4982 | | |
| 4983 | | > select add #6 |
| 4984 | | |
| 4985 | | 8555 atoms, 8618 bonds, 1174 residues, 1 model selected |
| 4986 | | |
| 4987 | | > view matrix models |
| 4988 | | > #6,-0.76187,-0.15221,-0.6296,471.01,-0.29458,0.94708,0.12751,45.668,0.57687,0.28261,-0.76639,145.88 |
| 4989 | | |
| 4990 | | > ui mousemode right "translate selected models" |
| 4991 | | |
| 4992 | | > view matrix models |
| 4993 | | > #6,-0.76187,-0.15221,-0.6296,471.34,-0.29458,0.94708,0.12751,43.678,0.57687,0.28261,-0.76639,146.42 |
| 4994 | | |
| 4995 | | > fitmap #6 inMap #2 moveWholeMolecules false |
| 4996 | | |
| 4997 | | Fit molecule hPNP_dimer_5zf6_fit_tetramer1.pdb (#6) to map |
| 4998 | | cryosparc_P1_J138_004_volume_map_sharp.mrc (#2) using 8555 atoms |
| 4999 | | average map value = 0.06236, steps = 84 |
| 5000 | | shifted from previous position = 2.22 |
| 5001 | | rotated from previous position = 3.59 degrees |
| 5002 | | atoms outside contour = 6255, contour level = 0.092417 |
| 5003 | | |
| 5004 | | |
| 5005 | | > select subtract #6 |
| 5006 | | |
| 5007 | | Nothing selected |
| 5008 | | |
| 5009 | | > ui mousemode right "rotate selected models" |
| 5010 | | |
| 5011 | | > select add #6 |
| 5012 | | |
| 5013 | | 8555 atoms, 8618 bonds, 1174 residues, 1 model selected |
| 5014 | | |
| 5015 | | > view matrix models |
| 5016 | | > #6,-0.6777,0.059786,-0.73291,439.37,-0.22546,0.93179,0.28449,3.074,0.69992,0.35804,-0.61799,81.912 |
| 5017 | | |
| 5018 | | > ui mousemode right "translate selected models" |
| 5019 | | |
| 5020 | | > view matrix models |
| 5021 | | > #6,-0.6777,0.059786,-0.73291,439.44,-0.22546,0.93179,0.28449,2.7263,0.69992,0.35804,-0.61799,83.451 |
| 5022 | | |
| 5023 | | > fitmap #6 inMap #2 |
| 5024 | | |
| 5025 | | Fit molecule hPNP_dimer_5zf6_fit_tetramer1.pdb (#6) to map |
| 5026 | | cryosparc_P1_J138_004_volume_map_sharp.mrc (#2) using 8555 atoms |
| 5027 | | average map value = 0.111, steps = 920 |
| 5028 | | shifted from previous position = 3.95 |
| 5029 | | rotated from previous position = 10.1 degrees |
| 5030 | | atoms outside contour = 4722, contour level = 0.092417 |
| 5031 | | |
| 5032 | | Position of hPNP_dimer_5zf6_fit_tetramer1.pdb (#6) relative to |
| 5033 | | cryosparc_P1_J138_004_volume_map_sharp.mrc (#2) coordinates: |
| 5034 | | Matrix rotation and translation |
| 5035 | | -0.88994782 0.04420559 -0.45391491 374.83079362 |
| 5036 | | -0.05717949 0.97662253 0.20721711 -63.35120865 |
| 5037 | | 0.45246368 0.21036704 -0.86661544 168.59587277 |
| 5038 | | Axis 0.00345373 -0.99379614 -0.11116337 |
| 5039 | | Axis point 166.22449866 0.00000000 133.32450793 |
| 5040 | | Rotation angle (degrees) 152.86952298 |
| 5041 | | Shift along axis 45.51106552 |
| 5042 | | |
| 5043 | | |
| 5044 | | > select subtract #6 |
| 5045 | | |
| 5046 | | Nothing selected |
| 5047 | | |
| 5048 | | > hide #!2 models |
| 5049 | | |
| 5050 | | > undo |
| 5051 | | |
| 5052 | | > fitmap #6 inMap #2 |
| 5053 | | |
| 5054 | | Fit molecule hPNP_dimer_5zf6_fit_tetramer1.pdb (#6) to map |
| 5055 | | cryosparc_P1_J138_004_volume_map_sharp.mrc (#2) using 8555 atoms |
| 5056 | | average map value = 0.111, steps = 116 |
| 5057 | | shifted from previous position = 0.00709 |
| 5058 | | rotated from previous position = 0.00318 degrees |
| 5059 | | atoms outside contour = 4727, contour level = 0.092417 |
| 5060 | | |
| 5061 | | Position of hPNP_dimer_5zf6_fit_tetramer1.pdb (#6) relative to |
| 5062 | | cryosparc_P1_J138_004_volume_map_sharp.mrc (#2) coordinates: |
| 5063 | | Matrix rotation and translation |
| 5064 | | -0.88993311 0.04424511 -0.45393989 374.82642072 |
| 5065 | | -0.05716226 0.97661562 0.20725443 -63.36706439 |
| 5066 | | 0.45249479 0.21039081 -0.86659343 168.57997819 |
| 5067 | | Axis 0.00343866 -0.99379426 -0.11118072 |
| 5068 | | Axis point 166.22292888 0.00000000 133.32090242 |
| 5069 | | Rotation angle (degrees) 152.86765033 |
| 5070 | | Shift along axis 45.51987962 |
| 5071 | | |
| 5072 | | |
| 5073 | | > fitmap #6 inMap #2 |
| 5074 | | |
| 5075 | | Fit molecule hPNP_dimer_5zf6_fit_tetramer1.pdb (#6) to map |
| 5076 | | cryosparc_P1_J138_004_volume_map_sharp.mrc (#2) using 8555 atoms |
| 5077 | | average map value = 0.111, steps = 568 |
| 5078 | | shifted from previous position = 0.0114 |
| 5079 | | rotated from previous position = 0.0123 degrees |
| 5080 | | atoms outside contour = 4724, contour level = 0.092417 |
| 5081 | | |
| 5082 | | Position of hPNP_dimer_5zf6_fit_tetramer1.pdb (#6) relative to |
| 5083 | | cryosparc_P1_J138_004_volume_map_sharp.mrc (#2) coordinates: |
| 5084 | | Matrix rotation and translation |
| 5085 | | -0.88993442 0.04404381 -0.45395691 374.86893906 |
| 5086 | | -0.05737351 0.97660900 0.20722726 -63.32915866 |
| 5087 | | 0.45246548 0.21046377 -0.86659101 168.55952594 |
| 5088 | | Axis 0.00354848 -0.99379250 -0.11119295 |
| 5089 | | Axis point 166.24336123 0.00000000 133.31298230 |
| 5090 | | Rotation angle (degrees) 152.86799669 |
| 5091 | | Shift along axis 45.52362736 |
| 5092 | | |
| 5093 | | |
| 5094 | | > select add #6 |
| 5095 | | |
| 5096 | | 8555 atoms, 8618 bonds, 1174 residues, 1 model selected |
| 5097 | | |
| 5098 | | > fitmap #6 inMap #2 |
| 5099 | | |
| 5100 | | Fit molecule hPNP_dimer_5zf6_fit_tetramer1.pdb (#6) to map |
| 5101 | | cryosparc_P1_J138_004_volume_map_sharp.mrc (#2) using 8555 atoms |
| 5102 | | average map value = 0.111, steps = 180 |
| 5103 | | shifted from previous position = 0.0152 |
| 5104 | | rotated from previous position = 0.00465 degrees |
| 5105 | | atoms outside contour = 4732, contour level = 0.092417 |
| 5106 | | |
| 5107 | | Position of hPNP_dimer_5zf6_fit_tetramer1.pdb (#6) relative to |
| 5108 | | cryosparc_P1_J138_004_volume_map_sharp.mrc (#2) coordinates: |
| 5109 | | Matrix rotation and translation |
| 5110 | | -0.88993786 0.04410294 -0.45394442 374.85142718 |
| 5111 | | -0.05729523 0.97661769 0.20720798 -63.34867632 |
| 5112 | | 0.45246863 0.21041107 -0.86660217 168.58221982 |
| 5113 | | Axis 0.00351189 -0.99379482 -0.11117335 |
| 5114 | | Axis point 166.23335059 0.00000000 133.32210930 |
| 5115 | | Rotation angle (degrees) 152.86836781 |
| 5116 | | Shift along axis 45.53017211 |
| 5117 | | |
| 5118 | | |
| 5119 | | > select :605-635 |
| 5120 | | |
| 5121 | | 1296 atoms, 1317 bonds, 171 residues, 3 models selected |
| 5122 | | |
| 5123 | | > fitmap #6 inMap #2 moveWholeMolecules false |
| 5124 | | |
| 5125 | | Fit molecule hPNP_dimer_5zf6_fit_tetramer1.pdb (#6) to map |
| 5126 | | cryosparc_P1_J138_004_volume_map_sharp.mrc (#2) using 8555 atoms |
| 5127 | | average map value = 0.111, steps = 344 |
| 5128 | | shifted from previous position = 0.0176 |
| 5129 | | rotated from previous position = 0.0105 degrees |
| 5130 | | atoms outside contour = 4729, contour level = 0.092417 |
| 5131 | | |
| 5132 | | |
| 5133 | | > fitmap #6 inMap #2 moveWholeMolecules false |
| 5134 | | |
| 5135 | | Fit molecule hPNP_dimer_5zf6_fit_tetramer1.pdb (#6) to map |
| 5136 | | cryosparc_P1_J138_004_volume_map_sharp.mrc (#2) using 8555 atoms |
| 5137 | | average map value = 0.111, steps = 196 |
| 5138 | | shifted from previous position = 0.0146 |
| 5139 | | rotated from previous position = 0.00622 degrees |
| 5140 | | atoms outside contour = 4723, contour level = 0.092417 |
| 5141 | | |
| 5142 | | |
| 5143 | | > fitmap #6 inMap #2 moveWholeMolecules false |
| 5144 | | |
| 5145 | | Fit molecule hPNP_dimer_5zf6_fit_tetramer1.pdb (#6) to map |
| 5146 | | cryosparc_P1_J138_004_volume_map_sharp.mrc (#2) using 8555 atoms |
| 5147 | | average map value = 0.111, steps = 180 |
| 5148 | | shifted from previous position = 0.0248 |
| 5149 | | rotated from previous position = 0.0219 degrees |
| 5150 | | atoms outside contour = 4722, contour level = 0.092417 |
| 5151 | | |
| 5152 | | |
| 5153 | | > fitmap #6 inMap #2 moveWholeMolecules false |
| 5154 | | |
| 5155 | | Fit molecule hPNP_dimer_5zf6_fit_tetramer1.pdb (#6) to map |
| 5156 | | cryosparc_P1_J138_004_volume_map_sharp.mrc (#2) using 8555 atoms |
| 5157 | | average map value = 0.111, steps = 548 |
| 5158 | | shifted from previous position = 0.0148 |
| 5159 | | rotated from previous position = 0.00714 degrees |
| 5160 | | atoms outside contour = 4725, contour level = 0.092417 |
| 5161 | | |
| 5162 | | |
| 5163 | | > fitmap #6 inMap #2 moveWholeMolecules false |
| 5164 | | |
| 5165 | | Fit molecule hPNP_dimer_5zf6_fit_tetramer1.pdb (#6) to map |
| 5166 | | cryosparc_P1_J138_004_volume_map_sharp.mrc (#2) using 8555 atoms |
| 5167 | | average map value = 0.111, steps = 312 |
| 5168 | | shifted from previous position = 0.0297 |
| 5169 | | rotated from previous position = 0.0316 degrees |
| 5170 | | atoms outside contour = 4729, contour level = 0.092417 |
| 5171 | | |
| 5172 | | |
| 5173 | | > fitmap #6 inMap #2 moveWholeMolecules false |
| 5174 | | |
| 5175 | | Fit molecule hPNP_dimer_5zf6_fit_tetramer1.pdb (#6) to map |
| 5176 | | cryosparc_P1_J138_004_volume_map_sharp.mrc (#2) using 8555 atoms |
| 5177 | | average map value = 0.111, steps = 128 |
| 5178 | | shifted from previous position = 0.00953 |
| 5179 | | rotated from previous position = 0.0083 degrees |
| 5180 | | atoms outside contour = 4725, contour level = 0.092417 |
| 5181 | | |
| 5182 | | |
| 5183 | | > fitmap #6 inMap #2 moveWholeMolecules false |
| 5184 | | |
| 5185 | | Fit molecule hPNP_dimer_5zf6_fit_tetramer1.pdb (#6) to map |
| 5186 | | cryosparc_P1_J138_004_volume_map_sharp.mrc (#2) using 8555 atoms |
| 5187 | | average map value = 0.111, steps = 208 |
| 5188 | | shifted from previous position = 0.0222 |
| 5189 | | rotated from previous position = 0.0184 degrees |
| 5190 | | atoms outside contour = 4724, contour level = 0.092417 |
| 5191 | | |
| 5192 | | |
| 5193 | | > fitmap #6 inMap #2 moveWholeMolecules false |
| 5194 | | |
| 5195 | | Fit molecule hPNP_dimer_5zf6_fit_tetramer1.pdb (#6) to map |
| 5196 | | cryosparc_P1_J138_004_volume_map_sharp.mrc (#2) using 8555 atoms |
| 5197 | | average map value = 0.111, steps = 220 |
| 5198 | | shifted from previous position = 0.0138 |
| 5199 | | rotated from previous position = 0.00774 degrees |
| 5200 | | atoms outside contour = 4725, contour level = 0.092417 |
| 5201 | | |
| 5202 | | |
| 5203 | | > fitmap #6 inMap #2 moveWholeMolecules false |
| 5204 | | |
| 5205 | | Fit molecule hPNP_dimer_5zf6_fit_tetramer1.pdb (#6) to map |
| 5206 | | cryosparc_P1_J138_004_volume_map_sharp.mrc (#2) using 8555 atoms |
| 5207 | | average map value = 0.111, steps = 1912 |
| 5208 | | shifted from previous position = 0.0151 |
| 5209 | | rotated from previous position = 0.00815 degrees |
| 5210 | | atoms outside contour = 4724, contour level = 0.092417 |
| 5211 | | |
| 5212 | | |
| 5213 | | > fitmap #6 inMap #2 moveWholeMolecules false |
| 5214 | | |
| 5215 | | Fit molecule hPNP_dimer_5zf6_fit_tetramer1.pdb (#6) to map |
| 5216 | | cryosparc_P1_J138_004_volume_map_sharp.mrc (#2) using 8555 atoms |
| 5217 | | average map value = 0.111, steps = 388 |
| 5218 | | shifted from previous position = 0.0217 |
| 5219 | | rotated from previous position = 0.0192 degrees |
| 5220 | | atoms outside contour = 4728, contour level = 0.092417 |
| 5221 | | |
| 5222 | | |
| 5223 | | > view matrix models |
| 5224 | | > #1,0.994,-0.094744,-0.054626,207.02,0.077109,0.25294,0.9644,190.31,-0.077554,-0.96283,0.25873,213.32,#6,-0.74953,0.12709,-0.64965,417.13,-0.073566,0.95933,0.27255,-21.057,0.65787,0.25208,-0.7097,128.14,#3,0.92765,0.095145,0.36114,-49.146,-0.092392,0.99541,-0.024925,24.061,-0.36185,-0.010244,0.93218,84.812 |
| 5225 | | |
| 5226 | | > fitmap #6 inMap #2 moveWholeMolecules false |
| 5227 | | |
| 5228 | | Fit molecule hPNP_dimer_5zf6_fit_tetramer1.pdb (#6) to map |
| 5229 | | cryosparc_P1_J138_004_volume_map_sharp.mrc (#2) using 8555 atoms |
| 5230 | | average map value = 0.111, steps = 448 |
| 5231 | | shifted from previous position = 2.71 |
| 5232 | | rotated from previous position = 0.0121 degrees |
| 5233 | | atoms outside contour = 4721, contour level = 0.092417 |
| 5234 | | |
| 5235 | | |
| 5236 | | > select add #6 |
| 5237 | | |
| 5238 | | 9419 atoms, 9496 bonds, 1288 residues, 3 models selected |
| 5239 | | |
| 5240 | | > select add #3 |
| 5241 | | |
| 5242 | | 17542 atoms, 17675 bonds, 2405 residues, 3 models selected |
| 5243 | | |
| 5244 | | > select subtract #6 |
| 5245 | | |
| 5246 | | 8987 atoms, 9057 bonds, 1231 residues, 2 models selected |
| 5247 | | |
| 5248 | | > hide #!6 models |
| 5249 | | |
| 5250 | | > hide #!2 models |
| 5251 | | |
| 5252 | | > select add #1 |
| 5253 | | |
| 5254 | | 17110 atoms, 17236 bonds, 2348 residues, 2 models selected |
| 5255 | | |
| 5256 | | > select subtract #1 |
| 5257 | | |
| 5258 | | 8555 atoms, 8618 bonds, 1174 residues, 1 model selected |
| 5259 | | |
| 5260 | | > select subtract #3 |
| 5261 | | |
| 5262 | | Nothing selected |
| 5263 | | |
| 5264 | | > show #!3 models |
| 5265 | | |
| 5266 | | > show #!1 models |
| 5267 | | |
| 5268 | | > hide #!1 models |
| 5269 | | |
| 5270 | | > show #!6 models |
| 5271 | | |
| 5272 | | > select add #6 |
| 5273 | | |
| 5274 | | 8555 atoms, 8618 bonds, 1174 residues, 1 model selected |
| 5275 | | |
| 5276 | | > select add #3 |
| 5277 | | |
| 5278 | | 17110 atoms, 17236 bonds, 2348 residues, 2 models selected |
| 5279 | | |
| 5280 | | > open |
| 5281 | | > /Users/madhurikanavalli/Desktop/ImageProcessing/kasiafiles/hPNP_dimer_5zf6_fit_tetramer2.pdb |
| 5282 | | |
| 5283 | | hPNP_dimer_5zf6_fit_tetramer2.pdb title: |
| 5284 | | Crystal structure of the dimeric human pnpase [more info...] |
| 5285 | | |
| 5286 | | Chain information for hPNP_dimer_5zf6_fit_tetramer2.pdb #4 |
| 5287 | | --- |
| 5288 | | Chain | Description | UniProt |
| 5289 | | A B | polyribonucleotide nucleotidyltransferase 1, mitochondrial | PNPT1_HUMAN 46-669 |
| 5290 | | |
| 5291 | | |
| 5292 | | > hide #!6 models |
| 5293 | | |
| 5294 | | > select subtract #6 |
| 5295 | | |
| 5296 | | 8555 atoms, 8618 bonds, 1174 residues, 1 model selected |
| 5297 | | |
| 5298 | | > show #!2 models |
| 5299 | | |
| 5300 | | > select add #2 |
| 5301 | | |
| 5302 | | 8555 atoms, 8618 bonds, 1174 residues, 3 models selected |
| 5303 | | |
| 5304 | | > select add #4 |
| 5305 | | |
| 5306 | | 17110 atoms, 17236 bonds, 2348 residues, 4 models selected |
| 5307 | | |
| 5308 | | > ui tool show "Fit in Map" |
| 5309 | | |
| 5310 | | > fitmap #3 inMap #2 |
| 5311 | | |
| 5312 | | Fit molecule hPNP_dimer_5zf6_fit_tetramer1.pdb (#3) to map |
| 5313 | | cryosparc_P1_J138_004_volume_map_sharp.mrc (#2) using 8555 atoms |
| 5314 | | average map value = 0.1262, steps = 2000 |
| 5315 | | shifted from previous position = 2.56 |
| 5316 | | rotated from previous position = 0.165 degrees |
| 5317 | | atoms outside contour = 4321, contour level = 0.092417 |
| 5318 | | |
| 5319 | | Position of hPNP_dimer_5zf6_fit_tetramer1.pdb (#3) relative to |
| 5320 | | cryosparc_P1_J138_004_volume_map_sharp.mrc (#2) coordinates: |
| 5321 | | Matrix rotation and translation |
| 5322 | | 0.99008565 0.08020231 0.11531698 -66.56585309 |
| 5323 | | -0.08547902 0.99547290 0.04155783 -35.70187256 |
| 5324 | | -0.11146190 -0.05100299 0.99245904 6.18707679 |
| 5325 | | Axis -0.31300845 0.76688716 -0.56027654 |
| 5326 | | Axis point -35.49371356 -0.00000000 601.36159142 |
| 5327 | | Rotation angle (degrees) 8.50274218 |
| 5328 | | Shift along axis -10.01010685 |
| 5329 | | |
| 5330 | | |
| 5331 | | > fitmap #3 inMap #2 |
| 5332 | | |
| 5333 | | Fit molecule hPNP_dimer_5zf6_fit_tetramer1.pdb (#3) to map |
| 5334 | | cryosparc_P1_J138_004_volume_map_sharp.mrc (#2) using 8555 atoms |
| 5335 | | average map value = 0.1265, steps = 2000 |
| 5336 | | shifted from previous position = 0.239 |
| 5337 | | rotated from previous position = 0.818 degrees |
| 5338 | | atoms outside contour = 4303, contour level = 0.092417 |
| 5339 | | |
| 5340 | | Position of hPNP_dimer_5zf6_fit_tetramer1.pdb (#3) relative to |
| 5341 | | cryosparc_P1_J138_004_volume_map_sharp.mrc (#2) coordinates: |
| 5342 | | Matrix rotation and translation |
| 5343 | | 0.99012491 0.07955173 0.11543040 -66.35338000 |
| 5344 | | -0.08323899 0.99615079 0.02747518 -33.39555135 |
| 5345 | | -0.11280039 -0.03681217 0.99293552 3.37679073 |
| 5346 | | Axis -0.22351799 0.79352600 -0.56600018 |
| 5347 | | Axis point -75.44975106 0.00000000 594.67655370 |
| 5348 | | Rotation angle (degrees) 8.26825993 |
| 5349 | | Shift along axis -13.58032821 |
| 5350 | | |
| 5351 | | |
| 5352 | | > fitmap #4 inMap #2 |
| 5353 | | |
| 5354 | | Fit molecule hPNP_dimer_5zf6_fit_tetramer2.pdb (#4) to map |
| 5355 | | cryosparc_P1_J138_004_volume_map_sharp.mrc (#2) using 8555 atoms |
| 5356 | | average map value = 0.1106, steps = 296 |
| 5357 | | shifted from previous position = 11.4 |
| 5358 | | rotated from previous position = 15 degrees |
| 5359 | | atoms outside contour = 4757, contour level = 0.092417 |
| 5360 | | |
| 5361 | | Position of hPNP_dimer_5zf6_fit_tetramer2.pdb (#4) relative to |
| 5362 | | cryosparc_P1_J138_004_volume_map_sharp.mrc (#2) coordinates: |
| 5363 | | Matrix rotation and translation |
| 5364 | | 0.99975115 0.02225007 0.00160437 -42.37113273 |
| 5365 | | -0.02229050 0.99922195 0.03253675 -39.86667288 |
| 5366 | | -0.00087917 -0.03256442 0.99946925 -26.34167349 |
| 5367 | | Axis -0.82491227 0.03146955 -0.56438411 |
| 5368 | | Axis point 0.00000000 12.03996179 1272.54105826 |
| 5369 | | Rotation angle (degrees) 2.26144694 |
| 5370 | | Shift along axis 48.56470264 |
| 5371 | | |
| 5372 | | |
| 5373 | | > select subtract #4 |
| 5374 | | |
| 5375 | | 8555 atoms, 8618 bonds, 1174 residues, 3 models selected |
| 5376 | | |
| 5377 | | > select subtract #3 |
| 5378 | | |
| 5379 | | 2 models selected |
| 5380 | | |
| 5381 | | > select subtract #2 |
| 5382 | | |
| 5383 | | Nothing selected |
| 5384 | | |
| 5385 | | > ui mousemode right "rotate selected models" |
| 5386 | | |
| 5387 | | > select add #3 |
| 5388 | | |
| 5389 | | 8555 atoms, 8618 bonds, 1174 residues, 1 model selected |
| 5390 | | |
| 5391 | | > view matrix models |
| 5392 | | > #3,0.88251,0.23211,0.40903,-75.275,-0.21961,0.97246,-0.078009,62.282,-0.41588,-0.020986,0.90918,100.45 |
| 5393 | | |
| 5394 | | > ui mousemode right "translate selected models" |
| 5395 | | |
| 5396 | | > view matrix models |
| 5397 | | > #3,0.88251,0.23211,0.40903,-75.557,-0.21961,0.97246,-0.078009,61.908,-0.41588,-0.020986,0.90918,99.178 |
| 5398 | | |
| 5399 | | > fitmap #3 inMap #2 |
| 5400 | | |
| 5401 | | Fit molecule hPNP_dimer_5zf6_fit_tetramer1.pdb (#3) to map |
| 5402 | | cryosparc_P1_J138_004_volume_map_sharp.mrc (#2) using 8555 atoms |
| 5403 | | average map value = 0.127, steps = 272 |
| 5404 | | shifted from previous position = 1.55 |
| 5405 | | rotated from previous position = 8.41 degrees |
| 5406 | | atoms outside contour = 4288, contour level = 0.092417 |
| 5407 | | |
| 5408 | | Position of hPNP_dimer_5zf6_fit_tetramer1.pdb (#3) relative to |
| 5409 | | cryosparc_P1_J138_004_volume_map_sharp.mrc (#2) coordinates: |
| 5410 | | Matrix rotation and translation |
| 5411 | | 0.98988621 0.08222609 0.11560346 -66.94222621 |
| 5412 | | -0.08667313 0.99565733 0.03397417 -34.07262077 |
| 5413 | | -0.11230787 -0.04365027 0.99271426 4.88957146 |
| 5414 | | Axis -0.26393700 0.77493934 -0.57428763 |
| 5415 | | Axis point -54.45818067 0.00000000 600.65902030 |
| 5416 | | Rotation angle (degrees) 8.45607029 |
| 5417 | | Shift along axis -11.54370437 |
| 5418 | | |
| 5419 | | |
| 5420 | | > select subtract #3 |
| 5421 | | |
| 5422 | | Nothing selected |
| 5423 | | |
| 5424 | | > select add #4 |
| 5425 | | |
| 5426 | | 8555 atoms, 8618 bonds, 1174 residues, 1 model selected |
| 5427 | | |
| 5428 | | > view matrix models |
| 5429 | | > #4,0.96636,0.045793,0.25307,-32.02,-0.038117,0.99865,-0.035156,22.745,-0.25434,0.024327,0.96681,48.2 |
| 5430 | | |
| 5431 | | > ui mousemode right "rotate selected models" |
| 5432 | | |
| 5433 | | > view matrix models |
| 5434 | | > #4,0.98649,-0.13285,0.095881,19.07,0.13318,0.99109,0.0030574,-13.022,-0.095433,0.009753,0.99539,17.369 |
| 5435 | | |
| 5436 | | > fitmap #3 inMap #2 moveWholeMolecules false |
| 5437 | | |
| 5438 | | Fit molecule hPNP_dimer_5zf6_fit_tetramer1.pdb (#3) to map |
| 5439 | | cryosparc_P1_J138_004_volume_map_sharp.mrc (#2) using 8555 atoms |
| 5440 | | average map value = 0.1267, steps = 2000 |
| 5441 | | shifted from previous position = 0.231 |
| 5442 | | rotated from previous position = 0.209 degrees |
| 5443 | | atoms outside contour = 4265, contour level = 0.092417 |
| 5444 | | |
| 5445 | | |
| 5446 | | > fitmap #3 inMap #2 moveWholeMolecules false |
| 5447 | | |
| 5448 | | Fit molecule hPNP_dimer_5zf6_fit_tetramer1.pdb (#3) to map |
| 5449 | | cryosparc_P1_J138_004_volume_map_sharp.mrc (#2) using 8555 atoms |
| 5450 | | average map value = 0.1257, steps = 2000 |
| 5451 | | shifted from previous position = 0.153 |
| 5452 | | rotated from previous position = 0.802 degrees |
| 5453 | | atoms outside contour = 4316, contour level = 0.092417 |
| 5454 | | |
| 5455 | | |
| 5456 | | > select subtract #4 |
| 5457 | | |
| 5458 | | Nothing selected |
| 5459 | | |
| 5460 | | > select add #4 |
| 5461 | | |
| 5462 | | 8555 atoms, 8618 bonds, 1174 residues, 1 model selected |
| 5463 | | |
| 5464 | | > view matrix models |
| 5465 | | > #4,0.98865,0.077128,0.12895,-24.965,-0.061721,0.99092,-0.11949,40.165,-0.13699,0.11017,0.98443,7.9262 |
| 5466 | | |
| 5467 | | > fitmap #3 inMap #2 moveWholeMolecules false |
| 5468 | | |
| 5469 | | Fit molecule hPNP_dimer_5zf6_fit_tetramer1.pdb (#3) to map |
| 5470 | | cryosparc_P1_J138_004_volume_map_sharp.mrc (#2) using 8555 atoms |
| 5471 | | average map value = 0.1256, steps = 2000 |
| 5472 | | shifted from previous position = 0.447 |
| 5473 | | rotated from previous position = 0.959 degrees |
| 5474 | | atoms outside contour = 4316, contour level = 0.092417 |
| 5475 | | |
| 5476 | | |
| 5477 | | > fitmap #3 inMap #2 moveWholeMolecules false |
| 5478 | | |
| 5479 | | Fit molecule hPNP_dimer_5zf6_fit_tetramer1.pdb (#3) to map |
| 5480 | | cryosparc_P1_J138_004_volume_map_sharp.mrc (#2) using 8555 atoms |
| 5481 | | average map value = 0.1267, steps = 2000 |
| 5482 | | shifted from previous position = 0.369 |
| 5483 | | rotated from previous position = 0.333 degrees |
| 5484 | | atoms outside contour = 4261, contour level = 0.092417 |
| 5485 | | |
| 5486 | | |
| 5487 | | > fitmap #4 inMap #2 |
| 5488 | | |
| 5489 | | Fit molecule hPNP_dimer_5zf6_fit_tetramer2.pdb (#4) to map |
| 5490 | | cryosparc_P1_J138_004_volume_map_sharp.mrc (#2) using 8555 atoms |
| 5491 | | average map value = 0.1106, steps = 464 |
| 5492 | | shifted from previous position = 2.2 |
| 5493 | | rotated from previous position = 8.71 degrees |
| 5494 | | atoms outside contour = 4757, contour level = 0.092417 |
| 5495 | | |
| 5496 | | Position of hPNP_dimer_5zf6_fit_tetramer2.pdb (#4) relative to |
| 5497 | | cryosparc_P1_J138_004_volume_map_sharp.mrc (#2) coordinates: |
| 5498 | | Matrix rotation and translation |
| 5499 | | 0.99975184 0.02221316 0.00168369 -42.37411242 |
| 5500 | | -0.02225622 0.99922206 0.03255658 -39.87679425 |
| 5501 | | -0.00095919 -0.03258598 0.99946848 -26.32053100 |
| 5502 | | Axis -0.82544529 0.03348892 -0.56348786 |
| 5503 | | Axis point 0.00000000 9.69649265 1274.93904155 |
| 5504 | | Rotation angle (degrees) 2.26142362 |
| 5505 | | Shift along axis 48.47338052 |
| 5506 | | |
| 5507 | | |
| 5508 | | > select subtract #4 |
| 5509 | | |
| 5510 | | Nothing selected |
| 5511 | | |
| 5512 | | > ui tool show "Fit to Segments" |
| 5513 | | |
| 5514 | | > select #1/A-B:293 |
| 5515 | | |
| 5516 | | 18 atoms, 16 bonds, 2 residues, 1 model selected |
| 5517 | | |
| 5518 | | > select #1/A-B:293-367 |
| 5519 | | |
| 5520 | | 1256 atoms, 1276 bonds, 150 residues, 1 model selected |
| 5521 | | |
| 5522 | | > select add #4 |
| 5523 | | |
| 5524 | | 9811 atoms, 9894 bonds, 1324 residues, 2 models selected |
| 5525 | | |
| 5526 | | > select subtract #4 |
| 5527 | | |
| 5528 | | 1256 atoms, 1276 bonds, 150 residues, 1 model selected |
| 5529 | | |
| 5530 | | > select :605-635 |
| 5531 | | |
| 5532 | | 1728 atoms, 1756 bonds, 228 residues, 4 models selected |
| 5533 | | |
| 5534 | | > fitmap #4 inMap #2 moveWholeMolecules false |
| 5535 | | |
| 5536 | | Fit molecule hPNP_dimer_5zf6_fit_tetramer2.pdb (#4) to map |
| 5537 | | cryosparc_P1_J138_004_volume_map_sharp.mrc (#2) using 8555 atoms |
| 5538 | | average map value = 0.1106, steps = 776 |
| 5539 | | shifted from previous position = 0.00595 |
| 5540 | | rotated from previous position = 0.00471 degrees |
| 5541 | | atoms outside contour = 4758, contour level = 0.092417 |
| 5542 | | |
| 5543 | | |
| 5544 | | > fitmap #4 inMap #2 moveWholeMolecules false |
| 5545 | | |
| 5546 | | Fit molecule hPNP_dimer_5zf6_fit_tetramer2.pdb (#4) to map |
| 5547 | | cryosparc_P1_J138_004_volume_map_sharp.mrc (#2) using 8555 atoms |
| 5548 | | average map value = 0.1106, steps = 384 |
| 5549 | | shifted from previous position = 0.0135 |
| 5550 | | rotated from previous position = 0.0157 degrees |
| 5551 | | atoms outside contour = 4757, contour level = 0.092417 |
| 5552 | | |
| 5553 | | |
| 5554 | | > cd /Users/madhurikanavalli/Desktop/test |
| 5555 | | |
| 5556 | | Current working directory is: /Users/madhurikanavalli/Desktop/test |
| 5557 | | |
| 5558 | | > save /Users/madhurikanavalli/Desktop/test/dimerfit_Kasia.cxs |
| 5559 | | |
| 5560 | | ——— End of log from Fri Aug 30 15:18:41 2024 ——— |
| 5561 | | |
| 5562 | | > view name session-start |
| 5563 | | |
| 5564 | | opened ChimeraX session |
| 5565 | | |
| 5566 | | > open /Users/madhurikanavalli/Downloads/cryosparc_P1_J482_004_volume_map.mrc |
| 5567 | | |
| 5568 | | Opened cryosparc_P1_J482_004_volume_map.mrc as #2, grid size 352,352,352, |
| 5569 | | pixel 0.846, shown at level 0.0477, step 2, values float32 |
| 5570 | | |
| 5571 | | > volume #2 level 0.1132 |
| 5572 | | |
| 5573 | | > set bgColor white |
| 5574 | | |
| 5575 | | > volume #2 level 0.1025 |
| 5576 | | |
| 5577 | | > volume #2 level 0.1001 |
| 5578 | | |
| 5579 | | > volume #2 level 0.08461 |
| 5580 | | |
| 5581 | | > select add #4 |
| 5582 | | |
| 5583 | | 9851 atoms, 9935 bonds, 1345 residues, 4 models selected |
| 5584 | | |
| 5585 | | > select subtract #4 |
| 5586 | | |
| 5587 | | 1296 atoms, 1317 bonds, 171 residues, 3 models selected |
| 5588 | | |
| 5589 | | > select add #3 |
| 5590 | | |
| 5591 | | 9419 atoms, 9496 bonds, 1288 residues, 3 models selected |
| 5592 | | |
| 5593 | | > select subtract #3 |
| 5594 | | |
| 5595 | | 864 atoms, 878 bonds, 114 residues, 2 models selected |
| 5596 | | |
| 5597 | | > select add #3 |
| 5598 | | |
| 5599 | | 9419 atoms, 9496 bonds, 1288 residues, 3 models selected |
| 5600 | | |
| 5601 | | > select add #6 |
| 5602 | | |
| 5603 | | 17542 atoms, 17675 bonds, 2405 residues, 3 models selected |
| 5604 | | |
| 5605 | | > view matrix models |
| 5606 | | > #1,0.994,-0.094744,-0.054626,190.68,0.077109,0.25294,0.9644,136.09,-0.077554,-0.96283,0.25873,179.49,#6,-0.74953,0.12709,-0.64965,400.79,-0.073566,0.95933,0.27255,-75.279,0.65787,0.25208,-0.7097,94.31,#3,0.92681,0.10093,0.36172,-64.082,-0.094651,0.99489,-0.035082,-27.656,-0.36341,-0.0017227,0.93163,49.036 |
| 5607 | | |
| 5608 | | > view matrix models |
| 5609 | | > #1,0.994,-0.094744,-0.054626,189.74,0.077109,0.25294,0.9644,121.52,-0.077554,-0.96283,0.25873,187.27,#6,-0.74953,0.12709,-0.64965,399.84,-0.073566,0.95933,0.27255,-89.852,0.65787,0.25208,-0.7097,102.09,#3,0.92681,0.10093,0.36172,-65.027,-0.094651,0.99489,-0.035082,-42.229,-0.36341,-0.0017227,0.93163,56.814 |
| 5610 | | |
| 5611 | | > view matrix models |
| 5612 | | > #1,0.994,-0.094744,-0.054626,191.44,0.077109,0.25294,0.9644,104.17,-0.077554,-0.96283,0.25873,210.37,#6,-0.74953,0.12709,-0.64965,401.54,-0.073566,0.95933,0.27255,-107.2,0.65787,0.25208,-0.7097,125.19,#3,0.92681,0.10093,0.36172,-63.324,-0.094651,0.99489,-0.035082,-59.577,-0.36341,-0.0017227,0.93163,79.914 |
| 5613 | | |
| 5614 | | > view matrix models |
| 5615 | | > #1,0.994,-0.094744,-0.054626,189.26,0.077109,0.25294,0.9644,133.92,-0.077554,-0.96283,0.25873,174.38,#6,-0.74953,0.12709,-0.64965,399.36,-0.073566,0.95933,0.27255,-77.452,0.65787,0.25208,-0.7097,89.203,#3,0.92681,0.10093,0.36172,-65.504,-0.094651,0.99489,-0.035082,-29.83,-0.36341,-0.0017227,0.93163,43.928 |
| 5616 | | |
| 5617 | | > view matrix models |
| 5618 | | > #1,0.994,-0.094744,-0.054626,160.99,0.077109,0.25294,0.9644,160.74,-0.077554,-0.96283,0.25873,187.51,#6,-0.74953,0.12709,-0.64965,371.09,-0.073566,0.95933,0.27255,-50.634,0.65787,0.25208,-0.7097,102.33,#3,0.92681,0.10093,0.36172,-93.774,-0.094651,0.99489,-0.035082,-3.0112,-0.36341,-0.0017227,0.93163,57.056 |
| 5619 | | |
| 5620 | | > view matrix models |
| 5621 | | > #1,0.994,-0.094744,-0.054626,162.53,0.077109,0.25294,0.9644,159.38,-0.077554,-0.96283,0.25873,186.26,#6,-0.74953,0.12709,-0.64965,372.63,-0.073566,0.95933,0.27255,-51.991,0.65787,0.25208,-0.7097,101.08,#3,0.92681,0.10093,0.36172,-92.239,-0.094651,0.99489,-0.035082,-4.368,-0.36341,-0.0017227,0.93163,55.803 |
| 5622 | | |
| 5623 | | > ui mousemode right "rotate selected models" |
| 5624 | | |
| 5625 | | > view matrix models |
| 5626 | | > #1,0.92939,-0.058623,-0.36441,164.52,0.36859,0.096106,0.92461,157.81,-0.019182,-0.99364,0.11093,186.66,#6,-0.76215,-0.18968,-0.61899,435.59,-0.22171,0.97477,-0.025711,12.579,0.60825,0.11764,-0.78498,147.92,#3,0.94763,-0.1971,0.25131,-14.75,0.152,0.97036,0.18786,-90.399,-0.28088,-0.13982,0.9495,64.215 |
| 5627 | | |
| 5628 | | > view matrix models |
| 5629 | | > #1,0.96327,-0.086547,0.2542,161.13,-0.18677,0.46424,0.86579,161.41,-0.19294,-0.88147,0.43103,186.05,#6,-0.68636,0.42515,-0.59005,291.88,0.0013985,0.8121,0.58352,-77.28,0.72726,0.39968,-0.55798,37.696,#3,0.82694,0.39195,0.40316,-139.78,-0.26967,0.90562,-0.32731,105.56,-0.4934,0.16195,0.85459,63.291 |
| 5630 | | |
| 5631 | | Desktop color scheme is dark |
| 5632 | | |
| 5633 | | > lighting full |
| 5634 | | |
| 5635 | | > view matrix models |
| 5636 | | > #1,0.92599,0.2946,-0.23612,163.44,0.14324,0.30452,0.94167,158.98,0.34932,-0.9058,0.23979,183.4,#6,-0.94666,-0.068404,-0.31488,406.44,-0.15974,0.9483,0.27424,-33.373,0.27984,0.30991,-0.90865,188.22,#3,0.99828,0.01072,-0.057691,0.50116,-0.0147,0.99751,-0.069014,-12.047,0.056808,0.069743,0.99595,-46.681 |
| 5637 | | |
| 5638 | | > ui mousemode right "translate selected models" |
| 5639 | | |
| 5640 | | > view matrix models |
| 5641 | | > #1,0.92599,0.2946,-0.23612,161.4,0.14324,0.30452,0.94167,143.18,0.34932,-0.9058,0.23979,186.35,#6,-0.94666,-0.068404,-0.31488,404.41,-0.15974,0.9483,0.27424,-49.176,0.27984,0.30991,-0.90865,191.17,#3,0.99828,0.01072,-0.057691,-1.5331,-0.0147,0.99751,-0.069014,-27.85,0.056808,0.069743,0.99595,-43.732 |
| 5642 | | |
| 5643 | | > show #!6 models |
| 5644 | | |
| 5645 | | > hide #!6 models |
| 5646 | | |
| 5647 | | > select add #4 |
| 5648 | | |
| 5649 | | 26097 atoms, 26293 bonds, 3579 residues, 4 models selected |
| 5650 | | |
| 5651 | | > select subtract #3 |
| 5652 | | |
| 5653 | | 17542 atoms, 17675 bonds, 2405 residues, 3 models selected |
| 5654 | | |
| 5655 | | > view matrix models |
| 5656 | | > #1,0.92599,0.2946,-0.23612,159.75,0.14324,0.30452,0.94167,90.273,0.34932,-0.9058,0.23979,167.09,#6,-0.94666,-0.068404,-0.31488,402.75,-0.15974,0.9483,0.27424,-102.08,0.27984,0.30991,-0.90865,171.91,#4,0.96635,0.045752,0.25314,-33.638,-0.038077,0.99866,-0.035137,-30.414,-0.25441,0.024315,0.96679,26.911 |
| 5657 | | |
| 5658 | | > ui mousemode right "rotate selected models" |
| 5659 | | |
| 5660 | | > view matrix models |
| 5661 | | > #1,0.61907,0.58221,-0.52704,153.93,0.26992,0.47248,0.83899,79.331,0.73749,-0.66166,0.13535,146.15,#6,-0.9085,-0.41347,0.060664,407.35,-0.37329,0.86818,0.32698,-61.974,-0.18786,0.27441,-0.94308,255.17,#4,0.96004,-0.20089,-0.19484,74.965,0.18128,0.97681,-0.11393,-56.416,0.2132,0.074054,0.9742,-70.527 |
| 5662 | | |
| 5663 | | > view matrix models |
| 5664 | | > #1,0.6268,0.5183,-0.58179,160.01,0.301,0.52764,0.79435,76.265,0.71869,-0.67302,0.17472,146.07,#6,-0.88524,-0.46514,-0.002032,427.41,-0.43651,0.82923,0.34904,-47.779,-0.16067,0.30987,-0.9371,241.9,#4,0.95184,-0.26949,-0.14617,82.582,0.24844,0.95738,-0.14729,-60.738,0.17964,0.10388,0.97823,-70.376 |
| 5665 | | |
| 5666 | | > ui mousemode right "translate selected models" |
| 5667 | | |
| 5668 | | > view matrix models |
| 5669 | | > #1,0.6268,0.5183,-0.58179,139.9,0.301,0.52764,0.79435,59.265,0.71869,-0.67302,0.17472,146.39,#6,-0.88524,-0.46514,-0.002032,407.3,-0.43651,0.82923,0.34904,-64.778,-0.16067,0.30987,-0.9371,242.23,#4,0.95184,-0.26949,-0.14617,62.471,0.24844,0.95738,-0.14729,-77.738,0.17964,0.10388,0.97823,-70.05 |
| 5670 | | |
| 5671 | | > ui mousemode right "rotate selected models" |
| 5672 | | |
| 5673 | | > view matrix models |
| 5674 | | > #1,0.56833,0.70828,-0.41874,122.46,0.33149,0.26869,0.90439,73.602,0.75307,-0.6528,-0.082087,152.32,#6,-0.92587,-0.31765,0.2046,341.15,-0.29507,0.9461,0.13355,-72.727,-0.23599,0.063284,-0.96969,316.14,#4,0.9477,-0.072153,-0.31091,49.129,0.10669,0.98969,0.09553,-90.672,0.30081,-0.1237,0.94563,-45.794 |
| 5675 | | |
| 5676 | | > view matrix models |
| 5677 | | > #1,0.54644,0.67075,-0.50149,128.07,0.28728,0.41234,0.86455,65.478,0.78668,-0.61649,0.032625,145.6,#6,-0.89812,-0.40253,0.17703,361.73,-0.34868,0.89718,0.27108,-78.956,-0.26794,0.18173,-0.94614,289.19,#4,0.93818,-0.1649,-0.30435,67.269,0.15659,0.98631,-0.051703,-79.126,0.30871,0.00084819,0.95115,-71.212 |
| 5678 | | |
| 5679 | | > ui mousemode right "translate selected models" |
| 5680 | | |
| 5681 | | > view matrix models |
| 5682 | | > #1,0.54644,0.67075,-0.50149,108.49,0.28728,0.41234,0.86455,68.46,0.78668,-0.61649,0.032625,139.36,#6,-0.89812,-0.40253,0.17703,342.14,-0.34868,0.89718,0.27108,-75.973,-0.26794,0.18173,-0.94614,282.95,#4,0.93818,-0.1649,-0.30435,47.685,0.15659,0.98631,-0.051703,-76.143,0.30871,0.00084819,0.95115,-77.451 |
| 5683 | | |
| 5684 | | > view matrix models |
| 5685 | | > #1,0.54644,0.67075,-0.50149,111.2,0.28728,0.41234,0.86455,87.267,0.78668,-0.61649,0.032625,138.22,#6,-0.89812,-0.40253,0.17703,344.86,-0.34868,0.89718,0.27108,-57.167,-0.26794,0.18173,-0.94614,281.81,#4,0.93818,-0.1649,-0.30435,50.401,0.15659,0.98631,-0.051703,-57.337,0.30871,0.00084819,0.95115,-78.588 |
| 5686 | | |
| 5687 | | > select subtract #4 |
| 5688 | | |
| 5689 | | 8987 atoms, 9057 bonds, 1231 residues, 2 models selected |
| 5690 | | |
| 5691 | | > select add #3 |
| 5692 | | |
| 5693 | | 17542 atoms, 17675 bonds, 2405 residues, 3 models selected |
| 5694 | | |
| 5695 | | > view matrix models |
| 5696 | | > #1,0.54644,0.67075,-0.50149,93.636,0.28728,0.41234,0.86455,86.962,0.78668,-0.61649,0.032625,135.05,#6,-0.89812,-0.40253,0.17703,327.29,-0.34868,0.89718,0.27108,-57.471,-0.26794,0.18173,-0.94614,278.64,#3,0.99828,0.01072,-0.057691,-19.1,-0.0147,0.99751,-0.069014,-28.154,0.056808,0.069743,0.99595,-46.902 |
| 5697 | | |
| 5698 | | > transparency 50 |
| 5699 | | |
| 5700 | | > ui tool show "Fit in Map" |
| 5701 | | |
| 5702 | | > fitmap #3 inMap #2 |
| 5703 | | |
| 5704 | | Fit molecule hPNP_dimer_5zf6_fit_tetramer1.pdb (#3) to map |
| 5705 | | cryosparc_P1_J482_004_volume_map.mrc (#2) using 8555 atoms |
| 5706 | | average map value = 0.1145, steps = 1280 |
| 5707 | | shifted from previous position = 3.75 |
| 5708 | | rotated from previous position = 12.3 degrees |
| 5709 | | atoms outside contour = 4232, contour level = 0.084608 |
| 5710 | | |
| 5711 | | Position of hPNP_dimer_5zf6_fit_tetramer1.pdb (#3) relative to |
| 5712 | | cryosparc_P1_J482_004_volume_map.mrc (#2) coordinates: |
| 5713 | | Matrix rotation and translation |
| 5714 | | 0.99192569 0.06320433 0.10994826 -62.72498723 |
| 5715 | | -0.06914256 0.99629928 0.05105907 -41.19333953 |
| 5716 | | -0.10631421 -0.05824891 0.99262497 10.91865642 |
| 5717 | | Axis -0.39589431 0.78326468 -0.47933718 |
| 5718 | | Axis point 2.83869525 0.00000000 616.31281519 |
| 5719 | | Rotation angle (degrees) 7.93513801 |
| 5720 | | Shift along axis -12.66654047 |
| 5721 | | |
| 5722 | | |
| 5723 | | > view matrix models |
| 5724 | | > #1,0.54644,0.67075,-0.50149,93.206,0.28728,0.41234,0.86455,84.978,0.78668,-0.61649,0.032625,131.42,#6,-0.89812,-0.40253,0.17703,326.86,-0.34868,0.89718,0.27108,-59.456,-0.26794,0.18173,-0.94614,275.01,#3,0.99193,0.063204,0.10995,-63.155,-0.069143,0.9963,0.051059,-43.178,-0.10631,-0.058249,0.99262,7.289 |
| 5725 | | |
| 5726 | | > view matrix models |
| 5727 | | > #1,0.54644,0.67075,-0.50149,92.22,0.28728,0.41234,0.86455,84.636,0.78668,-0.61649,0.032625,131.84,#6,-0.89812,-0.40253,0.17703,325.88,-0.34868,0.89718,0.27108,-59.798,-0.26794,0.18173,-0.94614,275.43,#3,0.99193,0.063204,0.10995,-64.141,-0.069143,0.9963,0.051059,-43.52,-0.10631,-0.058249,0.99262,7.7078 |
| 5728 | | |
| 5729 | | > select subtract #3 |
| 5730 | | |
| 5731 | | 8987 atoms, 9057 bonds, 1231 residues, 2 models selected |
| 5732 | | |
| 5733 | | > view matrix models |
| 5734 | | > #1,0.54644,0.67075,-0.50149,94.529,0.28728,0.41234,0.86455,85.746,0.78668,-0.61649,0.032625,118.28,#6,-0.89812,-0.40253,0.17703,328.18,-0.34868,0.89718,0.27108,-58.687,-0.26794,0.18173,-0.94614,261.87 |
| 5735 | | |
| 5736 | | > view matrix models |
| 5737 | | > #1,0.54644,0.67075,-0.50149,96.546,0.28728,0.41234,0.86455,85.767,0.78668,-0.61649,0.032625,105.23,#6,-0.89812,-0.40253,0.17703,330.2,-0.34868,0.89718,0.27108,-58.666,-0.26794,0.18173,-0.94614,248.82 |
| 5738 | | |
| 5739 | | > ui mousemode right "rotate selected models" |
| 5740 | | |
| 5741 | | > view matrix models |
| 5742 | | > #1,0.54644,0.67075,-0.50149,96.546,0.28728,0.41234,0.86455,85.767,0.78668,-0.61649,0.032625,105.23,#6,-0.89812,-0.40253,0.17703,330.2,-0.34868,0.89718,0.27108,-58.666,-0.26794,0.18173,-0.94614,248.82 |
| 5743 | | |
| 5744 | | > ui mousemode right "rotate selected models" |
| 5745 | | |
| 5746 | | > select add #3 |
| 5747 | | |
| 5748 | | 17542 atoms, 17675 bonds, 2405 residues, 3 models selected |
| 5749 | | |
| 5750 | | > view matrix models |
| 5751 | | > #1,0.42372,0.78352,-0.45449,107.25,0.26163,0.3745,0.88955,88.23,0.86718,-0.49583,-0.04631,97.495,#6,-0.85867,-0.37985,0.3441,306.29,-0.30263,0.91759,0.25776,-67.573,-0.41366,0.1172,-0.90286,276.9,#3,0.99096,0.12353,-0.052365,-41.689,-0.12099,0.99143,0.049217,-32.923,0.057996,-0.042437,0.99741,-25.732 |
| 5752 | | |
| 5753 | | > view matrix models |
| 5754 | | > #1,0.48037,0.80395,-0.35057,97.566,0.14733,0.32006,0.93587,99.935,0.8646,-0.50122,0.035301,95.294,#6,-0.90139,-0.26766,0.34038,283.39,-0.17286,0.94315,0.28389,-90.096,-0.39701,0.19706,-0.89641,254.77,#3,0.97189,0.23512,-0.012238,-68.434,-0.23532,0.97172,-0.019439,5.7376,0.0073212,0.021773,0.99974,-29.674 |
| 5755 | | |
| 5756 | | > fitmap #3 inMap #2 |
| 5757 | | |
| 5758 | | Fit molecule hPNP_dimer_5zf6_fit_tetramer1.pdb (#3) to map |
| 5759 | | cryosparc_P1_J482_004_volume_map.mrc (#2) using 8555 atoms |
| 5760 | | average map value = 0.1145, steps = 1020 |
| 5761 | | shifted from previous position = 4.13 |
| 5762 | | rotated from previous position = 12.7 degrees |
| 5763 | | atoms outside contour = 4240, contour level = 0.084608 |
| 5764 | | |
| 5765 | | Position of hPNP_dimer_5zf6_fit_tetramer1.pdb (#3) relative to |
| 5766 | | cryosparc_P1_J482_004_volume_map.mrc (#2) coordinates: |
| 5767 | | Matrix rotation and translation |
| 5768 | | 0.99191155 0.06335532 0.10998890 -62.76825757 |
| 5769 | | -0.06928609 0.99629434 0.05096082 -41.15660376 |
| 5770 | | -0.10635268 -0.05816933 0.99262552 10.90286608 |
| 5771 | | Axis -0.39506002 0.78317416 -0.48017268 |
| 5772 | | Axis point 2.56900071 0.00000000 616.37826305 |
| 5773 | | Rotation angle (degrees) 7.93898321 |
| 5774 | | Shift along axis -12.67081790 |
| 5775 | | |
| 5776 | | |
| 5777 | | > fitmap #4 inMap #2 |
| 5778 | | |
| 5779 | | Fit molecule hPNP_dimer_5zf6_fit_tetramer2.pdb (#4) to map |
| 5780 | | cryosparc_P1_J482_004_volume_map.mrc (#2) using 8555 atoms |
| 5781 | | average map value = 0.1047, steps = 804 |
| 5782 | | shifted from previous position = 5.34 |
| 5783 | | rotated from previous position = 20.5 degrees |
| 5784 | | atoms outside contour = 4422, contour level = 0.084608 |
| 5785 | | |
| 5786 | | Position of hPNP_dimer_5zf6_fit_tetramer2.pdb (#4) relative to |
| 5787 | | cryosparc_P1_J482_004_volume_map.mrc (#2) coordinates: |
| 5788 | | Matrix rotation and translation |
| 5789 | | 0.99999092 0.00412006 0.00108643 -39.00797240 |
| 5790 | | -0.00412304 0.99998770 0.00275780 -38.99266428 |
| 5791 | | -0.00107505 -0.00276225 0.99999561 -27.76974191 |
| 5792 | | Axis -0.54366522 0.21288185 -0.81185556 |
| 5793 | | Axis point -11301.91372520 4764.89551107 0.00000000 |
| 5794 | | Rotation angle (degrees) 0.29087494 |
| 5795 | | Shift along axis 35.45146656 |
| 5796 | | |
| 5797 | | |
| 5798 | | > view matrix models |
| 5799 | | > #1,0.50877,0.851,-0.13021,86.529,-0.0070294,0.15535,0.98783,117.65,0.86087,-0.50167,0.08502,94.082,#6,-0.92059,-0.046384,0.38776,226.11,0.053879,0.96834,0.24375,-116.88,-0.38679,0.24528,-0.88895,241.01,#3,0.94978,0.28585,0.12733,-102.22,-0.28518,0.95818,-0.023838,20.523,-0.12882,-0.013672,0.99157,6.4689 |
| 5800 | | |
| 5801 | | > view matrix models |
| 5802 | | > #1,0.41106,0.90215,0.13096,88.402,-0.17226,-0.0642,0.98296,139.1,0.89519,-0.42661,0.12901,87.865,#6,-0.83536,0.19218,0.51502,146.98,0.31322,0.93634,0.15865,-129.07,-0.45175,0.29384,-0.84237,231.83,#3,0.84686,0.53103,0.029017,-110.99,-0.52691,0.84518,-0.089589,99.508,-0.0721,0.06058,0.99556,-18.965 |
| 5803 | | |
| 5804 | | > view matrix models |
| 5805 | | > #1,0.55394,0.75066,-0.36008,90.156,0.13096,0.34855,0.9281,101.72,0.82219,-0.56127,0.09477,98.841,#6,-0.93074,-0.26307,0.25401,292.43,-0.17738,0.9322,0.31551,-89.483,-0.31979,0.2486,-0.91429,235.23,#3,0.97925,0.042796,0.19808,-74.97,-0.048572,0.99852,0.024389,-39.72,-0.19674,-0.033504,0.97988,24.918 |
| 5806 | | |
| 5807 | | > view matrix models |
| 5808 | | > #1,0.5673,0.82189,-0.051582,77.636,0.043048,0.032954,0.99853,113.04,0.82238,-0.56869,-0.016686,102.58,#6,-0.93958,0.041873,0.33975,209.96,0.086342,0.98939,0.11684,-115.11,-0.33126,0.13911,-0.93323,265.4,#3,0.91484,0.35307,0.196,-123.47,-0.3723,0.92541,0.070709,22.647,-0.15642,-0.13766,0.97805,38.388 |
| 5809 | | |
| 5810 | | > view matrix models |
| 5811 | | > #1,0.49291,0.78746,-0.37005,96.979,0.077493,0.38389,0.92012,107.63,0.86662,-0.48221,0.1282,91.779,#6,-0.90459,-0.28436,0.31758,289.78,-0.15616,0.91426,0.37381,-92.017,-0.39665,0.28855,-0.87144,229.77,#3,0.99105,0.040677,0.12712,-61.789,-0.035989,0.99859,-0.038956,-28.701,-0.12853,0.034033,0.99112,-2.7827 |
| 5812 | | |
| 5813 | | > fitmap #3 inMap #2 |
| 5814 | | |
| 5815 | | Fit molecule hPNP_dimer_5zf6_fit_tetramer1.pdb (#3) to map |
| 5816 | | cryosparc_P1_J482_004_volume_map.mrc (#2) using 8555 atoms |
| 5817 | | average map value = 0.1145, steps = 1148 |
| 5818 | | shifted from previous position = 0.0468 |
| 5819 | | rotated from previous position = 5.6 degrees |
| 5820 | | atoms outside contour = 4238, contour level = 0.084608 |
| 5821 | | |
| 5822 | | Position of hPNP_dimer_5zf6_fit_tetramer1.pdb (#3) relative to |
| 5823 | | cryosparc_P1_J482_004_volume_map.mrc (#2) coordinates: |
| 5824 | | Matrix rotation and translation |
| 5825 | | 0.99191901 0.06352370 0.10982442 -62.76919098 |
| 5826 | | -0.06945569 0.99627767 0.05105578 -41.13025537 |
| 5827 | | -0.10617237 -0.05827113 0.99263885 10.89172991 |
| 5828 | | Axis -0.39581462 0.78200954 -0.48144768 |
| 5829 | | Axis point 2.83716672 0.00000000 617.02824957 |
| 5830 | | Rotation angle (degrees) 7.93812928 |
| 5831 | | Shift along axis -12.56308662 |
| 5832 | | |
| 5833 | | |
| 5834 | | > hide #!4 models |
| 5835 | | |
| 5836 | | > split #3 |
| 5837 | | |
| 5838 | | Split hPNP_dimer_5zf6_fit_tetramer1.pdb (#3) into 2 models |
| 5839 | | Chain information for hPNP_dimer_5zf6_fit_tetramer1.pdb A #3.1 |
| 5840 | | --- |
| 5841 | | Chain | Description |
| 5842 | | A | No description available |
| 5843 | | |
| 5844 | | Chain information for hPNP_dimer_5zf6_fit_tetramer1.pdb B #3.2 |
| 5845 | | --- |
| 5846 | | Chain | Description |
| 5847 | | B | No description available |
| 5848 | | |
| 5849 | | |
| 5850 | | > select add #3.2 |
| 5851 | | |
| 5852 | | 13214 atoms, 13318 bonds, 1808 residues, 3 models selected |
| 5853 | | |
| 5854 | | > select subtract #3.2 |
| 5855 | | |
| 5856 | | 8987 atoms, 9057 bonds, 1231 residues, 2 models selected |
| 5857 | | |
| 5858 | | > select add #3.1 |
| 5859 | | |
| 5860 | | 13315 atoms, 13414 bonds, 1828 residues, 3 models selected |
| 5861 | | |
| 5862 | | > view matrix models |
| 5863 | | > #1,0.63866,0.76924,-0.019308,72.947,-0.11384,0.11928,0.98631,131.14,0.76102,-0.62772,0.16375,104.77,#6,-0.96168,0.087149,0.25996,211.37,0.16026,0.94797,0.27507,-124.61,-0.22246,0.30619,-0.92561,212.03,#3.1,0.87837,0.37425,0.29732,-138.47,-0.38698,0.92193,-0.017224,45.309,-0.28055,-0.099926,0.95462,58.624 |
| 5864 | | |
| 5865 | | > ui mousemode right "translate selected models" |
| 5866 | | |
| 5867 | | > view matrix models |
| 5868 | | > #1,0.63866,0.76924,-0.019308,72.084,-0.11384,0.11928,0.98631,125.49,0.76102,-0.62772,0.16375,100.68,#6,-0.96168,0.087149,0.25996,210.51,0.16026,0.94797,0.27507,-130.26,-0.22246,0.30619,-0.92561,207.94,#3.1,0.87837,0.37425,0.29732,-139.33,-0.38698,0.92193,-0.017224,39.653,-0.28055,-0.099926,0.95462,54.532 |
| 5869 | | |
| 5870 | | > view matrix models |
| 5871 | | > #1,0.63866,0.76924,-0.019308,72.54,-0.11384,0.11928,0.98631,126.82,0.76102,-0.62772,0.16375,102,#6,-0.96168,0.087149,0.25996,210.96,0.16026,0.94797,0.27507,-128.93,-0.22246,0.30619,-0.92561,209.26,#3.1,0.87837,0.37425,0.29732,-138.87,-0.38698,0.92193,-0.017224,40.985,-0.28055,-0.099926,0.95462,55.849 |
| 5872 | | |
| 5873 | | > ui mousemode right "rotate selected models" |
| 5874 | | |
| 5875 | | > view matrix models |
| 5876 | | > #1,0.72286,0.68885,-0.054338,64.422,-0.094506,0.17646,0.97976,124.12,0.6845,-0.7031,0.19266,111.02,#6,-0.98739,0.06889,0.14254,227.23,0.11061,0.94433,0.30983,-125.74,-0.11326,0.32169,-0.94005,195.53,#3.1,0.86215,0.31108,0.39992,-145.84,-0.33042,0.94358,-0.021645,27.528,-0.38409,-0.11348,0.9163,85.446 |
| 5877 | | |
| 5878 | | > view matrix models |
| 5879 | | > #1,0.49021,0.82777,-0.27293,94.401,-0.20452,0.41364,0.88717,136.23,0.84727,-0.37908,0.37207,84.985,#6,-0.91222,-0.18982,0.36306,263.96,0.048035,0.83052,0.55491,-111.5,-0.40687,0.52364,-0.74851,162.13,#3.1,0.98015,0.16795,0.1053,-79.273,-0.14126,0.96443,-0.22342,31.317,-0.13908,0.20411,0.96902,-29.133 |
| 5880 | | |
| 5881 | | > fitmap #3.1 inMap #2 |
| 5882 | | |
| 5883 | | Fit molecule hPNP_dimer_5zf6_fit_tetramer1.pdb A (#3.1) to map |
| 5884 | | cryosparc_P1_J482_004_volume_map.mrc (#2) using 4328 atoms |
| 5885 | | average map value = 0.1678, steps = 136 |
| 5886 | | shifted from previous position = 3.69 |
| 5887 | | rotated from previous position = 14.6 degrees |
| 5888 | | atoms outside contour = 1115, contour level = 0.084608 |
| 5889 | | |
| 5890 | | Position of hPNP_dimer_5zf6_fit_tetramer1.pdb A (#3.1) relative to |
| 5891 | | cryosparc_P1_J482_004_volume_map.mrc (#2) coordinates: |
| 5892 | | Matrix rotation and translation |
| 5893 | | 0.99674815 0.00855406 -0.08012447 -16.33333536 |
| 5894 | | -0.03149945 0.95659060 -0.28972780 24.97205938 |
| 5895 | | 0.07416797 0.29130952 0.95374937 -82.05312532 |
| 5896 | | Axis 0.96436593 -0.25608401 -0.06647806 |
| 5897 | | Axis point 0.00000000 289.92437323 27.99933406 |
| 5898 | | Rotation angle (degrees) 17.53291000 |
| 5899 | | Shift along axis -16.69152470 |
| 5900 | | |
| 5901 | | |
| 5902 | | > volume #2 color #d783ff |
| 5903 | | |
| 5904 | | > transparency 50 |
| 5905 | | |
| 5906 | | > volume #2 level 0.1528 |
| 5907 | | |
| 5908 | | > fitmap #3.1 inMap #2 |
| 5909 | | |
| 5910 | | Fit molecule hPNP_dimer_5zf6_fit_tetramer1.pdb A (#3.1) to map |
| 5911 | | cryosparc_P1_J482_004_volume_map.mrc (#2) using 4328 atoms |
| 5912 | | average map value = 0.1678, steps = 48 |
| 5913 | | shifted from previous position = 0.00201 |
| 5914 | | rotated from previous position = 0.0151 degrees |
| 5915 | | atoms outside contour = 2432, contour level = 0.15284 |
| 5916 | | |
| 5917 | | Position of hPNP_dimer_5zf6_fit_tetramer1.pdb A (#3.1) relative to |
| 5918 | | cryosparc_P1_J482_004_volume_map.mrc (#2) coordinates: |
| 5919 | | Matrix rotation and translation |
| 5920 | | 0.99676224 0.00853600 -0.07995103 -16.36893175 |
| 5921 | | -0.03144631 0.95653366 -0.28992148 25.01195907 |
| 5922 | | 0.07400108 0.29149695 0.95370506 -82.04786196 |
| 5923 | | Axis 0.96455693 -0.25540226 -0.06632955 |
| 5924 | | Axis point 0.00000000 289.73990669 28.09020522 |
| 5925 | | Rotation angle (degrees) 17.54119628 |
| 5926 | | Shift along axis -16.73467989 |
| 5927 | | |
| 5928 | | Must specify one map, got 0 |
| 5929 | | |
| 5930 | | > correlation #3.1 #1 |
| 5931 | | |
| 5932 | | Unknown command: correlation #3.1 #1 |
| 5933 | | |
| 5934 | | > correlation |
| 5935 | | |
| 5936 | | Unknown command: correlation |
| 5937 | | |
| 5938 | | > crossfade correlation #3.1 #1 |
| 5939 | | |
| 5940 | | Expected an integer >= 1 or a keyword |
| 5941 | | |
| 5942 | | > crosscorrelation #3.1 #1 |
| 5943 | | |
| 5944 | | Unknown command: crosscorrelation #3.1 #1 |
| 5945 | | |
| 5946 | | > crosscorrelation #3.1 #1 |
| 5947 | | |
| 5948 | | Unknown command: measure crosscorrelation #3.1 #1 |
| 5949 | | |
| 5950 | | > volume #2 level 0.09062 |
| 5951 | | |
| 5952 | | Must specify one map, got 0 |
| 5953 | | Must specify one map, got 0 |
| 5954 | | |
| 5955 | | > save |
| 5956 | | > /Users/madhurikanavalli/Desktop/2025/Cryosparc/Q387R/tetramer_02_withouttickoncopymaps.cxs |
| 5957 | | > includeMaps true |
| 5958 | | |
| 5959 | | > combine #3.1 #3.2 |
| 5960 | | |
| 5961 | | Cell requested for row 6 is out of bounds for table with 9 rows! Resizing |
| 5962 | | table model. |
| 5963 | | |
| 5964 | | > select add #5 |
| 5965 | | |
| 5966 | | 21870 atoms, 22032 bonds, 3002 residues, 4 models selected |
| 5967 | | |
| 5968 | | > select subtract #4.1 |
| 5969 | | |
| 5970 | | 21870 atoms, 22032 bonds, 3002 residues, 4 models selected |
| 5971 | | |
| 5972 | | > hide #4.1 models |
| 5973 | | |
| 5974 | | > show #4.1 models |
| 5975 | | |
| 5976 | | > select subtract #4.1 |
| 5977 | | |
| 5978 | | 21870 atoms, 22032 bonds, 3002 residues, 4 models selected |
| 5979 | | |
| 5980 | | > select add #4 |
| 5981 | | |
| 5982 | | 30425 atoms, 30650 bonds, 4176 residues, 5 models selected |
| 5983 | | |
| 5984 | | > hide #!4 models |
| 5985 | | |
| 5986 | | > hide #4.1 models |
| 5987 | | |
| 5988 | | > show #!4 models |
| 5989 | | |
| 5990 | | > hide #!4 models |
| 5991 | | |
| 5992 | | > select subtract #5 |
| 5993 | | |
| 5994 | | 21870 atoms, 22032 bonds, 3002 residues, 4 models selected |
| 5995 | | |
| 5996 | | > hide #5 models |
| 5997 | | |
| 5998 | | > show #5 models |
| 5999 | | |
| 6000 | | > select add #5 |
| 6001 | | |
| 6002 | | 30425 atoms, 30650 bonds, 4176 residues, 5 models selected |
| 6003 | | |
| 6004 | | > select subtract #5 |
| 6005 | | |
| 6006 | | 21870 atoms, 22032 bonds, 3002 residues, 4 models selected |
| 6007 | | |
| 6008 | | > select add #5 |
| 6009 | | |
| 6010 | | 30425 atoms, 30650 bonds, 4176 residues, 5 models selected |
| 6011 | | |
| 6012 | | > select subtract #5 |
| 6013 | | |
| 6014 | | 21870 atoms, 22032 bonds, 3002 residues, 4 models selected |
| 6015 | | |
| 6016 | | > select add #5 |
| 6017 | | |
| 6018 | | 30425 atoms, 30650 bonds, 4176 residues, 5 models selected |
| 6019 | | |
| 6020 | | > select subtract #5 |
| 6021 | | |
| 6022 | | 21870 atoms, 22032 bonds, 3002 residues, 4 models selected |
| 6023 | | |
| 6024 | | > select add #5 |
| 6025 | | |
| 6026 | | 30425 atoms, 30650 bonds, 4176 residues, 5 models selected |
| 6027 | | |
| 6028 | | > hide #3.2 models |
| 6029 | | |
| 6030 | | > select subtract #5 |
| 6031 | | |
| 6032 | | 21870 atoms, 22032 bonds, 3002 residues, 4 models selected |
| 6033 | | |
| 6034 | | > select add #5 |
| 6035 | | |
| 6036 | | 30425 atoms, 30650 bonds, 4176 residues, 5 models selected |
| 6037 | | |
| 6038 | | > select subtract #5 |
| 6039 | | |
| 6040 | | 21870 atoms, 22032 bonds, 3002 residues, 4 models selected |
| 6041 | | |
| 6042 | | > select add #5 |
| 6043 | | |
| 6044 | | 30425 atoms, 30650 bonds, 4176 residues, 5 models selected |
| 6045 | | |
| 6046 | | > combine #3.1 #3.2 |
| 6047 | | |
| 6048 | | > save /Users/madhurikanavalli/Desktop/2025/Cryosparc/Q387R/9kjr_ChainA.pdb |
| 6049 | | > models #7 relModel #7 |
| 6050 | | |
| 6051 | | > save |
| 6052 | | > /Users/madhurikanavalli/Desktop/2025/Cryosparc/Q387R/tetramer_02_withouttickoncopymaps.cxs |
| 6053 | | > includeMaps true |
| 6054 | | |
| 6055 | | ——— End of log from Wed Nov 5 22:22:09 2025 ——— |
| 6056 | | |
| 6057 | | > view name session-start |
| 6058 | | |
| 6059 | | opened ChimeraX session |
| 6060 | | |
| 6061 | | > show #!4 models |
| 6062 | | |
| 6063 | | > split #420 |
| 6064 | | |
| 6065 | | > split #4 |
| 6066 | | |
| 6067 | | Split hPNP_dimer_5zf6_fit_tetramer2.pdb (#4) into 2 models |
| 6068 | | Chain information for hPNP_dimer_5zf6_fit_tetramer2.pdb A #4.1 |
| 6069 | | --- |
| 6070 | | Chain | Description |
| 6071 | | A | No description available |
| 6072 | | |
| 6073 | | Chain information for hPNP_dimer_5zf6_fit_tetramer2.pdb B #4.2 |
| 6074 | | --- |
| 6075 | | Chain | Description |
| 6076 | | B | No description available |
| 6077 | | |
| 6078 | | |
| 6079 | | > ui tool show "Fit in Map" |
| 6080 | | |
| 6081 | | > fitmap #4.1 inMap #2 |
| 6082 | | |
| 6083 | | Fit molecule hPNP_dimer_5zf6_fit_tetramer2.pdb A (#4.1) to map |
| 6084 | | cryosparc_P1_J482_004_volume_map.mrc (#2) using 4328 atoms |
| 6085 | | average map value = 0.1511, steps = 156 |
| 6086 | | shifted from previous position = 8.94 |
| 6087 | | rotated from previous position = 22.3 degrees |
| 6088 | | atoms outside contour = 1422, contour level = 0.090619 |
| 6089 | | |
| 6090 | | Position of hPNP_dimer_5zf6_fit_tetramer2.pdb A (#4.1) relative to |
| 6091 | | cryosparc_P1_J482_004_volume_map.mrc (#2) coordinates: |
| 6092 | | Matrix rotation and translation |
| 6093 | | 0.98383867 0.06125938 0.16825207 -71.95682366 |
| 6094 | | -0.00104748 0.94160854 -0.33670796 23.75307981 |
| 6095 | | -0.17905411 0.33109007 0.92645507 -46.53042246 |
| 6096 | | Axis 0.88416501 0.45983359 -0.08249432 |
| 6097 | | Axis point 0.00000000 176.40808408 106.68048095 |
| 6098 | | Rotation angle (degrees) 22.18778127 |
| 6099 | | Shift along axis -48.86074587 |
| 6100 | | |
| 6101 | | |
| 6102 | | > fitmap #4.2 inMap #2 |
| 6103 | | |
| 6104 | | Fit molecule hPNP_dimer_5zf6_fit_tetramer2.pdb B (#4.2) to map |
| 6105 | | cryosparc_P1_J482_004_volume_map.mrc (#2) using 4227 atoms |
| 6106 | | average map value = 0.1559, steps = 64 |
| 6107 | | shifted from previous position = 0.136 |
| 6108 | | rotated from previous position = 0.638 degrees |
| 6109 | | atoms outside contour = 1298, contour level = 0.090619 |
| 6110 | | |
| 6111 | | Position of hPNP_dimer_5zf6_fit_tetramer2.pdb B (#4.2) relative to |
| 6112 | | cryosparc_P1_J482_004_volume_map.mrc (#2) coordinates: |
| 6113 | | Matrix rotation and translation |
| 6114 | | 0.99994145 0.01081390 0.00039510 -39.96225052 |
| 6115 | | -0.01081776 0.99987385 0.01163050 -39.04984188 |
| 6116 | | -0.00026928 -0.01163409 0.99993229 -26.51523205 |
| 6117 | | Axis -0.73218070 0.02090926 -0.68078942 |
| 6118 | | Axis point 0.00000000 421.54756264 3445.69864868 |
| 6119 | | Rotation angle (degrees) 0.91030714 |
| 6120 | | Shift along axis 46.49437443 |
| 6121 | | |
| 6122 | | |
| 6123 | | > combine #4..1 #4.2 |
| 6124 | | |
| 6125 | | Expected a keyword |
| 6126 | | |
| 6127 | | > combine #4.1 #4.2 |
| 6128 | | |
| 6129 | | > select add #7 |
| 6130 | | |
| 6131 | | 30425 atoms, 30650 bonds, 4176 residues, 5 models selected |
| 6132 | | |
| 6133 | | > select subtract #7 |
| 6134 | | |
| 6135 | | 21870 atoms, 22032 bonds, 3002 residues, 4 models selected |
| 6136 | | |
| 6137 | | > select add #7 |
| 6138 | | |
| 6139 | | 30425 atoms, 30650 bonds, 4176 residues, 5 models selected |
| 6140 | | |
| 6141 | | > select subtract #7 |
| 6142 | | |
| 6143 | | 21870 atoms, 22032 bonds, 3002 residues, 4 models selected |
| 6144 | | |
| 6145 | | > select subtract #6 |
| 6146 | | |
| 6147 | | 13315 atoms, 13414 bonds, 1828 residues, 3 models selected |
| 6148 | | |
| 6149 | | > select subtract #5 |
| 6150 | | |
| 6151 | | 4760 atoms, 4796 bonds, 654 residues, 2 models selected |
| 6152 | | |
| 6153 | | > select add #1 |
| 6154 | | |
| 6155 | | 12883 atoms, 12975 bonds, 1771 residues, 2 models selected |
| 6156 | | |
| 6157 | | > select add #3 |
| 6158 | | |
| 6159 | | 17110 atoms, 17236 bonds, 2348 residues, 4 models selected |
| 6160 | | |
| 6161 | | > select subtract #3 |
| 6162 | | |
| 6163 | | 8555 atoms, 8618 bonds, 1174 residues, 1 model selected |
| 6164 | | |
| 6165 | | > select subtract #1 |
| 6166 | | |
| 6167 | | Nothing selected |
| 6168 | | |
| 6169 | | > select add #5 |
| 6170 | | |
| 6171 | | 8555 atoms, 8618 bonds, 1174 residues, 1 model selected |
| 6172 | | |
| 6173 | | > select add #8 |
| 6174 | | |
| 6175 | | 17110 atoms, 17236 bonds, 2348 residues, 2 models selected |
| 6176 | | |
| 6177 | | > combine #5 #8 |
| 6178 | | |
| 6179 | | Remapping chain ID 'A' in combination #8 to 'C' |
| 6180 | | Remapping chain ID 'B' in combination #8 to 'D' |
| 6181 | | |
| 6182 | | > select subtract #8 |
| 6183 | | |
| 6184 | | 8555 atoms, 8618 bonds, 1174 residues, 1 model selected |
| 6185 | | |
| 6186 | | > select subtract #5 |
| 6187 | | |
| 6188 | | Nothing selected |
| 6189 | | |
| 6190 | | > select add #7 |
| 6191 | | |
| 6192 | | 8555 atoms, 8618 bonds, 1174 residues, 1 model selected |
| 6193 | | |
| 6194 | | > select subtract #7 |
| 6195 | | |
| 6196 | | Nothing selected |
| 6197 | | |
| 6198 | | > select add #7 |
| 6199 | | |
| 6200 | | 8555 atoms, 8618 bonds, 1174 residues, 1 model selected |
| 6201 | | |
| 6202 | | > select subtract #7 |
| 6203 | | |
| 6204 | | Nothing selected |
| 6205 | | |
| 6206 | | > select add #9 |
| 6207 | | |
| 6208 | | 17110 atoms, 17236 bonds, 2348 residues, 1 model selected |
| 6209 | | |
| 6210 | | > select subtract #9 |
| 6211 | | |
| 6212 | | Nothing selected |
| 6213 | | |
| 6214 | | > close #1 |
| 6215 | | |
| 6216 | | > close #3 |
| 6217 | | |
| 6218 | | > close #4 |
| 6219 | | |
| 6220 | | > hide #5 models |
| 6221 | | |
| 6222 | | > close #5 |
| 6223 | | |
| 6224 | | > show #!6 models |
| 6225 | | |
| 6226 | | > hide #!6 models |
| 6227 | | |
| 6228 | | > close #6 |
| 6229 | | |
| 6230 | | > hide #7 models |
| 6231 | | |
| 6232 | | > show #7 models |
| 6233 | | |
| 6234 | | > select add #7 |
| 6235 | | |
| 6236 | | 8555 atoms, 8618 bonds, 1174 residues, 1 model selected |
| 6237 | | |
| 6238 | | > select subtract #7 |
| 6239 | | |
| 6240 | | Nothing selected |
| 6241 | | |
| 6242 | | > select add #7 |
| 6243 | | |
| 6244 | | 8555 atoms, 8618 bonds, 1174 residues, 1 model selected |
| 6245 | | |
| 6246 | | > select subtract #7 |
| 6247 | | |
| 6248 | | Nothing selected |
| 6249 | | |
| 6250 | | > close #7 |
| 6251 | | |
| 6252 | | > select add #8 |
| 6253 | | |
| 6254 | | 8555 atoms, 8618 bonds, 1174 residues, 1 model selected |
| 6255 | | |
| 6256 | | > select subtract #8 |
| 6257 | | |
| 6258 | | Nothing selected |
| 6259 | | |
| 6260 | | > close #8 |
| 6261 | | |
| 6262 | | > select add #9 |
| 6263 | | |
| 6264 | | 17110 atoms, 17236 bonds, 2348 residues, 1 model selected |
| 6265 | | |
| 6266 | | > select subtract #9 |
| 6267 | | |
| 6268 | | Nothing selected |
| 6269 | | |
| 6270 | | > save |
| 6271 | | > /Users/madhurikanavalli/Desktop/2025/Cryosparc/Q387R/tetramerfit_J482_goodone.pdb |
| 6272 | | > relModel #2 |
| 6273 | | |
| 6274 | | > save |
| 6275 | | > /Users/madhurikanavalli/Desktop/2025/Cryosparc/Q387R/tetramerfit_J482_good02.pdb |
| 6276 | | > relModel #9 |
| 6277 | | |
| 6278 | | > save |
| 6279 | | > /Users/madhurikanavalli/Desktop/2025/Cryosparc/Q387R/Tetramer_goodmodel_01_05Nov25.cxs |
| 6280 | | > includeMaps true |
| 6281 | | |
| 6282 | | ——— End of log from Wed Nov 5 23:47:55 2025 ——— |
| 6283 | | |
| 6284 | | > view name session-start |
| 6285 | | |
| 6286 | | opened ChimeraX session |
| 6287 | | |
| 6288 | | > hide #9 models |
| 6289 | | |
| 6290 | | > ui tool show "Fit Loops" |
| 6291 | | |
| 6292 | | > show #9 models |
| 6293 | | |
| 6294 | | > ui tool show "Fit Loops" |
| 6295 | | |
| 6296 | | No selected amino-acid residues or missing-structure pseudobonds in |
| 6297 | | combination #9 |
| 6298 | | |
| 6299 | | > open |
| 6300 | | > /Users/madhurikanavalli/Downloads/fold_s1domain/fold_s1domain_model_0.cif |
| 6301 | | > /Users/madhurikanavalli/Downloads/fold_s1domain/fold_s1domain_model_1.cif |
| 6302 | | > /Users/madhurikanavalli/Downloads/fold_s1domain/fold_s1domain_model_2.cif |
| 6303 | | > /Users/madhurikanavalli/Downloads/fold_s1domain/fold_s1domain_model_3.cif |
| 6304 | | > /Users/madhurikanavalli/Downloads/fold_s1domain/fold_s1domain_model_4.cif |
| 6305 | | |
| 6306 | | Chain information for fold_s1domain_model_0.cif #1 |
| 6307 | | --- |
| 6308 | | Chain | Description |
| 6309 | | A | . |
| 6310 | | |
| 6311 | | Chain information for fold_s1domain_model_1.cif #3 |
| 6312 | | --- |
| 6313 | | Chain | Description |
| 6314 | | A | . |
| 6315 | | |
| 6316 | | Chain information for fold_s1domain_model_2.cif #4 |
| 6317 | | --- |
| 6318 | | Chain | Description |
| 6319 | | A | . |
| 6320 | | |
| 6321 | | Chain information for fold_s1domain_model_3.cif #5 |
| 6322 | | --- |
| 6323 | | Chain | Description |
| 6324 | | A | . |
| 6325 | | |
| 6326 | | Chain information for fold_s1domain_model_4.cif #6 |
| 6327 | | --- |
| 6328 | | Chain | Description |
| 6329 | | A | . |
| 6330 | | |
| 6331 | | Computing secondary structure |
| 6332 | | [Repeated 4 time(s)] |
| 6333 | | |
| 6334 | | > hide #9 models |
| 6335 | | |
| 6336 | | > tile |
| 6337 | | |
| 6338 | | 6 models tiled |
| 6339 | | |
| 6340 | | > hide #!2 models |
| 6341 | | |
| 6342 | | > show #!2 models |
| 6343 | | |
| 6344 | | > color #3 #7a81ffff |
| 6345 | | |
| 6346 | | > color #3 #0433ffff |
| 6347 | | |
| 6348 | | > select add #3 |
| 6349 | | |
| 6350 | | 522 atoms, 529 bonds, 65 residues, 1 model selected |
| 6351 | | |
| 6352 | | > view matrix models #3,1,0,0,167.58,0,1,0,54.796,0,0,1,204.78 |
| 6353 | | |
| 6354 | | > view matrix models #3,1,0,0,165.39,0,1,0,115.67,0,0,1,225.25 |
| 6355 | | |
| 6356 | | > ui mousemode right "move picked models" |
| 6357 | | |
| 6358 | | > view matrix models #2,1,0,0,-2.0304,0,1,0,-3.9698,0,0,1,-16.281 |
| 6359 | | |
| 6360 | | > view matrix models #2,1,0,0,-2.6783,0,1,0,2.0885,0,0,1,-17.199 |
| 6361 | | |
| 6362 | | > ui mousemode right "rotate selected models" |
| 6363 | | |
| 6364 | | > view matrix models |
| 6365 | | > #3,0.32589,0.91906,-0.22164,161.57,-0.87579,0.20518,-0.43692,116.43,-0.35608,0.3365,0.87177,223.66 |
| 6366 | | |
| 6367 | | > view matrix models |
| 6368 | | > #3,0.56547,-0.7859,-0.25021,166.9,0.82291,0.55799,0.10713,118.68,0.055423,-0.26647,0.96225,226.17 |
| 6369 | | |
| 6370 | | > ui mousemode right "translate selected models" |
| 6371 | | |
| 6372 | | > view matrix models |
| 6373 | | > #3,0.56547,-0.7859,-0.25021,163.29,0.82291,0.55799,0.10713,131.17,0.055423,-0.26647,0.96225,208.64 |
| 6374 | | |
| 6375 | | > view matrix models |
| 6376 | | > #3,0.29541,0.69457,0.65597,155.91,-0.92197,0.38722,0.0051959,144.72,-0.2504,-0.60633,0.75477,209.92 |
| 6377 | | |
| 6378 | | > view matrix models |
| 6379 | | > #3,0.79695,-0.604,0.0075442,161.41,0.52088,0.6935,0.49774,151.3,-0.30587,-0.39275,0.86729,210.32 |
| 6380 | | |
| 6381 | | > view matrix models |
| 6382 | | > #3,0.46843,-0.85689,-0.21519,160.99,0.82693,0.33948,0.44827,160.63,-0.31106,-0.38793,0.86761,210.1 |
| 6383 | | |
| 6384 | | > view matrix models |
| 6385 | | > #3,-0.34505,0.91726,0.19892,154.61,-0.88742,-0.24981,-0.38741,149.7,-0.30566,-0.3102,0.90019,211.43 |
| 6386 | | |
| 6387 | | > view matrix models |
| 6388 | | > #3,0.66119,0.61943,0.42324,159.05,-0.59559,0.77644,-0.20593,116.72,-0.45618,-0.11592,0.8823,203.44 |
| 6389 | | |
| 6390 | | > ui tool show "Fit in Map" |
| 6391 | | |
| 6392 | | > fitmap #1 inMap #2 |
| 6393 | | |
| 6394 | | Fit molecule fold_s1domain_model_0.cif (#1) to map |
| 6395 | | cryosparc_P1_J482_004_volume_map.mrc (#2) using 522 atoms |
| 6396 | | average map value = 0.0003632, steps = 356 |
| 6397 | | shifted from previous position = 4.22 |
| 6398 | | rotated from previous position = 14.5 degrees |
| 6399 | | atoms outside contour = 522, contour level = 0.090619 |
| 6400 | | |
| 6401 | | Position of fold_s1domain_model_0.cif (#1) relative to |
| 6402 | | cryosparc_P1_J482_004_volume_map.mrc (#2) coordinates: |
| 6403 | | Matrix rotation and translation |
| 6404 | | 0.97213948 0.21632806 0.09026078 182.36500636 |
| 6405 | | -0.20718521 0.97309809 -0.10076905 2.02323771 |
| 6406 | | -0.10963177 0.07926087 0.99080704 169.96457072 |
| 6407 | | Axis 0.35881954 0.39840796 -0.84410878 |
| 6408 | | Axis point 279.68301149 -935.07624490 0.00000000 |
| 6409 | | Rotation angle (degrees) 14.52865421 |
| 6410 | | Shift along axis -77.22638618 |
| 6411 | | |
| 6412 | | |
| 6413 | | > fitmap #1 inMap #2 |
| 6414 | | |
| 6415 | | Fit molecule fold_s1domain_model_0.cif (#1) to map |
| 6416 | | cryosparc_P1_J482_004_volume_map.mrc (#2) using 522 atoms |
| 6417 | | average map value = 0.000365, steps = 100 |
| 6418 | | shifted from previous position = 0.137 |
| 6419 | | rotated from previous position = 0.599 degrees |
| 6420 | | atoms outside contour = 522, contour level = 0.090619 |
| 6421 | | |
| 6422 | | Position of fold_s1domain_model_0.cif (#1) relative to |
| 6423 | | cryosparc_P1_J482_004_volume_map.mrc (#2) coordinates: |
| 6424 | | Matrix rotation and translation |
| 6425 | | 0.97288387 0.20994836 0.09704977 182.50082645 |
| 6426 | | -0.19979031 0.97422533 -0.10473222 2.03411083 |
| 6427 | | -0.11653670 0.08250268 0.98975376 169.89241874 |
| 6428 | | Axis 0.37555154 0.42840695 -0.82184459 |
| 6429 | | Axis point 284.35086060 -958.13936528 0.00000000 |
| 6430 | | Rotation angle (degrees) 14.43490420 |
| 6431 | | Shift along axis -70.21527182 |
| 6432 | | |
| 6433 | | |
| 6434 | | > fitmap #3 inMap #2 |
| 6435 | | |
| 6436 | | Fit molecule fold_s1domain_model_1.cif (#3) to map |
| 6437 | | cryosparc_P1_J482_004_volume_map.mrc (#2) using 522 atoms |
| 6438 | | average map value = 0.07067, steps = 164 |
| 6439 | | shifted from previous position = 8.32 |
| 6440 | | rotated from previous position = 39.5 degrees |
| 6441 | | atoms outside contour = 363, contour level = 0.090619 |
| 6442 | | |
| 6443 | | Position of fold_s1domain_model_1.cif (#3) relative to |
| 6444 | | cryosparc_P1_J482_004_volume_map.mrc (#2) coordinates: |
| 6445 | | Matrix rotation and translation |
| 6446 | | 0.92043171 -0.33817014 -0.19607755 159.00664102 |
| 6447 | | 0.31899036 0.93970634 -0.12327665 125.40584612 |
| 6448 | | 0.22594381 0.05092090 0.97280854 218.61730845 |
| 6449 | | Axis 0.21769478 -0.52740033 0.82125385 |
| 6450 | | Axis point -542.84900192 502.64006802 0.00000000 |
| 6451 | | Rotation angle (degrees) 23.58417039 |
| 6452 | | Shift along axis 148.01613600 |
| 6453 | | |
| 6454 | | |
| 6455 | | > fitmap #4 inMap #2 |
| 6456 | | |
| 6457 | | Fit molecule fold_s1domain_model_2.cif (#4) to map |
| 6458 | | cryosparc_P1_J482_004_volume_map.mrc (#2) using 522 atoms |
| 6459 | | average map value = -0.001537, steps = 80 |
| 6460 | | shifted from previous position = 0.947 |
| 6461 | | rotated from previous position = 11.5 degrees |
| 6462 | | atoms outside contour = 522, contour level = 0.090619 |
| 6463 | | |
| 6464 | | Position of fold_s1domain_model_2.cif (#4) relative to |
| 6465 | | cryosparc_P1_J482_004_volume_map.mrc (#2) coordinates: |
| 6466 | | Matrix rotation and translation |
| 6467 | | 0.98137074 -0.01475330 -0.19155629 124.31223336 |
| 6468 | | 0.00382580 0.99835020 -0.05729090 166.97625539 |
| 6469 | | 0.19208549 0.05549076 0.97980811 25.02135082 |
| 6470 | | Axis 0.28173775 -0.95836828 0.04641208 |
| 6471 | | Axis point -72.08236990 0.00000000 838.11125119 |
| 6472 | | Rotation angle (degrees) 11.54594279 |
| 6473 | | Shift along axis -123.84000488 |
| 6474 | | |
| 6475 | | |
| 6476 | | > volume #2 level 0.07472 |
| 6477 | | |
| 6478 | | > view matrix models |
| 6479 | | > #3,-0.67188,0.62554,0.39658,156.11,-0.55661,-0.77969,0.28683,98.367,0.48863,-0.028029,0.87204,213.25 |
| 6480 | | |
| 6481 | | > style sel ball |
| 6482 | | |
| 6483 | | Changed 522 atom styles |
| 6484 | | |
| 6485 | | > style sel sphere |
| 6486 | | |
| 6487 | | Changed 522 atom styles |
| 6488 | | |
| 6489 | | > show sel surfaces |
| 6490 | | |
| 6491 | | > view matrix models |
| 6492 | | > #3,-0.76709,0.51052,0.38851,156.55,-0.43671,-0.85915,0.26672,89.922,0.46996,0.034932,0.882,210.08 |
| 6493 | | |
| 6494 | | > ui mousemode right "rotate selected models" |
| 6495 | | |
| 6496 | | > view matrix models |
| 6497 | | > #3,-0.7899,0.60735,-0.084731,156.46,-0.59625,-0.72837,0.33758,88.914,0.14331,0.31717,0.93748,208.03 |
| 6498 | | |
| 6499 | | > view matrix models |
| 6500 | | > #3,-0.66024,0.41001,0.62926,157.09,-0.37315,-0.90619,0.19893,90.332,0.6518,-0.10346,0.7513,211.23 |
| 6501 | | |
| 6502 | | > view matrix models |
| 6503 | | > #3,0.35613,-0.51243,-0.7814,164.32,-0.36034,0.69624,-0.62081,86.3,0.86216,0.50267,0.063305,210.69 |
| 6504 | | |
| 6505 | | > view matrix models |
| 6506 | | > #3,0.22649,-0.61697,-0.75369,164.12,-0.74129,0.39275,-0.54427,85.701,0.63181,0.68198,-0.3684,209.58 |
| 6507 | | |
| 6508 | | > view matrix models |
| 6509 | | > #3,0.4365,-0.66898,-0.60161,164.96,-0.60804,0.27351,-0.74531,86.654,0.66314,0.69112,-0.28738,209.62 |
| 6510 | | |
| 6511 | | > hide sel surfaces |
| 6512 | | |
| 6513 | | > view matrix models |
| 6514 | | > #3,0.19536,-0.53018,-0.82507,164.33,-0.68664,0.52674,-0.50106,85.416,0.70026,0.66442,-0.26114,209.74 |
| 6515 | | |
| 6516 | | > view matrix models |
| 6517 | | > #3,0.35707,-0.66519,-0.65576,164.84,-0.63986,0.33727,-0.69053,86.324,0.6805,0.66617,-0.3052,209.76 |
| 6518 | | |
| 6519 | | > view matrix models |
| 6520 | | > #3,0.3911,-0.62689,-0.67384,164.84,-0.65268,0.32728,-0.6833,86.311,0.64888,0.70704,-0.28116,209.53 |
| 6521 | | |
| 6522 | | > view matrix models |
| 6523 | | > #3,0.50296,-0.58623,-0.63512,164.94,-0.61625,0.27201,-0.73908,86.63,0.60603,0.76312,-0.22446,209.2 |
| 6524 | | |
| 6525 | | > ui mousemode right "translate selected models" |
| 6526 | | |
| 6527 | | > view matrix models |
| 6528 | | > #3,0.50296,-0.58623,-0.63512,159.63,-0.61625,0.27201,-0.73908,149.03,0.60603,0.76312,-0.22446,209.13 |
| 6529 | | |
| 6530 | | > view matrix models |
| 6531 | | > #3,0.50296,-0.58623,-0.63512,160.92,-0.61625,0.27201,-0.73908,148.39,0.60603,0.76312,-0.22446,217.66 |
| 6532 | | |
| 6533 | | > view matrix models |
| 6534 | | > #3,0.50296,-0.58623,-0.63512,160.7,-0.61625,0.27201,-0.73908,148.47,0.60603,0.76312,-0.22446,216.16 |
| 6535 | | |
| 6536 | | > view matrix models |
| 6537 | | > #3,-0.45496,0.52695,-0.71787,152.27,-0.45166,0.55821,0.696,166.64,0.76747,0.64088,-0.015958,206.6 |
| 6538 | | |
| 6539 | | > fitmap #4 inMap #2 |
| 6540 | | |
| 6541 | | Fit molecule fold_s1domain_model_2.cif (#4) to map |
| 6542 | | cryosparc_P1_J482_004_volume_map.mrc (#2) using 522 atoms |
| 6543 | | average map value = -0.0004006, steps = 88 |
| 6544 | | shifted from previous position = 1.25 |
| 6545 | | rotated from previous position = 6.05 degrees |
| 6546 | | atoms outside contour = 522, contour level = 0.074721 |
| 6547 | | |
| 6548 | | Position of fold_s1domain_model_2.cif (#4) relative to |
| 6549 | | cryosparc_P1_J482_004_volume_map.mrc (#2) coordinates: |
| 6550 | | Matrix rotation and translation |
| 6551 | | 0.99147668 0.01049450 -0.12986092 116.82021270 |
| 6552 | | -0.00708136 0.99961796 0.02671695 137.03591507 |
| 6553 | | 0.13009169 -0.02556964 0.99117221 26.84154122 |
| 6554 | | Axis -0.19675787 -0.97821877 -0.06613911 |
| 6555 | | Axis point -80.51316555 0.00000000 664.24143651 |
| 6556 | | Rotation angle (degrees) 7.63549261 |
| 6557 | | Shift along axis -158.81167600 |
| 6558 | | |
| 6559 | | |
| 6560 | | > fitmap #3 inMap #2 |
| 6561 | | |
| 6562 | | Fit molecule fold_s1domain_model_1.cif (#3) to map |
| 6563 | | cryosparc_P1_J482_004_volume_map.mrc (#2) using 522 atoms |
| 6564 | | average map value = 0.07284, steps = 144 |
| 6565 | | shifted from previous position = 9.14 |
| 6566 | | rotated from previous position = 32.2 degrees |
| 6567 | | atoms outside contour = 318, contour level = 0.074721 |
| 6568 | | |
| 6569 | | Position of fold_s1domain_model_1.cif (#3) relative to |
| 6570 | | cryosparc_P1_J482_004_volume_map.mrc (#2) coordinates: |
| 6571 | | Matrix rotation and translation |
| 6572 | | -0.51872333 0.27009097 -0.81115779 162.49967762 |
| 6573 | | -0.72798453 0.35795974 0.58472501 168.27559607 |
| 6574 | | 0.44829078 0.89382084 0.01093998 212.26586284 |
| 6575 | | Axis 0.18888415 -0.76963145 -0.60991001 |
| 6576 | | Axis point 77.24806995 0.00000000 108.85630195 |
| 6577 | | Rotation angle (degrees) 125.09345631 |
| 6578 | | Shift along axis -228.27965129 |
| 6579 | | |
| 6580 | | |
| 6581 | | > view matrix models |
| 6582 | | > #3,0.87466,-0.27955,-0.396,157.61,-0.41844,-0.023029,-0.90795,126.96,0.2447,0.95985,-0.13712,199.83 |
| 6583 | | |
| 6584 | | > view matrix models |
| 6585 | | > #3,0.7064,-0.4253,0.56579,157.56,0.3865,-0.43789,-0.81171,108.84,0.59297,0.79207,-0.14495,197.91 |
| 6586 | | |
| 6587 | | > fitmap #3 inMap #2 |
| 6588 | | |
| 6589 | | Fit molecule fold_s1domain_model_1.cif (#3) to map |
| 6590 | | cryosparc_P1_J482_004_volume_map.mrc (#2) using 522 atoms |
| 6591 | | average map value = 0.057, steps = 140 |
| 6592 | | shifted from previous position = 8.64 |
| 6593 | | rotated from previous position = 40 degrees |
| 6594 | | atoms outside contour = 355, contour level = 0.074721 |
| 6595 | | |
| 6596 | | Position of fold_s1domain_model_1.cif (#3) relative to |
| 6597 | | cryosparc_P1_J482_004_volume_map.mrc (#2) coordinates: |
| 6598 | | Matrix rotation and translation |
| 6599 | | -0.87064244 -0.19188813 -0.45294667 134.31464498 |
| 6600 | | -0.49130252 0.29321200 0.82015154 169.18135869 |
| 6601 | | -0.02456795 0.93659258 -0.34955793 220.00413042 |
| 6602 | | Axis 0.21746043 -0.80002225 -0.55917382 |
| 6603 | | Axis point 82.32214637 0.00000000 66.57043206 |
| 6604 | | Rotation angle (degrees) 164.47081111 |
| 6605 | | Shift along axis -229.16128017 |
| 6606 | | |
| 6607 | | |
| 6608 | | > view matrix models |
| 6609 | | > #3,0.95229,0.22388,0.20741,154.09,-0.28611,0.41832,0.86206,83.875,0.10624,-0.88028,0.46241,224.1 |
| 6610 | | |
| 6611 | | > open Q8TCS8 fromDatabase alphafold_pae format pae |
| 6612 | | |
| 6613 | | Fetching an AlphaFold database PAE file requires specifying the structure to |
| 6614 | | associate. For example "open P29474 from alphafold_pae structure #1" |
| 6615 | | |
| 6616 | | > open /Users/madhurikanavalli/Downloads/AF-Q8TCS8-F1-model_v6.pdb |
| 6617 | | |
| 6618 | | AF-Q8TCS8-F1-model_v6.pdb title: |
| 6619 | | Alphafold monomer V2.0 prediction for polyribonucleotide |
| 6620 | | nucleotidyltransferase 1, mitochondrial (Q8TCS8) [more info...] |
| 6621 | | |
| 6622 | | Chain information for AF-Q8TCS8-F1-model_v6.pdb #7 |
| 6623 | | --- |
| 6624 | | Chain | Description | UniProt |
| 6625 | | A | polyribonucleotide nucleotidyltransferase 1, mitochondrial | PNPT1_HUMAN 1-783 |
| 6626 | | |
| 6627 | | Computing secondary structure |
| 6628 | | |
| 6629 | | > hide #!3 models |
| 6630 | | |
| 6631 | | > select subtract #3 |
| 6632 | | |
| 6633 | | 1 model selected |
| 6634 | | |
| 6635 | | > hide #6 models |
| 6636 | | |
| 6637 | | > hide #!2 models |
| 6638 | | |
| 6639 | | > show #!2 models |
| 6640 | | |
| 6641 | | > hide #1 models |
| 6642 | | |
| 6643 | | > close #1 |
| 6644 | | |
| 6645 | | > close #6 |
| 6646 | | |
| 6647 | | > close #5 |
| 6648 | | |
| 6649 | | > close #4 |
| 6650 | | |
| 6651 | | > select add #7 |
| 6652 | | |
| 6653 | | 6032 atoms, 6129 bonds, 783 residues, 1 model selected |
| 6654 | | |
| 6655 | | > view matrix models |
| 6656 | | > #7,0.93203,-0.33526,0.13754,27.703,-0.3055,-0.5228,0.79584,135.27,-0.19491,-0.78376,-0.58968,172.48 |
| 6657 | | |
| 6658 | | > view matrix models |
| 6659 | | > #7,0.99383,0.081244,-0.075482,18.858,0.10523,-0.47615,0.87304,113.59,0.034988,-0.8756,-0.48177,159.02 |
| 6660 | | |
| 6661 | | > ui mousemode right "rotate selected models" |
| 6662 | | |
| 6663 | | > ui mousemode right "move picked models" |
| 6664 | | |
| 6665 | | > view matrix models |
| 6666 | | > #7,0.99383,0.081244,-0.075482,17.188,0.10523,-0.47615,0.87304,122.02,0.034988,-0.8756,-0.48177,152.46 |
| 6667 | | |
| 6668 | | > ui mousemode right "translate selected atoms" |
| 6669 | | |
| 6670 | | > ui mousemode right "move picked models" |
| 6671 | | |
| 6672 | | > view matrix models |
| 6673 | | > #2,-0.96007,-0.2796,-0.0089378,326.83,0.13649,-0.44028,-0.88742,273.98,0.24419,-0.85321,0.46087,83.166 |
| 6674 | | |
| 6675 | | > ui mousemode right "translate selected models" |
| | 1718 | [deleted to fit within ticket limits] |