| 2424 | | > view matrix models |
| 2425 | | > #11,-0.88887,-0.45801,0.011781,346.86,-0.23966,0.48671,0.84005,-8.5049,-0.39048,0.74387,-0.54239,168.45,#12,-0.88887,-0.45801,0.011781,346.86,-0.23966,0.48671,0.84005,-8.5049,-0.39048,0.74387,-0.54239,168.45 |
| 2426 | | |
| 2427 | | > view matrix models |
| 2428 | | > #11,-0.19168,-0.96873,-0.15754,339.31,-0.75924,0.044642,0.64928,159.39,-0.62194,0.24407,-0.74406,302.98,#12,-0.19168,-0.96873,-0.15754,339.31,-0.75924,0.044642,0.64928,159.39,-0.62194,0.24407,-0.74406,302.98 |
| 2429 | | |
| 2430 | | > fitmap #11 inMap #1 |
| 2431 | | |
| 2432 | | Fit map |
| 2433 | | W20_J3989_004_volume_map_csparc_refine_of_relion_job127_gammazeta_mask.mrc in |
| 2434 | | map W20_J3623_consensus_structure_sharp.mrc using 42734 points |
| 2435 | | correlation = 0.9154, correlation about mean = 0.4456, overlap = 5.735e+04 |
| 2436 | | steps = 104, shift = 9.56, angle = 10.3 degrees |
| 2437 | | |
| 2438 | | Position of |
| 2439 | | W20_J3989_004_volume_map_csparc_refine_of_relion_job127_gammazeta_mask.mrc |
| 2440 | | (#11) relative to W20_J3623_consensus_structure_sharp.mrc (#1) coordinates: |
| 2441 | | Matrix rotation and translation |
| 2442 | | -0.34146109 -0.91804101 -0.20150685 354.32329233 |
| 2443 | | -0.77726306 0.15526879 0.60971610 146.26157419 |
| 2444 | | -0.52845666 0.36481815 -0.76657763 277.10408311 |
| 2445 | | Axis -0.56679376 0.75669526 0.32581761 |
| 2446 | | Axis point 251.95105256 0.00000000 81.46580342 |
| 2447 | | Rotation angle (degrees) 167.52357003 |
| 2448 | | Shift along axis 0.13259978 |
| 2449 | | |
| 2450 | | |
| 2451 | | > fitmap #12 inMap #1 |
| 2452 | | |
| 2453 | | Fit map |
| 2454 | | W20_J3989_004_volume_map_sharp_csparc_refine_of_relion_job127_gammazeta_mask.mrc |
| 2455 | | in map W20_J3623_consensus_structure_sharp.mrc using 37926 points |
| 2456 | | correlation = 0.9185, correlation about mean = 0.4316, overlap = 5.437e+04 |
| 2457 | | steps = 120, shift = 9.52, angle = 10.3 degrees |
| 2458 | | |
| 2459 | | Position of |
| 2460 | | W20_J3989_004_volume_map_sharp_csparc_refine_of_relion_job127_gammazeta_mask.mrc |
| 2461 | | (#12) relative to W20_J3623_consensus_structure_sharp.mrc (#1) coordinates: |
| 2462 | | Matrix rotation and translation |
| 2463 | | -0.34132328 -0.91808135 -0.20155652 354.32310537 |
| 2464 | | -0.77719944 0.15505915 0.60985055 146.27901701 |
| 2465 | | -0.52863923 0.36480581 -0.76645762 277.14013363 |
| 2466 | | Axis -0.56684797 0.75662172 0.32589408 |
| 2467 | | Axis point 251.98254766 0.00000000 81.45327260 |
| 2468 | | Rotation angle (degrees) 167.51718440 |
| 2469 | | Shift along axis 0.14887713 |
| 2470 | | |
| 2471 | | |
| 2472 | | > select subtract #12 |
| 2473 | | |
| 2474 | | 2 models selected |
| 2475 | | |
| 2476 | | > select subtract #11 |
| 2477 | | |
| 2478 | | Nothing selected |
| 2479 | | |
| 2480 | | > hide #!1 models |
| 2481 | | |
| 2482 | | > volume #11 level 1.414 |
| 2483 | | |
| 2484 | | > hide #!11 models |
| 2485 | | |
| 2486 | | > hide #!12 models |
| 2487 | | |
| 2488 | | > show #!13 models |
| 2489 | | |
| 2490 | | > show #!1 models |
| 2491 | | |
| 2492 | | > show #!14 models |
| 2493 | | |
| 2494 | | > fitmap #13 inMap #1 |
| 2495 | | |
| 2496 | | Fit map W20_J4021_005_volume_map_bprime_local.mrc in map |
| 2497 | | W20_J3623_consensus_structure_sharp.mrc using 138166 points |
| 2498 | | correlation = 0.8362, correlation about mean = 0.2198, overlap = 1.935e+04 |
| 2499 | | steps = 64, shift = 0.521, angle = 0.419 degrees |
| 2500 | | |
| 2501 | | Position of W20_J4021_005_volume_map_bprime_local.mrc (#13) relative to |
| 2502 | | W20_J3623_consensus_structure_sharp.mrc (#1) coordinates: |
| 2503 | | Matrix rotation and translation |
| 2504 | | 0.99998250 0.00590533 0.00034832 -0.90323251 |
| 2505 | | -0.00590377 0.99997332 -0.00430079 1.11548441 |
| 2506 | | -0.00037371 0.00429865 0.99999069 -0.31823043 |
| 2507 | | Axis 0.58794667 0.04936530 -0.80739196 |
| 2508 | | Axis point 190.18082923 131.70665579 0.00000000 |
| 2509 | | Rotation angle (degrees) 0.41901428 |
| 2510 | | Shift along axis -0.21904963 |
| 2511 | | |
| 2512 | | |
| 2513 | | > fitmap #14 inMap #1 |
| 2514 | | |
| 2515 | | Fit map W20_J4021_005_volume_map_sharp_bprime_local.mrc in map |
| 2516 | | W20_J3623_consensus_structure_sharp.mrc using 137893 points |
| 2517 | | correlation = 0.8085, correlation about mean = 0.2734, overlap = 2.896e+04 |
| 2518 | | steps = 48, shift = 0.598, angle = 0.487 degrees |
| 2519 | | |
| 2520 | | Position of W20_J4021_005_volume_map_sharp_bprime_local.mrc (#14) relative to |
| 2521 | | W20_J3623_consensus_structure_sharp.mrc (#1) coordinates: |
| 2522 | | Matrix rotation and translation |
| 2523 | | 0.99997911 0.00638470 0.00100265 -1.04735728 |
| 2524 | | -0.00637907 0.99996444 -0.00551663 1.34954856 |
| 2525 | | -0.00103783 0.00551011 0.99998428 -0.33308547 |
| 2526 | | Axis 0.64900636 0.12009752 -0.75124386 |
| 2527 | | Axis point 215.82672874 137.56284372 -0.00000000 |
| 2528 | | Rotation angle (degrees) 0.48673885 |
| 2529 | | Shift along axis -0.26743568 |
| 2530 | | |
| 2531 | | |
| 2532 | | > hide #!14 models |
| 2533 | | |
| 2534 | | > hide #!13 models |
| 2535 | | |
| 2536 | | > show #!15 models |
| 2537 | | |
| 2538 | | > show #!16 models |
| 2539 | | |
| 2540 | | > fitmap #15 inMap #1 |
| 2541 | | |
| 2542 | | Fit map W20_J4022_005_volume_map_bprime_local.mrc in map |
| 2543 | | W20_J3623_consensus_structure_sharp.mrc using 138200 points |
| 2544 | | correlation = 0.8381, correlation about mean = 0.222, overlap = 1.906e+04 |
| 2545 | | steps = 40, shift = 0.303, angle = 0.34 degrees |
| 2546 | | |
| 2547 | | Position of W20_J4022_005_volume_map_bprime_local.mrc (#15) relative to |
| 2548 | | W20_J3623_consensus_structure_sharp.mrc (#1) coordinates: |
| 2549 | | Matrix rotation and translation |
| 2550 | | 0.99998447 0.00240641 0.00502780 -1.28358869 |
| 2551 | | -0.00241668 0.99999501 0.00203622 -0.22148190 |
| 2552 | | -0.00502288 -0.00204834 0.99998529 1.05482120 |
| 2553 | | Axis -0.34402829 0.84653419 -0.40623194 |
| 2554 | | Axis point 195.11366625 0.00000000 267.83446555 |
| 2555 | | Rotation angle (degrees) 0.34013109 |
| 2556 | | Shift along axis -0.17440325 |
| 2557 | | |
| 2558 | | |
| 2559 | | > fitmap #16 inMap #1 |
| 2560 | | |
| 2561 | | Fit map W20_J4022_005_volume_map_sharp_bprime_local.mrc in map |
| 2562 | | W20_J3623_consensus_structure_sharp.mrc using 138084 points |
| 2563 | | correlation = 0.8046, correlation about mean = 0.274, overlap = 2.955e+04 |
| 2564 | | steps = 48, shift = 0.324, angle = 0.293 degrees |
| 2565 | | |
| 2566 | | Position of W20_J4022_005_volume_map_sharp_bprime_local.mrc (#16) relative to |
| 2567 | | W20_J3623_consensus_structure_sharp.mrc (#1) coordinates: |
| 2568 | | Matrix rotation and translation |
| 2569 | | 0.99999153 0.00141184 0.00386537 -0.96250876 |
| 2570 | | -0.00142356 0.99999440 0.00302979 -0.53091994 |
| 2571 | | -0.00386107 -0.00303526 0.99998794 0.99298679 |
| 2572 | | Axis -0.59323978 0.75574456 -0.27733863 |
| 2573 | | Axis point 248.76134938 0.00000000 265.76556629 |
| 2574 | | Rotation angle (degrees) 0.29288616 |
| 2575 | | Shift along axis -0.10563498 |
| 2576 | | |
| 2577 | | |
| 2578 | | > hide #!16 models |
| 2579 | | |
| 2580 | | > hide #!15 models |
| 2581 | | |
| 2582 | | > show #!17 models |
| 2583 | | |
| 2584 | | > fitmap #17 inMap #1 |
| 2585 | | |
| 2586 | | Fit map W20_J4023_003_volume_map_bprime_local.mrc in map |
| 2587 | | W20_J3623_consensus_structure_sharp.mrc using 138098 points |
| 2588 | | correlation = 0.8373, correlation about mean = 0.2151, overlap = 1.874e+04 |
| 2589 | | steps = 68, shift = 0.363, angle = 0.333 degrees |
| 2590 | | |
| 2591 | | Position of W20_J4023_003_volume_map_bprime_local.mrc (#17) relative to |
| 2592 | | W20_J3623_consensus_structure_sharp.mrc (#1) coordinates: |
| 2593 | | Matrix rotation and translation |
| 2594 | | 0.99998345 0.00557936 0.00140325 -1.18658067 |
| 2595 | | -0.00557823 0.99998411 -0.00081217 0.60425107 |
| 2596 | | -0.00140776 0.00080432 0.99999869 0.20586820 |
| 2597 | | Axis 0.13912185 0.24192785 -0.96026873 |
| 2598 | | Axis point 117.12498270 207.62002695 0.00000000 |
| 2599 | | Rotation angle (degrees) 0.33286852 |
| 2600 | | Shift along axis -0.21658293 |
| 2601 | | |
| 2602 | | |
| 2603 | | > show #!18 models |
| 2604 | | |
| 2605 | | > fitmap #18 inMap #1 |
| 2606 | | |
| 2607 | | Fit map W20_J4023_003_volume_map_sharp_bprime_local.mrc in map |
| 2608 | | W20_J3623_consensus_structure_sharp.mrc using 138171 points |
| 2609 | | correlation = 0.7947, correlation about mean = 0.312, overlap = 3.526e+04 |
| 2610 | | steps = 68, shift = 0.461, angle = 0.264 degrees |
| 2611 | | |
| 2612 | | Position of W20_J4023_003_volume_map_sharp_bprime_local.mrc (#18) relative to |
| 2613 | | W20_J3623_consensus_structure_sharp.mrc (#1) coordinates: |
| 2614 | | Matrix rotation and translation |
| 2615 | | 0.99999048 0.00393326 0.00188760 -1.06407059 |
| 2616 | | -0.00393046 0.99999117 -0.00148458 0.41525484 |
| 2617 | | -0.00189342 0.00147715 0.99999712 0.25585356 |
| 2618 | | Axis 0.32142171 0.41033635 -0.85341207 |
| 2619 | | Axis point 145.82615217 239.01637261 0.00000000 |
| 2620 | | Rotation angle (degrees) 0.26397543 |
| 2621 | | Shift along axis -0.38996975 |
| 2622 | | |
| 2623 | | |
| 2624 | | > hide #!18 models |
| 2625 | | |
| 2626 | | > hide #!17 models |
| 2627 | | |
| 2628 | | > show #!19 models |
| 2629 | | |
| 2630 | | > fitmap #19 inMap #1 |
| 2631 | | |
| 2632 | | Fit map W20_J4024_005_volume_map_bprime_local.mrc in map |
| 2633 | | W20_J3623_consensus_structure_sharp.mrc using 138193 points |
| 2634 | | correlation = 0.8392, correlation about mean = 0.2773, overlap = 1.921e+04 |
| 2635 | | steps = 76, shift = 0.289, angle = 0.259 degrees |
| 2636 | | |
| 2637 | | Position of W20_J4024_005_volume_map_bprime_local.mrc (#19) relative to |
| 2638 | | W20_J3623_consensus_structure_sharp.mrc (#1) coordinates: |
| 2639 | | Matrix rotation and translation |
| 2640 | | 0.99999040 0.00368324 0.00237314 -0.77269801 |
| 2641 | | -0.00368586 0.99999260 0.00110036 0.16957827 |
| 2642 | | -0.00236907 -0.00110910 0.99999658 0.39066327 |
| 2643 | | Axis -0.24448082 0.52473318 -0.81540433 |
| 2644 | | Axis point 51.36886426 212.61995395 0.00000000 |
| 2645 | | Rotation angle (degrees) 0.25890214 |
| 2646 | | Shift along axis -0.04065533 |
| 2647 | | |
| 2648 | | |
| 2649 | | > fitmap #20 inMap #1 |
| 2650 | | |
| 2651 | | Fit map W20_J4024_005_volume_map_sharp_bprime_local.mrc in map |
| 2652 | | W20_J3623_consensus_structure_sharp.mrc using 138123 points |
| 2653 | | correlation = 0.8039, correlation about mean = 0.2975, overlap = 2.959e+04 |
| 2654 | | steps = 48, shift = 0.294, angle = 0.249 degrees |
| 2655 | | |
| 2656 | | Position of W20_J4024_005_volume_map_sharp_bprime_local.mrc (#20) relative to |
| 2657 | | W20_J3623_consensus_structure_sharp.mrc (#1) coordinates: |
| 2658 | | Matrix rotation and translation |
| 2659 | | 0.99999177 0.00343445 0.00216100 -0.68440650 |
| 2660 | | -0.00343784 0.99999286 0.00156920 0.06668376 |
| 2661 | | -0.00215559 -0.00157662 0.99999643 0.45265088 |
| 2662 | | Axis -0.36142806 0.49593828 -0.78956633 |
| 2663 | | Axis point 30.06869516 207.44842694 0.00000000 |
| 2664 | | Rotation angle (degrees) 0.24934854 |
| 2665 | | Shift along axis -0.07696315 |
| 2666 | | |
| 2667 | | |
| 2668 | | > hide #!19 models |
| 2669 | | |
| 2670 | | > show #!20 models |
| 2671 | | |
| 2672 | | > hide #!20 models |
| 2673 | | |
| 2674 | | > show #!21 models |
| 2675 | | |
| 2676 | | > fitmap #21 inMap #1 |
| 2677 | | |
| 2678 | | Fit map W20_J4025_004_volume_map_bprime_local.mrc in map |
| 2679 | | W20_J3623_consensus_structure_sharp.mrc using 138084 points |
| 2680 | | correlation = 0.8289, correlation about mean = 0.1891, overlap = 1.832e+04 |
| 2681 | | steps = 48, shift = 0.728, angle = 0.413 degrees |
| 2682 | | |
| 2683 | | Position of W20_J4025_004_volume_map_bprime_local.mrc (#21) relative to |
| 2684 | | W20_J3623_consensus_structure_sharp.mrc (#1) coordinates: |
| 2685 | | Matrix rotation and translation |
| 2686 | | 0.99998460 0.00544119 0.00108928 -0.83012202 |
| 2687 | | -0.00543611 0.99997458 -0.00461454 1.00696868 |
| 2688 | | -0.00111436 0.00460855 0.99998876 -0.07337997 |
| 2689 | | Axis 0.63914215 0.15270809 -0.75377553 |
| 2690 | | Axis point 193.72853355 115.34838902 0.00000000 |
| 2691 | | Rotation angle (degrees) 0.41340458 |
| 2692 | | Shift along axis -0.32148169 |
| 2693 | | |
| 2694 | | |
| 2695 | | > fitmap #22 inMap #1 |
| 2696 | | |
| 2697 | | Fit map W20_J4025_004_volume_map_sharp_bprime_local.mrc in map |
| 2698 | | W20_J3623_consensus_structure_sharp.mrc using 137654 points |
| 2699 | | correlation = 0.7511, correlation about mean = 0.2821, overlap = 3.85e+04 |
| 2700 | | steps = 52, shift = 0.819, angle = 0.498 degrees |
| 2701 | | |
| 2702 | | Position of W20_J4025_004_volume_map_sharp_bprime_local.mrc (#22) relative to |
| 2703 | | W20_J3623_consensus_structure_sharp.mrc (#1) coordinates: |
| 2704 | | Matrix rotation and translation |
| 2705 | | 0.99998155 0.00559478 0.00236633 -1.03081576 |
| 2706 | | -0.00557996 0.99996507 -0.00622345 1.34930492 |
| 2707 | | -0.00240107 0.00621013 0.99997783 -0.00730733 |
| 2708 | | Axis 0.71523902 0.27424352 -0.64282473 |
| 2709 | | Axis point 0.00000000 39.53456477 232.56399021 |
| 2710 | | Rotation angle (degrees) 0.49801542 |
| 2711 | | Shift along axis -0.36254420 |
| 2712 | | |
| 2713 | | |
| 2714 | | > show #!22 models |
| 2715 | | |
| 2716 | | > hide #!22 models |
| 2717 | | |
| 2718 | | > hide #!21 models |
| 2719 | | |
| 2720 | | > show #!23 models |
| 2721 | | |
| 2722 | | > show #!24 models |
| 2723 | | |
| 2724 | | > fitmap #23 inMap #1 |
| 2725 | | |
| 2726 | | Fit map W20_J4026_004_volume_map_gammazeta_local.mrc in map |
| 2727 | | W20_J3623_consensus_structure_sharp.mrc using 138172 points |
| 2728 | | correlation = 0.8607, correlation about mean = 0.3259, overlap = 2.201e+04 |
| 2729 | | steps = 52, shift = 0.541, angle = 0.494 degrees |
| 2730 | | |
| 2731 | | Position of W20_J4026_004_volume_map_gammazeta_local.mrc (#23) relative to |
| 2732 | | W20_J3623_consensus_structure_sharp.mrc (#1) coordinates: |
| 2733 | | Matrix rotation and translation |
| 2734 | | 0.99997866 -0.00017168 -0.00653102 0.57575571 |
| 2735 | | 0.00020836 0.99998421 0.00561565 -0.37594588 |
| 2736 | | 0.00652995 -0.00561689 0.99996290 0.02009032 |
| 2737 | | Axis -0.65188430 -0.75799762 0.02205601 |
| 2738 | | Axis point -2.93047519 0.00000000 79.19172412 |
| 2739 | | Rotation angle (degrees) 0.49363480 |
| 2740 | | Shift along axis -0.08991691 |
| 2741 | | |
| 2742 | | |
| 2743 | | > fitmap #24 inMap #1 |
| 2744 | | |
| 2745 | | Fit map W20_J4026_004_volume_map_sharp_gammazeta_local.mrc in map |
| 2746 | | W20_J3623_consensus_structure_sharp.mrc using 138033 points |
| 2747 | | correlation = 0.853, correlation about mean = 0.3795, overlap = 2.779e+04 |
| 2748 | | steps = 68, shift = 0.477, angle = 0.571 degrees |
| 2749 | | |
| 2750 | | Position of W20_J4026_004_volume_map_sharp_gammazeta_local.mrc (#24) relative |
| 2751 | | to W20_J3623_consensus_structure_sharp.mrc (#1) coordinates: |
| 2752 | | Matrix rotation and translation |
| 2753 | | 0.99996930 -0.00126227 -0.00773336 0.96231167 |
| 2754 | | 0.00130980 0.99998026 0.00614469 -0.62594677 |
| 2755 | | 0.00772545 -0.00615463 0.99995122 -0.10724743 |
| 2756 | | Axis -0.61739139 -0.77598860 0.12911066 |
| 2757 | | Axis point 12.56321001 0.00000000 114.62740380 |
| 2758 | | Rotation angle (degrees) 0.57071652 |
| 2759 | | Shift along axis -0.12224217 |
| 2760 | | |
| 2761 | | |
| 2762 | | > hide #!24 models |
| 2763 | | |
| 2764 | | > hide #!23 models |
| 2765 | | |
| 2766 | | > show #!25 models |
| 2767 | | |
| 2768 | | > show #!26 models |
| 2769 | | |
| 2770 | | > fitmap #25 inMap #1 |
| 2771 | | |
| 2772 | | Fit map W20_J4027_005_volume_map_gammazeta_local.mrc in map |
| 2773 | | W20_J3623_consensus_structure_sharp.mrc using 138160 points |
| 2774 | | correlation = 0.8845, correlation about mean = 0.4248, overlap = 2.208e+04 |
| 2775 | | steps = 48, shift = 0.937, angle = 0.528 degrees |
| 2776 | | |
| 2777 | | Position of W20_J4027_005_volume_map_gammazeta_local.mrc (#25) relative to |
| 2778 | | W20_J3623_consensus_structure_sharp.mrc (#1) coordinates: |
| 2779 | | Matrix rotation and translation |
| 2780 | | 0.99995803 -0.00131908 0.00906657 -1.56861951 |
| 2781 | | 0.00131039 0.99999868 0.00096483 0.05951128 |
| 2782 | | -0.00906783 -0.00095291 0.99995843 2.12791102 |
| 2783 | | Axis -0.10408825 0.98427482 0.14271899 |
| 2784 | | Axis point 225.62409153 0.00000000 168.02231673 |
| 2785 | | Rotation angle (degrees) 0.52781987 |
| 2786 | | Shift along axis 0.52554363 |
| 2787 | | |
| 2788 | | |
| 2789 | | > fitmap #26 inMap #1 |
| 2790 | | |
| 2791 | | Fit map W20_J4027_005_volume_map_sharp_gammazeta_local.mrc in map |
| 2792 | | W20_J3623_consensus_structure_sharp.mrc using 138211 points |
| 2793 | | correlation = 0.8774, correlation about mean = 0.4648, overlap = 2.896e+04 |
| 2794 | | steps = 48, shift = 0.956, angle = 0.621 degrees |
| 2795 | | |
| 2796 | | Position of W20_J4027_005_volume_map_sharp_gammazeta_local.mrc (#26) relative |
| 2797 | | to W20_J3623_consensus_structure_sharp.mrc (#1) coordinates: |
| 2798 | | Matrix rotation and translation |
| 2799 | | 0.99994135 -0.00098967 0.01078522 -1.85651387 |
| 2800 | | 0.00098601 0.99999945 0.00034465 0.18754839 |
| 2801 | | -0.01078555 -0.00033399 0.99994178 2.30481893 |
| 2802 | | Axis -0.03131444 0.99534338 0.09116443 |
| 2803 | | Axis point 208.92157622 0.00000000 171.95034602 |
| 2804 | | Rotation angle (degrees) 0.62086019 |
| 2805 | | Shift along axis 0.45492826 |
| 2806 | | |
| 2807 | | |
| 2808 | | > hide #!26 models |
| 2809 | | |
| 2810 | | > hide #!25 models |
| 2811 | | |
| 2812 | | > show #!27 models |
| 2813 | | |
| 2814 | | > fitmap #27 inMap #1 |
| 2815 | | |
| 2816 | | Fit map W20_J4028_004_volume_map_gammazeta_local.mrc in map |
| 2817 | | W20_J3623_consensus_structure_sharp.mrc using 138218 points |
| 2818 | | correlation = 0.8752, correlation about mean = 0.4062, overlap = 2.23e+04 |
| 2819 | | steps = 44, shift = 0.478, angle = 0.403 degrees |
| 2820 | | |
| 2821 | | Position of W20_J4028_004_volume_map_gammazeta_local.mrc (#27) relative to |
| 2822 | | W20_J3623_consensus_structure_sharp.mrc (#1) coordinates: |
| 2823 | | Matrix rotation and translation |
| 2824 | | 0.99997775 0.00042654 -0.00665697 0.77827945 |
| 2825 | | -0.00041158 0.99999739 0.00224763 0.14049133 |
| 2826 | | 0.00665791 -0.00224484 0.99997532 -0.37918036 |
| 2827 | | Axis -0.31912872 -0.94583944 -0.05953667 |
| 2828 | | Axis point 60.52816960 0.00000000 99.51494449 |
| 2829 | | Rotation angle (degrees) 0.40328886 |
| 2830 | | Shift along axis -0.35867843 |
| 2831 | | |
| 2832 | | |
| 2833 | | > fitmap #28 inMap #1 |
| 2834 | | |
| 2835 | | Fit map W20_J4028_004_volume_map_sharp_gammazeta_local.mrc in map |
| 2836 | | W20_J3623_consensus_structure_sharp.mrc using 137995 points |
| 2837 | | correlation = 0.8679, correlation about mean = 0.4447, overlap = 2.897e+04 |
| 2838 | | steps = 48, shift = 0.432, angle = 0.487 degrees |
| 2839 | | |
| 2840 | | Position of W20_J4028_004_volume_map_sharp_gammazeta_local.mrc (#28) relative |
| 2841 | | to W20_J3623_consensus_structure_sharp.mrc (#1) coordinates: |
| 2842 | | Matrix rotation and translation |
| 2843 | | 0.99996809 -0.00001305 -0.00798826 1.06130917 |
| 2844 | | 0.00003628 0.99999577 0.00290797 -0.02300922 |
| 2845 | | 0.00798819 -0.00290817 0.99996387 -0.53329988 |
| 2846 | | Axis -0.34208068 -0.93966610 0.00290175 |
| 2847 | | Axis point 67.16980128 0.00000000 117.90291440 |
| 2848 | | Rotation angle (degrees) 0.48708480 |
| 2849 | | Shift along axis -0.34297988 |
| 2850 | | |
| 2851 | | |
| 2852 | | > show #!28 models |
| 2853 | | |
| 2854 | | > hide #!28 models |
| 2855 | | |
| 2856 | | > show #!28 models |
| 2857 | | |
| 2858 | | > hide #!28 models |
| 2859 | | |
| 2860 | | > hide #!27 models |
| 2861 | | |
| 2862 | | > save "/Users/becca/Desktop/Postdoc/COPI notebooks and |
| 2863 | | > resources/Processing/W20_MM_cyto_COPI/20250701_W20_COPI_cyto_alignments.cxs" |
| 2864 | | |
| 2865 | | > open "/Users/becca/Desktop/Postdoc/COPI notebooks and |
| 2866 | | > resources/Processing/W20_MM_cyto_COPI/relionMB/W20_J140_run_body001.mrc" |
| 2867 | | > "/Users/becca/Desktop/Postdoc/COPI notebooks and |
| 2868 | | > resources/Processing/W20_MM_cyto_COPI/relionMB/W20_J140_run_body002.mrc" |
| 2869 | | > "/Users/becca/Desktop/Postdoc/COPI notebooks and |
| 2870 | | > resources/Processing/W20_MM_cyto_COPI/relionMB/W20_J140_run_body003.mrc" |
| 2871 | | > "/Users/becca/Desktop/Postdoc/COPI notebooks and |
| 2872 | | > resources/Processing/W20_MM_cyto_COPI/relionMB/W20_J140_run_body004.mrc" |
| 2873 | | |
| 2874 | | Opened W20_J140_run_body001.mrc as #29.1, grid size 240,240,240, pixel 1.2, |
| 2875 | | shown at level 0.00608, step 1, values float32 |
| 2876 | | Opened W20_J140_run_body002.mrc as #29.2, grid size 240,240,240, pixel 1.2, |
| 2877 | | shown at level 0.00134, step 1, values float32 |
| 2878 | | Opened W20_J140_run_body003.mrc as #29.3, grid size 240,240,240, pixel 1.2, |
| 2879 | | shown at level 0.000955, step 1, values float32 |
| 2880 | | Opened W20_J140_run_body004.mrc as #29.4, grid size 240,240,240, pixel 1.2, |
| 2881 | | shown at level 0.000493, step 1, values float32 |
| 2882 | | |
| 2883 | | > select add #29 |
| 2884 | | |
| 2885 | | 9 models selected |
| 2886 | | |
| 2887 | | > view matrix models |
| 2888 | | > #29,-0.47366,-0.87946,-0.046912,333.91,-0.83829,0.43388,0.33018,153.37,-0.27003,0.19572,-0.94275,297.34 |
| 2889 | | |
| 2890 | | > ui mousemode right "translate selected models" |
| 2891 | | |
| 2892 | | > view matrix models |
| 2893 | | > #29,-0.47366,-0.87946,-0.046912,340.41,-0.83829,0.43388,0.33018,139.03,-0.27003,0.19572,-0.94275,298.38 |
| 2894 | | |
| 2895 | | > fitmap #29 inMap #1 |
| 2896 | | |
| 2897 | | Multiple maps for #29 |
| 2898 | | |
| 2899 | | > fitmap #29.1 inMap #1 |
| 2900 | | |
| 2901 | | Fit map W20_J140_run_body001.mrc in map |
| 2902 | | W20_J3623_consensus_structure_sharp.mrc using 138225 points |
| 2903 | | correlation = 0.9233, correlation about mean = 0.7294, overlap = 874.3 |
| 2904 | | steps = 156, shift = 14.4, angle = 22 degrees |
| 2905 | | |
| 2906 | | Position of W20_J140_run_body001.mrc (#29.1) relative to |
| 2907 | | W20_J3623_consensus_structure_sharp.mrc (#1) coordinates: |
| 2908 | | Matrix rotation and translation |
| 2909 | | 0.95200729 0.18684598 -0.24242669 1.82192845 |
| 2910 | | -0.12799492 0.96249869 0.23919359 -9.97975402 |
| 2911 | | 0.27802773 -0.19668465 0.94022111 9.66133125 |
| 2912 | | Axis -0.58247333 -0.69549428 -0.42072857 |
| 2913 | | Axis point -33.51580829 0.00000000 18.51090823 |
| 2914 | | Rotation angle (degrees) 21.97250066 |
| 2915 | | Shift along axis 1.81483897 |
| 2916 | | |
| 2917 | | |
| 2918 | | > fitmap #29.2 inMap #1 |
| 2919 | | |
| 2920 | | Fit map W20_J140_run_body002.mrc in map |
| 2921 | | W20_J3623_consensus_structure_sharp.mrc using 138046 points |
| 2922 | | correlation = 0.8263, correlation about mean = 0.6371, overlap = 468.8 |
| 2923 | | steps = 280, shift = 31, angle = 21.8 degrees |
| 2924 | | |
| 2925 | | Position of W20_J140_run_body002.mrc (#29.2) relative to |
| 2926 | | W20_J3623_consensus_structure_sharp.mrc (#1) coordinates: |
| 2927 | | Matrix rotation and translation |
| 2928 | | 0.95172436 0.17711878 -0.25069840 4.25540621 |
| 2929 | | -0.11810903 0.96515525 0.23350719 -10.43311409 |
| 2930 | | 0.28332138 -0.19262473 0.93948108 8.13777130 |
| 2931 | | Axis -0.57255681 -0.71751644 -0.39667222 |
| 2932 | | Axis point -25.70139502 0.00000000 26.08327898 |
| 2933 | | Rotation angle (degrees) 21.84708246 |
| 2934 | | Shift along axis 1.82144130 |
| 2935 | | |
| 2936 | | |
| 2937 | | > fitmap #29.3 inMap #1 |
| 2938 | | |
| 2939 | | Fit map W20_J140_run_body003.mrc in map |
| 2940 | | W20_J3623_consensus_structure_sharp.mrc using 137660 points |
| 2941 | | correlation = 0.5389, correlation about mean = 0.1615, overlap = 221.3 |
| 2942 | | steps = 180, shift = 25.4, angle = 11.9 degrees |
| 2943 | | |
| 2944 | | Position of W20_J140_run_body003.mrc (#29.3) relative to |
| 2945 | | W20_J3623_consensus_structure_sharp.mrc (#1) coordinates: |
| 2946 | | Matrix rotation and translation |
| 2947 | | 0.98378563 0.15518997 -0.08989941 -25.87512376 |
| 2948 | | -0.14501363 0.98324752 0.11043264 11.64818978 |
| 2949 | | 0.10553141 -0.09560540 0.98980944 10.88183562 |
| 2950 | | Axis -0.49859202 -0.47292359 -0.72646353 |
| 2951 | | Axis point 58.49450968 174.49109696 0.00000000 |
| 2952 | | Rotation angle (degrees) 11.92434111 |
| 2953 | | Shift along axis -0.51283023 |
| 2954 | | |
| 2955 | | |
| 2956 | | > fitmap #29.4 inMap #1 |
| 2957 | | |
| 2958 | | Fit map W20_J140_run_body004.mrc in map |
| 2959 | | W20_J3623_consensus_structure_sharp.mrc using 137970 points |
| 2960 | | correlation = 0.7171, correlation about mean = 0.4553, overlap = 200.6 |
| 2961 | | steps = 192, shift = 16.7, angle = 21.7 degrees |
| 2962 | | |
| 2963 | | Position of W20_J140_run_body004.mrc (#29.4) relative to |
| 2964 | | W20_J3623_consensus_structure_sharp.mrc (#1) coordinates: |
| 2965 | | Matrix rotation and translation |
| 2966 | | 0.95301433 0.19868762 -0.22866331 -0.93326951 |
| 2967 | | -0.14395018 0.96121397 0.23525740 -8.31020320 |
| 2968 | | 0.26653710 -0.19128756 0.94465181 8.81821407 |
| 2969 | | Axis -0.57801628 -0.67105211 -0.46431266 |
| 2970 | | Axis point -34.90313676 0.00000000 8.20072337 |
| 2971 | | Rotation angle (degrees) 21.65230186 |
| 2972 | | Shift along axis 2.02161595 |
| 2973 | | |
| 2974 | | |
| 2975 | | > select subtract #29 |
| 2976 | | |
| 2977 | | Nothing selected |
| 2978 | | |
| 2979 | | > select add #29 |
| 2980 | | |
| 2981 | | 9 models selected |
| 2982 | | |
| 2983 | | > select add #29.1 |
| 2984 | | |
| 2985 | | 9 models selected |
| 2986 | | |
| 2987 | | > select subtract #29.1 |
| 2988 | | |
| 2989 | | 7 models selected |
| 2990 | | |
| 2991 | | > select add #29.1 |
| 2992 | | |
| 2993 | | 9 models selected |
| 2994 | | |
| 2995 | | > select subtract #29.1 |
| 2996 | | |
| 2997 | | 7 models selected |
| 2998 | | |
| 2999 | | > color #29 darkgrey models |
| 3000 | | |
| 3001 | | > color #29 #a9a9a993 models |
| 3002 | | |
| 3003 | | > color #29 #a9a9a994 models |
| 3004 | | |
| 3005 | | > select add #29 |
| 3006 | | |
| 3007 | | 9 models selected |
| 3008 | | |
| 3009 | | > select subtract #29 |
| 3010 | | |
| 3011 | | Nothing selected |
| 3012 | | |
| 3013 | | > hide #!29 models |
| 3014 | | |
| 3015 | | > rename #29 W20_J140_MB |
| 3016 | | |
| 3017 | | > open "/Users/becca/Desktop/Postdoc/COPI notebooks and |
| 3018 | | > resources/Processing/W20_MM_cyto_COPI/relionMB/W20_J145_run_ct8_body001.mrc" |
| 3019 | | > "/Users/becca/Desktop/Postdoc/COPI notebooks and |
| 3020 | | > resources/Processing/W20_MM_cyto_COPI/relionMB/W20_J145_run_ct8_body002.mrc" |
| 3021 | | > "/Users/becca/Desktop/Postdoc/COPI notebooks and |
| 3022 | | > resources/Processing/W20_MM_cyto_COPI/relionMB/W20_J145_run_ct8_body003.mrc" |
| 3023 | | > "/Users/becca/Desktop/Postdoc/COPI notebooks and |
| 3024 | | > resources/Processing/W20_MM_cyto_COPI/relionMB/W20_J145_run_ct8_body004.mrc" |
| 3025 | | |
| 3026 | | Opened W20_J145_run_ct8_body001.mrc as #30.1, grid size 240,240,240, pixel |
| 3027 | | 1.2, shown at level 0.00616, step 1, values float32 |
| 3028 | | Opened W20_J145_run_ct8_body002.mrc as #30.2, grid size 240,240,240, pixel |
| 3029 | | 1.2, shown at level 0.00152, step 1, values float32 |
| 3030 | | Opened W20_J145_run_ct8_body003.mrc as #30.3, grid size 240,240,240, pixel |
| 3031 | | 1.2, shown at level 0.000716, step 1, values float32 |
| 3032 | | Opened W20_J145_run_ct8_body004.mrc as #30.4, grid size 240,240,240, pixel |
| 3033 | | 1.2, shown at level 0.000812, step 1, values float32 |
| 3034 | | |
| 3035 | | > select add #30 |
| 3036 | | |
| 3037 | | 9 models selected |
| 3038 | | |
| 3039 | | > ui mousemode right "rotate selected models" |
| 3040 | | |
| 3041 | | > view matrix models |
| 3042 | | > #30,-0.35278,-0.9274,-0.12442,348.28,-0.78005,0.21805,0.5865,134.81,-0.51679,0.30396,-0.80034,301.49 |
| 3043 | | |
| 3044 | | > ui mousemode right "translate selected models" |
| 3045 | | |
| 3046 | | > view matrix models |
| 3047 | | > #30,-0.35278,-0.9274,-0.12442,340.27,-0.78005,0.21805,0.5865,136.72,-0.51679,0.30396,-0.80034,288.45 |
| 3048 | | |
| 3049 | | > fitmap #30.4 inMap #1 |
| 3050 | | |
| 3051 | | Fit map W20_J145_run_ct8_body004.mrc in map |
| 3052 | | W20_J3623_consensus_structure_sharp.mrc using 137951 points |
| 3053 | | correlation = 0.7282, correlation about mean = 0.4288, overlap = 228.8 |
| 3054 | | steps = 112, shift = 11.5, angle = 9.2 degrees |
| 3055 | | |
| 3056 | | Position of W20_J145_run_ct8_body004.mrc (#30.4) relative to |
| 3057 | | W20_J3623_consensus_structure_sharp.mrc (#1) coordinates: |
| 3058 | | Matrix rotation and translation |
| 3059 | | 0.98788522 -0.14632868 -0.05167889 26.91126566 |
| 3060 | | 0.14822194 0.98833778 0.03490982 -21.95721425 |
| 3061 | | 0.04596789 -0.04214685 0.99805340 -1.50223115 |
| 3062 | | Axis -0.24099906 -0.30539579 0.92122357 |
| 3063 | | Axis point 163.78780037 168.43265871 0.00000000 |
| 3064 | | Rotation angle (degrees) 9.19930630 |
| 3065 | | Shift along axis -1.16383962 |
| 3066 | | |
| 3067 | | |
| 3068 | | > fitmap #30.3 inMap #1 |
| 3069 | | |
| 3070 | | Fit map W20_J145_run_ct8_body003.mrc in map |
| 3071 | | W20_J3623_consensus_structure_sharp.mrc using 137543 points |
| 3072 | | correlation = 0.6493, correlation about mean = 0.3323, overlap = 237.6 |
| 3073 | | steps = 116, shift = 8.58, angle = 1.99 degrees |
| 3074 | | |
| 3075 | | Position of W20_J145_run_ct8_body003.mrc (#30.3) relative to |
| 3076 | | W20_J3623_consensus_structure_sharp.mrc (#1) coordinates: |
| 3077 | | Matrix rotation and translation |
| 3078 | | 0.99943608 -0.00513122 -0.03318412 -0.25293108 |
| 3079 | | 0.00484442 0.99995027 -0.00871730 -4.33467984 |
| 3080 | | 0.03322720 0.00855163 0.99941124 -5.67738477 |
| 3081 | | Axis 0.24904308 -0.95774784 0.14386320 |
| 3082 | | Axis point 184.40566041 0.00000000 -35.31005024 |
| 3083 | | Rotation angle (degrees) 1.98687515 |
| 3084 | | Shift along axis 3.27177278 |
| 3085 | | |
| 3086 | | |
| 3087 | | > fitmap #30.2 inMap #1 |
| 3088 | | |
| 3089 | | Fit map W20_J145_run_ct8_body002.mrc in map |
| 3090 | | W20_J3623_consensus_structure_sharp.mrc using 137963 points |
| 3091 | | correlation = 0.8212, correlation about mean = 0.6198, overlap = 468.2 |
| 3092 | | steps = 92, shift = 7.64, angle = 3.06 degrees |
| 3093 | | |
| 3094 | | Position of W20_J145_run_ct8_body002.mrc (#30.2) relative to |
| 3095 | | W20_J3623_consensus_structure_sharp.mrc (#1) coordinates: |
| 3096 | | Matrix rotation and translation |
| 3097 | | 0.99870570 0.02334084 -0.04519002 -1.99430024 |
| 3098 | | -0.02260011 0.99960285 0.01683358 -2.36740438 |
| 3099 | | 0.04556498 -0.01579049 0.99883657 -3.32045770 |
| 3100 | | Axis -0.30540022 -0.84957484 -0.43006196 |
| 3101 | | Axis point 34.20968149 0.00000000 -17.75195541 |
| 3102 | | Rotation angle (degrees) 3.06173952 |
| 3103 | | Shift along axis 4.04834949 |
| 3104 | | |
| 3105 | | |
| 3106 | | > fitmap #30.1 inMap #1 |
| 3107 | | |
| 3108 | | Fit map W20_J145_run_ct8_body001.mrc in map |
| 3109 | | W20_J3623_consensus_structure_sharp.mrc using 138203 points |
| 3110 | | correlation = 0.9153, correlation about mean = 0.7173, overlap = 857.4 |
| 3111 | | steps = 84, shift = 6.27, angle = 2.89 degrees |
| 3112 | | |
| 3113 | | Position of W20_J145_run_ct8_body001.mrc (#30.1) relative to |
| 3114 | | W20_J3623_consensus_structure_sharp.mrc (#1) coordinates: |
| 3115 | | Matrix rotation and translation |
| 3116 | | 0.99917915 0.02581546 -0.03121838 -4.27539402 |
| 3117 | | -0.02486602 0.99922761 0.03042809 -4.63326697 |
| 3118 | | 0.03197979 -0.02962683 0.99904932 0.16478454 |
| 3119 | | Axis -0.59553173 -0.62670160 -0.50258041 |
| 3120 | | Axis point -90.45500707 0.00000000 -32.19034476 |
| 3121 | | Rotation angle (degrees) 2.89015105 |
| 3122 | | Shift along axis 5.36699111 |
| 3123 | | |
| 3124 | | |
| 3125 | | > color #30 darkgrey models |
| 3126 | | |
| 3127 | | > color #30 #a9a9a980 models |
| 3128 | | |
| 3129 | | > select subtract #30 |
| 3130 | | |
| 3131 | | Nothing selected |
| 3132 | | |
| 3133 | | > hide #!30 models |
| 3134 | | |
| 3135 | | > rename #30 W20_J145_MB |
| 3136 | | |
| 3137 | | > open "/Users/becca/Desktop/Postdoc/COPI notebooks and |
| 3138 | | > resources/Processing/W20_MM_cyto_COPI/relionMB/W20_J150_run_body001.mrc" |
| 3139 | | > "/Users/becca/Desktop/Postdoc/COPI notebooks and |
| 3140 | | > resources/Processing/W20_MM_cyto_COPI/relionMB/W20_J150_run_body002.mrc" |
| 3141 | | > "/Users/becca/Desktop/Postdoc/COPI notebooks and |
| 3142 | | > resources/Processing/W20_MM_cyto_COPI/relionMB/W20_J150_run_body003.mrc" |
| 3143 | | > "/Users/becca/Desktop/Postdoc/COPI notebooks and |
| 3144 | | > resources/Processing/W20_MM_cyto_COPI/relionMB/W20_J150_run_body004.mrc" |
| 3145 | | |
| 3146 | | Opened W20_J150_run_body001.mrc as #31.1, grid size 240,240,240, pixel 1.2, |
| 3147 | | shown at level 0.0066, step 1, values float32 |
| 3148 | | Opened W20_J150_run_body002.mrc as #31.2, grid size 240,240,240, pixel 1.2, |
| 3149 | | shown at level 0.0013, step 1, values float32 |
| 3150 | | Opened W20_J150_run_body003.mrc as #31.3, grid size 240,240,240, pixel 1.2, |
| 3151 | | shown at level 0.000798, step 1, values float32 |
| 3152 | | Opened W20_J150_run_body004.mrc as #31.4, grid size 240,240,240, pixel 1.2, |
| 3153 | | shown at level 0.000426, step 1, values float32 |
| 3154 | | |
| 3155 | | > select add #31 |
| 3156 | | |
| 3157 | | 9 models selected |
| 3158 | | |
| 3159 | | > ui mousemode right "rotate selected models" |
| 3160 | | |
| 3161 | | > view matrix models |
| 3162 | | > #31,0.62778,0.15918,-0.76194,138.56,0.19756,0.91423,0.35377,-64.97,0.7529,-0.37262,0.54248,29.724 |
| 3163 | | |
| 3164 | | > view matrix models |
| 3165 | | > #31,-0.3321,-0.94322,0.0062597,329.8,-0.75269,0.269,0.60092,127.95,-0.56848,0.19485,-0.79929,311.9 |
| 3166 | | |
| 3167 | | > ui mousemode right "translate selected models" |
| 3168 | | |
| 3169 | | > view matrix models |
| 3170 | | > #31,-0.3321,-0.94322,0.0062597,332.22,-0.75269,0.269,0.60092,127.27,-0.56848,0.19485,-0.79929,317.96 |
| 3171 | | |
| 3172 | | > fitmap #31.1 inMap #1 |
| 3173 | | |
| 3174 | | Fit map W20_J150_run_body001.mrc in map |
| 3175 | | W20_J3623_consensus_structure_sharp.mrc using 138218 points |
| 3176 | | correlation = 0.9172, correlation about mean = 0.7038, overlap = 893.5 |
| 3177 | | steps = 88, shift = 6.98, angle = 8.67 degrees |
| 3178 | | |
| 3179 | | Position of W20_J150_run_body001.mrc (#31.1) relative to |
| 3180 | | W20_J3623_consensus_structure_sharp.mrc (#1) coordinates: |
| 3181 | | Matrix rotation and translation |
| 3182 | | 0.99964242 -0.02165534 0.01568687 5.92000022 |
| 3183 | | 0.01908723 0.98871756 0.14857073 -17.88200850 |
| 3184 | | -0.01872723 -0.14821819 0.98877736 29.33526708 |
| 3185 | | Axis -0.98423540 0.11412684 0.13511379 |
| 3186 | | Axis point 0.00000000 191.49090363 131.90319589 |
| 3187 | | Rotation angle (degrees) 8.67162751 |
| 3188 | | Shift along axis -3.90389179 |
| 3189 | | |
| 3190 | | |
| 3191 | | > fitmap #31.2 inMap #1 |
| 3192 | | |
| 3193 | | Fit map W20_J150_run_body002.mrc in map |
| 3194 | | W20_J3623_consensus_structure_sharp.mrc using 138140 points |
| 3195 | | correlation = 0.8053, correlation about mean = 0.5992, overlap = 456.9 |
| 3196 | | steps = 116, shift = 12.3, angle = 8.44 degrees |
| 3197 | | |
| 3198 | | Position of W20_J150_run_body002.mrc (#31.2) relative to |
| 3199 | | W20_J3623_consensus_structure_sharp.mrc (#1) coordinates: |
| 3200 | | Matrix rotation and translation |
| 3201 | | 0.99987004 -0.01303295 0.00948916 5.64171510 |
| 3202 | | 0.01150966 0.98923255 0.14589889 -15.99717561 |
| 3203 | | -0.01128848 -0.14577072 0.98925400 27.73858221 |
| 3204 | | Axis -0.99397729 0.07080790 0.08363845 |
| 3205 | | Axis point 0.00000000 183.90016756 121.07225767 |
| 3206 | | Rotation angle (degrees) 8.43680332 |
| 3207 | | Shift along axis -4.42045109 |
| 3208 | | |
| 3209 | | |
| 3210 | | > fitmap #31.3 inMap #1 |
| 3211 | | |
| 3212 | | Fit map W20_J150_run_body003.mrc in map |
| 3213 | | W20_J3623_consensus_structure_sharp.mrc using 137514 points |
| 3214 | | correlation = 0.6146, correlation about mean = 0.266, overlap = 238.9 |
| 3215 | | steps = 124, shift = 9.37, angle = 6.56 degrees |
| 3216 | | |
| 3217 | | Position of W20_J150_run_body003.mrc (#31.3) relative to |
| 3218 | | W20_J3623_consensus_structure_sharp.mrc (#1) coordinates: |
| 3219 | | Matrix rotation and translation |
| 3220 | | 0.99927585 -0.03094085 0.02214582 5.52526341 |
| 3221 | | 0.02837142 0.99372585 0.10818506 -14.70532330 |
| 3222 | | -0.02535421 -0.10747841 0.99388408 23.92950955 |
| 3223 | | Axis -0.94316639 0.20773307 0.25939184 |
| 3224 | | Axis point 0.00000000 219.38003792 144.69692071 |
| 3225 | | Rotation angle (degrees) 6.56495264 |
| 3226 | | Shift along axis -2.05890526 |
| 3227 | | |
| 3228 | | |
| 3229 | | > fitmap #31.4 inMap #1 |
| 3230 | | |
| 3231 | | Fit map W20_J150_run_body004.mrc in map |
| 3232 | | W20_J3623_consensus_structure_sharp.mrc using 137710 points |
| 3233 | | correlation = 0.7262, correlation about mean = 0.4714, overlap = 200 |
| 3234 | | steps = 100, shift = 8.31, angle = 7.56 degrees |
| 3235 | | |
| 3236 | | Position of W20_J150_run_body004.mrc (#31.4) relative to |
| 3237 | | W20_J3623_consensus_structure_sharp.mrc (#1) coordinates: |
| 3238 | | Matrix rotation and translation |
| 3239 | | 0.99968731 -0.01039845 0.02274087 4.61807189 |
| 3240 | | 0.00737128 0.99156997 0.12936254 -14.95364310 |
| 3241 | | -0.02389433 -0.12915446 0.99133656 25.99366724 |
| 3242 | | Axis -0.98187156 0.17712480 0.06749108 |
| 3243 | | Axis point 0.00000000 195.98514242 120.93339845 |
| 3244 | | Rotation angle (degrees) 7.56466213 |
| 3245 | | Shift along axis -5.42867381 |
| 3246 | | |
| 3247 | | |
| 3248 | | > color #31 darkgrey models |
| 3249 | | |
| 3250 | | > color #31 #a9a9a980 models |
| 3251 | | |
| 3252 | | > select subtract #31 |
| 3253 | | |
| 3254 | | Nothing selected |
| 3255 | | |
| 3256 | | > hide #!31 models |
| 3257 | | |
| 3258 | | > rename #31 W20_J150_MB |
| 3259 | | |
| 3260 | | > open "/Users/becca/Desktop/Postdoc/COPI notebooks and |
| 3261 | | > resources/Processing/W20_MM_cyto_COPI/relionMB/W20_J155_run_ct6_body001.mrc" |
| 3262 | | > "/Users/becca/Desktop/Postdoc/COPI notebooks and |
| 3263 | | > resources/Processing/W20_MM_cyto_COPI/relionMB/W20_J155_run_ct6_body002.mrc" |
| 3264 | | > "/Users/becca/Desktop/Postdoc/COPI notebooks and |
| 3265 | | > resources/Processing/W20_MM_cyto_COPI/relionMB/W20_J155_run_ct6_body003.mrc" |
| 3266 | | > "/Users/becca/Desktop/Postdoc/COPI notebooks and |
| 3267 | | > resources/Processing/W20_MM_cyto_COPI/relionMB/W20_J155_run_ct6_body004.mrc" |
| 3268 | | |
| 3269 | | Opened W20_J155_run_ct6_body001.mrc as #32.1, grid size 240,240,240, pixel |
| 3270 | | 1.2, shown at level 0.0064, step 1, values float32 |
| 3271 | | Opened W20_J155_run_ct6_body002.mrc as #32.2, grid size 240,240,240, pixel |
| 3272 | | 1.2, shown at level 0.00156, step 1, values float32 |
| 3273 | | Opened W20_J155_run_ct6_body003.mrc as #32.3, grid size 240,240,240, pixel |
| 3274 | | 1.2, shown at level 0.000666, step 1, values float32 |
| 3275 | | Opened W20_J155_run_ct6_body004.mrc as #32.4, grid size 240,240,240, pixel |
| 3276 | | 1.2, shown at level 0.000439, step 1, values float32 |
| 3277 | | |
| 3278 | | > select add #32 |
| 3279 | | |
| 3280 | | 9 models selected |
| 3281 | | |
| 3282 | | > ui mousemode right "rotate selected models" |
| 3283 | | |
| 3284 | | > view matrix models |
| 3285 | | > #32,-0.094929,-0.99244,0.07775,390.6,-0.44492,0.11217,0.88852,96.076,-0.89053,0.049754,-0.45221,395 |
| 3286 | | |
| 3287 | | > ui mousemode right "translate selected models" |
| 3288 | | |
| 3289 | | > view matrix models |
| 3290 | | > #32,-0.094929,-0.99244,0.07775,372.3,-0.44492,0.11217,0.88852,92.438,-0.89053,0.049754,-0.45221,335.63 |
| 3291 | | |
| 3292 | | > ui mousemode right "translate selected models" |
| 3293 | | |
| 3294 | | > view matrix models |
| 3295 | | > #32,-0.094929,-0.99244,0.07775,368.31,-0.44492,0.11217,0.88852,91.761,-0.89053,0.049754,-0.45221,322.87 |
| 3296 | | |
| 3297 | | > ui mousemode right "rotate selected models" |
| 3298 | | |
| 3299 | | > view matrix models |
| 3300 | | > #32,0.24986,-0.92724,-0.27892,355.61,-0.7966,-0.36061,0.48518,310.1,-0.55046,0.10096,-0.82874,322.22 |
| 3301 | | |
| 3302 | | > ui mousemode right "translate selected models" |
| 3303 | | |
| 3304 | | > view matrix models |
| 3305 | | > #32,0.24986,-0.92724,-0.27892,326.75,-0.7966,-0.36061,0.48518,243.97,-0.55046,0.10096,-0.82874,318.12 |
| 3306 | | |
| 3307 | | > ui mousemode right "rotate selected models" |
| 3308 | | |
| 3309 | | > view matrix models |
| 3310 | | > #32,0.049187,-0.95013,-0.30796,371.28,-0.76011,-0.23563,0.60556,193.56,-0.64792,0.2043,-0.7338,299.66 |
| 3311 | | |
| 3312 | | > view matrix models |
| 3313 | | > #32,-0.38216,-0.71778,-0.58202,446.74,-0.57887,-0.30499,0.75623,147.95,-0.72032,0.62591,-0.29895,168.04 |
| 3314 | | |
| 3315 | | > view matrix models |
| 3316 | | > #32,-0.12174,-0.98206,-0.14398,378.68,-0.79719,0.01032,0.60364,153.76,-0.59133,0.18827,-0.78414,308.73 |
| 3317 | | |
| 3318 | | > ui mousemode right "translate selected models" |
| 3319 | | |
| 3320 | | > view matrix models |
| 3321 | | > #32,-0.12174,-0.98206,-0.14398,339.88,-0.79719,0.01032,0.60364,183.81,-0.59133,0.18827,-0.78414,311.67 |
| 3322 | | |
| 3323 | | > view matrix models |
| 3324 | | > #32,-0.12174,-0.98206,-0.14398,341.73,-0.79719,0.01032,0.60364,179.24,-0.59133,0.18827,-0.78414,317.95 |
| 3325 | | |
| 3326 | | > fitmap #32.4 inMap #1 |
| 3327 | | |
| 3328 | | Fit map W20_J155_run_ct6_body004.mrc in map |
| 3329 | | W20_J3623_consensus_structure_sharp.mrc using 137851 points |
| 3330 | | correlation = 0.7818, correlation about mean = 0.5257, overlap = 192.1 |
| 3331 | | steps = 124, shift = 4.21, angle = 14.8 degrees |
| 3332 | | |
| 3333 | | Position of W20_J155_run_ct6_body004.mrc (#32.4) relative to |
| 3334 | | W20_J3623_consensus_structure_sharp.mrc (#1) coordinates: |
| 3335 | | Matrix rotation and translation |
| 3336 | | 0.96755911 -0.24974155 0.03819070 46.04596253 |
| 3337 | | 0.24817341 0.96782709 0.04148106 -19.98854272 |
| 3338 | | -0.04732154 -0.03065746 0.99840913 9.25104536 |
| 3339 | | Axis -0.14135691 0.16756298 0.97567457 |
| 3340 | | Axis point 102.10235956 170.64051821 0.00000000 |
| 3341 | | Rotation angle (degrees) 14.78333075 |
| 3342 | | Shift along axis -0.83224502 |
| 3343 | | |
| 3344 | | |
| 3345 | | > fitmap #32.3 inMap #1 |
| 3346 | | |
| 3347 | | Fit map W20_J155_run_ct6_body003.mrc in map |
| 3348 | | W20_J3623_consensus_structure_sharp.mrc using 137790 points |
| 3349 | | correlation = 0.5288, correlation about mean = 0.2458, overlap = 222.1 |
| 3350 | | steps = 212, shift = 24.3, angle = 25.3 degrees |
| 3351 | | |
| 3352 | | Position of W20_J155_run_ct6_body003.mrc (#32.3) relative to |
| 3353 | | W20_J3623_consensus_structure_sharp.mrc (#1) coordinates: |
| 3354 | | Matrix rotation and translation |
| 3355 | | 0.96236345 -0.02996556 0.27010858 -46.55042113 |
| 3356 | | -0.06370212 0.94133951 0.33139398 -51.24613942 |
| 3357 | | -0.26419429 -0.33612794 0.90400187 97.79261178 |
| 3358 | | Axis -0.78009899 0.62441264 -0.03942621 |
| 3359 | | Axis point 0.00000000 234.71472833 197.05127274 |
| 3360 | | Rotation angle (degrees) 25.33083616 |
| 3361 | | Shift along axis 0.45960726 |
| 3362 | | |
| 3363 | | |
| 3364 | | > fitmap #32.2 inMap #1 |
| 3365 | | |
| 3366 | | Fit map W20_J155_run_ct6_body002.mrc in map |
| 3367 | | W20_J3623_consensus_structure_sharp.mrc using 138167 points |
| 3368 | | correlation = 0.8427, correlation about mean = 0.6574, overlap = 508.2 |
| 3369 | | steps = 200, shift = 28.2, angle = 13.9 degrees |
| 3370 | | |
| 3371 | | Position of W20_J155_run_ct6_body002.mrc (#32.2) relative to |
| 3372 | | W20_J3623_consensus_structure_sharp.mrc (#1) coordinates: |
| 3373 | | Matrix rotation and translation |
| 3374 | | 0.97079416 -0.23981830 0.00677387 48.62036376 |
| 3375 | | 0.23979745 0.97081570 0.00375114 -13.12837058 |
| 3376 | | -0.00747577 -0.00201723 0.99997002 1.33700507 |
| 3377 | | Axis -0.01202088 0.02969530 0.99948671 |
| 3378 | | Axis point 78.30820657 193.21986153 0.00000000 |
| 3379 | | Rotation angle (degrees) 13.88246944 |
| 3380 | | Shift along axis 0.36200848 |
| 3381 | | |
| 3382 | | |
| 3383 | | > fitmap #32.1 inMap #1 |
| 3384 | | |
| 3385 | | Fit map W20_J155_run_ct6_body001.mrc in map |
| 3386 | | W20_J3623_consensus_structure_sharp.mrc using 138158 points |
| 3387 | | correlation = 0.9152, correlation about mean = 0.7176, overlap = 887.9 |
| 3388 | | steps = 240, shift = 17.9, angle = 14.3 degrees |
| 3389 | | |
| 3390 | | Position of W20_J155_run_ct6_body001.mrc (#32.1) relative to |
| 3391 | | W20_J3623_consensus_structure_sharp.mrc (#1) coordinates: |
| 3392 | | Matrix rotation and translation |
| 3393 | | 0.96918769 -0.24592134 0.01406818 48.78106404 |
| 3394 | | 0.24563656 0.96916974 0.01930526 -16.53168875 |
| 3395 | | -0.01838203 -0.01525477 0.99971466 4.87447888 |
| 3396 | | Axis -0.06998243 0.06571014 0.99538165 |
| 3397 | | Axis point 90.87557164 187.07445802 0.00000000 |
| 3398 | | Rotation angle (degrees) 14.29528730 |
| 3399 | | Shift along axis 0.35184982 |
| 3400 | | |
| 3401 | | |
| 3402 | | > select add #32.3 |
| 3403 | | |
| 3404 | | 9 models selected |
| 3405 | | |
| 3406 | | > select subtract #32 |
| 3407 | | |
| 3408 | | Nothing selected |
| 3409 | | |
| 3410 | | > select subtract #32.3 |
| 3411 | | |
| 3412 | | Nothing selected |
| 3413 | | |
| 3414 | | > select add #32.3 |
| 3415 | | |
| 3416 | | 2 models selected |
| 3417 | | |
| 3418 | | > view matrix models |
| 3419 | | > #32.3,0.96236,-0.029966,0.27011,-49.146,-0.063702,0.94134,0.33139,-16.537,-0.26419,-0.33613,0.904,117.55 |
| 3420 | | |
| 3421 | | > fitmap #32.3 inMap #1 |
| 3422 | | |
| 3423 | | Fit map W20_J155_run_ct6_body003.mrc in map |
| 3424 | | W20_J3623_consensus_structure_sharp.mrc using 137790 points |
| 3425 | | correlation = 0.5773, correlation about mean = 0.2332, overlap = 255.7 |
| 3426 | | steps = 220, shift = 22.6, angle = 31.2 degrees |
| 3427 | | |
| 3428 | | Position of W20_J155_run_ct6_body003.mrc (#32.3) relative to |
| 3429 | | W20_J3623_consensus_structure_sharp.mrc (#1) coordinates: |
| 3430 | | Matrix rotation and translation |
| 3431 | | 0.93701301 -0.34494790 0.05493240 55.45886688 |
| 3432 | | 0.34457856 0.93861493 0.01635939 -26.98464957 |
| 3433 | | -0.05720351 0.00359956 0.99835605 3.33562233 |
| 3434 | | Axis -0.01826220 0.16049183 0.98686821 |
| 3435 | | Axis point 102.63951914 141.92416208 0.00000000 |
| 3436 | | Rotation angle (degrees) 20.44761418 |
| 3437 | | Shift along axis -2.05179723 |
| 3438 | | |
| 3439 | | |
| 3440 | | > select subtract #32.3 |
| 3441 | | |
| 3442 | | Nothing selected |
| 3443 | | |
| 3444 | | > color #32 #929292ff models |
| 3445 | | |
| 3446 | | > color #32 #9292927d models |
| 3447 | | |
| 3448 | | > hide #!32 models |
| 3449 | | |
| 3450 | | > rename #32 W20_J155_MB |
| 3451 | | |
| 3452 | | > open "/Users/becca/Desktop/Postdoc/COPI notebooks and |
| 3453 | | > resources/Processing/W20_MM_cyto_COPI/relionMB/W20_J160_run_ct6_body001.mrc" |
| 3454 | | > "/Users/becca/Desktop/Postdoc/COPI notebooks and |
| 3455 | | > resources/Processing/W20_MM_cyto_COPI/relionMB/W20_J160_run_ct6_body002.mrc" |
| 3456 | | > "/Users/becca/Desktop/Postdoc/COPI notebooks and |
| 3457 | | > resources/Processing/W20_MM_cyto_COPI/relionMB/W20_J160_run_ct6_body003.mrc" |
| 3458 | | > "/Users/becca/Desktop/Postdoc/COPI notebooks and |
| 3459 | | > resources/Processing/W20_MM_cyto_COPI/relionMB/W20_J160_run_ct6_body004.mrc" |
| 3460 | | |
| 3461 | | Opened W20_J160_run_ct6_body001.mrc as #33.1, grid size 240,240,240, pixel |
| 3462 | | 1.2, shown at level 0.00725, step 1, values float32 |
| 3463 | | Opened W20_J160_run_ct6_body002.mrc as #33.2, grid size 240,240,240, pixel |
| 3464 | | 1.2, shown at level 0.00154, step 1, values float32 |
| 3465 | | Opened W20_J160_run_ct6_body003.mrc as #33.3, grid size 240,240,240, pixel |
| 3466 | | 1.2, shown at level 0.000864, step 1, values float32 |
| 3467 | | Opened W20_J160_run_ct6_body004.mrc as #33.4, grid size 240,240,240, pixel |
| 3468 | | 1.2, shown at level 0.000379, step 1, values float32 |
| 3469 | | |
| 3470 | | > select add #33 |
| 3471 | | |
| 3472 | | 9 models selected |
| 3473 | | |
| 3474 | | > ui mousemode right "rotate selected models" |
| 3475 | | |
| 3476 | | > view matrix models |
| 3477 | | > #33,0.31873,-0.82608,0.46476,171.28,-0.72157,0.10647,0.6841,150.82,-0.6146,-0.5534,-0.56214,456.98 |
| 3478 | | |
| 3479 | | > view matrix models |
| 3480 | | > #33,-0.036679,-0.99898,-0.026385,337.23,-0.84584,0.016974,0.53317,211.08,-0.53218,0.041873,-0.8456,383.69 |
| 3481 | | |
| 3482 | | > ui mousemode right "translate selected models" |
| 3483 | | |
| 3484 | | > view matrix models |
| 3485 | | > #33,-0.036679,-0.99898,-0.026385,334.88,-0.84584,0.016974,0.53317,195.64,-0.53218,0.041873,-0.8456,333.69 |
| 3486 | | |
| 3487 | | > view matrix models |
| 3488 | | > #33,-0.036679,-0.99898,-0.026385,313.34,-0.84584,0.016974,0.53317,188.83,-0.53218,0.041873,-0.8456,328.42 |
| 3489 | | |
| 3490 | | > view matrix models |
| 3491 | | > #33,-0.036679,-0.99898,-0.026385,311.41,-0.84584,0.016974,0.53317,189.7,-0.53218,0.041873,-0.8456,344.34 |
| 3492 | | |
| 3493 | | > ui mousemode right "rotate selected models" |
| 3494 | | |
| 3495 | | > view matrix models |
| 3496 | | > #33,-0.35879,-0.93341,-0.0038469,340.33,-0.80584,0.30767,0.50593,135.86,-0.47106,0.18462,-0.86256,313.27 |
| 3497 | | |
| 3498 | | > ui mousemode right "translate selected models" |
| 3499 | | |
| 3500 | | > view matrix models |
| 3501 | | > #33,-0.35879,-0.93341,-0.0038469,338.34,-0.80584,0.30767,0.50593,141.61,-0.47106,0.18462,-0.86256,318.7 |
| 3502 | | |
| 3503 | | > fitmap #33.3 inMap #1 |
| 3504 | | |
| 3505 | | Fit map W20_J160_run_ct6_body003.mrc in map |
| 3506 | | W20_J3623_consensus_structure_sharp.mrc using 137937 points |
| 3507 | | correlation = 0.5867, correlation about mean = 0.2346, overlap = 250.3 |
| 3508 | | steps = 140, shift = 11.6, angle = 7.35 degrees |
| 3509 | | |
| 3510 | | Position of W20_J160_run_ct6_body003.mrc (#33.3) relative to |
| 3511 | | W20_J3623_consensus_structure_sharp.mrc (#1) coordinates: |
| 3512 | | Matrix rotation and translation |
| 3513 | | 0.99303756 0.05988139 0.10144267 -23.49545369 |
| 3514 | | -0.06488899 0.99679433 0.04680249 14.70011589 |
| 3515 | | -0.09831488 -0.05305914 0.99373986 28.54412294 |
| 3516 | | Axis -0.39036137 0.78085672 -0.48773024 |
| 3517 | | Axis point 309.26276930 0.00000000 226.94693512 |
| 3518 | | Rotation angle (degrees) 7.34879054 |
| 3519 | | Shift along axis 6.72856998 |
| 3520 | | |
| 3521 | | |
| 3522 | | > fitmap #33.2 inMap #1 |
| 3523 | | |
| 3524 | | Fit map W20_J160_run_ct6_body002.mrc in map |
| 3525 | | W20_J3623_consensus_structure_sharp.mrc using 137991 points |
| 3526 | | correlation = 0.819, correlation about mean = 0.6067, overlap = 480.4 |
| 3527 | | steps = 144, shift = 18.2, angle = 13.8 degrees |
| 3528 | | |
| 3529 | | Position of W20_J160_run_ct6_body002.mrc (#33.2) relative to |
| 3530 | | W20_J3623_consensus_structure_sharp.mrc (#1) coordinates: |
| 3531 | | Matrix rotation and translation |
| 3532 | | 0.99771963 -0.01489642 -0.06583042 17.27645423 |
| 3533 | | 0.02956838 0.97323369 0.22790766 -26.83710836 |
| 3534 | | 0.06067337 -0.22933444 0.97145481 35.68296509 |
| 3535 | | Axis -0.95958803 -0.26548631 0.09331575 |
| 3536 | | Axis point 0.00000000 140.48687556 141.38690851 |
| 3537 | | Rotation angle (degrees) 13.78322772 |
| 3538 | | Shift along axis -6.12361124 |
| 3539 | | |
| 3540 | | |
| 3541 | | > fitmap #33.1 inMap #1 |
| 3542 | | |
| 3543 | | Fit map W20_J160_run_ct6_body001.mrc in map |
| 3544 | | W20_J3623_consensus_structure_sharp.mrc using 138219 points |
| 3545 | | correlation = 0.9149, correlation about mean = 0.7061, overlap = 905.5 |
| 3546 | | steps = 240, shift = 9.92, angle = 13.4 degrees |
| 3547 | | |
| 3548 | | Position of W20_J160_run_ct6_body001.mrc (#33.1) relative to |
| 3549 | | W20_J3623_consensus_structure_sharp.mrc (#1) coordinates: |
| 3550 | | Matrix rotation and translation |
| 3551 | | 0.99744755 -0.02103927 -0.06823292 18.77482829 |
| 3552 | | 0.03561801 0.97482901 0.22009032 -27.49182319 |
| 3553 | | 0.06188489 -0.22195888 0.97309019 34.85093721 |
| 3554 | | Axis -0.95213476 -0.28026222 0.12203477 |
| 3555 | | Axis point 0.00000000 142.23926756 148.71521434 |
| 3556 | | Rotation angle (degrees) 13.42285005 |
| 3557 | | Shift along axis -5.91822131 |
| 3558 | | |
| 3559 | | |
| 3560 | | > fitmap #33.4 inMap #1 |
| 3561 | | |
| 3562 | | Fit map W20_J160_run_ct6_body004.mrc in map |
| 3563 | | W20_J3623_consensus_structure_sharp.mrc using 137797 points |
| 3564 | | correlation = 0.7345, correlation about mean = 0.436, overlap = 185.3 |
| 3565 | | steps = 144, shift = 10.1, angle = 13 degrees |
| 3566 | | |
| 3567 | | Position of W20_J160_run_ct6_body004.mrc (#33.4) relative to |
| 3568 | | W20_J3623_consensus_structure_sharp.mrc (#1) coordinates: |
| 3569 | | Matrix rotation and translation |
| 3570 | | 0.99800988 0.00249027 -0.06300862 14.90861204 |
| 3571 | | 0.01124609 0.97617108 0.21671073 -24.80457915 |
| 3572 | | 0.06204686 -0.21698805 0.97420038 33.68609528 |
| 3573 | | Axis -0.96067232 -0.27700640 0.01939474 |
| 3574 | | Axis point 0.00000000 139.38706533 138.47533972 |
| 3575 | | Rotation angle (degrees) 13.04561440 |
| 3576 | | Shift along axis -6.79793059 |
| 3577 | | |
| 3578 | | |
| 3579 | | > select subtract #33 |
| 3580 | | |
| 3581 | | Nothing selected |
| 3582 | | |
| 3583 | | > color #33 #929292ff models |
| 3584 | | |
| 3585 | | > color #33 #92929280 models |
| 3586 | | |
| 3587 | | > hide #!33 models |
| 3588 | | |
| 3589 | | > rename #33 W20_J160_MB |
| 3590 | | |
| 3591 | | > save "/Users/becca/Desktop/Postdoc/COPI notebooks and |
| 3592 | | > resources/Processing/W20_MM_cyto_COPI/20250701_W20_COPI_cyto_alignments.cxs" |
| 3593 | | |
| 3594 | | ——— End of log from Tue Jul 1 13:02:54 2025 ——— |
| 3595 | | |
| 3596 | | opened ChimeraX session |
| 3597 | | |
| 3598 | | > open "/Users/becca/Desktop/Postdoc/COPI notebooks and resources/Structure |
| 3599 | | > files/alphafold/CopA-F8WHL2.pdb" |
| 3600 | | |
| 3601 | | CopA-F8WHL2.pdb title: |
| 3602 | | Alphafold monomer V2.0 prediction for coatomer subunit α (F8WHL2) [more |
| 3603 | | info...] |
| 3604 | | |
| 3605 | | Chain information for CopA-F8WHL2.pdb #34 |
| 3606 | | --- |
| 3607 | | Chain | Description | UniProt |
| 3608 | | A | coatomer subunit α | F8WHL2_MOUSE 1-1233 |
| 3609 | | |
| 3610 | | Computing secondary structure |
| 3611 | | |
| 3612 | | > open "/Users/becca/Desktop/Postdoc/COPI notebooks and resources/Structure |
| 3613 | | > files/alphafold/CopB_ Q9JIF7.pdb" |
| 3614 | | |
| 3615 | | CopB_ Q9JIF7.pdb title: |
| 3616 | | Alphafold monomer V2.0 prediction for coatomer subunit β (Q9JIF7) [more |
| 3617 | | info...] |
| 3618 | | |
| 3619 | | Chain information for CopB_ Q9JIF7.pdb #35 |
| 3620 | | --- |
| 3621 | | Chain | Description | UniProt |
| 3622 | | A | coatomer subunit β | COPB_MOUSE 1-953 |
| 3623 | | |
| 3624 | | Computing secondary structure |
| 3625 | | |
| 3626 | | > open "/Users/becca/Desktop/Postdoc/COPI notebooks and resources/Structure |
| 3627 | | > files/alphafold/CopBprime_O55029.pdb" |
| 3628 | | |
| 3629 | | CopBprime_O55029.pdb title: |
| 3630 | | Alphafold monomer V2.0 prediction for coatomer subunit β' (O55029) [more |
| 3631 | | info...] |
| 3632 | | |
| 3633 | | Chain information for CopBprime_O55029.pdb #36 |
| 3634 | | --- |
| 3635 | | Chain | Description | UniProt |
| 3636 | | A | coatomer subunit β' | COPB2_MOUSE 1-905 |
| 3637 | | |
| 3638 | | Computing secondary structure |
| 3639 | | |
| 3640 | | > open "/Users/becca/Desktop/Postdoc/COPI notebooks and resources/Structure |
| 3641 | | > files/alphafold/CopD_full_Q5XJY5.pdb" |
| 3642 | | |
| 3643 | | CopD_full_Q5XJY5.pdb title: |
| 3644 | | Alphafold monomer V2.0 prediction for coatomer subunit δ (Q5XJY5) [more |
| 3645 | | info...] |
| 3646 | | |
| 3647 | | Chain information for CopD_full_Q5XJY5.pdb #37 |
| 3648 | | --- |
| 3649 | | Chain | Description | UniProt |
| 3650 | | A | coatomer subunit δ | COPD_MOUSE 1-511 |
| 3651 | | |
| 3652 | | Computing secondary structure |
| 3653 | | |
| 3654 | | > open "/Users/becca/Desktop/Postdoc/COPI notebooks and resources/Structure |
| 3655 | | > files/alphafold/CopG1_ Q9QZE5.pdb" |
| 3656 | | |
| 3657 | | CopG1_ Q9QZE5.pdb title: |
| 3658 | | Alphafold monomer V2.0 prediction for coatomer subunit γ-1 (Q9QZE5) [more |
| 3659 | | info...] |
| 3660 | | |
| 3661 | | Chain information for CopG1_ Q9QZE5.pdb #38 |
| 3662 | | --- |
| 3663 | | Chain | Description | UniProt |
| 3664 | | A | coatomer subunit γ-1 | COPG1_MOUSE 1-874 |
| 3665 | | |
| 3666 | | Computing secondary structure |
| 3667 | | |
| 3668 | | > open "/Users/becca/Desktop/Postdoc/COPI notebooks and resources/Structure |
| 3669 | | > files/alphafold/CopG1_ Q9QZE5.pdb" |
| 3670 | | |
| 3671 | | CopG1_ Q9QZE5.pdb title: |
| 3672 | | Alphafold monomer V2.0 prediction for coatomer subunit γ-1 (Q9QZE5) [more |
| 3673 | | info...] |
| 3674 | | |
| 3675 | | Chain information for CopG1_ Q9QZE5.pdb #39 |
| 3676 | | --- |
| 3677 | | Chain | Description | UniProt |
| 3678 | | A | coatomer subunit γ-1 | COPG1_MOUSE 1-874 |
| 3679 | | |
| 3680 | | Computing secondary structure |
| 3681 | | |
| 3682 | | > open "/Users/becca/Desktop/Postdoc/COPI notebooks and resources/Structure |
| 3683 | | > files/alphafold/CopZ1_P61924.pdb" |
| 3684 | | |
| 3685 | | CopZ1_P61924.pdb title: |
| 3686 | | Alphafold monomer V2.0 prediction for coatomer subunit ζ-1 (P61924) [more |
| 3687 | | info...] |
| 3688 | | |
| 3689 | | Chain information for CopZ1_P61924.pdb #40 |
| 3690 | | --- |
| 3691 | | Chain | Description | UniProt |
| 3692 | | A | coatomer subunit ζ-1 | COPZ1_MOUSE 1-177 |
| 3693 | | |
| 3694 | | Computing secondary structure |
| 3695 | | |
| 3696 | | > hide #40 models |
| 3697 | | |
| 3698 | | > hide #39 models |
| 3699 | | |
| 3700 | | > hide #38 models |
| 3701 | | |
| 3702 | | > hide #37 models |
| 3703 | | |
| 3704 | | > hide #36 models |
| 3705 | | |
| 3706 | | > hide #35 models |
| 3707 | | |
| 3708 | | > select add #34 |
| 3709 | | |
| 3710 | | 9810 atoms, 10032 bonds, 1233 residues, 1 model selected |
| 3711 | | |
| 3712 | | > color list |
| 3713 | | |
| 3714 | | 28 custom colors: alphav1 |
| 3715 | | |
| 3716 | | , alphav2 |
| 3717 | | |
| 3718 | | , alphav3 |
| 3719 | | |
| 3720 | | , arf1v1 |
| 3721 | | |
| 3722 | | , arf1v2 |
| 3723 | | |
| 3724 | | , arf1v3 |
| 3725 | | |
| 3726 | | , betapv1 |
| 3727 | | |
| 3728 | | , betapv2 |
| 3729 | | |
| 3730 | | , betapv3 |
| 3731 | | |
| 3732 | | , betav1 |
| 3733 | | |
| 3734 | | , betav2 |
| 3735 | | |
| 3736 | | , betav3 |
| 3737 | | |
| 3738 | | , deltav1 |
| 3739 | | |
| 3740 | | , deltav2 |
| 3741 | | |
| 3742 | | , deltav3 |
| 3743 | | |
| 3744 | | , epsilonv1 |
| 3745 | | |
| 3746 | | , epsilonv3 |
| 3747 | | |
| 3748 | | , gammav1 |
| 3749 | | |
| 3750 | | , gammav2 |
| 3751 | | |
| 3752 | | , gammav3 |
| 3753 | | |
| 3754 | | , label_purple |
| 3755 | | |
| 3756 | | , label_purple_v3 |
| 3757 | | |
| 3758 | | , label_red |
| 3759 | | |
| 3760 | | , label_red_v3 |
| 3761 | | |
| 3762 | | , lable_purple_v4 |
| 3763 | | |
| 3764 | | , zetav1 |
| 3765 | | |
| 3766 | | , zetav2 |
| 3767 | | |
| 3768 | | , and zetav3 |
| 3769 | | |
| 3770 | | . |
| 3771 | | 248 builtin colors: alice blue |
| 3772 | | |
| 3773 | | , aliceblue |
| 3774 | | |
| 3775 | | , antique white |
| 3776 | | |
| 3777 | | , antiquewhite |
| 3778 | | |
| 3779 | | , aqua |
| 3780 | | |
| 3781 | | , aquamarine |
| 3782 | | |
| 3783 | | , azure |
| 3784 | | |
| 3785 | | , beige |
| 3786 | | |
| 3787 | | , bisque |
| 3788 | | |
| 3789 | | , black |
| 3790 | | |
| 3791 | | , blanched almond |
| 3792 | | |
| 3793 | | , blanchedalmond |
| 3794 | | |
| 3795 | | , blue |
| 3796 | | |
| 3797 | | , blue violet |
| 3798 | | |
| 3799 | | , blueviolet |
| 3800 | | |
| 3801 | | , brown |
| 3802 | | |
| 3803 | | , burly wood |
| 3804 | | |
| 3805 | | , burlywood |
| 3806 | | |
| 3807 | | , cadet blue |
| 3808 | | |
| 3809 | | , cadetblue |
| 3810 | | |
| 3811 | | , chartreuse |
| 3812 | | |
| 3813 | | , chocolate |
| 3814 | | |
| 3815 | | , coral |
| 3816 | | |
| 3817 | | , cornflower blue |
| 3818 | | |
| 3819 | | , cornflowerblue |
| 3820 | | |
| 3821 | | , cornsilk |
| 3822 | | |
| 3823 | | , crimson |
| 3824 | | |
| 3825 | | , cyan |
| 3826 | | |
| 3827 | | , dark blue |
| 3828 | | |
| 3829 | | , dark cyan |
| 3830 | | |
| 3831 | | , dark goldenrod |
| 3832 | | |
| 3833 | | , dark gray |
| 3834 | | |
| 3835 | | , dark green |
| 3836 | | |
| 3837 | | , dark grey |
| 3838 | | |
| 3839 | | , dark khaki |
| 3840 | | |
| 3841 | | , dark magenta |
| 3842 | | |
| 3843 | | , dark olive green |
| 3844 | | |
| 3845 | | , dark orange |
| 3846 | | |
| 3847 | | , dark orchid |
| 3848 | | |
| 3849 | | , dark red |
| 3850 | | |
| 3851 | | , dark salmon |
| 3852 | | |
| 3853 | | , dark sea green |
| 3854 | | |
| 3855 | | , dark seagreen |
| 3856 | | |
| 3857 | | , dark slate blue |
| 3858 | | |
| 3859 | | , dark slate gray |
| 3860 | | |
| 3861 | | , dark slate grey |
| 3862 | | |
| 3863 | | , dark turquoise |
| 3864 | | |
| 3865 | | , dark violet |
| 3866 | | |
| 3867 | | , darkblue |
| 3868 | | |
| 3869 | | , darkcyan |
| 3870 | | |
| 3871 | | , darkgoldenrod |
| 3872 | | |
| 3873 | | , darkgray |
| 3874 | | |
| 3875 | | , darkgreen |
| 3876 | | |
| 3877 | | , darkgrey |
| 3878 | | |
| 3879 | | , darkkhaki |
| 3880 | | |
| 3881 | | , darkmagenta |
| 3882 | | |
| 3883 | | , darkolivegreen |
| 3884 | | |
| 3885 | | , darkorange |
| 3886 | | |
| 3887 | | , darkorchid |
| 3888 | | |
| 3889 | | , darkred |
| 3890 | | |
| 3891 | | , darksalmon |
| 3892 | | |
| 3893 | | , darkseagreen |
| 3894 | | |
| 3895 | | , darkslateblue |
| 3896 | | |
| 3897 | | , darkslategray |
| 3898 | | |
| 3899 | | , darkslategrey |
| 3900 | | |
| 3901 | | , darkturquoise |
| 3902 | | |
| 3903 | | , darkviolet |
| 3904 | | |
| 3905 | | , deep pink |
| 3906 | | |
| 3907 | | , deep sky blue |
| 3908 | | |
| 3909 | | , deep skyblue |
| 3910 | | |
| 3911 | | , deeppink |
| 3912 | | |
| 3913 | | , deepskyblue |
| 3914 | | |
| 3915 | | , dim gray |
| 3916 | | |
| 3917 | | , dim grey |
| 3918 | | |
| 3919 | | , dimgray |
| 3920 | | |
| 3921 | | , dimgrey |
| 3922 | | |
| 3923 | | , dodger blue |
| 3924 | | |
| 3925 | | , dodgerblue |
| 3926 | | |
| 3927 | | , fire brick |
| 3928 | | |
| 3929 | | , firebrick |
| 3930 | | |
| 3931 | | , floral white |
| 3932 | | |
| 3933 | | , floralwhite |
| 3934 | | |
| 3935 | | , forest green |
| 3936 | | |
| 3937 | | , forestgreen |
| 3938 | | |
| 3939 | | , fuchsia |
| 3940 | | |
| 3941 | | , gainsboro |
| 3942 | | |
| 3943 | | , ghost white |
| 3944 | | |
| 3945 | | , ghostwhite |
| 3946 | | |
| 3947 | | , gold |
| 3948 | | |
| 3949 | | , goldenrod |
| 3950 | | |
| 3951 | | , gray |
| 3952 | | |
| 3953 | | , green |
| 3954 | | |
| 3955 | | , green yellow |
| 3956 | | |
| 3957 | | , greenyellow |
| 3958 | | |
| 3959 | | , grey |
| 3960 | | |
| 3961 | | , honeydew |
| 3962 | | |
| 3963 | | , hot pink |
| 3964 | | |
| 3965 | | , hotpink |
| 3966 | | |
| 3967 | | , indian red |
| 3968 | | |
| 3969 | | , indianred |
| 3970 | | |
| 3971 | | , indigo |
| 3972 | | |
| 3973 | | , ivory |
| 3974 | | |
| 3975 | | , khaki |
| 3976 | | |
| 3977 | | , lavender |
| 3978 | | |
| 3979 | | , lavender blush |
| 3980 | | |
| 3981 | | , lavenderblush |
| 3982 | | |
| 3983 | | , lawn green |
| 3984 | | |
| 3985 | | , lawngreen |
| 3986 | | |
| 3987 | | , lemon chiffon |
| 3988 | | |
| 3989 | | , lemonchiffon |
| 3990 | | |
| 3991 | | , light blue |
| 3992 | | |
| 3993 | | , light coral |
| 3994 | | |
| 3995 | | , light cyan |
| 3996 | | |
| 3997 | | , light goldenrod yellow |
| 3998 | | |
| 3999 | | , light gray |
| 4000 | | |
| 4001 | | , light green |
| 4002 | | |
| 4003 | | , light grey |
| 4004 | | |
| 4005 | | , light pink |
| 4006 | | |
| 4007 | | , light salmon |
| 4008 | | |
| 4009 | | , light sea green |
| 4010 | | |
| 4011 | | , light seagreen |
| 4012 | | |
| 4013 | | , light sky blue |
| 4014 | | |
| 4015 | | , light skyblue |
| 4016 | | |
| 4017 | | , light slate gray |
| 4018 | | |
| 4019 | | , light slate grey |
| 4020 | | |
| 4021 | | , light steel blue |
| 4022 | | |
| 4023 | | , light yellow |
| 4024 | | |
| 4025 | | , lightblue |
| 4026 | | |
| 4027 | | , lightcoral |
| 4028 | | |
| 4029 | | , lightcyan |
| 4030 | | |
| 4031 | | , lightgoldenrodyellow |
| 4032 | | |
| 4033 | | , lightgray |
| 4034 | | |
| 4035 | | , lightgreen |
| 4036 | | |
| 4037 | | , lightgrey |
| 4038 | | |
| 4039 | | , lightpink |
| 4040 | | |
| 4041 | | , lightsalmon |
| 4042 | | |
| 4043 | | , lightseagreen |
| 4044 | | |
| 4045 | | , lightskyblue |
| 4046 | | |
| 4047 | | , lightslategray |
| 4048 | | |
| 4049 | | , lightslategrey |
| 4050 | | |
| 4051 | | , lightsteelblue |
| 4052 | | |
| 4053 | | , lightyellow |
| 4054 | | |
| 4055 | | , lime |
| 4056 | | |
| 4057 | | , lime green |
| 4058 | | |
| 4059 | | , limegreen |
| 4060 | | |
| 4061 | | , linen |
| 4062 | | |
| 4063 | | , magenta |
| 4064 | | |
| 4065 | | , maroon |
| 4066 | | |
| 4067 | | , medium aquamarine |
| 4068 | | |
| 4069 | | , medium blue |
| 4070 | | |
| 4071 | | , medium orchid |
| 4072 | | |
| 4073 | | , medium purple |
| 4074 | | |
| 4075 | | , medium sea green |
| 4076 | | |
| 4077 | | , medium seagreen |
| 4078 | | |
| 4079 | | , medium slate blue |
| 4080 | | |
| 4081 | | , medium spring green |
| 4082 | | |
| 4083 | | , medium turquoise |
| 4084 | | |
| 4085 | | , medium violet red |
| 4086 | | |
| 4087 | | , mediumaquamarine |
| 4088 | | |
| 4089 | | , mediumblue |
| 4090 | | |
| 4091 | | , mediumorchid |
| 4092 | | |
| 4093 | | , mediumpurple |
| 4094 | | |
| 4095 | | , mediumseagreen |
| 4096 | | |
| 4097 | | , mediumslateblue |
| 4098 | | |
| 4099 | | , mediumspringgreen |
| 4100 | | |
| 4101 | | , mediumturquoise |
| 4102 | | |
| 4103 | | , mediumvioletred |
| 4104 | | |
| 4105 | | , midnight blue |
| 4106 | | |
| 4107 | | , midnightblue |
| 4108 | | |
| 4109 | | , mint cream |
| 4110 | | |
| 4111 | | , mintcream |
| 4112 | | |
| 4113 | | , misty rose |
| 4114 | | |
| 4115 | | , mistyrose |
| 4116 | | |
| 4117 | | , moccasin |
| 4118 | | |
| 4119 | | , navajo white |
| 4120 | | |
| 4121 | | , navajowhite |
| 4122 | | |
| 4123 | | , navy |
| 4124 | | |
| 4125 | | , old lace |
| 4126 | | |
| 4127 | | , oldlace |
| 4128 | | |
| 4129 | | , olive |
| 4130 | | |
| 4131 | | , olive drab |
| 4132 | | |
| 4133 | | , olivedrab |
| 4134 | | |
| 4135 | | , orange |
| 4136 | | |
| 4137 | | , orange red |
| 4138 | | |
| 4139 | | , orangered |
| 4140 | | |
| 4141 | | , orchid |
| 4142 | | |
| 4143 | | , pale goldenrod |
| 4144 | | |
| 4145 | | , pale green |
| 4146 | | |
| 4147 | | , pale turquoise |
| 4148 | | |
| 4149 | | , pale violet red |
| 4150 | | |
| 4151 | | , palegoldenrod |
| 4152 | | |
| 4153 | | , palegreen |
| 4154 | | |
| 4155 | | , paleturquoise |
| 4156 | | |
| 4157 | | , palevioletred |
| 4158 | | |
| 4159 | | , papaya whip |
| 4160 | | |
| 4161 | | , papayawhip |
| 4162 | | |
| 4163 | | , peach puff |
| 4164 | | |
| 4165 | | , peachpuff |
| 4166 | | |
| 4167 | | , peru |
| 4168 | | |
| 4169 | | , pink |
| 4170 | | |
| 4171 | | , plum |
| 4172 | | |
| 4173 | | , powder blue |
| 4174 | | |
| 4175 | | , powderblue |
| 4176 | | |
| 4177 | | , purple |
| 4178 | | |
| 4179 | | , rebecca purple |
| 4180 | | |
| 4181 | | , rebeccapurple |
| 4182 | | |
| 4183 | | , red |
| 4184 | | |
| 4185 | | , rosy brown |
| 4186 | | |
| 4187 | | , rosybrown |
| 4188 | | |
| 4189 | | , royal blue |
| 4190 | | |
| 4191 | | , royalblue |
| 4192 | | |
| 4193 | | , saddle brown |
| 4194 | | |
| 4195 | | , saddlebrown |
| 4196 | | |
| 4197 | | , salmon |
| 4198 | | |
| 4199 | | , sandy brown |
| 4200 | | |
| 4201 | | , sandybrown |
| 4202 | | |
| 4203 | | , sea green |
| 4204 | | |
| 4205 | | , seagreen |
| 4206 | | |
| 4207 | | , seashell |
| 4208 | | |
| 4209 | | , sienna |
| 4210 | | |
| 4211 | | , silver |
| 4212 | | |
| 4213 | | , sky blue |
| 4214 | | |
| 4215 | | , skyblue |
| 4216 | | |
| 4217 | | , slate blue |
| 4218 | | |
| 4219 | | , slate gray |
| 4220 | | |
| 4221 | | , slate grey |
| 4222 | | |
| 4223 | | , slateblue |
| 4224 | | |
| 4225 | | , slategray |
| 4226 | | |
| 4227 | | , slategrey |
| 4228 | | |
| 4229 | | , snow |
| 4230 | | |
| 4231 | | , spring green |
| 4232 | | |
| 4233 | | , springgreen |
| 4234 | | |
| 4235 | | , steel blue |
| 4236 | | |
| 4237 | | , steelblue |
| 4238 | | |
| 4239 | | , tan |
| 4240 | | |
| 4241 | | , teal |
| 4242 | | |
| 4243 | | , thistle |
| 4244 | | |
| 4245 | | , tomato |
| 4246 | | |
| 4247 | | , transparent |
| 4248 | | |
| 4249 | | , turquoise |
| 4250 | | |
| 4251 | | , violet |
| 4252 | | |
| 4253 | | , wheat |
| 4254 | | |
| 4255 | | , white |
| 4256 | | |
| 4257 | | , white smoke |
| 4258 | | |
| 4259 | | , whitesmoke |
| 4260 | | |
| 4261 | | , yellow |
| 4262 | | |
| 4263 | | , yellow green |
| 4264 | | |
| 4265 | | , and yellowgreen |
| 4266 | | |
| 4267 | | . |
| 4268 | | |
| 4269 | | > color sel navy |
| 4270 | | |
| 4271 | | > select subtract #34 |
| 4272 | | |
| 4273 | | Nothing selected |
| 4274 | | |
| 4275 | | > hide #34 models |
| 4276 | | |
| 4277 | | > show #35 models |
| 4278 | | |
| 4279 | | > select add #35 |
| 4280 | | |
| 4281 | | 7501 atoms, 7619 bonds, 953 residues, 1 model selected |
| 4282 | | |
| 4283 | | > color sel betav1 |
| 4284 | | |
| 4285 | | > select subtract #35 |
| 4286 | | |
| 4287 | | Nothing selected |
| 4288 | | |
| 4289 | | > hide #35 models |
| 4290 | | |
| 4291 | | > show #36 models |
| 4292 | | |
| 4293 | | > select add #36 |
| 4294 | | |
| 4295 | | 7214 atoms, 7373 bonds, 905 residues, 1 model selected |
| 4296 | | |
| 4297 | | > color sel betapv2 |
| 4298 | | |
| 4299 | | > color sel betapv3 |
| 4300 | | |
| 4301 | | > select subtract #36 |
| 4302 | | |
| 4303 | | Nothing selected |
| 4304 | | |
| 4305 | | > hide #36 models |
| 4306 | | |
| 4307 | | > show #37 models |
| 4308 | | |
| 4309 | | > select add #37 |
| 4310 | | |
| 4311 | | 4014 atoms, 4083 bonds, 511 residues, 1 model selected |
| 4312 | | |
| 4313 | | > color sel deltav3 |
| 4314 | | |
| 4315 | | > select subtract #37 |
| 4316 | | |
| 4317 | | Nothing selected |
| 4318 | | |
| 4319 | | > hide #37 models |
| 4320 | | |
| 4321 | | > show #38 models |
| 4322 | | |
| 4323 | | > select add #38 |
| 4324 | | |
| 4325 | | 6830 atoms, 6947 bonds, 874 residues, 1 model selected |
| 4326 | | |
| 4327 | | > close #39 |
| 4328 | | |
| 4329 | | > close #40 |
| 4330 | | |
| 4331 | | > open "/Users/becca/Desktop/Postdoc/COPI notebooks and resources/Structure |
| 4332 | | > files/alphafold/CopZ1_P61924.pdb" |
| 4333 | | |
| 4334 | | CopZ1_P61924.pdb title: |
| 4335 | | Alphafold monomer V2.0 prediction for coatomer subunit ζ-1 (P61924) [more |
| 4336 | | info...] |
| 4337 | | |
| 4338 | | Chain information for CopZ1_P61924.pdb #39 |
| 4339 | | --- |
| 4340 | | Chain | Description | UniProt |
| 4341 | | A | coatomer subunit ζ-1 | COPZ1_MOUSE 1-177 |
| 4342 | | |
| 4343 | | Computing secondary structure |
| 4344 | | |
| 4345 | | > hide #39 models |
| 4346 | | |
| 4347 | | > color sel gammav3 |
| 4348 | | |
| 4349 | | > select subtract #38 |
| 4350 | | |
| 4351 | | Nothing selected |
| 4352 | | |
| 4353 | | > hide #38 models |
| 4354 | | |
| 4355 | | > show #39 models |
| 4356 | | |
| 4357 | | > select add #39 |
| 4358 | | |
| 4359 | | 1420 atoms, 1441 bonds, 177 residues, 1 model selected |
| 4360 | | |
| 4361 | | > zetav3 |
| 4362 | | |
| 4363 | | Unknown command: zetav3 |
| 4364 | | |
| 4365 | | > color sel zetav3 |
| 4366 | | |
| 4367 | | > select subtract #39 |
| 4368 | | |
| 4369 | | Nothing selected |
| 4370 | | |
| 4371 | | > hide #39 models |
| 4372 | | |
| 4373 | | > show #34 models |
| 4374 | | |
| 4375 | | > select add #34 |
| 4376 | | |
| 4377 | | 9810 atoms, 10032 bonds, 1233 residues, 1 model selected |
| 4378 | | |
| 4379 | | > view matrix models #34,1,0,0,39.828,0,1,0,139.02,0,0,1,198.43 |
| 4380 | | |
| 4381 | | > ui mousemode right "rotate selected models" |
| 4382 | | |
| 4383 | | > view matrix models |
| 4384 | | > #34,0.99004,0.0096002,0.14042,38.534,-0.012281,0.99976,0.018237,138.66,-0.14022,-0.01978,0.98992,196.08 |
| 4385 | | |
| 4386 | | > view matrix models |
| 4387 | | > #34,0.13783,-0.38457,0.91275,20.074,-0.096945,0.91188,0.39884,134.94,-0.9857,-0.14346,0.088401,188.13 |
| 4388 | | |
| 4389 | | > view matrix models |
| 4390 | | > #34,-0.34037,-0.83282,0.43653,18.135,0.30375,0.34197,0.88926,142.89,-0.88988,0.43527,0.13658,185.25 |
| 4391 | | |
| 4392 | | > ui mousemode right "translate selected models" |
| 4393 | | |
| 4394 | | > view matrix models |
| 4395 | | > #34,-0.34037,-0.83282,0.43653,139.16,0.30375,0.34197,0.88926,104.42,-0.88988,0.43527,0.13658,153.4 |
| 4396 | | |
| 4397 | | > view matrix models |
| 4398 | | > #34,-0.34037,-0.83282,0.43653,161.88,0.30375,0.34197,0.88926,148.44,-0.88988,0.43527,0.13658,135.72 |
| 4399 | | |
| 4400 | | > ui mousemode right "rotate selected models" |
| 4401 | | |
| 4402 | | > view matrix models |
| 4403 | | > #34,-0.64868,-0.43267,-0.62611,161.12,0.47729,-0.87206,0.10814,166.41,-0.59279,-0.22869,0.7722,141.4 |
| 4404 | | |
| 4405 | | > ui mousemode right "translate selected models" |
| 4406 | | |
| 4407 | | > view matrix models |
| 4408 | | > #34,-0.64868,-0.43267,-0.62611,149.78,0.47729,-0.87206,0.10814,138.8,-0.59279,-0.22869,0.7722,155.26 |
| 4409 | | |
| 4410 | | > fitmap #34 inMap #1 |
| 4411 | | |
| 4412 | | Fit molecule CopA-F8WHL2.pdb (#34) to map |
| 4413 | | W20_J3623_consensus_structure_sharp.mrc (#1) using 9810 atoms |
| 4414 | | average map value = 0.1956, steps = 172 |
| 4415 | | shifted from previous position = 12.4 |
| 4416 | | rotated from previous position = 16.7 degrees |
| 4417 | | atoms outside contour = 6664, contour level = 0.26383 |
| 4418 | | |
| 4419 | | Position of CopA-F8WHL2.pdb (#34) relative to |
| 4420 | | W20_J3623_consensus_structure_sharp.mrc (#1) coordinates: |
| 4421 | | Matrix rotation and translation |
| 4422 | | -0.40275740 -0.50265546 -0.76493398 155.53344658 |
| 4423 | | 0.55537393 -0.79849851 0.23229283 137.87047167 |
| 4424 | | -0.72756189 -0.33126673 0.60076291 143.99545516 |
| 4425 | | Axis -0.46989046 -0.03116049 0.88217458 |
| 4426 | | Axis point 89.79461102 105.24732460 0.00000000 |
| 4427 | | Rotation angle (degrees) 143.15364698 |
| 4428 | | Shift along axis 49.64933605 |
| 4429 | | |
| 4430 | | |
| 4431 | | > view matrix models |
| 4432 | | > #34,-0.40276,-0.50266,-0.76493,152.08,0.55537,-0.7985,0.23229,129.79,-0.72756,-0.33127,0.60076,131.15 |
| 4433 | | |
| 4434 | | > fitmap #34 inMap #1 |
| 4435 | | |
| 4436 | | Fit molecule CopA-F8WHL2.pdb (#34) to map |
| 4437 | | W20_J3623_consensus_structure_sharp.mrc (#1) using 9810 atoms |
| 4438 | | average map value = 0.3026, steps = 204 |
| 4439 | | shifted from previous position = 8.55 |
| 4440 | | rotated from previous position = 12.6 degrees |
| 4441 | | atoms outside contour = 4835, contour level = 0.26383 |
| 4442 | | |
| 4443 | | Position of CopA-F8WHL2.pdb (#34) relative to |
| 4444 | | W20_J3623_consensus_structure_sharp.mrc (#1) coordinates: |
| 4445 | | Matrix rotation and translation |
| 4446 | | -0.54728967 -0.56189790 -0.62027800 158.57511981 |
| 4447 | | 0.58976792 -0.78477262 0.19054069 127.65605082 |
| 4448 | | -0.59384159 -0.26153911 0.76088730 134.18075471 |
| 4449 | | Axis -0.36531666 -0.02136273 0.93063815 |
| 4450 | | Axis point 81.60160811 99.25852793 0.00000000 |
| 4451 | | Rotation angle (degrees) 141.77504660 |
| 4452 | | Shift along axis 64.21651520 |
| 4453 | | |
| 4454 | | |
| 4455 | | > select subtract #34 |
| 4456 | | |
| 4457 | | Nothing selected |
| 4458 | | |
| 4459 | | > hide #34 models |
| 4460 | | |
| 4461 | | > show #35 models |
| 4462 | | |
| 4463 | | > select add #35 |
| 4464 | | |
| 4465 | | 7501 atoms, 7619 bonds, 953 residues, 1 model selected |
| 4466 | | |
| 4467 | | > view matrix models #35,1,0,0,116.88,0,1,0,-7.2403,0,0,1,111.96 |
| 4468 | | |
| 4469 | | > view matrix models #35,1,0,0,112.47,0,1,0,89.143,0,0,1,196.06 |
| 4470 | | |
| 4471 | | > ui mousemode right "rotate selected models" |
| 4472 | | |
| 4473 | | > view matrix models |
| 4474 | | > #35,0.0065824,0.90646,-0.42225,117.47,0.83324,-0.23843,-0.49886,91.055,-0.55287,-0.34855,-0.75686,209.75 |
| 4475 | | |
| 4476 | | > ui mousemode right "translate selected models" |
| 4477 | | |
| 4478 | | > view matrix models |
| 4479 | | > #35,0.0065824,0.90646,-0.42225,96.987,0.83324,-0.23843,-0.49886,131.53,-0.55287,-0.34855,-0.75686,188.84 |
| 4480 | | |
| 4481 | | > view matrix models |
| 4482 | | > #35,0.0065824,0.90646,-0.42225,132.29,0.83324,-0.23843,-0.49886,123.6,-0.55287,-0.34855,-0.75686,174.35 |
| 4483 | | |
| 4484 | | > view matrix models |
| 4485 | | > #35,0.0065824,0.90646,-0.42225,129.73,0.83324,-0.23843,-0.49886,153.17,-0.55287,-0.34855,-0.75686,162.06 |
| 4486 | | |
| 4487 | | > fitmap #35 inMap #1 |
| 4488 | | |
| 4489 | | Fit molecule CopB_ Q9JIF7.pdb (#35) to map |
| 4490 | | W20_J3623_consensus_structure_sharp.mrc (#1) using 7501 atoms |
| 4491 | | average map value = 0.272, steps = 140 |
| 4492 | | shifted from previous position = 13 |
| 4493 | | rotated from previous position = 19.4 degrees |
| 4494 | | atoms outside contour = 4336, contour level = 0.26383 |
| 4495 | | |
| 4496 | | Position of CopB_ Q9JIF7.pdb (#35) relative to |
| 4497 | | W20_J3623_consensus_structure_sharp.mrc (#1) coordinates: |
| 4498 | | Matrix rotation and translation |
| 4499 | | 0.29878946 0.85180997 -0.43028434 135.66407916 |
| 4500 | | 0.69839777 -0.50244520 -0.50969537 144.97471611 |
| 4501 | | -0.65035790 -0.14821802 -0.74502752 155.00132184 |
| 4502 | | Axis 0.80301824 0.48889117 -0.34080365 |
| 4503 | | Axis point 0.00000000 17.11569470 112.17244297 |
| 4504 | | Rotation angle (degrees) 166.99275965 |
| 4505 | | Shift along axis 126.99257230 |
| 4506 | | |
| 4507 | | |
| 4508 | | > select subtract #35 |
| 4509 | | |
| 4510 | | Nothing selected |
| 4511 | | |
| 4512 | | > hide #35 models |
| 4513 | | |
| 4514 | | > show #36 models |
| 4515 | | |
| 4516 | | > select add #36 |
| 4517 | | |
| 4518 | | 7214 atoms, 7373 bonds, 905 residues, 1 model selected |
| 4519 | | |
| 4520 | | > view matrix models #36,1,0,0,158.6,0,1,0,131.6,0,0,1,186.36 |
| 4521 | | |
| 4522 | | > view matrix models #36,1,0,0,112.24,0,1,0,235.54,0,0,1,195.51 |
| 4523 | | |
| 4524 | | > ui mousemode right "rotate selected models" |
| 4525 | | |
| 4526 | | > view matrix models |
| 4527 | | > #36,0.60674,-0.015574,0.79475,88.102,0.72366,0.42451,-0.54415,257.75,-0.3289,0.90529,0.26884,209.07 |
| 4528 | | |
| 4529 | | > ui mousemode right "translate selected models" |
| 4530 | | |
| 4531 | | > view matrix models |
| 4532 | | > #36,0.60674,-0.015574,0.79475,110.55,0.72366,0.42451,-0.54415,182.27,-0.3289,0.90529,0.26884,183.11 |
| 4533 | | |
| 4534 | | > view matrix models |
| 4535 | | > #36,0.60674,-0.015574,0.79475,107.23,0.72366,0.42451,-0.54415,189.41,-0.3289,0.90529,0.26884,154.84 |
| 4536 | | |
| 4537 | | > view matrix models |
| 4538 | | > #36,0.60674,-0.015574,0.79475,127.45,0.72366,0.42451,-0.54415,196.73,-0.3289,0.90529,0.26884,180.58 |
| 4539 | | |
| 4540 | | > fitmap #36 inMap #1 |
| 4541 | | |
| 4542 | | Fit molecule CopBprime_O55029.pdb (#36) to map |
| 4543 | | W20_J3623_consensus_structure_sharp.mrc (#1) using 7214 atoms |
| 4544 | | average map value = 0.2113, steps = 200 |
| 4545 | | shifted from previous position = 4.95 |
| 4546 | | rotated from previous position = 18 degrees |
| 4547 | | atoms outside contour = 5449, contour level = 0.26383 |
| 4548 | | |
| 4549 | | Position of CopBprime_O55029.pdb (#36) relative to |
| 4550 | | W20_J3623_consensus_structure_sharp.mrc (#1) coordinates: |
| 4551 | | Matrix rotation and translation |
| 4552 | | 0.50276875 0.12737660 0.85498467 126.31561890 |
| 4553 | | 0.61912593 0.63718187 -0.45900148 191.39912628 |
| 4554 | | -0.60324678 0.76011477 0.24149298 177.83802946 |
| 4555 | | Axis 0.62095632 0.74274954 0.25047230 |
| 4556 | | Axis point 74.13696267 0.00000000 87.94186273 |
| 4557 | | Rotation angle (degrees) 79.00508960 |
| 4558 | | Shift along axis 265.14159471 |
| 4559 | | |
| 4560 | | |
| 4561 | | > select subtract #36 |
| 4562 | | |
| 4563 | | Nothing selected |
| 4564 | | |
| 4565 | | > hide #36 models |
| 4566 | | |
| 4567 | | > show #37 models |
| 4568 | | |
| 4569 | | > select add #37 |
| 4570 | | |
| 4571 | | 4014 atoms, 4083 bonds, 511 residues, 1 model selected |
| 4572 | | |
| 4573 | | > view matrix models #37,1,0,0,30.593,0,1,0,199.33,0,0,1,198.85 |
| 4574 | | |
| 4575 | | > view matrix models #37,1,0,0,157.99,0,1,0,204.81,0,0,1,141.3 |
| 4576 | | |
| 4577 | | > ui mousemode right "rotate selected models" |
| 4578 | | |
| 4579 | | > view matrix models |
| 4580 | | > #37,0.43622,0.67922,-0.59023,141.13,-0.78114,-0.039782,-0.62309,191.16,-0.44669,0.73286,0.51321,126.99 |
| 4581 | | |
| 4582 | | > ui mousemode right "translate selected models" |
| 4583 | | |
| 4584 | | > view matrix models |
| 4585 | | > #37,0.43622,0.67922,-0.59023,150.33,-0.78114,-0.039782,-0.62309,186.91,-0.44669,0.73286,0.51321,121.12 |
| 4586 | | |
| 4587 | | > view matrix models |
| 4588 | | > #37,0.43622,0.67922,-0.59023,147.14,-0.78114,-0.039782,-0.62309,187.14,-0.44669,0.73286,0.51321,128.48 |
| 4589 | | |
| 4590 | | > fitmap #37 inMap #1 |
| 4591 | | |
| 4592 | | Fit molecule CopD_full_Q5XJY5.pdb (#37) to map |
| 4593 | | W20_J3623_consensus_structure_sharp.mrc (#1) using 4014 atoms |
| 4594 | | average map value = 0.1388, steps = 96 |
| 4595 | | shifted from previous position = 7.92 |
| 4596 | | rotated from previous position = 10.6 degrees |
| 4597 | | atoms outside contour = 3125, contour level = 0.26383 |
| 4598 | | |
| 4599 | | Position of CopD_full_Q5XJY5.pdb (#37) relative to |
| 4600 | | W20_J3623_consensus_structure_sharp.mrc (#1) coordinates: |
| 4601 | | Matrix rotation and translation |
| 4602 | | 0.41505472 0.79215751 -0.44745509 152.46783193 |
| 4603 | | -0.75869096 0.02993242 -0.65076269 180.94994695 |
| 4604 | | -0.50211314 0.60958226 0.61342633 132.05032423 |
| 4605 | | Axis 0.63044142 0.02734069 -0.77575518 |
| 4606 | | Axis point 250.00196774 -8.95488713 0.00000000 |
| 4607 | | Rotation angle (degrees) 88.32633929 |
| 4608 | | Shift along axis -1.36938916 |
| 4609 | | |
| 4610 | | |
| 4611 | | > view matrix models |
| 4612 | | > #37,0.41505,0.79216,-0.44746,143.76,-0.75869,0.029932,-0.65076,186.11,-0.50211,0.60958,0.61343,122.85 |
| 4613 | | |
| 4614 | | > view matrix models |
| 4615 | | > #37,0.41505,0.79216,-0.44746,143.43,-0.75869,0.029932,-0.65076,185.75,-0.50211,0.60958,0.61343,122.85 |
| 4616 | | |
| 4617 | | > view matrix models |
| 4618 | | > #37,0.41505,0.79216,-0.44746,142.4,-0.75869,0.029932,-0.65076,174.97,-0.50211,0.60958,0.61343,117.62 |
| 4619 | | |
| 4620 | | > fitmap #37 inMap #1 |
| 4621 | | |
| 4622 | | Fit molecule CopD_full_Q5XJY5.pdb (#37) to map |
| 4623 | | W20_J3623_consensus_structure_sharp.mrc (#1) using 4014 atoms |
| 4624 | | average map value = 0.1655, steps = 240 |
| 4625 | | shifted from previous position = 19.2 |
| 4626 | | rotated from previous position = 30 degrees |
| 4627 | | atoms outside contour = 2918, contour level = 0.26383 |
| 4628 | | |
| 4629 | | Position of CopD_full_Q5XJY5.pdb (#37) relative to |
| 4630 | | W20_J3623_consensus_structure_sharp.mrc (#1) coordinates: |
| 4631 | | Matrix rotation and translation |
| 4632 | | 0.54325309 0.50781797 -0.66857834 156.40844261 |
| 4633 | | -0.83919938 0.30481815 -0.45036685 164.83901629 |
| 4634 | | -0.02490957 0.80573371 0.59175392 110.35264351 |
| 4635 | | Axis 0.64381104 -0.32991074 -0.69041020 |
| 4636 | | Axis point 270.01565892 -103.01400891 0.00000000 |
| 4637 | | Rotation angle (degrees) 77.29610173 |
| 4638 | | Shift along axis -29.87327013 |
| 4639 | | |
| 4640 | | |
| 4641 | | > view matrix models |
| 4642 | | > #37,0.54325,0.50782,-0.66858,154.11,-0.8392,0.30482,-0.45037,160.13,-0.02491,0.80573,0.59175,113.09 |
| 4643 | | |
| 4644 | | > view matrix models |
| 4645 | | > #37,0.54325,0.50782,-0.66858,152.8,-0.8392,0.30482,-0.45037,160.99,-0.02491,0.80573,0.59175,116.7 |
| 4646 | | |
| 4647 | | > fitmap #37 inMap #1 |
| 4648 | | |
| 4649 | | Fit molecule CopD_full_Q5XJY5.pdb (#37) to map |
| 4650 | | W20_J3623_consensus_structure_sharp.mrc (#1) using 4014 atoms |
| 4651 | | average map value = 0.1527, steps = 200 |
| 4652 | | shifted from previous position = 8.47 |
| 4653 | | rotated from previous position = 9.78 degrees |
| 4654 | | atoms outside contour = 3000, contour level = 0.26383 |
| 4655 | | |
| 4656 | | Position of CopD_full_Q5XJY5.pdb (#37) relative to |
| 4657 | | W20_J3623_consensus_structure_sharp.mrc (#1) coordinates: |
| 4658 | | Matrix rotation and translation |
| 4659 | | 0.52381084 0.45458159 -0.72040113 159.66823341 |
| 4660 | | -0.84399300 0.16247112 -0.51115453 164.46346911 |
| 4661 | | -0.11531706 0.87576179 0.46876781 118.49109089 |
| 4662 | | Axis 0.69555148 -0.30345531 -0.65124728 |
| 4663 | | Axis point 0.00000000 33.10752297 257.99354223 |
| 4664 | | Rotation angle (degrees) 85.55368957 |
| 4665 | | Shift along axis -16.01683616 |
| 4666 | | |
| 4667 | | |
| 4668 | | > select subtract #37 |
| 4669 | | |
| 4670 | | Nothing selected |
| 4671 | | |
| 4672 | | > hide #37 models |
| 4673 | | |
| 4674 | | > show #38 models |
| 4675 | | |
| 4676 | | > select add #38 |
| 4677 | | |
| 4678 | | 6830 atoms, 6947 bonds, 874 residues, 1 model selected |
| 4679 | | |
| 4680 | | > view matrix models #38,1,0,0,4.9195,0,1,0,158.21,0,0,1,93.306 |
| 4681 | | |
| 4682 | | > view matrix models #38,1,0,0,20.598,0,1,0,103.1,0,0,1,122.07 |
| 4683 | | |
| 4684 | | > view matrix models #38,1,0,0,72.888,0,1,0,68.344,0,0,1,130.87 |
| 4685 | | |
| 4686 | | > view matrix models #38,1,0,0,89.688,0,1,0,109.93,0,0,1,127.2 |
| 4687 | | |
| 4688 | | > ui mousemode right "rotate selected models" |
| 4689 | | |
| 4690 | | > view matrix models |
| 4691 | | > #38,-0.10567,0.8283,0.55022,86.046,-0.72387,0.3153,-0.61367,108.49,-0.68179,-0.46314,0.56627,125.7 |
| 4692 | | |
| 4693 | | > ui mousemode right "translate selected models" |
| 4694 | | |
| 4695 | | > view matrix models |
| 4696 | | > #38,-0.10567,0.8283,0.55022,94.304,-0.72387,0.3153,-0.61367,138.65,-0.68179,-0.46314,0.56627,138.14 |
| 4697 | | |
| 4698 | | > view matrix models |
| 4699 | | > #38,-0.10567,0.8283,0.55022,112.86,-0.72387,0.3153,-0.61367,126.46,-0.68179,-0.46314,0.56627,130.13 |
| 4700 | | |
| 4701 | | > fitmap #38 inMap #1 |
| 4702 | | |
| 4703 | | Fit molecule CopG1_ Q9QZE5.pdb (#38) to map |
| 4704 | | W20_J3623_consensus_structure_sharp.mrc (#1) using 6830 atoms |
| 4705 | | average map value = 0.2013, steps = 104 |
| 4706 | | shifted from previous position = 5.21 |
| 4707 | | rotated from previous position = 10.3 degrees |
| 4708 | | atoms outside contour = 4944, contour level = 0.26383 |
| 4709 | | |
| 4710 | | Position of CopG1_ Q9QZE5.pdb (#38) relative to |
| 4711 | | W20_J3623_consensus_structure_sharp.mrc (#1) coordinates: |
| 4712 | | Matrix rotation and translation |
| 4713 | | -0.18152050 0.89700589 0.40302697 113.56564500 |
| 4714 | | -0.70329404 0.16803702 -0.69075398 122.94853762 |
| 4715 | | -0.68733384 -0.40883247 0.60035673 126.40848670 |
| 4716 | | Axis 0.14406783 0.55719735 -0.81778700 |
| 4717 | | Axis point 133.98876902 46.90995141 0.00000000 |
| 4718 | | Rotation angle (degrees) 101.92103286 |
| 4719 | | Shift along axis -18.50746083 |
| 4720 | | |
| 4721 | | |
| 4722 | | > select subtract #38 |
| 4723 | | |
| 4724 | | Nothing selected |
| 4725 | | |
| 4726 | | > hide #38 models |
| 4727 | | |
| 4728 | | > show #39 models |
| 4729 | | |
| 4730 | | > select add #39 |
| 4731 | | |
| 4732 | | 1420 atoms, 1441 bonds, 177 residues, 1 model selected |
| 4733 | | |
| 4734 | | > view matrix models #39,1,0,0,9.0898,0,1,0,160.45,0,0,1,-9.3444 |
| 4735 | | |
| 4736 | | > view matrix models #39,1,0,0,60.276,0,1,0,127.59,0,0,1,121.27 |
| 4737 | | |
| 4738 | | > view matrix models #39,1,0,0,127.7,0,1,0,116.45,0,0,1,94.356 |
| 4739 | | |
| 4740 | | > view matrix models #39,1,0,0,126.09,0,1,0,140.04,0,0,1,103.22 |
| 4741 | | |
| 4742 | | > ui mousemode right "rotate selected models" |
| 4743 | | |
| 4744 | | > view matrix models |
| 4745 | | > #39,0.77404,0.11317,-0.62294,121.61,-0.58601,0.50055,-0.63722,130.26,0.2397,0.85828,0.45376,105.69 |
| 4746 | | |
| 4747 | | > fitmap #39 inMap #1 |
| 4748 | | |
| 4749 | | Fit molecule CopZ1_P61924.pdb (#39) to map |
| 4750 | | W20_J3623_consensus_structure_sharp.mrc (#1) using 1420 atoms |
| 4751 | | average map value = 0.2019, steps = 236 |
| 4752 | | shifted from previous position = 4.91 |
| 4753 | | rotated from previous position = 38.4 degrees |
| 4754 | | atoms outside contour = 1084, contour level = 0.26383 |
| 4755 | | |
| 4756 | | Position of CopZ1_P61924.pdb (#39) relative to |
| 4757 | | W20_J3623_consensus_structure_sharp.mrc (#1) coordinates: |
| 4758 | | Matrix rotation and translation |
| 4759 | | 0.67082708 0.40413817 -0.62182263 120.91871935 |
| 4760 | | -0.65153203 -0.07936382 -0.75445836 130.14553587 |
| 4761 | | -0.35425563 0.91124845 0.21006956 98.85805425 |
| 4762 | | Axis 0.83698462 -0.13444710 -0.53045332 |
| 4763 | | Axis point 0.00000000 12.35504647 160.39862757 |
| 4764 | | Rotation angle (degrees) 95.69503877 |
| 4765 | | Shift along axis 31.26983397 |
| 4766 | | |
| 4767 | | |
| 4768 | | > select subtract #39 |
| 4769 | | |
| 4770 | | Nothing selected |
| 4771 | | |
| 4772 | | > hide #39 models |
| 4773 | | |
| 4774 | | > show #39 models |
| 4775 | | |
| 4776 | | > show #38 models |
| 4777 | | |
| 4778 | | > show #37 models |
| 4779 | | |
| 4780 | | > show #36 models |
| 4781 | | |
| 4782 | | > show #35 models |
| 4783 | | |
| 4784 | | > show #34 models |
| 4785 | | |
| 4786 | | > hide #!1 models |
| 4787 | | |
| 4788 | | > show #!3 models |
| 4789 | | |
| 4790 | | > fitmap #36 inMap #3 |
| 4791 | | |
| 4792 | | Fit molecule CopBprime_O55029.pdb (#36) to map |
| 4793 | | W20_J3985_004_volume_map_csparc_refine_of_relion_job121_bprime_mask.mrc (#3) |
| 4794 | | using 7214 atoms |
| 4795 | | average map value = 0.2322, steps = 64 |
| 4796 | | shifted from previous position = 2.88 |
| 4797 | | rotated from previous position = 4.24 degrees |
| 4798 | | atoms outside contour = 4591, contour level = 0.26875 |
| 4799 | | |
| 4800 | | Position of CopBprime_O55029.pdb (#36) relative to |
| 4801 | | W20_J3985_004_volume_map_csparc_refine_of_relion_job121_bprime_mask.mrc (#3) |
| 4802 | | coordinates: |
| 4803 | | Matrix rotation and translation |
| 4804 | | -0.39263805 -0.91469425 -0.09575906 92.65990042 |
| 4805 | | -0.55910845 0.32006970 -0.76482228 179.90077864 |
| 4806 | | 0.73022811 -0.24675864 -0.63708483 150.47739175 |
| 4807 | | Axis 0.49918086 -0.79588095 0.34262513 |
| 4808 | | Axis point 73.56777391 0.00000000 134.22819381 |
| 4809 | | Rotation angle (degrees) 148.74058258 |
| 4810 | | Shift along axis -45.36821860 |
| 4811 | | |
| 4812 | | |
| 4813 | | > fitmap #35 inMap #3 |
| 4814 | | |
| 4815 | | Fit molecule CopB_ Q9JIF7.pdb (#35) to map |
| 4816 | | W20_J3985_004_volume_map_csparc_refine_of_relion_job121_bprime_mask.mrc (#3) |
| 4817 | | using 7501 atoms |
| 4818 | | average map value = 0.2689, steps = 112 |
| 4819 | | shifted from previous position = 7.58 |
| 4820 | | rotated from previous position = 7.06 degrees |
| 4821 | | atoms outside contour = 3837, contour level = 0.26875 |
| 4822 | | |
| 4823 | | Position of CopB_ Q9JIF7.pdb (#35) relative to |
| 4824 | | W20_J3985_004_volume_map_csparc_refine_of_relion_job121_bprime_mask.mrc (#3) |
| 4825 | | coordinates: |
| 4826 | | Matrix rotation and translation |
| 4827 | | -0.23385104 0.28135848 0.93067238 134.35318178 |
| 4828 | | -0.41876188 -0.89302545 0.16475441 150.63397364 |
| 4829 | | 0.87746918 -0.35120213 0.32665714 138.57787474 |
| 4830 | | Axis -0.59215049 0.06105999 -0.80351072 |
| 4831 | | Axis point 39.18867412 76.76513978 0.00000000 |
| 4832 | | Rotation angle (degrees) 154.17248796 |
| 4833 | | Shift along axis -181.70840106 |
| 4834 | | |
| 4835 | | |
| 4836 | | > fitmap #34 inMap #3 |
| 4837 | | |
| 4838 | | Fit molecule CopA-F8WHL2.pdb (#34) to map |
| 4839 | | W20_J3985_004_volume_map_csparc_refine_of_relion_job121_bprime_mask.mrc (#3) |
| 4840 | | using 9810 atoms |
| 4841 | | average map value = 0.2333, steps = 88 |
| 4842 | | shifted from previous position = 0.574 |
| 4843 | | rotated from previous position = 1.73 degrees |
| 4844 | | atoms outside contour = 5294, contour level = 0.26875 |
| 4845 | | |
| 4846 | | Position of CopA-F8WHL2.pdb (#34) relative to |
| 4847 | | W20_J3985_004_volume_map_csparc_refine_of_relion_job121_bprime_mask.mrc (#3) |
| 4848 | | coordinates: |
| 4849 | | Matrix rotation and translation |
| 4850 | | 0.01921606 0.93572095 -0.35221737 156.63147150 |
| 4851 | | 0.36634751 0.32119202 0.87328416 124.11551889 |
| 4852 | | 0.93027967 -0.14581504 -0.33662699 137.77469414 |
| 4853 | | Axis -0.58763841 -0.73952029 -0.32831515 |
| 4854 | | Axis point 4.51722837 0.00000000 49.95590579 |
| 4855 | | Rotation angle (degrees) 119.87500102 |
| 4856 | | Shift along axis -229.06213308 |
| 4857 | | |
| 4858 | | |
| 4859 | | > hide #!3 models |
| 4860 | | |
| 4861 | | > show #!4 models |
| 4862 | | |
| 4863 | | > hide #!4 models |
| 4864 | | |
| 4865 | | > show #!5 models |
| 4866 | | |
| 4867 | | > hide #!5 models |
| 4868 | | |
| 4869 | | > show #!6 models |
| 4870 | | |
| 4871 | | > hide #!6 models |
| 4872 | | |
| 4873 | | > show #!7 models |
| 4874 | | |
| 4875 | | > hide #!7 models |
| 4876 | | |
| 4877 | | > show #!8 models |
| 4878 | | |
| 4879 | | > hide #!8 models |
| 4880 | | |
| 4881 | | > show #!9 models |
| 4882 | | |
| 4883 | | > fitmap #38 inMap #9 |
| 4884 | | |
| 4885 | | Fit molecule CopG1_ Q9QZE5.pdb (#38) to map |
| 4886 | | W20_J3988_004_volume_map_csparc_refine_of_relion_job126_gammazeta_mask.mrc |
| 4887 | | (#9) using 6830 atoms |
| 4888 | | average map value = 0.2056, steps = 112 |
| 4889 | | shifted from previous position = 3.87 |
| 4890 | | rotated from previous position = 8.6 degrees |
| 4891 | | atoms outside contour = 4655, contour level = 0.28232 |
| 4892 | | |
| 4893 | | Position of CopG1_ Q9QZE5.pdb (#38) relative to |
| 4894 | | W20_J3988_004_volume_map_csparc_refine_of_relion_job126_gammazeta_mask.mrc |
| 4895 | | (#9) coordinates: |
| 4896 | | Matrix rotation and translation |
| 4897 | | 0.97147165 -0.22568138 0.07287489 181.98815327 |
| 4898 | | -0.21631778 -0.96919397 -0.11776953 160.81365369 |
| 4899 | | 0.09720830 0.09864563 -0.99036336 147.52870008 |
| 4900 | | Axis 0.99282083 -0.11163133 0.04295621 |
| 4901 | | Axis point 0.00000000 86.77702657 74.77347983 |
| 4902 | | Rotation angle (degrees) 173.74290119 |
| 4903 | | Shift along axis 169.06706116 |
| 4904 | | |
| 4905 | | |
| 4906 | | > hide #!9 models |
| 4907 | | |
| 4908 | | > show #!10 models |
| 4909 | | |
| 4910 | | > hide #!10 models |
| 4911 | | |
| 4912 | | > show #!10 models |
| 4913 | | |
| 4914 | | > hide #!10 models |
| 4915 | | |
| 4916 | | > show #!10 models |
| 4917 | | |
| 4918 | | > hide #!10 models |
| 4919 | | |
| 4920 | | > show #!11 models |
| 4921 | | |
| 4922 | | > hide #!11 models |
| 4923 | | |
| 4924 | | > show #!23 models |
| 4925 | | |
| 4926 | | > fitmap #38 inMap #23 |
| 4927 | | |
| 4928 | | Fit molecule CopG1_ Q9QZE5.pdb (#38) to map |
| 4929 | | W20_J4026_004_volume_map_gammazeta_local.mrc (#23) using 6830 atoms |
| 4930 | | average map value = 0.1938, steps = 88 |
| 4931 | | shifted from previous position = 1.48 |
| 4932 | | rotated from previous position = 1.93 degrees |
| 4933 | | atoms outside contour = 4539, contour level = 0.26014 |
| 4934 | | |
| 4935 | | Position of CopG1_ Q9QZE5.pdb (#38) relative to |
| 4936 | | W20_J4026_004_volume_map_gammazeta_local.mrc (#23) coordinates: |
| 4937 | | Matrix rotation and translation |
| 4938 | | -0.15863519 0.95166183 0.26301070 116.52908933 |
| 4939 | | -0.70798986 0.07602756 -0.70211834 119.07331926 |
| 4940 | | -0.68817529 -0.29758959 0.66170629 125.26733072 |
| 4941 | | Axis 0.20689796 0.48648815 -0.84883597 |
| 4942 | | Axis point 135.80061352 37.60568602 0.00000000 |
| 4943 | | Rotation angle (degrees) 102.14876416 |
| 4944 | | Shift along axis -24.29402645 |
| 4945 | | |
| 4946 | | |
| 4947 | | > hide #!23 models |
| 4948 | | |
| 4949 | | > show #!25 models |
| 4950 | | |
| 4951 | | > fitmap #38 inMap #23 |
| 4952 | | |
| 4953 | | Fit molecule CopG1_ Q9QZE5.pdb (#38) to map |
| 4954 | | W20_J4026_004_volume_map_gammazeta_local.mrc (#23) using 6830 atoms |
| 4955 | | average map value = 0.1938, steps = 48 |
| 4956 | | shifted from previous position = 0.0265 |
| 4957 | | rotated from previous position = 0.0358 degrees |
| 4958 | | atoms outside contour = 4543, contour level = 0.26014 |
| 4959 | | |
| 4960 | | Position of CopG1_ Q9QZE5.pdb (#38) relative to |
| 4961 | | W20_J4026_004_volume_map_gammazeta_local.mrc (#23) coordinates: |
| 4962 | | Matrix rotation and translation |
| 4963 | | -0.15917554 0.95152929 0.26316374 116.52898909 |
| 4964 | | -0.70811196 0.07570421 -0.70203014 119.09089645 |
| 4965 | | -0.68792485 -0.29809542 0.66173902 125.28763210 |
| 4966 | | Axis 0.20661307 0.48648289 -0.84890838 |
| 4967 | | Axis point 135.76856788 37.64517816 0.00000000 |
| 4968 | | Rotation angle (degrees) 102.17311623 |
| 4969 | | Shift along axis -24.34562541 |
| 4970 | | |
| 4971 | | |
| 4972 | | > fitmap #38 inMap #25 |
| 4973 | | |
| 4974 | | Fit molecule CopG1_ Q9QZE5.pdb (#38) to map |
| 4975 | | W20_J4027_005_volume_map_gammazeta_local.mrc (#25) using 6830 atoms |
| 4976 | | average map value = 0.2208, steps = 312 |
| 4977 | | shifted from previous position = 9.89 |
| 4978 | | rotated from previous position = 43.3 degrees |
| 4979 | | atoms outside contour = 4003, contour level = 0.2532 |
| 4980 | | |
| 4981 | | Position of CopG1_ Q9QZE5.pdb (#38) relative to |
| 4982 | | W20_J4027_005_volume_map_gammazeta_local.mrc (#25) coordinates: |
| 4983 | | Matrix rotation and translation |
| 4984 | | -0.51349164 0.78944189 0.33631509 107.60498851 |
| 4985 | | -0.18358145 0.28179025 -0.94174949 122.78040261 |
| 4986 | | -0.83822681 -0.54532170 0.00022995 120.22835474 |
| 4987 | | Axis 0.25155556 0.74531240 -0.61743762 |
| 4988 | | Axis point 81.37402006 0.00000000 79.41692880 |
| 4989 | | Rotation angle (degrees) 128.00539846 |
| 4990 | | Shift along axis 44.34488087 |
| 4991 | | |
| 4992 | | |
| 4993 | | > hide #!25 models |
| 4994 | | |
| 4995 | | > show #!25 models |
| 4996 | | |
| 4997 | | > color #25 #929292ff models |
| 4998 | | |
| 4999 | | > color #25 #92929297 models |
| 5000 | | |
| 5001 | | > select add #39 |
| 5002 | | |
| 5003 | | 1420 atoms, 1441 bonds, 177 residues, 1 model selected |
| 5004 | | |
| 5005 | | > view matrix models |
| 5006 | | > #39,0.049821,0.95099,-0.30519,123.19,-0.98555,-0.0027194,-0.16936,132.83,-0.16189,0.30922,0.93711,101.07 |
| 5007 | | |
| 5008 | | > ui mousemode right "translate selected models" |
| 5009 | | |
| 5010 | | > view matrix models |
| 5011 | | > #39,0.049821,0.95099,-0.30519,120.81,-0.98555,-0.0027194,-0.16936,149.13,-0.16189,0.30922,0.93711,88.072 |
| 5012 | | |
| 5013 | | > view matrix models |
| 5014 | | > #39,0.049821,0.95099,-0.30519,127.16,-0.98555,-0.0027194,-0.16936,146.48,-0.16189,0.30922,0.93711,93.373 |
| 5015 | | |
| 5016 | | > fitmap #39 inMap #25 |
| 5017 | | |
| 5018 | | Fit molecule CopZ1_P61924.pdb (#39) to map |
| 5019 | | W20_J4027_005_volume_map_gammazeta_local.mrc (#25) using 1420 atoms |
| 5020 | | average map value = 0.2155, steps = 132 |
| 5021 | | shifted from previous position = 12.7 |
| 5022 | | rotated from previous position = 24.5 degrees |
| 5023 | | atoms outside contour = 816, contour level = 0.2532 |
| 5024 | | |
| 5025 | | Position of CopZ1_P61924.pdb (#39) relative to |
| 5026 | | W20_J4027_005_volume_map_gammazeta_local.mrc (#25) coordinates: |
| 5027 | | Matrix rotation and translation |
| 5028 | | -0.02172282 0.87569029 -0.48238434 117.17569269 |
| 5029 | | -0.85704432 -0.26475204 -0.44201968 143.50099331 |
| 5030 | | -0.51478457 0.40382284 0.75625654 98.49893470 |
| 5031 | | Axis 0.43861563 0.01680130 -0.89851770 |
| 5032 | | Axis point 143.73837501 16.52006781 0.00000000 |
| 5033 | | Rotation angle (degrees) 105.37343878 |
| 5034 | | Shift along axis -34.69694324 |
| 5035 | | |
| 5036 | | |
| 5037 | | > select subtract #39 |
| 5038 | | |
| 5039 | | Nothing selected |
| 5040 | | |
| 5041 | | > hide #39 models |
| 5042 | | |
| 5043 | | > hide #38 models |
| 5044 | | |
| 5045 | | > hide #37 models |
| 5046 | | |
| 5047 | | > hide #36 models |
| 5048 | | |
| 5049 | | > hide #35 models |
| 5050 | | |
| 5051 | | > show #35 models |
| 5052 | | |
| 5053 | | > hide #35 models |
| 5054 | | |
| 5055 | | > hide #34 models |
| 5056 | | |
| 5057 | | > show #34 models |
| 5058 | | |
| 5059 | | > show #35 models |
| 5060 | | |
| 5061 | | > show #36 models |
| 5062 | | |
| 5063 | | > show #37 models |
| 5064 | | |
| 5065 | | > show #38 models |
| 5066 | | |
| 5067 | | > show #39 models |
| 5068 | | |
| 5069 | | > open "/Users/becca/Desktop/Postdoc/COPI notebooks and resources/Alphafold |
| 5070 | | > predictions/fold_2025_01_17_alpha1_330gamma1_330zeta/fold_2025_01_17_alpha1_330gamma1_330zeta_model_0.cif" |
| 5071 | | |
| 5072 | | Chain information for fold_2025_01_17_alpha1_330gamma1_330zeta_model_0.cif #40 |
| 5073 | | --- |
| 5074 | | Chain | Description |
| 5075 | | A | . |
| 5076 | | B | . |
| 5077 | | C | . |
| 5078 | | |
| 5079 | | Computing secondary structure |
| 5080 | | |
| 5081 | | > select add #40 |
| 5082 | | |
| 5083 | | 6676 atoms, 6817 bonds, 837 residues, 1 model selected |
| 5084 | | |
| 5085 | | > ui tool show Matchmaker |
| 5086 | | |
| 5087 | | > matchmaker #40 to #34 |
| 5088 | | |
| 5089 | | Computing secondary structure |
| 5090 | | [Repeated 1 time(s)] Parameters |
| 5091 | | --- |
| 5092 | | Chain pairing | bb |
| 5093 | | Alignment algorithm | Needleman-Wunsch |
| 5094 | | Similarity matrix | BLOSUM-62 |
| 5095 | | SS fraction | 0.3 |
| 5096 | | Gap open (HH/SS/other) | 18/18/6 |
| 5097 | | Gap extend | 1 |
| 5098 | | SS matrix | | | H | S | O |
| 5099 | | ---|---|---|--- |
| 5100 | | H | 6 | -9 | -6 |
| 5101 | | S | | 6 | -6 |
| 5102 | | O | | | 4 |
| 5103 | | Iteration cutoff | 2 |
| 5104 | | |
| 5105 | | Matchmaker CopA-F8WHL2.pdb, chain A (#34) with |
| 5106 | | fold_2025_01_17_alpha1_330gamma1_330zeta_model_0.cif, chain B (#40), sequence |
| 5107 | | alignment score = 1723 |
| 5108 | | RMSD between 297 pruned atom pairs is 0.381 angstroms; (across all 330 pairs: |
| 5109 | | 4.425) |
| 5110 | | |
| 5111 | | |
| 5112 | | > select subtract #40 |
| 5113 | | |
| 5114 | | Nothing selected |
| 5115 | | |
| 5116 | | > fitmap #40 inMap #25 |
| 5117 | | |
| 5118 | | Fit molecule fold_2025_01_17_alpha1_330gamma1_330zeta_model_0.cif (#40) to map |
| 5119 | | W20_J4027_005_volume_map_gammazeta_local.mrc (#25) using 6676 atoms |
| 5120 | | average map value = 0.2327, steps = 120 |
| 5121 | | shifted from previous position = 12.5 |
| 5122 | | rotated from previous position = 19.1 degrees |
| 5123 | | atoms outside contour = 3778, contour level = 0.2532 |
| 5124 | | |
| 5125 | | Position of fold_2025_01_17_alpha1_330gamma1_330zeta_model_0.cif (#40) |
| 5126 | | relative to W20_J4027_005_volume_map_gammazeta_local.mrc (#25) coordinates: |
| 5127 | | Matrix rotation and translation |
| 5128 | | 0.54165871 0.66183244 -0.51825058 133.50428914 |
| 5129 | | 0.57871718 -0.74075578 -0.34112650 139.20476253 |
| 5130 | | -0.60966570 -0.11514637 -0.78425063 100.42690475 |
| 5131 | | Axis 0.87742311 0.35494156 -0.32271532 |
| 5132 | | Axis point 0.00000000 37.30416243 78.13952669 |
| 5133 | | Rotation angle (degrees) 172.60119629 |
| 5134 | | Shift along axis 134.14000391 |
| 5135 | | |
| 5136 | | |
| 5137 | | > ui tool show Matchmaker |
| 5138 | | |
| 5139 | | > matchmaker #39 to #40 |
| 5140 | | |
| 5141 | | Computing secondary structure |
| 5142 | | [Repeated 1 time(s)] Parameters |
| 5143 | | --- |
| 5144 | | Chain pairing | bb |
| 5145 | | Alignment algorithm | Needleman-Wunsch |
| 5146 | | Similarity matrix | BLOSUM-62 |
| 5147 | | SS fraction | 0.3 |
| 5148 | | Gap open (HH/SS/other) | 18/18/6 |
| 5149 | | Gap extend | 1 |
| 5150 | | SS matrix | | | H | S | O |
| 5151 | | ---|---|---|--- |
| 5152 | | H | 6 | -9 | -6 |
| 5153 | | S | | 6 | -6 |
| 5154 | | O | | | 4 |
| 5155 | | Iteration cutoff | 2 |
| 5156 | | |
| 5157 | | Matchmaker fold_2025_01_17_alpha1_330gamma1_330zeta_model_0.cif, chain C (#40) |
| 5158 | | with CopZ1_P61924.pdb, chain A (#39), sequence alignment score = 863.4 |
| 5159 | | RMSD between 141 pruned atom pairs is 0.407 angstroms; (across all 177 pairs: |
| 5160 | | 16.666) |
| 5161 | | |
| 5162 | | |
| 5163 | | > hide #40 models |
| 5164 | | |
| 5165 | | > fitmap #39 inMap #25 |
| 5166 | | |
| 5167 | | Fit molecule CopZ1_P61924.pdb (#39) to map |
| 5168 | | W20_J4027_005_volume_map_gammazeta_local.mrc (#25) using 1420 atoms |
| 5169 | | average map value = 0.237, steps = 448 |
| 5170 | | shifted from previous position = 13.1 |
| 5171 | | rotated from previous position = 65.5 degrees |
| 5172 | | atoms outside contour = 774, contour level = 0.2532 |
| 5173 | | |
| 5174 | | Position of CopZ1_P61924.pdb (#39) relative to |
| 5175 | | W20_J4027_005_volume_map_gammazeta_local.mrc (#25) coordinates: |
| 5176 | | Matrix rotation and translation |
| 5177 | | 0.40316931 0.69456576 0.59584639 114.25318729 |
| 5178 | | -0.23944244 -0.54836159 0.80122836 139.81166245 |
| 5179 | | 0.88324506 -0.46570160 -0.05477404 99.19539890 |
| 5180 | | Axis -0.79181872 -0.17962133 -0.58374591 |
| 5181 | | Axis point 0.00000000 56.29130506 -26.82207038 |
| 5182 | | Rotation angle (degrees) 126.86869176 |
| 5183 | | Shift along axis -173.48587819 |
| 5184 | | |
| 5185 | | |
| 5186 | | > undo |
| 5187 | | |
| 5188 | | > hide #39 models |
| 5189 | | |
| 5190 | | > hide #38 models |
| 5191 | | |
| 5192 | | > hide #37 models |
| 5193 | | |
| 5194 | | > hide #36 models |
| 5195 | | |
| 5196 | | > hide #35 models |
| 5197 | | |
| 5198 | | > hide #34 models |
| 5199 | | |
| 5200 | | > hide #!25 models |
| 5201 | | |
| 5202 | | > ui mousemode right select |
| 5203 | | |
| 5204 | | Drag select of 102 residues |
| 5205 | | |
| 5206 | | > select up |
| 5207 | | |
| 5208 | | 1089 atoms, 1111 bonds, 132 residues, 1 model selected |
| 5209 | | |
| 5210 | | > select up |
| 5211 | | |
| 5212 | | 2689 atoms, 2767 bonds, 330 residues, 1 model selected |
| 5213 | | |
| 5214 | | > delete sel |
| 5215 | | |
| 5216 | | > fitmap #40 inMap #25 |
| 5217 | | |
| 5218 | | Fit molecule fold_2025_01_17_alpha1_330gamma1_330zeta_model_0.cif (#40) to map |
| 5219 | | W20_J4027_005_volume_map_gammazeta_local.mrc (#25) using 3987 atoms |
| 5220 | | average map value = 0.2376, steps = 148 |
| 5221 | | shifted from previous position = 8.47 |
| 5222 | | rotated from previous position = 22.7 degrees |
| 5223 | | atoms outside contour = 2154, contour level = 0.2532 |
| 5224 | | |
| 5225 | | Position of fold_2025_01_17_alpha1_330gamma1_330zeta_model_0.cif (#40) |
| 5226 | | relative to W20_J4027_005_volume_map_gammazeta_local.mrc (#25) coordinates: |
| 5227 | | Matrix rotation and translation |
| 5228 | | 0.25729822 0.88581488 -0.38617305 122.95584280 |
| 5229 | | 0.78223190 -0.42556216 -0.45498364 149.35405458 |
| 5230 | | -0.56737191 -0.18501039 -0.80240904 100.43354571 |
| 5231 | | Axis 0.79114243 0.53099377 -0.30354451 |
| 5232 | | Axis point 0.00000000 25.55099714 77.70315234 |
| 5233 | | Rotation angle (degrees) 170.17599112 |
| 5234 | | Shift along axis 146.09560633 |
| 5235 | | |
| 5236 | | |
| 5237 | | > show #!25 models |
| 5238 | | |
| 5239 | | > ui tool show Matchmaker |
| 5240 | | |
| 5241 | | > matchmaker #39 to #40 |
| 5242 | | |
| 5243 | | Computing secondary structure |
| 5244 | | [Repeated 1 time(s)] Parameters |
| 5245 | | --- |
| 5246 | | Chain pairing | bb |
| 5247 | | Alignment algorithm | Needleman-Wunsch |
| 5248 | | Similarity matrix | BLOSUM-62 |
| 5249 | | SS fraction | 0.3 |
| 5250 | | Gap open (HH/SS/other) | 18/18/6 |
| 5251 | | Gap extend | 1 |
| 5252 | | SS matrix | | | H | S | O |
| 5253 | | ---|---|---|--- |
| 5254 | | H | 6 | -9 | -6 |
| 5255 | | S | | 6 | -6 |
| 5256 | | O | | | 4 |
| 5257 | | Iteration cutoff | 2 |
| 5258 | | |
| 5259 | | Matchmaker fold_2025_01_17_alpha1_330gamma1_330zeta_model_0.cif, chain C (#40) |
| 5260 | | with CopZ1_P61924.pdb, chain A (#39), sequence alignment score = 863.4 |
| 5261 | | RMSD between 141 pruned atom pairs is 0.407 angstroms; (across all 177 pairs: |
| 5262 | | 16.666) |
| 5263 | | |
| 5264 | | |
| 5265 | | > show #39 models |
| 5266 | | |
| 5267 | | > hide #40 models |
| 5268 | | |
| 5269 | | > show #38 models |
| 5270 | | |
| 5271 | | > show #34 models |
| 5272 | | |
| 5273 | | > hide #39 models |
| 5274 | | |
| 5275 | | > hide #38 models |
| 5276 | | |
| 5277 | | > hide #!25 models |
| 5278 | | |
| 5279 | | Drag select of 84 residues |
| 5280 | | |
| 5281 | | > select up |
| 5282 | | |
| 5283 | | 956 atoms, 971 bonds, 118 residues, 1 model selected |
| 5284 | | |
| 5285 | | > select up |
| 5286 | | |
| 5287 | | 9810 atoms, 10032 bonds, 1233 residues, 1 model selected |
| 5288 | | |
| 5289 | | > select down |
| 5290 | | |
| 5291 | | 956 atoms, 971 bonds, 118 residues, 1 model selected |
| 5292 | | Drag select of 309 residues |
| 5293 | | |
| 5294 | | > delete sel |
| 5295 | | |
| 5296 | | > select clear |
| 5297 | | |
| 5298 | | [Repeated 2 time(s)]Drag select of 1 pseudobonds |
| 5299 | | |
| 5300 | | > delete sel |
| 5301 | | |
| 5302 | | > select add #34 |
| 5303 | | |
| 5304 | | 7345 atoms, 7512 bonds, 2 pseudobonds, 924 residues, 2 models selected |
| 5305 | | |
| 5306 | | > select subtract #34 |
| 5307 | | |
| 5308 | | Nothing selected |
| 5309 | | |
| 5310 | | > delete sel |
| 5311 | | |
| 5312 | | > close #34 |
| 5313 | | |
| 5314 | | > open "/Users/becca/Desktop/Postdoc/COPI notebooks and resources/Structure |
| 5315 | | > files/alphafold/CopA-F8WHL2.pdb" |
| 5316 | | |
| 5317 | | CopA-F8WHL2.pdb title: |
| 5318 | | Alphafold monomer V2.0 prediction for coatomer subunit α (F8WHL2) [more |
| 5319 | | info...] |
| 5320 | | |
| 5321 | | Chain information for CopA-F8WHL2.pdb #34 |
| 5322 | | --- |
| 5323 | | Chain | Description | UniProt |
| 5324 | | A | coatomer subunit α | F8WHL2_MOUSE 1-1233 |
| 5325 | | |
| 5326 | | Computing secondary structure |
| 5327 | | |
| 5328 | | > show #!25 models |
| 5329 | | |
| 5330 | | > select add #34 |
| 5331 | | |
| 5332 | | 9810 atoms, 10032 bonds, 1233 residues, 1 model selected |
| 5333 | | |
| 5334 | | > color sel navy |
| 5335 | | |
| 5336 | | > ui mousemode right "translate selected models" |
| 5337 | | |
| 5338 | | > view matrix models #34,1,0,0,139.38,0,1,0,77.792,0,0,1,174.15 |
| 5339 | | |
| 5340 | | > view matrix models #34,1,0,0,143.17,0,1,0,116.78,0,0,1,157.74 |
| 5341 | | |
| 5342 | | > ui mousemode right "rotate selected models" |
| 5343 | | |
| 5344 | | > view matrix models |
| 5345 | | > #34,-0.77292,0.46571,-0.43094,110.33,-0.63189,-0.50329,0.58942,111.94,0.057612,0.72788,0.68328,155.75 |
| 5346 | | |
| 5347 | | > view matrix models |
| 5348 | | > #34,0.095488,0.54565,-0.83256,128.67,-0.72338,-0.53652,-0.43459,118.09,-0.68382,0.64375,0.34348,145.26 |
| 5349 | | |
| 5350 | | > view matrix models |
| 5351 | | > #34,0.86645,-0.32341,0.38035,140.29,0.26177,0.943,0.20551,120.49,-0.42513,-0.078497,0.90172,151.23 |
| 5352 | | |
| 5353 | | > view matrix models |
| 5354 | | > #34,-0.13249,-0.98099,-0.14179,130.68,-0.29114,-0.098228,0.95162,112.52,-0.94746,0.16737,-0.27259,148.51 |
| 5355 | | |
| 5356 | | > view matrix models |
| 5357 | | > #34,-0.191,-0.9337,-0.30287,130.44,-0.10946,-0.28637,0.95185,117.25,-0.97547,0.21495,-0.047502,145.98 |
| 5358 | | |
| 5359 | | > view matrix models |
| 5360 | | > #34,-0.60871,-0.63739,-0.47244,121.83,0.33431,-0.74608,0.57585,131.6,-0.71952,0.19258,0.66723,145.55 |
| 5361 | | |
| 5362 | | > ui mousemode right "translate selected models" |
| 5363 | | |
| 5364 | | > view matrix models |
| 5365 | | > #34,-0.60871,-0.63739,-0.47244,153.15,0.33431,-0.74608,0.57585,151.65,-0.71952,0.19258,0.66723,153.4 |
| 5366 | | |
| 5367 | | > view matrix models |
| 5368 | | > #34,-0.60871,-0.63739,-0.47244,154.56,0.33431,-0.74608,0.57585,133.83,-0.71952,0.19258,0.66723,140.34 |
| 5369 | | |
| 5370 | | > ui mousemode right "rotate selected models" |
| 5371 | | |
| 5372 | | > view matrix models |
| 5373 | | > #34,-0.61566,-0.09401,-0.78238,152.7,0.38273,-0.90356,-0.1926,141.58,-0.68882,-0.41802,0.59227,145.99 |
| 5374 | | |
| 5375 | | > ui mousemode right "translate selected models" |
| 5376 | | |
| 5377 | | > view matrix models |
| 5378 | | > #34,-0.61566,-0.09401,-0.78238,154.2,0.38273,-0.90356,-0.1926,117.01,-0.68882,-0.41802,0.59227,135.94 |
| 5379 | | |
| 5380 | | > select up |
| 5381 | | |
| 5382 | | 40776 atoms, 41545 bonds, 5160 residues, 68 models selected |
| 5383 | | |
| 5384 | | > select down |
| 5385 | | |
| 5386 | | 9810 atoms, 10032 bonds, 1233 residues, 1 model selected |
| 5387 | | |
| 5388 | | > ui mousemode right select |
| 5389 | | |
| 5390 | | > select clear |
| 5391 | | |
| 5392 | | > fitmap #34 inMap #25 |
| 5393 | | |
| 5394 | | Fit molecule CopA-F8WHL2.pdb (#34) to map |
| 5395 | | W20_J4027_005_volume_map_gammazeta_local.mrc (#25) using 9810 atoms |
| 5396 | | average map value = 0.2513, steps = 268 |
| 5397 | | shifted from previous position = 12.5 |
| 5398 | | rotated from previous position = 28.3 degrees |
| 5399 | | atoms outside contour = 4976, contour level = 0.2532 |
| 5400 | | |
| 5401 | | Position of CopA-F8WHL2.pdb (#34) relative to |
| 5402 | | W20_J4027_005_volume_map_gammazeta_local.mrc (#25) coordinates: |
| 5403 | | Matrix rotation and translation |
| 5404 | | -0.53264139 -0.54606136 -0.64661437 159.40933932 |
| 5405 | | 0.58383331 -0.79018975 0.18638357 127.28571811 |
| 5406 | | -0.61272491 -0.27823941 0.73969657 133.57411156 |
| 5407 | | Axis -0.38016417 -0.02772906 0.92450327 |
| 5408 | | Axis point 83.38396978 99.21518793 0.00000000 |
| 5409 | | Rotation angle (degrees) 142.33221845 |
| 5410 | | Shift along axis 59.35847140 |
| 5411 | | |
| 5412 | | |
| 5413 | | > hide #!25 models |
| 5414 | | |
| 5415 | | Drag select of 204 residues |
| 5416 | | |
| 5417 | | > select up |
| 5418 | | |
| 5419 | | 1872 atoms, 1904 bonds, 233 residues, 1 model selected |
| 5420 | | |
| 5421 | | > delete sel |
| 5422 | | |
| 5423 | | Drag select of 67 residues, 2 pseudobonds |
| 5424 | | Drag select of 44 residues, 3 pseudobonds |
| 5425 | | |
| 5426 | | > select up |
| 5427 | | |
| 5428 | | 515 atoms, 525 bonds, 3 pseudobonds, 65 residues, 2 models selected |
| 5429 | | |
| 5430 | | > select up |
| 5431 | | |
| 5432 | | 7938 atoms, 8122 bonds, 3 pseudobonds, 1000 residues, 2 models selected |
| 5433 | | |
| 5434 | | > select down |
| 5435 | | |
| 5436 | | 515 atoms, 525 bonds, 3 pseudobonds, 65 residues, 2 models selected |
| 5437 | | Drag select of 62 residues, 3 pseudobonds |
| 5438 | | |
| 5439 | | > select up |
| 5440 | | |
| 5441 | | 597 atoms, 611 bonds, 3 pseudobonds, 76 residues, 2 models selected |
| 5442 | | |
| 5443 | | > delete sel |
| 5444 | | |
| 5445 | | Drag select of 2 residues |
| 5446 | | |
| 5447 | | > select up |
| 5448 | | |
| 5449 | | 645 atoms, 657 bonds, 90 residues, 1 model selected |
| 5450 | | |
| 5451 | | > delete sel |
| 5452 | | |
| 5453 | | > show #!25 models |
| 5454 | | |
| 5455 | | > fitmap #34 inMap #25 |
| 5456 | | |
| 5457 | | Fit molecule CopA-F8WHL2.pdb (#34) to map |
| 5458 | | W20_J4027_005_volume_map_gammazeta_local.mrc (#25) using 6696 atoms |
| 5459 | | average map value = 0.33, steps = 92 |
| 5460 | | shifted from previous position = 0.363 |
| 5461 | | rotated from previous position = 1.55 degrees |
| 5462 | | atoms outside contour = 2309, contour level = 0.2532 |
| 5463 | | |
| 5464 | | Position of CopA-F8WHL2.pdb (#34) relative to |
| 5465 | | W20_J4027_005_volume_map_gammazeta_local.mrc (#25) coordinates: |
| 5466 | | Matrix rotation and translation |
| 5467 | | -0.55114104 -0.52745699 -0.64655447 159.68929082 |
| 5468 | | 0.57420657 -0.80195943 0.16476617 126.61771889 |
| 5469 | | -0.60541752 -0.28044643 0.74486202 133.68887023 |
| 5470 | | Axis -0.37446301 -0.03459979 0.92659609 |
| 5471 | | Axis point 83.97786314 98.17305376 0.00000000 |
| 5472 | | Rotation angle (degrees) 143.52527944 |
| 5473 | | Shift along axis 59.69690535 |
| 5474 | | |
| 5475 | | |
| 5476 | | > hide #!34 models |
| 5477 | | |
| 5478 | | > show #35 models |
| 5479 | | |
| 5480 | | > fitmap #35 inMap #25 |
| 5481 | | |
| 5482 | | Fit molecule CopB_ Q9JIF7.pdb (#35) to map |
| 5483 | | W20_J4027_005_volume_map_gammazeta_local.mrc (#25) using 7501 atoms |
| 5484 | | average map value = 0.2378, steps = 84 |
| 5485 | | shifted from previous position = 1.34 |
| 5486 | | rotated from previous position = 3.2 degrees |
| 5487 | | atoms outside contour = 4024, contour level = 0.2532 |
| 5488 | | |
| 5489 | | Position of CopB_ Q9JIF7.pdb (#35) relative to |
| 5490 | | W20_J4027_005_volume_map_gammazeta_local.mrc (#25) coordinates: |
| 5491 | | Matrix rotation and translation |
| 5492 | | 0.32064619 0.78921696 -0.52375815 142.21372577 |
| 5493 | | 0.68088169 -0.57645114 -0.45177892 148.04005904 |
| 5494 | | -0.65847257 -0.21175615 -0.72220024 154.69062564 |
| 5495 | | Axis 0.81144623 0.45542974 -0.36624960 |
| 5496 | | Axis point 0.00000000 24.90601335 113.58992692 |
| 5497 | | Rotation angle (degrees) 171.49485982 |
| 5498 | | Shift along axis 126.16525710 |
| 5499 | | |
| 5500 | | |
| 5501 | | > fitmap #35 inMap #25 |
| 5502 | | |
| 5503 | | Fit molecule CopB_ Q9JIF7.pdb (#35) to map |
| 5504 | | W20_J4027_005_volume_map_gammazeta_local.mrc (#25) using 7501 atoms |
| 5505 | | average map value = 0.2378, steps = 48 |
| 5506 | | shifted from previous position = 0.00712 |
| 5507 | | rotated from previous position = 0.00209 degrees |
| 5508 | | atoms outside contour = 4022, contour level = 0.2532 |
| 5509 | | |
| 5510 | | Position of CopB_ Q9JIF7.pdb (#35) relative to |
| 5511 | | W20_J4027_005_volume_map_gammazeta_local.mrc (#25) coordinates: |
| 5512 | | Matrix rotation and translation |
| 5513 | | 0.32064133 0.78920396 -0.52378071 142.21243830 |
| 5514 | | 0.68089354 -0.57645720 -0.45175334 148.03333799 |
| 5515 | | -0.65846269 -0.21178811 -0.72219988 154.68860774 |
| 5516 | | Axis 0.81144528 0.45542871 -0.36625298 |
| 5517 | | Axis point 0.00000000 24.90541120 113.58774992 |
| 5518 | | Rotation angle (degrees) 171.49690419 |
| 5519 | | Shift along axis 126.16107988 |
| 5520 | | |
| 5521 | | |
| 5522 | | > hide #35 models |
| 5523 | | |
| 5524 | | > show #36 models |
| 5525 | | |
| 5526 | | > fitmap #36 inMap #25 |
| 5527 | | |
| 5528 | | Fit molecule CopBprime_O55029.pdb (#36) to map |
| 5529 | | W20_J4027_005_volume_map_gammazeta_local.mrc (#25) using 7214 atoms |
| 5530 | | average map value = 0.1828, steps = 80 |
| 5531 | | shifted from previous position = 1.71 |
| 5532 | | rotated from previous position = 0.68 degrees |
| 5533 | | atoms outside contour = 6397, contour level = 0.2532 |
| 5534 | | |
| 5535 | | Position of CopBprime_O55029.pdb (#36) relative to |
| 5536 | | W20_J4027_005_volume_map_gammazeta_local.mrc (#25) coordinates: |
| 5537 | | Matrix rotation and translation |
| 5538 | | 0.50825278 0.05183494 0.85964658 125.87415961 |
| 5539 | | 0.66191789 0.61506689 -0.42843603 193.30517521 |
| 5540 | | -0.55094811 0.78676926 0.27829898 176.59370632 |
| 5541 | | Axis 0.62023665 0.71996274 0.31138426 |
| 5542 | | Axis point 57.84080416 0.00000000 83.07791765 |
| 5543 | | Rotation angle (degrees) 78.41570987 |
| 5544 | | Shift along axis 272.23279077 |
| 5545 | | |
| 5546 | | |
| 5547 | | > hide #36 models |
| 5548 | | |
| 5549 | | > show #36 models |
| 5550 | | |
| 5551 | | > hide #36 models |
| 5552 | | |
| 5553 | | > show #37 models |
| 5554 | | |
| 5555 | | > hide #!25 models |
| 5556 | | |
| 5557 | | Drag select of 74 residues |
| 5558 | | |
| 5559 | | > select up |
| 5560 | | |
| 5561 | | 696 atoms, 711 bonds, 90 residues, 1 model selected |
| 5562 | | |
| 5563 | | > delete sel |
| 5564 | | |
| 5565 | | > select clear |
| 5566 | | |
| 5567 | | Drag select of 39 residues |
| 5568 | | |
| 5569 | | > select up |
| 5570 | | |
| 5571 | | 652 atoms, 661 bonds, 80 residues, 1 model selected |
| 5572 | | |
| 5573 | | > delete sel |
| 5574 | | |
| 5575 | | Drag select of 72 residues, 2 pseudobonds |
| 5576 | | |
| 5577 | | > delete sel |
| 5578 | | |
| 5579 | | > show #!25 models |
| 5580 | | |
| 5581 | | > select add #25 |
| 5582 | | |
| 5583 | | 2 models selected |
| 5584 | | |
| 5585 | | > select subtract #25 |
| 5586 | | |
| 5587 | | Nothing selected |
| 5588 | | |
| 5589 | | > select add #37 |
| 5590 | | |
| 5591 | | 2098 atoms, 2129 bonds, 269 residues, 1 model selected |
| 5592 | | Drag select of 25 W20_J4027_005_volume_map_gammazeta_local.mrc , 10 residues |
| 5593 | | |
| 5594 | | > select clear |
| 5595 | | |
| 5596 | | > select add #37 |
| 5597 | | |
| 5598 | | 2098 atoms, 2129 bonds, 269 residues, 1 model selected |
| 5599 | | |
| 5600 | | > ui mousemode right "translate selected models" |
| 5601 | | |
| 5602 | | > view matrix models |
| 5603 | | > #37,0.52381,0.45458,-0.7204,159.74,-0.84399,0.16247,-0.51115,164.47,-0.11532,0.87576,0.46877,118.38 |
| 5604 | | |
| 5605 | | > view matrix models |
| 5606 | | > #37,0.52381,0.45458,-0.7204,159.87,-0.84399,0.16247,-0.51115,160.32,-0.11532,0.87576,0.46877,115.66 |
| 5607 | | |
| 5608 | | > ui mousemode right "rotate selected models" |
| 5609 | | |
| 5610 | | > view matrix models |
| 5611 | | > #37,-0.51843,-0.78578,-0.33732,156.47,0.64386,-0.0991,-0.7587,177.15,0.56274,-0.61052,0.55731,131.39 |
| 5612 | | |
| 5613 | | > ui mousemode right "translate selected models" |
| 5614 | | |
| 5615 | | > view matrix models |
| 5616 | | > #37,-0.51843,-0.78578,-0.33732,156.82,0.64386,-0.0991,-0.7587,177.21,0.56274,-0.61052,0.55731,131.11 |
| 5617 | | |
| 5618 | | > view matrix models |
| 5619 | | > #37,-0.51843,-0.78578,-0.33732,169.14,0.64386,-0.0991,-0.7587,188.43,0.56274,-0.61052,0.55731,154.61 |
| 5620 | | |
| 5621 | | > view matrix models |
| 5622 | | > #37,-0.51843,-0.78578,-0.33732,168.55,0.64386,-0.0991,-0.7587,185.71,0.56274,-0.61052,0.55731,156.17 |
| 5623 | | |
| 5624 | | > fitmap #37 inMap #25 |
| 5625 | | |
| 5626 | | Fit molecule CopD_full_Q5XJY5.pdb (#37) to map |
| 5627 | | W20_J4027_005_volume_map_gammazeta_local.mrc (#25) using 2098 atoms |
| 5628 | | average map value = 0.1523, steps = 264 |
| 5629 | | shifted from previous position = 11.8 |
| 5630 | | rotated from previous position = 42.5 degrees |
| 5631 | | atoms outside contour = 1552, contour level = 0.2532 |
| 5632 | | |
| 5633 | | Position of CopD_full_Q5XJY5.pdb (#37) relative to |
| 5634 | | W20_J4027_005_volume_map_gammazeta_local.mrc (#25) coordinates: |
| 5635 | | Matrix rotation and translation |
| 5636 | | 0.13421510 -0.97879473 -0.15474878 179.22768371 |
| 5637 | | 0.80299796 0.19893091 -0.56180136 174.64912074 |
| 5638 | | 0.58067252 -0.04886073 0.81266971 148.66115690 |
| 5639 | | Axis 0.25715468 -0.36869186 0.89327363 |
| 5640 | | Axis point -62.36776041 208.19731599 0.00000000 |
| 5641 | | Rotation angle (degrees) 85.81897760 |
| 5642 | | Shift along axis 114.49262034 |
| 5643 | | |
| 5644 | | |
| 5645 | | > ui mousemode right "rotate selected models" |
| 5646 | | |
| 5647 | | > view matrix models |
| 5648 | | > #37,0.17652,-0.98345,-0.040823,181.47,0.73295,0.15901,-0.66143,172.16,0.65698,0.086834,0.74889,148.4 |
| 5649 | | |
| 5650 | | > view matrix models |
| 5651 | | > #37,0.4517,-0.88251,-0.13091,183.25,0.50738,0.3748,-0.77595,166,0.73385,0.28407,0.61707,146.24 |
| 5652 | | |
| 5653 | | > view matrix models |
| 5654 | | > #37,0.19394,-0.76059,-0.61959,169.31,0.33983,0.64457,-0.68487,164.7,0.92028,-0.077732,0.38347,145.18 |
| 5655 | | |
| 5656 | | > ui mousemode right "translate selected models" |
| 5657 | | |
| 5658 | | > ui mousemode right "rotate selected models" |
| 5659 | | |
| 5660 | | > view matrix models |
| 5661 | | > #37,0.13058,-0.95382,-0.27052,176.06,0.41699,0.30039,-0.85784,163.27,0.89948,-0.00078766,0.43696,145.75 |
| 5662 | | |
| 5663 | | > ui mousemode right "translate selected models" |
| 5664 | | |
| 5665 | | > view matrix models |
| 5666 | | > #37,0.13058,-0.95382,-0.27052,175.12,0.41699,0.30039,-0.85784,169.65,0.89948,-0.00078766,0.43696,144.2 |
| 5667 | | |
| 5668 | | > view matrix models |
| 5669 | | > #37,0.13058,-0.95382,-0.27052,176.92,0.41699,0.30039,-0.85784,173.28,0.89948,-0.00078766,0.43696,142.71 |
| 5670 | | |
| 5671 | | > view matrix models |
| 5672 | | > #37,0.13058,-0.95382,-0.27052,176.07,0.41699,0.30039,-0.85784,171.32,0.89948,-0.00078766,0.43696,141.37 |
| 5673 | | |
| 5674 | | > ui mousemode right "rotate selected models" |
| 5675 | | |
| 5676 | | > view matrix models |
| 5677 | | > #37,0.98025,0.13893,0.14075,193.31,0.13603,0.042901,-0.98977,165.4,-0.14355,0.98937,0.023155,115.29 |
| 5678 | | |
| 5679 | | > view matrix models |
| 5680 | | > #37,-0.70422,-0.31127,-0.63811,154.89,0.39637,-0.91803,0.010376,192.21,-0.58904,-0.24562,0.76987,127.74 |
| 5681 | | |
| 5682 | | > view matrix models |
| 5683 | | > #37,-0.068744,0.46239,-0.88401,156.66,-0.96301,-0.26218,-0.062249,169.55,-0.26056,0.84703,0.46331,123 |
| 5684 | | |
| 5685 | | > ui mousemode right "translate selected models" |
| 5686 | | |
| 5687 | | > view matrix models |
| 5688 | | > #37,-0.068744,0.46239,-0.88401,155.46,-0.96301,-0.26218,-0.062249,163.71,-0.26056,0.84703,0.46331,114.14 |
| 5689 | | |
| 5690 | | > view matrix models |
| 5691 | | > #37,-0.068744,0.46239,-0.88401,155.61,-0.96301,-0.26218,-0.062249,167.76,-0.26056,0.84703,0.46331,108.27 |
| 5692 | | |
| 5693 | | > view matrix models |
| 5694 | | > #37,-0.068744,0.46239,-0.88401,151.7,-0.96301,-0.26218,-0.062249,167.34,-0.26056,0.84703,0.46331,109.96 |
| 5695 | | |
| 5696 | | > view matrix models |
| 5697 | | > #37,-0.068744,0.46239,-0.88401,148.05,-0.96301,-0.26218,-0.062249,168.6,-0.26056,0.84703,0.46331,113.26 |
| 5698 | | |
| 5699 | | > fitmap #37 inMap #25 |
| 5700 | | |
| 5701 | | Fit molecule CopD_full_Q5XJY5.pdb (#37) to map |
| 5702 | | W20_J4027_005_volume_map_gammazeta_local.mrc (#25) using 2098 atoms |
| 5703 | | average map value = 0.188, steps = 268 |
| 5704 | | shifted from previous position = 1.8 |
| 5705 | | rotated from previous position = 31.7 degrees |
| 5706 | | atoms outside contour = 1411, contour level = 0.2532 |
| 5707 | | |
| 5708 | | Position of CopD_full_Q5XJY5.pdb (#37) relative to |
| 5709 | | W20_J4027_005_volume_map_gammazeta_local.mrc (#25) coordinates: |
| 5710 | | Matrix rotation and translation |
| 5711 | | -0.10900924 0.75139291 -0.65078850 146.28424526 |
| 5712 | | -0.97991274 0.02875802 0.19734233 168.20699465 |
| 5713 | | 0.16699701 0.65922808 0.73316460 122.79115227 |
| 5714 | | Axis 0.23450114 -0.41519279 -0.87899042 |
| 5715 | | Axis point 141.38224991 -30.78701025 0.00000000 |
| 5716 | | Rotation angle (degrees) 99.99389905 |
| 5717 | | Shift along axis -143.46675599 |
| 5718 | | |
| 5719 | | |
| 5720 | | > select subtract #37 |
| 5721 | | |
| 5722 | | Nothing selected |
| 5723 | | |
| 5724 | | > hide #!37 models |
| 5725 | | |
| 5726 | | > open "/Users/becca/Desktop/Postdoc/COPI notebooks and resources/Alphafold |
| 5727 | | > predictions/alphafold 3 |
| 5728 | | > /fold_20240724_copbdg1z1_try2/fold_20240724_copbdg1z1_try2_model_0.cif" |
| 5729 | | |
| 5730 | | Chain information for fold_20240724_copbdg1z1_try2_model_0.cif #41 |
| 5731 | | --- |
| 5732 | | Chain | Description |
| 5733 | | A | . |
| 5734 | | B | . |
| 5735 | | C | . |
| 5736 | | D | . |
| 5737 | | |
| 5738 | | Computing secondary structure |
| 5739 | | |
| 5740 | | > select add #41 |
| 5741 | | |
| 5742 | | 19765 atoms, 20090 bonds, 2515 residues, 1 model selected |
| 5743 | | |
| 5744 | | > ui tool show Matchmaker |
| 5745 | | |
| 5746 | | > matchmaker #41 to #35 |
| 5747 | | |
| 5748 | | Computing secondary structure |
| 5749 | | [Repeated 1 time(s)] Parameters |
| 5750 | | --- |
| 5751 | | Chain pairing | bb |
| 5752 | | Alignment algorithm | Needleman-Wunsch |
| 5753 | | Similarity matrix | BLOSUM-62 |
| 5754 | | SS fraction | 0.3 |
| 5755 | | Gap open (HH/SS/other) | 18/18/6 |
| 5756 | | Gap extend | 1 |
| 5757 | | SS matrix | | | H | S | O |
| 5758 | | ---|---|---|--- |
| 5759 | | H | 6 | -9 | -6 |
| 5760 | | S | | 6 | -6 |
| 5761 | | O | | | 4 |
| 5762 | | Iteration cutoff | 2 |
| 5763 | | |
| 5764 | | Matchmaker CopB_ Q9JIF7.pdb, chain A (#35) with |
| 5765 | | fold_20240724_copbdg1z1_try2_model_0.cif, chain A (#41), sequence alignment |
| 5766 | | score = 4801.7 |
| 5767 | | RMSD between 351 pruned atom pairs is 0.825 angstroms; (across all 953 pairs: |
| 5768 | | 12.162) |
| 5769 | | |
| 5770 | | |
| 5771 | | > fitmap #41 inMap #25 |
| 5772 | | |
| 5773 | | Fit molecule fold_20240724_copbdg1z1_try2_model_0.cif (#41) to map |
| 5774 | | W20_J4027_005_volume_map_gammazeta_local.mrc (#25) using 19765 atoms |
| 5775 | | average map value = 0.1792, steps = 108 |
| 5776 | | shifted from previous position = 5.04 |
| 5777 | | rotated from previous position = 8.73 degrees |
| 5778 | | atoms outside contour = 12927, contour level = 0.2532 |
| 5779 | | |
| 5780 | | Position of fold_20240724_copbdg1z1_try2_model_0.cif (#41) relative to |
| 5781 | | W20_J4027_005_volume_map_gammazeta_local.mrc (#25) coordinates: |
| 5782 | | Matrix rotation and translation |
| 5783 | | 0.76300462 0.02891891 -0.64574581 129.45795402 |
| 5784 | | 0.59375195 -0.42623762 0.68248085 141.01253356 |
| 5785 | | -0.25550456 -0.90414887 -0.34239193 134.21937577 |
| 5786 | | Axis -0.91776836 -0.22573072 0.32672141 |
| 5787 | | Axis point 0.00000000 107.57830339 53.51898785 |
| 5788 | | Rotation angle (degrees) 120.18624651 |
| 5789 | | Shift along axis -106.79093220 |
| 5790 | | |
| 5791 | | |
| 5792 | | > hide #!25 models |
| 5793 | | |
| 5794 | | > view matrix models |
| 5795 | | > #41,0.75987,0.021282,-0.64972,128.96,0.5945,-0.42707,0.6813,141.3,-0.26298,-0.90397,-0.33717,134.98 |
| 5796 | | |
| 5797 | | > ui mousemode right select |
| 5798 | | |
| 5799 | | > select clear |
| 5800 | | |
| 5801 | | > select #37/A |
| 5802 | | |
| 5803 | | 2098 atoms, 2129 bonds, 269 residues, 1 model selected |
| 5804 | | |
| 5805 | | > select #41/A |
| 5806 | | |
| 5807 | | 7501 atoms, 7619 bonds, 953 residues, 1 model selected |
| 5808 | | |
| 5809 | | > select #41/D |
| 5810 | | |
| 5811 | | 1420 atoms, 1441 bonds, 177 residues, 1 model selected |
| 5812 | | |
| 5813 | | > delete sel |
| 5814 | | |
| 5815 | | > select #41/C |
| 5816 | | |
| 5817 | | 6830 atoms, 6947 bonds, 874 residues, 1 model selected |
| 5818 | | |
| 5819 | | > delete sel |
| 5820 | | |
| 5821 | | > fitmap #41 inMap #25 |
| 5822 | | |
| 5823 | | Fit molecule fold_20240724_copbdg1z1_try2_model_0.cif (#41) to map |
| 5824 | | W20_J4027_005_volume_map_gammazeta_local.mrc (#25) using 11515 atoms |
| 5825 | | average map value = 0.1733, steps = 152 |
| 5826 | | shifted from previous position = 7.6 |
| 5827 | | rotated from previous position = 10.2 degrees |
| 5828 | | atoms outside contour = 7595, contour level = 0.2532 |
| 5829 | | |
| 5830 | | Position of fold_20240724_copbdg1z1_try2_model_0.cif (#41) relative to |
| 5831 | | W20_J4027_005_volume_map_gammazeta_local.mrc (#25) coordinates: |
| 5832 | | Matrix rotation and translation |
| 5833 | | 0.72372649 0.02528009 -0.68962373 129.00641102 |
| 5834 | | 0.66961476 -0.26733223 0.69292825 143.29008926 |
| 5835 | | -0.16684136 -0.96327275 -0.21040333 139.88692109 |
| 5836 | | Axis -0.89407287 -0.28221546 0.34783348 |
| 5837 | | Axis point 0.00000000 117.92026971 52.51002968 |
| 5838 | | Rotation angle (degrees) 112.14825963 |
| 5839 | | Shift along axis -107.12245762 |
| 5840 | | |
| 5841 | | |
| 5842 | | > show #!25 models |
| 5843 | | |
| 5844 | | > hide #!25 models |
| 5845 | | |
| 5846 | | Drag select of 19 residues |
| 5847 | | |
| 5848 | | > select up |
| 5849 | | |
| 5850 | | 374 atoms, 380 bonds, 50 residues, 1 model selected |
| 5851 | | |
| 5852 | | > select up |
| 5853 | | |
| 5854 | | 4014 atoms, 4083 bonds, 511 residues, 1 model selected |
| 5855 | | |
| 5856 | | > select down |
| 5857 | | |
| 5858 | | 374 atoms, 380 bonds, 50 residues, 1 model selected |
| 5859 | | |
| 5860 | | > delete sel |
| 5861 | | |
| 5862 | | Drag select of 24 residues, 2 pseudobonds |
| 5863 | | |
| 5864 | | > select up |
| 5865 | | |
| 5866 | | 272 atoms, 278 bonds, 2 pseudobonds, 34 residues, 2 models selected |
| 5867 | | |
| 5868 | | > delete sel |
| 5869 | | |
| 5870 | | Drag select of 46 residues, 2 pseudobonds |
| 5871 | | |
| 5872 | | > select up |
| 5873 | | |
| 5874 | | 840 atoms, 846 bonds, 2 pseudobonds, 103 residues, 2 models selected |
| 5875 | | |
| 5876 | | > delete sel |
| 5877 | | |
| 5878 | | Drag select of 81 residues, 2 pseudobonds |
| 5879 | | Drag select of 82 residues, 2 pseudobonds |
| 5880 | | |
| 5881 | | > select clear |
| 5882 | | |
| 5883 | | Drag select of 53 residues |
| 5884 | | |
| 5885 | | > select up |
| 5886 | | |
| 5887 | | 483 atoms, 487 bonds, 61 residues, 1 model selected |
| 5888 | | |
| 5889 | | > select clear |
| 5890 | | |
| 5891 | | Drag select of 82 residues, 2 pseudobonds |
| 5892 | | |
| 5893 | | > select up |
| 5894 | | |
| 5895 | | 844 atoms, 855 bonds, 2 pseudobonds, 108 residues, 2 models selected |
| 5896 | | |
| 5897 | | > delete sel |
| 5898 | | |
| 5899 | | > fitmap #41 inMap #25 |
| 5900 | | |
| 5901 | | Fit molecule fold_20240724_copbdg1z1_try2_model_0.cif (#41) to map |
| 5902 | | W20_J4027_005_volume_map_gammazeta_local.mrc (#25) using 9185 atoms |
| 5903 | | average map value = 0.207, steps = 68 |
| 5904 | | shifted from previous position = 0.487 |
| 5905 | | rotated from previous position = 1.21 degrees |
| 5906 | | atoms outside contour = 5435, contour level = 0.2532 |
| 5907 | | |
| 5908 | | Position of fold_20240724_copbdg1z1_try2_model_0.cif (#41) relative to |
| 5909 | | W20_J4027_005_volume_map_gammazeta_local.mrc (#25) coordinates: |
| 5910 | | Matrix rotation and translation |
| 5911 | | 0.71958143 0.04416992 -0.69300187 128.82756784 |
| 5912 | | 0.67569054 -0.27468426 0.68409856 142.78127561 |
| 5913 | | -0.16014013 -0.96051943 -0.22750289 139.51471009 |
| 5914 | | Axis -0.89355970 -0.28951634 0.34312003 |
| 5915 | | Axis point 0.00000000 115.94606449 53.28137283 |
| 5916 | | Rotation angle (degrees) 113.03559189 |
| 5917 | | Shift along axis -108.58234297 |
| 5918 | | |
| 5919 | | |
| 5920 | | > show #!25 models |
| 5921 | | |
| 5922 | | > hide #!25 models |
| 5923 | | |
| 5924 | | Drag select of 248 residues |
| 5925 | | |
| 5926 | | > select up |
| 5927 | | |
| 5928 | | 2203 atoms, 2233 bonds, 279 residues, 1 model selected |
| 5929 | | |
| 5930 | | > delete sel |
| 5931 | | |
| 5932 | | > fitmap #41 inMap #25 |
| 5933 | | |
| 5934 | | Fit molecule fold_20240724_copbdg1z1_try2_model_0.cif (#41) to map |
| 5935 | | W20_J4027_005_volume_map_gammazeta_local.mrc (#25) using 6982 atoms |
| 5936 | | average map value = 0.2082, steps = 260 |
| 5937 | | shifted from previous position = 7.84 |
| 5938 | | rotated from previous position = 14 degrees |
| 5939 | | atoms outside contour = 4267, contour level = 0.2532 |
| 5940 | | |
| 5941 | | Position of fold_20240724_copbdg1z1_try2_model_0.cif (#41) relative to |
| 5942 | | W20_J4027_005_volume_map_gammazeta_local.mrc (#25) coordinates: |
| 5943 | | Matrix rotation and translation |
| 5944 | | 0.60641983 0.19676174 -0.77041535 127.17385378 |
| 5945 | | 0.79490418 -0.12618975 0.59346734 140.58903166 |
| 5946 | | 0.01955314 -0.97229675 -0.23293070 138.75917394 |
| 5947 | | Axis -0.84500929 -0.42632905 0.32280465 |
| 5948 | | Axis point 0.00000000 109.24582338 58.49339501 |
| 5949 | | Rotation angle (degrees) 112.10779478 |
| 5950 | | Shift along axis -122.60816993 |
| 5951 | | |
| 5952 | | |
| 5953 | | > show #!25 models |
| 5954 | | |
| 5955 | | > hide #!25 models |
| 5956 | | |
| 5957 | | Drag select of 85 residues, 1 pseudobonds |
| 5958 | | |
| 5959 | | > select up |
| 5960 | | |
| 5961 | | 826 atoms, 836 bonds, 1 pseudobond, 108 residues, 2 models selected |
| 5962 | | |
| 5963 | | > delete sel |
| 5964 | | |
| 5965 | | Drag select of 43 residues |
| 5966 | | |
| 5967 | | > select clear |
| 5968 | | |
| 5969 | | Drag select of 67 residues |
| 5970 | | |
| 5971 | | > select up |
| 5972 | | |
| 5973 | | 711 atoms, 719 bonds, 95 residues, 1 model selected |
| 5974 | | |
| 5975 | | > delete sel |
| 5976 | | |
| 5977 | | > fitmap #41 inMap #25 |
| 5978 | | |
| 5979 | | Fit molecule fold_20240724_copbdg1z1_try2_model_0.cif (#41) to map |
| 5980 | | W20_J4027_005_volume_map_gammazeta_local.mrc (#25) using 5445 atoms |
| 5981 | | average map value = 0.1782, steps = 88 |
| 5982 | | shifted from previous position = 2.03 |
| 5983 | | rotated from previous position = 3.11 degrees |
| 5984 | | atoms outside contour = 3752, contour level = 0.2532 |
| 5985 | | |
| 5986 | | Position of fold_20240724_copbdg1z1_try2_model_0.cif (#41) relative to |
| 5987 | | W20_J4027_005_volume_map_gammazeta_local.mrc (#25) coordinates: |
| 5988 | | Matrix rotation and translation |
| 5989 | | 0.60331496 0.23815668 -0.76111264 129.24175666 |
| 5990 | | 0.79728988 -0.15805568 0.58253519 139.72739672 |
| 5991 | | 0.01843647 -0.95827960 -0.28523727 137.10117129 |
| 5992 | | Axis -0.84890646 -0.42948982 0.30805246 |
| 5993 | | Axis point 0.00000000 103.56300999 60.01449132 |
| 5994 | | Rotation angle (degrees) 114.83389261 |
| 5995 | | Shift along axis -127.49130401 |
| 5996 | | |
| 5997 | | |
| 5998 | | > show #!25 models |
| 5999 | | |
| 6000 | | > select add #41 |
| 6001 | | |
| 6002 | | 5445 atoms, 5521 bonds, 8 pseudobonds, 687 residues, 2 models selected |
| 6003 | | Drag select of 25 W20_J4027_005_volume_map_gammazeta_local.mrc , 43 residues |
| 6004 | | |
| 6005 | | > select clear |
| 6006 | | |
| 6007 | | > select add #41 |
| 6008 | | |
| 6009 | | 5445 atoms, 5521 bonds, 8 pseudobonds, 687 residues, 2 models selected |
| 6010 | | |
| 6011 | | > ui mousemode right "translate selected models" |
| 6012 | | |
| 6013 | | > view matrix models |
| 6014 | | > #41,0.60241,0.22967,-0.76444,137.4,0.7981,-0.15867,0.58126,145.75,0.012205,-0.96025,-0.27888,129.95 |
| 6015 | | |
| 6016 | | > fitmap #41 inMap #25 |
| 6017 | | |
| 6018 | | Fit molecule fold_20240724_copbdg1z1_try2_model_0.cif (#41) to map |
| 6019 | | W20_J4027_005_volume_map_gammazeta_local.mrc (#25) using 5445 atoms |
| 6020 | | average map value = 0.203, steps = 116 |
| 6021 | | shifted from previous position = 3.37 |
| 6022 | | rotated from previous position = 14.2 degrees |
| 6023 | | atoms outside contour = 3158, contour level = 0.2532 |
| 6024 | | |
| 6025 | | Position of fold_20240724_copbdg1z1_try2_model_0.cif (#41) relative to |
| 6026 | | W20_J4027_005_volume_map_gammazeta_local.mrc (#25) coordinates: |
| 6027 | | Matrix rotation and translation |
| 6028 | | 0.52191157 0.05705493 -0.85108933 136.78168805 |
| 6029 | | 0.85298460 -0.02899341 0.52113016 146.75941736 |
| 6030 | | 0.00505707 -0.99794996 -0.06379897 132.92819195 |
| 6031 | | Axis -0.79251134 -0.44665565 0.41524030 |
| 6032 | | Axis point 0.00000000 120.55875518 58.21435828 |
| 6033 | | Rotation angle (degrees) 106.58517534 |
| 6034 | | Shift along axis -118.75481890 |
| 6035 | | |
| 6036 | | |
| 6037 | | > select subtract #41 |
| 6038 | | |
| 6039 | | Nothing selected |
| 6040 | | |
| 6041 | | > select add #41 |
| 6042 | | |
| 6043 | | 5445 atoms, 5521 bonds, 8 pseudobonds, 687 residues, 2 models selected |
| 6044 | | |
| 6045 | | > select subtract #41 |
| 6046 | | |
| 6047 | | Nothing selected |
| 6048 | | |
| 6049 | | > select add #41 |
| 6050 | | |
| 6051 | | 5445 atoms, 5521 bonds, 8 pseudobonds, 687 residues, 2 models selected |
| 6052 | | |
| 6053 | | > select subtract #41 |
| 6054 | | |
| 6055 | | Nothing selected |
| 6056 | | |
| 6057 | | > show #35 models |
| 6058 | | |
| 6059 | | > hide #35 models |
| 6060 | | |
| 6061 | | > ui tool show Matchmaker |
| 6062 | | |
| 6063 | | > matchmaker #!37 to #41 |
| 6064 | | |
| 6065 | | Computing secondary structure |
| 6066 | | [Repeated 1 time(s)] Parameters |
| 6067 | | --- |
| 6068 | | Chain pairing | bb |
| 6069 | | Alignment algorithm | Needleman-Wunsch |
| 6070 | | Similarity matrix | BLOSUM-62 |
| 6071 | | SS fraction | 0.3 |
| 6072 | | Gap open (HH/SS/other) | 18/18/6 |
| 6073 | | Gap extend | 1 |
| 6074 | | SS matrix | | | H | S | O |
| 6075 | | ---|---|---|--- |
| 6076 | | H | 6 | -9 | -6 |
| 6077 | | S | | 6 | -6 |
| 6078 | | O | | | 4 |
| 6079 | | Iteration cutoff | 2 |
| 6080 | | |
| 6081 | | Matchmaker fold_20240724_copbdg1z1_try2_model_0.cif, chain B (#41) with |
| 6082 | | CopD_full_Q5XJY5.pdb, chain A (#37), sequence alignment score = 2184.5 |
| 6083 | | RMSD between 114 pruned atom pairs is 0.700 angstroms; (across all 244 pairs: |
| 6084 | | 25.738) |
| 6085 | | |
| 6086 | | |
| 6087 | | > show #!37 models |
| 6088 | | |
| 6089 | | > show #35 models |
| 6090 | | |
| 6091 | | > hide #!41 models |
| 6092 | | |
| 6093 | | > hide #!25 models |
| 6094 | | |
| 6095 | | > ui mousemode right rotate |
| 6096 | | |
| 6097 | | > ui mousemode right select |
| 6098 | | |
| 6099 | | Drag select of 1 residues |
| 6100 | | |
| 6101 | | > select up |
| 6102 | | |
| 6103 | | 184 atoms, 186 bonds, 25 residues, 1 model selected |
| 6104 | | |
| 6105 | | > delete sel |
| 6106 | | |
| 6107 | | > show #!25 models |
| 6108 | | |
| 6109 | | Drag select of 6 residues |
| 6110 | | |
| 6111 | | > select up |
| 6112 | | |
| 6113 | | 483 atoms, 491 bonds, 69 residues, 1 model selected |
| 6114 | | |
| 6115 | | > delete sel |
| 6116 | | |
| 6117 | | > hide #!25 models |
| 6118 | | |
| 6119 | | > show #!25 models |
| 6120 | | |
| 6121 | | > show #!34 models |
| 6122 | | |
| 6123 | | > show #38 models |
| 6124 | | |
| 6125 | | > show #39 models |
| 6126 | | |
| 6127 | | > show #36 models |
| 6128 | | |
| 6129 | | > hide #39 models |
| 6130 | | |
| 6131 | | > hide #!37 models |
| 6132 | | |
| 6133 | | > hide #36 models |
| 6134 | | |
| 6135 | | > hide #35 models |
| 6136 | | |
| 6137 | | > hide #!34 models |
| 6138 | | |
| 6139 | | > hide #!25 models |
| 6140 | | |
| 6141 | | Drag select of 41 residues |
| 6142 | | |
| 6143 | | > select up |
| 6144 | | |
| 6145 | | 531 atoms, 541 bonds, 67 residues, 1 model selected |
| 6146 | | |
| 6147 | | > delete sel |
| 6148 | | |
| 6149 | | Drag select of 26 residues, 1 pseudobonds |
| 6150 | | |
| 6151 | | > select up |
| 6152 | | |
| 6153 | | 494 atoms, 500 bonds, 1 pseudobond, 64 residues, 2 models selected |
| 6154 | | |
| 6155 | | > delete sel |
| 6156 | | |
| 6157 | | Drag select of 3 residues |
| 6158 | | |
| 6159 | | > select up |
| 6160 | | |
| 6161 | | 215 atoms, 218 bonds, 30 residues, 1 model selected |
| 6162 | | |
| 6163 | | > delete sel |
| 6164 | | |
| 6165 | | Drag select of 43 residues, 1 pseudobonds |
| 6166 | | |
| 6167 | | > select up |
| 6168 | | |
| 6169 | | 499 atoms, 507 bonds, 1 pseudobond, 62 residues, 2 models selected |
| 6170 | | |
| 6171 | | > select clear |
| 6172 | | |
| 6173 | | Drag select of 23 residues |
| 6174 | | |
| 6175 | | > select up |
| 6176 | | |
| 6177 | | 296 atoms, 302 bonds, 36 residues, 1 model selected |
| 6178 | | Drag select of 51 residues, 2 pseudobonds |
| 6179 | | |
| 6180 | | > select clear |
| 6181 | | |
| 6182 | | Drag select of 19 residues, 3 pseudobonds |
| 6183 | | |
| 6184 | | > select up |
| 6185 | | |
| 6186 | | 167 atoms, 168 bonds, 3 pseudobonds, 20 residues, 2 models selected |
| 6187 | | |
| 6188 | | > delete sel |
| 6189 | | |
| 6190 | | > show #!25 models |
| 6191 | | |
| 6192 | | > hide #!25 models |
| 6193 | | |
| 6194 | | Drag select of 33 residues |
| 6195 | | |
| 6196 | | > select up |
| 6197 | | |
| 6198 | | 594 atoms, 601 bonds, 75 residues, 1 model selected |
| 6199 | | |
| 6200 | | > delete sel |
| 6201 | | |
| 6202 | | > show #!25 models |
| 6203 | | |
| 6204 | | > hide #!25 models |
| 6205 | | |
| 6206 | | Drag select of 65 residues, 3 pseudobonds |
| 6207 | | |
| 6208 | | > select up |
| 6209 | | |
| 6210 | | 603 atoms, 610 bonds, 3 pseudobonds, 79 residues, 2 models selected |
| 6211 | | |
| 6212 | | > delete sel |
| 6213 | | |
| 6214 | | > show #!25 models |
| 6215 | | |
| 6216 | | > fitmap #38 inMap #25 |
| 6217 | | |
| 6218 | | Fit molecule CopG1_ Q9QZE5.pdb (#38) to map |
| 6219 | | W20_J4027_005_volume_map_gammazeta_local.mrc (#25) using 4226 atoms |
| 6220 | | average map value = 0.2729, steps = 96 |
| 6221 | | shifted from previous position = 0.601 |
| 6222 | | rotated from previous position = 5.3 degrees |
| 6223 | | atoms outside contour = 1993, contour level = 0.2532 |
| 6224 | | |
| 6225 | | Position of CopG1_ Q9QZE5.pdb (#38) relative to |
| 6226 | | W20_J4027_005_volume_map_gammazeta_local.mrc (#25) coordinates: |
| 6227 | | Matrix rotation and translation |
| 6228 | | -0.43244711 0.82798862 0.35696546 106.28810019 |
| 6229 | | -0.18802207 0.30438532 -0.93380795 122.80007772 |
| 6230 | | -0.88183740 -0.47093993 0.02404958 119.35137932 |
| 6231 | | Axis 0.27755203 0.74283001 -0.60923595 |
| 6232 | | Axis point 83.94095393 0.00000000 76.41437643 |
| 6233 | | Rotation angle (degrees) 123.50474947 |
| 6234 | | Shift along axis 48.00691086 |
| 6235 | | |
| 6236 | | |
| 6237 | | > show #39 models |
| 6238 | | |
| 6239 | | > fitmap #39 inMap #25 |
| 6240 | | |
| 6241 | | Fit molecule CopZ1_P61924.pdb (#39) to map |
| 6242 | | W20_J4027_005_volume_map_gammazeta_local.mrc (#25) using 1420 atoms |
| 6243 | | average map value = 0.2375, steps = 180 |
| 6244 | | shifted from previous position = 1.19 |
| 6245 | | rotated from previous position = 45.5 degrees |
| 6246 | | atoms outside contour = 765, contour level = 0.2532 |
| 6247 | | |
| 6248 | | Position of CopZ1_P61924.pdb (#39) relative to |
| 6249 | | W20_J4027_005_volume_map_gammazeta_local.mrc (#25) coordinates: |
| 6250 | | Matrix rotation and translation |
| 6251 | | 0.15949015 0.95372824 0.25488301 116.12737830 |
| 6252 | | 0.29301360 -0.29228658 0.91033598 142.77739950 |
| 6253 | | 0.94271201 -0.07050544 -0.32607217 99.85061617 |
| 6254 | | Axis -0.71693601 -0.50276157 -0.48294261 |
| 6255 | | Axis point 0.00000000 31.13613117 -0.38312474 |
| 6256 | | Rotation angle (degrees) 136.83898997 |
| 6257 | | Shift along axis -203.26100587 |
| 6258 | | |
| 6259 | | |
| 6260 | | > undo |
| 6261 | | |
| 6262 | | > show #39 models |
| 6263 | | |
| 6264 | | > ui tool show Matchmaker |
| 6265 | | |
| 6266 | | > matchmaker #39 to #40 |
| 6267 | | |
| 6268 | | Computing secondary structure |
| 6269 | | [Repeated 1 time(s)] Parameters |
| 6270 | | --- |
| 6271 | | Chain pairing | bb |
| 6272 | | Alignment algorithm | Needleman-Wunsch |
| 6273 | | Similarity matrix | BLOSUM-62 |
| 6274 | | SS fraction | 0.3 |
| 6275 | | Gap open (HH/SS/other) | 18/18/6 |
| 6276 | | Gap extend | 1 |
| 6277 | | SS matrix | | | H | S | O |
| 6278 | | ---|---|---|--- |
| 6279 | | H | 6 | -9 | -6 |
| 6280 | | S | | 6 | -6 |
| 6281 | | O | | | 4 |
| 6282 | | Iteration cutoff | 2 |
| 6283 | | |
| 6284 | | Matchmaker fold_2025_01_17_alpha1_330gamma1_330zeta_model_0.cif, chain C (#40) |
| 6285 | | with CopZ1_P61924.pdb, chain A (#39), sequence alignment score = 863.4 |
| 6286 | | RMSD between 141 pruned atom pairs is 0.407 angstroms; (across all 177 pairs: |
| 6287 | | 16.666) |
| 6288 | | |
| 6289 | | |
| 6290 | | > select clear |
| 6291 | | |
| 6292 | | > show #!34 models |
| 6293 | | |
| 6294 | | > show #35 models |
| 6295 | | |
| 6296 | | > show #36 models |
| 6297 | | |
| 6298 | | > show #!37 models |
| 6299 | | |
| 6300 | | > save "/Users/becca/Desktop/Postdoc/COPI notebooks and |
| 6301 | | > resources/Processing/W20_MM_cyto_COPI/20250701_W20_COPI_cyto_alignments.cxs" |
| 6302 | | |
| 6303 | | > hide #!25 models |
| 6304 | | |
| 6305 | | > show #!26 models |
| 6306 | | |
| 6307 | | > hide #!26 models |
| 6308 | | |
| 6309 | | > show #!27 models |
| 6310 | | |
| 6311 | | > save "/Users/becca/Desktop/Postdoc/COPI notebooks and |
| 6312 | | > resources/Processing/W20_MM_cyto_COPI/20250701_W20_COPI_cyto_alignments.cxs" |
| 6313 | | |
| 6314 | | ——— End of log from Tue Jul 1 15:42:09 2025 ——— |
| 6315 | | |
| 6316 | | > view name session-start |
| 6317 | | |
| 6318 | | opened ChimeraX session |
| 6319 | | |
| 6320 | | > show #!1 models |
| 6321 | | |
| 6322 | | > hide #!1 models |
| 6323 | | |
| 6324 | | > hide #!27 models |
| 6325 | | |
| 6326 | | > show #!29 models |
| 6327 | | |
| 6328 | | > hide #!29 models |
| 6329 | | |
| 6330 | | > show #!29 models |
| 6331 | | |
| 6332 | | > volume #29.1 level 0.01218 |
| 6333 | | |
| 6334 | | > volume #29.2 level 0.009354 |
| 6335 | | |
| 6336 | | > volume #29.3 level 0.003817 |
| 6337 | | |
| 6338 | | > volume #29.4 level 0.01012 |
| 6339 | | |
| 6340 | | > hide #!29 models |
| 6341 | | |
| 6342 | | > show #!29 models |
| 6343 | | |
| 6344 | | > color #29 silver models |
| 6345 | | |
| 6346 | | > color #29 darkgrey models |
| 6347 | | |
| 6348 | | > color #29 #a9a9a9c7 models |
| 6349 | | |
| 6350 | | > color #29 darkgrey models |
| 6351 | | |
| 6352 | | > color #29 #929292ff models |
| 6353 | | |
| 6354 | | > color #29 darkgrey models |
| 6355 | | |
| 6356 | | > show #!30 models |
| 6357 | | |
| 6358 | | > volume #30.1 level 0.004859 |
| 6359 | | |
| 6360 | | > hide #!29 models |
| 6361 | | |
| 6362 | | > color #30 #929292ff models |
| 6363 | | |
| 6364 | | > volume #30.1 level 0.01271 |
| 6365 | | |
| 6366 | | > volume #30.2 level 0.00973 |
| 6367 | | |
| 6368 | | > volume #30.3 level 0.008159 |
| 6369 | | |
| 6370 | | > volume #30.4 level 0.01229 |
| 6371 | | |
| 6372 | | > hide #!30 models |
| 6373 | | |
| 6374 | | > show #!31 models |
| 6375 | | |
| 6376 | | > volume #31.1 level 0.01583 |
| 6377 | | |
| 6378 | | > color #31 #929292ff models |
| 6379 | | |
| 6380 | | > volume #31.2 level 0.0156 |
| 6381 | | |
| 6382 | | > volume #31.3 level 0.008589 |
| 6383 | | |
| 6384 | | > volume #31.4 level 0.01446 |
| 6385 | | |
| 6386 | | > hide #!31 models |
| 6387 | | |
| 6388 | | > color #32 #929292ff models |
| 6389 | | |
| 6390 | | > show #!32 models |
| 6391 | | |
| 6392 | | > volume #32.1 level 0.01368 |
| 6393 | | |
| 6394 | | > volume #32.3 level 0.01158 |
| 6395 | | |
| 6396 | | > volume #32.4 level 0.01325 |
| 6397 | | |
| 6398 | | > volume #32.2 level 0.01059 |
| 6399 | | |
| 6400 | | > hide #!32 models |
| 6401 | | |
| 6402 | | > show #!33 models |
| 6403 | | |
| 6404 | | > volume #33.1 level 0.01263 |
| 6405 | | |
| 6406 | | > color #33 #929292ff models |
| 6407 | | |
| 6408 | | > volume #33.1 level 0.01481 |
| 6409 | | |
| 6410 | | > volume #33.2 level 0.01481 |
| 6411 | | |
| 6412 | | > volume #33.3 level 0.01517 |
| 6413 | | |
| 6414 | | > volume #33.4 level 0.01344 |
| 6415 | | |
| 6416 | | > hide #!34 models |
| 6417 | | |
| 6418 | | > hide #35 models |
| 6419 | | |
| 6420 | | > hide #36 models |
| 6421 | | |
| 6422 | | > hide #!37 models |
| 6423 | | |
| 6424 | | > hide #!38 models |
| 6425 | | |
| 6426 | | > hide #39 models |
| 6427 | | |
| 6428 | | > show #!41 models |
| 6429 | | |
| 6430 | | > hide #!41 models |
| 6431 | | |
| 6432 | | > show #40 models |
| 6433 | | |
| 6434 | | > hide #40 models |
| 6435 | | |
| 6436 | | > show #!29 models |
| 6437 | | |
| 6438 | | > color #29 #8efa00ff models |
| 6439 | | |
| 6440 | | > color #29 #fffc79ff models |
| 6441 | | |
| 6442 | | > volume #29.1 level 0.01322 |
| 6443 | | |
| 6444 | | > volume #29.2 level 0.0117 |
| 6445 | | |
| 6446 | | > volume #29.3 level 0.01271 |
| 6447 | | |
| 6448 | | > volume #29.4 level 0.01296 |
| 6449 | | |
| 6450 | | > hide #!29 models |
| 6451 | | |
| 6452 | | > show #!29 models |
| 6453 | | |
| 6454 | | > hide #!33 models |
| 6455 | | |
| 6456 | | > show #!30 models |
| 6457 | | |
| 6458 | | > hide #!30 models |
| 6459 | | |
| 6460 | | > show #!31 models |
| 6461 | | |
| 6462 | | > hide #!31 models |
| 6463 | | |
| 6464 | | > show #!32 models |
| 6465 | | |
| 6466 | | > hide #!32 models |
| 6467 | | |
| 6468 | | > show #!33 models |
| 6469 | | |
| 6470 | | > show #!32 models |
| 6471 | | |
| 6472 | | > hide #!32 models |
| 6473 | | |
| 6474 | | > show #!32 models |
| 6475 | | |
| 6476 | | > hide #!32 models |
| 6477 | | |
| 6478 | | > show #!32 models |
| 6479 | | |
| 6480 | | > hide #!32 models |
| 6481 | | |
| 6482 | | > show #!32 models |
| 6483 | | |
| 6484 | | > hide #!33 models |
| 6485 | | |
| 6486 | | > show #!33 models |
| 6487 | | |
| 6488 | | > hide #!32 models |
| 6489 | | |
| 6490 | | > hide #!33 models |
| 6491 | | |
| 6492 | | > show #!30 models |
| 6493 | | |
| 6494 | | > hide #!29 models |
| 6495 | | |
| 6496 | | > volume maximum #30.1 #30.2 #30.3 #30.4 |
| 6497 | | |
| 6498 | | Opened volume maximum as #42, grid size 285,281,252, pixel 1.2, shown at step |
| 6499 | | 1, values float32 |
| 6500 | | |
| 6501 | | > rename #42 W20_J140_MB |
| 6502 | | |
| 6503 | | > hide #!42 models |
| 6504 | | |
| 6505 | | > show #!42 models |
| 6506 | | |
| 6507 | | > volume maximum #31.1 #31.2 #31.3 #31.4 |
| 6508 | | |
| 6509 | | Opened volume maximum as #43, grid size 248,255,253, pixel 1.2, shown at step |
| 6510 | | 1, values float32 |
| 6511 | | |
| 6512 | | > rename #43 W20_J145_MB |
| 6513 | | |
| 6514 | | > hide #!43 models |
| 6515 | | |
| 6516 | | > show #!43 models |
| 6517 | | |
| 6518 | | > volume maximum #32.1 #32.2 #32.3 #32.4 |
| 6519 | | |
| 6520 | | Opened volume maximum as #44, grid size 275,269,255, pixel 1.2, shown at step |
| 6521 | | 1, values float32 |
| 6522 | | |
| 6523 | | > rename #44 W20_J150_MB |
| 6524 | | |
| 6525 | | > hide #!44 models |
| 6526 | | |
| 6527 | | > show #!44 models |
| 6528 | | |
| 6529 | | > volume maximum #33.1 #33.2 #33.3 #33.4 |
| 6530 | | |
| 6531 | | Opened volume maximum as #45, grid size 299,295,317, pixel 1.2, shown at step |
| 6532 | | 1, values float32 |
| 6533 | | |
| 6534 | | > rename #45 W20_J155_MB |
| 6535 | | |
| 6536 | | > volume maximum #34.1 #34.2 #34.3 #34.4 |
| 6537 | | |
| 6538 | | [Repeated 3 time(s)] |
| 6539 | | |
| 6540 | | > close #42 |
| 6541 | | |
| 6542 | | > close #43 |
| 6543 | | |
| 6544 | | > close #44 |
| 6545 | | |
| 6546 | | > close #45 |
| 6547 | | |
| 6548 | | > volume maximum #34.1 #34.2 #34.3 #34.4 |
| 6549 | | |
| 6550 | | > volume maximum #29.1 #29.2 #29.3 #29.4 |
| 6551 | | |
| 6552 | | Opened volume maximum as #42, grid size 281,271,305, pixel 1.2, shown at step |
| 6553 | | 1, values float32 |
| 6554 | | |
| 6555 | | > volume maximum #30.1 #29.2 #29.3 #29.4 |
| 6556 | | |
| 6557 | | Opened volume maximum as #43, grid size 324,314,331, pixel 1.2, shown at step |
| 6558 | | 1, values float32 |
| 6559 | | |
| 6560 | | > close #43 |
| 6561 | | |
| 6562 | | > volume maximum #30.1 #30.2 #30.3 #30.4 |
| 6563 | | |
| 6564 | | Opened volume maximum as #43, grid size 285,281,252, pixel 1.2, shown at step |
| 6565 | | 1, values float32 |
| 6566 | | |
| 6567 | | > volume maximum #31.1 #31.2 #31.3 #31.4 |
| 6568 | | |
| 6569 | | Opened volume maximum as #44, grid size 248,255,253, pixel 1.2, shown at step |
| 6570 | | 1, values float32 |
| 6571 | | |
| 6572 | | > volume maximum #32.1 #32.2 #32.3 #32.4 |
| 6573 | | |
| 6574 | | Opened volume maximum as #45, grid size 275,269,255, pixel 1.2, shown at step |
| 6575 | | 1, values float32 |
| 6576 | | |
| 6577 | | > volume maximum #33.1 #33.2 #33.3 #33.4 |
| 6578 | | |
| 6579 | | Opened volume maximum as #46, grid size 299,295,317, pixel 1.2, shown at step |
| 6580 | | 1, values float32 |
| 6581 | | |
| 6582 | | > rename #42 W20_J140_MB |
| 6583 | | |
| 6584 | | > rename #42 W20_J140_MB_joined |
| 6585 | | |
| 6586 | | > rename #43 W20_J145_MB_joined |
| 6587 | | |
| 6588 | | > rename #44 W20_J150_MB_joined |
| 6589 | | |
| 6590 | | > rename #45 W20_J155_MB_joined |
| 6591 | | |
| 6592 | | > rename #46 W20_J160_MB_joined |
| 6593 | | |
| 6594 | | > fitmap #42 inMap #1 |
| 6595 | | |
| 6596 | | Fit map W20_J140_MB_joined in map W20_J3623_consensus_structure_sharp.mrc |
| 6597 | | using 130669 points |
| 6598 | | correlation = 0.3924, correlation about mean = 0.006354, overlap = 259.4 |
| 6599 | | steps = 144, shift = 7.92, angle = 8.21 degrees |
| 6600 | | |
| 6601 | | Position of W20_J140_MB_joined (#42) relative to |
| 6602 | | W20_J3623_consensus_structure_sharp.mrc (#1) coordinates: |
| 6603 | | Matrix rotation and translation |
| 6604 | | 0.91620955 0.25591004 -0.30833442 2.94589670 |
| 6605 | | -0.21484448 0.96327207 0.16108624 14.27677310 |
| 6606 | | 0.33823352 -0.08134481 0.93753993 -22.28584079 |
| 6607 | | Axis -0.29008545 -0.77366308 -0.56329022 |
| 6608 | | Axis point 63.49232840 0.00000000 -7.08518329 |
| 6609 | | Rotation angle (degrees) 24.69964443 |
| 6610 | | Shift along axis 0.65342200 |
| 6611 | | |
| 6612 | | |
| 6613 | | > fitmap #43 inMap #1 |
| 6614 | | |
| 6615 | | Fit map W20_J145_MB_joined in map W20_J3623_consensus_structure_sharp.mrc |
| 6616 | | using 142902 points |
| 6617 | | correlation = 0.4199, correlation about mean = 0.04049, overlap = 303 |
| 6618 | | steps = 164, shift = 1.19, angle = 6.55 degrees |
| 6619 | | |
| 6620 | | Position of W20_J145_MB_joined (#43) relative to |
| 6621 | | W20_J3623_consensus_structure_sharp.mrc (#1) coordinates: |
| 6622 | | Matrix rotation and translation |
| 6623 | | 0.99583148 0.04008635 0.08193132 -23.34504583 |
| 6624 | | -0.04315588 0.99841798 0.03604305 -2.05514686 |
| 6625 | | -0.08035687 -0.03942862 0.99598602 16.93949740 |
| 6626 | | Axis -0.38234856 0.82217147 -0.42171514 |
| 6627 | | Axis point 196.57839852 0.00000000 294.50364776 |
| 6628 | | Rotation angle (degrees) 5.66401964 |
| 6629 | | Shift along axis 0.09261895 |
| 6630 | | |
| 6631 | | |
| 6632 | | > fitmap #44 inMap #1 |
| 6633 | | |
| 6634 | | Fit map W20_J150_MB_joined in map W20_J3623_consensus_structure_sharp.mrc |
| 6635 | | using 100519 points |
| 6636 | | correlation = 0.4322, correlation about mean = -0.01831, overlap = 243.2 |
| 6637 | | steps = 80, shift = 5.66, angle = 2.97 degrees |
| 6638 | | |
| 6639 | | Position of W20_J150_MB_joined (#44) relative to |
| 6640 | | W20_J3623_consensus_structure_sharp.mrc (#1) coordinates: |
| 6641 | | Matrix rotation and translation |
| 6642 | | 0.99916201 0.02809111 0.02976842 -4.49636094 |
| 6643 | | -0.03208054 0.98915209 0.14334916 -11.35419842 |
| 6644 | | -0.02541866 -0.14418402 0.98922437 24.23768179 |
| 6645 | | Axis -0.96196921 0.18463355 -0.20130992 |
| 6646 | | Axis point 0.00000000 157.84496089 87.73789734 |
| 6647 | | Rotation angle (degrees) 8.59507208 |
| 6648 | | Shift along axis -2.65029098 |
| 6649 | | |
| 6650 | | |
| 6651 | | > fitmap #45 inMap #1 |
| 6652 | | |
| 6653 | | Fit map W20_J155_MB_joined in map W20_J3623_consensus_structure_sharp.mrc |
| 6654 | | using 138129 points |
| 6655 | | correlation = 0.4135, correlation about mean = 0.03029, overlap = 293.3 |
| 6656 | | steps = 136, shift = 5.25, angle = 4.6 degrees |
| 6657 | | |
| 6658 | | Position of W20_J155_MB_joined (#45) relative to |
| 6659 | | W20_J3623_consensus_structure_sharp.mrc (#1) coordinates: |
| 6660 | | Matrix rotation and translation |
| 6661 | | 0.97166385 -0.22776257 -0.06319474 54.87908166 |
| 6662 | | 0.22867640 0.97347338 0.00752908 -17.23256599 |
| 6663 | | 0.05980356 -0.02176688 0.99797281 -6.95123433 |
| 6664 | | Axis -0.06185438 -0.25969395 0.96370799 |
| 6665 | | Axis point 107.95346859 225.99227664 0.00000000 |
| 6666 | | Rotation angle (degrees) 13.69857239 |
| 6667 | | Shift along axis -5.61827826 |
| 6668 | | |
| 6669 | | |
| 6670 | | > fitmap #46 inMap #1 |
| 6671 | | |
| 6672 | | Fit map W20_J160_MB_joined in map W20_J3623_consensus_structure_sharp.mrc |
| 6673 | | using 121938 points |
| 6674 | | correlation = 0.4485, correlation about mean = 0.04548, overlap = 303.4 |
| 6675 | | steps = 176, shift = 10.8, angle = 12.9 degrees |
| 6676 | | |
| 6677 | | Position of W20_J160_MB_joined (#46) relative to |
| 6678 | | W20_J3623_consensus_structure_sharp.mrc (#1) coordinates: |
| 6679 | | Matrix rotation and translation |
| 6680 | | 0.98515785 -0.09819617 0.14078890 -1.18436719 |
| 6681 | | 0.06853050 0.97700616 0.20189734 -32.23116044 |
| 6682 | | -0.15737717 -0.18925241 0.96923472 51.60758570 |
| 6683 | | Axis -0.75318811 0.57414108 0.32104469 |
| 6684 | | Axis point 0.00000000 244.46953938 184.51242914 |
| 6685 | | Rotation angle (degrees) 15.05005024 |
| 6686 | | Shift along axis -1.04484054 |
| 6687 | | |
| 6688 | | |
| 6689 | | > show #!1 models |
| 6690 | | |
| 6691 | | > hide #!1 models |
| 6692 | | |
| 6693 | | > show #!2 models |
| 6694 | | |
| 6695 | | > hide #!2 models |
| 6696 | | |
| 6697 | | > hide #!42 models |
| 6698 | | |
| 6699 | | > hide #!43 models |
| 6700 | | |
| 6701 | | > hide #!44 models |
| 6702 | | |
| 6703 | | > hide #!45 models |
| 6704 | | |
| 6705 | | > hide #!46 models |
| 6706 | | |
| 6707 | | > show #!1 models |
| 6708 | | |
| 6709 | | > show #!2 models |
| 6710 | | |
| 6711 | | > hide #!2 models |
| 6712 | | |
| 6713 | | > show #!3 models |
| 6714 | | |
| 6715 | | > hide #!3 models |
| 6716 | | |
| 6717 | | > show #!4 models |
| 6718 | | |
| 6719 | | > hide #!4 models |
| 6720 | | |
| 6721 | | > show #!5 models |
| 6722 | | |
| 6723 | | > hide #!5 models |
| 6724 | | |
| 6725 | | > show #!6 models |
| 6726 | | |
| 6727 | | > hide #!6 models |
| 6728 | | |
| 6729 | | > show #!7 models |
| 6730 | | |
| 6731 | | > hide #!7 models |
| 6732 | | |
| 6733 | | > show #!8 models |
| 6734 | | |
| 6735 | | > hide #!8 models |
| 6736 | | |
| 6737 | | > show #!9 models |
| 6738 | | |
| 6739 | | > hide #!9 models |
| 6740 | | |
| 6741 | | > show #!10 models |
| 6742 | | |
| 6743 | | > hide #!10 models |
| 6744 | | |
| 6745 | | > show #!11 models |
| 6746 | | |
| 6747 | | > hide #!11 models |
| 6748 | | |
| 6749 | | > show #!12 models |
| 6750 | | |
| 6751 | | > hide #!12 models |
| 6752 | | |
| 6753 | | > show #!13 models |
| 6754 | | |
| 6755 | | > hide #!13 models |
| 6756 | | |
| 6757 | | > show #!14 models |
| 6758 | | |
| 6759 | | > hide #!14 models |
| 6760 | | |
| 6761 | | > show #!15 models |
| 6762 | | |
| 6763 | | > hide #!15 models |
| 6764 | | |
| 6765 | | > show #!16 models |
| 6766 | | |
| 6767 | | > hide #!16 models |
| 6768 | | |
| 6769 | | > show #!17 models |
| 6770 | | |
| 6771 | | > hide #!17 models |
| 6772 | | |
| 6773 | | > show #!18 models |
| 6774 | | |
| 6775 | | > hide #!18 models |
| 6776 | | |
| 6777 | | > show #!19 models |
| 6778 | | |
| 6779 | | > hide #!19 models |
| 6780 | | |
| 6781 | | > show #!20 models |
| 6782 | | |
| 6783 | | > hide #!20 models |
| 6784 | | |
| 6785 | | > show #!21 models |
| 6786 | | |
| 6787 | | > hide #!21 models |
| 6788 | | |
| 6789 | | > show #!22 models |
| 6790 | | |
| 6791 | | > hide #!22 models |
| 6792 | | |
| 6793 | | > show #!23 models |
| 6794 | | |
| 6795 | | > hide #!23 models |
| 6796 | | |
| 6797 | | > show #!24 models |
| 6798 | | |
| 6799 | | > hide #!24 models |
| 6800 | | |
| 6801 | | > show #!25 models |
| 6802 | | |
| 6803 | | > hide #!25 models |
| 6804 | | |
| 6805 | | > show #!25 models |
| 6806 | | |
| 6807 | | > color #25 silver models |
| 6808 | | |
| 6809 | | [Repeated 1 time(s)] |
| 6810 | | |
| 6811 | | > hide #!25 models |
| 6812 | | |
| 6813 | | > show #!26 models |
| 6814 | | |
| 6815 | | > hide #!26 models |
| 6816 | | |
| 6817 | | > show #!27 models |
| 6818 | | |
| 6819 | | > hide #!27 models |
| 6820 | | |
| 6821 | | > show #!28 models |
| 6822 | | |
| 6823 | | > hide #!28 models |
| 6824 | | |
| 6825 | | > show #!29 models |
| 6826 | | |
| 6827 | | > hide #!29 models |
| 6828 | | |
| 6829 | | > hide #!30 models |
| 6830 | | |
| 6831 | | > show #!30 models |
| 6832 | | |
| 6833 | | > hide #!30 models |
| 6834 | | |
| 6835 | | > show #!29 models |
| 6836 | | |
| 6837 | | > hide #!29 models |
| 6838 | | |
| 6839 | | > show #!30 models |
| 6840 | | |
| 6841 | | > hide #!30 models |
| 6842 | | |
| 6843 | | > show #!30.1 models |
| 6844 | | |
| 6845 | | > show #!30.2 models |
| 6846 | | |
| 6847 | | > show #!30.3 models |
| 6848 | | |
| 6849 | | > show #!30.4 models |
| 6850 | | |
| 6851 | | > show #!29 models |
| 6852 | | |
| 6853 | | > hide #!29 models |
| 6854 | | |
| 6855 | | > hide #!30 models |
| 6856 | | |
| 6857 | | > show #!30 models |
| 6858 | | |
| 6859 | | > hide #!30 models |
| 6860 | | |
| 6861 | | > show #!30 models |
| 6862 | | |
| 6863 | | > hide #!30 models |
| 6864 | | |
| 6865 | | > show #!30 models |
| 6866 | | |
| 6867 | | > hide #!30 models |
| 6868 | | |
| 6869 | | > show #!29.1 models |
| 6870 | | |
| 6871 | | > show #!29.2 models |
| 6872 | | |
| 6873 | | > show #!29.3 models |
| 6874 | | |
| 6875 | | > show #!29.4 models |
| 6876 | | |
| 6877 | | > hide #!29 models |
| 6878 | | |
| 6879 | | > show #!31 models |
| 6880 | | |
| 6881 | | > show #!31.1 models |
| 6882 | | |
| 6883 | | > show #!31.2 models |
| 6884 | | |
| 6885 | | > show #!31.3 models |
| 6886 | | |
| 6887 | | > show #!31.4 models |
| 6888 | | |
| 6889 | | > hide #!31 models |
| 6890 | | |
| 6891 | | > show #!31 models |
| 6892 | | |
| 6893 | | > hide #!31 models |
| 6894 | | |
| 6895 | | > show #!31 models |
| 6896 | | |
| 6897 | | > hide #!31 models |
| 6898 | | |
| 6899 | | > show #!32 models |
| 6900 | | |
| 6901 | | > show #!32.1 models |
| 6902 | | |
| 6903 | | > show #!32.2 models |
| 6904 | | |
| 6905 | | > show #!32.3 models |
| 6906 | | |
| 6907 | | > show #!32.4 models |
| 6908 | | |
| 6909 | | > hide #!32 models |
| 6910 | | |
| 6911 | | > select add #32 |
| 6912 | | |
| 6913 | | 9 models selected |
| 6914 | | |
| 6915 | | > select subtract #32 |
| 6916 | | |
| 6917 | | Nothing selected |
| 6918 | | |
| 6919 | | > show #!32 models |
| 6920 | | |
| 6921 | | > hide #!32 models |
| 6922 | | |
| 6923 | | > show #!33 models |
| 6924 | | |
| 6925 | | > show #!33.1 models |
| 6926 | | |
| 6927 | | > show #!33.2 models |
| 6928 | | |
| 6929 | | > show #!33.3 models |
| 6930 | | |
| 6931 | | > show #!33.4 models |
| 6932 | | |
| 6933 | | > hide #!33 models |
| 6934 | | |
| 6935 | | > show #!32 models |
| 6936 | | |
| 6937 | | > hide #!32 models |
| 6938 | | |
| 6939 | | > show #!33 models |
| 6940 | | |
| 6941 | | > hide #!33 models |
| 6942 | | |
| 6943 | | > show #!42 models |
| 6944 | | |
| 6945 | | > hide #!42 models |
| 6946 | | |
| 6947 | | > show #!42 models |
| 6948 | | |
| 6949 | | > show #!43 models |
| 6950 | | |
| 6951 | | > hide #!43 models |
| 6952 | | |
| 6953 | | > select add #42 |
| 6954 | | |
| 6955 | | 2 models selected |
| 6956 | | |
| 6957 | | > view matrix models |
| 6958 | | > #42,0.91621,0.25591,-0.30833,6.5795,-0.21484,0.96327,0.16109,11.618,0.33823,-0.081345,0.93754,-14.738 |
| 6959 | | |
| 6960 | | > ui mousemode right "rotate selected models" |
| 6961 | | |
| 6962 | | > view matrix models |
| 6963 | | > #42,-0.20075,-0.93591,0.28944,250.09,-0.75521,0.33604,0.5628,121.07,-0.62399,-0.10561,-0.77426,360.31 |
| 6964 | | |
| 6965 | | > ui mousemode right "translate selected models" |
| 6966 | | |
| 6967 | | > view matrix models |
| 6968 | | > #42,-0.20075,-0.93591,0.28944,258.45,-0.75521,0.33604,0.5628,111.09,-0.62399,-0.10561,-0.77426,373.71 |
| 6969 | | |
| 6970 | | > ui mousemode right "rotate selected models" |
| 6971 | | |
| 6972 | | > view matrix models |
| 6973 | | > #42,-0.33018,-0.9414,-0.068949,327.26,-0.81305,0.24654,0.52742,137.15,-0.47951,0.2302,-0.8468,314.67 |
| 6974 | | |
| 6975 | | > fitmap #42 inMap #1 |
| 6976 | | |
| 6977 | | Fit map W20_J140_MB_joined in map W20_J3623_consensus_structure_sharp.mrc |
| 6978 | | using 130669 points |
| 6979 | | correlation = 0.8803, correlation about mean = 0.3684, overlap = 1189 |
| 6980 | | steps = 172, shift = 19.9, angle = 7.77 degrees |
| 6981 | | |
| 6982 | | Position of W20_J140_MB_joined (#42) relative to |
| 6983 | | W20_J3623_consensus_structure_sharp.mrc (#1) coordinates: |
| 6984 | | Matrix rotation and translation |
| 6985 | | -0.35155267 -0.92578085 -0.13907028 347.82271473 |
| 6986 | | -0.76197836 0.19666262 0.61701928 136.35497869 |
| 6987 | | -0.54387471 0.32288331 -0.77456224 286.91060622 |
| 6988 | | Axis -0.55865111 0.76884323 0.31110933 |
| 6989 | | Axis point 248.45663259 0.00000000 85.56946066 |
| 6990 | | Rotation angle (degrees) 164.73667959 |
| 6991 | | Shift along axis -0.21537821 |
| 6992 | | |
| 6993 | | |
| 6994 | | > select subtract #42 |
| 6995 | | |
| 6996 | | Nothing selected |
| 6997 | | |
| 6998 | | > hide #!42 models |
| 6999 | | |
| 7000 | | > show #!43 models |
| 7001 | | |
| 7002 | | > select add #43 |
| 7003 | | |
| 7004 | | 2 models selected |
| 7005 | | |
| 7006 | | > view matrix models |
| 7007 | | > #43,-0.71667,-0.59905,-0.3571,362.32,-0.66464,0.43155,0.60993,81.644,-0.21127,0.67446,-0.70744,174.56 |
| 7008 | | |
| 7009 | | > view matrix models |
| 7010 | | > #43,-0.22267,-0.96408,0.14484,277.38,-0.843,0.26503,0.46809,150.16,-0.48966,-0.017866,-0.87173,336.37 |
| 7011 | | |
| 7012 | | > ui mousemode right "translate selected models" |
| 7013 | | |
| 7014 | | > view matrix models |
| 7015 | | > #43,-0.22267,-0.96408,0.14484,278.5,-0.843,0.26503,0.46809,152.18,-0.48966,-0.017866,-0.87173,341.6 |
| 7016 | | |
| 7017 | | > fitmap #43 inMap #1 |
| 7018 | | |
| 7019 | | Fit map W20_J145_MB_joined in map W20_J3623_consensus_structure_sharp.mrc |
| 7020 | | using 142902 points |
| 7021 | | correlation = 0.8839, correlation about mean = 0.3912, overlap = 1241 |
| 7022 | | steps = 176, shift = 16.1, angle = 20.5 degrees |
| 7023 | | |
| 7024 | | Position of W20_J145_MB_joined (#43) relative to |
| 7025 | | W20_J3623_consensus_structure_sharp.mrc (#1) coordinates: |
| 7026 | | Matrix rotation and translation |
| 7027 | | -0.33273365 -0.93244330 -0.14084678 345.90650730 |
| 7028 | | -0.76625576 0.18027360 0.61672809 139.19380919 |
| 7029 | | -0.54967302 0.31313085 -0.77447314 289.28503932 |
| 7030 | | Axis -0.56677541 0.76322385 0.31024988 |
| 7031 | | Axis point 250.48919740 0.00000000 85.44391549 |
| 7032 | | Rotation angle (degrees) 164.46490776 |
| 7033 | | Shift along axis -0.06461673 |
| 7034 | | |
| 7035 | | |
| 7036 | | > select subtract #43 |
| 7037 | | |
| 7038 | | Nothing selected |
| 7039 | | |
| 7040 | | > hide #!43 models |
| 7041 | | |
| 7042 | | > show #!44 models |
| 7043 | | |
| 7044 | | > select add #44 |
| 7045 | | |
| 7046 | | 2 models selected |
| 7047 | | |
| 7048 | | > ui mousemode right "rotate selected models" |
| 7049 | | |
| 7050 | | > view matrix models |
| 7051 | | > #44,-0.64334,-0.25066,-0.72339,371.34,-0.44115,0.89362,0.082686,67.718,0.62571,0.37232,-0.68547,101.18 |
| 7052 | | |
| 7053 | | > view matrix models |
| 7054 | | > #44,-0.33974,-0.91968,0.1969,294.7,-0.6214,0.37665,0.68702,81.003,-0.706,0.11106,-0.69945,324.73 |
| 7055 | | |
| 7056 | | > view matrix models |
| 7057 | | > #44,-0.34685,-0.93744,0.029929,322.46,-0.77694,0.30504,0.55074,132.64,-0.52542,0.16777,-0.83414,311.06 |
| 7058 | | |
| 7059 | | > ui mousemode right "translate selected models" |
| 7060 | | |
| 7061 | | > view matrix models |
| 7062 | | > #44,-0.34685,-0.93744,0.029929,328.6,-0.77694,0.30504,0.55074,126.41,-0.52542,0.16777,-0.83414,322.03 |
| 7063 | | |
| 7064 | | > fitmap #44 inMap #1 |
| 7065 | | |
| 7066 | | Fit map W20_J150_MB_joined in map W20_J3623_consensus_structure_sharp.mrc |
| 7067 | | using 100519 points |
| 7068 | | correlation = 0.8806, correlation about mean = 0.2563, overlap = 1060 |
| 7069 | | steps = 104, shift = 9.91, angle = 11.1 degrees |
| 7070 | | |
| 7071 | | Position of W20_J150_MB_joined (#44) relative to |
| 7072 | | W20_J3623_consensus_structure_sharp.mrc (#1) coordinates: |
| 7073 | | Matrix rotation and translation |
| 7074 | | -0.34988200 -0.92650940 -0.13843016 347.22501351 |
| 7075 | | -0.75857847 0.19350448 0.62218544 135.60290867 |
| 7076 | | -0.54967381 0.32270162 -0.77053382 287.77852170 |
| 7077 | | Axis -0.55901416 0.76762419 0.31345858 |
| 7078 | | Axis point 248.55561653 0.00000000 85.33747140 |
| 7079 | | Rotation angle (degrees) 164.46257054 |
| 7080 | | Shift along axis 0.19501983 |
| 7081 | | |
| 7082 | | |
| 7083 | | > select subtract #44 |
| 7084 | | |
| 7085 | | Nothing selected |
| 7086 | | |
| 7087 | | > hide #!44 models |
| 7088 | | |
| 7089 | | > show #!45 models |
| 7090 | | |
| 7091 | | > ui mousemode right "rotate selected models" |
| 7092 | | |
| 7093 | | > select add #45 |
| 7094 | | |
| 7095 | | 2 models selected |
| 7096 | | |
| 7097 | | > view matrix models |
| 7098 | | > #45,-0.93945,-0.28966,-0.18313,341.27,-0.33735,0.87568,0.34552,26.538,0.060286,0.38637,-0.92037,203.81 |
| 7099 | | |
| 7100 | | > view matrix models |
| 7101 | | > #45,-0.39594,-0.91199,0.10724,318.12,-0.72625,0.38247,0.57121,120.46,-0.56195,0.14828,-0.81377,308.66 |
| 7102 | | |
| 7103 | | > fitmap #45 inMap #1 |
| 7104 | | |
| 7105 | | Fit map W20_J155_MB_joined in map W20_J3623_consensus_structure_sharp.mrc |
| 7106 | | using 138129 points |
| 7107 | | correlation = 0.8776, correlation about mean = 0.3767, overlap = 1250 |
| 7108 | | steps = 140, shift = 15.4, angle = 15.9 degrees |
| 7109 | | |
| 7110 | | Position of W20_J155_MB_joined (#45) relative to |
| 7111 | | W20_J3623_consensus_structure_sharp.mrc (#1) coordinates: |
| 7112 | | Matrix rotation and translation |
| 7113 | | -0.35675611 -0.91947462 -0.16520144 351.40689174 |
| 7114 | | -0.78155048 0.19688467 0.59195886 143.25471986 |
| 7115 | | -0.51176551 0.34029820 -0.78885563 282.02499467 |
| 7116 | | Axis -0.55929726 0.77021311 0.30652624 |
| 7117 | | Axis point 248.57307844 0.00000000 86.50174383 |
| 7118 | | Rotation angle (degrees) 166.99833687 |
| 7119 | | Shift along axis 0.24381166 |
| 7120 | | |
| 7121 | | |
| 7122 | | > select subtract #45 |
| 7123 | | |
| 7124 | | Nothing selected |
| 7125 | | |
| 7126 | | > hide #!45 models |
| 7127 | | |
| 7128 | | > show #!46 models |
| 7129 | | |
| 7130 | | > select add #46 |
| 7131 | | |
| 7132 | | 2 models selected |
| 7133 | | |
| 7134 | | > view matrix models |
| 7135 | | > #46,-0.25068,-0.94965,-0.18794,340.4,-0.93649,0.1887,0.29561,209.1,-0.24527,0.25011,-0.93664,265.98 |
| 7136 | | |
| 7137 | | > view matrix models |
| 7138 | | > #46,-0.071284,-0.98257,0.17165,270.25,-0.5959,0.17996,0.78264,95.4,-0.79989,-0.046498,-0.59834,338.43 |
| 7139 | | |
| 7140 | | > ui mousemode right "translate selected models" |
| 7141 | | |
| 7142 | | > view matrix models |
| 7143 | | > #46,-0.071284,-0.98257,0.17165,275.5,-0.5959,0.17996,0.78264,90.643,-0.79989,-0.046498,-0.59834,355.66 |
| 7144 | | |
| 7145 | | > view matrix models |
| 7146 | | > #46,-0.071284,-0.98257,0.17165,285.03,-0.5959,0.17996,0.78264,91.932,-0.79989,-0.046498,-0.59834,362.22 |
| 7147 | | |
| 7148 | | > fitmap #46 inMap #1 |
| 7149 | | |
| 7150 | | Fit map W20_J160_MB_joined in map W20_J3623_consensus_structure_sharp.mrc |
| 7151 | | using 121938 points |
| 7152 | | correlation = 0.8667, correlation about mean = 0.3087, overlap = 1157 |
| 7153 | | steps = 208, shift = 18.2, angle = 31.4 degrees |
| 7154 | | |
| 7155 | | Position of W20_J160_MB_joined (#46) relative to |
| 7156 | | W20_J3623_consensus_structure_sharp.mrc (#1) coordinates: |
| 7157 | | Matrix rotation and translation |
| 7158 | | -0.39178850 -0.90130925 -0.18477932 357.17449457 |
| 7159 | | -0.76159854 0.20502417 0.61476235 135.64467847 |
| 7160 | | -0.51620677 0.38158448 -0.76676193 274.65181077 |
| 7161 | | Axis -0.54399036 0.77320089 0.32593693 |
| 7162 | | Axis point 245.55803639 0.00000000 83.69005707 |
| 7163 | | Rotation angle (degrees) 167.62426420 |
| 7164 | | Shift along axis 0.10027404 |
| 7165 | | |
| 7166 | | |
| 7167 | | > select subtract #46 |
| 7168 | | |
| 7169 | | Nothing selected |
| 7170 | | |
| 7171 | | > hide #!46 models |
| 7172 | | |
| 7173 | | > show #!46 models |
| 7174 | | |
| 7175 | | > hide #!46 models |
| 7176 | | |
| 7177 | | > save "/Users/becca/Desktop/Postdoc/COPI notebooks and |
| 7178 | | > resources/Processing/W20_MM_cyto_COPI/20250701_W20_COPI_cyto_alignments.cxs" |
| 7179 | | |
| 7180 | | ——— End of log from Wed Jul 2 15:03:33 2025 ——— |
| 7181 | | |
| 7182 | | > view name session-start |
| 7183 | | |
| 7184 | | opened ChimeraX session |
| 7185 | | |
| 7186 | | > hide #!1 models |
| 7187 | | |
| 7188 | | > show #!46 models |
| 7189 | | |
| 7190 | | > show #!45 models |
| 7191 | | |
| 7192 | | > show #!25 models |
| 7193 | | |
| 7194 | | > color #25 #ff2600ff models |
| 7195 | | |
| 7196 | | > color #46 #ff9300ff models |
| 7197 | | |
| 7198 | | > color #45 #fffb00ff models |
| 7199 | | |
| 7200 | | > show #!9 models |
| 7201 | | |
| 7202 | | > color #9 #8efa00ff models |
| 7203 | | |
| 7204 | | > show #!27 models |
| 7205 | | |
| 7206 | | > color #27 #00fdffff models |
| 7207 | | |
| 7208 | | > show #!23 models |
| 7209 | | |
| 7210 | | > color #23 #0433ffff models |
| 7211 | | |
| 7212 | | > hide #!27 models |
| 7213 | | |
| 7214 | | > show #!27 models |
| 7215 | | |
| 7216 | | > hide #!27 models |
| 7217 | | |
| 7218 | | > hide #!25 models |
| 7219 | | |
| 7220 | | > hide #!23 models |
| 7221 | | |
| 7222 | | > hide #!9 models |
| 7223 | | |
| 7224 | | > hide #!45 models |
| 7225 | | |
| 7226 | | > hide #!46 models |
| 7227 | | |
| 7228 | | > show #!46 models |
| 7229 | | |
| 7230 | | > show #!23 models |
| 7231 | | |
| 7232 | | > hide #!46 models |
| 7233 | | |
| 7234 | | > hide #!23 models |
| 7235 | | |
| 7236 | | > show #!46 models |
| 7237 | | |
| 7238 | | > hide #!1 models |
| 7239 | | |
| 7240 | | > save "/Users/becca/Desktop/Postdoc/COPI notebooks and |
| 7241 | | > resources/Processing/W20_MM_cyto_COPI/20250701_W20_COPI_cyto_alignments.cxs" |
| 7242 | | |
| 7243 | | ——— End of log from Wed Jul 2 17:43:06 2025 ——— |
| 7244 | | |
| 7245 | | opened ChimeraX session |
| 7246 | | |
| 7247 | | > color list |
| 7248 | | |
| 7249 | | 28 custom colors: alphav1 |
| 7250 | | |
| 7251 | | , alphav2 |
| 7252 | | |
| 7253 | | , alphav3 |
| 7254 | | |
| 7255 | | , arf1v1 |
| 7256 | | |
| 7257 | | , arf1v2 |
| 7258 | | |
| 7259 | | , arf1v3 |
| 7260 | | |
| 7261 | | , betapv1 |
| 7262 | | |
| 7263 | | , betapv2 |
| 7264 | | |
| 7265 | | , betapv3 |
| 7266 | | |
| 7267 | | , betav1 |
| 7268 | | |
| 7269 | | , betav2 |
| 7270 | | |
| 7271 | | , betav3 |
| 7272 | | |
| 7273 | | , deltav1 |
| 7274 | | |
| 7275 | | , deltav2 |
| 7276 | | |
| 7277 | | , deltav3 |
| 7278 | | |
| 7279 | | , epsilonv1 |
| 7280 | | |
| 7281 | | , epsilonv3 |
| 7282 | | |
| 7283 | | , gammav1 |
| 7284 | | |
| 7285 | | , gammav2 |
| 7286 | | |
| 7287 | | , gammav3 |
| 7288 | | |
| 7289 | | , label_purple |
| 7290 | | |
| 7291 | | , label_purple_v3 |
| 7292 | | |
| 7293 | | , label_red |
| 7294 | | |
| 7295 | | , label_red_v3 |
| 7296 | | |
| 7297 | | , lable_purple_v4 |
| 7298 | | |
| 7299 | | , zetav1 |
| 7300 | | |
| 7301 | | , zetav2 |
| 7302 | | |
| 7303 | | , and zetav3 |
| 7304 | | |
| 7305 | | . |
| 7306 | | 248 builtin colors: alice blue |
| 7307 | | |
| 7308 | | , aliceblue |
| 7309 | | |
| 7310 | | , antique white |
| 7311 | | |
| 7312 | | , antiquewhite |
| 7313 | | |
| 7314 | | , aqua |
| 7315 | | |
| 7316 | | , aquamarine |
| 7317 | | |
| 7318 | | , azure |
| 7319 | | |
| 7320 | | , beige |
| 7321 | | |
| 7322 | | , bisque |
| 7323 | | |
| 7324 | | , black |
| 7325 | | |
| 7326 | | , blanched almond |
| 7327 | | |
| 7328 | | , blanchedalmond |
| 7329 | | |
| 7330 | | , blue |
| 7331 | | |
| 7332 | | , blue violet |
| 7333 | | |
| 7334 | | , blueviolet |
| 7335 | | |
| 7336 | | , brown |
| 7337 | | |
| 7338 | | , burly wood |
| 7339 | | |
| 7340 | | , burlywood |
| 7341 | | |
| 7342 | | , cadet blue |
| 7343 | | |
| 7344 | | , cadetblue |
| 7345 | | |
| 7346 | | , chartreuse |
| 7347 | | |
| 7348 | | , chocolate |
| 7349 | | |
| 7350 | | , coral |
| 7351 | | |
| 7352 | | , cornflower blue |
| 7353 | | |
| 7354 | | , cornflowerblue |
| 7355 | | |
| 7356 | | , cornsilk |
| 7357 | | |
| 7358 | | , crimson |
| 7359 | | |
| 7360 | | , cyan |
| 7361 | | |
| 7362 | | , dark blue |
| 7363 | | |
| 7364 | | , dark cyan |
| 7365 | | |
| 7366 | | , dark goldenrod |
| 7367 | | |
| 7368 | | , dark gray |
| 7369 | | |
| 7370 | | , dark green |
| 7371 | | |
| 7372 | | , dark grey |
| 7373 | | |
| 7374 | | , dark khaki |
| 7375 | | |
| 7376 | | , dark magenta |
| 7377 | | |
| 7378 | | , dark olive green |
| 7379 | | |
| 7380 | | , dark orange |
| 7381 | | |
| 7382 | | , dark orchid |
| 7383 | | |
| 7384 | | , dark red |
| 7385 | | |
| 7386 | | , dark salmon |
| 7387 | | |
| 7388 | | , dark sea green |
| 7389 | | |
| 7390 | | , dark seagreen |
| 7391 | | |
| 7392 | | , dark slate blue |
| 7393 | | |
| 7394 | | , dark slate gray |
| 7395 | | |
| 7396 | | , dark slate grey |
| 7397 | | |
| 7398 | | , dark turquoise |
| 7399 | | |
| 7400 | | , dark violet |
| 7401 | | |
| 7402 | | , darkblue |
| 7403 | | |
| 7404 | | , darkcyan |
| 7405 | | |
| 7406 | | , darkgoldenrod |
| 7407 | | |
| 7408 | | , darkgray |
| 7409 | | |
| 7410 | | , darkgreen |
| 7411 | | |
| 7412 | | , darkgrey |
| 7413 | | |
| 7414 | | , darkkhaki |
| 7415 | | |
| 7416 | | , darkmagenta |
| 7417 | | |
| 7418 | | , darkolivegreen |
| 7419 | | |
| 7420 | | , darkorange |
| 7421 | | |
| 7422 | | , darkorchid |
| 7423 | | |
| 7424 | | , darkred |
| 7425 | | |
| 7426 | | , darksalmon |
| 7427 | | |
| 7428 | | , darkseagreen |
| 7429 | | |
| 7430 | | , darkslateblue |
| 7431 | | |
| 7432 | | , darkslategray |
| 7433 | | |
| 7434 | | , darkslategrey |
| 7435 | | |
| 7436 | | , darkturquoise |
| 7437 | | |
| 7438 | | , darkviolet |
| 7439 | | |
| 7440 | | , deep pink |
| 7441 | | |
| 7442 | | , deep sky blue |
| 7443 | | |
| 7444 | | , deep skyblue |
| 7445 | | |
| 7446 | | , deeppink |
| 7447 | | |
| 7448 | | , deepskyblue |
| 7449 | | |
| 7450 | | , dim gray |
| 7451 | | |
| 7452 | | , dim grey |
| 7453 | | |
| 7454 | | , dimgray |
| 7455 | | |
| 7456 | | , dimgrey |
| 7457 | | |
| 7458 | | , dodger blue |
| 7459 | | |
| 7460 | | , dodgerblue |
| 7461 | | |
| 7462 | | , fire brick |
| 7463 | | |
| 7464 | | , firebrick |
| 7465 | | |
| 7466 | | , floral white |
| 7467 | | |
| 7468 | | , floralwhite |
| 7469 | | |
| 7470 | | , forest green |
| 7471 | | |
| 7472 | | , forestgreen |
| 7473 | | |
| 7474 | | , fuchsia |
| 7475 | | |
| 7476 | | , gainsboro |
| 7477 | | |
| 7478 | | , ghost white |
| 7479 | | |
| 7480 | | , ghostwhite |
| 7481 | | |
| 7482 | | , gold |
| 7483 | | |
| 7484 | | , goldenrod |
| 7485 | | |
| 7486 | | , gray |
| 7487 | | |
| 7488 | | , green |
| 7489 | | |
| 7490 | | , green yellow |
| 7491 | | |
| 7492 | | , greenyellow |
| 7493 | | |
| 7494 | | , grey |
| 7495 | | |
| 7496 | | , honeydew |
| 7497 | | |
| 7498 | | , hot pink |
| 7499 | | |
| 7500 | | , hotpink |
| 7501 | | |
| 7502 | | , indian red |
| 7503 | | |
| 7504 | | , indianred |
| 7505 | | |
| 7506 | | , indigo |
| 7507 | | |
| 7508 | | , ivory |
| 7509 | | |
| 7510 | | , khaki |
| 7511 | | |
| 7512 | | , lavender |
| 7513 | | |
| 7514 | | , lavender blush |
| 7515 | | |
| 7516 | | , lavenderblush |
| 7517 | | |
| 7518 | | , lawn green |
| 7519 | | |
| 7520 | | , lawngreen |
| 7521 | | |
| 7522 | | , lemon chiffon |
| 7523 | | |
| 7524 | | , lemonchiffon |
| 7525 | | |
| 7526 | | , light blue |
| 7527 | | |
| 7528 | | , light coral |
| 7529 | | |
| 7530 | | , light cyan |
| 7531 | | |
| 7532 | | , light goldenrod yellow |
| 7533 | | |
| 7534 | | , light gray |
| 7535 | | |
| 7536 | | , light green |
| 7537 | | |
| 7538 | | , light grey |
| 7539 | | |
| 7540 | | , light pink |
| 7541 | | |
| 7542 | | , light salmon |
| 7543 | | |
| 7544 | | , light sea green |
| 7545 | | |
| 7546 | | , light seagreen |
| 7547 | | |
| 7548 | | , light sky blue |
| 7549 | | |
| 7550 | | , light skyblue |
| 7551 | | |
| 7552 | | , light slate gray |
| 7553 | | |
| 7554 | | , light slate grey |
| 7555 | | |
| 7556 | | , light steel blue |
| 7557 | | |
| 7558 | | , light yellow |
| 7559 | | |
| 7560 | | , lightblue |
| 7561 | | |
| 7562 | | , lightcoral |
| 7563 | | |
| 7564 | | , lightcyan |
| 7565 | | |
| 7566 | | , lightgoldenrodyellow |
| 7567 | | |
| 7568 | | , lightgray |
| 7569 | | |
| 7570 | | , lightgreen |
| 7571 | | |
| 7572 | | , lightgrey |
| 7573 | | |
| 7574 | | , lightpink |
| 7575 | | |
| 7576 | | , lightsalmon |
| 7577 | | |
| 7578 | | , lightseagreen |
| 7579 | | |
| 7580 | | , lightskyblue |
| 7581 | | |
| 7582 | | , lightslategray |
| 7583 | | |
| 7584 | | , lightslategrey |
| 7585 | | |
| 7586 | | , lightsteelblue |
| 7587 | | |
| 7588 | | , lightyellow |
| 7589 | | |
| 7590 | | , lime |
| 7591 | | |
| 7592 | | , lime green |
| 7593 | | |
| 7594 | | , limegreen |
| 7595 | | |
| 7596 | | , linen |
| 7597 | | |
| 7598 | | , magenta |
| 7599 | | |
| 7600 | | , maroon |
| 7601 | | |
| 7602 | | , medium aquamarine |
| 7603 | | |
| 7604 | | , medium blue |
| 7605 | | |
| 7606 | | , medium orchid |
| 7607 | | |
| 7608 | | , medium purple |
| 7609 | | |
| 7610 | | , medium sea green |
| 7611 | | |
| 7612 | | , medium seagreen |
| 7613 | | |
| 7614 | | , medium slate blue |
| 7615 | | |
| 7616 | | , medium spring green |
| 7617 | | |
| 7618 | | , medium turquoise |
| 7619 | | |
| 7620 | | , medium violet red |
| 7621 | | |
| 7622 | | , mediumaquamarine |
| 7623 | | |
| 7624 | | , mediumblue |
| 7625 | | |
| 7626 | | , mediumorchid |
| 7627 | | |
| 7628 | | , mediumpurple |
| 7629 | | |
| 7630 | | , mediumseagreen |
| 7631 | | |
| 7632 | | , mediumslateblue |
| 7633 | | |
| 7634 | | , mediumspringgreen |
| 7635 | | |
| 7636 | | , mediumturquoise |
| 7637 | | |
| 7638 | | , mediumvioletred |
| 7639 | | |
| 7640 | | , midnight blue |
| 7641 | | |
| 7642 | | , midnightblue |
| 7643 | | |
| 7644 | | , mint cream |
| 7645 | | |
| 7646 | | , mintcream |
| 7647 | | |
| 7648 | | , misty rose |
| 7649 | | |
| 7650 | | , mistyrose |
| 7651 | | |
| 7652 | | , moccasin |
| 7653 | | |
| 7654 | | , navajo white |
| 7655 | | |
| 7656 | | , navajowhite |
| 7657 | | |
| 7658 | | , navy |
| 7659 | | |
| 7660 | | , old lace |
| 7661 | | |
| 7662 | | , oldlace |
| 7663 | | |
| 7664 | | , olive |
| 7665 | | |
| 7666 | | , olive drab |
| 7667 | | |
| 7668 | | , olivedrab |
| 7669 | | |
| 7670 | | , orange |
| 7671 | | |
| 7672 | | , orange red |
| 7673 | | |
| 7674 | | , orangered |
| 7675 | | |
| 7676 | | , orchid |
| 7677 | | |
| 7678 | | , pale goldenrod |
| 7679 | | |
| 7680 | | , pale green |
| 7681 | | |
| 7682 | | , pale turquoise |
| 7683 | | |
| 7684 | | , pale violet red |
| 7685 | | |
| 7686 | | , palegoldenrod |
| 7687 | | |
| 7688 | | , palegreen |
| 7689 | | |
| 7690 | | , paleturquoise |
| 7691 | | |
| 7692 | | , palevioletred |
| 7693 | | |
| 7694 | | , papaya whip |
| 7695 | | |
| 7696 | | , papayawhip |
| 7697 | | |
| 7698 | | , peach puff |
| 7699 | | |
| 7700 | | , peachpuff |
| 7701 | | |
| 7702 | | , peru |
| 7703 | | |
| 7704 | | , pink |
| 7705 | | |
| 7706 | | , plum |
| 7707 | | |
| 7708 | | , powder blue |
| 7709 | | |
| 7710 | | , powderblue |
| 7711 | | |
| 7712 | | , purple |
| 7713 | | |
| 7714 | | , rebecca purple |
| 7715 | | |
| 7716 | | , rebeccapurple |
| 7717 | | |
| 7718 | | , red |
| 7719 | | |
| 7720 | | , rosy brown |
| 7721 | | |
| 7722 | | , rosybrown |
| 7723 | | |
| 7724 | | , royal blue |
| 7725 | | |
| 7726 | | , royalblue |
| 7727 | | |
| 7728 | | , saddle brown |
| 7729 | | |
| 7730 | | , saddlebrown |
| 7731 | | |
| 7732 | | , salmon |
| 7733 | | |
| 7734 | | , sandy brown |
| 7735 | | |
| 7736 | | , sandybrown |
| 7737 | | |
| 7738 | | , sea green |
| 7739 | | |
| 7740 | | , seagreen |
| 7741 | | |
| 7742 | | , seashell |
| 7743 | | |
| 7744 | | , sienna |
| 7745 | | |
| 7746 | | , silver |
| 7747 | | |
| 7748 | | , sky blue |
| 7749 | | |
| 7750 | | , skyblue |
| 7751 | | |
| 7752 | | , slate blue |
| 7753 | | |
| 7754 | | , slate gray |
| 7755 | | |
| 7756 | | , slate grey |
| 7757 | | |
| 7758 | | , slateblue |
| 7759 | | |
| 7760 | | , slategray |
| 7761 | | |
| 7762 | | , slategrey |
| 7763 | | |
| 7764 | | , snow |
| 7765 | | |
| 7766 | | , spring green |
| 7767 | | |
| 7768 | | , springgreen |
| 7769 | | |
| 7770 | | , steel blue |
| 7771 | | |
| 7772 | | , steelblue |
| 7773 | | |
| 7774 | | , tan |
| 7775 | | |
| 7776 | | , teal |
| 7777 | | |
| 7778 | | , thistle |
| 7779 | | |
| 7780 | | , tomato |
| 7781 | | |
| 7782 | | , transparent |
| 7783 | | |
| 7784 | | , turquoise |
| 7785 | | |
| 7786 | | , violet |
| 7787 | | |
| 7788 | | , wheat |
| 7789 | | |
| 7790 | | , white |
| 7791 | | |
| 7792 | | , white smoke |
| 7793 | | |
| 7794 | | , whitesmoke |
| 7795 | | |
| 7796 | | , yellow |
| 7797 | | |
| 7798 | | , yellow green |
| 7799 | | |
| 7800 | | , and yellowgreen |
| 7801 | | |
| 7802 | | . |
| 7803 | | |
| 7804 | | > color name gamma_1 #5EAF5A |
| 7805 | | |
| 7806 | | Color 'gamma_1' is opaque: rgb(36.9%, 68.6%, 35.3%) hex: #5eaf5a |
| 7807 | | |
| 7808 | | |
| 7809 | | |
| 7810 | | > color name gamma_2 #83CA6B |
| 7811 | | |
| 7812 | | Color 'gamma_2' is opaque: rgb(51.4%, 79.2%, 42%) hex: #83ca6b |
| 7813 | | |
| 7814 | | |
| 7815 | | |
| 7816 | | > color name gamma_3 #ADE57C |
| 7817 | | |
| 7818 | | Color 'gamma_3' is opaque: rgb(67.8%, 89.8%, 48.6%) hex: #ade57c |
| 7819 | | |
| 7820 | | |
| 7821 | | |
| 7822 | | > color name gamma_4 #C9EA8C |
| 7823 | | |
| 7824 | | Color 'gamma_4' is opaque: rgb(78.8%, 91.8%, 54.9%) hex: #c9ea8c |
| 7825 | | |
| 7826 | | |
| 7827 | | |
| 7828 | | > color name gamma_5 #E1EF9C |
| 7829 | | |
| 7830 | | Color 'gamma_5' is opaque: rgb(88.2%, 93.7%, 61.2%) hex: #e1ef9c |
| 7831 | | |
| 7832 | | |
| 7833 | | |
| 7834 | | > color name gamma_6 #F3FIAD |
| 7835 | | |
| 7836 | | Missing or invalid "color" argument: expected hexadecimal digits after # |
| 7837 | | |
| 7838 | | > color name gamma_6 #F3F1AD |
| 7839 | | |
| 7840 | | Color 'gamma_6' is opaque: rgb(95.3%, 94.5%, 67.8%) hex: #f3f1ad |
| 7841 | | |
| 7842 | | |
| 7843 | | |
| 7844 | | > show #!45 models |
| 7845 | | |
| 7846 | | > hide #!45 models |
| 7847 | | |
| 7848 | | > hide #!46 models |
| 7849 | | |
| 7850 | | > show #!46 models |
| 7851 | | |
| 7852 | | > show #!45 models |
| 7853 | | |
| 7854 | | > show #!25 models |
| 7855 | | |
| 7856 | | > hide #!46 models |
| 7857 | | |
| 7858 | | > hide #!45 models |
| 7859 | | |
| 7860 | | > show #!38 models |
| 7861 | | |
| 7862 | | > show #35 models |
| 7863 | | |
| 7864 | | > color #25 silver models |
| 7865 | | |
| 7866 | | > color #25 #c0c0c074 models |
| 7867 | | |
| 7868 | | > open "/Users/becca/Desktop/Postdoc/COPI notebooks and |
| 7869 | | > resources/Processing/W20_MM_cyto_COPI/Icknield_workshop_files/W20_models/W20_C1_initial_model_v3.pdb" |
| 7870 | | |
| 7871 | | Chain information for W20_C1_initial_model_v3.pdb #47 |
| 7872 | | --- |
| 7873 | | Chain | Description |
| 7874 | | D E | No description available |
| 7875 | | F | No description available |
| 7876 | | G | No description available |
| 7877 | | H | No description available |
| 7878 | | I | No description available |
| 7879 | | J | No description available |
| 7880 | | K | No description available |
| 7881 | | L | No description available |
| 7882 | | |
| 7883 | | |
| 7884 | | > select add #47 |
| 7885 | | |
| 7886 | | 28705 atoms, 29239 bonds, 3 pseudobonds, 3615 residues, 2 models selected |
| 7887 | | |
| 7888 | | > hide sel atoms |
| 7889 | | |
| 7890 | | > show sel cartoons |
| 7891 | | |
| 7892 | | > hide #!38 models |
| 7893 | | |
| 7894 | | > hide #35 models |
| 7895 | | |
| 7896 | | > view matrix models #47,1,0,0,-79.232,0,1,0,36.623,0,0,1,9.1376 |
| 7897 | | |
| 7898 | | > view matrix models #47,1,0,0,-13.804,0,1,0,-20.445,0,0,1,10.191 |
| 7899 | | |
| 7900 | | > view matrix models #47,1,0,0,0.72332,0,1,0,-4.6589,0,0,1,-2.6371 |
| 7901 | | |
| 7902 | | > fitmap #47 inMap #1 |
| 7903 | | |
| 7904 | | Fit molecule W20_C1_initial_model_v3.pdb (#47) to map |
| 7905 | | W20_J3623_consensus_structure_sharp.mrc (#1) using 28705 atoms |
| 7906 | | average map value = 0.301, steps = 80 |
| 7907 | | shifted from previous position = 5.02 |
| 7908 | | rotated from previous position = 0.504 degrees |
| 7909 | | atoms outside contour = 16132, contour level = 0.26383 |
| 7910 | | |
| 7911 | | Position of W20_C1_initial_model_v3.pdb (#47) relative to |
| 7912 | | W20_J3623_consensus_structure_sharp.mrc (#1) coordinates: |
| 7913 | | Matrix rotation and translation |
| 7914 | | 0.99996809 -0.00526689 0.00600723 0.01730039 |
| 7915 | | 0.00528904 0.99997925 -0.00367710 -0.57665128 |
| 7916 | | -0.00598774 0.00370875 0.99997520 0.14412341 |
| 7917 | | Axis 0.41958417 0.68142506 0.59967410 |
| 7918 | | Axis point 54.13790036 0.00000000 -23.49447330 |
| 7919 | | Rotation angle (degrees) 0.50428904 |
| 7920 | | Shift along axis -0.29925859 |
| 7921 | | |
| 7922 | | |
| 7923 | | > select subtract #47 |
| 7924 | | |
| 7925 | | Nothing selected |
| 7926 | | |
| 7927 | | > hide #!47 models |
| 7928 | | |
| 7929 | | > show #!47 models |
| 7930 | | |
| 7931 | | > split #47 chains |
| 7932 | | |
| 7933 | | Split W20_C1_initial_model_v3.pdb (#47) into 9 models |
| 7934 | | Chain information for W20_C1_initial_model_v3.pdb D #47.1 |
| 7935 | | --- |
| 7936 | | Chain | Description |
| 7937 | | D | No description available |
| 7938 | | |
| 7939 | | Chain information for W20_C1_initial_model_v3.pdb E #47.2 |
| 7940 | | --- |
| 7941 | | Chain | Description |
| 7942 | | E | No description available |
| 7943 | | |
| 7944 | | Chain information for W20_C1_initial_model_v3.pdb F #47.3 |
| 7945 | | --- |
| 7946 | | Chain | Description |
| 7947 | | F | No description available |
| 7948 | | |
| 7949 | | Chain information for W20_C1_initial_model_v3.pdb G #47.4 |
| 7950 | | --- |
| 7951 | | Chain | Description |
| 7952 | | G | No description available |
| 7953 | | |
| 7954 | | Chain information for W20_C1_initial_model_v3.pdb H #47.5 |
| 7955 | | --- |
| 7956 | | Chain | Description |
| 7957 | | H | No description available |
| 7958 | | |
| 7959 | | Chain information for W20_C1_initial_model_v3.pdb I #47.6 |
| 7960 | | --- |
| 7961 | | Chain | Description |
| 7962 | | I | No description available |
| 7963 | | |
| 7964 | | Chain information for W20_C1_initial_model_v3.pdb J #47.7 |
| 7965 | | --- |
| 7966 | | Chain | Description |
| 7967 | | J | No description available |
| 7968 | | |
| 7969 | | Chain information for W20_C1_initial_model_v3.pdb K #47.8 |
| 7970 | | --- |
| 7971 | | Chain | Description |
| 7972 | | K | No description available |
| 7973 | | |
| 7974 | | Chain information for W20_C1_initial_model_v3.pdb L #47.9 |
| 7975 | | --- |
| 7976 | | Chain | Description |
| 7977 | | L | No description available |
| 7978 | | |
| 7979 | | |
| 7980 | | > save "/Users/becca/Desktop/Postdoc/COPI notebooks and |
| 7981 | | > resources/Processing/W20_MM_cyto_COPI/20250703_W20_COPI_cyto_alignments.cxs" |
| 7982 | | |
| 7983 | | ——— End of log from Thu Jul 3 17:41:19 2025 ——— |
| 7984 | | |
| 7985 | | opened ChimeraX session |
| 7986 | | |
| 7987 | | > open "/Users/becca/Desktop/Postdoc/COPI notebooks and |
| 7988 | | > resources/Processing/W20_MM_cyto_COPI/Icknield_workshop_files/W20_models/FlexFit_W20_C1_initial_model_v3/W20_C1_initial_model_v3_no_wat_lig.pdb" |
| 7989 | | |
| 7990 | | Chain information for W20_C1_initial_model_v3_no_wat_lig.pdb #48 |
| 7991 | | --- |
| 7992 | | Chain | Description |
| 7993 | | D E | No description available |
| 7994 | | F | No description available |
| 7995 | | G | No description available |
| 7996 | | H | No description available |
| 7997 | | I | No description available |
| 7998 | | J | No description available |
| 7999 | | K | No description available |
| 8000 | | L | No description available |
| 8001 | | |
| 8002 | | |
| 8003 | | > hide #47.1-5,9#!48#!47.6-8 atoms |
| 8004 | | |
| 8005 | | > show #47.1-5,9#!48#!47.6-8 cartoons |
| 8006 | | |
| 8007 | | > hide #!47 models |
| 8008 | | |
| 8009 | | > hide #!48 models |
| 8010 | | |
| 8011 | | > open "/Users/becca/Desktop/Postdoc/COPI notebooks and |
| 8012 | | > resources/Processing/W20_MM_cyto_COPI/Icknield_workshop_files/W20_models/RIBFIND_W20_C1_initial_model_v3/W20_C1_initial_model_v3_no_wat_lig.pdb" |
| 8013 | | |
| 8014 | | Chain information for W20_C1_initial_model_v3_no_wat_lig.pdb #49 |
| 8015 | | --- |
| 8016 | | Chain | Description |
| 8017 | | D E | No description available |
| 8018 | | F | No description available |
| 8019 | | G | No description available |
| 8020 | | H | No description available |
| 8021 | | I | No description available |
| 8022 | | J | No description available |
| 8023 | | K | No description available |
| 8024 | | L | No description available |
| 8025 | | |
| 8026 | | |
| 8027 | | > hide #!49 atoms |
| 8028 | | |
| 8029 | | > show #!49 cartoons |
| 8030 | | |
| 8031 | | > show #!48 models |
| 8032 | | |
| 8033 | | > hide #!48 models |
| 8034 | | |
| 8035 | | > show #!47 models |
| 8036 | | |
| 8037 | | > hide #!47 models |
| 8038 | | |
| 8039 | | > hide #!49 models |
| 8040 | | |
| 8041 | | > open "/Users/becca/Desktop/Postdoc/COPI notebooks and |
| 8042 | | > resources/Processing/W20_MM_cyto_COPI/Icknield_workshop_files/W20_models/FlexFit_W20_C1_initial_model_TEMPyReff_flexfit/W20_C1_initial_model_no_wat_lig.pdb" |
| 8043 | | |
| 8044 | | Chain information for W20_C1_initial_model_no_wat_lig.pdb #50 |
| 8045 | | --- |
| 8046 | | Chain | Description |
| 8047 | | A0 | No description available |
| 8048 | | A1 | No description available |
| 8049 | | A2 | No description available |
| 8050 | | A3 | No description available |
| 8051 | | A4 | No description available |
| 8052 | | A5 | No description available |
| 8053 | | A6 | No description available |
| 8054 | | A7 | No description available |
| 8055 | | A8 | No description available |
| 8056 | | A9 | No description available |
| 8057 | | BA | No description available |
| 8058 | | |
| 8059 | | |
| 8060 | | > hide #!50 atoms |
| 8061 | | |
| 8062 | | > show #!50 cartoons |
| 8063 | | |
| 8064 | | > select add #50 |
| 8065 | | |
| 8066 | | 25890 atoms, 26364 bonds, 22 pseudobonds, 3243 residues, 2 models selected |
| 8067 | | |
| 8068 | | > select subtract #50 |
| 8069 | | |
| 8070 | | Nothing selected |
| 8071 | | |
| 8072 | | > ui tool show Matchmaker |
| 8073 | | |
| 8074 | | > matchmaker #!50 to #34 |
| 8075 | | |
| 8076 | | Computing secondary structure |
| 8077 | | Parameters |
| 8078 | | --- |
| 8079 | | Chain pairing | bb |
| 8080 | | Alignment algorithm | Needleman-Wunsch |
| 8081 | | Similarity matrix | BLOSUM-62 |
| 8082 | | SS fraction | 0.3 |
| 8083 | | Gap open (HH/SS/other) | 18/18/6 |
| 8084 | | Gap extend | 1 |
| 8085 | | SS matrix | | | H | S | O |
| 8086 | | ---|---|---|--- |
| 8087 | | H | 6 | -9 | -6 |
| 8088 | | S | | 6 | -6 |
| 8089 | | O | | | 4 |
| 8090 | | Iteration cutoff | 2 |
| 8091 | | |
| 8092 | | Matchmaker CopA-F8WHL2.pdb, chain A (#34) with |
| 8093 | | W20_C1_initial_model_no_wat_lig.pdb, chain A2 (#50), sequence alignment score |
| 8094 | | = 2196.3 |
| 8095 | | RMSD between 303 pruned atom pairs is 0.597 angstroms; (across all 445 pairs: |
| 8096 | | 4.918) |
| 8097 | | |
| 8098 | | |
| 8099 | | > hide #!25 models |
| 8100 | | |
| 8101 | | > show #!7 models |
| 8102 | | |
| 8103 | | > hide #!7 models |
| 8104 | | |
| 8105 | | > show #!25 models |
| 8106 | | |
| 8107 | | > show #!7 models |
| 8108 | | |
| 8109 | | > hide #!7 models |
| 8110 | | |
| 8111 | | > show #!7 models |
| 8112 | | |
| 8113 | | > hide #!7 models |
| 8114 | | |
| 8115 | | > show #!7 models |
| 8116 | | |
| 8117 | | > hide #!7 models |
| 8118 | | |
| 8119 | | > show #!8 models |
| 8120 | | |
| 8121 | | > hide #!8 models |
| 8122 | | |
| 8123 | | > show #!7 models |
| 8124 | | |
| 8125 | | > hide #!7 models |
| 8126 | | |
| 8127 | | > open |
| 8128 | | > /Users/becca/Desktop/Postdoc/Phenix/W20_MM_cyto_COPI/PredictAndBuildCryoEM_1/PredictAndBuild_1/local_dock_and_rebuild_6_1/run_mr/A_fixed_model_2_A.pdb |
| 8129 | | |
| 8130 | | Summary of feedback from opening |
| 8131 | | /Users/becca/Desktop/Postdoc/Phenix/W20_MM_cyto_COPI/PredictAndBuildCryoEM_1/PredictAndBuild_1/local_dock_and_rebuild_6_1/run_mr/A_fixed_model_2_A.pdb |
| 8132 | | --- |
| 8133 | | warning | Ignored bad PDB record found on line 9748 |
| 8134 | | |
| 8135 | | Chain information for A_fixed_model_2_A.pdb #51 |
| 8136 | | --- |
| 8137 | | Chain | Description |
| 8138 | | A | No description available |
| 8139 | | |
| 8140 | | Computing secondary structure |
| 8141 | | |
| 8142 | | > open |
| 8143 | | > /Users/becca/Desktop/Postdoc/Phenix/W20_MM_cyto_COPI/PredictAndBuildCryoEM_1/PredictAndBuild_1/local_dock_and_rebuild_6_1/run_mr/B_fixed_model_2_B.pdb |
| 8144 | | |
| 8145 | | Summary of feedback from opening |
| 8146 | | /Users/becca/Desktop/Postdoc/Phenix/W20_MM_cyto_COPI/PredictAndBuildCryoEM_1/PredictAndBuild_1/local_dock_and_rebuild_6_1/run_mr/B_fixed_model_2_B.pdb |
| 8147 | | --- |
| 8148 | | warning | Ignored bad PDB record found on line 7507 |
| 8149 | | |
| 8150 | | Chain information for B_fixed_model_2_B.pdb #52 |
| 8151 | | --- |
| 8152 | | Chain | Description |
| 8153 | | B | No description available |
| 8154 | | |
| 8155 | | Computing secondary structure |
| 8156 | | |
| 8157 | | > open |
| 8158 | | > /Users/becca/Desktop/Postdoc/Phenix/W20_MM_cyto_COPI/PredictAndBuildCryoEM_1/PredictAndBuild_1/local_dock_and_rebuild_6_1/run_mr/C1_fixed_model_2_C1.pdb |
| 8159 | | |
| 8160 | | Summary of feedback from opening |
| 8161 | | /Users/becca/Desktop/Postdoc/Phenix/W20_MM_cyto_COPI/PredictAndBuildCryoEM_1/PredictAndBuild_1/local_dock_and_rebuild_6_1/run_mr/C1_fixed_model_2_C1.pdb |
| 8162 | | --- |
| 8163 | | warning | Ignored bad PDB record found on line 3967 |
| 8164 | | |
| 8165 | | Chain information for C1_fixed_model_2_C1.pdb #53 |
| 8166 | | --- |
| 8167 | | Chain | Description |
| 8168 | | C1 | No description available |
| 8169 | | |
| 8170 | | Computing secondary structure |
| 8171 | | |
| 8172 | | > open |
| 8173 | | > /Users/becca/Desktop/Postdoc/Phenix/W20_MM_cyto_COPI/PredictAndBuildCryoEM_1/PredictAndBuild_1/local_dock_and_rebuild_6_1/run_mr/D1_fixed_model_2_D1.pdb |
| 8174 | | |
| 8175 | | Summary of feedback from opening |
| 8176 | | /Users/becca/Desktop/Postdoc/Phenix/W20_MM_cyto_COPI/PredictAndBuildCryoEM_1/PredictAndBuild_1/local_dock_and_rebuild_6_1/run_mr/D1_fixed_model_2_D1.pdb |
| 8177 | | --- |
| 8178 | | warning | Ignored bad PDB record found on line 3307 |
| 8179 | | |
| 8180 | | Chain information for D1_fixed_model_2_D1.pdb #54 |
| 8181 | | --- |
| 8182 | | Chain | Description |
| 8183 | | D1 | No description available |
| 8184 | | |
| 8185 | | Computing secondary structure |
| 8186 | | |
| 8187 | | > open |
| 8188 | | > /Users/becca/Desktop/Postdoc/Phenix/W20_MM_cyto_COPI/PredictAndBuildCryoEM_1/PredictAndBuild_1/local_dock_and_rebuild_6_1/run_mr/E2_full_shifted_sm_1.pdb |
| 8189 | | |
| 8190 | | Summary of feedback from opening |
| 8191 | | /Users/becca/Desktop/Postdoc/Phenix/W20_MM_cyto_COPI/PredictAndBuildCryoEM_1/PredictAndBuild_1/local_dock_and_rebuild_6_1/run_mr/E2_full_shifted_sm_1.pdb |
| 8192 | | --- |
| 8193 | | warning | Ignored bad PDB record found on line 4486 |
| 8194 | | |
| 8195 | | Chain information for E2_full_shifted_sm_1.pdb #55 |
| 8196 | | --- |
| 8197 | | Chain | Description |
| 8198 | | E2 | No description available |
| 8199 | | |
| 8200 | | Computing secondary structure |
| 8201 | | |
| 8202 | | > open |
| 8203 | | > /Users/becca/Desktop/Postdoc/Phenix/W20_MM_cyto_COPI/PredictAndBuildCryoEM_1/PredictAndBuild_1/local_dock_and_rebuild_6_1/run_mr/F_fixed_model_2_F.pdb |
| 8204 | | |
| 8205 | | Summary of feedback from opening |
| 8206 | | /Users/becca/Desktop/Postdoc/Phenix/W20_MM_cyto_COPI/PredictAndBuildCryoEM_1/PredictAndBuild_1/local_dock_and_rebuild_6_1/run_mr/F_fixed_model_2_F.pdb |
| 8207 | | --- |
| 8208 | | warning | Ignored bad PDB record found on line 1426 |
| 8209 | | |
| 8210 | | Chain information for F_fixed_model_2_F.pdb #56 |
| 8211 | | --- |
| 8212 | | Chain | Description |
| 8213 | | F | No description available |
| 8214 | | |
| 8215 | | Computing secondary structure |
| 8216 | | |
| 8217 | | > hide #!25 models |
| 8218 | | |
| 8219 | | > show #!1 models |
| 8220 | | |
| 8221 | | > hide #56 models |
| 8222 | | |
| 8223 | | > hide #!55 models |
| 8224 | | |
| 8225 | | > hide #!54 models |
| 8226 | | |
| 8227 | | > hide #!53 models |
| 8228 | | |
| 8229 | | > hide #52 models |
| 8230 | | |
| 8231 | | > hide #51 models |
| 8232 | | |
| 8233 | | > hide #!1 models |
| 8234 | | |
| 8235 | | > show #!2 models |
| 8236 | | |
| 8237 | | > color #2 darkgrey models |
| 8238 | | |
| 8239 | | > color #2 #a9a9a980 models |
| 8240 | | |
| 8241 | | > hide #!50 models |
| 8242 | | |
| 8243 | | > show #!47 models |
| 8244 | | |
| 8245 | | > fitmap #47 inMap #2 |
| 8246 | | |
| 8247 | | Fit molecules W20_C1_initial_model_v3.pdb D (#47.1), |
| 8248 | | W20_C1_initial_model_v3.pdb E (#47.2), W20_C1_initial_model_v3.pdb F (#47.3), |
| 8249 | | W20_C1_initial_model_v3.pdb G (#47.4), W20_C1_initial_model_v3.pdb H (#47.5), |
| 8250 | | W20_C1_initial_model_v3.pdb I (#47.6), W20_C1_initial_model_v3.pdb J (#47.7), |
| 8251 | | W20_C1_initial_model_v3.pdb K (#47.8), W20_C1_initial_model_v3.pdb L (#47.9) |
| 8252 | | to map W20_J3623_consensus_structure.mrc (#2) using 28705 atoms |
| 8253 | | average map value = 0.26, steps = 44 |
| 8254 | | shifted from previous position = 0.0505 |
| 8255 | | rotated from previous position = 0.167 degrees |
| 8256 | | atoms outside contour = 16402, contour level = 0.25746 |
| 8257 | | |
| 8258 | | Position of W20_C1_initial_model_v3.pdb D (#47.1) relative to |
| 8259 | | W20_J3623_consensus_structure.mrc (#2) coordinates: |
| 8260 | | Matrix rotation and translation |
| 8261 | | 0.99995987 -0.00698720 0.00560699 0.30640707 |
| 8262 | | 0.00699483 0.99997464 -0.00134153 -1.12673692 |
| 8263 | | -0.00559747 0.00138070 0.99998338 0.47678248 |
| 8264 | | Axis 0.15020785 0.61824110 0.77150214 |
| 8265 | | Axis point 136.27032262 49.14800743 0.00000000 |
| 8266 | | Rotation angle (degrees) 0.51919628 |
| 8267 | | Shift along axis -0.28273161 |
| 8268 | | |
| 8269 | | Position of W20_C1_initial_model_v3.pdb E (#47.2) relative to |
| 8270 | | W20_J3623_consensus_structure.mrc (#2) coordinates: |
| 8271 | | Matrix rotation and translation |
| 8272 | | 0.99995987 -0.00698720 0.00560699 0.30640707 |
| 8273 | | 0.00699483 0.99997464 -0.00134153 -1.12673692 |
| 8274 | | -0.00559747 0.00138070 0.99998338 0.47678248 |
| 8275 | | Axis 0.15020785 0.61824110 0.77150214 |
| 8276 | | Axis point 136.27032262 49.14800743 0.00000000 |
| 8277 | | Rotation angle (degrees) 0.51919628 |
| 8278 | | Shift along axis -0.28273161 |
| 8279 | | |
| 8280 | | Position of W20_C1_initial_model_v3.pdb F (#47.3) relative to |
| 8281 | | W20_J3623_consensus_structure.mrc (#2) coordinates: |
| 8282 | | Matrix rotation and translation |
| 8283 | | 0.99995987 -0.00698720 0.00560699 0.30640707 |
| 8284 | | 0.00699483 0.99997464 -0.00134153 -1.12673692 |
| 8285 | | -0.00559747 0.00138070 0.99998338 0.47678248 |
| 8286 | | Axis 0.15020785 0.61824110 0.77150214 |
| 8287 | | Axis point 136.27032262 49.14800743 0.00000000 |
| 8288 | | Rotation angle (degrees) 0.51919628 |
| 8289 | | Shift along axis -0.28273161 |
| 8290 | | |
| 8291 | | Position of W20_C1_initial_model_v3.pdb G (#47.4) relative to |
| 8292 | | W20_J3623_consensus_structure.mrc (#2) coordinates: |
| 8293 | | Matrix rotation and translation |
| 8294 | | 0.99995987 -0.00698720 0.00560699 0.30640707 |
| 8295 | | 0.00699483 0.99997464 -0.00134153 -1.12673692 |
| 8296 | | -0.00559747 0.00138070 0.99998338 0.47678248 |
| 8297 | | Axis 0.15020785 0.61824110 0.77150214 |
| 8298 | | Axis point 136.27032262 49.14800743 0.00000000 |
| 8299 | | Rotation angle (degrees) 0.51919628 |
| 8300 | | Shift along axis -0.28273161 |
| 8301 | | |
| 8302 | | Position of W20_C1_initial_model_v3.pdb H (#47.5) relative to |
| 8303 | | W20_J3623_consensus_structure.mrc (#2) coordinates: |
| 8304 | | Matrix rotation and translation |
| 8305 | | 0.99995987 -0.00698720 0.00560699 0.30640707 |
| 8306 | | 0.00699483 0.99997464 -0.00134153 -1.12673692 |
| 8307 | | -0.00559747 0.00138070 0.99998338 0.47678248 |
| 8308 | | Axis 0.15020785 0.61824110 0.77150214 |
| 8309 | | Axis point 136.27032262 49.14800743 0.00000000 |
| 8310 | | Rotation angle (degrees) 0.51919628 |
| 8311 | | Shift along axis -0.28273161 |
| 8312 | | |
| 8313 | | Position of W20_C1_initial_model_v3.pdb I (#47.6) relative to |
| 8314 | | W20_J3623_consensus_structure.mrc (#2) coordinates: |
| 8315 | | Matrix rotation and translation |
| 8316 | | 0.99995987 -0.00698720 0.00560699 0.30640707 |
| 8317 | | 0.00699483 0.99997464 -0.00134153 -1.12673692 |
| 8318 | | -0.00559747 0.00138070 0.99998338 0.47678248 |
| 8319 | | Axis 0.15020785 0.61824110 0.77150214 |
| 8320 | | Axis point 136.27032262 49.14800743 0.00000000 |
| 8321 | | Rotation angle (degrees) 0.51919628 |
| 8322 | | Shift along axis -0.28273161 |
| 8323 | | |
| 8324 | | Position of W20_C1_initial_model_v3.pdb J (#47.7) relative to |
| 8325 | | W20_J3623_consensus_structure.mrc (#2) coordinates: |
| 8326 | | Matrix rotation and translation |
| 8327 | | 0.99995987 -0.00698720 0.00560699 0.30640707 |
| 8328 | | 0.00699483 0.99997464 -0.00134153 -1.12673692 |
| 8329 | | -0.00559747 0.00138070 0.99998338 0.47678248 |
| 8330 | | Axis 0.15020785 0.61824110 0.77150214 |
| 8331 | | Axis point 136.27032262 49.14800743 0.00000000 |
| 8332 | | Rotation angle (degrees) 0.51919628 |
| 8333 | | Shift along axis -0.28273161 |
| 8334 | | |
| 8335 | | Position of W20_C1_initial_model_v3.pdb K (#47.8) relative to |
| 8336 | | W20_J3623_consensus_structure.mrc (#2) coordinates: |
| 8337 | | Matrix rotation and translation |
| 8338 | | 0.99995987 -0.00698720 0.00560699 0.30640707 |
| 8339 | | 0.00699483 0.99997464 -0.00134153 -1.12673692 |
| 8340 | | -0.00559747 0.00138070 0.99998338 0.47678248 |
| 8341 | | Axis 0.15020785 0.61824110 0.77150214 |
| 8342 | | Axis point 136.27032262 49.14800743 0.00000000 |
| 8343 | | Rotation angle (degrees) 0.51919628 |
| 8344 | | Shift along axis -0.28273161 |
| 8345 | | |
| 8346 | | Position of W20_C1_initial_model_v3.pdb L (#47.9) relative to |
| 8347 | | W20_J3623_consensus_structure.mrc (#2) coordinates: |
| 8348 | | Matrix rotation and translation |
| 8349 | | 0.99995987 -0.00698720 0.00560699 0.30640707 |
| 8350 | | 0.00699483 0.99997464 -0.00134153 -1.12673692 |
| 8351 | | -0.00559747 0.00138070 0.99998338 0.47678248 |
| 8352 | | Axis 0.15020785 0.61824110 0.77150214 |
| 8353 | | Axis point 136.27032262 49.14800743 0.00000000 |
| 8354 | | Rotation angle (degrees) 0.51919628 |
| 8355 | | Shift along axis -0.28273161 |
| 8356 | | |
| 8357 | | |
| 8358 | | > fitmap #47.1 inMap #2 |
| 8359 | | |
| 8360 | | Fit molecule W20_C1_initial_model_v3.pdb D (#47.1) to map |
| 8361 | | W20_J3623_consensus_structure.mrc (#2) using 4763 atoms |
| 8362 | | average map value = 0.3391, steps = 60 |
| 8363 | | shifted from previous position = 0.277 |
| 8364 | | rotated from previous position = 0.317 degrees |
| 8365 | | atoms outside contour = 1269, contour level = 0.25746 |
| 8366 | | |
| 8367 | | Position of W20_C1_initial_model_v3.pdb D (#47.1) relative to |
| 8368 | | W20_J3623_consensus_structure.mrc (#2) coordinates: |
| 8369 | | Matrix rotation and translation |
| 8370 | | 0.99993645 -0.00554192 0.00981798 -0.36993206 |
| 8371 | | 0.00552327 0.99998289 0.00192574 -1.44337504 |
| 8372 | | -0.00982849 -0.00187139 0.99994995 1.45151156 |
| 8373 | | Axis -0.16606190 0.85921136 0.48392075 |
| 8374 | | Axis point 170.89777041 0.00000000 46.84174593 |
| 8375 | | Rotation angle (degrees) 0.65506824 |
| 8376 | | Shift along axis -0.47631604 |
| 8377 | | |
| 8378 | | |
| 8379 | | > fitmap #47.2 inMap #2 |
| 8380 | | |
| 8381 | | Fit molecule W20_C1_initial_model_v3.pdb E (#47.2) to map |
| 8382 | | W20_J3623_consensus_structure.mrc (#2) using 1856 atoms |
| 8383 | | average map value = 0.298, steps = 52 |
| 8384 | | shifted from previous position = 0.74 |
| 8385 | | rotated from previous position = 0.516 degrees |
| 8386 | | atoms outside contour = 807, contour level = 0.25746 |
| 8387 | | |
| 8388 | | Position of W20_C1_initial_model_v3.pdb E (#47.2) relative to |
| 8389 | | W20_J3623_consensus_structure.mrc (#2) coordinates: |
| 8390 | | Matrix rotation and translation |
| 8391 | | 0.99985158 -0.01068491 0.01351472 -0.71801951 |
| 8392 | | 0.01073315 0.99993626 -0.00350238 -1.71551668 |
| 8393 | | -0.01347643 0.00364691 0.99990254 2.22722625 |
| 8394 | | Axis 0.20315994 0.76700206 0.60863280 |
| 8395 | | Axis point 169.67451772 0.00000000 53.39691400 |
| 8396 | | Rotation angle (degrees) 1.00818411 |
| 8397 | | Shift along axis -0.10611466 |
| 8398 | | |
| 8399 | | |
| 8400 | | > fitmap #47.3 inMap #2 |
| 8401 | | |
| 8402 | | Fit molecule W20_C1_initial_model_v3.pdb F (#47.3) to map |
| 8403 | | W20_J3623_consensus_structure.mrc (#2) using 7214 atoms |
| 8404 | | average map value = 0.1919, steps = 56 |
| 8405 | | shifted from previous position = 0.863 |
| 8406 | | rotated from previous position = 1.3 degrees |
| 8407 | | atoms outside contour = 5840, contour level = 0.25746 |
| 8408 | | |
| 8409 | | Position of W20_C1_initial_model_v3.pdb F (#47.3) relative to |
| 8410 | | W20_J3623_consensus_structure.mrc (#2) coordinates: |
| 8411 | | Matrix rotation and translation |
| 8412 | | 0.99983282 -0.01767194 -0.00469422 4.64975550 |
| 8413 | | 0.01774465 0.99971568 0.01592750 -6.16380107 |
| 8414 | | 0.00441141 -0.01600813 0.99986213 2.74940793 |
| 8415 | | Axis -0.65778440 -0.18755047 0.72948236 |
| 8416 | | Axis point 350.49529384 263.63816940 0.00000000 |
| 8417 | | Rotation angle (degrees) 1.39100037 |
| 8418 | | Shift along axis 0.10313173 |
| 8419 | | |
| 8420 | | |
| 8421 | | > fitmap #47.4 inMap #2 |
| 8422 | | |
| 8423 | | Fit molecule W20_C1_initial_model_v3.pdb G (#47.4) to map |
| 8424 | | W20_J3623_consensus_structure.mrc (#2) using 1872 atoms |
| 8425 | | average map value = 0.2684, steps = 48 |
| 8426 | | shifted from previous position = 0.694 |
| 8427 | | rotated from previous position = 1.52 degrees |
| 8428 | | atoms outside contour = 902, contour level = 0.25746 |
| 8429 | | |
| 8430 | | Position of W20_C1_initial_model_v3.pdb G (#47.4) relative to |
| 8431 | | W20_J3623_consensus_structure.mrc (#2) coordinates: |
| 8432 | | Matrix rotation and translation |
| 8433 | | 0.99999905 0.00004391 -0.00137935 0.08316312 |
| 8434 | | -0.00001180 0.99972908 0.02327568 -3.69665287 |
| 8435 | | 0.00138000 -0.02327564 0.99972813 3.29847865 |
| 8436 | | Axis -0.99824711 -0.05917154 -0.00119461 |
| 8437 | | Axis point 0.00000000 139.85035535 160.11317726 |
| 8438 | | Rotation angle (degrees) 1.33605990 |
| 8439 | | Shift along axis 0.13177890 |
| 8440 | | |
| 8441 | | |
| 8442 | | > fitmap #47.5 inMap #2 |
| 8443 | | |
| 8444 | | Fit molecule W20_C1_initial_model_v3.pdb H (#47.5) to map |
| 8445 | | W20_J3623_consensus_structure.mrc (#2) using 4752 atoms |
| 8446 | | average map value = 0.2052, steps = 56 |
| 8447 | | shifted from previous position = 0.558 |
| 8448 | | rotated from previous position = 2.67 degrees |
| 8449 | | atoms outside contour = 3635, contour level = 0.25746 |
| 8450 | | |
| 8451 | | Position of W20_C1_initial_model_v3.pdb H (#47.5) relative to |
| 8452 | | W20_J3623_consensus_structure.mrc (#2) coordinates: |
| 8453 | | Matrix rotation and translation |
| 8454 | | 0.99873328 -0.02416117 0.04413700 -1.58450683 |
| 8455 | | 0.02335616 0.99955296 0.01866451 -4.67495439 |
| 8456 | | -0.04456822 -0.01760999 0.99885112 6.98195313 |
| 8457 | | Axis -0.33911247 0.82926157 0.44421615 |
| 8458 | | Axis point 157.93980007 0.00000000 42.26069084 |
| 8459 | | Rotation angle (degrees) 3.06589704 |
| 8460 | | Shift along axis -0.23793767 |
| 8461 | | |
| 8462 | | |
| 8463 | | > fitmap #47.6 inMap #2 |
| 8464 | | |
| 8465 | | Fit molecule W20_C1_initial_model_v3.pdb I (#47.6) to map |
| 8466 | | W20_J3623_consensus_structure.mrc (#2) using 2488 atoms |
| 8467 | | average map value = 0.3942, steps = 48 |
| 8468 | | shifted from previous position = 0.0985 |
| 8469 | | rotated from previous position = 0.351 degrees |
| 8470 | | atoms outside contour = 688, contour level = 0.25746 |
| 8471 | | |
| 8472 | | Position of W20_C1_initial_model_v3.pdb I (#47.6) relative to |
| 8473 | | W20_J3623_consensus_structure.mrc (#2) coordinates: |
| 8474 | | Matrix rotation and translation |
| 8475 | | 0.99997623 -0.00687548 -0.00051167 1.19535071 |
| 8476 | | 0.00687477 0.99997543 -0.00137176 -1.17275950 |
| 8477 | | 0.00052109 0.00136821 0.99999893 -0.25085656 |
| 8478 | | Axis 0.19489694 -0.07346086 0.97806886 |
| 8479 | | Axis point 170.41255683 171.17704205 0.00000000 |
| 8480 | | Rotation angle (degrees) 0.40275148 |
| 8481 | | Shift along axis 0.07376713 |
| 8482 | | |
| 8483 | | |
| 8484 | | > fitmap #47.7 inMap #2 |
| 8485 | | |
| 8486 | | Fit molecule W20_C1_initial_model_v3.pdb J (#47.7) to map |
| 8487 | | W20_J3623_consensus_structure.mrc (#2) using 2879 atoms |
| 8488 | | average map value = 0.2974, steps = 40 |
| 8489 | | shifted from previous position = 0.0947 |
| 8490 | | rotated from previous position = 0.56 degrees |
| 8491 | | atoms outside contour = 1205, contour level = 0.25746 |
| 8492 | | |
| 8493 | | Position of W20_C1_initial_model_v3.pdb J (#47.7) relative to |
| 8494 | | W20_J3623_consensus_structure.mrc (#2) coordinates: |
| 8495 | | Matrix rotation and translation |
| 8496 | | 0.99998391 0.00266199 0.00500985 -1.32620675 |
| 8497 | | -0.00264770 0.99999241 -0.00285677 0.77420871 |
| 8498 | | -0.00501741 0.00284346 0.99998337 0.17173811 |
| 8499 | | Axis 0.44891807 0.78969202 -0.41816155 |
| 8500 | | Axis point 28.71795251 0.00000000 259.81340923 |
| 8501 | | Rotation angle (degrees) 0.36376436 |
| 8502 | | Shift along axis -0.05578601 |
| 8503 | | |
| 8504 | | |
| 8505 | | > fitmap #47.8 inMap #2 |
| 8506 | | |
| 8507 | | Fit molecule W20_C1_initial_model_v3.pdb K (#47.8) to map |
| 8508 | | W20_J3623_consensus_structure.mrc (#2) using 1681 atoms |
| 8509 | | average map value = 0.2582, steps = 2000 |
| 8510 | | shifted from previous position = 3.05 |
| 8511 | | rotated from previous position = 19.1 degrees |
| 8512 | | atoms outside contour = 877, contour level = 0.25746 |
| 8513 | | |
| 8514 | | Position of W20_C1_initial_model_v3.pdb K (#47.8) relative to |
| 8515 | | W20_J3623_consensus_structure.mrc (#2) coordinates: |
| 8516 | | Matrix rotation and translation |
| 8517 | | 0.94200137 -0.31946272 0.10284450 49.35608426 |
| 8518 | | 0.31956519 0.94743045 0.01592570 -41.58693728 |
| 8519 | | -0.10252568 0.01786349 0.99456995 13.43084868 |
| 8520 | | Axis 0.00288696 0.30596518 0.95203833 |
| 8521 | | Axis point 151.23068995 127.03929522 0.00000000 |
| 8522 | | Rotation angle (degrees) 19.60965973 |
| 8523 | | Shift along axis 0.20501701 |
| 8524 | | |
| 8525 | | |
| 8526 | | > fitmap #47.9 inMap #2 |
| 8527 | | |
| 8528 | | Fit molecule W20_C1_initial_model_v3.pdb L (#47.9) to map |
| 8529 | | W20_J3623_consensus_structure.mrc (#2) using 1200 atoms |
| 8530 | | average map value = 0.225, steps = 104 |
| 8531 | | shifted from previous position = 7.72 |
| 8532 | | rotated from previous position = 16.8 degrees |
| 8533 | | atoms outside contour = 923, contour level = 0.25746 |
| 8534 | | |
| 8535 | | Position of W20_C1_initial_model_v3.pdb L (#47.9) relative to |
| 8536 | | W20_J3623_consensus_structure.mrc (#2) coordinates: |
| 8537 | | Matrix rotation and translation |
| 8538 | | 0.95622273 0.06913903 0.28435522 -32.79890580 |
| 8539 | | -0.07184973 0.99741506 -0.00090016 15.80706570 |
| 8540 | | -0.28368241 -0.01957009 0.95871857 38.27014130 |
| 8541 | | Axis -0.03188330 0.97005801 -0.24077150 |
| 8542 | | Axis point 121.59404774 0.00000000 133.26118376 |
| 8543 | | Rotation angle (degrees) 17.02478724 |
| 8544 | | Shift along axis 7.16514893 |
| 8545 | | |
| 8546 | | |
| 8547 | | > hide #!47 models |
| 8548 | | |
| 8549 | | > show #!48 models |
| 8550 | | |
| 8551 | | > split #48 chains |
| 8552 | | |
| 8553 | | Split W20_C1_initial_model_v3_no_wat_lig.pdb (#48) into 9 models |
| 8554 | | Chain information for W20_C1_initial_model_v3_no_wat_lig.pdb D #48.1 |
| 8555 | | --- |
| 8556 | | Chain | Description |
| 8557 | | D | No description available |
| 8558 | | |
| 8559 | | Chain information for W20_C1_initial_model_v3_no_wat_lig.pdb E #48.2 |
| 8560 | | --- |
| 8561 | | Chain | Description |
| 8562 | | E | No description available |
| 8563 | | |
| 8564 | | Chain information for W20_C1_initial_model_v3_no_wat_lig.pdb F #48.3 |
| 8565 | | --- |
| 8566 | | Chain | Description |
| 8567 | | F | No description available |
| 8568 | | |
| 8569 | | Chain information for W20_C1_initial_model_v3_no_wat_lig.pdb G #48.4 |
| 8570 | | --- |
| 8571 | | Chain | Description |
| 8572 | | G | No description available |
| 8573 | | |
| 8574 | | Chain information for W20_C1_initial_model_v3_no_wat_lig.pdb H #48.5 |
| 8575 | | --- |
| 8576 | | Chain | Description |
| 8577 | | H | No description available |
| 8578 | | |
| 8579 | | Chain information for W20_C1_initial_model_v3_no_wat_lig.pdb I #48.6 |
| 8580 | | --- |
| 8581 | | Chain | Description |
| 8582 | | I | No description available |
| 8583 | | |
| 8584 | | Chain information for W20_C1_initial_model_v3_no_wat_lig.pdb J #48.7 |
| 8585 | | --- |
| 8586 | | Chain | Description |
| 8587 | | J | No description available |
| 8588 | | |
| 8589 | | Chain information for W20_C1_initial_model_v3_no_wat_lig.pdb K #48.8 |
| 8590 | | --- |
| 8591 | | Chain | Description |
| 8592 | | K | No description available |
| 8593 | | |
| 8594 | | Chain information for W20_C1_initial_model_v3_no_wat_lig.pdb L #48.9 |
| 8595 | | --- |
| 8596 | | Chain | Description |
| 8597 | | L | No description available |
| 8598 | | |
| 8599 | | |
| 8600 | | > fitmap #48.9 inMap #2 |
| 8601 | | |
| 8602 | | Fit molecule W20_C1_initial_model_v3_no_wat_lig.pdb L (#48.9) to map |
| 8603 | | W20_J3623_consensus_structure.mrc (#2) using 1200 atoms |
| 8604 | | average map value = 0.225, steps = 104 |
| 8605 | | shifted from previous position = 7.35 |
| 8606 | | rotated from previous position = 17 degrees |
| 8607 | | atoms outside contour = 923, contour level = 0.25746 |
| 8608 | | |
| 8609 | | Position of W20_C1_initial_model_v3_no_wat_lig.pdb L (#48.9) relative to |
| 8610 | | W20_J3623_consensus_structure.mrc (#2) coordinates: |
| 8611 | | Matrix rotation and translation |
| 8612 | | 0.95618362 0.06935484 0.28443415 -32.83561380 |
| 8613 | | -0.07187151 0.99741263 -0.00159273 15.88500353 |
| 8614 | | -0.28380868 -0.01891977 0.95869426 38.21611444 |
| 8615 | | Axis -0.02957914 0.97005222 -0.24108871 |
| 8616 | | Axis point 121.32947981 0.00000000 133.38706461 |
| 8617 | | Rotation angle (degrees) 17.03122986 |
| 8618 | | Shift along axis 7.16705836 |
| 8619 | | |
| 8620 | | |
| 8621 | | > fitmap #48.8 inMap #2 |
| 8622 | | |
| 8623 | | Fit molecule W20_C1_initial_model_v3_no_wat_lig.pdb K (#48.8) to map |
| 8624 | | W20_J3623_consensus_structure.mrc (#2) using 1681 atoms |
| 8625 | | average map value = 0.2582, steps = 2000 |
| 8626 | | shifted from previous position = 3 |
| 8627 | | rotated from previous position = 19.6 degrees |
| 8628 | | atoms outside contour = 877, contour level = 0.25746 |
| 8629 | | |
| 8630 | | Position of W20_C1_initial_model_v3_no_wat_lig.pdb K (#48.8) relative to |
| 8631 | | W20_J3623_consensus_structure.mrc (#2) coordinates: |
| 8632 | | Matrix rotation and translation |
| 8633 | | 0.94200226 -0.31945879 0.10284856 49.35474105 |
| 8634 | | 0.31956109 0.94743180 0.01592780 -41.58682474 |
| 8635 | | -0.10253028 0.01786237 0.99456949 13.43178853 |
| 8636 | | Axis 0.00288219 0.30598035 0.95203347 |
| 8637 | | Axis point 151.23135956 127.03695546 0.00000000 |
| 8638 | | Rotation angle (degrees) 19.60950766 |
| 8639 | | Shift along axis 0.20501096 |
| 8640 | | |
| 8641 | | |
| 8642 | | > fitmap #48.7 inMap #2 |
| 8643 | | |
| 8644 | | Fit molecule W20_C1_initial_model_v3_no_wat_lig.pdb J (#48.7) to map |
| 8645 | | W20_J3623_consensus_structure.mrc (#2) using 2879 atoms |
| 8646 | | average map value = 0.2974, steps = 60 |
| 8647 | | shifted from previous position = 0.232 |
| 8648 | | rotated from previous position = 0.38 degrees |
| 8649 | | atoms outside contour = 1205, contour level = 0.25746 |
| 8650 | | |
| 8651 | | Position of W20_C1_initial_model_v3_no_wat_lig.pdb J (#48.7) relative to |
| 8652 | | W20_J3623_consensus_structure.mrc (#2) coordinates: |
| 8653 | | Matrix rotation and translation |
| 8654 | | 0.99998287 0.00312090 0.00495157 -1.40345048 |
| 8655 | | -0.00310539 0.99999025 -0.00313821 0.88665991 |
| 8656 | | -0.00496131 0.00312278 0.99998282 0.10130440 |
| 8657 | | Axis 0.47162964 0.74671971 -0.46901508 |
| 8658 | | Axis point 14.97777640 0.00000000 278.97871593 |
| 8659 | | Rotation angle (degrees) 0.38031035 |
| 8660 | | Shift along axis -0.04733570 |
| 8661 | | |
| 8662 | | |
| 8663 | | > fitmap #48.6 inMap #2 |
| 8664 | | |
| 8665 | | Fit molecule W20_C1_initial_model_v3_no_wat_lig.pdb I (#48.6) to map |
| 8666 | | W20_J3623_consensus_structure.mrc (#2) using 2488 atoms |
| 8667 | | average map value = 0.3942, steps = 68 |
| 8668 | | shifted from previous position = 0.638 |
| 8669 | | rotated from previous position = 0.407 degrees |
| 8670 | | atoms outside contour = 688, contour level = 0.25746 |
| 8671 | | |
| 8672 | | Position of W20_C1_initial_model_v3_no_wat_lig.pdb I (#48.6) relative to |
| 8673 | | W20_J3623_consensus_structure.mrc (#2) coordinates: |
| 8674 | | Matrix rotation and translation |
| 8675 | | 0.99997568 -0.00695350 -0.00053467 1.20906358 |
| 8676 | | 0.00695275 0.99997488 -0.00137680 -1.17749870 |
| 8677 | | 0.00054423 0.00137305 0.99999891 -0.25259458 |
| 8678 | | Axis 0.19342635 -0.07589115 0.97817523 |
| 8679 | | Axis point 169.14439112 171.16881708 0.00000000 |
| 8680 | | Rotation angle (degrees) 0.40727684 |
| 8681 | | Shift along axis 0.07614472 |
| 8682 | | |
| 8683 | | |
| 8684 | | > fitmap #48.5 inMap #2 |
| 8685 | | |
| 8686 | | Fit molecule W20_C1_initial_model_v3_no_wat_lig.pdb H (#48.5) to map |
| 8687 | | W20_J3623_consensus_structure.mrc (#2) using 4752 atoms |
| 8688 | | average map value = 0.2052, steps = 56 |
| 8689 | | shifted from previous position = 0.398 |
| 8690 | | rotated from previous position = 3.04 degrees |
| 8691 | | atoms outside contour = 3633, contour level = 0.25746 |
| 8692 | | |
| 8693 | | Position of W20_C1_initial_model_v3_no_wat_lig.pdb H (#48.5) relative to |
| 8694 | | W20_J3623_consensus_structure.mrc (#2) coordinates: |
| 8695 | | Matrix rotation and translation |
| 8696 | | 0.99875256 -0.02376142 0.04391712 -1.59501966 |
| 8697 | | 0.02297566 0.99956832 0.01831081 -4.59906082 |
| 8698 | | -0.04433325 -0.01727895 0.99886736 6.92788561 |
| 8699 | | Axis -0.33570594 0.83243532 0.44085481 |
| 8700 | | Axis point 157.55390321 0.00000000 42.61926354 |
| 8701 | | Rotation angle (degrees) 3.03852113 |
| 8702 | | Shift along axis -0.23877143 |
| 8703 | | |
| 8704 | | |
| 8705 | | > fitmap #48.4 inMap #2 |
| 8706 | | |
| 8707 | | Fit molecule W20_C1_initial_model_v3_no_wat_lig.pdb G (#48.4) to map |
| 8708 | | W20_J3623_consensus_structure.mrc (#2) using 1872 atoms |
| 8709 | | average map value = 0.2684, steps = 52 |
| 8710 | | shifted from previous position = 0.66 |
| 8711 | | rotated from previous position = 1.34 degrees |
| 8712 | | atoms outside contour = 904, contour level = 0.25746 |
| 8713 | | |
| 8714 | | Position of W20_C1_initial_model_v3_no_wat_lig.pdb G (#48.4) relative to |
| 8715 | | W20_J3623_consensus_structure.mrc (#2) coordinates: |
| 8716 | | Matrix rotation and translation |
| 8717 | | 0.99999668 -0.00055774 -0.00251381 0.32378285 |
| 8718 | | 0.00061619 0.99972807 0.02331118 -3.79263384 |
| 8719 | | 0.00250012 -0.02331266 0.99972510 3.13991886 |
| 8720 | | Axis -0.99395584 -0.10688996 0.02502646 |
| 8721 | | Axis point 0.00000000 132.58526090 163.49903175 |
| 8722 | | Rotation angle (degrees) 1.34391993 |
| 8723 | | Shift along axis 0.16214970 |
| 8724 | | |
| 8725 | | |
| 8726 | | > fitmap #48.3 inMap #2 |
| 8727 | | |
| 8728 | | Fit molecule W20_C1_initial_model_v3_no_wat_lig.pdb F (#48.3) to map |
| 8729 | | W20_J3623_consensus_structure.mrc (#2) using 7214 atoms |
| 8730 | | average map value = 0.1919, steps = 52 |
| 8731 | | shifted from previous position = 1.21 |
| 8732 | | rotated from previous position = 1.38 degrees |
| 8733 | | atoms outside contour = 5841, contour level = 0.25746 |
| 8734 | | |
| 8735 | | Position of W20_C1_initial_model_v3_no_wat_lig.pdb F (#48.3) relative to |
| 8736 | | W20_J3623_consensus_structure.mrc (#2) coordinates: |
| 8737 | | Matrix rotation and translation |
| 8738 | | 0.99983183 -0.01777810 -0.00449952 4.61473624 |
| 8739 | | 0.01784647 0.99971839 0.01564141 -6.12537351 |
| 8740 | | 0.00422018 -0.01571908 0.99986754 2.73144245 |
| 8741 | | Axis -0.64988004 -0.18069744 0.73824411 |
| 8742 | | Axis point 346.08368056 260.84380532 -0.00000000 |
| 8743 | | Rotation angle (degrees) 1.38256144 |
| 8744 | | Shift along axis 0.12428563 |
| 8745 | | |
| 8746 | | |
| 8747 | | > fitmap #48.2 inMap #2 |
| 8748 | | |
| 8749 | | Fit molecule W20_C1_initial_model_v3_no_wat_lig.pdb E (#48.2) to map |
| 8750 | | W20_J3623_consensus_structure.mrc (#2) using 1856 atoms |
| 8751 | | average map value = 0.298, steps = 48 |
| 8752 | | shifted from previous position = 0.513 |
| 8753 | | rotated from previous position = 0.756 degrees |
| 8754 | | atoms outside contour = 811, contour level = 0.25746 |
| 8755 | | |
| 8756 | | Position of W20_C1_initial_model_v3_no_wat_lig.pdb E (#48.2) relative to |
| 8757 | | W20_J3623_consensus_structure.mrc (#2) coordinates: |
| 8758 | | Matrix rotation and translation |
| 8759 | | 0.99991922 -0.00558717 0.01141652 -1.06360279 |
| 8760 | | 0.00562764 0.99997799 -0.00351551 -0.81089288 |
| 8761 | | -0.01139663 0.00357947 0.99992865 1.85466537 |
| 8762 | | Axis 0.26882817 0.86438839 0.42492838 |
| 8763 | | Axis point 169.58714833 0.00000000 89.68331202 |
| 8764 | | Rotation angle (degrees) 0.75610423 |
| 8765 | | Shift along axis -0.19875283 |
| 8766 | | |
| 8767 | | |
| 8768 | | > fitmap #48.1 inMap #2 |
| 8769 | | |
| 8770 | | Fit molecule W20_C1_initial_model_v3_no_wat_lig.pdb D (#48.1) to map |
| 8771 | | W20_J3623_consensus_structure.mrc (#2) using 4763 atoms |
| 8772 | | average map value = 0.3391, steps = 52 |
| 8773 | | shifted from previous position = 0.556 |
| 8774 | | rotated from previous position = 0.656 degrees |
| 8775 | | atoms outside contour = 1271, contour level = 0.25746 |
| 8776 | | |
| 8777 | | Position of W20_C1_initial_model_v3_no_wat_lig.pdb D (#48.1) relative to |
| 8778 | | W20_J3623_consensus_structure.mrc (#2) coordinates: |
| 8779 | | Matrix rotation and translation |
| 8780 | | 0.99993657 -0.00557361 0.00978726 -0.36204539 |
| 8781 | | 0.00555366 0.99998245 0.00206434 -1.46156620 |
| 8782 | | -0.00979859 -0.00200985 0.99994997 1.46464333 |
| 8783 | | Axis -0.17797856 0.85559501 0.48608724 |
| 8784 | | Axis point 172.75674812 0.00000000 46.73299778 |
| 8785 | | Rotation angle (degrees) 0.65580741 |
| 8786 | | Shift along axis -0.47412801 |
| 8787 | | |
| 8788 | | |
| 8789 | | > hide #!48 models |
| 8790 | | |
| 8791 | | > show #!49 models |
| 8792 | | |
| 8793 | | > hide #!49 models |
| 8794 | | |
| 8795 | | > show #!50 models |
| 8796 | | |
| 8797 | | > fitmap #50 inMap #2 |
| 8798 | | |
| 8799 | | Fit molecule W20_C1_initial_model_no_wat_lig.pdb (#50) to map |
| 8800 | | W20_J3623_consensus_structure.mrc (#2) using 25890 atoms |
| 8801 | | average map value = 0.2892, steps = 92 |
| 8802 | | shifted from previous position = 6.37 |
| 8803 | | rotated from previous position = 6 degrees |
| 8804 | | atoms outside contour = 13101, contour level = 0.25746 |
| 8805 | | |
| 8806 | | Position of W20_C1_initial_model_no_wat_lig.pdb (#50) relative to |
| 8807 | | W20_J3623_consensus_structure.mrc (#2) coordinates: |
| 8808 | | Matrix rotation and translation |
| 8809 | | -0.92962183 0.23756050 -0.28172373 363.07149255 |
| 8810 | | -0.02176229 -0.79853926 -0.60154921 515.96615164 |
| 8811 | | -0.36787180 -0.55308232 0.74750939 166.87632524 |
| 8812 | | Axis 0.17464117 0.31041809 -0.93442018 |
| 8813 | | Axis point 215.59521690 272.59770300 0.00000000 |
| 8814 | | Rotation angle (degrees) 172.02382323 |
| 8815 | | Shift along axis 67.63985482 |
| 8816 | | |
| 8817 | | |
| 8818 | | > show #51 models |
| 8819 | | |
| 8820 | | > hide #51 models |
| 8821 | | |
| 8822 | | > hide #!50 models |
| 8823 | | |
| 8824 | | > show #!50 models |
| 8825 | | |
| 8826 | | > split #50 chains |
| 8827 | | |
| 8828 | | Split W20_C1_initial_model_no_wat_lig.pdb (#50) into 11 models |
| 8829 | | Chain information for W20_C1_initial_model_no_wat_lig.pdb A0 #50.1 |
| 8830 | | --- |
| 8831 | | Chain | Description |
| 8832 | | A0 | No description available |
| 8833 | | |
| 8834 | | Chain information for W20_C1_initial_model_no_wat_lig.pdb A1 #50.2 |
| 8835 | | --- |
| 8836 | | Chain | Description |
| 8837 | | A1 | No description available |
| 8838 | | |
| 8839 | | Chain information for W20_C1_initial_model_no_wat_lig.pdb A2 #50.3 |
| 8840 | | --- |
| 8841 | | Chain | Description |
| 8842 | | A2 | No description available |
| 8843 | | |
| 8844 | | Chain information for W20_C1_initial_model_no_wat_lig.pdb A3 #50.4 |
| 8845 | | --- |
| 8846 | | Chain | Description |
| 8847 | | A3 | No description available |
| 8848 | | |
| 8849 | | Chain information for W20_C1_initial_model_no_wat_lig.pdb A4 #50.5 |
| 8850 | | --- |
| 8851 | | Chain | Description |
| 8852 | | A4 | No description available |
| 8853 | | |
| 8854 | | Chain information for W20_C1_initial_model_no_wat_lig.pdb A5 #50.6 |
| 8855 | | --- |
| 8856 | | Chain | Description |
| 8857 | | A5 | No description available |
| 8858 | | |
| 8859 | | Chain information for W20_C1_initial_model_no_wat_lig.pdb A6 #50.7 |
| 8860 | | --- |
| 8861 | | Chain | Description |
| 8862 | | A6 | No description available |
| 8863 | | |
| 8864 | | Chain information for W20_C1_initial_model_no_wat_lig.pdb A7 #50.8 |
| 8865 | | --- |
| 8866 | | Chain | Description |
| 8867 | | A7 | No description available |
| 8868 | | |
| 8869 | | Chain information for W20_C1_initial_model_no_wat_lig.pdb A8 #50.9 |
| 8870 | | --- |
| 8871 | | Chain | Description |
| 8872 | | A8 | No description available |
| 8873 | | |
| 8874 | | Chain information for W20_C1_initial_model_no_wat_lig.pdb A9 #50.10 |
| 8875 | | --- |
| 8876 | | Chain | Description |
| 8877 | | A9 | No description available |
| 8878 | | |
| 8879 | | Chain information for W20_C1_initial_model_no_wat_lig.pdb BA #50.11 |
| 8880 | | --- |
| 8881 | | Chain | Description |
| 8882 | | BA | No description available |
| 8883 | | |
| 8884 | | |
| 8885 | | > hide #50.1 models |
| 8886 | | |
| 8887 | | > show #50.1 models |
| 8888 | | |
| 8889 | | > hide #50.1 models |
| 8890 | | |
| 8891 | | > show #50.1 models |
| 8892 | | |
| 8893 | | > hide #!50.2 models |
| 8894 | | |
| 8895 | | > show #!50.2 models |
| 8896 | | |
| 8897 | | > hide #!50.2 models |
| 8898 | | |
| 8899 | | > show #!50.2 models |
| 8900 | | |
| 8901 | | > hide #!50.3 models |
| 8902 | | |
| 8903 | | > show #!50.3 models |
| 8904 | | |
| 8905 | | > hide #!50.4 models |
| 8906 | | |
| 8907 | | > show #!50.4 models |
| 8908 | | |
| 8909 | | > hide #!50.3 models |
| 8910 | | |
| 8911 | | > show #!50.3 models |
| 8912 | | |
| 8913 | | > ui tool show Matchmaker |
| 8914 | | |
| 8915 | | > matchmaker #51 to #50.3 |
| 8916 | | |
| 8917 | | Computing secondary structure |
| 8918 | | Parameters |
| 8919 | | --- |
| 8920 | | Chain pairing | bb |
| 8921 | | Alignment algorithm | Needleman-Wunsch |
| 8922 | | Similarity matrix | BLOSUM-62 |
| 8923 | | SS fraction | 0.3 |
| 8924 | | Gap open (HH/SS/other) | 18/18/6 |
| 8925 | | Gap extend | 1 |
| 8926 | | SS matrix | | | H | S | O |
| 8927 | | ---|---|---|--- |
| 8928 | | H | 6 | -9 | -6 |
| 8929 | | S | | 6 | -6 |
| 8930 | | O | | | 4 |
| 8931 | | Iteration cutoff | 2 |
| 8932 | | |
| 8933 | | Matchmaker W20_C1_initial_model_no_wat_lig.pdb A2, chain A2 (#50.3) with |
| 8934 | | A_fixed_model_2_A.pdb, chain A (#51), sequence alignment score = 2192.7 |
| 8935 | | RMSD between 285 pruned atom pairs is 0.343 angstroms; (across all 445 pairs: |
| 8936 | | 7.570) |
| 8937 | | |
| 8938 | | |
| 8939 | | > show #51 models |
| 8940 | | |
| 8941 | | > hide #!50.7 models |
| 8942 | | |
| 8943 | | > show #!50.7 models |
| 8944 | | |
| 8945 | | > ui tool show Matchmaker |
| 8946 | | |
| 8947 | | > show #52 models |
| 8948 | | |
| 8949 | | > hide #52 models |
| 8950 | | |
| 8951 | | > show #!53 models |
| 8952 | | |
| 8953 | | > matchmaker #!53 to #50.7 |
| 8954 | | |
| 8955 | | Computing secondary structure |
| 8956 | | Parameters |
| 8957 | | --- |
| 8958 | | Chain pairing | bb |
| 8959 | | Alignment algorithm | Needleman-Wunsch |
| 8960 | | Similarity matrix | BLOSUM-62 |
| 8961 | | SS fraction | 0.3 |
| 8962 | | Gap open (HH/SS/other) | 18/18/6 |
| 8963 | | Gap extend | 1 |
| 8964 | | SS matrix | | | H | S | O |
| 8965 | | ---|---|---|--- |
| 8966 | | H | 6 | -9 | -6 |
| 8967 | | S | | 6 | -6 |
| 8968 | | O | | | 4 |
| 8969 | | Iteration cutoff | 2 |
| 8970 | | |
| 8971 | | Matchmaker W20_C1_initial_model_no_wat_lig.pdb A6, chain A6 (#50.7) with |
| 8972 | | C1_fixed_model_2_C1.pdb, chain C1 (#53), sequence alignment score = 1136.3 |
| 8973 | | RMSD between 188 pruned atom pairs is 0.567 angstroms; (across all 446 pairs: |
| 8974 | | 42.070) |
| 8975 | | |
| 8976 | | |
| 8977 | | > hide #!53 models |
| 8978 | | |
| 8979 | | > show #!54 models |
| 8980 | | |
| 8981 | | > hide #!50.8 models |
| 8982 | | |
| 8983 | | > show #!50.8 models |
| 8984 | | |
| 8985 | | > hide #!50.8 models |
| 8986 | | |
| 8987 | | > show #!50.8 models |
| 8988 | | |
| 8989 | | > ui tool show Matchmaker |
| 8990 | | |
| 8991 | | > hide #50.6 models |
| 8992 | | |
| 8993 | | > show #50.6 models |
| 8994 | | |
| 8995 | | > hide #!50.4 models |
| 8996 | | |
| 8997 | | > show #!50.4 models |
| 8998 | | |
| 8999 | | > matchmaker #52 to #50.4 |
| 9000 | | |
| 9001 | | Computing secondary structure |
| 9002 | | Parameters |
| 9003 | | --- |
| 9004 | | Chain pairing | bb |
| 9005 | | Alignment algorithm | Needleman-Wunsch |
| 9006 | | Similarity matrix | BLOSUM-62 |
| 9007 | | SS fraction | 0.3 |
| 9008 | | Gap open (HH/SS/other) | 18/18/6 |
| 9009 | | Gap extend | 1 |
| 9010 | | SS matrix | | | H | S | O |
| 9011 | | ---|---|---|--- |
| 9012 | | H | 6 | -9 | -6 |
| 9013 | | S | | 6 | -6 |
| 9014 | | O | | | 4 |
| 9015 | | Iteration cutoff | 2 |
| 9016 | | |
| 9017 | | Matchmaker W20_C1_initial_model_no_wat_lig.pdb A3, chain A3 (#50.4) with |
| 9018 | | B_fixed_model_2_B.pdb, chain B (#52), sequence alignment score = 2854.3 |
| 9019 | | RMSD between 172 pruned atom pairs is 0.254 angstroms; (across all 567 pairs: |
| 9020 | | 4.295) |
| 9021 | | |
| 9022 | | |
| 9023 | | > show #52 models |
| 9024 | | |
| 9025 | | > hide #52 models |
| 9026 | | |
| 9027 | | > ui tool show Matchmaker |
| 9028 | | |
| 9029 | | > matchmaker #!54 to #50.8 |
| 9030 | | |
| 9031 | | Computing secondary structure |
| 9032 | | Parameters |
| 9033 | | --- |
| 9034 | | Chain pairing | bb |
| 9035 | | Alignment algorithm | Needleman-Wunsch |
| 9036 | | Similarity matrix | BLOSUM-62 |
| 9037 | | SS fraction | 0.3 |
| 9038 | | Gap open (HH/SS/other) | 18/18/6 |
| 9039 | | Gap extend | 1 |
| 9040 | | SS matrix | | | H | S | O |
| 9041 | | ---|---|---|--- |
| 9042 | | H | 6 | -9 | -6 |
| 9043 | | S | | 6 | -6 |
| 9044 | | O | | | 4 |
| 9045 | | Iteration cutoff | 2 |
| 9046 | | |
| 9047 | | Matchmaker W20_C1_initial_model_no_wat_lig.pdb A7, chain A7 (#50.8) with |
| 9048 | | D1_fixed_model_2_D1.pdb, chain D1 (#54), sequence alignment score = 660 |
| 9049 | | RMSD between 129 pruned atom pairs is 0.312 angstroms; (across all 131 pairs: |
| 9050 | | 0.453) |
| 9051 | | |
| 9052 | | |
| 9053 | | > hide #!54 models |
| 9054 | | |
| 9055 | | > show #!55 models |
| 9056 | | |
| 9057 | | > hide #!55 models |
| 9058 | | |
| 9059 | | > show #!55 models |
| 9060 | | |
| 9061 | | > hide #!50.10 models |
| 9062 | | |
| 9063 | | > show #!50.10 models |
| 9064 | | |
| 9065 | | > hide #!50.10 models |
| 9066 | | |
| 9067 | | > show #!50.10 models |
| 9068 | | |
| 9069 | | > ui tool show Matchmaker |
| 9070 | | |
| 9071 | | > matchmaker #!55 to #50.10 |
| 9072 | | |
| 9073 | | Computing secondary structure |
| 9074 | | Parameters |
| 9075 | | --- |
| 9076 | | Chain pairing | bb |
| 9077 | | Alignment algorithm | Needleman-Wunsch |
| 9078 | | Similarity matrix | BLOSUM-62 |
| 9079 | | SS fraction | 0.3 |
| 9080 | | Gap open (HH/SS/other) | 18/18/6 |
| 9081 | | Gap extend | 1 |
| 9082 | | SS matrix | | | H | S | O |
| 9083 | | ---|---|---|--- |
| 9084 | | H | 6 | -9 | -6 |
| 9085 | | S | | 6 | -6 |
| 9086 | | O | | | 4 |
| 9087 | | Iteration cutoff | 2 |
| 9088 | | |
| 9089 | | Matchmaker W20_C1_initial_model_no_wat_lig.pdb A9, chain A9 (#50.10) with |
| 9090 | | E2_full_shifted_sm_1.pdb, chain E2 (#55), sequence alignment score = 1394.2 |
| 9091 | | RMSD between 277 pruned atom pairs is 0.670 angstroms; (across all 277 pairs: |
| 9092 | | 0.670) |
| 9093 | | |
| 9094 | | |
| 9095 | | > hide #!55 models |
| 9096 | | |
| 9097 | | > show #!55 models |
| 9098 | | |
| 9099 | | > hide #!55 models |
| 9100 | | |
| 9101 | | > show #!55 models |
| 9102 | | |
| 9103 | | > hide #!50.10 models |
| 9104 | | |
| 9105 | | > show #!50.10 models |
| 9106 | | |
| 9107 | | > hide #!55 models |
| 9108 | | |
| 9109 | | > show #56 models |
| 9110 | | |
| 9111 | | > hide #50.11 models |
| 9112 | | |
| 9113 | | > show #50.11 models |
| 9114 | | |
| 9115 | | > ui tool show Matchmaker |
| 9116 | | |
| 9117 | | > matchmaker #56 to #50.11 |
| 9118 | | |
| 9119 | | Computing secondary structure |
| 9120 | | Parameters |
| 9121 | | --- |
| 9122 | | Chain pairing | bb |
| 9123 | | Alignment algorithm | Needleman-Wunsch |
| 9124 | | Similarity matrix | BLOSUM-62 |
| 9125 | | SS fraction | 0.3 |
| 9126 | | Gap open (HH/SS/other) | 18/18/6 |
| 9127 | | Gap extend | 1 |
| 9128 | | SS matrix | | | H | S | O |
| 9129 | | ---|---|---|--- |
| 9130 | | H | 6 | -9 | -6 |
| 9131 | | S | | 6 | -6 |
| 9132 | | O | | | 4 |
| 9133 | | Iteration cutoff | 2 |
| 9134 | | |
| 9135 | | Matchmaker W20_C1_initial_model_no_wat_lig.pdb BA, chain BA (#50.11) with |
| 9136 | | F_fixed_model_2_F.pdb, chain F (#56), sequence alignment score = 725 |
| 9137 | | RMSD between 144 pruned atom pairs is 0.244 angstroms; (across all 144 pairs: |
| 9138 | | 0.244) |
| 9139 | | |
| 9140 | | |
| 9141 | | > hide #56 models |
| 9142 | | |
| 9143 | | > hide #51 models |
| 9144 | | |
| 9145 | | > show #36 models |
| 9146 | | |
| 9147 | | > hide #36 models |
| 9148 | | |
| 9149 | | > show #36 models |
| 9150 | | |
| 9151 | | > hide #36 models |
| 9152 | | |
| 9153 | | > show #36 models |
| 9154 | | |
| 9155 | | > hide #36 models |
| 9156 | | |
| 9157 | | > show #35 models |
| 9158 | | |
| 9159 | | > hide #35 models |
| 9160 | | |
| 9161 | | > show #52 models |
| 9162 | | |
| 9163 | | > fitmap #52 inMap #2 |
| 9164 | | |
| 9165 | | Fit molecule B_fixed_model_2_B.pdb (#52) to map |
| 9166 | | W20_J3623_consensus_structure.mrc (#2) using 7500 atoms |
| 9167 | | average map value = 0.316, steps = 88 |
| 9168 | | shifted from previous position = 2.73 |
| 9169 | | rotated from previous position = 5.83 degrees |
| 9170 | | atoms outside contour = 2940, contour level = 0.25746 |
| 9171 | | |
| 9172 | | Position of B_fixed_model_2_B.pdb (#52) relative to |
| 9173 | | W20_J3623_consensus_structure.mrc (#2) coordinates: |
| 9174 | | Matrix rotation and translation |
| 9175 | | -0.33624269 -0.92088355 -0.19726715 276.34525406 |
| 9176 | | -0.77887628 0.15416253 0.60793557 158.89643495 |
| 9177 | | -0.52942667 0.35806060 -0.76909038 243.62727331 |
| 9178 | | Axis -0.56887772 0.75621078 0.32330079 |
| 9179 | | Axis point 214.85544208 0.00000000 65.79075689 |
| 9180 | | Rotation angle (degrees) 167.31322736 |
| 9181 | | Shift along axis 41.71742887 |
| 9182 | | |
| 9183 | | |
| 9184 | | > fitmap #50.112 |
| 9185 | | |
| 9186 | | Missing required "in_map" argument |
| 9187 | | |
| 9188 | | > fitmap #50.11 inMap #2 |
| 9189 | | |
| 9190 | | Fit molecule W20_C1_initial_model_no_wat_lig.pdb BA (#50.11) to map |
| 9191 | | W20_J3623_consensus_structure.mrc (#2) using 1158 atoms |
| 9192 | | average map value = 0.2425, steps = 216 |
| 9193 | | shifted from previous position = 24 |
| 9194 | | rotated from previous position = 39.5 degrees |
| 9195 | | atoms outside contour = 674, contour level = 0.25746 |
| 9196 | | |
| 9197 | | Position of W20_C1_initial_model_no_wat_lig.pdb BA (#50.11) relative to |
| 9198 | | W20_J3623_consensus_structure.mrc (#2) coordinates: |
| 9199 | | Matrix rotation and translation |
| 9200 | | -0.96490741 -0.23836579 -0.11016098 456.74587096 |
| 9201 | | 0.21274833 -0.46373391 -0.86005174 403.93698444 |
| 9202 | | 0.15392153 -0.85330687 0.49817221 176.52114975 |
| 9203 | | Axis 0.01290218 -0.50515953 0.86292954 |
| 9204 | | Axis point 197.70794601 288.88132577 0.00000000 |
| 9205 | | Rotation angle (degrees) 164.84772556 |
| 9206 | | Shift along axis -45.83428628 |
| 9207 | | |
| 9208 | | |
| 9209 | | > show #56 models |
| 9210 | | |
| 9211 | | > hide #56 models |
| 9212 | | |
| 9213 | | > show #56 models |
| 9214 | | |
| 9215 | | > hide #56 models |
| 9216 | | |
| 9217 | | > ui tool show Matchmaker |
| 9218 | | |
| 9219 | | > matchmaker #50.11 to #56 |
| 9220 | | |
| 9221 | | Computing secondary structure |
| 9222 | | Parameters |
| 9223 | | --- |
| 9224 | | Chain pairing | bb |
| 9225 | | Alignment algorithm | Needleman-Wunsch |
| 9226 | | Similarity matrix | BLOSUM-62 |
| 9227 | | SS fraction | 0.3 |
| 9228 | | Gap open (HH/SS/other) | 18/18/6 |
| 9229 | | Gap extend | 1 |
| 9230 | | SS matrix | | | H | S | O |
| 9231 | | ---|---|---|--- |
| 9232 | | H | 6 | -9 | -6 |
| 9233 | | S | | 6 | -6 |
| 9234 | | O | | | 4 |
| 9235 | | Iteration cutoff | 2 |
| 9236 | | |
| 9237 | | Matchmaker F_fixed_model_2_F.pdb, chain F (#56) with |
| 9238 | | W20_C1_initial_model_no_wat_lig.pdb BA, chain BA (#50.11), sequence alignment |
| 9239 | | score = 725 |
| 9240 | | RMSD between 144 pruned atom pairs is 0.244 angstroms; (across all 144 pairs: |
| 9241 | | 0.244) |
| 9242 | | |
| 9243 | | |
| 9244 | | > save "/Users/becca/Desktop/Postdoc/COPI notebooks and |
| 9245 | | > resources/Processing/W20_MM_cyto_COPI/20250704_W20_COPI_cyto_alignments.cxs" |
| 9246 | | |
| 9247 | | > hide #!2 models |
| 9248 | | |
| 9249 | | > hide #!50 models |
| 9250 | | |
| 9251 | | > show #!50 models |
| 9252 | | |
| 9253 | | > hide #52 models |
| 9254 | | |
| 9255 | | > show #!53 models |
| 9256 | | |
| 9257 | | > hide #!53 models |
| 9258 | | |
| 9259 | | > show #!54 models |
| 9260 | | |
| 9261 | | > hide #!54 models |
| 9262 | | |
| 9263 | | > show #!54 models |
| 9264 | | |
| 9265 | | > hide #!54 models |
| 9266 | | |
| 9267 | | > show #!55 models |
| 9268 | | |
| 9269 | | > hide #!55 models |
| 9270 | | |
| 9271 | | > show #52 models |
| 9272 | | |
| 9273 | | > show #!33 models |
| 9274 | | |
| 9275 | | > color #33 #b2b2b290 models |
| 9276 | | |
| 9277 | | > color #33 #b2b2b28f models |
| 9278 | | |
| 9279 | | > hide #52 models |
| 9280 | | |
| 9281 | | > show #52 models |
| 9282 | | |
| 9283 | | > hide #!50.8 models |
| 9284 | | |
| 9285 | | > show #!50.8 models |
| 9286 | | |
| 9287 | | > hide #50.6 models |
| 9288 | | |
| 9289 | | > show #50.6 models |
| 9290 | | |
| 9291 | | > hide #!50.4 models |
| 9292 | | |
| 9293 | | > hide #!50.8 models |
| 9294 | | |
| 9295 | | > show #!50.8 models |
| 9296 | | |
| 9297 | | > fitmap #50.8 inMap #2 |
| 9298 | | |
| 9299 | | Fit molecule W20_C1_initial_model_no_wat_lig.pdb A7 (#50.8) to map |
| 9300 | | W20_J3623_consensus_structure.mrc (#2) using 1057 atoms |
| 9301 | | average map value = 0.2692, steps = 56 |
| 9302 | | shifted from previous position = 0.944 |
| 9303 | | rotated from previous position = 4.88 degrees |
| 9304 | | atoms outside contour = 542, contour level = 0.25746 |
| 9305 | | |
| 9306 | | Position of W20_C1_initial_model_no_wat_lig.pdb A7 (#50.8) relative to |
| 9307 | | W20_J3623_consensus_structure.mrc (#2) coordinates: |
| 9308 | | Matrix rotation and translation |
| 9309 | | -0.93105828 0.16643564 -0.32469934 390.48149308 |
| 9310 | | 0.06181231 -0.80509185 -0.58992064 497.62568127 |
| 9311 | | -0.35959661 -0.56932091 0.73929966 170.58533102 |
| 9312 | | Axis 0.18360289 0.31103515 -0.93249510 |
| 9313 | | Axis point 219.20897097 271.57117220 0.00000000 |
| 9314 | | Rotation angle (degrees) 176.78409911 |
| 9315 | | Shift along axis 67.40262593 |
| 9316 | | |
| 9317 | | Must specify one map, got 4 |
| 9318 | | |
| 9319 | | > hide #!50.8 models |
| 9320 | | |
| 9321 | | > show #!50.8 models |
| 9322 | | |
| 9323 | | > hide #!33 models |
| 9324 | | |
| 9325 | | > show #!46 models |
| 9326 | | |
| 9327 | | > show #!33 models |
| 9328 | | |
| 9329 | | > hide #!46 models |
| 9330 | | |
| 9331 | | > hide #!33.4 models |
| 9332 | | |
| 9333 | | > show #!33.4 models |
| 9334 | | |
| 9335 | | > hide #!33.3 models |
| 9336 | | |
| 9337 | | > show #!33.3 models |
| 9338 | | |
| 9339 | | > hide #!33.2 models |
| 9340 | | |
| 9341 | | > show #!33.2 models |
| 9342 | | |
| 9343 | | > hide #!33.1 models |
| 9344 | | |
| 9345 | | > show #!33.1 models |
| 9346 | | |
| 9347 | | > fitmap #50.8 inMap #33.1 |
| 9348 | | |
| 9349 | | Fit molecule W20_C1_initial_model_no_wat_lig.pdb A7 (#50.8) to map |
| 9350 | | W20_J160_run_ct6_body001.mrc (#33.1) using 1057 atoms |
| 9351 | | average map value = 0.01137, steps = 52 |
| 9352 | | shifted from previous position = 0.606 |
| 9353 | | rotated from previous position = 3.76 degrees |
| 9354 | | atoms outside contour = 692, contour level = 0.014811 |
| 9355 | | |
| 9356 | | Position of W20_C1_initial_model_no_wat_lig.pdb A7 (#50.8) relative to |
| 9357 | | W20_J160_run_ct6_body001.mrc (#33.1) coordinates: |
| 9358 | | Matrix rotation and translation |
| 9359 | | -0.93056714 0.15394973 -0.33218109 390.44774590 |
| 9360 | | 0.14816644 -0.67132819 -0.72619912 477.44064772 |
| 9361 | | -0.33480069 -0.72499513 0.60190577 233.60614941 |
| 9362 | | Axis 0.18631674 0.40538097 -0.89495941 |
| 9363 | | Axis point 219.95045580 291.28468198 0.00000000 |
| 9364 | | Rotation angle (degrees) 179.81487490 |
| 9365 | | Shift along axis 57.22428050 |
| 9366 | | |
| 9367 | | |
| 9368 | | > hide #!50.8 models |
| 9369 | | |
| 9370 | | > show #!50.8 models |
| 9371 | | |
| 9372 | | > hide #50.9 models |
| 9373 | | |
| 9374 | | > show #50.9 models |
| 9375 | | |
| 9376 | | > hide #!50.8 models |
| 9377 | | |
| 9378 | | > show #!50.8 models |
| 9379 | | |
| 9380 | | > select add #50.8 |
| 9381 | | |
| 9382 | | 1057 atoms, 1072 bonds, 1 pseudobond, 131 residues, 2 models selected |
| 9383 | | |
| 9384 | | > view matrix models |
| 9385 | | > #50.8,-0.90846,0.21715,-0.35712,384.12,0.037606,-0.80851,-0.58728,501.46,-0.41627,-0.54695,0.72634,175.24 |
| 9386 | | |
| 9387 | | > ui mousemode right "rotate selected models" |
| 9388 | | |
| 9389 | | > view matrix models |
| 9390 | | > #50.8,-0.98593,-0.16588,0.020652,396.52,0.059686,-0.46474,-0.88344,489.6,0.15614,-0.86977,0.4681,195.56 |
| 9391 | | |
| 9392 | | > fitmap #50.8 inMap #33.1 |
| 9393 | | |
| 9394 | | Fit molecule W20_C1_initial_model_no_wat_lig.pdb A7 (#50.8) to map |
| 9395 | | W20_J160_run_ct6_body001.mrc (#33.1) using 1057 atoms |
| 9396 | | average map value = 0.01486, steps = 88 |
| 9397 | | shifted from previous position = 2.12 |
| 9398 | | rotated from previous position = 17.4 degrees |
| 9399 | | atoms outside contour = 497, contour level = 0.014811 |
| 9400 | | |
| 9401 | | Position of W20_C1_initial_model_no_wat_lig.pdb A7 (#50.8) relative to |
| 9402 | | W20_J160_run_ct6_body001.mrc (#33.1) coordinates: |
| 9403 | | Matrix rotation and translation |
| 9404 | | -0.98685430 0.03145837 0.15852120 311.01536433 |
| 9405 | | -0.16062302 -0.29929277 -0.94054457 509.24173521 |
| 9406 | | 0.01785625 -0.95364260 0.30041129 284.21437212 |
| 9407 | | Axis -0.05493227 0.58993953 -0.80557668 |
| 9408 | | Axis point 163.53469015 347.02960176 0.00000000 |
| 9409 | | Rotation angle (degrees) 173.15292285 |
| 9410 | | Shift along axis 54.38058021 |
| 9411 | | |
| 9412 | | |
| 9413 | | > select subtract #50.8 |
| 9414 | | |
| 9415 | | Nothing selected |
| 9416 | | |
| 9417 | | > fitmap #50.7 inMap #33.1 |
| 9418 | | |
| 9419 | | Fit molecule W20_C1_initial_model_no_wat_lig.pdb A6 (#50.7) to map |
| 9420 | | W20_J160_run_ct6_body001.mrc (#33.1) using 4264 atoms |
| 9421 | | average map value = 0.005768, steps = 160 |
| 9422 | | shifted from previous position = 2.43 |
| 9423 | | rotated from previous position = 3.77 degrees |
| 9424 | | atoms outside contour = 3553, contour level = 0.014811 |
| 9425 | | |
| 9426 | | Position of W20_C1_initial_model_no_wat_lig.pdb A6 (#50.7) relative to |
| 9427 | | W20_J160_run_ct6_body001.mrc (#33.1) coordinates: |
| 9428 | | Matrix rotation and translation |
| 9429 | | -0.95014016 0.15038437 -0.27316336 382.66549579 |
| 9430 | | 0.08966442 -0.70725915 -0.70124517 487.55197868 |
| 9431 | | -0.29865360 -0.69077423 0.65851119 202.15694868 |
| 9432 | | Axis 0.15703122 0.38227356 -0.91060865 |
| 9433 | | Axis point 213.00898151 282.79480162 0.00000000 |
| 9434 | | Rotation angle (degrees) 178.08938676 |
| 9435 | | Shift along axis 62.38279471 |
| 9436 | | |
| 9437 | | |
| 9438 | | > hide #50.11 models |
| 9439 | | |
| 9440 | | > show #50.11 models |
| 9441 | | |
| 9442 | | > fitmap #50.11inMap #33.3 |
| 9443 | | |
| 9444 | | Missing or invalid "atomsOrMap" argument: only initial part "#50.11" of atom |
| 9445 | | specifier valid |
| 9446 | | |
| 9447 | | > fitmap #50.11 inMap #33.3 |
| 9448 | | |
| 9449 | | Fit molecule W20_C1_initial_model_no_wat_lig.pdb BA (#50.11) to map |
| 9450 | | W20_J160_run_ct6_body003.mrc (#33.3) using 1158 atoms |
| 9451 | | average map value = 0.0132, steps = 104 |
| 9452 | | shifted from previous position = 5.13 |
| 9453 | | rotated from previous position = 28.7 degrees |
| 9454 | | atoms outside contour = 711, contour level = 0.015167 |
| 9455 | | |
| 9456 | | Position of W20_C1_initial_model_no_wat_lig.pdb BA (#50.11) relative to |
| 9457 | | W20_J160_run_ct6_body003.mrc (#33.3) coordinates: |
| 9458 | | Matrix rotation and translation |
| 9459 | | -0.92321344 -0.13158780 -0.36105621 489.23124927 |
| 9460 | | 0.28319718 -0.86806041 -0.40776278 396.62096334 |
| 9461 | | -0.25976200 -0.47870218 0.83867033 84.55689237 |
| 9462 | | Axis -0.16389770 -0.23402916 0.95831513 |
| 9463 | | Axis point 230.41567989 235.72565549 0.00000000 |
| 9464 | | Rotation angle (degrees) 167.50152307 |
| 9465 | | Shift along axis -91.97259645 |
| 9466 | | |
| 9467 | | |
| 9468 | | > hide #50.11 models |
| 9469 | | |
| 9470 | | > show #39 models |
| 9471 | | |
| 9472 | | > fitmap #39 inMap #33.3 |
| 9473 | | |
| 9474 | | Fit molecule CopZ1_P61924.pdb (#39) to map W20_J160_run_ct6_body003.mrc |
| 9475 | | (#33.3) using 1420 atoms |
| 9476 | | average map value = 0.01141, steps = 92 |
| 9477 | | shifted from previous position = 7.91 |
| 9478 | | rotated from previous position = 10.3 degrees |
| 9479 | | atoms outside contour = 963, contour level = 0.015167 |
| 9480 | | |
| 9481 | | Position of CopZ1_P61924.pdb (#39) relative to W20_J160_run_ct6_body003.mrc |
| 9482 | | (#33.3) coordinates: |
| 9483 | | Matrix rotation and translation |
| 9484 | | -0.50106153 0.84740615 0.17561366 128.97282199 |
| 9485 | | -0.08865073 -0.25211715 0.96362752 130.68154277 |
| 9486 | | 0.86085911 0.46726840 0.20144938 92.25448210 |
| 9487 | | Axis -0.39337422 -0.54307030 -0.74184323 |
| 9488 | | Axis point 46.59255051 18.60088904 0.00000000 |
| 9489 | | Rotation angle (degrees) 140.88349075 |
| 9490 | | Shift along axis -190.14221075 |
| 9491 | | |
| 9492 | | |
| 9493 | | > select add #39 |
| 9494 | | |
| 9495 | | 1420 atoms, 1441 bonds, 177 residues, 1 model selected |
| 9496 | | |
| 9497 | | > view matrix models |
| 9498 | | > #39,-0.038392,0.25438,0.96634,124.66,0.50314,-0.8306,0.23864,135.53,0.86335,0.49537,-0.096103,99.479 |
| 9499 | | |
| 9500 | | > fitmap #39 inMap #33.3 |
| 9501 | | |
| 9502 | | Fit molecule CopZ1_P61924.pdb (#39) to map W20_J160_run_ct6_body003.mrc |
| 9503 | | (#33.3) using 1420 atoms |
| 9504 | | average map value = 0.0138, steps = 140 |
| 9505 | | shifted from previous position = 3.19 |
| 9506 | | rotated from previous position = 29.8 degrees |
| 9507 | | atoms outside contour = 768, contour level = 0.015167 |
| 9508 | | |
| 9509 | | Position of CopZ1_P61924.pdb (#39) relative to W20_J160_run_ct6_body003.mrc |
| 9510 | | (#33.3) coordinates: |
| 9511 | | Matrix rotation and translation |
| 9512 | | -0.59910220 0.35688559 0.71673513 128.54412804 |
| 9513 | | 0.33901457 -0.69789676 0.63087974 127.78779026 |
| 9514 | | 0.72535901 0.62094508 0.29712207 93.14122293 |
| 9515 | | Axis -0.44768773 -0.38862007 -0.80532611 |
| 9516 | | Axis point 38.57930905 41.25889004 0.00000000 |
| 9517 | | Rotation angle (degrees) 179.36426033 |
| 9518 | | Shift along axis -182.21758707 |
| 9519 | | |
| 9520 | | |
| 9521 | | > select subtract #39 |
| 9522 | | |
| 9523 | | Nothing selected |
| 9524 | | |
| 9525 | | > show #!34 models |
| 9526 | | |
| 9527 | | > hide #!34 models |
| 9528 | | |
| 9529 | | > show #!34 models |
| 9530 | | |
| 9531 | | > hide #!34 models |
| 9532 | | |
| 9533 | | > hide #39 models |
| 9534 | | |
| 9535 | | > hide #!50 models |
| 9536 | | |
| 9537 | | > show #!50 models |
| 9538 | | |
| 9539 | | > hide #!50 models |
| 9540 | | |
| 9541 | | > hide #50.1 models |
| 9542 | | |
| 9543 | | > show #!50 models |
| 9544 | | |
| 9545 | | > hide #!50 models |
| 9546 | | |
| 9547 | | > hide #52 models |
| 9548 | | |
| 9549 | | > show #!34 models |
| 9550 | | |
| 9551 | | > fitmap #33 inMap #33.3 |
| 9552 | | |
| 9553 | | Multiple maps for #33 |
| 9554 | | |
| 9555 | | > fitmap #34 inMap #33.2 |
| 9556 | | |
| 9557 | | Fit molecule CopA-F8WHL2.pdb (#34) to map W20_J160_run_ct6_body002.mrc (#33.2) |
| 9558 | | using 6696 atoms |
| 9559 | | average map value = 0.01229, steps = 144 |
| 9560 | | shifted from previous position = 0.768 |
| 9561 | | rotated from previous position = 0.619 degrees |
| 9562 | | atoms outside contour = 4035, contour level = 0.014809 |
| 9563 | | |
| 9564 | | Position of CopA-F8WHL2.pdb (#34) relative to W20_J160_run_ct6_body002.mrc |
| 9565 | | (#33.2) coordinates: |
| 9566 | | Matrix rotation and translation |
| 9567 | | -0.57247775 -0.57456895 -0.58492714 151.44699314 |
| 9568 | | 0.70447239 -0.70969024 0.00764404 125.42515832 |
| 9569 | | -0.41950911 -0.40768897 0.81104982 121.20707602 |
| 9570 | | Axis -0.30653667 -0.12208683 0.94399686 |
| 9571 | | Axis point 68.14146734 105.20078411 -0.00000000 |
| 9572 | | Rotation angle (degrees) 137.35447863 |
| 9573 | | Shift along axis 52.68228363 |
| 9574 | | |
| 9575 | | |
| 9576 | | > show #50.1 models |
| 9577 | | |
| 9578 | | > hide #50.1 models |
| 9579 | | |
| 9580 | | > show #50.1 models |
| 9581 | | |
| 9582 | | > hide #!50 models |
| 9583 | | |
| 9584 | | > hide #50.1 models |
| 9585 | | |
| 9586 | | > show #!50 models |
| 9587 | | |
| 9588 | | > show #50.1 models |
| 9589 | | |
| 9590 | | > hide #!50.3 models |
| 9591 | | |
| 9592 | | > show #!50.3 models |
| 9593 | | |
| 9594 | | > hide #!50.5 models |
| 9595 | | |
| 9596 | | > hide #!50.2 models |
| 9597 | | |
| 9598 | | > hide #!50.3 models |
| 9599 | | |
| 9600 | | > hide #50.6 models |
| 9601 | | |
| 9602 | | > hide #!50.8 models |
| 9603 | | |
| 9604 | | > hide #50.9 models |
| 9605 | | |
| 9606 | | > hide #!50.7 models |
| 9607 | | |
| 9608 | | > hide #!50.10 models |
| 9609 | | |
| 9610 | | > hide #50.1 models |
| 9611 | | |
| 9612 | | > show #!50.2 models |
| 9613 | | |
| 9614 | | > show #!50.3 models |
| 9615 | | |
| 9616 | | > fitmap #50.2 inMap #33.2 |
| 9617 | | |
| 9618 | | Fit molecule W20_C1_initial_model_no_wat_lig.pdb A1 (#50.2) to map |
| 9619 | | W20_J160_run_ct6_body002.mrc (#33.2) using 2510 atoms |
| 9620 | | average map value = 0.01804, steps = 44 |
| 9621 | | shifted from previous position = 0.476 |
| 9622 | | rotated from previous position = 0.223 degrees |
| 9623 | | atoms outside contour = 1048, contour level = 0.014809 |
| 9624 | | |
| 9625 | | Position of W20_C1_initial_model_no_wat_lig.pdb A1 (#50.2) relative to |
| 9626 | | W20_J160_run_ct6_body002.mrc (#33.2) coordinates: |
| 9627 | | Matrix rotation and translation |
| 9628 | | -0.95004228 0.18207545 -0.25351172 368.13646707 |
| 9629 | | 0.07472194 -0.65592625 -0.75111742 494.19899480 |
| 9630 | | -0.30304504 -0.73253620 0.60955264 227.59862474 |
| 9631 | | Axis 0.15525602 0.41387719 -0.89699567 |
| 9632 | | Axis point 211.61727753 293.61074195 0.00000000 |
| 9633 | | Rotation angle (degrees) 176.56933670 |
| 9634 | | Shift along axis 57.53811301 |
| 9635 | | |
| 9636 | | |
| 9637 | | > fitmap #50.3 inMap #33.2 |
| 9638 | | |
| 9639 | | Fit molecule W20_C1_initial_model_no_wat_lig.pdb A2 (#50.3) to map |
| 9640 | | W20_J160_run_ct6_body002.mrc (#33.2) using 3559 atoms |
| 9641 | | average map value = 0.01022, steps = 184 |
| 9642 | | shifted from previous position = 2.7 |
| 9643 | | rotated from previous position = 7.42 degrees |
| 9644 | | atoms outside contour = 2357, contour level = 0.014809 |
| 9645 | | |
| 9646 | | Position of W20_C1_initial_model_no_wat_lig.pdb A2 (#50.3) relative to |
| 9647 | | W20_J160_run_ct6_body002.mrc (#33.2) coordinates: |
| 9648 | | Matrix rotation and translation |
| 9649 | | -0.93274721 0.18386833 -0.31012106 379.03416947 |
| 9650 | | 0.07603460 -0.74050966 -0.66773063 496.69481023 |
| 9651 | | -0.35242216 -0.64640381 0.67672796 193.41619316 |
| 9652 | | Axis 0.18107201 0.35915085 -0.91554552 |
| 9653 | | Axis point 216.23338293 280.38752423 0.00000000 |
| 9654 | | Rotation angle (degrees) 176.62387365 |
| 9655 | | Shift along axis 69.93951212 |
| 9656 | | |
| 9657 | | |
| 9658 | | > hide #!33 models |
| 9659 | | |
| 9660 | | > hide #!34 models |
| 9661 | | |
| 9662 | | > show #!34 models |
| 9663 | | |
| 9664 | | > hide #!50.2 models |
| 9665 | | |
| 9666 | | > show #!50.2 models |
| 9667 | | |
| 9668 | | > hide #!50.2 models |
| 9669 | | |
| 9670 | | > ui mousemode right select |
| 9671 | | |
| 9672 | | Drag select of 223 residues |
| 9673 | | |
| 9674 | | > select up |
| 9675 | | |
| 9676 | | 2109 atoms, 2166 bonds, 262 residues, 1 model selected |
| 9677 | | |
| 9678 | | > select up |
| 9679 | | |
| 9680 | | 6696 atoms, 6852 bonds, 834 residues, 1 model selected |
| 9681 | | |
| 9682 | | > select down |
| 9683 | | |
| 9684 | | 2109 atoms, 2166 bonds, 262 residues, 1 model selected |
| 9685 | | |
| 9686 | | > select #34/A:127 |
| 9687 | | |
| 9688 | | 6 atoms, 5 bonds, 1 residue, 1 model selected |
| 9689 | | |
| 9690 | | > select clear |
| 9691 | | |
| 9692 | | Drag select of 1 residues |
| 9693 | | |
| 9694 | | > select up |
| 9695 | | |
| 9696 | | 6696 atoms, 6852 bonds, 834 residues, 1 model selected |
| 9697 | | |
| 9698 | | > select down |
| 9699 | | |
| 9700 | | 8 atoms, 1 residue, 1 model selected |
| 9701 | | Drag select of 190 residues |
| 9702 | | |
| 9703 | | > select up |
| 9704 | | |
| 9705 | | 1664 atoms, 1710 bonds, 206 residues, 1 model selected |
| 9706 | | |
| 9707 | | > select up |
| 9708 | | |
| 9709 | | 6696 atoms, 6852 bonds, 834 residues, 1 model selected |
| 9710 | | |
| 9711 | | > select down |
| 9712 | | |
| 9713 | | 1664 atoms, 1710 bonds, 206 residues, 1 model selected |
| 9714 | | |
| 9715 | | > delete sel |
| 9716 | | |
| 9717 | | Drag select of 63 residues, 2 pseudobonds |
| 9718 | | |
| 9719 | | > select up |
| 9720 | | |
| 9721 | | 788 atoms, 803 bonds, 2 pseudobonds, 96 residues, 2 models selected |
| 9722 | | |
| 9723 | | > delete sel |
| 9724 | | |
| 9725 | | Drag select of 19 residues, 2 pseudobonds |
| 9726 | | |
| 9727 | | > delete sel |
| 9728 | | |
| 9729 | | Drag select of 4 residues |
| 9730 | | |
| 9731 | | > delete sel |
| 9732 | | |
| 9733 | | > show #!50.2 models |
| 9734 | | |
| 9735 | | > show #!33 models |
| 9736 | | |
| 9737 | | > fitmap #34 inMap #33.2 |
| 9738 | | |
| 9739 | | Fit molecule CopA-F8WHL2.pdb (#34) to map W20_J160_run_ct6_body002.mrc (#33.2) |
| 9740 | | using 4046 atoms |
| 9741 | | average map value = 0.009675, steps = 164 |
| 9742 | | shifted from previous position = 0.697 |
| 9743 | | rotated from previous position = 2.08 degrees |
| 9744 | | atoms outside contour = 2782, contour level = 0.014809 |
| 9745 | | |
| 9746 | | Position of CopA-F8WHL2.pdb (#34) relative to W20_J160_run_ct6_body002.mrc |
| 9747 | | (#33.2) coordinates: |
| 9748 | | Matrix rotation and translation |
| 9749 | | -0.58668811 -0.55339986 -0.59122387 151.25489618 |
| 9750 | | 0.67908972 -0.73393831 0.01310416 124.24263215 |
| 9751 | | -0.44117369 -0.39380599 0.80640103 120.81607944 |
| 9752 | | Axis -0.31143421 -0.11484294 0.94330262 |
| 9753 | | Axis point 69.89652487 102.51139715 0.00000000 |
| 9754 | | Rotation angle (degrees) 139.21031634 |
| 9755 | | Shift along axis 52.59178621 |
| 9756 | | |
| 9757 | | |
| 9758 | | > fitmap #50.3 inMap #33.2 |
| 9759 | | |
| 9760 | | Fit molecule W20_C1_initial_model_no_wat_lig.pdb A2 (#50.3) to map |
| 9761 | | W20_J160_run_ct6_body002.mrc (#33.2) using 3559 atoms |
| 9762 | | average map value = 0.01022, steps = 160 |
| 9763 | | shifted from previous position = 0.0132 |
| 9764 | | rotated from previous position = 0.015 degrees |
| 9765 | | atoms outside contour = 2353, contour level = 0.014809 |
| 9766 | | |
| 9767 | | Position of W20_C1_initial_model_no_wat_lig.pdb A2 (#50.3) relative to |
| 9768 | | W20_J160_run_ct6_body002.mrc (#33.2) coordinates: |
| 9769 | | Matrix rotation and translation |
| 9770 | | -0.93280742 0.18368258 -0.31005003 379.08438259 |
| 9771 | | 0.07605879 -0.74062741 -0.66759726 496.69733667 |
| 9772 | | -0.35225754 -0.64632171 0.67689207 193.32199255 |
| 9773 | | Axis 0.18099805 0.35907301 -0.91559067 |
| 9774 | | Axis point 216.22488098 280.37147530 0.00000000 |
| 9775 | | Rotation angle (degrees) 176.63062081 |
| 9776 | | Shift along axis 69.96032790 |
| 9777 | | |
| 9778 | | |
| 9779 | | > combine #34 |
| 9780 | | |
| 9781 | | > combine #50.3 |
| 9782 | | |
| 9783 | | > hide #!50.3 models |
| 9784 | | |
| 9785 | | > hide #!34 models |
| 9786 | | |
| 9787 | | > hide #!33.4 models |
| 9788 | | |
| 9789 | | > show #!33.4 models |
| 9790 | | |
| 9791 | | > hide #!33 models |
| 9792 | | |
| 9793 | | > hide #!58 models |
| 9794 | | |
| 9795 | | > select clear |
| 9796 | | |
| 9797 | | > hide #!50.2 models |
| 9798 | | |
| 9799 | | Drag select of 243 residues |
| 9800 | | |
| 9801 | | > select up |
| 9802 | | |
| 9803 | | 2056 atoms, 2095 bonds, 259 residues, 1 model selected |
| 9804 | | |
| 9805 | | > select up |
| 9806 | | |
| 9807 | | 4046 atoms, 4125 bonds, 509 residues, 1 model selected |
| 9808 | | |
| 9809 | | > select up |
| 9810 | | |
| 9811 | | 101979 atoms, 103831 bonds, 12855 residues, 91 models selected |
| 9812 | | |
| 9813 | | > select clear |
| 9814 | | |
| 9815 | | Drag select of 274 residues |
| 9816 | | |
| 9817 | | > select up |
| 9818 | | |
| 9819 | | 2192 atoms, 2235 bonds, 276 residues, 1 model selected |
| 9820 | | |
| 9821 | | > delete sel |
| 9822 | | |
| 9823 | | Drag select of 2 residues, 1 pseudobonds |
| 9824 | | |
| 9825 | | > delete sel |
| 9826 | | |
| 9827 | | > show #!33 models |
| 9828 | | |
| 9829 | | > fitmap #57 inMap #33.1 |
| 9830 | | |
| 9831 | | Fit molecule copy of CopA-F8WHL2.pdb (#57) to map W20_J160_run_ct6_body001.mrc |
| 9832 | | (#33.1) using 1842 atoms |
| 9833 | | average map value = 0.01399, steps = 76 |
| 9834 | | shifted from previous position = 7.58 |
| 9835 | | rotated from previous position = 11.9 degrees |
| 9836 | | atoms outside contour = 1005, contour level = 0.014811 |
| 9837 | | |
| 9838 | | Position of copy of CopA-F8WHL2.pdb (#57) relative to |
| 9839 | | W20_J160_run_ct6_body001.mrc (#33.1) coordinates: |
| 9840 | | Matrix rotation and translation |
| 9841 | | -0.66070189 -0.61639558 -0.42840344 148.88789464 |
| 9842 | | 0.70166098 -0.70992235 -0.06068050 124.32932168 |
| 9843 | | -0.26672999 -0.34068570 0.90154776 127.22528478 |
| 9844 | | Axis -0.20632088 -0.11912853 0.97120548 |
| 9845 | | Axis point 61.04505270 103.19655495 0.00000000 |
| 9846 | | Rotation angle (degrees) 137.26821223 |
| 9847 | | Shift along axis 78.03204323 |
| 9848 | | |
| 9849 | | |
| 9850 | | > hide #!57 models |
| 9851 | | |
| 9852 | | > hide #!33.4 models |
| 9853 | | |
| 9854 | | > show #!33.4 models |
| 9855 | | |
| 9856 | | > hide #!33 models |
| 9857 | | |
| 9858 | | > show #!58 models |
| 9859 | | |
| 9860 | | Drag select of 104 residues, 1 pseudobonds |
| 9861 | | |
| 9862 | | > select clear |
| 9863 | | |
| 9864 | | Drag select of 282 residues, 6 pseudobonds |
| 9865 | | |
| 9866 | | > delete sel |
| 9867 | | |
| 9868 | | > fitmap #87 inMap #33.1 |
| 9869 | | |
| 9870 | | No atoms or maps for #87 |
| 9871 | | |
| 9872 | | > fitmap #58 inMap #33.1 |
| 9873 | | |
| 9874 | | Fit molecule copy of W20_C1_initial_model_no_wat_lig.pdb A2 (#58) to map |
| 9875 | | W20_J160_run_ct6_body001.mrc (#33.1) using 1297 atoms |
| 9876 | | average map value = 0.01501, steps = 108 |
| 9877 | | shifted from previous position = 10.8 |
| 9878 | | rotated from previous position = 12.4 degrees |
| 9879 | | atoms outside contour = 664, contour level = 0.014811 |
| 9880 | | |
| 9881 | | Position of copy of W20_C1_initial_model_no_wat_lig.pdb A2 (#58) relative to |
| 9882 | | W20_J160_run_ct6_body001.mrc (#33.1) coordinates: |
| 9883 | | Matrix rotation and translation |
| 9884 | | -0.95774575 0.09731996 -0.27065088 396.29244001 |
| 9885 | | 0.15737130 -0.61033953 -0.77635039 474.71597461 |
| 9886 | | -0.24074332 -0.78613897 0.56923472 242.75449330 |
| 9887 | | Axis -0.14438058 -0.44113353 0.88575135 |
| 9888 | | Axis point 213.60576536 301.52927607 0.00000000 |
| 9889 | | Rotation angle (degrees) 178.05738481 |
| 9890 | | Shift along axis -51.60994703 |
| 9891 | | |
| 9892 | | |
| 9893 | | > show #!33 models |
| 9894 | | |
| 9895 | | > show #!57 models |
| 9896 | | |
| 9897 | | > hide #!58 models |
| 9898 | | |
| 9899 | | > show #!58 models |
| 9900 | | |
| 9901 | | > hide #!57 models |
| 9902 | | |
| 9903 | | > show #!57 models |
| 9904 | | |
| 9905 | | > hide #!33 models |
| 9906 | | |
| 9907 | | > show #!34 models |
| 9908 | | |
| 9909 | | > hide #!57 models |
| 9910 | | |
| 9911 | | > hide #!58 models |
| 9912 | | |
| 9913 | | > show #!58 models |
| 9914 | | |
| 9915 | | > hide #!58 models |
| 9916 | | |
| 9917 | | > show #!58 models |
| 9918 | | |
| 9919 | | > hide #!58 models |
| 9920 | | |
| 9921 | | Drag select of 144 residues |
| 9922 | | |
| 9923 | | > select clear |
| 9924 | | |
| 9925 | | [Repeated 1 time(s)] |
| 9926 | | |
| 9927 | | > combine #34 |
| 9928 | | |
| 9929 | | > hide #!34 models |
| 9930 | | |
| 9931 | | > show #!58 models |
| 9932 | | |
| 9933 | | > hide #!58 models |
| 9934 | | |
| 9935 | | > show #!58 models |
| 9936 | | |
| 9937 | | > hide #!58 models |
| 9938 | | |
| 9939 | | > show #!58 models |
| 9940 | | |
| 9941 | | > hide #!58 models |
| 9942 | | |
| 9943 | | > show #!58 models |
| 9944 | | |
| 9945 | | > hide #!58 models |
| 9946 | | |
| 9947 | | Drag select of 161 residues |
| 9948 | | |
| 9949 | | > select up |
| 9950 | | |
| 9951 | | 1359 atoms, 1382 bonds, 172 residues, 1 model selected |
| 9952 | | |
| 9953 | | > show #!58 models |
| 9954 | | |
| 9955 | | > hide #!58 models |
| 9956 | | |
| 9957 | | > delete sel |
| 9958 | | |
| 9959 | | Drag select of 9 residues |
| 9960 | | |
| 9961 | | > delete sel |
| 9962 | | |
| 9963 | | > fitmap #59 inMap #33.1 |
| 9964 | | |
| 9965 | | Fit molecule copy of CopA-F8WHL2.pdb (#59) to map W20_J160_run_ct6_body001.mrc |
| 9966 | | (#33.1) using 2618 atoms |
| 9967 | | average map value = 0.00517, steps = 108 |
| 9968 | | shifted from previous position = 1.61 |
| 9969 | | rotated from previous position = 4.74 degrees |
| 9970 | | atoms outside contour = 2287, contour level = 0.014811 |
| 9971 | | |
| 9972 | | Position of copy of CopA-F8WHL2.pdb (#59) relative to |
| 9973 | | W20_J160_run_ct6_body001.mrc (#33.1) coordinates: |
| 9974 | | Matrix rotation and translation |
| 9975 | | -0.61097397 -0.53341027 -0.58496522 151.41100776 |
| 9976 | | 0.68780030 -0.72352924 -0.05861913 123.94831007 |
| 9977 | | -0.39197139 -0.43815401 0.80893726 122.28836749 |
| 9978 | | Axis -0.29346029 -0.14922482 0.94425262 |
| 9979 | | Axis point 69.10859861 104.04335802 0.00000000 |
| 9980 | | Rotation angle (degrees) 139.71015631 |
| 9981 | | Shift along axis 52.54182884 |
| 9982 | | |
| 9983 | | |
| 9984 | | > fitmap #59 inMap #33.2 |
| 9985 | | |
| 9986 | | Fit molecule copy of CopA-F8WHL2.pdb (#59) to map W20_J160_run_ct6_body002.mrc |
| 9987 | | (#33.2) using 2618 atoms |
| 9988 | | average map value = 0.0148, steps = 72 |
| 9989 | | shifted from previous position = 1.57 |
| 9990 | | rotated from previous position = 4.66 degrees |
| 9991 | | atoms outside contour = 1354, contour level = 0.014809 |
| 9992 | | |
| 9993 | | Position of copy of CopA-F8WHL2.pdb (#59) relative to |
| 9994 | | W20_J160_run_ct6_body002.mrc (#33.2) coordinates: |
| 9995 | | Matrix rotation and translation |
| 9996 | | -0.58463217 -0.55176650 -0.59477640 151.19300478 |
| 9997 | | 0.68006639 -0.73305905 0.01158206 124.19731133 |
| 9998 | | -0.44239681 -0.39771619 0.80380775 120.82904766 |
| 9999 | | Axis -0.31316702 -0.11659043 0.94251424 |
| 10000 | | Axis point 69.96688260 102.62111999 0.00000000 |
| 10001 | | Rotation angle (degrees) 139.19532441 |
| 10002 | | Shift along axis 52.05421740 |
| 10003 | | |
| 10004 | | |
| 10005 | | > show #!33 models |
| 10006 | | |
| 10007 | | > show #!58 models |
| 10008 | | |
| 10009 | | > show #!50.2 models |
| 10010 | | |
| 10011 | | > hide #!33 models |
| 10012 | | |
| 10013 | | > show #!34 models |
| 10014 | | |
| 10015 | | > hide #!34 models |
| 10016 | | |
| 10017 | | > show #!34 models |
| 10018 | | |
| 10019 | | > hide #!34 models |
| 10020 | | |
| 10021 | | > show #!34 models |
| 10022 | | |
| 10023 | | > hide #!34 models |
| 10024 | | |
| 10025 | | > show #!34 models |
| 10026 | | |
| 10027 | | > hide #!50.2 models |
| 10028 | | |
| 10029 | | > show #!50.2 models |
| 10030 | | |
| 10031 | | > hide #!34 models |
| 10032 | | |
| 10033 | | > show #!34 models |
| 10034 | | |
| 10035 | | > hide #!34 models |
| 10036 | | |
| 10037 | | > show #!34 models |
| 10038 | | |
| 10039 | | > hide #!34 models |
| 10040 | | |
| 10041 | | > hide #!59 models |
| 10042 | | |
| 10043 | | > show #!59 models |
| 10044 | | |
| 10045 | | > show #!57 models |
| 10046 | | |
| 10047 | | > hide #!57 models |
| 10048 | | |
| 10049 | | > show #!57 models |
| 10050 | | |
| 10051 | | > hide #!57 models |
| 10052 | | |
| 10053 | | > hide #!59 models |
| 10054 | | |
| 10055 | | > show #!57 models |
| 10056 | | |
| 10057 | | > hide #!58 models |
| 10058 | | |
| 10059 | | > hide #!57 models |
| 10060 | | |
| 10061 | | > show #!33 models |
| 10062 | | |
| 10063 | | > show #!57 models |
| 10064 | | |
| 10065 | | > show #!58 models |
| 10066 | | |
| 10067 | | > hide #!58 models |
| 10068 | | |
| 10069 | | > show #!59 models |
| 10070 | | |
| 10071 | | > hide #!59 models |
| 10072 | | |
| 10073 | | > show #!59 models |
| 10074 | | |
| 10075 | | > hide #!57 models |
| 10076 | | |
| 10077 | | > show #!58 models |
| 10078 | | |
| 10079 | | > hide #!33 models |
| 10080 | | |
| 10081 | | > show #51 models |
| 10082 | | |
| 10083 | | > combine #51 |
| 10084 | | |
| 10085 | | > hide #51 models |
| 10086 | | |
| 10087 | | > hide #!59 models |
| 10088 | | |
| 10089 | | > hide #!58 models |
| 10090 | | |
| 10091 | | > hide #!50.2 models |
| 10092 | | |
| 10093 | | Drag select of 667 residues |
| 10094 | | |
| 10095 | | > select up |
| 10096 | | |
| 10097 | | 6183 atoms, 6300 bonds, 784 residues, 1 model selected |
| 10098 | | |
| 10099 | | > delete sel |
| 10100 | | |
| 10101 | | Drag select of 87 residues, 10 pseudobonds |
| 10102 | | |
| 10103 | | > select clear |
| 10104 | | |
| 10105 | | Drag select of 61 residues, 5 pseudobonds |
| 10106 | | |
| 10107 | | > select up |
| 10108 | | |
| 10109 | | 758 atoms, 752 bonds, 5 pseudobonds, 95 residues, 2 models selected |
| 10110 | | |
| 10111 | | > delete sel |
| 10112 | | |
| 10113 | | Drag select of 27 residues, 3 pseudobonds |
| 10114 | | |
| 10115 | | > select up |
| 10116 | | |
| 10117 | | 2800 atoms, 2874 bonds, 3 pseudobonds, 345 residues, 2 models selected |
| 10118 | | |
| 10119 | | > select down |
| 10120 | | |
| 10121 | | 210 atoms, 3 pseudobonds, 27 residues, 2 models selected |
| 10122 | | |
| 10123 | | > delete sel |
| 10124 | | |
| 10125 | | > fitmap #60 inMap #33.2 |
| 10126 | | |
| 10127 | | Fit molecule copy of A_fixed_model_2_A.pdb (#60) to map |
| 10128 | | W20_J160_run_ct6_body002.mrc (#33.2) using 2590 atoms |
| 10129 | | average map value = 0.01604, steps = 212 |
| 10130 | | shifted from previous position = 8.36 |
| 10131 | | rotated from previous position = 37.7 degrees |
| 10132 | | atoms outside contour = 1297, contour level = 0.014809 |
| 10133 | | |
| 10134 | | Position of copy of A_fixed_model_2_A.pdb (#60) relative to |
| 10135 | | W20_J160_run_ct6_body002.mrc (#33.2) coordinates: |
| 10136 | | Matrix rotation and translation |
| 10137 | | -0.12666898 -0.97960527 -0.15597590 240.27472629 |
| 10138 | | 0.03811656 -0.16193319 0.98606530 35.82542257 |
| 10139 | | -0.99121244 0.11895862 0.05785109 200.76320490 |
| 10140 | | Axis -0.55003121 0.52981504 0.64557082 |
| 10141 | | Axis point 195.41347917 29.75172699 0.00000000 |
| 10142 | | Rotation angle (degrees) 127.97921254 |
| 10143 | | Shift along axis 16.42911600 |
| 10144 | | |
| 10145 | | |
| 10146 | | > show #!33 models |
| 10147 | | |
| 10148 | | > show #50.1 models |
| 10149 | | |
| 10150 | | > hide #50.1 models |
| 10151 | | |
| 10152 | | > show #!50.2 models |
| 10153 | | |
| 10154 | | > select add #60 |
| 10155 | | |
| 10156 | | 2590 atoms, 2665 bonds, 318 residues, 1 model selected |
| 10157 | | |
| 10158 | | > ui mousemode right "rotate selected models" |
| 10159 | | |
| 10160 | | > view matrix models |
| 10161 | | > #60,-0.3575,-0.93077,-0.076565,262.87,-0.90319,0.36543,-0.22518,271.82,0.23757,-0.011349,-0.9713,201.17 |
| 10162 | | |
| 10163 | | > ui tool show Matchmaker |
| 10164 | | |
| 10165 | | > matchmaker #!60 to #50.2 |
| 10166 | | |
| 10167 | | Computing secondary structure |
| 10168 | | Parameters |
| 10169 | | --- |
| 10170 | | Chain pairing | bb |
| 10171 | | Alignment algorithm | Needleman-Wunsch |
| 10172 | | Similarity matrix | BLOSUM-62 |
| 10173 | | SS fraction | 0.3 |
| 10174 | | Gap open (HH/SS/other) | 18/18/6 |
| 10175 | | Gap extend | 1 |
| 10176 | | SS matrix | | | H | S | O |
| 10177 | | ---|---|---|--- |
| 10178 | | H | 6 | -9 | -6 |
| 10179 | | S | | 6 | -6 |
| 10180 | | O | | | 4 |
| 10181 | | Iteration cutoff | 2 |
| 10182 | | |
| 10183 | | Matchmaker W20_C1_initial_model_no_wat_lig.pdb A1, chain A1 (#50.2) with copy |
| 10184 | | of A_fixed_model_2_A.pdb, chain A (#60), sequence alignment score = 1588.3 |
| 10185 | | RMSD between 296 pruned atom pairs is 0.254 angstroms; (across all 296 pairs: |
| 10186 | | 0.254) |
| 10187 | | |
| 10188 | | |
| 10189 | | > hide #!50.2 models |
| 10190 | | |
| 10191 | | > hide #!50 models |
| 10192 | | |
| 10193 | | > show #!50 models |
| 10194 | | |
| 10195 | | > hide #!50 models |
| 10196 | | |
| 10197 | | > select subtract #60 |
| 10198 | | |
| 10199 | | Nothing selected |
| 10200 | | |
| 10201 | | > show #!50.2 models |
| 10202 | | |
| 10203 | | > show #51 models |
| 10204 | | |
| 10205 | | > hide #51 models |
| 10206 | | |
| 10207 | | > show #51 models |
| 10208 | | |
| 10209 | | > hide #51 models |
| 10210 | | |
| 10211 | | > combine #51 |
| 10212 | | |
| 10213 | | > hide #!33 models |
| 10214 | | |
| 10215 | | > hide #!50.2 models |
| 10216 | | |
| 10217 | | > hide #!60 models |
| 10218 | | |
| 10219 | | > ui mousemode right select |
| 10220 | | |
| 10221 | | Drag select of 103 residues |
| 10222 | | |
| 10223 | | > select up |
| 10224 | | |
| 10225 | | 1168 atoms, 1193 bonds, 145 residues, 1 model selected |
| 10226 | | |
| 10227 | | > delete sel |
| 10228 | | |
| 10229 | | > hide #!61 models |
| 10230 | | |
| 10231 | | > show #!60 models |
| 10232 | | |
| 10233 | | > hide #!60 models |
| 10234 | | |
| 10235 | | > show #!61 models |
| 10236 | | |
| 10237 | | > select #61/A:318 |
| 10238 | | |
| 10239 | | 9 atoms, 8 bonds, 1 residue, 1 model selected |
| 10240 | | |
| 10241 | | > select up |
| 10242 | | |
| 10243 | | 47 atoms, 47 bonds, 6 residues, 1 model selected |
| 10244 | | |
| 10245 | | > select up |
| 10246 | | |
| 10247 | | 7736 atoms, 7896 bonds, 978 residues, 1 model selected |
| 10248 | | |
| 10249 | | > select down |
| 10250 | | |
| 10251 | | 47 atoms, 47 bonds, 6 residues, 1 model selected |
| 10252 | | |
| 10253 | | > delete sel |
| 10254 | | |
| 10255 | | Drag select of 100 residues, 3 pseudobonds |
| 10256 | | |
| 10257 | | > select up |
| 10258 | | |
| 10259 | | 1091 atoms, 1121 bonds, 3 pseudobonds, 134 residues, 2 models selected |
| 10260 | | |
| 10261 | | > select down |
| 10262 | | |
| 10263 | | 793 atoms, 3 pseudobonds, 100 residues, 2 models selected |
| 10264 | | |
| 10265 | | > select clear |
| 10266 | | |
| 10267 | | > select #61/A:1 |
| 10268 | | |
| 10269 | | 8 atoms, 7 bonds, 1 residue, 1 model selected |
| 10270 | | |
| 10271 | | > select up |
| 10272 | | |
| 10273 | | 533 atoms, 549 bonds, 64 residues, 1 model selected |
| 10274 | | |
| 10275 | | > delete sel |
| 10276 | | |
| 10277 | | Drag select of 9 residues, 1 pseudobonds |
| 10278 | | |
| 10279 | | > delete sel |
| 10280 | | |
| 10281 | | Drag select of 26 residues, 1 pseudobonds |
| 10282 | | |
| 10283 | | > select up |
| 10284 | | |
| 10285 | | 258 atoms, 264 bonds, 1 pseudobond, 33 residues, 2 models selected |
| 10286 | | |
| 10287 | | > delete sel |
| 10288 | | |
| 10289 | | Drag select of 7 residues |
| 10290 | | |
| 10291 | | > select clear |
| 10292 | | |
| 10293 | | [Repeated 1 time(s)]Drag select of 13 residues |
| 10294 | | Drag select of 48 residues |
| 10295 | | |
| 10296 | | > select up |
| 10297 | | |
| 10298 | | 507 atoms, 517 bonds, 62 residues, 1 model selected |
| 10299 | | |
| 10300 | | > select down |
| 10301 | | |
| 10302 | | 384 atoms, 48 residues, 1 model selected |
| 10303 | | |
| 10304 | | > select clear |
| 10305 | | |
| 10306 | | Drag select of 50 residues |
| 10307 | | |
| 10308 | | > select up |
| 10309 | | |
| 10310 | | 507 atoms, 517 bonds, 62 residues, 1 model selected |
| 10311 | | |
| 10312 | | > select clear |
| 10313 | | |
| 10314 | | Drag select of 1 residues |
| 10315 | | |
| 10316 | | > select up |
| 10317 | | |
| 10318 | | 42 atoms, 42 bonds, 6 residues, 1 model selected |
| 10319 | | |
| 10320 | | > delete sel |
| 10321 | | |
| 10322 | | > select #61/A:263 |
| 10323 | | |
| 10324 | | 8 atoms, 7 bonds, 1 residue, 1 model selected |
| 10325 | | |
| 10326 | | > select up |
| 10327 | | |
| 10328 | | 44 atoms, 43 bonds, 6 residues, 1 model selected |
| 10329 | | |
| 10330 | | > delete sel |
| 10331 | | |
| 10332 | | > select clear |
| 10333 | | |
| 10334 | | > select #61/A:258 |
| 10335 | | |
| 10336 | | 10 atoms, 10 bonds, 1 residue, 1 model selected |
| 10337 | | |
| 10338 | | > select clear |
| 10339 | | |
| 10340 | | Drag select of 4 residues |
| 10341 | | |
| 10342 | | > select clear |
| 10343 | | |
| 10344 | | Drag select of 4 residues |
| 10345 | | |
| 10346 | | > select clear |
| 10347 | | |
| 10348 | | Drag select of 5 residues |
| 10349 | | |
| 10350 | | > select up |
| 10351 | | |
| 10352 | | 121 atoms, 122 bonds, 14 residues, 1 model selected |
| 10353 | | |
| 10354 | | > delete sel |
| 10355 | | |
| 10356 | | > select clear |
| 10357 | | |
| 10358 | | > select #61/A:273 |
| 10359 | | |
| 10360 | | 8 atoms, 7 bonds, 1 residue, 1 model selected |
| 10361 | | |
| 10362 | | > select up |
| 10363 | | |
| 10364 | | 54 atoms, 55 bonds, 6 residues, 1 model selected |
| 10365 | | |
| 10366 | | > delete sel |
| 10367 | | |
| 10368 | | > select #61/A:278 |
| 10369 | | |
| 10370 | | 8 atoms, 7 bonds, 1 residue, 1 model selected |
| 10371 | | |
| 10372 | | > select clear |
| 10373 | | |
| 10374 | | Drag select of 1 residues |
| 10375 | | Drag select of 6 residues |
| 10376 | | |
| 10377 | | > select up |
| 10378 | | |
| 10379 | | 90 atoms, 90 bonds, 11 residues, 1 model selected |
| 10380 | | |
| 10381 | | > fitmap #60 inMap #33.2 |
| 10382 | | |
| 10383 | | Fit molecule copy of A_fixed_model_2_A.pdb (#60) to map |
| 10384 | | W20_J160_run_ct6_body002.mrc (#33.2) using 2590 atoms |
| 10385 | | average map value = 0.01743, steps = 48 |
| 10386 | | shifted from previous position = 0.0339 |
| 10387 | | rotated from previous position = 0.278 degrees |
| 10388 | | atoms outside contour = 1142, contour level = 0.014809 |
| 10389 | | |
| 10390 | | Position of copy of A_fixed_model_2_A.pdb (#60) relative to |
| 10391 | | W20_J160_run_ct6_body002.mrc (#33.2) coordinates: |
| 10392 | | Matrix rotation and translation |
| 10393 | | -0.44176945 -0.87418787 -0.20158210 278.02704219 |
| 10394 | | -0.65633376 0.16174616 0.73692889 133.70992844 |
| 10395 | | -0.61160916 0.45785781 -0.64521352 224.98469771 |
| 10396 | | Axis -0.51515506 0.75689501 0.40215074 |
| 10397 | | Axis point 203.25110647 0.00000000 49.34812669 |
| 10398 | | Rotation angle (degrees) 164.28447736 |
| 10399 | | Shift along axis 48.45510328 |
| 10400 | | |
| 10401 | | |
| 10402 | | > delete sel |
| 10403 | | |
| 10404 | | Drag select of 16 residues, 1 pseudobonds |
| 10405 | | |
| 10406 | | > select up |
| 10407 | | |
| 10408 | | 205 atoms, 209 bonds, 1 pseudobond, 25 residues, 2 models selected |
| 10409 | | |
| 10410 | | > select down |
| 10411 | | |
| 10412 | | 126 atoms, 1 pseudobond, 16 residues, 2 models selected |
| 10413 | | |
| 10414 | | > delete sel |
| 10415 | | |
| 10416 | | Drag select of 2 residues |
| 10417 | | |
| 10418 | | > delete sel |
| 10419 | | |
| 10420 | | > show #!60 models |
| 10421 | | |
| 10422 | | > show #!33 models |
| 10423 | | |
| 10424 | | > fitmap #61 inMap #33.1 |
| 10425 | | |
| 10426 | | Fit molecule copy of A_fixed_model_2_A.pdb (#61) to map |
| 10427 | | W20_J160_run_ct6_body001.mrc (#33.1) using 7151 atoms |
| 10428 | | average map value = 0.004441, steps = 228 |
| 10429 | | shifted from previous position = 5.35 |
| 10430 | | rotated from previous position = 8.58 degrees |
| 10431 | | atoms outside contour = 6297, contour level = 0.014811 |
| 10432 | | |
| 10433 | | Position of copy of A_fixed_model_2_A.pdb (#61) relative to |
| 10434 | | W20_J160_run_ct6_body001.mrc (#33.1) coordinates: |
| 10435 | | Matrix rotation and translation |
| 10436 | | -0.45222649 -0.82892764 -0.32919625 288.15586650 |
| 10437 | | -0.57376739 -0.01220253 0.81892739 121.44819917 |
| 10438 | | -0.68284858 0.55922273 -0.47009335 215.98047971 |
| 10439 | | Axis -0.51166679 0.69676128 0.50271344 |
| 10440 | | Axis point 206.53531672 0.00000000 35.33484489 |
| 10441 | | Rotation angle (degrees) 165.29851626 |
| 10442 | | Shift along axis 45.75690645 |
| 10443 | | |
| 10444 | | |
| 10445 | | > fitmap #61 inMap #33.2 |
| 10446 | | |
| 10447 | | Fit molecule copy of A_fixed_model_2_A.pdb (#61) to map |
| 10448 | | W20_J160_run_ct6_body002.mrc (#33.2) using 7151 atoms |
| 10449 | | average map value = 0.005548, steps = 2000 |
| 10450 | | shifted from previous position = 4.46 |
| 10451 | | rotated from previous position = 9.55 degrees |
| 10452 | | atoms outside contour = 5855, contour level = 0.014809 |
| 10453 | | |
| 10454 | | Position of copy of A_fixed_model_2_A.pdb (#61) relative to |
| 10455 | | W20_J160_run_ct6_body002.mrc (#33.2) coordinates: |
| 10456 | | Matrix rotation and translation |
| 10457 | | -0.32348189 -0.90777241 -0.26703693 271.47554220 |
| 10458 | | -0.61054188 -0.01536719 0.79183487 128.17226278 |
| 10459 | | -0.72290946 0.41918147 -0.54926207 231.58959567 |
| 10460 | | Axis -0.56499205 0.69116330 0.45064096 |
| 10461 | | Axis point 214.35725794 0.00000000 37.95145878 |
| 10462 | | Rotation angle (degrees) 160.74418801 |
| 10463 | | Shift along axis 39.57019701 |
| 10464 | | |
| 10465 | | |
| 10466 | | > combine #61 |
| 10467 | | |
| 10468 | | > hide #!61 models |
| 10469 | | |
| 10470 | | > hide #!60 models |
| 10471 | | |
| 10472 | | > hide #!50 models |
| 10473 | | |
| 10474 | | > show #!50 models |
| 10475 | | |
| 10476 | | > hide #!33 models |
| 10477 | | |
| 10478 | | Drag select of 1 residues |
| 10479 | | |
| 10480 | | > select up |
| 10481 | | |
| 10482 | | 7151 atoms, 7294 bonds, 906 residues, 1 model selected |
| 10483 | | |
| 10484 | | > select clear |
| 10485 | | |
| 10486 | | Drag select of 169 residues |
| 10487 | | |
| 10488 | | > select up |
| 10489 | | |
| 10490 | | 1541 atoms, 1568 bonds, 193 residues, 1 model selected |
| 10491 | | |
| 10492 | | > delete sel |
| 10493 | | |
| 10494 | | Drag select of 51 residues, 1 pseudobonds |
| 10495 | | |
| 10496 | | > select up |
| 10497 | | |
| 10498 | | 646 atoms, 657 bonds, 1 pseudobond, 82 residues, 2 models selected |
| 10499 | | |
| 10500 | | > delete sel |
| 10501 | | |
| 10502 | | Drag select of 4 residues |
| 10503 | | |
| 10504 | | > select clear |
| 10505 | | |
| 10506 | | Drag select of 3 residues |
| 10507 | | |
| 10508 | | > select clear |
| 10509 | | |
| 10510 | | [Repeated 2 time(s)]Drag select of 20 residues, 2 pseudobonds |
| 10511 | | |
| 10512 | | > select up |
| 10513 | | |
| 10514 | | 796 atoms, 812 bonds, 2 pseudobonds, 108 residues, 2 models selected |
| 10515 | | |
| 10516 | | > delete sel |
| 10517 | | |
| 10518 | | Drag select of 8 residues, 1 pseudobonds |
| 10519 | | |
| 10520 | | > select clear |
| 10521 | | |
| 10522 | | Drag select of 15 residues |
| 10523 | | |
| 10524 | | > delete sel |
| 10525 | | |
| 10526 | | > select clear |
| 10527 | | |
| 10528 | | > delete sel |
| 10529 | | |
| 10530 | | > select clear |
| 10531 | | |
| 10532 | | > delete sel |
| 10533 | | |
| 10534 | | > close #62 |
| 10535 | | |
| 10536 | | > combine #61 |
| 10537 | | |
| 10538 | | Drag select of 269 residues |
| 10539 | | |
| 10540 | | > select up |
| 10541 | | |
| 10542 | | 2282 atoms, 2326 bonds, 286 residues, 1 model selected |
| 10543 | | |
| 10544 | | > delete sel |
| 10545 | | |
| 10546 | | Drag select of 37 residues, 2 pseudobonds |
| 10547 | | |
| 10548 | | > select up |
| 10549 | | |
| 10550 | | 738 atoms, 753 bonds, 2 pseudobonds, 101 residues, 2 models selected |
| 10551 | | |
| 10552 | | > delete sel |
| 10553 | | |
| 10554 | | Drag select of 10 residues |
| 10555 | | |
| 10556 | | > delete sel |
| 10557 | | |
| 10558 | | > close #62 |
| 10559 | | |
| 10560 | | > combine #61 |
| 10561 | | |
| 10562 | | > select clear |
| 10563 | | |
| 10564 | | [Repeated 1 time(s)] |
| 10565 | | |
| 10566 | | > select #62/A:873 |
| 10567 | | |
| 10568 | | 4 atoms, 3 bonds, 1 residue, 1 model selected |
| 10569 | | |
| 10570 | | > select clear |
| 10571 | | |
| 10572 | | Drag select of 1 residues |
| 10573 | | |
| 10574 | | > select up |
| 10575 | | |
| 10576 | | 645 atoms, 657 bonds, 90 residues, 1 model selected |
| 10577 | | |
| 10578 | | > delete sel |
| 10579 | | |
| 10580 | | Drag select of 309 residues |
| 10581 | | |
| 10582 | | > delete sel |
| 10583 | | |
| 10584 | | > fitmap #62 inMap #33.1 |
| 10585 | | |
| 10586 | | Fit molecule copy of copy of A_fixed_model_2_A.pdb (#62) to map |
| 10587 | | W20_J160_run_ct6_body001.mrc (#33.1) using 4038 atoms |
| 10588 | | average map value = 0.007876, steps = 128 |
| 10589 | | shifted from previous position = 3.44 |
| 10590 | | rotated from previous position = 10.8 degrees |
| 10591 | | atoms outside contour = 3167, contour level = 0.014811 |
| 10592 | | |
| 10593 | | Position of copy of copy of A_fixed_model_2_A.pdb (#62) relative to |
| 10594 | | W20_J160_run_ct6_body001.mrc (#33.1) coordinates: |
| 10595 | | Matrix rotation and translation |
| 10596 | | -0.49233635 -0.83165726 -0.25680949 286.92215484 |
| 10597 | | -0.53267730 0.05454857 0.84455867 111.52201588 |
| 10598 | | -0.68837476 0.55260352 -0.46986119 217.04968179 |
| 10599 | | Axis -0.48600137 0.71840251 0.49769518 |
| 10600 | | Axis point 201.81367843 0.00000000 36.66306761 |
| 10601 | | Rotation angle (degrees) 162.52050103 |
| 10602 | | Shift along axis 48.69771641 |
| 10603 | | |
| 10604 | | |
| 10605 | | > show #!33 models |
| 10606 | | |
| 10607 | | > hide #!62 models |
| 10608 | | |
| 10609 | | > show #!62 models |
| 10610 | | |
| 10611 | | > hide #!62 models |
| 10612 | | |
| 10613 | | > show #!60 models |
| 10614 | | |
| 10615 | | > show #!61 models |
| 10616 | | |
| 10617 | | > hide #!61 models |
| 10618 | | |
| 10619 | | > show #!61 models |
| 10620 | | |
| 10621 | | > show #!62 models |
| 10622 | | |
| 10623 | | > hide #!62 models |
| 10624 | | |
| 10625 | | > show #!62 models |
| 10626 | | |
| 10627 | | > hide #!62 models |
| 10628 | | |
| 10629 | | > close #61 |
| 10630 | | |
| 10631 | | > show #!62 models |
| 10632 | | |
| 10633 | | > combine #62 |
| 10634 | | |
| 10635 | | > hide #!62 models |
| 10636 | | |
| 10637 | | > hide #!60 models |
| 10638 | | |
| 10639 | | > fitmap #61 inMap #33.2 |
| 10640 | | |
| 10641 | | Fit molecule copy of copy of copy of A_fixed_model_2_A.pdb (#61) to map |
| 10642 | | W20_J160_run_ct6_body002.mrc (#33.2) using 4038 atoms |
| 10643 | | average map value = 0.009974, steps = 196 |
| 10644 | | shifted from previous position = 3.26 |
| 10645 | | rotated from previous position = 10.9 degrees |
| 10646 | | atoms outside contour = 2744, contour level = 0.014809 |
| 10647 | | |
| 10648 | | Position of copy of copy of copy of A_fixed_model_2_A.pdb (#61) relative to |
| 10649 | | W20_J160_run_ct6_body002.mrc (#33.2) coordinates: |
| 10650 | | Matrix rotation and translation |
| 10651 | | -0.32106500 -0.91005872 -0.26212669 270.65425094 |
| 10652 | | -0.60680985 -0.01482211 0.79470882 127.10234227 |
| 10653 | | -0.72711696 0.41421424 -0.54747373 232.51552841 |
| 10654 | | Axis -0.56535652 0.69090415 0.45058125 |
| 10655 | | Axis point 214.25644692 0.00000000 38.06080511 |
| 10656 | | Rotation angle (degrees) 160.33570313 |
| 10657 | | Shift along axis 39.56652768 |
| 10658 | | |
| 10659 | | |
| 10660 | | > hide #!33 models |
| 10661 | | |
| 10662 | | > show #!33 models |
| 10663 | | |
| 10664 | | > hide #!33 models |
| 10665 | | |
| 10666 | | Drag select of 10 residues |
| 10667 | | |
| 10668 | | > select up |
| 10669 | | |
| 10670 | | 388 atoms, 400 bonds, 50 residues, 1 model selected |
| 10671 | | |
| 10672 | | > delete sel |
| 10673 | | |
| 10674 | | Drag select of 88 residues |
| 10675 | | |
| 10676 | | > select clear |
| 10677 | | |
| 10678 | | Drag select of 57 residues |
| 10679 | | |
| 10680 | | > select clear |
| 10681 | | |
| 10682 | | Drag select of 78 residues |
| 10683 | | |
| 10684 | | > select up |
| 10685 | | |
| 10686 | | 719 atoms, 728 bonds, 89 residues, 1 model selected |
| 10687 | | |
| 10688 | | > delete sel |
| 10689 | | |
| 10690 | | Drag select of 3 residues |
| 10691 | | |
| 10692 | | > select up |
| 10693 | | |
| 10694 | | 125 atoms, 126 bonds, 16 residues, 1 model selected |
| 10695 | | |
| 10696 | | > delete sel |
| 10697 | | |
| 10698 | | > select clear |
| 10699 | | |
| 10700 | | > select #61/A:670 |
| 10701 | | |
| 10702 | | 8 atoms, 7 bonds, 1 residue, 1 model selected |
| 10703 | | |
| 10704 | | > select clear |
| 10705 | | |
| 10706 | | Drag select of 2 residues |
| 10707 | | |
| 10708 | | > select clear |
| 10709 | | |
| 10710 | | Drag select of 28 residues |
| 10711 | | |
| 10712 | | > fitmap #62 inMap #33.1 |
| 10713 | | |
| 10714 | | Fit molecule copy of copy of A_fixed_model_2_A.pdb (#62) to map |
| 10715 | | W20_J160_run_ct6_body001.mrc (#33.1) using 4038 atoms |
| 10716 | | average map value = 0.007876, steps = 40 |
| 10717 | | shifted from previous position = 0.0144 |
| 10718 | | rotated from previous position = 0.0178 degrees |
| 10719 | | atoms outside contour = 3169, contour level = 0.014811 |
| 10720 | | |
| 10721 | | Position of copy of copy of A_fixed_model_2_A.pdb (#62) relative to |
| 10722 | | W20_J160_run_ct6_body001.mrc (#33.1) coordinates: |
| 10723 | | Matrix rotation and translation |
| 10724 | | -0.49227524 -0.83177636 -0.25654079 286.90681543 |
| 10725 | | -0.53253090 0.05466159 0.84464368 111.49096886 |
| 10726 | | -0.68853172 0.55241307 -0.46985516 217.06478146 |
| 10727 | | Axis -0.48599732 0.71842724 0.49766344 |
| 10728 | | Axis point 201.81610476 0.00000000 36.65534481 |
| 10729 | | Rotation angle (degrees) 162.50332601 |
| 10730 | | Shift along axis 48.68741004 |
| 10731 | | |
| 10732 | | |
| 10733 | | > delete sel |
| 10734 | | |
| 10735 | | > select clear |
| 10736 | | |
| 10737 | | > fitmap #61 inMap #33.2 |
| 10738 | | |
| 10739 | | Fit molecule copy of copy of copy of A_fixed_model_2_A.pdb (#61) to map |
| 10740 | | W20_J160_run_ct6_body002.mrc (#33.2) using 2593 atoms |
| 10741 | | average map value = 0.01536, steps = 44 |
| 10742 | | shifted from previous position = 0.0309 |
| 10743 | | rotated from previous position = 0.286 degrees |
| 10744 | | atoms outside contour = 1295, contour level = 0.014809 |
| 10745 | | |
| 10746 | | Position of copy of copy of copy of A_fixed_model_2_A.pdb (#61) relative to |
| 10747 | | W20_J160_run_ct6_body002.mrc (#33.2) coordinates: |
| 10748 | | Matrix rotation and translation |
| 10749 | | -0.31750805 -0.91177971 -0.26047341 270.11347052 |
| 10750 | | -0.60893731 -0.01451577 0.79308552 127.53984411 |
| 10751 | | -0.72690026 0.41042302 -0.55060781 232.93839308 |
| 10752 | | Axis -0.56686193 0.69094730 0.44861941 |
| 10753 | | Axis point 214.45195639 0.00000000 38.26217280 |
| 10754 | | Rotation angle (degrees) 160.27371795 |
| 10755 | | Shift along axis 39.50695274 |
| 10756 | | |
| 10757 | | |
| 10758 | | > show #!33 models |
| 10759 | | |
| 10760 | | > hide #!33 models |
| 10761 | | |
| 10762 | | > hide #!61 models |
| 10763 | | |
| 10764 | | > show #!61 models |
| 10765 | | |
| 10766 | | Drag select of 8 residues |
| 10767 | | |
| 10768 | | > select up |
| 10769 | | |
| 10770 | | 138 atoms, 140 bonds, 17 residues, 1 model selected |
| 10771 | | |
| 10772 | | > select clear |
| 10773 | | |
| 10774 | | Drag select of 19 residues |
| 10775 | | |
| 10776 | | > select up |
| 10777 | | |
| 10778 | | 196 atoms, 200 bonds, 25 residues, 1 model selected |
| 10779 | | |
| 10780 | | > select clear |
| 10781 | | |
| 10782 | | Drag select of 3 residues |
| 10783 | | |
| 10784 | | > select up |
| 10785 | | |
| 10786 | | 41 atoms, 42 bonds, 5 residues, 1 model selected |
| 10787 | | |
| 10788 | | > delete sel |
| 10789 | | |
| 10790 | | > select #61/A:637 |
| 10791 | | |
| 10792 | | 7 atoms, 6 bonds, 1 residue, 1 model selected |
| 10793 | | |
| 10794 | | > delete sel |
| 10795 | | |
| 10796 | | Drag select of 2 residues |
| 10797 | | |
| 10798 | | > delete sel |
| 10799 | | |
| 10800 | | Drag select of 9 residues |
| 10801 | | |
| 10802 | | > delete sel |
| 10803 | | |
| 10804 | | > select #61/A:625 |
| 10805 | | |
| 10806 | | 8 atoms, 7 bonds, 1 residue, 1 model selected |
| 10807 | | |
| 10808 | | > delete sel |
| 10809 | | |
| 10810 | | > select #61/A:624 |
| 10811 | | |
| 10812 | | 6 atoms, 5 bonds, 1 residue, 1 model selected |
| 10813 | | |
| 10814 | | > delete sel |
| 10815 | | |
| 10816 | | > select #61/A:623 |
| 10817 | | |
| 10818 | | 9 atoms, 8 bonds, 1 residue, 1 model selected |
| 10819 | | |
| 10820 | | > delete sel |
| 10821 | | |
| 10822 | | > select clear |
| 10823 | | |
| 10824 | | [Repeated 1 time(s)] |
| 10825 | | |
| 10826 | | > select #61/A:622 |
| 10827 | | |
| 10828 | | 4 atoms, 3 bonds, 1 residue, 1 model selected |
| 10829 | | |
| 10830 | | > delete sel |
| 10831 | | |
| 10832 | | > fitmap #61 inMap #33.2 |
| 10833 | | |
| 10834 | | Fit molecule copy of copy of copy of A_fixed_model_2_A.pdb (#61) to map |
| 10835 | | W20_J160_run_ct6_body002.mrc (#33.2) using 2426 atoms |
| 10836 | | average map value = 0.01573, steps = 64 |
| 10837 | | shifted from previous position = 0.0485 |
| 10838 | | rotated from previous position = 0.404 degrees |
| 10839 | | atoms outside contour = 1173, contour level = 0.014809 |
| 10840 | | |
| 10841 | | Position of copy of copy of copy of A_fixed_model_2_A.pdb (#61) relative to |
| 10842 | | W20_J160_run_ct6_body002.mrc (#33.2) coordinates: |
| 10843 | | Matrix rotation and translation |
| 10844 | | -0.31268611 -0.91441000 -0.25706370 269.24111705 |
| 10845 | | -0.61103650 -0.01356040 0.79148626 127.91459578 |
| 10846 | | -0.72722884 0.40456206 -0.55449774 233.52795858 |
| 10847 | | Axis -0.56875957 0.69111966 0.44594414 |
| 10848 | | Axis point 214.64229202 0.00000000 38.50676211 |
| 10849 | | Rotation angle (degrees) 160.11414397 |
| 10850 | | Shift along axis 39.41125523 |
| 10851 | | |
| 10852 | | |
| 10853 | | > show #!33 models |
| 10854 | | |
| 10855 | | > show #!62 models |
| 10856 | | |
| 10857 | | > hide #!62 models |
| 10858 | | |
| 10859 | | > show #!60 models |
| 10860 | | |
| 10861 | | > hide #!61 models |
| 10862 | | |
| 10863 | | > hide #!60 models |
| 10864 | | |
| 10865 | | > show #!62 models |
| 10866 | | |
| 10867 | | > hide #!33 models |
| 10868 | | |
| 10869 | | Drag select of 2 residues |
| 10870 | | |
| 10871 | | > select up |
| 10872 | | |
| 10873 | | 60 atoms, 60 bonds, 7 residues, 1 model selected |
| 10874 | | |
| 10875 | | > select up |
| 10876 | | |
| 10877 | | 4038 atoms, 4115 bonds, 507 residues, 1 model selected |
| 10878 | | |
| 10879 | | > select down |
| 10880 | | |
| 10881 | | 60 atoms, 60 bonds, 7 residues, 1 model selected |
| 10882 | | |
| 10883 | | > select clear |
| 10884 | | |
| 10885 | | Drag select of 245 residues |
| 10886 | | |
| 10887 | | > select up |
| 10888 | | |
| 10889 | | 2197 atoms, 2238 bonds, 276 residues, 1 model selected |
| 10890 | | |
| 10891 | | > delete sel |
| 10892 | | |
| 10893 | | Drag select of 6 residues, 2 pseudobonds |
| 10894 | | |
| 10895 | | > select clear |
| 10896 | | |
| 10897 | | Drag select of 4 residues |
| 10898 | | |
| 10899 | | > select up |
| 10900 | | |
| 10901 | | 1841 atoms, 1872 bonds, 231 residues, 1 model selected |
| 10902 | | |
| 10903 | | > select down |
| 10904 | | |
| 10905 | | 36 atoms, 4 residues, 1 model selected |
| 10906 | | |
| 10907 | | > delete sel |
| 10908 | | |
| 10909 | | Drag select of 6 residues |
| 10910 | | |
| 10911 | | > delete sel |
| 10912 | | |
| 10913 | | Drag select of 12 residues |
| 10914 | | |
| 10915 | | > select up |
| 10916 | | |
| 10917 | | 116 atoms, 117 bonds, 13 residues, 1 model selected |
| 10918 | | |
| 10919 | | > delete sel |
| 10920 | | |
| 10921 | | > select clear |
| 10922 | | |
| 10923 | | [Repeated 2 time(s)] |
| 10924 | | |
| 10925 | | > select #62/A:618 |
| 10926 | | |
| 10927 | | 5 atoms, 4 bonds, 1 residue, 1 model selected |
| 10928 | | |
| 10929 | | > delete sel |
| 10930 | | |
| 10931 | | > select clear |
| 10932 | | |
| 10933 | | > select #62/A:619 |
| 10934 | | |
| 10935 | | 9 atoms, 8 bonds, 1 residue, 1 model selected |
| 10936 | | |
| 10937 | | > delete sel |
| 10938 | | |
| 10939 | | > select clear |
| 10940 | | |
| 10941 | | > select #62/A:620 |
| 10942 | | |
| 10943 | | 8 atoms, 7 bonds, 1 residue, 1 model selected |
| 10944 | | |
| 10945 | | > delete sel |
| 10946 | | |
| 10947 | | > select #62/A:621 |
| 10948 | | |
| 10949 | | 7 atoms, 6 bonds, 1 residue, 1 model selected |
| 10950 | | |
| 10951 | | > delete sel |
| 10952 | | |
| 10953 | | > fitmap #62 inMap #33.1 |
| 10954 | | |
| 10955 | | Fit molecule copy of copy of A_fixed_model_2_A.pdb (#62) to map |
| 10956 | | W20_J160_run_ct6_body001.mrc (#33.1) using 1612 atoms |
| 10957 | | average map value = 0.01422, steps = 100 |
| 10958 | | shifted from previous position = 1.05 |
| 10959 | | rotated from previous position = 7.03 degrees |
| 10960 | | atoms outside contour = 871, contour level = 0.014811 |
| 10961 | | |
| 10962 | | Position of copy of copy of A_fixed_model_2_A.pdb (#62) relative to |
| 10963 | | W20_J160_run_ct6_body001.mrc (#33.1) coordinates: |
| 10964 | | Matrix rotation and translation |
| 10965 | | -0.56465803 -0.76120461 -0.31894961 292.13379963 |
| 10966 | | -0.55687251 0.06616556 0.82795841 113.21112709 |
| 10967 | | -0.60914228 0.64512763 -0.46125484 203.93497656 |
| 10968 | | Axis -0.45795004 0.72686749 0.51180603 |
| 10969 | | Axis point 193.99705310 0.00000000 40.16949466 |
| 10970 | | Rotation angle (degrees) 168.48533814 |
| 10971 | | Shift along axis 52.88195186 |
| 10972 | | |
| 10973 | | |
| 10974 | | > show #!33 models |
| 10975 | | |
| 10976 | | > show #!61 models |
| 10977 | | |
| 10978 | | > show #!60 models |
| 10979 | | |
| 10980 | | > save "/Users/becca/Desktop/Postdoc/COPI notebooks and |
| 10981 | | > resources/Processing/W20_MM_cyto_COPI/20250704_W20_COPI_cyto_alignments.cxs" |
| 10982 | | |
| 10983 | | ——— End of log from Fri Jul 4 09:53:55 2025 ——— |
| 10984 | | |
| 10985 | | opened ChimeraX session |
| 10986 | | |
| 10987 | | > hide #!33 models |
| 10988 | | |
| 10989 | | > show #!33 models |
| 10990 | | |
| 10991 | | > open |
| 10992 | | > /Users/becca/Desktop/Postdoc/Phenix/W20_MM_cyto_COPI/PredictAndBuildCryoEM_1/PredictAndBuild_1_overall_best_map.ccp4 |
| 10993 | | |
| 10994 | | Opened PredictAndBuild_1_overall_best_map.ccp4 as #63, grid size 138,147,182, |
| 10995 | | pixel 1.2, shown at level 0.422, step 1, values float32 |
| 10996 | | |
| 10997 | | > select add #63 |
| 10998 | | |
| 10999 | | 2 models selected |
| 11000 | | |
| 11001 | | > select subtract #63 |
| 11002 | | |
| 11003 | | Nothing selected |
| 11004 | | |
| 11005 | | > hide #!33 models |
| 11006 | | |
| 11007 | | > volume #63 level 0.2752 |
| 11008 | | |
| 11009 | | > toolshed show |
| 11010 | | |
| 11011 | | Downloading bundle ChimeraX_locscalesurfer-0.1.1-py3-none-any.whl |
| 11012 | | Downloading bundle ChimeraX_ISOLDE-1.9-cp311-cp311-macosx_10_13_universal2.whl |
| 11013 | | [Repeated 1 time(s)] |
| 11014 | | |
| 11015 | | > select add #63 |
| 11016 | | |
| 11017 | | 2 models selected |
| 11018 | | |
| 11019 | | > show #!1 models |
| 11020 | | |
| 11021 | | > ui mousemode right "rotate selected models" |
| 11022 | | |
| 11023 | | > view matrix models |
| 11024 | | > #63,-0.58537,-0.79697,-0.1489,345.68,-0.68073,0.38338,0.6242,89.496,-0.44039,0.46675,-0.76694,243.81 |
| 11025 | | |
| 11026 | | > fitmap #63 inMap #33.2 |
| 11027 | | |
| 11028 | | Fit map PredictAndBuild_1_overall_best_map.ccp4 in map |
| 11029 | | W20_J160_run_ct6_body002.mrc using 134378 points |
| 11030 | | correlation = 0.4, correlation about mean = 0.0294, overlap = 163.4 |
| 11031 | | steps = 156, shift = 19.7, angle = 10.1 degrees |
| 11032 | | |
| 11033 | | Position of PredictAndBuild_1_overall_best_map.ccp4 (#63) relative to |
| 11034 | | W20_J160_run_ct6_body002.mrc (#33.2) coordinates: |
| 11035 | | Matrix rotation and translation |
| 11036 | | -0.49730946 -0.85158343 -0.16579799 356.32016493 |
| 11037 | | -0.56399341 0.17211906 0.80764255 80.81400003 |
| 11038 | | -0.65923801 0.49515725 -0.56588387 229.61138609 |
| 11039 | | Axis -0.47998711 0.75793917 0.44174720 |
| 11040 | | Axis point 229.49905252 0.00000000 52.36918700 |
| 11041 | | Rotation angle (degrees) 161.00326601 |
| 11042 | | Shift along axis -8.34680262 |
| 11043 | | |
| 11044 | | |
| 11045 | | > fitmap #63 inMap #2 |
| 11046 | | |
| 11047 | | Fit map PredictAndBuild_1_overall_best_map.ccp4 in map |
| 11048 | | W20_J3623_consensus_structure.mrc using 134378 points |
| 11049 | | correlation = 0.9819, correlation about mean = 0.7973, overlap = 2.007e+04 |
| 11050 | | steps = 112, shift = 10.7, angle = 7.31 degrees |
| 11051 | | |
| 11052 | | Position of PredictAndBuild_1_overall_best_map.ccp4 (#63) relative to |
| 11053 | | W20_J3623_consensus_structure.mrc (#2) coordinates: |
| 11054 | | Matrix rotation and translation |
| 11055 | | -0.34010494 -0.91904138 -0.19922745 353.84532994 |
| 11056 | | -0.77653019 0.15497541 0.61072375 146.03986464 |
| 11057 | | -0.53040504 0.36241629 -0.76637128 277.65026005 |
| 11058 | | Axis -0.56720681 0.75650641 0.32553722 |
| 11059 | | Axis point 251.99725576 0.00000000 81.48732765 |
| 11060 | | Rotation angle (degrees) 167.35637881 |
| 11061 | | Shift along axis 0.16210555 |
| 11062 | | |
| 11063 | | |
| 11064 | | > select subtract #63 |
| 11065 | | |
| 11066 | | Nothing selected |
| 11067 | | |
| 11068 | | > hide #!1 models |
| 11069 | | |
| 11070 | | > show #!11 models |
| 11071 | | |
| 11072 | | > hide #!11 models |
| 11073 | | |
| 11074 | | > show #!11 models |
| 11075 | | |
| 11076 | | > hide #!63 models |
| 11077 | | |
| 11078 | | > show #!63 models |
| 11079 | | |
| 11080 | | > hide #!11 models |
| 11081 | | |
| 11082 | | > show #!8 models |
| 11083 | | |
| 11084 | | > hide #!8 models |
| 11085 | | |
| 11086 | | > show #!7 models |
| 11087 | | |
| 11088 | | > hide #!63 models |
| 11089 | | |
| 11090 | | > show #!63 models |
| 11091 | | |
| 11092 | | > hide #!7 models |
| 11093 | | |
| 11094 | | > show #!7 models |
| 11095 | | |
| 11096 | | > hide #!7 models |
| 11097 | | |
| 11098 | | > show #!7 models |
| 11099 | | |
| 11100 | | > close #63 |
| 11101 | | |
| 11102 | | > open |
| 11103 | | > /Users/becca/Desktop/Postdoc/Phenix/W20_MM_cyto_COPI/PredictAndBuildCryoEM_1/PredictAndBuild_1_overall_best_superposed_predicted_models.pdb |
| 11104 | | |
| 11105 | | Chain information for |
| 11106 | | PredictAndBuild_1_overall_best_superposed_predicted_models.pdb #63 |
| 11107 | | --- |
| 11108 | | Chain | Description |
| 11109 | | A | No description available |
| 11110 | | B | No description available |
| 11111 | | F | No description available |
| 11112 | | |
| 11113 | | Computing secondary structure |
| 11114 | | |
| 11115 | | > open |
| 11116 | | > /Users/becca/Desktop/Postdoc/Phenix/W20_MM_cyto_COPI/PredictAndBuildCryoEM_1/PredictAndBuild_1_overall_best.pdb |
| 11117 | | |
| 11118 | | Chain information for PredictAndBuild_1_overall_best.pdb #64 |
| 11119 | | --- |
| 11120 | | Chain | Description |
| 11121 | | A | No description available |
| 11122 | | B | No description available |
| 11123 | | C | No description available |
| 11124 | | D | No description available |
| 11125 | | F | No description available |
| 11126 | | |
| 11127 | | |
| 11128 | | > open |
| 11129 | | > /Users/becca/Desktop/Postdoc/Phenix/W20_MM_cyto_COPI/PredictAndBuildCryoEM_1/PredictAndBuild_1_superposed_predicted_untrimmed_cycle_1.pdb |
| 11130 | | |
| 11131 | | Chain information for |
| 11132 | | PredictAndBuild_1_superposed_predicted_untrimmed_cycle_1.pdb #65 |
| 11133 | | --- |
| 11134 | | Chain | Description |
| 11135 | | A | No description available |
| 11136 | | B | No description available |
| 11137 | | F | No description available |
| 11138 | | |
| 11139 | | Computing secondary structure |
| 11140 | | |
| 11141 | | > select add #63 |
| 11142 | | |
| 11143 | | 13443 atoms, 13688 bonds, 16 pseudobonds, 1678 residues, 2 models selected |
| 11144 | | |
| 11145 | | > select add #64 |
| 11146 | | |
| 11147 | | 34205 atoms, 34848 bonds, 30 pseudobonds, 4274 residues, 4 models selected |
| 11148 | | |
| 11149 | | > select add #65 |
| 11150 | | |
| 11151 | | 52865 atoms, 53866 bonds, 30 pseudobonds, 6628 residues, 5 models selected |
| 11152 | | |
| 11153 | | > hide sel atoms |
| 11154 | | |
| 11155 | | > show sel cartoons |
| 11156 | | |
| 11157 | | Computing secondary structure |
| 11158 | | |
| 11159 | | > select subtract #65 |
| 11160 | | |
| 11161 | | 34205 atoms, 34848 bonds, 30 pseudobonds, 4274 residues, 4 models selected |
| 11162 | | |
| 11163 | | > select subtract #64 |
| 11164 | | |
| 11165 | | 13443 atoms, 13688 bonds, 16 pseudobonds, 1678 residues, 2 models selected |
| 11166 | | |
| 11167 | | > select subtract #63 |
| 11168 | | |
| 11169 | | Nothing selected |
| 11170 | | |
| 11171 | | > select add #63 |
| 11172 | | |
| 11173 | | 13443 atoms, 13688 bonds, 16 pseudobonds, 1678 residues, 2 models selected |
| 11174 | | |
| 11175 | | > select add #64 |
| 11176 | | |
| 11177 | | 34205 atoms, 34848 bonds, 30 pseudobonds, 4274 residues, 4 models selected |
| 11178 | | |
| 11179 | | > select add #65 |
| 11180 | | |
| 11181 | | 52865 atoms, 53866 bonds, 30 pseudobonds, 6628 residues, 5 models selected |
| 11182 | | |
| 11183 | | > view matrix models |
| 11184 | | > #63,-0.079462,-0.96928,-0.23276,330.08,-0.99279,0.055943,0.10597,250.15,-0.089693,0.2395,-0.96674,313.02,#64,-0.079462,-0.96928,-0.23276,330.08,-0.99279,0.055943,0.10597,250.15,-0.089693,0.2395,-0.96674,313.02,#65,-0.079462,-0.96928,-0.23276,330.08,-0.99279,0.055943,0.10597,250.15,-0.089693,0.2395,-0.96674,313.02 |
| 11185 | | |
| 11186 | | > ui mousemode right "translate selected models" |
| 11187 | | |
| 11188 | | > view matrix models |
| 11189 | | > #63,-0.079462,-0.96928,-0.23276,336.58,-0.99279,0.055943,0.10597,275.32,-0.089693,0.2395,-0.96674,259.8,#64,-0.079462,-0.96928,-0.23276,336.58,-0.99279,0.055943,0.10597,275.32,-0.089693,0.2395,-0.96674,259.8,#65,-0.079462,-0.96928,-0.23276,336.58,-0.99279,0.055943,0.10597,275.32,-0.089693,0.2395,-0.96674,259.8 |
| 11190 | | |
| 11191 | | > view matrix models |
| 11192 | | > #63,-0.079462,-0.96928,-0.23276,335,-0.99279,0.055943,0.10597,254.69,-0.089693,0.2395,-0.96674,251.24,#64,-0.079462,-0.96928,-0.23276,335,-0.99279,0.055943,0.10597,254.69,-0.089693,0.2395,-0.96674,251.24,#65,-0.079462,-0.96928,-0.23276,335,-0.99279,0.055943,0.10597,254.69,-0.089693,0.2395,-0.96674,251.24 |
| 11193 | | |
| 11194 | | > ui mousemode right "rotate selected models" |
| 11195 | | |
| 11196 | | > view matrix models |
| 11197 | | > #63,-0.095152,-0.88164,0.46222,203.93,-0.95238,0.21575,0.21547,206.02,-0.28969,-0.41971,-0.86019,359.38,#64,-0.095152,-0.88164,0.46222,203.93,-0.95238,0.21575,0.21547,206.02,-0.28969,-0.41971,-0.86019,359.38,#65,-0.095152,-0.88164,0.46222,203.93,-0.95238,0.21575,0.21547,206.02,-0.28969,-0.41971,-0.86019,359.38 |
| 11198 | | |
| 11199 | | > ui mousemode right "translate selected models" |
| 11200 | | |
| 11201 | | > view matrix models |
| 11202 | | > #63,-0.095152,-0.88164,0.46222,227.08,-0.95238,0.21575,0.21547,215.24,-0.28969,-0.41971,-0.86019,381.48,#64,-0.095152,-0.88164,0.46222,227.08,-0.95238,0.21575,0.21547,215.24,-0.28969,-0.41971,-0.86019,381.48,#65,-0.095152,-0.88164,0.46222,227.08,-0.95238,0.21575,0.21547,215.24,-0.28969,-0.41971,-0.86019,381.48 |
| 11203 | | |
| 11204 | | > hide #!7 models |
| 11205 | | |
| 11206 | | > show #!33 models |
| 11207 | | |
| 11208 | | > select subtract #65 |
| 11209 | | |
| 11210 | | 34205 atoms, 34848 bonds, 30 pseudobonds, 4274 residues, 4 models selected |
| 11211 | | |
| 11212 | | > select subtract #64 |
| 11213 | | |
| 11214 | | 13443 atoms, 13688 bonds, 16 pseudobonds, 1678 residues, 2 models selected |
| 11215 | | |
| 11216 | | > select subtract #63 |
| 11217 | | |
| 11218 | | Nothing selected |
| 11219 | | |
| 11220 | | > hide #65 models |
| 11221 | | |
| 11222 | | > hide #!64 models |
| 11223 | | |
| 11224 | | > fitmap #63 inMap #2 |
| 11225 | | |
| 11226 | | Fit molecule PredictAndBuild_1_overall_best_superposed_predicted_models.pdb |
| 11227 | | (#63) to map W20_J3623_consensus_structure.mrc (#2) using 13443 atoms |
| 11228 | | average map value = 0.3311, steps = 268 |
| 11229 | | shifted from previous position = 24.1 |
| 11230 | | rotated from previous position = 48.9 degrees |
| 11231 | | atoms outside contour = 4479, contour level = 0.25746 |
| 11232 | | |
| 11233 | | Position of PredictAndBuild_1_overall_best_superposed_predicted_models.pdb |
| 11234 | | (#63) relative to W20_J3623_consensus_structure.mrc (#2) coordinates: |
| 11235 | | Matrix rotation and translation |
| 11236 | | -0.33968216 -0.91923759 -0.19904344 353.65205126 |
| 11237 | | -0.77596080 0.15430364 0.61161690 145.90271071 |
| 11238 | | -0.53150812 0.36220526 -0.76570653 277.79017130 |
| 11239 | | Axis -0.56733253 0.75625188 0.32590934 |
| 11240 | | Axis point 251.98315191 0.00000000 81.43051773 |
| 11241 | | Rotation angle (degrees) 167.30207874 |
| 11242 | | Shift along axis 0.23529698 |
| 11243 | | |
| 11244 | | |
| 11245 | | > hide #!61 models |
| 11246 | | |
| 11247 | | > hide #!62 models |
| 11248 | | |
| 11249 | | > hide #!60 models |
| 11250 | | |
| 11251 | | > hide #!63 models |
| 11252 | | |
| 11253 | | > show #!63 models |
| 11254 | | |
| 11255 | | > hide #!63 models |
| 11256 | | |
| 11257 | | > show #!64 models |
| 11258 | | |
| 11259 | | > fitmap #64 inMap #2 |
| 11260 | | |
| 11261 | | Fit molecule PredictAndBuild_1_overall_best.pdb (#64) to map |
| 11262 | | W20_J3623_consensus_structure.mrc (#2) using 20762 atoms |
| 11263 | | average map value = 0.2661, steps = 552 |
| 11264 | | shifted from previous position = 15.4 |
| 11265 | | rotated from previous position = 48.8 degrees |
| 11266 | | atoms outside contour = 10565, contour level = 0.25746 |
| 11267 | | |
| 11268 | | Position of PredictAndBuild_1_overall_best.pdb (#64) relative to |
| 11269 | | W20_J3623_consensus_structure.mrc (#2) coordinates: |
| 11270 | | Matrix rotation and translation |
| 11271 | | -0.33968228 -0.91946019 -0.19801243 353.57410808 |
| 11272 | | -0.77672233 0.15550766 0.61034400 145.97075470 |
| 11273 | | -0.53039456 0.36112372 -0.76698845 277.98078879 |
| 11274 | | Axis -0.56734428 0.75666030 0.32493947 |
| 11275 | | Axis point 251.92912923 0.00000000 81.66482425 |
| 11276 | | Rotation angle (degrees) 167.31225097 |
| 11277 | | Shift along axis 0.17895697 |
| 11278 | | |
| 11279 | | |
| 11280 | | > hide #!64 models |
| 11281 | | |
| 11282 | | > show #65 models |
| 11283 | | |
| 11284 | | > fitmap #65 inMap #2 |
| 11285 | | |
| 11286 | | Fit molecule PredictAndBuild_1_superposed_predicted_untrimmed_cycle_1.pdb |
| 11287 | | (#65) to map W20_J3623_consensus_structure.mrc (#2) using 18660 atoms |
| 11288 | | average map value = 0.2436, steps = 340 |
| 11289 | | shifted from previous position = 22.1 |
| 11290 | | rotated from previous position = 48.8 degrees |
| 11291 | | atoms outside contour = 9859, contour level = 0.25746 |
| 11292 | | |
| 11293 | | Position of PredictAndBuild_1_superposed_predicted_untrimmed_cycle_1.pdb (#65) |
| 11294 | | relative to W20_J3623_consensus_structure.mrc (#2) coordinates: |
| 11295 | | Matrix rotation and translation |
| 11296 | | -0.34475277 -0.91893726 -0.19157204 353.38290459 |
| 11297 | | -0.77178767 0.16132165 0.61507653 143.76727401 |
| 11298 | | -0.53431202 0.35990227 -0.76484053 278.30475415 |
| 11299 | | Axis -0.56463658 0.75839755 0.32560511 |
| 11300 | | Axis point 251.20855212 0.00000000 81.61803612 |
| 11301 | | Rotation angle (degrees) 166.94047161 |
| 11302 | | Shift along axis 0.11728580 |
| 11303 | | |
| 11304 | | |
| 11305 | | > hide #65 models |
| 11306 | | |
| 11307 | | Downloading bundle ChimeraX_ISOLDE-1.9-cp311-cp311-macosx_10_13_universal2.whl |
| 11308 | | |
| 11309 | | > save "/Users/becca/Desktop/Postdoc/COPI notebooks and |
| 11310 | | > resources/Processing/W20_MM_cyto_COPI/20250704_W20_COPI_cyto_alignments.cxs" |
| 11311 | | |
| 11312 | | ——— End of log from Fri Jul 4 10:54:24 2025 ——— |
| 11313 | | |
| 11314 | | opened ChimeraX session |
| 11315 | | |
| 11316 | | > show #!46 models |
| 11317 | | |
| 11318 | | > hide #!46 models |
| 11319 | | |
| 11320 | | > hide #!50 models |
| 11321 | | |
| 11322 | | > show #!50 models |
| 11323 | | |
| 11324 | | > show #!60 models |
| 11325 | | |
| 11326 | | > hide #!60 models |
| 11327 | | |
| 11328 | | > show #!60 models |
| 11329 | | |
| 11330 | | > show #!61 models |
| 11331 | | |
| 11332 | | > hide #!61 models |
| 11333 | | |
| 11334 | | > show #!61 models |
| 11335 | | |
| 11336 | | > show #!62 models |
| 11337 | | |
| 11338 | | > color #60 #61 #62 navy |
| 11339 | | |
| 11340 | | > hide #!62 models |
| 11341 | | |
| 11342 | | > hide #!61 models |
| 11343 | | |
| 11344 | | > hide #!60 models |
| 11345 | | |
| 11346 | | > show #!60 models |
| 11347 | | |
| 11348 | | > show #!61 models |
| 11349 | | |
| 11350 | | > show #!62 models |
| 11351 | | |
| 11352 | | > close #57 |
| 11353 | | |
| 11354 | | > close #58 |
| 11355 | | |
| 11356 | | > close #59 |
| 11357 | | |
| 11358 | | > show #51 models |
| 11359 | | |
| 11360 | | > hide #51 models |
| 11361 | | |
| 11362 | | > close #51 |
| 11363 | | |
| 11364 | | > show #52 models |
| 11365 | | |
| 11366 | | > hide #!62 models |
| 11367 | | |
| 11368 | | > hide #!61 models |
| 11369 | | |
| 11370 | | > hide #!60 models |
| 11371 | | |
| 11372 | | > hide #!33 models |
| 11373 | | |
| 11374 | | > show #35 models |
| 11375 | | |
| 11376 | | > hide #35 models |
| 11377 | | |
| 11378 | | > combine #52 |
| 11379 | | |
| 11380 | | > hide #52 models |
| 11381 | | |
| 11382 | | > ui mousemode right select |
| 11383 | | |
| 11384 | | > select clear |
| 11385 | | |
| 11386 | | > select #51/B:953 |
| 11387 | | |
| 11388 | | 8 atoms, 7 bonds, 1 residue, 1 model selected |
| 11389 | | |
| 11390 | | > select up |
| 11391 | | |
| 11392 | | 54 atoms, 53 bonds, 7 residues, 1 model selected |
| 11393 | | |
| 11394 | | > select up |
| 11395 | | |
| 11396 | | 7500 atoms, 7618 bonds, 953 residues, 1 model selected |
| 11397 | | |
| 11398 | | > select down |
| 11399 | | |
| 11400 | | 54 atoms, 53 bonds, 7 residues, 1 model selected |
| 11401 | | |
| 11402 | | > select clear |
| 11403 | | |
| 11404 | | Drag select of 76 residues |
| 11405 | | |
| 11406 | | > select up |
| 11407 | | |
| 11408 | | 756 atoms, 763 bonds, 99 residues, 1 model selected |
| 11409 | | |
| 11410 | | > select clear |
| 11411 | | |
| 11412 | | Drag select of 58 residues |
| 11413 | | |
| 11414 | | > select up |
| 11415 | | |
| 11416 | | 773 atoms, 785 bonds, 99 residues, 1 model selected |
| 11417 | | |
| 11418 | | > delete sel |
| 11419 | | |
| 11420 | | Drag select of 7 residues |
| 11421 | | |
| 11422 | | > delete sel |
| 11423 | | |
| 11424 | | > select clear |
| 11425 | | |
| 11426 | | Drag select of 10 residues, 2 pseudobonds |
| 11427 | | |
| 11428 | | > select up |
| 11429 | | |
| 11430 | | 110 atoms, 109 bonds, 2 pseudobonds, 15 residues, 2 models selected |
| 11431 | | |
| 11432 | | > delete sel |
| 11433 | | |
| 11434 | | Drag select of 62 residues |
| 11435 | | |
| 11436 | | > select up |
| 11437 | | |
| 11438 | | 883 atoms, 897 bonds, 115 residues, 1 model selected |
| 11439 | | |
| 11440 | | > delete sel |
| 11441 | | |
| 11442 | | Drag select of 24 residues, 1 pseudobonds |
| 11443 | | |
| 11444 | | > delete sel |
| 11445 | | |
| 11446 | | Drag select of 2 residues |
| 11447 | | |
| 11448 | | > delete sel |
| 11449 | | |
| 11450 | | > show #!33 models |
| 11451 | | |
| 11452 | | > hide #!33 models |
| 11453 | | |
| 11454 | | Drag select of 13 residues |
| 11455 | | |
| 11456 | | > select up |
| 11457 | | |
| 11458 | | 126 atoms, 126 bonds, 17 residues, 1 model selected |
| 11459 | | |
| 11460 | | > delete sel |
| 11461 | | |
| 11462 | | Drag select of 7 residues |
| 11463 | | |
| 11464 | | > select up |
| 11465 | | |
| 11466 | | 69 atoms, 69 bonds, 9 residues, 1 model selected |
| 11467 | | |
| 11468 | | > delete sel |
| 11469 | | |
| 11470 | | > show #!33 models |
| 11471 | | |
| 11472 | | > select #51/B:664 |
| 11473 | | |
| 11474 | | 7 atoms, 6 bonds, 1 residue, 1 model selected |
| 11475 | | |
| 11476 | | > select up |
| 11477 | | |
| 11478 | | 215 atoms, 217 bonds, 27 residues, 1 model selected |
| 11479 | | |
| 11480 | | > delete sel |
| 11481 | | |
| 11482 | | > select clear |
| 11483 | | |
| 11484 | | > select #51/B:521 |
| 11485 | | |
| 11486 | | 12 atoms, 12 bonds, 1 residue, 1 model selected |
| 11487 | | |
| 11488 | | > select up |
| 11489 | | |
| 11490 | | 334 atoms, 338 bonds, 44 residues, 1 model selected |
| 11491 | | |
| 11492 | | > delete sel |
| 11493 | | |
| 11494 | | > fitmap #51 inMap #33.1 |
| 11495 | | |
| 11496 | | Fit molecule copy of B_fixed_model_2_B.pdb (#51) to map |
| 11497 | | W20_J160_run_ct6_body001.mrc (#33.1) using 4745 atoms |
| 11498 | | average map value = 0.01558, steps = 60 |
| 11499 | | shifted from previous position = 0.386 |
| 11500 | | rotated from previous position = 0.444 degrees |
| 11501 | | atoms outside contour = 2213, contour level = 0.014811 |
| 11502 | | |
| 11503 | | Position of copy of B_fixed_model_2_B.pdb (#51) relative to |
| 11504 | | W20_J160_run_ct6_body001.mrc (#33.1) coordinates: |
| 11505 | | Matrix rotation and translation |
| 11506 | | -0.39489665 -0.88949565 -0.22990026 277.26110723 |
| 11507 | | -0.63858809 0.08584769 0.76474533 131.14250865 |
| 11508 | | -0.66050124 0.44880693 -0.60192230 226.00577301 |
| 11509 | | Axis -0.53542046 0.72973902 0.42521276 |
| 11510 | | Axis point 208.19952618 0.00000000 43.73317487 |
| 11511 | | Rotation angle (degrees) 162.84020444 |
| 11512 | | Shift along axis 43.34907536 |
| 11513 | | |
| 11514 | | |
| 11515 | | > hide #!51 models |
| 11516 | | |
| 11517 | | > show #52 models |
| 11518 | | |
| 11519 | | > hide #!50 models |
| 11520 | | |
| 11521 | | > show #!50 models |
| 11522 | | |
| 11523 | | > hide #!33 models |
| 11524 | | |
| 11525 | | Drag select of 161 residues |
| 11526 | | |
| 11527 | | > select up |
| 11528 | | |
| 11529 | | 1472 atoms, 1497 bonds, 183 residues, 1 model selected |
| 11530 | | |
| 11531 | | > delete sel |
| 11532 | | |
| 11533 | | Drag select of 68 residues, 3 pseudobonds |
| 11534 | | |
| 11535 | | > select clear |
| 11536 | | |
| 11537 | | Drag select of 90 residues, 3 pseudobonds |
| 11538 | | |
| 11539 | | > select up |
| 11540 | | |
| 11541 | | 934 atoms, 946 bonds, 3 pseudobonds, 118 residues, 2 models selected |
| 11542 | | |
| 11543 | | > delete sel |
| 11544 | | |
| 11545 | | Drag select of 208 residues |
| 11546 | | |
| 11547 | | > select up |
| 11548 | | |
| 11549 | | 2197 atoms, 2217 bonds, 276 residues, 1 model selected |
| 11550 | | |
| 11551 | | > delete sel |
| 11552 | | |
| 11553 | | Drag select of 109 residues, 2 pseudobonds |
| 11554 | | |
| 11555 | | > select clear |
| 11556 | | |
| 11557 | | Drag select of 98 residues, 2 pseudobonds |
| 11558 | | |
| 11559 | | > select clear |
| 11560 | | |
| 11561 | | > select #52/B:624 |
| 11562 | | |
| 11563 | | 6 atoms, 5 bonds, 1 residue, 1 model selected |
| 11564 | | Drag select of 109 residues, 2 pseudobonds |
| 11565 | | |
| 11566 | | > select up |
| 11567 | | |
| 11568 | | 854 atoms, 863 bonds, 2 pseudobonds, 110 residues, 2 models selected |
| 11569 | | |
| 11570 | | > select down |
| 11571 | | |
| 11572 | | 844 atoms, 2 pseudobonds, 109 residues, 2 models selected |
| 11573 | | |
| 11574 | | > select clear |
| 11575 | | |
| 11576 | | Drag select of 109 residues, 2 pseudobonds |
| 11577 | | |
| 11578 | | > select up |
| 11579 | | |
| 11580 | | 854 atoms, 863 bonds, 2 pseudobonds, 110 residues, 2 models selected |
| 11581 | | |
| 11582 | | > delete sel |
| 11583 | | |
| 11584 | | > show #!33 models |
| 11585 | | |
| 11586 | | > show #!37 models |
| 11587 | | |
| 11588 | | > hide #!37 models |
| 11589 | | |
| 11590 | | > show #!37 models |
| 11591 | | |
| 11592 | | > hide #!37 models |
| 11593 | | |
| 11594 | | > hide #!33 models |
| 11595 | | |
| 11596 | | Drag select of 3 residues |
| 11597 | | |
| 11598 | | > select up |
| 11599 | | |
| 11600 | | 69 atoms, 69 bonds, 9 residues, 1 model selected |
| 11601 | | |
| 11602 | | > delete sel |
| 11603 | | |
| 11604 | | Drag select of 1 residues |
| 11605 | | |
| 11606 | | > select clear |
| 11607 | | |
| 11608 | | [Repeated 1 time(s)]Drag select of 2 residues |
| 11609 | | |
| 11610 | | > select up |
| 11611 | | |
| 11612 | | 98 atoms, 98 bonds, 13 residues, 1 model selected |
| 11613 | | |
| 11614 | | > select down |
| 11615 | | |
| 11616 | | 12 atoms, 2 residues, 1 model selected |
| 11617 | | |
| 11618 | | > select up |
| 11619 | | |
| 11620 | | 98 atoms, 98 bonds, 13 residues, 1 model selected |
| 11621 | | |
| 11622 | | > delete sel |
| 11623 | | |
| 11624 | | > show #!33 models |
| 11625 | | |
| 11626 | | > fitmap #62 inMap #33.1 |
| 11627 | | |
| 11628 | | Fit molecule copy of copy of A_fixed_model_2_A.pdb (#62) to map |
| 11629 | | W20_J160_run_ct6_body001.mrc (#33.1) using 1612 atoms |
| 11630 | | average map value = 0.01422, steps = 44 |
| 11631 | | shifted from previous position = 0.0171 |
| 11632 | | rotated from previous position = 0.0202 degrees |
| 11633 | | atoms outside contour = 870, contour level = 0.014811 |
| 11634 | | |
| 11635 | | Position of copy of copy of A_fixed_model_2_A.pdb (#62) relative to |
| 11636 | | W20_J160_run_ct6_body001.mrc (#33.1) coordinates: |
| 11637 | | Matrix rotation and translation |
| 11638 | | -0.56449972 -0.76141548 -0.31872643 292.10636189 |
| 11639 | | -0.55678517 0.06618546 0.82801555 113.19196963 |
| 11640 | | -0.60936880 0.64487670 -0.46130652 203.97357809 |
| 11641 | | Axis -0.45800977 0.72686404 0.51175748 |
| 11642 | | Axis point 194.00483060 0.00000000 40.16620251 |
| 11643 | | Rotation angle (degrees) 168.46719367 |
| 11644 | | Shift along axis 52.87260780 |
| 11645 | | |
| 11646 | | |
| 11647 | | > fitmap #52 inMap #33.1 |
| 11648 | | |
| 11649 | | Fit molecule B_fixed_model_2_B.pdb (#52) to map W20_J160_run_ct6_body001.mrc |
| 11650 | | (#33.1) using 1876 atoms |
| 11651 | | average map value = 0.01465, steps = 68 |
| 11652 | | shifted from previous position = 1.95 |
| 11653 | | rotated from previous position = 6.84 degrees |
| 11654 | | atoms outside contour = 924, contour level = 0.014811 |
| 11655 | | |
| 11656 | | Position of B_fixed_model_2_B.pdb (#52) relative to |
| 11657 | | W20_J160_run_ct6_body001.mrc (#33.1) coordinates: |
| 11658 | | Matrix rotation and translation |
| 11659 | | -0.32428809 -0.92759140 -0.18550319 265.85498427 |
| 11660 | | -0.58447784 0.04228657 0.81030704 122.87471915 |
| 11661 | | -0.74378954 0.37119543 -0.55586965 237.11232737 |
| 11662 | | Axis -0.55667228 0.70775295 0.43497325 |
| 11663 | | Axis point 213.01982007 0.00000000 38.79142480 |
| 11664 | | Rotation angle (degrees) 156.77096429 |
| 11665 | | Shift along axis 42.10836361 |
| 11666 | | |
| 11667 | | |
| 11668 | | > show #!51 models |
| 11669 | | |
| 11670 | | > show #!60 models |
| 11671 | | |
| 11672 | | > show #!61 models |
| 11673 | | |
| 11674 | | > show #!62 models |
| 11675 | | |
| 11676 | | > hide #!62 models |
| 11677 | | |
| 11678 | | > hide #!61 models |
| 11679 | | |
| 11680 | | > hide #!60 models |
| 11681 | | |
| 11682 | | > color #52 betav3 |
| 11683 | | |
| 11684 | | > color #53 betav3 |
| 11685 | | |
| 11686 | | > color #52 betav3 |
| 11687 | | |
| 11688 | | > color #52 betav1 |
| 11689 | | |
| 11690 | | > color #51 betav1 |
| 11691 | | |
| 11692 | | > show #!62 models |
| 11693 | | |
| 11694 | | > show #!61 models |
| 11695 | | |
| 11696 | | > show #!60 models |
| 11697 | | |
| 11698 | | > hide #!52 models |
| 11699 | | |
| 11700 | | > hide #!51 models |
| 11701 | | |
| 11702 | | > show #!53 models |
| 11703 | | |
| 11704 | | > color #53 betapv3 |
| 11705 | | |
| 11706 | | > hide #!53 models |
| 11707 | | |
| 11708 | | > show #50.1 models |
| 11709 | | |
| 11710 | | > hide #50.1 models |
| 11711 | | |
| 11712 | | > show #!50.2 models |
| 11713 | | |
| 11714 | | > hide #!50.2 models |
| 11715 | | |
| 11716 | | > show #!50.2 models |
| 11717 | | |
| 11718 | | > hide #!50.2 models |
| 11719 | | |
| 11720 | | > show #!50.3 models |
| 11721 | | |
| 11722 | | > hide #!50.3 models |
| 11723 | | |
| 11724 | | > show #!50.4 models |
| 11725 | | |
| 11726 | | > hide #!50.4 models |
| 11727 | | |
| 11728 | | > show #!50.5 models |
| 11729 | | |
| 11730 | | > hide #!50.5 models |
| 11731 | | |
| 11732 | | > show #50.6 models |
| 11733 | | |
| 11734 | | > show #!50.7 models |
| 11735 | | |
| 11736 | | > show #36 models |
| 11737 | | |
| 11738 | | > hide #36 models |
| 11739 | | |
| 11740 | | > show #36 models |
| 11741 | | |
| 11742 | | > ui tool show Matchmaker |
| 11743 | | |
| 11744 | | > matchmaker #50.6#!50.7 to #36 |
| 11745 | | |
| 11746 | | Parameters |
| 11747 | | --- |
| 11748 | | Chain pairing | bb |
| 11749 | | Alignment algorithm | Needleman-Wunsch |
| 11750 | | Similarity matrix | BLOSUM-62 |
| 11751 | | SS fraction | 0.3 |
| 11752 | | Gap open (HH/SS/other) | 18/18/6 |
| 11753 | | Gap extend | 1 |
| 11754 | | SS matrix | | | H | S | O |
| 11755 | | ---|---|---|--- |
| 11756 | | H | 6 | -9 | -6 |
| 11757 | | S | | 6 | -6 |
| 11758 | | O | | | 4 |
| 11759 | | Iteration cutoff | 2 |
| 11760 | | |
| 11761 | | Matchmaker CopBprime_O55029.pdb, chain A (#36) with |
| 11762 | | W20_C1_initial_model_no_wat_lig.pdb A5, chain A5 (#50.6), sequence alignment |
| 11763 | | score = 1599.3 |
| 11764 | | RMSD between 300 pruned atom pairs is 0.001 angstroms; (across all 300 pairs: |
| 11765 | | 0.001) |
| 11766 | | |
| 11767 | | Matchmaker CopBprime_O55029.pdb, chain A (#36) with |
| 11768 | | W20_C1_initial_model_no_wat_lig.pdb A6, chain A6 (#50.7), sequence alignment |
| 11769 | | score = 2777.6 |
| 11770 | | RMSD between 535 pruned atom pairs is 0.000 angstroms; (across all 535 pairs: |
| 11771 | | 0.000) |
| 11772 | | |
| 11773 | | |
| 11774 | | > hide #!60 models |
| 11775 | | |
| 11776 | | > hide #!61 models |
| 11777 | | |
| 11778 | | > hide #!62 models |
| 11779 | | |
| 11780 | | > hide #!33 models |
| 11781 | | |
| 11782 | | > show #!33 models |
| 11783 | | |
| 11784 | | > show #!29 models |
| 11785 | | |
| 11786 | | > hide #!33 models |
| 11787 | | |
| 11788 | | > hide #!29 models |
| 11789 | | |
| 11790 | | > show #!29 models |
| 11791 | | |
| 11792 | | > hide #!29 models |
| 11793 | | |
| 11794 | | > show #!7 models |
| 11795 | | |
| 11796 | | > hide #!7 models |
| 11797 | | |
| 11798 | | > show #!14 models |
| 11799 | | |
| 11800 | | > hide #!14 models |
| 11801 | | |
| 11802 | | > show #!15 models |
| 11803 | | |
| 11804 | | > hide #!15 models |
| 11805 | | |
| 11806 | | > show #!13 models |
| 11807 | | |
| 11808 | | > hide #!13 models |
| 11809 | | |
| 11810 | | > show #!29 models |
| 11811 | | |
| 11812 | | > hide #!29 models |
| 11813 | | |
| 11814 | | > show #!30 models |
| 11815 | | |
| 11816 | | > hide #!30 models |
| 11817 | | |
| 11818 | | > show #!31 models |
| 11819 | | |
| 11820 | | > hide #!31 models |
| 11821 | | |
| 11822 | | > show #!29 models |
| 11823 | | |
| 11824 | | > color #29 silver models |
| 11825 | | |
| 11826 | | > color #29 #c0c0c0bb models |
| 11827 | | |
| 11828 | | > fitmap #36 inMap #29.1 |
| 11829 | | |
| 11830 | | Fit molecule CopBprime_O55029.pdb (#36) to map W20_J140_run_body001.mrc |
| 11831 | | (#29.1) using 7214 atoms |
| 11832 | | average map value = 0.004094, steps = 196 |
| 11833 | | shifted from previous position = 6.85 |
| 11834 | | rotated from previous position = 8.52 degrees |
| 11835 | | atoms outside contour = 6338, contour level = 0.01322 |
| 11836 | | |
| 11837 | | Position of CopBprime_O55029.pdb (#36) relative to W20_J140_run_body001.mrc |
| 11838 | | (#29.1) coordinates: |
| 11839 | | Matrix rotation and translation |
| 11840 | | 0.34093560 0.24287995 0.90816972 145.36424309 |
| 11841 | | 0.84442936 0.34545023 -0.40939370 186.54646641 |
| 11842 | | -0.41316096 0.90646206 -0.08731868 180.11258285 |
| 11843 | | Axis 0.67156017 0.67435435 0.30700677 |
| 11844 | | Axis point 37.00439488 0.00000000 72.89161043 |
| 11845 | | Rotation angle (degrees) 101.56423540 |
| 11846 | | Shift along axis 278.71503775 |
| 11847 | | |
| 11848 | | |
| 11849 | | > fitmap #50.7 inMap #29.1 |
| 11850 | | |
| 11851 | | Fit molecule W20_C1_initial_model_no_wat_lig.pdb A6 (#50.7) to map |
| 11852 | | W20_J140_run_body001.mrc (#29.1) using 4264 atoms |
| 11853 | | average map value = 0.006635, steps = 664 |
| 11854 | | shifted from previous position = 6.45 |
| 11855 | | rotated from previous position = 16.7 degrees |
| 11856 | | atoms outside contour = 3295, contour level = 0.01322 |
| 11857 | | |
| 11858 | | Position of W20_C1_initial_model_no_wat_lig.pdb A6 (#50.7) relative to |
| 11859 | | W20_J140_run_body001.mrc (#29.1) coordinates: |
| 11860 | | Matrix rotation and translation |
| 11861 | | -0.98916440 0.14663035 -0.00730224 336.07347063 |
| 11862 | | -0.09208236 -0.65838724 -0.74702549 537.00653368 |
| 11863 | | -0.11434431 -0.73825862 0.66475529 165.63484820 |
| 11864 | | Axis 0.03349195 0.40893115 -0.91195044 |
| 11865 | | Axis point 189.25291418 293.62591224 0.00000000 |
| 11866 | | Rotation angle (degrees) 172.47953493 |
| 11867 | | Shift along axis 79.80368535 |
| 11868 | | |
| 11869 | | |
| 11870 | | > fitmap #50.6 inMap #29.4 |
| 11871 | | |
| 11872 | | Fit molecule W20_C1_initial_model_no_wat_lig.pdb A5 (#50.6) to map |
| 11873 | | W20_J140_run_body004.mrc (#29.4) using 2406 atoms |
| 11874 | | average map value = 0.01284, steps = 124 |
| 11875 | | shifted from previous position = 5.65 |
| 11876 | | rotated from previous position = 34.4 degrees |
| 11877 | | atoms outside contour = 1273, contour level = 0.012962 |
| 11878 | | |
| 11879 | | Position of W20_C1_initial_model_no_wat_lig.pdb A5 (#50.6) relative to |
| 11880 | | W20_J140_run_body004.mrc (#29.4) coordinates: |
| 11881 | | Matrix rotation and translation |
| 11882 | | -0.88088930 -0.47324067 0.00879232 419.53745111 |
| 11883 | | 0.15156939 -0.29963122 -0.94193835 456.11372933 |
| 11884 | | 0.44839799 -0.82841077 0.33567073 130.22033922 |
| 11885 | | Axis 0.14698922 -0.56917708 0.80896948 |
| 11886 | | Axis point 147.85180587 327.64605342 0.00000000 |
| 11887 | | Rotation angle (degrees) 157.28319935 |
| 11888 | | Shift along axis -92.59771834 |
| 11889 | | |
| 11890 | | |
| 11891 | | > hide #36 models |
| 11892 | | |
| 11893 | | > show #36 models |
| 11894 | | |
| 11895 | | > hide #36 models |
| 11896 | | |
| 11897 | | > hide #50.6 models |
| 11898 | | |
| 11899 | | > show #50.6 models |
| 11900 | | |
| 11901 | | > hide #50.6 models |
| 11902 | | |
| 11903 | | > hide #!29 models |
| 11904 | | |
| 11905 | | > show #50.6 models |
| 11906 | | |
| 11907 | | > show #36 models |
| 11908 | | |
| 11909 | | > hide #!50.7 models |
| 11910 | | |
| 11911 | | > hide #36 models |
| 11912 | | |
| 11913 | | > show #36 models |
| 11914 | | |
| 11915 | | > show #!53 models |
| 11916 | | |
| 11917 | | > hide #!53 models |
| 11918 | | |
| 11919 | | > close #53 |
| 11920 | | |
| 11921 | | > show #!63 models |
| 11922 | | |
| 11923 | | > hide #!63 models |
| 11924 | | |
| 11925 | | > close #63 |
| 11926 | | |
| 11927 | | > close #64 |
| 11928 | | |
| 11929 | | > close #65 |
| 11930 | | |
| 11931 | | > show #!50.7 models |
| 11932 | | |
| 11933 | | > combine #36 |
| 11934 | | |
| 11935 | | [Repeated 1 time(s)] |
| 11936 | | |
| 11937 | | > combine #50.7 |
| 11938 | | |
| 11939 | | > hide #!58 models |
| 11940 | | |
| 11941 | | > hide #!50.7 models |
| 11942 | | |
| 11943 | | > hide #57 models |
| 11944 | | |
| 11945 | | > hide #53 models |
| 11946 | | |
| 11947 | | > hide #36 models |
| 11948 | | |
| 11949 | | > show #!50.7 models |
| 11950 | | |
| 11951 | | > show #!58 models |
| 11952 | | |
| 11953 | | > hide #!58 models |
| 11954 | | |
| 11955 | | > show #!58 models |
| 11956 | | |
| 11957 | | > hide #!50.7 models |
| 11958 | | |
| 11959 | | > hide #!58 models |
| 11960 | | |
| 11961 | | > show #!58 models |
| 11962 | | |
| 11963 | | > hide #!58 models |
| 11964 | | |
| 11965 | | > combine #50.7 |
| 11966 | | |
| 11967 | | > hide #!59 models |
| 11968 | | |
| 11969 | | > show #!58 models |
| 11970 | | |
| 11971 | | > hide #50.6 models |
| 11972 | | |
| 11973 | | Drag select of 156 residues |
| 11974 | | |
| 11975 | | > select up |
| 11976 | | |
| 11977 | | 1543 atoms, 1574 bonds, 191 residues, 1 model selected |
| 11978 | | |
| 11979 | | > delete sel |
| 11980 | | |
| 11981 | | Drag select of 37 residues, 1 pseudobonds |
| 11982 | | |
| 11983 | | > select up |
| 11984 | | |
| 11985 | | 516 atoms, 519 bonds, 1 pseudobond, 65 residues, 2 models selected |
| 11986 | | |
| 11987 | | > delete sel |
| 11988 | | |
| 11989 | | Drag select of 7 residues, 1 pseudobonds |
| 11990 | | |
| 11991 | | > select clear |
| 11992 | | |
| 11993 | | Drag select of 6 residues, 1 pseudobonds |
| 11994 | | |
| 11995 | | > select up |
| 11996 | | |
| 11997 | | 54 atoms, 51 bonds, 1 pseudobond, 9 residues, 2 models selected |
| 11998 | | |
| 11999 | | > delete sel |
| 12000 | | |
| 12001 | | Drag select of 7 residues, 2 pseudobonds |
| 12002 | | |
| 12003 | | > select up |
| 12004 | | |
| 12005 | | 93 atoms, 93 bonds, 2 pseudobonds, 12 residues, 2 models selected |
| 12006 | | |
| 12007 | | > delete sel |
| 12008 | | |
| 12009 | | Drag select of 4 residues |
| 12010 | | |
| 12011 | | > delete sel |
| 12012 | | |
| 12013 | | > show #!29 models |
| 12014 | | |
| 12015 | | > fitmap #58 inMap #29.1 |
| 12016 | | |
| 12017 | | Fit molecule copy of W20_C1_initial_model_no_wat_lig.pdb A6 (#58) to map |
| 12018 | | W20_J140_run_body001.mrc (#29.1) using 2026 atoms |
| 12019 | | average map value = 0.01347, steps = 40 |
| 12020 | | shifted from previous position = 0.0954 |
| 12021 | | rotated from previous position = 0.431 degrees |
| 12022 | | atoms outside contour = 1055, contour level = 0.01322 |
| 12023 | | |
| 12024 | | Position of copy of W20_C1_initial_model_no_wat_lig.pdb A6 (#58) relative to |
| 12025 | | W20_J140_run_body001.mrc (#29.1) coordinates: |
| 12026 | | Matrix rotation and translation |
| 12027 | | -0.98886569 0.14811277 -0.01439634 337.90273979 |
| 12028 | | -0.08759046 -0.65752799 -0.74832136 536.28441247 |
| 12029 | | -0.12030195 -0.73872833 0.66318013 167.31953501 |
| 12030 | | Axis 0.03709874 0.40956482 -0.91152638 |
| 12031 | | Axis point 190.29117406 293.80476850 0.00000000 |
| 12032 | | Rotation angle (degrees) 172.57141027 |
| 12033 | | Shift along axis 79.66282889 |
| 12034 | | |
| 12035 | | |
| 12036 | | > show #!60 models |
| 12037 | | |
| 12038 | | > show #!61 models |
| 12039 | | |
| 12040 | | > show #!62 models |
| 12041 | | |
| 12042 | | > hide #!62 models |
| 12043 | | |
| 12044 | | > hide #!61 models |
| 12045 | | |
| 12046 | | > hide #!60 models |
| 12047 | | |
| 12048 | | > hide #!58 models |
| 12049 | | |
| 12050 | | > hide #!29 models |
| 12051 | | |
| 12052 | | > show #!59 models |
| 12053 | | |
| 12054 | | Drag select of 143 residues, 1 pseudobonds |
| 12055 | | |
| 12056 | | > select up |
| 12057 | | |
| 12058 | | 1146 atoms, 1169 bonds, 1 pseudobond, 145 residues, 2 models selected |
| 12059 | | |
| 12060 | | > delete sel |
| 12061 | | |
| 12062 | | > hide #!59 models |
| 12063 | | |
| 12064 | | > show #!58 models |
| 12065 | | |
| 12066 | | > hide #!58 models |
| 12067 | | |
| 12068 | | > show #!59 models |
| 12069 | | |
| 12070 | | Drag select of 51 residues |
| 12071 | | |
| 12072 | | > select up |
| 12073 | | |
| 12074 | | 444 atoms, 452 bonds, 55 residues, 1 model selected |
| 12075 | | |
| 12076 | | > delete sel |
| 12077 | | |
| 12078 | | Drag select of 27 residues |
| 12079 | | |
| 12080 | | > delete sel |
| 12081 | | |
| 12082 | | Drag select of 17 residues |
| 12083 | | |
| 12084 | | > select up |
| 12085 | | |
| 12086 | | 199 atoms, 201 bonds, 25 residues, 1 model selected |
| 12087 | | |
| 12088 | | > delete sel |
| 12089 | | |
| 12090 | | > select #59/A6:588 |
| 12091 | | |
| 12092 | | 8 atoms, 7 bonds, 1 residue, 1 model selected |
| 12093 | | |
| 12094 | | > select #59/A6:588 |
| 12095 | | |
| 12096 | | 8 atoms, 7 bonds, 1 residue, 1 model selected |
| 12097 | | |
| 12098 | | > show #!58 models |
| 12099 | | |
| 12100 | | > show #!29 models |
| 12101 | | |
| 12102 | | > hide #!29 models |
| 12103 | | |
| 12104 | | > hide #!58 models |
| 12105 | | |
| 12106 | | > delete sel |
| 12107 | | |
| 12108 | | > select #59/A6:587 |
| 12109 | | |
| 12110 | | 8 atoms, 7 bonds, 1 residue, 1 model selected |
| 12111 | | |
| 12112 | | > delete sel |
| 12113 | | |
| 12114 | | > show #!58 models |
| 12115 | | |
| 12116 | | > fitmap #59 inMap #29.4 |
| 12117 | | |
| 12118 | | Fit molecule copy of W20_C1_initial_model_no_wat_lig.pdb A6 (#59) to map |
| 12119 | | W20_J140_run_body004.mrc (#29.4) using 2238 atoms |
| 12120 | | average map value = 0.01488, steps = 128 |
| 12121 | | shifted from previous position = 9.04 |
| 12122 | | rotated from previous position = 24.7 degrees |
| 12123 | | atoms outside contour = 1046, contour level = 0.012962 |
| 12124 | | |
| 12125 | | Position of copy of W20_C1_initial_model_no_wat_lig.pdb A6 (#59) relative to |
| 12126 | | W20_J140_run_body004.mrc (#29.4) coordinates: |
| 12127 | | Matrix rotation and translation |
| 12128 | | -0.85413410 0.51616797 -0.06344729 245.49771496 |
| 12129 | | -0.20546679 -0.44701353 -0.87061031 558.08387697 |
| 12130 | | -0.47774295 -0.73058164 0.48786487 300.68201418 |
| 12131 | | Axis 0.16594922 0.49098549 -0.85521582 |
| 12132 | | Axis point 220.46254144 334.83017754 0.00000000 |
| 12133 | | Rotation angle (degrees) 155.04526429 |
| 12134 | | Shift along axis 57.60322791 |
| 12135 | | |
| 12136 | | |
| 12137 | | > show #!29 models |
| 12138 | | |
| 12139 | | > show #50.6 models |
| 12140 | | |
| 12141 | | > hide #!59 models |
| 12142 | | |
| 12143 | | > hide #!58 models |
| 12144 | | |
| 12145 | | > show #53 models |
| 12146 | | |
| 12147 | | > select #29.2 |
| 12148 | | |
| 12149 | | 2 models selected |
| 12150 | | |
| 12151 | | > select #29.2 |
| 12152 | | |
| 12153 | | 2 models selected |
| 12154 | | |
| 12155 | | > select clear |
| 12156 | | |
| 12157 | | [Repeated 1 time(s)] |
| 12158 | | |
| 12159 | | > select #53/A:876 |
| 12160 | | |
| 12161 | | 8 atoms, 7 bonds, 1 residue, 1 model selected |
| 12162 | | |
| 12163 | | > select up |
| 12164 | | |
| 12165 | | 519 atoms, 525 bonds, 67 residues, 1 model selected |
| 12166 | | |
| 12167 | | > delete sel |
| 12168 | | |
| 12169 | | > show #!58 models |
| 12170 | | |
| 12171 | | > hide #!58 models |
| 12172 | | |
| 12173 | | > select clear |
| 12174 | | |
| 12175 | | > select #53/A:640 |
| 12176 | | |
| 12177 | | 8 atoms, 7 bonds, 1 residue, 1 model selected |
| 12178 | | |
| 12179 | | > hide #!29 models |
| 12180 | | |
| 12181 | | Drag select of 184 residues |
| 12182 | | |
| 12183 | | > select up |
| 12184 | | |
| 12185 | | 1682 atoms, 1717 bonds, 208 residues, 1 model selected |
| 12186 | | |
| 12187 | | > delete sel |
| 12188 | | |
| 12189 | | > hide #50.6 models |
| 12190 | | |
| 12191 | | Drag select of 419 residues, 4 pseudobonds |
| 12192 | | |
| 12193 | | > select up |
| 12194 | | |
| 12195 | | 3440 atoms, 3516 bonds, 4 pseudobonds, 432 residues, 2 models selected |
| 12196 | | |
| 12197 | | > delete sel |
| 12198 | | |
| 12199 | | > fitmap #53 inMap #29.1 |
| 12200 | | |
| 12201 | | Fit molecule copy of CopBprime_O55029.pdb (#53) to map |
| 12202 | | W20_J140_run_body001.mrc (#29.1) using 1573 atoms |
| 12203 | | average map value = 0.0152, steps = 92 |
| 12204 | | shifted from previous position = 4.75 |
| 12205 | | rotated from previous position = 20.8 degrees |
| 12206 | | atoms outside contour = 682, contour level = 0.01322 |
| 12207 | | |
| 12208 | | Position of copy of CopBprime_O55029.pdb (#53) relative to |
| 12209 | | W20_J140_run_body001.mrc (#29.1) coordinates: |
| 12210 | | Matrix rotation and translation |
| 12211 | | 0.22399917 0.35238232 0.90865344 142.70226714 |
| 12212 | | 0.67056970 0.62083889 -0.40607308 181.66322382 |
| 12213 | | -0.70722037 0.70027550 -0.09722951 169.81132058 |
| 12214 | | Axis 0.55763236 0.81444812 0.16037584 |
| 12215 | | Axis point 78.89411363 0.00000000 66.67498198 |
| 12216 | | Rotation angle (degrees) 97.24981271 |
| 12217 | | Shift along axis 254.76430584 |
| 12218 | | |
| 12219 | | |
| 12220 | | > show #!29 models |
| 12221 | | |
| 12222 | | > hide #!53 models |
| 12223 | | |
| 12224 | | > show #!53 models |
| 12225 | | |
| 12226 | | > show #!58 models |
| 12227 | | |
| 12228 | | > hide #!58 models |
| 12229 | | |
| 12230 | | > hide #!53 models |
| 12231 | | |
| 12232 | | > show #!53 models |
| 12233 | | |
| 12234 | | > hide #!53 models |
| 12235 | | |
| 12236 | | > show #!53 models |
| 12237 | | |
| 12238 | | > hide #!53 models |
| 12239 | | |
| 12240 | | > show #!53 models |
| 12241 | | |
| 12242 | | > show #57 models |
| 12243 | | |
| 12244 | | > hide #57 models |
| 12245 | | |
| 12246 | | > hide #!29 models |
| 12247 | | |
| 12248 | | > hide #!53 models |
| 12249 | | |
| 12250 | | > show #57 models |
| 12251 | | |
| 12252 | | Drag select of 6 residues |
| 12253 | | |
| 12254 | | > select up |
| 12255 | | |
| 12256 | | 519 atoms, 525 bonds, 67 residues, 1 model selected |
| 12257 | | |
| 12258 | | > delete sel |
| 12259 | | |
| 12260 | | Drag select of 159 residues |
| 12261 | | |
| 12262 | | > select up |
| 12263 | | |
| 12264 | | 1356 atoms, 1383 bonds, 171 residues, 1 model selected |
| 12265 | | |
| 12266 | | > delete sel |
| 12267 | | |
| 12268 | | Drag select of 13 residues, 1 pseudobonds |
| 12269 | | |
| 12270 | | > select up |
| 12271 | | |
| 12272 | | 109 atoms, 108 bonds, 1 pseudobond, 14 residues, 2 models selected |
| 12273 | | |
| 12274 | | > delete sel |
| 12275 | | |
| 12276 | | Drag select of 5 residues |
| 12277 | | |
| 12278 | | > select clear |
| 12279 | | |
| 12280 | | Drag select of 8 residues |
| 12281 | | |
| 12282 | | > select up |
| 12283 | | |
| 12284 | | 108 atoms, 110 bonds, 13 residues, 1 model selected |
| 12285 | | |
| 12286 | | > delete sel |
| 12287 | | |
| 12288 | | > fitmap #57 inMap #29.4 |
| 12289 | | |
| 12290 | | Fit molecule copy of CopBprime_O55029.pdb (#57) to map |
| 12291 | | W20_J140_run_body004.mrc (#29.4) using 5122 atoms |
| 12292 | | average map value = 0.01184, steps = 144 |
| 12293 | | shifted from previous position = 8.94 |
| 12294 | | rotated from previous position = 13.8 degrees |
| 12295 | | atoms outside contour = 3092, contour level = 0.012962 |
| 12296 | | |
| 12297 | | Position of copy of CopBprime_O55029.pdb (#57) relative to |
| 12298 | | W20_J140_run_body004.mrc (#29.4) coordinates: |
| 12299 | | Matrix rotation and translation |
| 12300 | | 0.19336342 0.06063554 0.97925171 138.80893393 |
| 12301 | | 0.87031534 0.45017771 -0.19972793 186.94884028 |
| 12302 | | -0.45294790 0.89087786 0.03427586 177.78973972 |
| 12303 | | Axis 0.55251909 0.72557622 0.41019729 |
| 12304 | | Axis point 33.82238390 0.00000000 47.06660425 |
| 12305 | | Rotation angle (degrees) 99.27025680 |
| 12306 | | Shift along axis 285.26908646 |
| 12307 | | |
| 12308 | | |
| 12309 | | > show #!29 models |
| 12310 | | |
| 12311 | | > ui tool show "Build Structure" |
| 12312 | | |
| 12313 | | > toolshed show |
| 12314 | | |
| 12315 | | > show #!53 models |
| 12316 | | |
| 12317 | | > hide #!53 models |
| 12318 | | |
| 12319 | | > fitmap #57 inMap #29.4 |
| 12320 | | |
| 12321 | | Fit molecule copy of CopBprime_O55029.pdb (#57) to map |
| 12322 | | W20_J140_run_body004.mrc (#29.4) using 5122 atoms |
| 12323 | | average map value = 0.01184, steps = 104 |
| 12324 | | shifted from previous position = 0.0162 |
| 12325 | | rotated from previous position = 0.0152 degrees |
| 12326 | | atoms outside contour = 3088, contour level = 0.012962 |
| 12327 | | |
| 12328 | | Position of copy of CopBprime_O55029.pdb (#57) relative to |
| 12329 | | W20_J140_run_body004.mrc (#29.4) coordinates: |
| 12330 | | Matrix rotation and translation |
| 12331 | | 0.19340630 0.06050533 0.97925130 138.80565926 |
| 12332 | | 0.87021654 0.45038133 -0.19969933 186.94266371 |
| 12333 | | -0.45311938 0.89078379 0.03445385 177.80765427 |
| 12334 | | Axis 0.55243756 0.72563743 0.41019882 |
| 12335 | | Axis point 33.85424725 0.00000000 47.07180428 |
| 12336 | | Rotation angle (degrees) 99.25793540 |
| 12337 | | Shift along axis 285.27054234 |
| 12338 | | |
| 12339 | | |
| 12340 | | > combine #57 |
| 12341 | | |
| 12342 | | > hide #!29 models |
| 12343 | | |
| 12344 | | > hide #!63 models |
| 12345 | | |
| 12346 | | Drag select of 2 residues |
| 12347 | | |
| 12348 | | > select up |
| 12349 | | |
| 12350 | | 50 atoms, 50 bonds, 6 residues, 1 model selected |
| 12351 | | Drag select of 293 residues |
| 12352 | | |
| 12353 | | > select up |
| 12354 | | |
| 12355 | | 2400 atoms, 2461 bonds, 299 residues, 1 model selected |
| 12356 | | |
| 12357 | | > delete sel |
| 12358 | | |
| 12359 | | > fitmap #57 inMap #29.4 |
| 12360 | | |
| 12361 | | Fit molecule copy of CopBprime_O55029.pdb (#57) to map |
| 12362 | | W20_J140_run_body004.mrc (#29.4) using 2722 atoms |
| 12363 | | average map value = 0.01488, steps = 60 |
| 12364 | | shifted from previous position = 0.588 |
| 12365 | | rotated from previous position = 4.72 degrees |
| 12366 | | atoms outside contour = 1280, contour level = 0.012962 |
| 12367 | | |
| 12368 | | Position of copy of CopBprime_O55029.pdb (#57) relative to |
| 12369 | | W20_J140_run_body004.mrc (#29.4) coordinates: |
| 12370 | | Matrix rotation and translation |
| 12371 | | 0.24483069 0.00098871 0.96956534 140.64125601 |
| 12372 | | 0.85587662 0.46963714 -0.21660139 186.15669395 |
| 12373 | | -0.45555806 0.88285898 0.11413537 178.37372881 |
| 12374 | | Axis 0.55176004 0.71519280 0.42902227 |
| 12375 | | Axis point 33.88188669 0.00000000 44.81013344 |
| 12376 | | Rotation angle (degrees) 94.91618649 |
| 12377 | | Shift along axis 287.26445575 |
| 12378 | | |
| 12379 | | |
| 12380 | | > show #!29 models |
| 12381 | | |
| 12382 | | > select add #57 |
| 12383 | | |
| 12384 | | 2722 atoms, 2779 bonds, 341 residues, 1 model selected |
| 12385 | | |
| 12386 | | > select clear |
| 12387 | | |
| 12388 | | > select add #57 |
| 12389 | | |
| 12390 | | 2722 atoms, 2779 bonds, 341 residues, 1 model selected |
| 12391 | | |
| 12392 | | > ui mousemode right "translate selected models" |
| 12393 | | |
| 12394 | | > view matrix models |
| 12395 | | > #57,0.50755,-0.10762,0.85488,130.21,0.68026,0.65898,-0.32092,190.13,-0.52881,0.74442,0.40768,177.92 |
| 12396 | | |
| 12397 | | > fitmap #57 inMap #29.4 |
| 12398 | | |
| 12399 | | Fit molecule copy of CopBprime_O55029.pdb (#57) to map |
| 12400 | | W20_J140_run_body004.mrc (#29.4) using 2722 atoms |
| 12401 | | average map value = 0.01488, steps = 64 |
| 12402 | | shifted from previous position = 2.72 |
| 12403 | | rotated from previous position = 0.042 degrees |
| 12404 | | atoms outside contour = 1279, contour level = 0.012962 |
| 12405 | | |
| 12406 | | Position of copy of CopBprime_O55029.pdb (#57) relative to |
| 12407 | | W20_J140_run_body004.mrc (#29.4) coordinates: |
| 12408 | | Matrix rotation and translation |
| 12409 | | 0.24476466 0.00100564 0.96958200 140.62777342 |
| 12410 | | 0.85622153 0.46899298 -0.21663398 186.17961813 |
| 12411 | | -0.45494501 0.88320132 0.11393185 178.36605751 |
| 12412 | | Axis 0.55197003 0.71492178 0.42920384 |
| 12413 | | Axis point 33.76576678 0.00000000 44.80708945 |
| 12414 | | Rotation angle (degrees) 94.94245961 |
| 12415 | | Shift along axis 287.28157686 |
| 12416 | | |
| 12417 | | |
| 12418 | | > select subtract #57 |
| 12419 | | |
| 12420 | | Nothing selected |
| 12421 | | |
| 12422 | | > hide #!57 models |
| 12423 | | |
| 12424 | | > show #!63 models |
| 12425 | | |
| 12426 | | > hide #!29 models |
| 12427 | | |
| 12428 | | > ui mousemode right select |
| 12429 | | |
| 12430 | | Drag select of 285 residues |
| 12431 | | |
| 12432 | | > select up |
| 12433 | | |
| 12434 | | 2450 atoms, 2494 bonds, 306 residues, 1 model selected |
| 12435 | | |
| 12436 | | > select up |
| 12437 | | |
| 12438 | | 5122 atoms, 5241 bonds, 640 residues, 1 model selected |
| 12439 | | |
| 12440 | | > select clear |
| 12441 | | |
| 12442 | | Drag select of 338 residues |
| 12443 | | |
| 12444 | | > select up |
| 12445 | | |
| 12446 | | 2722 atoms, 2779 bonds, 341 residues, 1 model selected |
| 12447 | | |
| 12448 | | > select clear |
| 12449 | | |
| 12450 | | Drag select of 321 residues |
| 12451 | | |
| 12452 | | > select up |
| 12453 | | |
| 12454 | | 2643 atoms, 2694 bonds, 331 residues, 1 model selected |
| 12455 | | |
| 12456 | | > select clear |
| 12457 | | |
| 12458 | | Drag select of 193 residues |
| 12459 | | |
| 12460 | | > select up |
| 12461 | | |
| 12462 | | 2186 atoms, 2221 bonds, 272 residues, 1 model selected |
| 12463 | | |
| 12464 | | > delete sel |
| 12465 | | |
| 12466 | | > show #!57 models |
| 12467 | | |
| 12468 | | > hide #!63 models |
| 12469 | | |
| 12470 | | > hide #!57 models |
| 12471 | | |
| 12472 | | > show #!63 models |
| 12473 | | |
| 12474 | | > select #63/A:10 |
| 12475 | | |
| 12476 | | 9 atoms, 8 bonds, 1 residue, 1 model selected |
| 12477 | | |
| 12478 | | > select #63/A:577 |
| 12479 | | |
| 12480 | | 8 atoms, 7 bonds, 1 residue, 1 model selected |
| 12481 | | |
| 12482 | | > select up |
| 12483 | | |
| 12484 | | 219 atoms, 213 bonds, 27 residues, 1 model selected |
| 12485 | | |
| 12486 | | > delete sel |
| 12487 | | |
| 12488 | | > select clear |
| 12489 | | |
| 12490 | | > select #63/A:430 |
| 12491 | | |
| 12492 | | 5 atoms, 4 bonds, 1 residue, 1 model selected |
| 12493 | | Drag select of 14 residues, 1 pseudobonds |
| 12494 | | |
| 12495 | | > select clear |
| 12496 | | |
| 12497 | | Drag select of 16 residues, 3 pseudobonds |
| 12498 | | |
| 12499 | | > select up |
| 12500 | | |
| 12501 | | 125 atoms, 125 bonds, 3 pseudobonds, 17 residues, 2 models selected |
| 12502 | | |
| 12503 | | > delete sel |
| 12504 | | |
| 12505 | | Drag select of 3 residues |
| 12506 | | |
| 12507 | | > delete sel |
| 12508 | | |
| 12509 | | Drag select of 3 residues |
| 12510 | | |
| 12511 | | > select up |
| 12512 | | |
| 12513 | | 42 atoms, 41 bonds, 6 residues, 1 model selected |
| 12514 | | |
| 12515 | | > delete sel |
| 12516 | | |
| 12517 | | > select clear |
| 12518 | | |
| 12519 | | > select #63/A:353 |
| 12520 | | |
| 12521 | | 8 atoms, 7 bonds, 1 residue, 1 model selected |
| 12522 | | |
| 12523 | | > select up |
| 12524 | | |
| 12525 | | 124 atoms, 124 bonds, 16 residues, 1 model selected |
| 12526 | | |
| 12527 | | > select clear |
| 12528 | | |
| 12529 | | [Repeated 1 time(s)]Drag select of 1 residues |
| 12530 | | |
| 12531 | | > select down |
| 12532 | | |
| 12533 | | 4 atoms, 1 residue, 1 model selected |
| 12534 | | |
| 12535 | | > select up |
| 12536 | | |
| 12537 | | 124 atoms, 124 bonds, 16 residues, 1 model selected |
| 12538 | | |
| 12539 | | > select clear |
| 12540 | | |
| 12541 | | > select #63/A:373 |
| 12542 | | |
| 12543 | | 4 atoms, 3 bonds, 1 residue, 1 model selected |
| 12544 | | |
| 12545 | | > delete sel |
| 12546 | | |
| 12547 | | > select clear |
| 12548 | | |
| 12549 | | > select #63/A:354 |
| 12550 | | |
| 12551 | | 12 atoms, 12 bonds, 1 residue, 1 model selected |
| 12552 | | |
| 12553 | | > delete sel |
| 12554 | | |
| 12555 | | > select clear |
| 12556 | | |
| 12557 | | > select #63/A:356 |
| 12558 | | |
| 12559 | | 9 atoms, 8 bonds, 1 residue, 1 model selected |
| 12560 | | |
| 12561 | | > \ |
| 12562 | | |
| 12563 | | Unknown command: \ |
| 12564 | | |
| 12565 | | > select clear |
| 12566 | | |
| 12567 | | > select #63/A:355 |
| 12568 | | |
| 12569 | | 7 atoms, 7 bonds, 1 residue, 1 model selected |
| 12570 | | |
| 12571 | | > \ |
| 12572 | | |
| 12573 | | Unknown command: \ |
| 12574 | | |
| 12575 | | > delete sel |
| 12576 | | |
| 12577 | | > select clear |
| 12578 | | |
| 12579 | | [Repeated 1 time(s)]Drag select of 3 residues, 2 pseudobonds |
| 12580 | | |
| 12581 | | > delete sel |
| 12582 | | |
| 12583 | | > select #63/A:320 |
| 12584 | | |
| 12585 | | 6 atoms, 5 bonds, 1 residue, 1 model selected |
| 12586 | | Drag select of 1 residues, 2 pseudobonds |
| 12587 | | |
| 12588 | | > select up |
| 12589 | | |
| 12590 | | 78 atoms, 76 bonds, 2 pseudobonds, 10 residues, 2 models selected |
| 12591 | | |
| 12592 | | > close #63 |
| 12593 | | |
| 12594 | | > combine #36 |
| 12595 | | |
| 12596 | | Drag select of 280 residues |
| 12597 | | |
| 12598 | | > select up |
| 12599 | | |
| 12600 | | 3003 atoms, 3050 bonds, 379 residues, 1 model selected |
| 12601 | | |
| 12602 | | > delete sel |
| 12603 | | |
| 12604 | | Drag select of 41 residues, 2 pseudobonds |
| 12605 | | |
| 12606 | | > delete sel |
| 12607 | | |
| 12608 | | Drag select of 122 residues, 3 pseudobonds |
| 12609 | | |
| 12610 | | > select up |
| 12611 | | |
| 12612 | | 1221 atoms, 1239 bonds, 3 pseudobonds, 152 residues, 2 models selected |
| 12613 | | |
| 12614 | | > delete sel |
| 12615 | | |
| 12616 | | Drag select of 9 residues, 2 pseudobonds |
| 12617 | | |
| 12618 | | > select up |
| 12619 | | |
| 12620 | | 91 atoms, 90 bonds, 2 pseudobonds, 12 residues, 2 models selected |
| 12621 | | |
| 12622 | | > delete sel |
| 12623 | | |
| 12624 | | > select #63/A:373 |
| 12625 | | |
| 12626 | | 4 atoms, 3 bonds, 1 residue, 1 model selected |
| 12627 | | |
| 12628 | | > delete sel |
| 12629 | | |
| 12630 | | > select #63/A:356 |
| 12631 | | |
| 12632 | | 9 atoms, 8 bonds, 1 residue, 1 model selected |
| 12633 | | |
| 12634 | | > delete sel |
| 12635 | | |
| 12636 | | > close #63 |
| 12637 | | |
| 12638 | | > show #!53 models |
| 12639 | | |
| 12640 | | > hide #!53 models |
| 12641 | | |
| 12642 | | > show #!57 models |
| 12643 | | |
| 12644 | | > hide #!57 models |
| 12645 | | |
| 12646 | | > show #50.1 models |
| 12647 | | |
| 12648 | | > hide #50.1 models |
| 12649 | | |
| 12650 | | > show #!50.2 models |
| 12651 | | |
| 12652 | | > hide #!50.2 models |
| 12653 | | |
| 12654 | | > combine #36 |
| 12655 | | |
| 12656 | | Drag select of 289 residues |
| 12657 | | |
| 12658 | | > select up |
| 12659 | | |
| 12660 | | 2306 atoms, 2348 bonds, 291 residues, 1 model selected |
| 12661 | | |
| 12662 | | > delete sel |
| 12663 | | |
| 12664 | | Drag select of 218 residues |
| 12665 | | |
| 12666 | | > select up |
| 12667 | | |
| 12668 | | 1888 atoms, 1925 bonds, 237 residues, 1 model selected |
| 12669 | | |
| 12670 | | > delete sel |
| 12671 | | |
| 12672 | | Drag select of 15 residues, 1 pseudobonds |
| 12673 | | |
| 12674 | | > select up |
| 12675 | | |
| 12676 | | 152 atoms, 153 bonds, 1 pseudobond, 18 residues, 2 models selected |
| 12677 | | |
| 12678 | | > delete sel |
| 12679 | | |
| 12680 | | Drag select of 4 residues |
| 12681 | | |
| 12682 | | > select up |
| 12683 | | |
| 12684 | | 42 atoms, 41 bonds, 5 residues, 1 model selected |
| 12685 | | |
| 12686 | | > delete sel |
| 12687 | | |
| 12688 | | Drag select of 29 residues, 2 pseudobonds |
| 12689 | | |
| 12690 | | > select up |
| 12691 | | |
| 12692 | | 302 atoms, 302 bonds, 2 pseudobonds, 39 residues, 2 models selected |
| 12693 | | |
| 12694 | | > delete sel |
| 12695 | | |
| 12696 | | > select clear |
| 12697 | | |
| 12698 | | [Repeated 1 time(s)] |
| 12699 | | |
| 12700 | | > select #63/A:305 |
| 12701 | | |
| 12702 | | 5 atoms, 4 bonds, 1 residue, 1 model selected |
| 12703 | | |
| 12704 | | > delete sel |
| 12705 | | |
| 12706 | | > select clear |
| 12707 | | |
| 12708 | | Drag select of 8 residues, 1 pseudobonds |
| 12709 | | |
| 12710 | | > select up |
| 12711 | | |
| 12712 | | 119 atoms, 119 bonds, 1 pseudobond, 15 residues, 2 models selected |
| 12713 | | |
| 12714 | | > show #!57 models |
| 12715 | | |
| 12716 | | > delete sel |
| 12717 | | |
| 12718 | | > fitmap #63 inMap #29.4 |
| 12719 | | |
| 12720 | | Fit molecule copy of CopBprime_O55029.pdb (#63) to map |
| 12721 | | W20_J140_run_body004.mrc (#29.4) using 2400 atoms |
| 12722 | | average map value = 0.01282, steps = 148 |
| 12723 | | shifted from previous position = 15.9 |
| 12724 | | rotated from previous position = 27.5 degrees |
| 12725 | | atoms outside contour = 1275, contour level = 0.012962 |
| 12726 | | |
| 12727 | | Position of copy of CopBprime_O55029.pdb (#63) relative to |
| 12728 | | W20_J140_run_body004.mrc (#29.4) coordinates: |
| 12729 | | Matrix rotation and translation |
| 12730 | | 0.53194142 0.55442248 0.64004222 123.33487031 |
| 12731 | | 0.70035644 0.13679328 -0.70056295 183.98600531 |
| 12732 | | -0.47596132 0.82091614 -0.31552735 169.31259792 |
| 12733 | | Axis 0.80394040 0.58968956 0.07711063 |
| 12734 | | Axis point 0.00000000 -20.96531712 102.68367487 |
| 12735 | | Rotation angle (degrees) 108.86844509 |
| 12736 | | Shift along axis 220.70431310 |
| 12737 | | |
| 12738 | | |
| 12739 | | > show #!29 models |
| 12740 | | |
| 12741 | | > show #!53 models |
| 12742 | | |
| 12743 | | > show #!58 models |
| 12744 | | |
| 12745 | | > hide #!58 models |
| 12746 | | |
| 12747 | | > show #!58 models |
| 12748 | | |
| 12749 | | > hide #!53 models |
| 12750 | | |
| 12751 | | > show #!53 models |
| 12752 | | |
| 12753 | | > hide #!57 models |
| 12754 | | |
| 12755 | | > show #!57 models |
| 12756 | | |
| 12757 | | > hide #!57 models |
| 12758 | | |
| 12759 | | > show #!59 models |
| 12760 | | |
| 12761 | | > hide #!53 models |
| 12762 | | |
| 12763 | | > hide #!63 models |
| 12764 | | |
| 12765 | | > show #!63 models |
| 12766 | | |
| 12767 | | > hide #!63 models |
| 12768 | | |
| 12769 | | > show #!57 models |
| 12770 | | |
| 12771 | | > hide #!57 models |
| 12772 | | |
| 12773 | | > show #!57 models |
| 12774 | | |
| 12775 | | > hide #!57 models |
| 12776 | | |
| 12777 | | > show #!53 models |
| 12778 | | |
| 12779 | | > hide #!53 models |
| 12780 | | |
| 12781 | | > show #!53 models |
| 12782 | | |
| 12783 | | > hide #!53 models |
| 12784 | | |
| 12785 | | > show #!63 models |
| 12786 | | |
| 12787 | | > hide #!63 models |
| 12788 | | |
| 12789 | | > show #50.6 models |
| 12790 | | |
| 12791 | | > show #!63 models |
| 12792 | | |
| 12793 | | > hide #!63 models |
| 12794 | | |
| 12795 | | > show #!53 models |
| 12796 | | |
| 12797 | | > hide #!53 models |
| 12798 | | |
| 12799 | | > show #!53 models |
| 12800 | | |
| 12801 | | > hide #!53 models |
| 12802 | | |
| 12803 | | > show #!57 models |
| 12804 | | |
| 12805 | | > hide #!57 models |
| 12806 | | |
| 12807 | | > show #!57 models |
| 12808 | | |
| 12809 | | > hide #!57 models |
| 12810 | | |
| 12811 | | > combine #57 |
| 12812 | | |
| 12813 | | > hide #!58 models |
| 12814 | | |
| 12815 | | > hide #!59 models |
| 12816 | | |
| 12817 | | > hide #50.6 models |
| 12818 | | |
| 12819 | | > show #!63 models |
| 12820 | | |
| 12821 | | > hide #!63 models |
| 12822 | | |
| 12823 | | > show #!63 models |
| 12824 | | |
| 12825 | | > hide #!63 models |
| 12826 | | |
| 12827 | | > show #!63 models |
| 12828 | | |
| 12829 | | > hide #!29 models |
| 12830 | | |
| 12831 | | > show #50.6 models |
| 12832 | | |
| 12833 | | > hide #50.6 models |
| 12834 | | |
| 12835 | | > hide #!63 models |
| 12836 | | |
| 12837 | | > show #!57 models |
| 12838 | | |
| 12839 | | > hide #!57 models |
| 12840 | | |
| 12841 | | > show #!57 models |
| 12842 | | |
| 12843 | | > hide #!57 models |
| 12844 | | |
| 12845 | | Drag select of 26 residues |
| 12846 | | |
| 12847 | | > select up |
| 12848 | | |
| 12849 | | 229 atoms, 232 bonds, 28 residues, 1 model selected |
| 12850 | | |
| 12851 | | > delete sel |
| 12852 | | |
| 12853 | | Drag select of 9 residues |
| 12854 | | |
| 12855 | | > select up |
| 12856 | | |
| 12857 | | 76 atoms, 77 bonds, 10 residues, 1 model selected |
| 12858 | | |
| 12859 | | > delete sel |
| 12860 | | |
| 12861 | | Drag select of 6 residues |
| 12862 | | |
| 12863 | | > select clear |
| 12864 | | |
| 12865 | | Drag select of 10 residues |
| 12866 | | |
| 12867 | | > select up |
| 12868 | | |
| 12869 | | 115 atoms, 115 bonds, 14 residues, 1 model selected |
| 12870 | | |
| 12871 | | > combine #57 |
| 12872 | | |
| 12873 | | > select add #64 |
| 12874 | | |
| 12875 | | 2417 atoms, 2468 bonds, 303 residues, 1 model selected |
| 12876 | | |
| 12877 | | > close #64 |
| 12878 | | |
| 12879 | | > hide #!65 models |
| 12880 | | |
| 12881 | | > show #!65 models |
| 12882 | | |
| 12883 | | > show #!63 models |
| 12884 | | |
| 12885 | | > hide #!63 models |
| 12886 | | |
| 12887 | | > show #!63 models |
| 12888 | | |
| 12889 | | > hide #!63 models |
| 12890 | | |
| 12891 | | > show #!57 models |
| 12892 | | |
| 12893 | | > hide #!57 models |
| 12894 | | |
| 12895 | | > show #!57 models |
| 12896 | | |
| 12897 | | > hide #!65 models |
| 12898 | | |
| 12899 | | Drag select of 31 residues |
| 12900 | | |
| 12901 | | > select up |
| 12902 | | |
| 12903 | | 305 atoms, 310 bonds, 38 residues, 1 model selected |
| 12904 | | |
| 12905 | | > delete sel |
| 12906 | | |
| 12907 | | Drag select of 13 residues |
| 12908 | | |
| 12909 | | > select up |
| 12910 | | |
| 12911 | | 115 atoms, 115 bonds, 14 residues, 1 model selected |
| 12912 | | |
| 12913 | | > delete sel |
| 12914 | | |
| 12915 | | > hide #!57 models |
| 12916 | | |
| 12917 | | > show #!53 models |
| 12918 | | |
| 12919 | | > show #!57 models |
| 12920 | | |
| 12921 | | > fitmap #57 inMap #29.4 |
| 12922 | | |
| 12923 | | Fit molecule copy of CopBprime_O55029.pdb (#57) to map |
| 12924 | | W20_J140_run_body004.mrc (#29.4) using 2302 atoms |
| 12925 | | average map value = 0.01478, steps = 84 |
| 12926 | | shifted from previous position = 0.608 |
| 12927 | | rotated from previous position = 2.11 degrees |
| 12928 | | atoms outside contour = 1083, contour level = 0.012962 |
| 12929 | | |
| 12930 | | Position of copy of CopBprime_O55029.pdb (#57) relative to |
| 12931 | | W20_J140_run_body004.mrc (#29.4) coordinates: |
| 12932 | | Matrix rotation and translation |
| 12933 | | 0.26430656 -0.02399441 0.96414019 140.91312010 |
| 12934 | | 0.84653335 0.48474763 -0.22000233 186.26213690 |
| 12935 | | -0.46208585 0.87432489 0.14843404 179.06445534 |
| 12936 | | Axis 0.54788377 0.71405160 0.43583676 |
| 12937 | | Axis point 34.54414646 0.00000000 44.00500794 |
| 12938 | | Rotation angle (degrees) 92.93803311 |
| 12939 | | Shift along axis 288.24766132 |
| 12940 | | |
| 12941 | | |
| 12942 | | > show #!63 models |
| 12943 | | |
| 12944 | | > show #!65 models |
| 12945 | | |
| 12946 | | > hide #!65 models |
| 12947 | | |
| 12948 | | > show #!65 models |
| 12949 | | |
| 12950 | | > hide #!65 models |
| 12951 | | |
| 12952 | | > show #!65 models |
| 12953 | | |
| 12954 | | > hide #!57 models |
| 12955 | | |
| 12956 | | > hide #!63 models |
| 12957 | | |
| 12958 | | Drag select of 243 residues |
| 12959 | | |
| 12960 | | > select up |
| 12961 | | |
| 12962 | | 2119 atoms, 2164 bonds, 266 residues, 1 model selected |
| 12963 | | |
| 12964 | | > delete sel |
| 12965 | | |
| 12966 | | > hide #!65 models |
| 12967 | | |
| 12968 | | > show #!57 models |
| 12969 | | |
| 12970 | | > show #!63 models |
| 12971 | | |
| 12972 | | > hide #!63 models |
| 12973 | | |
| 12974 | | > hide #!57 models |
| 12975 | | |
| 12976 | | > show #!65 models |
| 12977 | | |
| 12978 | | Drag select of 17 residues, 1 pseudobonds |
| 12979 | | |
| 12980 | | > select up |
| 12981 | | |
| 12982 | | 159 atoms, 159 bonds, 1 pseudobond, 19 residues, 2 models selected |
| 12983 | | Drag select of 22 residues, 2 pseudobonds |
| 12984 | | |
| 12985 | | > delete sel |
| 12986 | | |
| 12987 | | > show #!57 models |
| 12988 | | |
| 12989 | | > select #57/A:547 |
| 12990 | | |
| 12991 | | 7 atoms, 6 bonds, 1 residue, 1 model selected |
| 12992 | | |
| 12993 | | > select clear |
| 12994 | | |
| 12995 | | > show #!29 models |
| 12996 | | |
| 12997 | | > hide #!29 models |
| 12998 | | |
| 12999 | | > hide #!57 models |
| 13000 | | |
| 13001 | | > select #65/A:588 |
| 13002 | | |
| 13003 | | 8 atoms, 7 bonds, 1 residue, 1 model selected |
| 13004 | | |
| 13005 | | > delete sel |
| 13006 | | |
| 13007 | | > show #!57 models |
| 13008 | | |
| 13009 | | > fitmap #64 inMap #29.4 |
| 13010 | | |
| 13011 | | No atoms or maps for #64 |
| 13012 | | |
| 13013 | | > fitmap #65 inMap #29.4 |
| 13014 | | |
| 13015 | | Fit molecule copy of copy of CopBprime_O55029.pdb (#65) to map |
| 13016 | | W20_J140_run_body004.mrc (#29.4) using 420 atoms |
| 13017 | | average map value = 0.02083, steps = 56 |
| 13018 | | shifted from previous position = 2.51 |
| 13019 | | rotated from previous position = 13.6 degrees |
| 13020 | | atoms outside contour = 145, contour level = 0.012962 |
| 13021 | | |
| 13022 | | Position of copy of copy of CopBprime_O55029.pdb (#65) relative to |
| 13023 | | W20_J140_run_body004.mrc (#29.4) coordinates: |
| 13024 | | Matrix rotation and translation |
| 13025 | | 0.25505166 0.23428436 0.93811486 140.41746282 |
| 13026 | | 0.81508493 0.46984578 -0.33894174 184.56399264 |
| 13027 | | -0.52017805 0.85109094 -0.07112670 178.31682392 |
| 13028 | | Axis 0.60413780 0.74032411 0.29485206 |
| 13029 | | Axis point 50.19505778 0.00000000 66.66205089 |
| 13030 | | Rotation angle (degrees) 99.96896003 |
| 13031 | | Shift along axis 274.04575231 |
| 13032 | | |
| 13033 | | |
| 13034 | | > show #!29 models |
| 13035 | | |
| 13036 | | > show #!63 models |
| 13037 | | |
| 13038 | | > show #!60 models |
| 13039 | | |
| 13040 | | > show #!61 models |
| 13041 | | |
| 13042 | | > show #!62 models |
| 13043 | | |
| 13044 | | > show #!51 models |
| 13045 | | |
| 13046 | | > show #!52 models |
| 13047 | | |
| 13048 | | > save "/Users/becca/Desktop/Postdoc/COPI notebooks and |
| 13049 | | > resources/Processing/W20_MM_cyto_COPI/20250704_W20_COPI_cyto_alignments.cxs" |
| 13050 | | |
| 13051 | | ——— End of log from Fri Jul 4 14:29:17 2025 ——— |
| 13052 | | |
| 13053 | | > view name session-start |
| 13054 | | |
| 13055 | | opened ChimeraX session |
| 13056 | | |
| 13057 | | > hide #!65 models |
| 13058 | | |
| 13059 | | > hide #!63 models |
| 13060 | | |
| 13061 | | > show #!65 models |
| 13062 | | |
| 13063 | | > hide #!65 models |
| 13064 | | |
| 13065 | | > show #!63 models |
| 13066 | | |
| 13067 | | > show #!65 models |
| 13068 | | |
| 13069 | | > combine #60 #61 #62 |
| 13070 | | |
| 13071 | | Remapping chain ID 'A' in copy of copy of copy of A_fixed_model_2_A.pdb #61 to |
| 13072 | | 'B' |
| 13073 | | Remapping chain ID 'A' in copy of copy of A_fixed_model_2_A.pdb #62 to 'C' |
| 13074 | | |
| 13075 | | > rename #64 alpha-COPI |
| 13076 | | |
| 13077 | | > hide #!64 models |
| 13078 | | |
| 13079 | | > show #!64 models |
| 13080 | | |
| 13081 | | > hide #!64 models |
| 13082 | | |
| 13083 | | > show #!64 models |
| 13084 | | |
| 13085 | | > hide #!64 models |
| 13086 | | |
| 13087 | | > hide #!62 models |
| 13088 | | |
| 13089 | | > hide #!61 models |
| 13090 | | |
| 13091 | | > hide #!60 models |
| 13092 | | |
| 13093 | | > show #!64 models |
| 13094 | | |
| 13095 | | > combine #51 #52 |
| 13096 | | |
| 13097 | | Remapping chain ID 'B' in B_fixed_model_2_B.pdb #52 to 'C' |
| 13098 | | |
| 13099 | | > rename #66 beta-COPI |
| 13100 | | |
| 13101 | | > hide #!66 models |
| 13102 | | |
| 13103 | | > show #!66 models |
| 13104 | | |
| 13105 | | > hide #!66 models |
| 13106 | | |
| 13107 | | > show #!66 models |
| 13108 | | |
| 13109 | | > hide #!66 models |
| 13110 | | |
| 13111 | | > hide #!64 models |
| 13112 | | |
| 13113 | | > show #!64 models |
| 13114 | | |
| 13115 | | > hide #!52 models |
| 13116 | | |
| 13117 | | > hide #!51 models |
| 13118 | | |
| 13119 | | > hide #!65 models |
| 13120 | | |
| 13121 | | > hide #!63 models |
| 13122 | | |
| 13123 | | > hide #!57 models |
| 13124 | | |
| 13125 | | > hide #!53 models |
| 13126 | | |
| 13127 | | > show #!53 models |
| 13128 | | |
| 13129 | | > hide #!53 models |
| 13130 | | |
| 13131 | | > show #!65 models |
| 13132 | | |
| 13133 | | > hide #!65 models |
| 13134 | | |
| 13135 | | > show #!63 models |
| 13136 | | |
| 13137 | | > show #!53 models |
| 13138 | | |
| 13139 | | > hide #!53 models |
| 13140 | | |
| 13141 | | > show #!57 models |
| 13142 | | |
| 13143 | | > show #!53 models |
| 13144 | | |
| 13145 | | > hide #!53 models |
| 13146 | | |
| 13147 | | > show #!65 models |
| 13148 | | |
| 13149 | | > show #!53 models |
| 13150 | | |
| 13151 | | > combine #63 #57 #65 #53 |
| 13152 | | |
| 13153 | | Remapping chain ID 'A' in copy of CopBprime_O55029.pdb #57 to 'B' |
| 13154 | | Remapping chain ID 'A' in copy of copy of CopBprime_O55029.pdb #65 to 'C' |
| 13155 | | Remapping chain ID 'A' in copy of CopBprime_O55029.pdb #53 to 'D' |
| 13156 | | |
| 13157 | | > rename #67 betaprime-COPI |
| 13158 | | |
| 13159 | | > hide #!53 models |
| 13160 | | |
| 13161 | | > hide #!57 models |
| 13162 | | |
| 13163 | | > hide #!63 models |
| 13164 | | |
| 13165 | | > hide #!65 models |
| 13166 | | |
| 13167 | | > show #!66 models |
| 13168 | | |
| 13169 | | > save "/Users/becca/Desktop/Postdoc/COPI notebooks and |
| 13170 | | > resources/Processing/W20_MM_cyto_COPI/20250704_W20_COPI_cyto_alignments.cxs" |
| 13171 | | |
| 13172 | | > show #!37 models |
| 13173 | | |
| 13174 | | > show #!38 models |
| 13175 | | |
| 13176 | | > hide #!38 models |
| 13177 | | |
| 13178 | | > hide #!37 models |
| 13179 | | |
| 13180 | | > show #!50.8 models |
| 13181 | | |
| 13182 | | > show #50.9 models |
| 13183 | | |
| 13184 | | > hide #!64 models |
| 13185 | | |
| 13186 | | > hide #!66 models |
| 13187 | | |
| 13188 | | > hide #!67 models |
| 13189 | | |
| 13190 | | > select add #50.9 |
| 13191 | | |
| 13192 | | 319 atoms, 320 bonds, 37 residues, 1 model selected |
| 13193 | | |
| 13194 | | > fitmap #50.9 inMap #29.4 |
| 13195 | | |
| 13196 | | Fit molecule W20_C1_initial_model_no_wat_lig.pdb A8 (#50.9) to map |
| 13197 | | W20_J140_run_body004.mrc (#29.4) using 319 atoms |
| 13198 | | average map value = 0.01618, steps = 332 |
| 13199 | | shifted from previous position = 5.67 |
| 13200 | | rotated from previous position = 32.6 degrees |
| 13201 | | atoms outside contour = 181, contour level = 0.012962 |
| 13202 | | |
| 13203 | | Position of W20_C1_initial_model_no_wat_lig.pdb A8 (#50.9) relative to |
| 13204 | | W20_J140_run_body004.mrc (#29.4) coordinates: |
| 13205 | | Matrix rotation and translation |
| 13206 | | -0.96507080 0.08189036 0.24886207 278.79886608 |
| 13207 | | -0.17377809 -0.91094231 -0.37414608 529.42927332 |
| 13208 | | 0.19606003 -0.40432423 0.89335457 -19.89239692 |
| 13209 | | Axis -0.11483207 0.20091912 -0.97285410 |
| 13210 | | Axis point 157.88995888 252.84037243 0.00000000 |
| 13211 | | Rotation angle (degrees) 172.44942844 |
| 13212 | | Shift along axis 93.70981152 |
| 13213 | | |
| 13214 | | |
| 13215 | | > select subtract #50.9 |
| 13216 | | |
| 13217 | | Nothing selected |
| 13218 | | |
| 13219 | | > combine #37 |
| 13220 | | |
| 13221 | | [Repeated 1 time(s)] |
| 13222 | | |
| 13223 | | > hide #!69 models |
| 13224 | | |
| 13225 | | > hide #50.9 models |
| 13226 | | |
| 13227 | | > hide #!29 models |
| 13228 | | |
| 13229 | | > ui mousemode right select |
| 13230 | | |
| 13231 | | Drag select of 11 residues |
| 13232 | | |
| 13233 | | > select up |
| 13234 | | |
| 13235 | | 319 atoms, 320 bonds, 37 residues, 1 model selected |
| 13236 | | |
| 13237 | | > delete sel |
| 13238 | | |
| 13239 | | > fitmap #68 inMap #29.1 |
| 13240 | | |
| 13241 | | Fit molecule copy of CopD_full_Q5XJY5.pdb (#68) to map |
| 13242 | | W20_J140_run_body001.mrc (#29.1) using 1112 atoms |
| 13243 | | average map value = 0.01419, steps = 64 |
| 13244 | | shifted from previous position = 0.82 |
| 13245 | | rotated from previous position = 2.64 degrees |
| 13246 | | atoms outside contour = 514, contour level = 0.01322 |
| 13247 | | |
| 13248 | | Position of copy of CopD_full_Q5XJY5.pdb (#68) relative to |
| 13249 | | W20_J140_run_body001.mrc (#29.1) coordinates: |
| 13250 | | Matrix rotation and translation |
| 13251 | | -0.11662970 -0.98563516 0.12215086 180.96364645 |
| 13252 | | -0.16309929 -0.10231295 -0.98129031 185.65988338 |
| 13253 | | 0.97969185 -0.13437031 -0.14882367 140.80476056 |
| 13254 | | Axis 0.58040695 -0.58768567 0.56369614 |
| 13255 | | Axis point 145.90273812 0.00000000 210.04228211 |
| 13256 | | Rotation angle (degrees) 133.14783703 |
| 13257 | | Shift along axis 75.29400436 |
| 13258 | | |
| 13259 | | |
| 13260 | | > hide #!68 models |
| 13261 | | |
| 13262 | | > hide #!50.8 models |
| 13263 | | |
| 13264 | | > show #50.9 models |
| 13265 | | |
| 13266 | | > show #!69 models |
| 13267 | | |
| 13268 | | > ui tool show Matchmaker |
| 13269 | | |
| 13270 | | > matchmaker #!69 to #50.9 |
| 13271 | | |
| 13272 | | Parameters |
| 13273 | | --- |
| 13274 | | Chain pairing | bb |
| 13275 | | Alignment algorithm | Needleman-Wunsch |
| 13276 | | Similarity matrix | BLOSUM-62 |
| 13277 | | SS fraction | 0.3 |
| 13278 | | Gap open (HH/SS/other) | 18/18/6 |
| 13279 | | Gap extend | 1 |
| 13280 | | SS matrix | | | H | S | O |
| 13281 | | ---|---|---|--- |
| 13282 | | H | 6 | -9 | -6 |
| 13283 | | S | | 6 | -6 |
| 13284 | | O | | | 4 |
| 13285 | | Iteration cutoff | 2 |
| 13286 | | |
| 13287 | | Matchmaker W20_C1_initial_model_no_wat_lig.pdb A8, chain A8 (#50.9) with copy |
| 13288 | | of CopD_full_Q5XJY5.pdb, chain A (#69), sequence alignment score = 189.1 |
| 13289 | | RMSD between 37 pruned atom pairs is 0.000 angstroms; (across all 37 pairs: |
| 13290 | | 0.000) |
| 13291 | | |
| 13292 | | Drag select of 135 residues |
| 13293 | | |
| 13294 | | > select up |
| 13295 | | |
| 13296 | | 1112 atoms, 1129 bonds, 138 residues, 1 model selected |
| 13297 | | |
| 13298 | | > delete sel |
| 13299 | | |
| 13300 | | > show #!68 models |
| 13301 | | |
| 13302 | | > hide #50.9 models |
| 13303 | | |
| 13304 | | > select clear |
| 13305 | | |
| 13306 | | [Repeated 1 time(s)] |
| 13307 | | |
| 13308 | | > select #68/A:138 |
| 13309 | | |
| 13310 | | 6 atoms, 5 bonds, 1 residue, 1 model selected |
| 13311 | | |
| 13312 | | > show sel atoms |
| 13313 | | |
| 13314 | | > style sel stick |
| 13315 | | |
| 13316 | | Changed 6 atom styles |
| 13317 | | |
| 13318 | | > hide sel cartoons |
| 13319 | | |
| 13320 | | > color sel byhetero |
| 13321 | | |
| 13322 | | > select #69/A:139 |
| 13323 | | |
| 13324 | | 10 atoms, 10 bonds, 1 residue, 1 model selected |
| 13325 | | |
| 13326 | | > show sel atoms |
| 13327 | | |
| 13328 | | > hide sel cartoons |
| 13329 | | |
| 13330 | | > color sel byhetero |
| 13331 | | |
| 13332 | | > select clear |
| 13333 | | |
| 13334 | | > ui tool show "Build Structure" |
| 13335 | | |
| 13336 | | > select #68/A:138@C |
| 13337 | | |
| 13338 | | 1 atom, 1 residue, 1 model selected |
| 13339 | | |
| 13340 | | > select add #69/A:139@N |
| 13341 | | |
| 13342 | | 2 atoms, 2 residues, 2 models selected |
| 13343 | | |
| 13344 | | > build join peptide sel length 1.33 omega 180 phi -120 move small |
| 13345 | | |
| 13346 | | > undo |
| 13347 | | |
| 13348 | | Undo failed, probably because structures have been modified. |
| 13349 | | |
| 13350 | | > show #!29 models |
| 13351 | | |
| 13352 | | > build join peptide sel length 1.33 omega 90 phi -120 move small |
| 13353 | | |
| 13354 | | No chain-terminal carbons in atoms |
| 13355 | | |
| 13356 | | > hide #!29 models |
| 13357 | | |
| 13358 | | Must select exactly 3 atoms in graphics window |
| 13359 | | |
| 13360 | | > select #68/A:138@CA |
| 13361 | | |
| 13362 | | 1 atom, 1 residue, 1 model selected |
| 13363 | | |
| 13364 | | > select #68/A:138@C |
| 13365 | | |
| 13366 | | 1 atom, 1 residue, 1 model selected |
| 13367 | | |
| 13368 | | > select add #68/A:138@CA |
| 13369 | | |
| 13370 | | 2 atoms, 1 residue, 1 model selected |
| 13371 | | |
| 13372 | | > select add #68/A:139@N |
| 13373 | | |
| 13374 | | 3 atoms, 2 residues, 1 model selected |
| 13375 | | Cannot set the angle if the end atoms have a connection that does not pass |
| 13376 | | through the center atom |
| 13377 | | |
| 13378 | | > select clear |
| 13379 | | |
| 13380 | | > select #68/A:138@CA |
| 13381 | | |
| 13382 | | 1 atom, 1 residue, 1 model selected |
| 13383 | | |
| 13384 | | > select add #68/A:138@C |
| 13385 | | |
| 13386 | | 2 atoms, 1 residue, 1 model selected |
| 13387 | | |
| 13388 | | > select add #68/A:139@N |
| 13389 | | |
| 13390 | | 3 atoms, 2 residues, 1 model selected |
| 13391 | | |
| 13392 | | > show #!29 models |
| 13393 | | |
| 13394 | | > angle #68:138@CA@C:139@N 137.7 |
| 13395 | | |
| 13396 | | > angle #68:138@CA@C:139@N 91 |
| 13397 | | |
| 13398 | | > angle #68:138@CA@C:139@N 67.2 |
| 13399 | | |
| 13400 | | > angle #68:138@CA@C:139@N 110.2 |
| 13401 | | |
| 13402 | | > select clear |
| 13403 | | |
| 13404 | | > select #68/A:152 |
| 13405 | | |
| 13406 | | 9 atoms, 8 bonds, 1 residue, 1 model selected |
| 13407 | | |
| 13408 | | > select up |
| 13409 | | |
| 13410 | | 319 atoms, 320 bonds, 37 residues, 1 model selected |
| 13411 | | |
| 13412 | | > ui mousemode right "translate selected atoms" |
| 13413 | | |
| 13414 | | > select add #68 |
| 13415 | | |
| 13416 | | 1431 atoms, 1450 bonds, 175 residues, 1 model selected |
| 13417 | | |
| 13418 | | > select subtract #68 |
| 13419 | | |
| 13420 | | Nothing selected |
| 13421 | | |
| 13422 | | > ui mousemode right tug |
| 13423 | | |
| 13424 | | > ui mousemode right select |
| 13425 | | |
| 13426 | | > select #68/A:155 |
| 13427 | | |
| 13428 | | 5 atoms, 4 bonds, 1 residue, 1 model selected |
| 13429 | | |
| 13430 | | > select up |
| 13431 | | |
| 13432 | | 319 atoms, 320 bonds, 37 residues, 1 model selected |
| 13433 | | |
| 13434 | | > ui mousemode right tug |
| 13435 | | |
| 13436 | | > ui mousemode right "bond rotation" |
| 13437 | | |
| 13438 | | > ui mousemode right select |
| 13439 | | |
| 13440 | | > ui mousemode right "play coordinates" |
| 13441 | | |
| 13442 | | > select add #68 |
| 13443 | | |
| 13444 | | 1431 atoms, 1450 bonds, 175 residues, 1 model selected |
| 13445 | | |
| 13446 | | > select subtract #68 |
| 13447 | | |
| 13448 | | Nothing selected |
| 13449 | | |
| 13450 | | > hide #!68 models |
| 13451 | | |
| 13452 | | > close #68 |
| 13453 | | |
| 13454 | | > combine 37 |
| 13455 | | |
| 13456 | | Expected a keyword |
| 13457 | | |
| 13458 | | > combine #37 |
| 13459 | | |
| 13460 | | > delete sel |
| 13461 | | |
| 13462 | | > combine #37 |
| 13463 | | |
| 13464 | | > hide #!69 models |
| 13465 | | |
| 13466 | | > hide #!29 models |
| 13467 | | |
| 13468 | | > ui mousemode right select |
| 13469 | | |
| 13470 | | Drag select of 7 residues |
| 13471 | | |
| 13472 | | > select up |
| 13473 | | |
| 13474 | | 319 atoms, 320 bonds, 37 residues, 1 model selected |
| 13475 | | |
| 13476 | | > combine #37 |
| 13477 | | |
| 13478 | | > delete sel |
| 13479 | | |
| 13480 | | > close #70 |
| 13481 | | |
| 13482 | | > hide #!68 models |
| 13483 | | |
| 13484 | | > ui tool show Matchmaker |
| 13485 | | |
| 13486 | | > matchmaker #!69 to #50.9 |
| 13487 | | |
| 13488 | | Parameters |
| 13489 | | --- |
| 13490 | | Chain pairing | bb |
| 13491 | | Alignment algorithm | Needleman-Wunsch |
| 13492 | | Similarity matrix | BLOSUM-62 |
| 13493 | | SS fraction | 0.3 |
| 13494 | | Gap open (HH/SS/other) | 18/18/6 |
| 13495 | | Gap extend | 1 |
| 13496 | | SS matrix | | | H | S | O |
| 13497 | | ---|---|---|--- |
| 13498 | | H | 6 | -9 | -6 |
| 13499 | | S | | 6 | -6 |
| 13500 | | O | | | 4 |
| 13501 | | Iteration cutoff | 2 |
| 13502 | | |
| 13503 | | Matchmaker W20_C1_initial_model_no_wat_lig.pdb A8, chain A8 (#50.9) with copy |
| 13504 | | of CopD_full_Q5XJY5.pdb, chain A (#69), sequence alignment score = 189.1 |
| 13505 | | RMSD between 37 pruned atom pairs is 0.000 angstroms; (across all 37 pairs: |
| 13506 | | 0.000) |
| 13507 | | |
| 13508 | | |
| 13509 | | > show #!69 models |
| 13510 | | |
| 13511 | | Drag select of 103 residues |
| 13512 | | |
| 13513 | | > select up |
| 13514 | | |
| 13515 | | 936 atoms, 951 bonds, 116 residues, 1 model selected |
| 13516 | | Drag select of 135 residues |
| 13517 | | |
| 13518 | | > select up |
| 13519 | | |
| 13520 | | 1112 atoms, 1129 bonds, 138 residues, 1 model selected |
| 13521 | | |
| 13522 | | > delete sel |
| 13523 | | |
| 13524 | | > show #!29 models |
| 13525 | | |
| 13526 | | > show #!68 models |
| 13527 | | |
| 13528 | | > hide #!29 models |
| 13529 | | |
| 13530 | | > select #69/A:168 |
| 13531 | | |
| 13532 | | 9 atoms, 8 bonds, 1 residue, 1 model selected |
| 13533 | | |
| 13534 | | > select clear |
| 13535 | | |
| 13536 | | > select #69/A:165 |
| 13537 | | |
| 13538 | | 9 atoms, 8 bonds, 1 residue, 1 model selected |
| 13539 | | |
| 13540 | | > select clear |
| 13541 | | |
| 13542 | | > select #69/A:162 |
| 13543 | | |
| 13544 | | 9 atoms, 8 bonds, 1 residue, 1 model selected |
| 13545 | | |
| 13546 | | > show sel atoms |
| 13547 | | |
| 13548 | | > select #69/A:164 |
| 13549 | | |
| 13550 | | 9 atoms, 8 bonds, 1 residue, 1 model selected |
| 13551 | | |
| 13552 | | > show sel atoms |
| 13553 | | |
| 13554 | | > show #!29 models |
| 13555 | | |
| 13556 | | > hide #!29 models |
| 13557 | | |
| 13558 | | > show #!30 models |
| 13559 | | |
| 13560 | | > select #69/A:165 |
| 13561 | | |
| 13562 | | 9 atoms, 8 bonds, 1 residue, 1 model selected |
| 13563 | | |
| 13564 | | > show sel atoms |
| 13565 | | |
| 13566 | | > color #30.1 #92929280 models |
| 13567 | | |
| 13568 | | > color #30.4 #92929263 models |
| 13569 | | |
| 13570 | | > show #!67 models |
| 13571 | | |
| 13572 | | > select add #69 |
| 13573 | | |
| 13574 | | 319 atoms, 320 bonds, 37 residues, 1 model selected |
| 13575 | | |
| 13576 | | > hide sel atoms |
| 13577 | | |
| 13578 | | > select subtract #69 |
| 13579 | | |
| 13580 | | Nothing selected |
| 13581 | | |
| 13582 | | > combine #68 #69 |
| 13583 | | |
| 13584 | | Remapping chain ID 'A' in copy of CopD_full_Q5XJY5.pdb #69 to 'B' |
| 13585 | | |
| 13586 | | > rename #70 delta-COPI |
| 13587 | | |
| 13588 | | > hide #!69 models |
| 13589 | | |
| 13590 | | > hide #!68 models |
| 13591 | | |
| 13592 | | > hide #!30.1 models |
| 13593 | | |
| 13594 | | > show #!30.1 models |
| 13595 | | |
| 13596 | | > hide #!30 models |
| 13597 | | |
| 13598 | | > show #!29 models |
| 13599 | | |
| 13600 | | > show #!66 models |
| 13601 | | |
| 13602 | | > show #!64 models |
| 13603 | | |
| 13604 | | > show #39 models |
| 13605 | | |
| 13606 | | > hide #!29 models |
| 13607 | | |
| 13608 | | > show #!33 models |
| 13609 | | |
| 13610 | | > hide #!33 models |
| 13611 | | |
| 13612 | | > show #!32 models |
| 13613 | | |
| 13614 | | > hide #!32 models |
| 13615 | | |
| 13616 | | > show #!33 models |
| 13617 | | |
| 13618 | | > fitmap #39 inMap #33.3 |
| 13619 | | |
| 13620 | | Fit molecule CopZ1_P61924.pdb (#39) to map W20_J160_run_ct6_body003.mrc |
| 13621 | | (#33.3) using 1420 atoms |
| 13622 | | average map value = 0.0138, steps = 80 |
| 13623 | | shifted from previous position = 0.00922 |
| 13624 | | rotated from previous position = 0.0243 degrees |
| 13625 | | atoms outside contour = 767, contour level = 0.015167 |
| 13626 | | |
| 13627 | | Position of CopZ1_P61924.pdb (#39) relative to W20_J160_run_ct6_body003.mrc |
| 13628 | | (#33.3) coordinates: |
| 13629 | | Matrix rotation and translation |
| 13630 | | -0.59905768 0.35670401 0.71686272 128.55067540 |
| 13631 | | 0.33940715 -0.69775395 0.63082663 127.78309606 |
| 13632 | | 0.72521218 0.62120986 0.29692697 93.14263724 |
| 13633 | | Axis -0.44771433 -0.38871406 -0.80526596 |
| 13634 | | Axis point 38.57235221 41.25427436 0.00000000 |
| 13635 | | Rotation angle (degrees) 179.38464020 |
| 13636 | | Shift along axis -182.22965962 |
| 13637 | | |
| 13638 | | |
| 13639 | | > hide #39 models |
| 13640 | | |
| 13641 | | > show #39 models |
| 13642 | | |
| 13643 | | > combine #39 |
| 13644 | | |
| 13645 | | > rename #71 zeta-COPI |
| 13646 | | |
| 13647 | | > show #56 models |
| 13648 | | |
| 13649 | | > hide #56 models |
| 13650 | | |
| 13651 | | > show #!55 models |
| 13652 | | |
| 13653 | | > hide #!55 models |
| 13654 | | |
| 13655 | | > show #!38 models |
| 13656 | | |
| 13657 | | > save "/Users/becca/Desktop/Postdoc/COPI notebooks and |
| 13658 | | > resources/Processing/W20_MM_cyto_COPI/20250704_W20_COPI_cyto_alignments.cxs" |
| 13659 | | |
| 13660 | | > hide #!38 models |
| 13661 | | |
| 13662 | | > show #!50.4 models |
| 13663 | | |
| 13664 | | > hide #!50.4 models |
| 13665 | | |
| 13666 | | > show #!50.4 models |
| 13667 | | |
| 13668 | | > hide #!50.4 models |
| 13669 | | |
| 13670 | | > show #!50.4 models |
| 13671 | | |
| 13672 | | > hide #!50.4 models |
| 13673 | | |
| 13674 | | > show #50.1 models |
| 13675 | | |
| 13676 | | > show #!50.2 models |
| 13677 | | |
| 13678 | | > hide #!50.2 models |
| 13679 | | |
| 13680 | | > show #!50.3 models |
| 13681 | | |
| 13682 | | > hide #!50.3 models |
| 13683 | | |
| 13684 | | > show #!50.4 models |
| 13685 | | |
| 13686 | | > hide #!50.4 models |
| 13687 | | |
| 13688 | | > show #!50.5 models |
| 13689 | | |
| 13690 | | > hide #!50.5 models |
| 13691 | | |
| 13692 | | > show #50.6 models |
| 13693 | | |
| 13694 | | > hide #50.6 models |
| 13695 | | |
| 13696 | | > show #!50.7 models |
| 13697 | | |
| 13698 | | > hide #!50.7 models |
| 13699 | | |
| 13700 | | > show #!50.8 models |
| 13701 | | |
| 13702 | | > hide #!50.8 models |
| 13703 | | |
| 13704 | | > show #50.9 models |
| 13705 | | |
| 13706 | | > hide #50.9 models |
| 13707 | | |
| 13708 | | > show #!50.10 models |
| 13709 | | |
| 13710 | | > select add #50.10 |
| 13711 | | |
| 13712 | | 4143 atoms, 4205 bonds, 2 pseudobonds, 528 residues, 2 models selected |
| 13713 | | Drag select of 33.3 W20_J160_run_ct6_body003.mrc , 4 residues |
| 13714 | | |
| 13715 | | > select add #50.10 |
| 13716 | | |
| 13717 | | 4143 atoms, 4205 bonds, 2 pseudobonds, 528 residues, 4 models selected |
| 13718 | | |
| 13719 | | > ui mousemode right "translate selected models" |
| 13720 | | |
| 13721 | | > view matrix models |
| 13722 | | > #33.3,0.99304,0.059881,0.10144,5.194,-0.064889,0.99679,0.046802,13.668,-0.098315,-0.053059,0.99374,40.329,#50.10,-0.92962,0.23756,-0.28172,353.7,-0.021762,-0.79854,-0.60155,498.49,-0.36787,-0.55308,0.74751,143.01 |
| 13723 | | |
| 13724 | | > undo |
| 13725 | | |
| 13726 | | [Repeated 1 time(s)] |
| 13727 | | |
| 13728 | | > ui mousemode right select |
| 13729 | | |
| 13730 | | > select clear |
| 13731 | | |
| 13732 | | > open "/Users/becca/Desktop/Postdoc/COPI notebooks and resources/Structure |
| 13733 | | > files/alphafold/CopG1_ Q9QZE5.pdb" |
| 13734 | | |
| 13735 | | CopG1_ Q9QZE5.pdb title: |
| 13736 | | Alphafold monomer V2.0 prediction for coatomer subunit γ-1 (Q9QZE5) [more |
| 13737 | | info...] |
| 13738 | | |
| 13739 | | Chain information for CopG1_ Q9QZE5.pdb #72 |
| 13740 | | --- |
| 13741 | | Chain | Description | UniProt |
| 13742 | | A | coatomer subunit γ-1 | COPG1_MOUSE 1-874 |
| 13743 | | |
| 13744 | | Computing secondary structure |
| 13745 | | |
| 13746 | | > select add #72 |
| 13747 | | |
| 13748 | | 6830 atoms, 6947 bonds, 874 residues, 1 model selected |
| 13749 | | |
| 13750 | | > ui tool show Matchmaker |
| 13751 | | |
| 13752 | | > matchmaker #72 to #50.10 |
| 13753 | | |
| 13754 | | Parameters |
| 13755 | | --- |
| 13756 | | Chain pairing | bb |
| 13757 | | Alignment algorithm | Needleman-Wunsch |
| 13758 | | Similarity matrix | BLOSUM-62 |
| 13759 | | SS fraction | 0.3 |
| 13760 | | Gap open (HH/SS/other) | 18/18/6 |
| 13761 | | Gap extend | 1 |
| 13762 | | SS matrix | | | H | S | O |
| 13763 | | ---|---|---|--- |
| 13764 | | H | 6 | -9 | -6 |
| 13765 | | S | | 6 | -6 |
| 13766 | | O | | | 4 |
| 13767 | | Iteration cutoff | 2 |
| 13768 | | |
| 13769 | | Matchmaker W20_C1_initial_model_no_wat_lig.pdb A9, chain A9 (#50.10) with |
| 13770 | | CopG1_ Q9QZE5.pdb, chain A (#72), sequence alignment score = 2728.3 |
| 13771 | | RMSD between 527 pruned atom pairs is 0.000 angstroms; (across all 528 pairs: |
| 13772 | | 0.471) |
| 13773 | | |
| 13774 | | |
| 13775 | | > select subtract #72 |
| 13776 | | |
| 13777 | | Nothing selected |
| 13778 | | |
| 13779 | | > hide #!64 models |
| 13780 | | |
| 13781 | | > hide #!66 models |
| 13782 | | |
| 13783 | | > hide #!67 models |
| 13784 | | |
| 13785 | | > hide #!70 models |
| 13786 | | |
| 13787 | | > hide #71 models |
| 13788 | | |
| 13789 | | > hide #39 models |
| 13790 | | |
| 13791 | | > color #72 gammav3 |
| 13792 | | |
| 13793 | | > fitmap #72 inMap #33.3 |
| 13794 | | |
| 13795 | | Fit molecule CopG1_ Q9QZE5.pdb (#72) to map W20_J160_run_ct6_body003.mrc |
| 13796 | | (#33.3) using 6830 atoms |
| 13797 | | average map value = 0.008592, steps = 128 |
| 13798 | | shifted from previous position = 5.94 |
| 13799 | | rotated from previous position = 20.6 degrees |
| 13800 | | atoms outside contour = 5149, contour level = 0.015167 |
| 13801 | | |
| 13802 | | Position of CopG1_ Q9QZE5.pdb (#72) relative to W20_J160_run_ct6_body003.mrc |
| 13803 | | (#33.3) coordinates: |
| 13804 | | Matrix rotation and translation |
| 13805 | | -0.21262269 0.89025762 0.40278153 115.23719127 |
| 13806 | | -0.39923844 0.29708069 -0.86738211 108.04417936 |
| 13807 | | -0.89185215 -0.34523099 0.29225897 114.80605117 |
| 13808 | | Axis 0.27475861 0.68124291 -0.67853946 |
| 13809 | | Axis point 106.74618671 0.00000000 53.93689380 |
| 13810 | | Rotation angle (degrees) 108.15818379 |
| 13811 | | Shift along axis 27.36630527 |
| 13812 | | |
| 13813 | | |
| 13814 | | > hide #!50.10 models |
| 13815 | | |
| 13816 | | > hide #50.1 models |
| 13817 | | |
| 13818 | | > hide #72 models |
| 13819 | | |
| 13820 | | > show #!41 models |
| 13821 | | |
| 13822 | | > show #40 models |
| 13823 | | |
| 13824 | | > hide #40 models |
| 13825 | | |
| 13826 | | > hide #!41 models |
| 13827 | | |
| 13828 | | > open "/Users/becca/Desktop/Postdoc/COPI notebooks and resources/Alphafold |
| 13829 | | > predictions/alphafold 3 |
| 13830 | | > /fold_20240724_copbdg1z1_try2/fold_20240724_copbdg1z1_try2_model_0.cif" |
| 13831 | | |
| 13832 | | Chain information for fold_20240724_copbdg1z1_try2_model_0.cif #73 |
| 13833 | | --- |
| 13834 | | Chain | Description |
| 13835 | | A | . |
| 13836 | | B | . |
| 13837 | | C | . |
| 13838 | | D | . |
| 13839 | | |
| 13840 | | Computing secondary structure |
| 13841 | | |
| 13842 | | > ui tool show Matchmaker |
| 13843 | | |
| 13844 | | > matchmaker #73 to #72 |
| 13845 | | |
| 13846 | | Parameters |
| 13847 | | --- |
| 13848 | | Chain pairing | bb |
| 13849 | | Alignment algorithm | Needleman-Wunsch |
| 13850 | | Similarity matrix | BLOSUM-62 |
| 13851 | | SS fraction | 0.3 |
| 13852 | | Gap open (HH/SS/other) | 18/18/6 |
| 13853 | | Gap extend | 1 |
| 13854 | | SS matrix | | | H | S | O |
| 13855 | | ---|---|---|--- |
| 13856 | | H | 6 | -9 | -6 |
| 13857 | | S | | 6 | -6 |
| 13858 | | O | | | 4 |
| 13859 | | Iteration cutoff | 2 |
| 13860 | | |
| 13861 | | Matchmaker CopG1_ Q9QZE5.pdb, chain A (#72) with |
| 13862 | | fold_20240724_copbdg1z1_try2_model_0.cif, chain C (#73), sequence alignment |
| 13863 | | score = 4461 |
| 13864 | | RMSD between 219 pruned atom pairs is 1.225 angstroms; (across all 874 pairs: |
| 13865 | | 21.353) |
| 13866 | | |
| 13867 | | |
| 13868 | | > hide #!50 models |
| 13869 | | |
| 13870 | | > show #!50 models |
| 13871 | | |
| 13872 | | > hide #!33 models |
| 13873 | | |
| 13874 | | > select clear |
| 13875 | | |
| 13876 | | > select #73/B:154 |
| 13877 | | |
| 13878 | | 9 atoms, 8 bonds, 1 residue, 1 model selected |
| 13879 | | |
| 13880 | | > select up |
| 13881 | | |
| 13882 | | 319 atoms, 320 bonds, 37 residues, 1 model selected |
| 13883 | | |
| 13884 | | > select up |
| 13885 | | |
| 13886 | | 4014 atoms, 4083 bonds, 511 residues, 1 model selected |
| 13887 | | |
| 13888 | | > delete sel |
| 13889 | | |
| 13890 | | > select #73/A:242 |
| 13891 | | |
| 13892 | | 11 atoms, 10 bonds, 1 residue, 1 model selected |
| 13893 | | |
| 13894 | | > select up |
| 13895 | | |
| 13896 | | 49 atoms, 49 bonds, 6 residues, 1 model selected |
| 13897 | | |
| 13898 | | > select up |
| 13899 | | |
| 13900 | | 7501 atoms, 7619 bonds, 953 residues, 1 model selected |
| 13901 | | |
| 13902 | | > delete sel |
| 13903 | | |
| 13904 | | > fitmap #73 inMap #33.3 |
| 13905 | | |
| 13906 | | Fit molecule fold_20240724_copbdg1z1_try2_model_0.cif (#73) to map |
| 13907 | | W20_J160_run_ct6_body003.mrc (#33.3) using 8250 atoms |
| 13908 | | average map value = 0.008274, steps = 184 |
| 13909 | | shifted from previous position = 16.4 |
| 13910 | | rotated from previous position = 27.6 degrees |
| 13911 | | atoms outside contour = 6288, contour level = 0.015167 |
| 13912 | | |
| 13913 | | Position of fold_20240724_copbdg1z1_try2_model_0.cif (#73) relative to |
| 13914 | | W20_J160_run_ct6_body003.mrc (#33.3) coordinates: |
| 13915 | | Matrix rotation and translation |
| 13916 | | 0.89001705 -0.00769161 -0.45586235 140.75280440 |
| 13917 | | 0.39379749 -0.49091643 0.77712585 125.72122596 |
| 13918 | | -0.22976767 -0.87117271 -0.43389506 123.79108229 |
| 13919 | | Axis -0.96307771 -0.13210395 0.23458445 |
| 13920 | | Axis point 0.00000000 97.90843398 46.99023466 |
| 13921 | | Rotation angle (degrees) 121.15782367 |
| 13922 | | Shift along axis -123.12469702 |
| 13923 | | |
| 13924 | | |
| 13925 | | > show #!33 models |
| 13926 | | |
| 13927 | | > show #71 models |
| 13928 | | |
| 13929 | | > hide #71 models |
| 13930 | | |
| 13931 | | > show #71 models |
| 13932 | | |
| 13933 | | > hide #71 models |
| 13934 | | |
| 13935 | | > show #71 models |
| 13936 | | |
| 13937 | | > hide #71 models |
| 13938 | | |
| 13939 | | > hide #!33 models |
| 13940 | | |
| 13941 | | > select #73/D:47 |
| 13942 | | |
| 13943 | | 8 atoms, 7 bonds, 1 residue, 1 model selected |
| 13944 | | |
| 13945 | | > select up |
| 13946 | | |
| 13947 | | 128 atoms, 129 bonds, 15 residues, 1 model selected |
| 13948 | | |
| 13949 | | > select up |
| 13950 | | |
| 13951 | | 1420 atoms, 1441 bonds, 177 residues, 1 model selected |
| 13952 | | |
| 13953 | | > delete sel |
| 13954 | | |
| 13955 | | > show #!33 models |
| 13956 | | |
| 13957 | | > fitmap #73 inMap #33.3 |
| 13958 | | |
| 13959 | | Fit molecule fold_20240724_copbdg1z1_try2_model_0.cif (#73) to map |
| 13960 | | W20_J160_run_ct6_body003.mrc (#33.3) using 6830 atoms |
| 13961 | | average map value = 0.007849, steps = 80 |
| 13962 | | shifted from previous position = 1.21 |
| 13963 | | rotated from previous position = 2.15 degrees |
| 13964 | | atoms outside contour = 5293, contour level = 0.015167 |
| 13965 | | |
| 13966 | | Position of fold_20240724_copbdg1z1_try2_model_0.cif (#73) relative to |
| 13967 | | W20_J160_run_ct6_body003.mrc (#33.3) coordinates: |
| 13968 | | Matrix rotation and translation |
| 13969 | | 0.89436621 -0.03216667 -0.44617753 141.60252778 |
| 13970 | | 0.36827977 -0.51322863 0.77522022 126.38957440 |
| 13971 | | -0.25392733 -0.85764892 -0.44716801 122.18980164 |
| 13972 | | Axis -0.96493218 -0.11360886 0.23664092 |
| 13973 | | Axis point 0.00000000 98.06597121 46.27860492 |
| 13974 | | Rotation angle (degrees) 122.20940815 |
| 13975 | | Shift along axis -122.08070325 |
| 13976 | | |
| 13977 | | |
| 13978 | | > show #35 models |
| 13979 | | |
| 13980 | | > close #73 |
| 13981 | | |
| 13982 | | > open "/Users/becca/Desktop/Postdoc/COPI notebooks and resources/Alphafold |
| 13983 | | > predictions/alphafold 3 |
| 13984 | | > /fold_20240711_copi_golph3/fold_20240711_copi_golph3_model_0.cif" |
| 13985 | | |
| 13986 | | Chain information for fold_20240711_copi_golph3_model_0.cif #73 |
| 13987 | | --- |
| 13988 | | Chain | Description |
| 13989 | | A | . |
| 13990 | | B C | . |
| 13991 | | D | . |
| 13992 | | E | . |
| 13993 | | F | . |
| 13994 | | G | . |
| 13995 | | H | . |
| 13996 | | I | . |
| 13997 | | |
| 13998 | | |
| 13999 | | > ui tool show Matchmaker |
| 14000 | | |
| 14001 | | > matchmaker #73 to #72 |
| 14002 | | |
| 14003 | | Computing secondary structure |
| 14004 | | Parameters |
| 14005 | | --- |
| 14006 | | Chain pairing | bb |
| 14007 | | Alignment algorithm | Needleman-Wunsch |
| 14008 | | Similarity matrix | BLOSUM-62 |
| 14009 | | SS fraction | 0.3 |
| 14010 | | Gap open (HH/SS/other) | 18/18/6 |
| 14011 | | Gap extend | 1 |
| 14012 | | SS matrix | | | H | S | O |
| 14013 | | ---|---|---|--- |
| 14014 | | H | 6 | -9 | -6 |
| 14015 | | S | | 6 | -6 |
| 14016 | | O | | | 4 |
| 14017 | | Iteration cutoff | 2 |
| 14018 | | |
| 14019 | | Matchmaker CopG1_ Q9QZE5.pdb, chain A (#72) with |
| 14020 | | fold_20240711_copi_golph3_model_0.cif, chain H (#73), sequence alignment score |
| 14021 | | = 3086.5 |
| 14022 | | RMSD between 216 pruned atom pairs is 1.190 angstroms; (across all 609 pairs: |
| 14023 | | 15.751) |
| 14024 | | |
| 14025 | | |
| 14026 | | > hide #35,73 atoms |
| 14027 | | |
| 14028 | | > hide #35,73 cartoons |
| 14029 | | |
| 14030 | | > show #35,73 cartoons |
| 14031 | | |
| 14032 | | > hide #35 models |
| 14033 | | |
| 14034 | | > hide #!33 models |
| 14035 | | |
| 14036 | | > select #73/C:142 |
| 14037 | | |
| 14038 | | 9 atoms, 8 bonds, 1 residue, 1 model selected |
| 14039 | | |
| 14040 | | > select up |
| 14041 | | |
| 14042 | | 59 atoms, 58 bonds, 8 residues, 1 model selected |
| 14043 | | |
| 14044 | | > select up |
| 14045 | | |
| 14046 | | 1457 atoms, 1483 bonds, 181 residues, 1 model selected |
| 14047 | | |
| 14048 | | > delete sel |
| 14049 | | |
| 14050 | | > split #73 chains |
| 14051 | | |
| 14052 | | Split fold_20240711_copi_golph3_model_0.cif (#73) into 8 models |
| 14053 | | Chain information for fold_20240711_copi_golph3_model_0.cif A #73.1 |
| 14054 | | --- |
| 14055 | | Chain | Description |
| 14056 | | A | No description available |
| 14057 | | |
| 14058 | | Chain information for fold_20240711_copi_golph3_model_0.cif B #73.2 |
| 14059 | | --- |
| 14060 | | Chain | Description |
| 14061 | | B | No description available |
| 14062 | | |
| 14063 | | Chain information for fold_20240711_copi_golph3_model_0.cif D #73.3 |
| 14064 | | --- |
| 14065 | | Chain | Description |
| 14066 | | D | No description available |
| 14067 | | |
| 14068 | | Chain information for fold_20240711_copi_golph3_model_0.cif E #73.4 |
| 14069 | | --- |
| 14070 | | Chain | Description |
| 14071 | | E | No description available |
| 14072 | | |
| 14073 | | Chain information for fold_20240711_copi_golph3_model_0.cif F #73.5 |
| 14074 | | --- |
| 14075 | | Chain | Description |
| 14076 | | F | No description available |
| 14077 | | |
| 14078 | | Chain information for fold_20240711_copi_golph3_model_0.cif G #73.6 |
| 14079 | | --- |
| 14080 | | Chain | Description |
| 14081 | | G | No description available |
| 14082 | | |
| 14083 | | Chain information for fold_20240711_copi_golph3_model_0.cif H #73.7 |
| 14084 | | --- |
| 14085 | | Chain | Description |
| 14086 | | H | No description available |
| 14087 | | |
| 14088 | | Chain information for fold_20240711_copi_golph3_model_0.cif I #73.8 |
| 14089 | | --- |
| 14090 | | Chain | Description |
| 14091 | | I | No description available |
| 14092 | | |
| 14093 | | |
| 14094 | | > hide #73.1 models |
| 14095 | | |
| 14096 | | > show #73.1 models |
| 14097 | | |
| 14098 | | > close #73.1 |
| 14099 | | |
| 14100 | | > hide #!73 models |
| 14101 | | |
| 14102 | | > show #!73 models |
| 14103 | | |
| 14104 | | > hide #73.2 models |
| 14105 | | |
| 14106 | | > show #73.2 models |
| 14107 | | |
| 14108 | | > close #73.2 |
| 14109 | | |
| 14110 | | > hide #73.3 models |
| 14111 | | |
| 14112 | | > show #73.3 models |
| 14113 | | |
| 14114 | | > close #73.3 |
| 14115 | | |
| 14116 | | > hide #73.4 models |
| 14117 | | |
| 14118 | | > show #73.4 models |
| 14119 | | |
| 14120 | | > hide #73.5 models |
| 14121 | | |
| 14122 | | > show #73.5 models |
| 14123 | | |
| 14124 | | > close #73.5 |
| 14125 | | |
| 14126 | | > close #73.6 |
| 14127 | | |
| 14128 | | > show #!33 models |
| 14129 | | |
| 14130 | | > close #73.4 |
| 14131 | | |
| 14132 | | > show #!50.10 models |
| 14133 | | |
| 14134 | | > hide #!50.10 models |
| 14135 | | |
| 14136 | | > show #50.9 models |
| 14137 | | |
| 14138 | | > hide #50.9 models |
| 14139 | | |
| 14140 | | > show #50.9 models |
| 14141 | | |
| 14142 | | > hide #50.9 models |
| 14143 | | |
| 14144 | | > show #50.1 models |
| 14145 | | |
| 14146 | | > hide #50.1 models |
| 14147 | | |
| 14148 | | > show #71 models |
| 14149 | | |
| 14150 | | > close #73.8 |
| 14151 | | |
| 14152 | | > combine #73.7 |
| 14153 | | |
| 14154 | | > hide #73.7 models |
| 14155 | | |
| 14156 | | > hide #!73 models |
| 14157 | | |
| 14158 | | > hide #71 models |
| 14159 | | |
| 14160 | | > hide #!33 models |
| 14161 | | |
| 14162 | | > show #!33 models |
| 14163 | | |
| 14164 | | > select #74/H:609 |
| 14165 | | |
| 14166 | | 12 atoms, 11 bonds, 1 residue, 1 model selected |
| 14167 | | |
| 14168 | | > select up |
| 14169 | | |
| 14170 | | 227 atoms, 230 bonds, 31 residues, 1 model selected |
| 14171 | | |
| 14172 | | > delete sel |
| 14173 | | |
| 14174 | | > select #74/H:564 |
| 14175 | | |
| 14176 | | 9 atoms, 8 bonds, 1 residue, 1 model selected |
| 14177 | | |
| 14178 | | > select up |
| 14179 | | |
| 14180 | | 103 atoms, 104 bonds, 13 residues, 1 model selected |
| 14181 | | |
| 14182 | | > select up |
| 14183 | | |
| 14184 | | 4525 atoms, 4596 bonds, 578 residues, 1 model selected |
| 14185 | | |
| 14186 | | > select clear |
| 14187 | | |
| 14188 | | > select #74/H:353 |
| 14189 | | |
| 14190 | | 6 atoms, 5 bonds, 1 residue, 1 model selected |
| 14191 | | |
| 14192 | | > hide #!33 models |
| 14193 | | |
| 14194 | | Drag select of 109 residues |
| 14195 | | |
| 14196 | | > select up |
| 14197 | | |
| 14198 | | 939 atoms, 952 bonds, 119 residues, 1 model selected |
| 14199 | | |
| 14200 | | > delete sel |
| 14201 | | |
| 14202 | | Drag select of 27 residues, 2 pseudobonds |
| 14203 | | |
| 14204 | | > delete sel |
| 14205 | | |
| 14206 | | > fitmap #74 inMap #33.3 |
| 14207 | | |
| 14208 | | Fit molecule copy of fold_20240711_copi_golph3_model_0.cif H (#74) to map |
| 14209 | | W20_J160_run_ct6_body003.mrc (#33.3) using 3367 atoms |
| 14210 | | average map value = 0.01504, steps = 104 |
| 14211 | | shifted from previous position = 1.23 |
| 14212 | | rotated from previous position = 8.87 degrees |
| 14213 | | atoms outside contour = 1844, contour level = 0.015167 |
| 14214 | | |
| 14215 | | Position of copy of fold_20240711_copi_golph3_model_0.cif H (#74) relative to |
| 14216 | | W20_J160_run_ct6_body003.mrc (#33.3) coordinates: |
| 14217 | | Matrix rotation and translation |
| 14218 | | -0.99039171 0.12782412 -0.05277549 148.12639654 |
| 14219 | | 0.13807487 0.93531006 -0.32577664 131.22576391 |
| 14220 | | 0.00771933 -0.32993345 -0.94397263 135.30516062 |
| 14221 | | Axis -0.06759286 -0.98369069 0.16668480 |
| 14222 | | Axis point 68.34524384 0.00000000 79.86683576 |
| 14223 | | Rotation angle (degrees) 178.23793985 |
| 14224 | | Shift along axis -116.54453594 |
| 14225 | | |
| 14226 | | |
| 14227 | | > show #!33 models |
| 14228 | | |
| 14229 | | > hide #!33 models |
| 14230 | | |
| 14231 | | Drag select of 37 residues |
| 14232 | | |
| 14233 | | > select up |
| 14234 | | |
| 14235 | | 343 atoms, 348 bonds, 42 residues, 1 model selected |
| 14236 | | |
| 14237 | | > delete sel |
| 14238 | | |
| 14239 | | > fitmap #74 inMap #33.3 |
| 14240 | | |
| 14241 | | Fit molecule copy of fold_20240711_copi_golph3_model_0.cif H (#74) to map |
| 14242 | | W20_J160_run_ct6_body003.mrc (#33.3) using 3024 atoms |
| 14243 | | average map value = 0.01541, steps = 84 |
| 14244 | | shifted from previous position = 0.341 |
| 14245 | | rotated from previous position = 2.61 degrees |
| 14246 | | atoms outside contour = 1604, contour level = 0.015167 |
| 14247 | | |
| 14248 | | Position of copy of fold_20240711_copi_golph3_model_0.cif H (#74) relative to |
| 14249 | | W20_J160_run_ct6_body003.mrc (#33.3) coordinates: |
| 14250 | | Matrix rotation and translation |
| 14251 | | -0.99016649 0.13961225 -0.00887325 145.93626541 |
| 14252 | | 0.13499683 0.93694534 -0.32234963 131.14942473 |
| 14253 | | -0.03669021 -0.32037766 -0.94657909 136.23220850 |
| 14254 | | Axis 0.06976430 0.98410929 -0.16328456 |
| 14255 | | Axis point 68.87722497 0.00000000 78.50166031 |
| 14256 | | Rotation angle (degrees) 179.19020792 |
| 14257 | | Shift along axis 117.00189352 |
| 14258 | | |
| 14259 | | |
| 14260 | | > show #!33 models |
| 14261 | | |
| 14262 | | > hide #!33 models |
| 14263 | | |
| 14264 | | Drag select of 7 residues |
| 14265 | | |
| 14266 | | > select up |
| 14267 | | |
| 14268 | | 130 atoms, 130 bonds, 17 residues, 1 model selected |
| 14269 | | Drag select of 5 residues |
| 14270 | | |
| 14271 | | > select down |
| 14272 | | |
| 14273 | | 44 atoms, 5 residues, 1 model selected |
| 14274 | | |
| 14275 | | > select up |
| 14276 | | |
| 14277 | | 142 atoms, 143 bonds, 18 residues, 1 model selected |
| 14278 | | |
| 14279 | | > delete sel |
| 14280 | | |
| 14281 | | > fitmap #74 inMap #33.3 |
| 14282 | | |
| 14283 | | Fit molecule copy of fold_20240711_copi_golph3_model_0.cif H (#74) to map |
| 14284 | | W20_J160_run_ct6_body003.mrc (#33.3) using 2882 atoms |
| 14285 | | average map value = 0.01543, steps = 60 |
| 14286 | | shifted from previous position = 0.199 |
| 14287 | | rotated from previous position = 0.43 degrees |
| 14288 | | atoms outside contour = 1520, contour level = 0.015167 |
| 14289 | | |
| 14290 | | Position of copy of fold_20240711_copi_golph3_model_0.cif H (#74) relative to |
| 14291 | | W20_J160_run_ct6_body003.mrc (#33.3) coordinates: |
| 14292 | | Matrix rotation and translation |
| 14293 | | -0.99012237 0.14014794 -0.00403046 145.70159327 |
| 14294 | | 0.13418777 0.93889954 -0.31695629 130.92767817 |
| 14295 | | -0.04063657 -0.31436635 -0.94843158 136.20145596 |
| 14296 | | Axis 0.06966230 0.98460506 -0.16031230 |
| 14297 | | Axis point 68.95076353 0.00000000 78.11025829 |
| 14298 | | Rotation angle (degrees) 178.93485378 |
| 14299 | | Shift along axis 117.22719466 |
| 14300 | | |
| 14301 | | |
| 14302 | | > show #!33 models |
| 14303 | | |
| 14304 | | > show #!50.10 models |
| 14305 | | |
| 14306 | | > hide #!50 models |
| 14307 | | |
| 14308 | | > show #!50 models |
| 14309 | | |
| 14310 | | > hide #!33 models |
| 14311 | | |
| 14312 | | > combine #50.10 |
| 14313 | | |
| 14314 | | > hide #!50.10 models |
| 14315 | | |
| 14316 | | > hide #!74 models |
| 14317 | | |
| 14318 | | > show #!74 models |
| 14319 | | |
| 14320 | | > hide #!74 models |
| 14321 | | |
| 14322 | | Drag select of 113 residues |
| 14323 | | |
| 14324 | | > select up |
| 14325 | | |
| 14326 | | 1028 atoms, 1042 bonds, 130 residues, 1 model selected |
| 14327 | | |
| 14328 | | > delete sel |
| 14329 | | |
| 14330 | | > fitmap #75 inMap #33.3 |
| 14331 | | |
| 14332 | | Fit molecule copy of W20_C1_initial_model_no_wat_lig.pdb A9 (#75) to map |
| 14333 | | W20_J160_run_ct6_body003.mrc (#33.3) using 3115 atoms |
| 14334 | | average map value = 0.01389, steps = 132 |
| 14335 | | shifted from previous position = 1.68 |
| 14336 | | rotated from previous position = 15.8 degrees |
| 14337 | | atoms outside contour = 1847, contour level = 0.015167 |
| 14338 | | |
| 14339 | | Position of copy of W20_C1_initial_model_no_wat_lig.pdb A9 (#75) relative to |
| 14340 | | W20_J160_run_ct6_body003.mrc (#33.3) coordinates: |
| 14341 | | Matrix rotation and translation |
| 14342 | | -0.96975860 0.09758407 -0.22370877 404.94223752 |
| 14343 | | 0.05855198 -0.79680528 -0.60139256 482.79146988 |
| 14344 | | -0.23693866 -0.59630420 0.76699503 131.62487193 |
| 14345 | | Axis 0.12253380 0.31859145 -0.93993880 |
| 14346 | | Axis point 213.57688590 261.52023160 0.00000000 |
| 14347 | | Rotation angle (degrees) 178.81027642 |
| 14348 | | Shift along axis 79.71302152 |
| 14349 | | |
| 14350 | | |
| 14351 | | > show #!33 models |
| 14352 | | |
| 14353 | | > open "/Users/becca/Desktop/Postdoc/Grants and paper drafts/Golph3 |
| 14354 | | > paper/Final maps and model/Model_files/20250328_COPI_GOLPH3_model_v2.cif" |
| 14355 | | |
| 14356 | | Chain information for 20250328_COPI_GOLPH3_model_v2.cif #76 |
| 14357 | | --- |
| 14358 | | Chain | Description |
| 14359 | | A | No description available |
| 14360 | | B | No description available |
| 14361 | | D | No description available |
| 14362 | | E | No description available |
| 14363 | | F | No description available |
| 14364 | | G | No description available |
| 14365 | | H | No description available |
| 14366 | | I | No description available |
| 14367 | | J | No description available |
| 14368 | | K | No description available |
| 14369 | | L | No description available |
| 14370 | | M | No description available |
| 14371 | | N | No description available |
| 14372 | | O | No description available |
| 14373 | | P | No description available |
| 14374 | | R S T | No description available |
| 14375 | | |
| 14376 | | |
| 14377 | | > hide #76#!75 atoms |
| 14378 | | |
| 14379 | | > show #76#!75 cartoons |
| 14380 | | |
| 14381 | | > ui tool show Matchmaker |
| 14382 | | |
| 14383 | | > matchmaker #76 to #66 |
| 14384 | | |
| 14385 | | Parameters |
| 14386 | | --- |
| 14387 | | Chain pairing | bb |
| 14388 | | Alignment algorithm | Needleman-Wunsch |
| 14389 | | Similarity matrix | BLOSUM-62 |
| 14390 | | SS fraction | 0.3 |
| 14391 | | Gap open (HH/SS/other) | 18/18/6 |
| 14392 | | Gap extend | 1 |
| 14393 | | SS matrix | | | H | S | O |
| 14394 | | ---|---|---|--- |
| 14395 | | H | 6 | -9 | -6 |
| 14396 | | S | | 6 | -6 |
| 14397 | | O | | | 4 |
| 14398 | | Iteration cutoff | 2 |
| 14399 | | |
| 14400 | | Matchmaker beta-COPI, chain B (#66) with 20250328_COPI_GOLPH3_model_v2.cif, |
| 14401 | | chain J (#76), sequence alignment score = 1591 |
| 14402 | | RMSD between 180 pruned atom pairs is 0.463 angstroms; (across all 289 pairs: |
| 14403 | | 3.827) |
| 14404 | | |
| 14405 | | |
| 14406 | | > show #!66 models |
| 14407 | | |
| 14408 | | > hide #!66 models |
| 14409 | | |
| 14410 | | > show #!66 models |
| 14411 | | |
| 14412 | | > hide #76 models |
| 14413 | | |
| 14414 | | > show #76 models |
| 14415 | | |
| 14416 | | > hide #76 models |
| 14417 | | |
| 14418 | | > show #76 models |
| 14419 | | |
| 14420 | | > select add #76 |
| 14421 | | |
| 14422 | | 17013 atoms, 16995 bonds, 4252 residues, 1 model selected |
| 14423 | | |
| 14424 | | > select subtract #76 |
| 14425 | | |
| 14426 | | Nothing selected |
| 14427 | | |
| 14428 | | > hide #76 models |
| 14429 | | |
| 14430 | | > hide #!75 models |
| 14431 | | |
| 14432 | | > show #76 models |
| 14433 | | |
| 14434 | | > select add #76 |
| 14435 | | |
| 14436 | | 17013 atoms, 16995 bonds, 4252 residues, 1 model selected |
| 14437 | | |
| 14438 | | > ui mousemode right "translate selected models" |
| 14439 | | |
| 14440 | | > view matrix models |
| 14441 | | > #76,-0.94328,0.33002,0.036298,249.11,-0.21754,-0.53176,-0.81848,562.73,-0.25081,-0.77995,0.57339,257.94 |
| 14442 | | |
| 14443 | | > fitmap #76 inMap #33.1 |
| 14444 | | |
| 14445 | | Fit molecule 20250328_COPI_GOLPH3_model_v2.cif (#76) to map |
| 14446 | | W20_J160_run_ct6_body001.mrc (#33.1) using 17013 atoms |
| 14447 | | average map value = 0.005784, steps = 248 |
| 14448 | | shifted from previous position = 23.7 |
| 14449 | | rotated from previous position = 25.1 degrees |
| 14450 | | atoms outside contour = 13846, contour level = 0.014811 |
| 14451 | | |
| 14452 | | Position of 20250328_COPI_GOLPH3_model_v2.cif (#76) relative to |
| 14453 | | W20_J160_run_ct6_body001.mrc (#33.1) coordinates: |
| 14454 | | Matrix rotation and translation |
| 14455 | | -0.95309148 0.15118983 -0.26221799 379.07308450 |
| 14456 | | 0.09927921 -0.66223841 -0.74268696 486.62957335 |
| 14457 | | -0.28593754 -0.73388141 0.61616378 222.17176604 |
| 14458 | | Axis 0.15248160 0.41074018 -0.89891149 |
| 14459 | | Axis point 212.09731096 291.09961203 0.00000000 |
| 14460 | | Rotation angle (degrees) 178.34540300 |
| 14461 | | Shift along axis 57.96723338 |
| 14462 | | |
| 14463 | | |
| 14464 | | > select subtract #76 |
| 14465 | | |
| 14466 | | Nothing selected |
| 14467 | | |
| 14468 | | > ui tool show Matchmaker |
| 14469 | | |
| 14470 | | > matchmaker #76 to #66 |
| 14471 | | |
| 14472 | | Parameters |
| 14473 | | --- |
| 14474 | | Chain pairing | bb |
| 14475 | | Alignment algorithm | Needleman-Wunsch |
| 14476 | | Similarity matrix | BLOSUM-62 |
| 14477 | | SS fraction | 0.3 |
| 14478 | | Gap open (HH/SS/other) | 18/18/6 |
| 14479 | | Gap extend | 1 |
| 14480 | | SS matrix | | | H | S | O |
| 14481 | | ---|---|---|--- |
| 14482 | | H | 6 | -9 | -6 |
| 14483 | | S | | 6 | -6 |
| 14484 | | O | | | 4 |
| 14485 | | Iteration cutoff | 2 |
| 14486 | | |
| 14487 | | Matchmaker beta-COPI, chain B (#66) with 20250328_COPI_GOLPH3_model_v2.cif, |
| 14488 | | chain J (#76), sequence alignment score = 1591 |
| 14489 | | RMSD between 180 pruned atom pairs is 0.463 angstroms; (across all 289 pairs: |
| 14490 | | 3.827) |
| 14491 | | |
| 14492 | | |
| 14493 | | > undo |
| 14494 | | |
| 14495 | | > view matrix models |
| 14496 | | > #76,-0.94328,0.33002,0.036298,269.75,-0.21754,-0.53176,-0.81848,549.29,-0.25081,-0.77995,0.57339,234.77 |
| 14497 | | |
| 14498 | | > select subtract #76 |
| 14499 | | |
| 14500 | | Nothing selected |
| 14501 | | |
| 14502 | | > select add #76 |
| 14503 | | |
| 14504 | | 17013 atoms, 16995 bonds, 4252 residues, 1 model selected |
| 14505 | | |
| 14506 | | > view matrix models |
| 14507 | | > #76,-0.94328,0.33002,0.036298,244.4,-0.21754,-0.53176,-0.81848,557.07,-0.25081,-0.77995,0.57339,249.76 |
| 14508 | | |
| 14509 | | > show #!51 models |
| 14510 | | |
| 14511 | | > hide #!51 models |
| 14512 | | |
| 14513 | | > show #!52 models |
| 14514 | | |
| 14515 | | > hide #!52 models |
| 14516 | | |
| 14517 | | > hide #!66 models |
| 14518 | | |
| 14519 | | > show #!52 models |
| 14520 | | |
| 14521 | | > hide #!52 models |
| 14522 | | |
| 14523 | | > show #!51 models |
| 14524 | | |
| 14525 | | > hide #!51 models |
| 14526 | | |
| 14527 | | > ui tool show Matchmaker |
| 14528 | | |
| 14529 | | > matchmaker #76 to #51 |
| 14530 | | |
| 14531 | | Parameters |
| 14532 | | --- |
| 14533 | | Chain pairing | bb |
| 14534 | | Alignment algorithm | Needleman-Wunsch |
| 14535 | | Similarity matrix | BLOSUM-62 |
| 14536 | | SS fraction | 0.3 |
| 14537 | | Gap open (HH/SS/other) | 18/18/6 |
| 14538 | | Gap extend | 1 |
| 14539 | | SS matrix | | | H | S | O |
| 14540 | | ---|---|---|--- |
| 14541 | | H | 6 | -9 | -6 |
| 14542 | | S | | 6 | -6 |
| 14543 | | O | | | 4 |
| 14544 | | Iteration cutoff | 2 |
| 14545 | | |
| 14546 | | Matchmaker copy of B_fixed_model_2_B.pdb, chain B (#51) with |
| 14547 | | 20250328_COPI_GOLPH3_model_v2.cif, chain J (#76), sequence alignment score = |
| 14548 | | 1591 |
| 14549 | | RMSD between 180 pruned atom pairs is 0.463 angstroms; (across all 289 pairs: |
| 14550 | | 3.827) |
| 14551 | | |
| 14552 | | |
| 14553 | | > view matrix models |
| 14554 | | > #76,-0.94328,0.33002,0.036298,250.65,-0.21754,-0.53176,-0.81848,564.67,-0.25081,-0.77995,0.57339,248.23 |
| 14555 | | |
| 14556 | | > fitmap #76 inMap #33.1 |
| 14557 | | |
| 14558 | | Fit molecule 20250328_COPI_GOLPH3_model_v2.cif (#76) to map |
| 14559 | | W20_J160_run_ct6_body001.mrc (#33.1) using 17013 atoms |
| 14560 | | average map value = 0.005784, steps = 256 |
| 14561 | | shifted from previous position = 20.4 |
| 14562 | | rotated from previous position = 25.2 degrees |
| 14563 | | atoms outside contour = 13847, contour level = 0.014811 |
| 14564 | | |
| 14565 | | Position of 20250328_COPI_GOLPH3_model_v2.cif (#76) relative to |
| 14566 | | W20_J160_run_ct6_body001.mrc (#33.1) coordinates: |
| 14567 | | Matrix rotation and translation |
| 14568 | | -0.95306184 0.15114897 -0.26234924 379.12122640 |
| 14569 | | 0.09940762 -0.66223994 -0.74266842 486.60244827 |
| 14570 | | -0.28599171 -0.73388844 0.61613026 222.19665492 |
| 14571 | | Axis 0.15253453 0.41074061 -0.89890231 |
| 14572 | | Axis point 212.11797169 291.10158267 0.00000000 |
| 14573 | | Rotation angle (degrees) 178.35078282 |
| 14574 | | Shift along axis 57.96337940 |
| 14575 | | |
| 14576 | | |
| 14577 | | > select subtract #76 |
| 14578 | | |
| 14579 | | Nothing selected |
| 14580 | | |
| 14581 | | > hide #!50 models |
| 14582 | | |
| 14583 | | > show #!50 models |
| 14584 | | |
| 14585 | | > hide #!33 models |
| 14586 | | |
| 14587 | | > ui mousemode right select |
| 14588 | | |
| 14589 | | > select clear |
| 14590 | | |
| 14591 | | > select #76/F:486 |
| 14592 | | |
| 14593 | | 4 atoms, 3 bonds, 1 residue, 1 model selected |
| 14594 | | |
| 14595 | | > select up |
| 14596 | | |
| 14597 | | 24 atoms, 23 bonds, 6 residues, 1 model selected |
| 14598 | | |
| 14599 | | > select up |
| 14600 | | |
| 14601 | | 2344 atoms, 2343 bonds, 586 residues, 1 model selected |
| 14602 | | |
| 14603 | | > select up |
| 14604 | | |
| 14605 | | 17013 atoms, 16995 bonds, 4252 residues, 1 model selected |
| 14606 | | |
| 14607 | | > select down |
| 14608 | | |
| 14609 | | 2344 atoms, 2343 bonds, 586 residues, 1 model selected |
| 14610 | | |
| 14611 | | > delete sel |
| 14612 | | |
| 14613 | | > select #76/L:158 |
| 14614 | | |
| 14615 | | 4 atoms, 3 bonds, 1 residue, 1 model selected |
| 14616 | | |
| 14617 | | > select clear |
| 14618 | | |
| 14619 | | Drag select of 234 residues |
| 14620 | | |
| 14621 | | > select clear |
| 14622 | | |
| 14623 | | Drag select of 67 residues |
| 14624 | | |
| 14625 | | > select up |
| 14626 | | |
| 14627 | | 544 atoms, 538 bonds, 136 residues, 1 model selected |
| 14628 | | |
| 14629 | | > select up |
| 14630 | | |
| 14631 | | 1940 atoms, 1938 bonds, 485 residues, 1 model selected |
| 14632 | | |
| 14633 | | > delete sel |
| 14634 | | |
| 14635 | | > select #76/T:111 |
| 14636 | | |
| 14637 | | 4 atoms, 3 bonds, 1 residue, 1 model selected |
| 14638 | | |
| 14639 | | > select up |
| 14640 | | |
| 14641 | | 48 atoms, 47 bonds, 12 residues, 1 model selected |
| 14642 | | |
| 14643 | | > select up |
| 14644 | | |
| 14645 | | 669 atoms, 668 bonds, 167 residues, 1 model selected |
| 14646 | | |
| 14647 | | > delete sel |
| 14648 | | |
| 14649 | | > select clear |
| 14650 | | |
| 14651 | | [Repeated 1 time(s)] |
| 14652 | | |
| 14653 | | > select #76/L:243 |
| 14654 | | |
| 14655 | | 4 atoms, 3 bonds, 1 residue, 1 model selected |
| 14656 | | |
| 14657 | | > select up |
| 14658 | | |
| 14659 | | 48 atoms, 47 bonds, 12 residues, 1 model selected |
| 14660 | | |
| 14661 | | > select up |
| 14662 | | |
| 14663 | | 804 atoms, 803 bonds, 201 residues, 1 model selected |
| 14664 | | |
| 14665 | | > delete sel |
| 14666 | | |
| 14667 | | > select #76/K:689 |
| 14668 | | |
| 14669 | | 4 atoms, 3 bonds, 1 residue, 1 model selected |
| 14670 | | |
| 14671 | | > select up |
| 14672 | | |
| 14673 | | 40 atoms, 39 bonds, 10 residues, 1 model selected |
| 14674 | | |
| 14675 | | > select up |
| 14676 | | |
| 14677 | | 204 atoms, 203 bonds, 51 residues, 1 model selected |
| 14678 | | |
| 14679 | | > delete sel |
| 14680 | | |
| 14681 | | > select clear |
| 14682 | | |
| 14683 | | > select #76/J:133 |
| 14684 | | |
| 14685 | | 4 atoms, 3 bonds, 1 residue, 1 model selected |
| 14686 | | Drag select of 60 residues |
| 14687 | | |
| 14688 | | > select clear |
| 14689 | | |
| 14690 | | Drag select of 34 residues |
| 14691 | | |
| 14692 | | > select up |
| 14693 | | |
| 14694 | | 204 atoms, 200 bonds, 51 residues, 1 model selected |
| 14695 | | |
| 14696 | | > select up |
| 14697 | | |
| 14698 | | 1244 atoms, 1243 bonds, 311 residues, 1 model selected |
| 14699 | | |
| 14700 | | > delete sel |
| 14701 | | |
| 14702 | | > select clear |
| 14703 | | |
| 14704 | | > select #76/S:91 |
| 14705 | | |
| 14706 | | 4 atoms, 3 bonds, 1 residue, 1 model selected |
| 14707 | | |
| 14708 | | > select up |
| 14709 | | |
| 14710 | | 28 atoms, 27 bonds, 7 residues, 1 model selected |
| 14711 | | |
| 14712 | | > select up |
| 14713 | | |
| 14714 | | 669 atoms, 668 bonds, 167 residues, 1 model selected |
| 14715 | | |
| 14716 | | > delete sel |
| 14717 | | |
| 14718 | | > select #76/R:138 |
| 14719 | | |
| 14720 | | 4 atoms, 3 bonds, 1 residue, 1 model selected |
| 14721 | | |
| 14722 | | > select up |
| 14723 | | |
| 14724 | | 32 atoms, 31 bonds, 8 residues, 1 model selected |
| 14725 | | |
| 14726 | | > select up |
| 14727 | | |
| 14728 | | 669 atoms, 668 bonds, 167 residues, 1 model selected |
| 14729 | | |
| 14730 | | > delete sel |
| 14731 | | |
| 14732 | | > select clear |
| 14733 | | |
| 14734 | | > select #76/H:729 |
| 14735 | | |
| 14736 | | 4 atoms, 3 bonds, 1 residue, 1 model selected |
| 14737 | | |
| 14738 | | > select #76/H:727 |
| 14739 | | |
| 14740 | | 4 atoms, 3 bonds, 1 residue, 1 model selected |
| 14741 | | |
| 14742 | | > select clear |
| 14743 | | |
| 14744 | | Drag select of 12 residues |
| 14745 | | |
| 14746 | | > select clear |
| 14747 | | |
| 14748 | | Drag select of 63 residues |
| 14749 | | |
| 14750 | | > select up |
| 14751 | | |
| 14752 | | 432 atoms, 427 bonds, 108 residues, 1 model selected |
| 14753 | | |
| 14754 | | > select up |
| 14755 | | |
| 14756 | | 3337 atoms, 3335 bonds, 834 residues, 1 model selected |
| 14757 | | |
| 14758 | | > delete sel |
| 14759 | | |
| 14760 | | Drag select of 61 residues |
| 14761 | | |
| 14762 | | > select up |
| 14763 | | |
| 14764 | | 509 atoms, 503 bonds, 127 residues, 1 model selected |
| 14765 | | |
| 14766 | | > select up |
| 14767 | | |
| 14768 | | 977 atoms, 976 bonds, 244 residues, 1 model selected |
| 14769 | | |
| 14770 | | > delete sel |
| 14771 | | |
| 14772 | | > show #!33 models |
| 14773 | | |
| 14774 | | > hide #76 models |
| 14775 | | |
| 14776 | | > show #76 models |
| 14777 | | |
| 14778 | | > show #!75 models |
| 14779 | | |
| 14780 | | > hide #!75 models |
| 14781 | | |
| 14782 | | > show #!74 models |
| 14783 | | |
| 14784 | | > hide #76 models |
| 14785 | | |
| 14786 | | > show #76 models |
| 14787 | | |
| 14788 | | > hide #!74 models |
| 14789 | | |
| 14790 | | > show #!74 models |
| 14791 | | |
| 14792 | | > hide #76 models |
| 14793 | | |
| 14794 | | > hide #!50 models |
| 14795 | | |
| 14796 | | > show #76 models |
| 14797 | | |
| 14798 | | > hide #!33 models |
| 14799 | | |
| 14800 | | > select clear |
| 14801 | | |
| 14802 | | > select #76/I:400 |
| 14803 | | |
| 14804 | | 4 atoms, 3 bonds, 1 residue, 1 model selected |
| 14805 | | |
| 14806 | | > select up |
| 14807 | | |
| 14808 | | 64 atoms, 63 bonds, 16 residues, 1 model selected |
| 14809 | | |
| 14810 | | > select up |
| 14811 | | |
| 14812 | | 1252 atoms, 1251 bonds, 313 residues, 1 model selected |
| 14813 | | |
| 14814 | | > delete sel |
| 14815 | | |
| 14816 | | > show #50.9 models |
| 14817 | | |
| 14818 | | > hide #50.9 models |
| 14819 | | |
| 14820 | | > show #!50.10 models |
| 14821 | | |
| 14822 | | > hide #!50.10 models |
| 14823 | | |
| 14824 | | > show #!50.10 models |
| 14825 | | |
| 14826 | | > hide #!50.10 models |
| 14827 | | |
| 14828 | | > show #50.9 models |
| 14829 | | |
| 14830 | | > hide #50.9 models |
| 14831 | | |
| 14832 | | > show #!50.8 models |
| 14833 | | |
| 14834 | | > hide #!50.8 models |
| 14835 | | |
| 14836 | | > show #72 models |
| 14837 | | |
| 14838 | | > hide #72 models |
| 14839 | | |
| 14840 | | > show #72 models |
| 14841 | | |
| 14842 | | > hide #72 models |
| 14843 | | |
| 14844 | | > show #!75 models |
| 14845 | | |
| 14846 | | > hide #!75 models |
| 14847 | | |
| 14848 | | > hide #76 models |
| 14849 | | |
| 14850 | | > show #76 models |
| 14851 | | |
| 14852 | | > hide #!74 models |
| 14853 | | |
| 14854 | | > show #!75 models |
| 14855 | | |
| 14856 | | > hide #!75 models |
| 14857 | | |
| 14858 | | > select #76/A:21 |
| 14859 | | |
| 14860 | | 4 atoms, 3 bonds, 1 residue, 1 model selected |
| 14861 | | |
| 14862 | | > select up |
| 14863 | | |
| 14864 | | 36 atoms, 35 bonds, 9 residues, 1 model selected |
| 14865 | | |
| 14866 | | > select up |
| 14867 | | |
| 14868 | | 64 atoms, 63 bonds, 16 residues, 1 model selected |
| 14869 | | |
| 14870 | | > delete sel |
| 14871 | | |
| 14872 | | Drag select of 15 residues |
| 14873 | | |
| 14874 | | > select up |
| 14875 | | |
| 14876 | | 116 atoms, 112 bonds, 29 residues, 1 model selected |
| 14877 | | |
| 14878 | | > select up |
| 14879 | | |
| 14880 | | 580 atoms, 579 bonds, 145 residues, 1 model selected |
| 14881 | | |
| 14882 | | > delete sel |
| 14883 | | |
| 14884 | | > show #!75 models |
| 14885 | | |
| 14886 | | > hide #!75 models |
| 14887 | | |
| 14888 | | > show #!74 models |
| 14889 | | |
| 14890 | | > show #!33 models |
| 14891 | | |
| 14892 | | > select clear |
| 14893 | | |
| 14894 | | > select #76/O:107 |
| 14895 | | |
| 14896 | | 4 atoms, 3 bonds, 1 residue, 1 model selected |
| 14897 | | |
| 14898 | | > select up |
| 14899 | | |
| 14900 | | 48 atoms, 47 bonds, 12 residues, 1 model selected |
| 14901 | | |
| 14902 | | > select up |
| 14903 | | |
| 14904 | | 1132 atoms, 1131 bonds, 283 residues, 1 model selected |
| 14905 | | |
| 14906 | | > delete sel |
| 14907 | | |
| 14908 | | > hide #!33 models |
| 14909 | | |
| 14910 | | > hide #!74 models |
| 14911 | | |
| 14912 | | > show #!74 models |
| 14913 | | |
| 14914 | | > hide #76 models |
| 14915 | | |
| 14916 | | > hide #!74 models |
| 14917 | | |
| 14918 | | > show #76 models |
| 14919 | | |
| 14920 | | > select #76/M:371 |
| 14921 | | |
| 14922 | | 4 atoms, 3 bonds, 1 residue, 1 model selected |
| 14923 | | Drag select of 16 residues |
| 14924 | | |
| 14925 | | > select up |
| 14926 | | |
| 14927 | | 144 atoms, 142 bonds, 36 residues, 1 model selected |
| 14928 | | Drag select of 48 residues |
| 14929 | | |
| 14930 | | > select up |
| 14931 | | |
| 14932 | | 216 atoms, 215 bonds, 54 residues, 1 model selected |
| 14933 | | |
| 14934 | | > delete sel |
| 14935 | | |
| 14936 | | > show #!74 models |
| 14937 | | |
| 14938 | | > show #!33 models |
| 14939 | | |
| 14940 | | > show #!66 models |
| 14941 | | |
| 14942 | | > hide #76 models |
| 14943 | | |
| 14944 | | > hide #!66 models |
| 14945 | | |
| 14946 | | > show #76 models |
| 14947 | | |
| 14948 | | > hide #!33 models |
| 14949 | | |
| 14950 | | > show #!33 models |
| 14951 | | |
| 14952 | | > hide #!33 models |
| 14953 | | |
| 14954 | | > hide #76 models |
| 14955 | | |
| 14956 | | > show #!33 models |
| 14957 | | |
| 14958 | | > show #76 models |
| 14959 | | |
| 14960 | | > hide #!33 models |
| 14961 | | |
| 14962 | | > hide #!74 models |
| 14963 | | |
| 14964 | | > select #76/M:354 |
| 14965 | | |
| 14966 | | 4 atoms, 3 bonds, 1 residue, 1 model selected |
| 14967 | | |
| 14968 | | > select up |
| 14969 | | |
| 14970 | | 12 atoms, 11 bonds, 3 residues, 1 model selected |
| 14971 | | |
| 14972 | | > select up |
| 14973 | | |
| 14974 | | 380 atoms, 379 bonds, 95 residues, 1 model selected |
| 14975 | | |
| 14976 | | > select #76/M:363 |
| 14977 | | |
| 14978 | | 4 atoms, 3 bonds, 1 residue, 1 model selected |
| 14979 | | Drag select of 8 residues |
| 14980 | | |
| 14981 | | > select up |
| 14982 | | |
| 14983 | | 52 atoms, 51 bonds, 13 residues, 1 model selected |
| 14984 | | |
| 14985 | | > delete sel |
| 14986 | | |
| 14987 | | Drag select of 4 residues |
| 14988 | | |
| 14989 | | > select clear |
| 14990 | | |
| 14991 | | Drag select of 5 residues |
| 14992 | | |
| 14993 | | > delete sel |
| 14994 | | |
| 14995 | | > show #!74 models |
| 14996 | | |
| 14997 | | > show #!33 models |
| 14998 | | |
| 14999 | | > save "/Users/becca/Desktop/Postdoc/COPI notebooks and |
| 15000 | | > resources/Processing/W20_MM_cyto_COPI/20250704_W20_COPI_cyto_alignments_v2.cxs" |
| 15001 | | |
| 15002 | | [Repeated 1 time(s)] |
| 15003 | | |
| 15004 | | > save "/Users/becca/Desktop/Postdoc/COPI notebooks and |
| 15005 | | > resources/Processing/W20_MM_cyto_COPI/20250705_W20_COPI_cyto_alignments.cxs" |
| 15006 | | |
| 15007 | | ——— End of log from Sat Jul 5 20:47:49 2025 ——— |
| 15008 | | |
| 15009 | | > view name session-start |
| 15010 | | |
| 15011 | | opened ChimeraX session |
| 15012 | | |
| 15013 | | > open |
| 15014 | | > /Users/becca/Desktop/Postdoc/Phenix/W20_MM_cyto_COPI/PredictAndBuildCryoEM_7/PredictAndBuild_7_overall_best_superposed_predicted_models.pdb |
| 15015 | | |
| 15016 | | Chain information for |
| 15017 | | PredictAndBuild_7_overall_best_superposed_predicted_models.pdb #77 |
| 15018 | | --- |
| 15019 | | Chain | Description |
| 15020 | | A | No description available |
| 15021 | | |
| 15022 | | |
| 15023 | | > open |
| 15024 | | > /Users/becca/Desktop/Postdoc/Phenix/W20_MM_cyto_COPI/PredictAndBuildCryoEM_7/PredictAndBuild_7_overall_best.pdb |
| 15025 | | |
| 15026 | | Chain information for PredictAndBuild_7_overall_best.pdb #78 |
| 15027 | | --- |
| 15028 | | Chain | Description |
| 15029 | | A | No description available |
| 15030 | | |
| 15031 | | |
| 15032 | | > open |
| 15033 | | > /Users/becca/Desktop/Postdoc/Phenix/W20_MM_cyto_COPI/PredictAndBuildCryoEM_7/PredictAndBuild_7_superposed_predicted_untrimmed_cycle_1.pdb |
| 15034 | | |
| 15035 | | Chain information for |
| 15036 | | PredictAndBuild_7_superposed_predicted_untrimmed_cycle_1.pdb #79 |
| 15037 | | --- |
| 15038 | | Chain | Description |
| 15039 | | A | No description available |
| 15040 | | |
| 15041 | | |
| 15042 | | > open |
| 15043 | | > /Users/becca/Desktop/Postdoc/Phenix/W20_MM_cyto_COPI/PredictAndBuildCryoEM_7/PredictAndBuild_7_superposed_predicted_untrimmed_cycle_2.pdb |
| 15044 | | |
| 15045 | | Chain information for |
| 15046 | | PredictAndBuild_7_superposed_predicted_untrimmed_cycle_2.pdb #80 |
| 15047 | | --- |
| 15048 | | Chain | Description |
| 15049 | | A | No description available |
| 15050 | | |
| 15051 | | Computing secondary structure |
| 15052 | | [Repeated 3 time(s)] |
| 15053 | | |
| 15054 | | > ui tool show Matchmaker |
| 15055 | | |
| 15056 | | > hide #!74 models |
| 15057 | | |
| 15058 | | > show #!74 models |
| 15059 | | |
| 15060 | | > hide #!74 models |
| 15061 | | |
| 15062 | | > show #!74 models |
| 15063 | | |
| 15064 | | > matchmaker #78-80#!77 to #74 |
| 15065 | | |
| 15066 | | Parameters |
| 15067 | | --- |
| 15068 | | Chain pairing | bb |
| 15069 | | Alignment algorithm | Needleman-Wunsch |
| 15070 | | Similarity matrix | BLOSUM-62 |
| 15071 | | SS fraction | 0.3 |
| 15072 | | Gap open (HH/SS/other) | 18/18/6 |
| 15073 | | Gap extend | 1 |
| 15074 | | SS matrix | | | H | S | O |
| 15075 | | ---|---|---|--- |
| 15076 | | H | 6 | -9 | -6 |
| 15077 | | S | | 6 | -6 |
| 15078 | | O | | | 4 |
| 15079 | | Iteration cutoff | 2 |
| 15080 | | |
| 15081 | | Matchmaker copy of fold_20240711_copi_golph3_model_0.cif H, chain H (#74) with |
| 15082 | | PredictAndBuild_7_overall_best.pdb, chain A (#78), sequence alignment score = |
| 15083 | | 2265.6 |
| 15084 | | RMSD between 256 pruned atom pairs is 1.119 angstroms; (across all 355 pairs: |
| 15085 | | 2.263) |
| 15086 | | |
| 15087 | | Matchmaker copy of fold_20240711_copi_golph3_model_0.cif H, chain H (#74) with |
| 15088 | | PredictAndBuild_7_superposed_predicted_untrimmed_cycle_1.pdb, chain A (#79), |
| 15089 | | sequence alignment score = 2735.5 |
| 15090 | | RMSD between 235 pruned atom pairs is 0.805 angstroms; (across all 372 pairs: |
| 15091 | | 5.897) |
| 15092 | | |
| 15093 | | Matchmaker copy of fold_20240711_copi_golph3_model_0.cif H, chain H (#74) with |
| 15094 | | PredictAndBuild_7_superposed_predicted_untrimmed_cycle_2.pdb, chain A (#80), |
| 15095 | | sequence alignment score = 2729.5 |
| 15096 | | RMSD between 327 pruned atom pairs is 0.888 angstroms; (across all 372 pairs: |
| 15097 | | 4.838) |
| 15098 | | |
| 15099 | | Matchmaker copy of fold_20240711_copi_golph3_model_0.cif H, chain H (#74) with |
| 15100 | | PredictAndBuild_7_overall_best_superposed_predicted_models.pdb, chain A (#77), |
| 15101 | | sequence alignment score = 2526.8 |
| 15102 | | RMSD between 255 pruned atom pairs is 0.833 angstroms; (across all 351 pairs: |
| 15103 | | 2.256) |
| 15104 | | |
| 15105 | | |
| 15106 | | > hide #!74 models |
| 15107 | | |
| 15108 | | > hide #76 models |
| 15109 | | |
| 15110 | | > hide #79 models |
| 15111 | | |
| 15112 | | > hide #80 models |
| 15113 | | |
| 15114 | | > hide #78 models |
| 15115 | | |
| 15116 | | > show #76 models |
| 15117 | | |
| 15118 | | > hide #76 models |
| 15119 | | |
| 15120 | | > show #76 models |
| 15121 | | |
| 15122 | | > hide #76 models |
| 15123 | | |
| 15124 | | > show #!74 models |
| 15125 | | |
| 15126 | | > hide #!74 models |
| 15127 | | |
| 15128 | | > show #!74 models |
| 15129 | | |
| 15130 | | > hide #!33 models |
| 15131 | | |
| 15132 | | > show #!33 models |
| 15133 | | |
| 15134 | | > show #!64 models |
| 15135 | | |
| 15136 | | > show #!66 models |
| 15137 | | |
| 15138 | | > color #77 gammav3 |
| 15139 | | |
| 15140 | | > hide #!74 models |
| 15141 | | |
| 15142 | | > show #76 models |
| 15143 | | |
| 15144 | | > hide #76 models |
| 15145 | | |
| 15146 | | > show #76 models |
| 15147 | | |
| 15148 | | > hide #!77 models |
| 15149 | | |
| 15150 | | > show #!77 models |
| 15151 | | |
| 15152 | | > hide #!77 models |
| 15153 | | |
| 15154 | | > show #!77 models |
| 15155 | | |
| 15156 | | > hide #!77 models |
| 15157 | | |
| 15158 | | > show #!77 models |
| 15159 | | |
| 15160 | | > hide #!33 models |
| 15161 | | |
| 15162 | | > hide #!64 models |
| 15163 | | |
| 15164 | | > ui mousemode right select |
| 15165 | | |
| 15166 | | Drag select of 31 residues |
| 15167 | | |
| 15168 | | > select up |
| 15169 | | |
| 15170 | | 390 atoms, 396 bonds, 49 residues, 1 model selected |
| 15171 | | |
| 15172 | | > select up |
| 15173 | | |
| 15174 | | 2085 atoms, 2126 bonds, 265 residues, 1 model selected |
| 15175 | | |
| 15176 | | > select down |
| 15177 | | |
| 15178 | | 390 atoms, 396 bonds, 49 residues, 1 model selected |
| 15179 | | |
| 15180 | | > select up |
| 15181 | | |
| 15182 | | 2085 atoms, 2126 bonds, 265 residues, 1 model selected |
| 15183 | | |
| 15184 | | > show #!33 models |
| 15185 | | |
| 15186 | | > delete sel |
| 15187 | | |
| 15188 | | > hide #!33 models |
| 15189 | | |
| 15190 | | > hide #!66 models |
| 15191 | | |
| 15192 | | > show #78 models |
| 15193 | | |
| 15194 | | > hide #78 models |
| 15195 | | |
| 15196 | | > combine #77 |
| 15197 | | |
| 15198 | | [Repeated 1 time(s)] |
| 15199 | | |
| 15200 | | > hide #!77 models |
| 15201 | | |
| 15202 | | > hide #!82 models |
| 15203 | | |
| 15204 | | > hide #76 models |
| 15205 | | |
| 15206 | | > show #76 models |
| 15207 | | |
| 15208 | | > show #78 models |
| 15209 | | |
| 15210 | | > hide #!81 models |
| 15211 | | |
| 15212 | | > show #!82 models |
| 15213 | | |
| 15214 | | > hide #!82 models |
| 15215 | | |
| 15216 | | > show #80 models |
| 15217 | | |
| 15218 | | > hide #80 models |
| 15219 | | |
| 15220 | | > hide #78 models |
| 15221 | | |
| 15222 | | > show #78 models |
| 15223 | | |
| 15224 | | > hide #78 models |
| 15225 | | |
| 15226 | | > show #79 models |
| 15227 | | |
| 15228 | | > show #78 models |
| 15229 | | |
| 15230 | | > hide #78 models |
| 15231 | | |
| 15232 | | > hide #79 models |
| 15233 | | |
| 15234 | | > show #!81 models |
| 15235 | | |
| 15236 | | Drag select of 111 residues |
| 15237 | | |
| 15238 | | > select up |
| 15239 | | |
| 15240 | | 1038 atoms, 1052 bonds, 134 residues, 1 model selected |
| 15241 | | |
| 15242 | | > delete sel |
| 15243 | | |
| 15244 | | Drag select of 57 residues, 2 pseudobonds |
| 15245 | | |
| 15246 | | > select clear |
| 15247 | | |
| 15248 | | Drag select of 53 residues, 2 pseudobonds |
| 15249 | | |
| 15250 | | > delete sel |
| 15251 | | |
| 15252 | | Drag select of 69 residues, 3 pseudobonds |
| 15253 | | |
| 15254 | | > select up |
| 15255 | | |
| 15256 | | 582 atoms, 590 bonds, 3 pseudobonds, 73 residues, 2 models selected |
| 15257 | | |
| 15258 | | > delete sel |
| 15259 | | |
| 15260 | | > fitmap #81 inMap 33.3 |
| 15261 | | |
| 15262 | | Invalid "inMap" argument: invalid density map specifier |
| 15263 | | |
| 15264 | | > fitmap #81 inMap #33.3 |
| 15265 | | |
| 15266 | | Fit molecule copy of |
| 15267 | | PredictAndBuild_7_overall_best_superposed_predicted_models.pdb (#81) to map |
| 15268 | | W20_J160_run_ct6_body003.mrc (#33.3) using 2348 atoms |
| 15269 | | average map value = 0.0134, steps = 120 |
| 15270 | | shifted from previous position = 3.48 |
| 15271 | | rotated from previous position = 4.21 degrees |
| 15272 | | atoms outside contour = 1408, contour level = 0.015167 |
| 15273 | | |
| 15274 | | Position of copy of |
| 15275 | | PredictAndBuild_7_overall_best_superposed_predicted_models.pdb (#81) relative |
| 15276 | | to W20_J160_run_ct6_body003.mrc (#33.3) coordinates: |
| 15277 | | Matrix rotation and translation |
| 15278 | | -0.20129859 -0.97948015 -0.00987448 314.24082722 |
| 15279 | | -0.86511694 0.17304913 0.47077242 170.18293950 |
| 15280 | | -0.45940347 0.10330841 -0.88219942 322.78012223 |
| 15281 | | Axis -0.62096341 0.75964188 0.19325802 |
| 15282 | | Axis point 258.89532999 0.00000000 108.65698241 |
| 15283 | | Rotation angle (degrees) 162.78955563 |
| 15284 | | Shift along axis -3.47411885 |
| 15285 | | |
| 15286 | | |
| 15287 | | > show #!33 models |
| 15288 | | |
| 15289 | | > fitmap #81 inMap #33.1 |
| 15290 | | |
| 15291 | | Fit molecule copy of |
| 15292 | | PredictAndBuild_7_overall_best_superposed_predicted_models.pdb (#81) to map |
| 15293 | | W20_J160_run_ct6_body001.mrc (#33.1) using 2348 atoms |
| 15294 | | average map value = 0.01071, steps = 112 |
| 15295 | | shifted from previous position = 3.4 |
| 15296 | | rotated from previous position = 15.1 degrees |
| 15297 | | atoms outside contour = 1585, contour level = 0.014811 |
| 15298 | | |
| 15299 | | Position of copy of |
| 15300 | | PredictAndBuild_7_overall_best_superposed_predicted_models.pdb (#81) relative |
| 15301 | | to W20_J160_run_ct6_body001.mrc (#33.1) coordinates: |
| 15302 | | Matrix rotation and translation |
| 15303 | | -0.22739783 -0.93674263 -0.26608921 334.89658150 |
| 15304 | | -0.70727752 -0.02894807 0.70634306 146.59781389 |
| 15305 | | -0.66936442 0.34881979 -0.65595428 290.22146917 |
| 15306 | | Axis -0.61036394 0.68847171 0.39174298 |
| 15307 | | Axis point 263.55345573 -0.00000000 66.31084802 |
| 15308 | | Rotation angle (degrees) 162.96971650 |
| 15309 | | Shift along axis 10.21187265 |
| 15310 | | |
| 15311 | | |
| 15312 | | > hide #!33 models |
| 15313 | | |
| 15314 | | > select add #81 |
| 15315 | | |
| 15316 | | 2348 atoms, 2383 bonds, 301 residues, 1 model selected |
| 15317 | | Drag select of 14 residues |
| 15318 | | |
| 15319 | | > select add #81 |
| 15320 | | |
| 15321 | | 2384 atoms, 2383 bonds, 310 residues, 2 models selected |
| 15322 | | |
| 15323 | | > select subtract #81 |
| 15324 | | |
| 15325 | | 36 atoms, 9 residues, 1 model selected |
| 15326 | | |
| 15327 | | > select add #81 |
| 15328 | | |
| 15329 | | 2384 atoms, 2383 bonds, 310 residues, 2 models selected |
| 15330 | | |
| 15331 | | > ui mousemode right "translate selected models" |
| 15332 | | |
| 15333 | | > view matrix models |
| 15334 | | > #76,-0.93321,0.21477,-0.28809,372.34,1.5257e-05,-0.80171,-0.59771,509.2,-0.35934,-0.5578,0.74816,166.22,#81,-0.16626,-0.95754,-0.23551,330.74,-0.84489,0.015188,0.53472,191.15,-0.50844,0.28789,-0.81155,305.14 |
| 15335 | | |
| 15336 | | > view matrix models |
| 15337 | | > #76,-0.93321,0.21477,-0.28809,369.46,1.5257e-05,-0.80171,-0.59771,509.45,-0.35934,-0.5578,0.74816,167.85,#81,-0.16626,-0.95754,-0.23551,327.86,-0.84489,0.015188,0.53472,191.4,-0.50844,0.28789,-0.81155,306.78 |
| 15338 | | |
| 15339 | | > view matrix models |
| 15340 | | > #76,-0.93321,0.21477,-0.28809,362.81,1.5257e-05,-0.80171,-0.59771,510.16,-0.35934,-0.5578,0.74816,162.77,#81,-0.16626,-0.95754,-0.23551,321.21,-0.84489,0.015188,0.53472,192.11,-0.50844,0.28789,-0.81155,301.69 |
| 15341 | | |
| 15342 | | > select add #76 |
| 15343 | | |
| 15344 | | 3188 atoms, 3221 bonds, 511 residues, 2 models selected |
| 15345 | | |
| 15346 | | > show #!33 models |
| 15347 | | |
| 15348 | | > view matrix models |
| 15349 | | > #76,-0.93321,0.21477,-0.28809,370.55,1.5257e-05,-0.80171,-0.59771,509.34,-0.35934,-0.5578,0.74816,168.09,#81,-0.16626,-0.95754,-0.23551,328.95,-0.84489,0.015188,0.53472,191.29,-0.50844,0.28789,-0.81155,307.01 |
| 15350 | | |
| 15351 | | > select subtract #81 |
| 15352 | | |
| 15353 | | 840 atoms, 838 bonds, 210 residues, 1 model selected |
| 15354 | | |
| 15355 | | > fitmap #76 inMap #33.1 |
| 15356 | | |
| 15357 | | Fit molecule 20250328_COPI_GOLPH3_model_v2.cif (#76) to map |
| 15358 | | W20_J160_run_ct6_body001.mrc (#33.1) using 840 atoms |
| 15359 | | average map value = 0.02202, steps = 60 |
| 15360 | | shifted from previous position = 2.45 |
| 15361 | | rotated from previous position = 5.43 degrees |
| 15362 | | atoms outside contour = 170, contour level = 0.014811 |
| 15363 | | |
| 15364 | | Position of 20250328_COPI_GOLPH3_model_v2.cif (#76) relative to |
| 15365 | | W20_J160_run_ct6_body001.mrc (#33.1) coordinates: |
| 15366 | | Matrix rotation and translation |
| 15367 | | -0.96070020 0.05782811 -0.27149776 408.93422016 |
| 15368 | | 0.16204598 -0.67727713 -0.71766064 466.60813032 |
| 15369 | | -0.22538019 -0.73345184 0.64128946 199.29795302 |
| 15370 | | Axis -0.13724964 -0.40083218 0.90581239 |
| 15371 | | Axis point 212.55034942 283.76140105 0.00000000 |
| 15372 | | Rotation angle (degrees) 176.70210838 |
| 15373 | | Shift along axis -62.63107629 |
| 15374 | | |
| 15375 | | |
| 15376 | | > fitmap #81 inMap #33.1 |
| 15377 | | |
| 15378 | | Fit molecule copy of |
| 15379 | | PredictAndBuild_7_overall_best_superposed_predicted_models.pdb (#81) to map |
| 15380 | | W20_J160_run_ct6_body001.mrc (#33.1) using 2348 atoms |
| 15381 | | average map value = 0.01071, steps = 56 |
| 15382 | | shifted from previous position = 1.86 |
| 15383 | | rotated from previous position = 0.0322 degrees |
| 15384 | | atoms outside contour = 1584, contour level = 0.014811 |
| 15385 | | |
| 15386 | | Position of copy of |
| 15387 | | PredictAndBuild_7_overall_best_superposed_predicted_models.pdb (#81) relative |
| 15388 | | to W20_J160_run_ct6_body001.mrc (#33.1) coordinates: |
| 15389 | | Matrix rotation and translation |
| 15390 | | -0.22745563 -0.93688214 -0.26554811 334.85796779 |
| 15391 | | -0.70718029 -0.02854799 0.70645669 146.50571825 |
| 15392 | | -0.66944752 0.34847794 -0.65605118 290.29252721 |
| 15393 | | Axis -0.61030750 0.68859628 0.39161196 |
| 15394 | | Axis point 263.52285147 0.00000000 66.35287841 |
| 15395 | | Rotation angle (degrees) 162.94573059 |
| 15396 | | Shift along axis 10.19898967 |
| 15397 | | |
| 15398 | | |
| 15399 | | > hide #!81 models |
| 15400 | | |
| 15401 | | > hide #76 models |
| 15402 | | |
| 15403 | | > show #!81 models |
| 15404 | | |
| 15405 | | > hide #!33 models |
| 15406 | | |
| 15407 | | > view matrix models |
| 15408 | | > #76,-0.94628,0.12198,-0.29946,442.05,0.074145,-0.8196,-0.56813,481.51,-0.31474,-0.55981,0.76652,186.98 |
| 15409 | | |
| 15410 | | > ui mousemode right select |
| 15411 | | |
| 15412 | | Drag select of 123 residues |
| 15413 | | |
| 15414 | | > select up |
| 15415 | | |
| 15416 | | 983 atoms, 995 bonds, 129 residues, 1 model selected |
| 15417 | | |
| 15418 | | > delete sel |
| 15419 | | |
| 15420 | | > show #!33 models |
| 15421 | | |
| 15422 | | > fitmap #81 inMap #33.1 |
| 15423 | | |
| 15424 | | Fit molecule copy of |
| 15425 | | PredictAndBuild_7_overall_best_superposed_predicted_models.pdb (#81) to map |
| 15426 | | W20_J160_run_ct6_body001.mrc (#33.1) using 1365 atoms |
| 15427 | | average map value = 0.01415, steps = 116 |
| 15428 | | shifted from previous position = 2.3 |
| 15429 | | rotated from previous position = 16.3 degrees |
| 15430 | | atoms outside contour = 753, contour level = 0.014811 |
| 15431 | | |
| 15432 | | Position of copy of |
| 15433 | | PredictAndBuild_7_overall_best_superposed_predicted_models.pdb (#81) relative |
| 15434 | | to W20_J160_run_ct6_body001.mrc (#33.1) coordinates: |
| 15435 | | Matrix rotation and translation |
| 15436 | | 0.01747338 -0.99098181 -0.13285229 281.52273337 |
| 15437 | | -0.71306863 -0.10549261 0.69311214 162.28311421 |
| 15438 | | -0.70087645 0.08262179 -0.70848136 343.51081858 |
| 15439 | | Axis -0.69455315 0.64623949 0.31618104 |
| 15440 | | Axis point 0.00000000 268.41792558 210.72025848 |
| 15441 | | Rotation angle (degrees) 153.92902085 |
| 15442 | | Shift along axis 17.95286420 |
| 15443 | | |
| 15444 | | |
| 15445 | | > show #!74 models |
| 15446 | | |
| 15447 | | > hide #!74 models |
| 15448 | | |
| 15449 | | > show #!75 models |
| 15450 | | |
| 15451 | | > hide #!75 models |
| 15452 | | |
| 15453 | | > show #76 models |
| 15454 | | |
| 15455 | | > select add #76 |
| 15456 | | |
| 15457 | | 840 atoms, 838 bonds, 210 residues, 1 model selected |
| 15458 | | Drag select of 33.1 W20_J160_run_ct6_body001.mrc , 2 residues |
| 15459 | | |
| 15460 | | > select clear |
| 15461 | | |
| 15462 | | > select add #76 |
| 15463 | | |
| 15464 | | 840 atoms, 838 bonds, 210 residues, 1 model selected |
| 15465 | | Drag select of 33.1 W20_J160_run_ct6_body001.mrc , 5 residues |
| 15466 | | |
| 15467 | | > select add #76 |
| 15468 | | |
| 15469 | | 840 atoms, 838 bonds, 210 residues, 3 models selected |
| 15470 | | |
| 15471 | | > select subtract #76 |
| 15472 | | |
| 15473 | | 2 models selected |
| 15474 | | |
| 15475 | | > select clear |
| 15476 | | |
| 15477 | | > select add #76 |
| 15478 | | |
| 15479 | | 840 atoms, 838 bonds, 210 residues, 1 model selected |
| 15480 | | |
| 15481 | | > ui mousemode right "translate selected models" |
| 15482 | | |
| 15483 | | > view matrix models |
| 15484 | | > #76,-0.94628,0.12198,-0.29946,406.27,0.074145,-0.8196,-0.56813,495.91,-0.31474,-0.55981,0.76652,151.84 |
| 15485 | | |
| 15486 | | > fitmap #76 inMap #33.1 |
| 15487 | | |
| 15488 | | Fit molecule 20250328_COPI_GOLPH3_model_v2.cif (#76) to map |
| 15489 | | W20_J160_run_ct6_body001.mrc (#33.1) using 840 atoms |
| 15490 | | average map value = 0.02201, steps = 112 |
| 15491 | | shifted from previous position = 10.6 |
| 15492 | | rotated from previous position = 0.0236 degrees |
| 15493 | | atoms outside contour = 169, contour level = 0.014811 |
| 15494 | | |
| 15495 | | Position of 20250328_COPI_GOLPH3_model_v2.cif (#76) relative to |
| 15496 | | W20_J160_run_ct6_body001.mrc (#33.1) coordinates: |
| 15497 | | Matrix rotation and translation |
| 15498 | | -0.96080554 0.05790187 -0.27110898 408.84449350 |
| 15499 | | 0.16172293 -0.67722645 -0.71778133 466.70494589 |
| 15500 | | -0.22516305 -0.73349282 0.64131886 199.25734435 |
| 15501 | | Axis -0.13708008 -0.40087044 0.90582114 |
| 15502 | | Axis point 212.50907521 283.77894704 0.00000000 |
| 15503 | | Rotation angle (degrees) 176.71471075 |
| 15504 | | Shift along axis -62.64113752 |
| 15505 | | |
| 15506 | | |
| 15507 | | > select subtract #76 |
| 15508 | | |
| 15509 | | Nothing selected |
| 15510 | | |
| 15511 | | > hide #76 models |
| 15512 | | |
| 15513 | | > hide #!33 models |
| 15514 | | |
| 15515 | | > ui mousemode right select |
| 15516 | | |
| 15517 | | Drag select of 66 residues |
| 15518 | | |
| 15519 | | > select up |
| 15520 | | |
| 15521 | | 1365 atoms, 1387 bonds, 172 residues, 1 model selected |
| 15522 | | |
| 15523 | | > select down |
| 15524 | | |
| 15525 | | 533 atoms, 66 residues, 1 model selected |
| 15526 | | |
| 15527 | | > delete sel |
| 15528 | | |
| 15529 | | > fitmap #81 inMap #33.1 |
| 15530 | | |
| 15531 | | Fit molecule copy of |
| 15532 | | PredictAndBuild_7_overall_best_superposed_predicted_models.pdb (#81) to map |
| 15533 | | W20_J160_run_ct6_body001.mrc (#33.1) using 832 atoms |
| 15534 | | average map value = 0.01316, steps = 84 |
| 15535 | | shifted from previous position = 3.31 |
| 15536 | | rotated from previous position = 11.8 degrees |
| 15537 | | atoms outside contour = 498, contour level = 0.014811 |
| 15538 | | |
| 15539 | | Position of copy of |
| 15540 | | PredictAndBuild_7_overall_best_superposed_predicted_models.pdb (#81) relative |
| 15541 | | to W20_J160_run_ct6_body001.mrc (#33.1) coordinates: |
| 15542 | | Matrix rotation and translation |
| 15543 | | 0.14691503 -0.96385323 -0.22226771 264.04905282 |
| 15544 | | -0.78499351 -0.25032946 0.56667482 218.34222261 |
| 15545 | | -0.60183151 0.09122566 -0.79339568 335.48317698 |
| 15546 | | Axis -0.74977491 0.59856539 0.28205861 |
| 15547 | | Axis point 0.00000000 250.13903908 195.48982431 |
| 15548 | | Rotation angle (degrees) 161.51470465 |
| 15549 | | Shift along axis 27.34066185 |
| 15550 | | |
| 15551 | | |
| 15552 | | > show #!33 models |
| 15553 | | |
| 15554 | | > show #76 models |
| 15555 | | |
| 15556 | | > hide #!33 models |
| 15557 | | |
| 15558 | | > hide #76 models |
| 15559 | | |
| 15560 | | Drag select of 75 residues |
| 15561 | | |
| 15562 | | > delete sel |
| 15563 | | |
| 15564 | | > fitmap #81 inMap #33.1 |
| 15565 | | |
| 15566 | | Fit molecule copy of |
| 15567 | | PredictAndBuild_7_overall_best_superposed_predicted_models.pdb (#81) to map |
| 15568 | | W20_J160_run_ct6_body001.mrc (#33.1) using 239 atoms |
| 15569 | | average map value = 0.01661, steps = 68 |
| 15570 | | shifted from previous position = 0.894 |
| 15571 | | rotated from previous position = 22.1 degrees |
| 15572 | | atoms outside contour = 87, contour level = 0.014811 |
| 15573 | | |
| 15574 | | Position of copy of |
| 15575 | | PredictAndBuild_7_overall_best_superposed_predicted_models.pdb (#81) relative |
| 15576 | | to W20_J160_run_ct6_body001.mrc (#33.1) coordinates: |
| 15577 | | Matrix rotation and translation |
| 15578 | | 0.01560880 -0.84592101 -0.53307975 307.86268532 |
| 15579 | | -0.85667939 -0.28624403 0.42914424 255.66225987 |
| 15580 | | -0.51561303 0.44998001 -0.72915101 245.26898996 |
| 15581 | | Axis 0.71258549 -0.59736389 -0.36793789 |
| 15582 | | Axis point 0.00000000 255.65895561 203.35484992 |
| 15583 | | Rotation angle (degrees) 179.16231543 |
| 15584 | | Shift along axis -23.58867527 |
| 15585 | | |
| 15586 | | |
| 15587 | | > show #76 models |
| 15588 | | |
| 15589 | | > show #!33 models |
| 15590 | | |
| 15591 | | > hide #!33 models |
| 15592 | | |
| 15593 | | > show #!82 models |
| 15594 | | |
| 15595 | | > hide #76 models |
| 15596 | | |
| 15597 | | > hide #!81 models |
| 15598 | | |
| 15599 | | Drag select of 9 residues |
| 15600 | | |
| 15601 | | > select add #82 |
| 15602 | | |
| 15603 | | 4394 atoms, 4464 bonds, 1 pseudobond, 561 residues, 2 models selected |
| 15604 | | |
| 15605 | | > select subtract #82 |
| 15606 | | |
| 15607 | | Nothing selected |
| 15608 | | |
| 15609 | | > show #!81 models |
| 15610 | | |
| 15611 | | > hide #!82 models |
| 15612 | | |
| 15613 | | > hide #!81 models |
| 15614 | | |
| 15615 | | > show #!74 models |
| 15616 | | |
| 15617 | | > hide #!74 models |
| 15618 | | |
| 15619 | | > show #76 models |
| 15620 | | |
| 15621 | | > show #!77 models |
| 15622 | | |
| 15623 | | > hide #!77 models |
| 15624 | | |
| 15625 | | > show #78 models |
| 15626 | | |
| 15627 | | > show #!33 models |
| 15628 | | |
| 15629 | | > combine #78 |
| 15630 | | |
| 15631 | | > hide #78 models |
| 15632 | | |
| 15633 | | > hide #!33 models |
| 15634 | | |
| 15635 | | > hide #76 models |
| 15636 | | |
| 15637 | | > show #76 models |
| 15638 | | |
| 15639 | | > hide #76 models |
| 15640 | | |
| 15641 | | > show #76 models |
| 15642 | | |
| 15643 | | > hide #76 models |
| 15644 | | |
| 15645 | | Drag select of 49 residues |
| 15646 | | |
| 15647 | | > select up |
| 15648 | | |
| 15649 | | 441 atoms, 448 bonds, 56 residues, 1 model selected |
| 15650 | | |
| 15651 | | > delete sel |
| 15652 | | |
| 15653 | | > show #!74 models |
| 15654 | | |
| 15655 | | > hide #!74 models |
| 15656 | | |
| 15657 | | > show #76 models |
| 15658 | | |
| 15659 | | > fitmap #83 inMap #33.3 |
| 15660 | | |
| 15661 | | Fit molecule copy of PredictAndBuild_7_overall_best.pdb (#83) to map |
| 15662 | | W20_J160_run_ct6_body003.mrc (#33.3) using 3367 atoms |
| 15663 | | average map value = 0.01915, steps = 88 |
| 15664 | | shifted from previous position = 1.31 |
| 15665 | | rotated from previous position = 3.07 degrees |
| 15666 | | atoms outside contour = 1342, contour level = 0.015167 |
| 15667 | | |
| 15668 | | Position of copy of PredictAndBuild_7_overall_best.pdb (#83) relative to |
| 15669 | | W20_J160_run_ct6_body003.mrc (#33.3) coordinates: |
| 15670 | | Matrix rotation and translation |
| 15671 | | -0.19901990 -0.97974509 -0.02215055 315.72725675 |
| 15672 | | -0.86258557 0.16440368 0.47845331 170.01428108 |
| 15673 | | -0.46512065 0.11432847 -0.87783357 321.74102683 |
| 15674 | | Axis -0.62215321 0.75687028 0.20018181 |
| 15675 | | Axis point 259.60135583 0.00000000 107.39223084 |
| 15676 | | Rotation angle (degrees) 162.98435754 |
| 15677 | | Shift along axis -3.34526606 |
| 15678 | | |
| 15679 | | |
| 15680 | | > show #!33 models |
| 15681 | | |
| 15682 | | > save "/Users/becca/Desktop/Postdoc/COPI notebooks and |
| 15683 | | > resources/Processing/W20_MM_cyto_COPI/20250705_W20_COPI_cyto_alignments.cxs" |
| 15684 | | |
| 15685 | | > hide #!33 models |
| 15686 | | |
| 15687 | | > show #!9 models |
| 15688 | | |
| 15689 | | > hide #!9 models |
| 15690 | | |
| 15691 | | > show #!45 models |
| 15692 | | |
| 15693 | | > show #!46 models |
| 15694 | | |
| 15695 | | > hide #!46 models |
| 15696 | | |
| 15697 | | > hide #!45 models |
| 15698 | | |
| 15699 | | > show #!23 models |
| 15700 | | |
| 15701 | | > hide #!23 models |
| 15702 | | |
| 15703 | | > show #!25 models |
| 15704 | | |
| 15705 | | > fitmap #76 inMap #25 |
| 15706 | | |
| 15707 | | Fit molecule 20250328_COPI_GOLPH3_model_v2.cif (#76) to map |
| 15708 | | W20_J4027_005_volume_map_gammazeta_local.mrc (#25) using 840 atoms |
| 15709 | | average map value = 0.4915, steps = 56 |
| 15710 | | shifted from previous position = 1.01 |
| 15711 | | rotated from previous position = 4.93 degrees |
| 15712 | | atoms outside contour = 19, contour level = 0.2532 |
| 15713 | | |
| 15714 | | Position of 20250328_COPI_GOLPH3_model_v2.cif (#76) relative to |
| 15715 | | W20_J4027_005_volume_map_gammazeta_local.mrc (#25) coordinates: |
| 15716 | | Matrix rotation and translation |
| 15717 | | -0.95361160 0.05892580 -0.29521631 421.68865993 |
| 15718 | | 0.13475176 -0.79337176 -0.59363560 471.58547391 |
| 15719 | | -0.26919673 -0.60587871 0.74862815 156.24096699 |
| 15720 | | Axis -0.15097143 -0.32085083 0.93501999 |
| 15721 | | Axis point 218.58287062 267.06046758 0.00000000 |
| 15722 | | Rotation angle (degrees) 177.67614662 |
| 15723 | | Shift along axis -68.88310337 |
| 15724 | | |
| 15725 | | |
| 15726 | | > fitmap #77 inMap #25 |
| 15727 | | |
| 15728 | | Fit molecule PredictAndBuild_7_overall_best_superposed_predicted_models.pdb |
| 15729 | | (#77) to map W20_J4027_005_volume_map_gammazeta_local.mrc (#25) using 4394 |
| 15730 | | atoms |
| 15731 | | average map value = 0.2705, steps = 120 |
| 15732 | | shifted from previous position = 6.58 |
| 15733 | | rotated from previous position = 13.2 degrees |
| 15734 | | atoms outside contour = 2142, contour level = 0.2532 |
| 15735 | | |
| 15736 | | Position of PredictAndBuild_7_overall_best_superposed_predicted_models.pdb |
| 15737 | | (#77) relative to W20_J4027_005_volume_map_gammazeta_local.mrc (#25) |
| 15738 | | coordinates: |
| 15739 | | Matrix rotation and translation |
| 15740 | | -0.08911154 -0.97740547 -0.19167078 312.61104116 |
| 15741 | | -0.86513544 -0.01940313 0.50116283 207.38914458 |
| 15742 | | -0.49355830 0.21048058 -0.84385907 317.01597391 |
| 15743 | | Axis -0.66998680 0.69581356 0.25876858 |
| 15744 | | Axis point 280.81289084 0.00000000 94.39286792 |
| 15745 | | Rotation angle (degrees) 167.47114130 |
| 15746 | | Shift along axis 16.89268056 |
| 15747 | | |
| 15748 | | |
| 15749 | | > fitmap #78 inMap #25 |
| 15750 | | |
| 15751 | | Fit molecule PredictAndBuild_7_overall_best.pdb (#78) to map |
| 15752 | | W20_J4027_005_volume_map_gammazeta_local.mrc (#25) using 3808 atoms |
| 15753 | | average map value = 0.2752, steps = 92 |
| 15754 | | shifted from previous position = 5.3 |
| 15755 | | rotated from previous position = 5.46 degrees |
| 15756 | | atoms outside contour = 1850, contour level = 0.2532 |
| 15757 | | |
| 15758 | | Position of PredictAndBuild_7_overall_best.pdb (#78) relative to |
| 15759 | | W20_J4027_005_volume_map_gammazeta_local.mrc (#25) coordinates: |
| 15760 | | Matrix rotation and translation |
| 15761 | | -0.22705266 -0.96732791 -0.11279981 326.50448848 |
| 15762 | | -0.84045401 0.13610962 0.52451047 175.87350921 |
| 15763 | | -0.49202048 0.21389455 -0.84389867 316.29589561 |
| 15764 | | Axis -0.61344445 0.74893397 0.25056699 |
| 15765 | | Axis point 262.22373167 0.00000000 100.11436660 |
| 15766 | | Rotation angle (degrees) 165.33460884 |
| 15767 | | Shift along axis 10.67858762 |
| 15768 | | |
| 15769 | | |
| 15770 | | > fitmap #79 inMap #25 |
| 15771 | | |
| 15772 | | Fit molecule PredictAndBuild_7_superposed_predicted_untrimmed_cycle_1.pdb |
| 15773 | | (#79) to map W20_J4027_005_volume_map_gammazeta_local.mrc (#25) using 6818 |
| 15774 | | atoms |
| 15775 | | average map value = 0.2107, steps = 172 |
| 15776 | | shifted from previous position = 11.4 |
| 15777 | | rotated from previous position = 5.55 degrees |
| 15778 | | atoms outside contour = 4196, contour level = 0.2532 |
| 15779 | | |
| 15780 | | Position of PredictAndBuild_7_superposed_predicted_untrimmed_cycle_1.pdb (#79) |
| 15781 | | relative to W20_J4027_005_volume_map_gammazeta_local.mrc (#25) coordinates: |
| 15782 | | Matrix rotation and translation |
| 15783 | | -0.68234026 -0.66974665 -0.29300373 396.66689712 |
| 15784 | | -0.70556204 0.49846977 0.50369643 102.82972208 |
| 15785 | | -0.19129550 0.55042467 -0.81267381 199.71422066 |
| 15786 | | Axis 0.39762109 -0.86545829 -0.30476124 |
| 15787 | | Axis point 218.52168169 0.00000000 88.43752525 |
| 15788 | | Rotation angle (degrees) 176.63137373 |
| 15789 | | Shift along axis 7.86313588 |
| 15790 | | |
| 15791 | | |
| 15792 | | > fitmap #80 inMap #25 |
| 15793 | | |
| 15794 | | Fit molecule PredictAndBuild_7_superposed_predicted_untrimmed_cycle_2.pdb |
| 15795 | | (#80) to map W20_J4027_005_volume_map_gammazeta_local.mrc (#25) using 6818 |
| 15796 | | atoms |
| 15797 | | average map value = 0.2002, steps = 128 |
| 15798 | | shifted from previous position = 3.93 |
| 15799 | | rotated from previous position = 13.2 degrees |
| 15800 | | atoms outside contour = 4363, contour level = 0.2532 |
| 15801 | | |
| 15802 | | Position of PredictAndBuild_7_superposed_predicted_untrimmed_cycle_2.pdb (#80) |
| 15803 | | relative to W20_J4027_005_volume_map_gammazeta_local.mrc (#25) coordinates: |
| 15804 | | Matrix rotation and translation |
| 15805 | | -0.61808214 -0.74524170 -0.25017850 390.14177001 |
| 15806 | | -0.76859940 0.50608871 0.39131723 125.96407520 |
| 15807 | | -0.16501341 0.43415324 -0.88559671 224.77237006 |
| 15808 | | Axis 0.43643009 -0.86769550 -0.23797752 |
| 15809 | | Axis point 223.54405179 0.00000000 100.58607108 |
| 15810 | | Rotation angle (degrees) 177.18705406 |
| 15811 | | Shift along axis 7.48037378 |
| 15812 | | |
| 15813 | | |
| 15814 | | > fitmap #81 inMap #25 |
| 15815 | | |
| 15816 | | Fit molecule copy of |
| 15817 | | PredictAndBuild_7_overall_best_superposed_predicted_models.pdb (#81) to map |
| 15818 | | W20_J4027_005_volume_map_gammazeta_local.mrc (#25) using 239 atoms |
| 15819 | | average map value = 0.2847, steps = 56 |
| 15820 | | shifted from previous position = 2.17 |
| 15821 | | rotated from previous position = 9.62 degrees |
| 15822 | | atoms outside contour = 73, contour level = 0.2532 |
| 15823 | | |
| 15824 | | Position of copy of |
| 15825 | | PredictAndBuild_7_overall_best_superposed_predicted_models.pdb (#81) relative |
| 15826 | | to W20_J4027_005_volume_map_gammazeta_local.mrc (#25) coordinates: |
| 15827 | | Matrix rotation and translation |
| 15828 | | -0.00169329 -0.79343761 -0.60864924 319.68781006 |
| 15829 | | -0.93882519 -0.20835191 0.27422025 280.49121917 |
| 15830 | | -0.34438989 0.57187957 -0.74454641 207.08413091 |
| 15831 | | Axis 0.70242609 -0.62360775 -0.34309032 |
| 15832 | | Axis point 0.00000000 266.91836814 201.99199878 |
| 15833 | | Rotation angle (degrees) 167.76748361 |
| 15834 | | Shift along axis -21.40799989 |
| 15835 | | |
| 15836 | | |
| 15837 | | > fitmap #82 inMap #25 |
| 15838 | | |
| 15839 | | Fit molecule copy of |
| 15840 | | PredictAndBuild_7_overall_best_superposed_predicted_models.pdb (#82) to map |
| 15841 | | W20_J4027_005_volume_map_gammazeta_local.mrc (#25) using 4394 atoms |
| 15842 | | average map value = 0.2705, steps = 120 |
| 15843 | | shifted from previous position = 6.58 |
| 15844 | | rotated from previous position = 13.2 degrees |
| 15845 | | atoms outside contour = 2142, contour level = 0.2532 |
| 15846 | | |
| 15847 | | Position of copy of |
| 15848 | | PredictAndBuild_7_overall_best_superposed_predicted_models.pdb (#82) relative |
| 15849 | | to W20_J4027_005_volume_map_gammazeta_local.mrc (#25) coordinates: |
| 15850 | | Matrix rotation and translation |
| 15851 | | -0.08911154 -0.97740547 -0.19167078 312.61104116 |
| 15852 | | -0.86513544 -0.01940313 0.50116283 207.38914458 |
| 15853 | | -0.49355830 0.21048058 -0.84385907 317.01597391 |
| 15854 | | Axis -0.66998680 0.69581356 0.25876858 |
| 15855 | | Axis point 280.81289084 0.00000000 94.39286792 |
| 15856 | | Rotation angle (degrees) 167.47114130 |
| 15857 | | Shift along axis 16.89268056 |
| 15858 | | |
| 15859 | | |
| 15860 | | > fitmap #83 inMap #25 |
| 15861 | | |
| 15862 | | Fit molecule copy of PredictAndBuild_7_overall_best.pdb (#83) to map |
| 15863 | | W20_J4027_005_volume_map_gammazeta_local.mrc (#25) using 3367 atoms |
| 15864 | | average map value = 0.2736, steps = 104 |
| 15865 | | shifted from previous position = 4.19 |
| 15866 | | rotated from previous position = 7.76 degrees |
| 15867 | | atoms outside contour = 1660, contour level = 0.2532 |
| 15868 | | |
| 15869 | | Position of copy of PredictAndBuild_7_overall_best.pdb (#83) relative to |
| 15870 | | W20_J4027_005_volume_map_gammazeta_local.mrc (#25) coordinates: |
| 15871 | | Matrix rotation and translation |
| 15872 | | -0.20610544 -0.97397844 -0.09426847 320.31886481 |
| 15873 | | -0.84612639 0.12899838 0.51713592 179.53786545 |
| 15874 | | -0.49151875 0.18634757 -0.85069613 321.39018439 |
| 15875 | | Axis -0.62117839 0.74598543 0.24008987 |
| 15876 | | Axis point 263.85010261 0.00000000 102.01189567 |
| 15877 | | Rotation angle (degrees) 164.55823923 |
| 15878 | | Shift along axis 12.12000484 |
| 15879 | | |
| 15880 | | |
| 15881 | | > hide #83 models |
| 15882 | | |
| 15883 | | > hide #76 models |
| 15884 | | |
| 15885 | | > open |
| 15886 | | > /Users/becca/Desktop/Postdoc/Phenix/W20_MM_cyto_COPI/PredictAndBuildCryoEM_4/PredictAndBuild_4_overall_best_superposed_predicted_models.pdb |
| 15887 | | |
| 15888 | | Chain information for |
| 15889 | | PredictAndBuild_4_overall_best_superposed_predicted_models.pdb #84 |
| 15890 | | --- |
| 15891 | | Chain | Description |
| 15892 | | A | No description available |
| 15893 | | |
| 15894 | | |
| 15895 | | > open |
| 15896 | | > /Users/becca/Desktop/Postdoc/Phenix/W20_MM_cyto_COPI/PredictAndBuildCryoEM_4/PredictAndBuild_4_overall_best.pdb |
| 15897 | | |
| 15898 | | Chain information for PredictAndBuild_4_overall_best.pdb #85 |
| 15899 | | --- |
| 15900 | | Chain | Description |
| 15901 | | A | No description available |
| 15902 | | |
| 15903 | | |
| 15904 | | > open |
| 15905 | | > /Users/becca/Desktop/Postdoc/Phenix/W20_MM_cyto_COPI/PredictAndBuildCryoEM_4/PredictAndBuild_4_superposed_predicted_untrimmed_cycle_1.pdb |
| 15906 | | |
| 15907 | | Chain information for |
| 15908 | | PredictAndBuild_4_superposed_predicted_untrimmed_cycle_1.pdb #86 |
| 15909 | | --- |
| 15910 | | Chain | Description |
| 15911 | | A | No description available |
| 15912 | | |
| 15913 | | |
| 15914 | | > open |
| 15915 | | > /Users/becca/Desktop/Postdoc/Phenix/W20_MM_cyto_COPI/PredictAndBuildCryoEM_4/PredictAndBuild_4_superposed_predicted_untrimmed_cycle_2.pdb |
| 15916 | | |
| 15917 | | Chain information for |
| 15918 | | PredictAndBuild_4_superposed_predicted_untrimmed_cycle_2.pdb #87 |
| 15919 | | --- |
| 15920 | | Chain | Description |
| 15921 | | A | No description available |
| 15922 | | |
| 15923 | | |
| 15924 | | > open |
| 15925 | | > /Users/becca/Desktop/Postdoc/Phenix/W20_MM_cyto_COPI/PredictAndBuildCryoEM_4/PredictAndBuild_4_superposed_predicted_untrimmed_cycle_3.pdb |
| 15926 | | |
| 15927 | | Chain information for |
| 15928 | | PredictAndBuild_4_superposed_predicted_untrimmed_cycle_3.pdb #88 |
| 15929 | | --- |
| 15930 | | Chain | Description |
| 15931 | | A | No description available |
| 15932 | | |
| 15933 | | Computing secondary structure |
| 15934 | | [Repeated 4 time(s)] |
| 15935 | | |
| 15936 | | > ui tool show Matchmaker |
| 15937 | | |
| 15938 | | > matchmaker #86-88#!84-85 to #83 |
| 15939 | | |
| 15940 | | Parameters |
| 15941 | | --- |
| 15942 | | Chain pairing | bb |
| 15943 | | Alignment algorithm | Needleman-Wunsch |
| 15944 | | Similarity matrix | BLOSUM-62 |
| 15945 | | SS fraction | 0.3 |
| 15946 | | Gap open (HH/SS/other) | 18/18/6 |
| 15947 | | Gap extend | 1 |
| 15948 | | SS matrix | | | H | S | O |
| 15949 | | ---|---|---|--- |
| 15950 | | H | 6 | -9 | -6 |
| 15951 | | S | | 6 | -6 |
| 15952 | | O | | | 4 |
| 15953 | | Iteration cutoff | 2 |
| 15954 | | |
| 15955 | | Matchmaker copy of PredictAndBuild_7_overall_best.pdb, chain A (#83) with |
| 15956 | | PredictAndBuild_4_superposed_predicted_untrimmed_cycle_1.pdb, chain A (#86), |
| 15957 | | sequence alignment score = 2398.8 |
| 15958 | | RMSD between 245 pruned atom pairs is 0.859 angstroms; (across all 431 pairs: |
| 15959 | | 6.247) |
| 15960 | | |
| 15961 | | Matchmaker copy of PredictAndBuild_7_overall_best.pdb, chain A (#83) with |
| 15962 | | PredictAndBuild_4_superposed_predicted_untrimmed_cycle_2.pdb, chain A (#87), |
| 15963 | | sequence alignment score = 2398.8 |
| 15964 | | RMSD between 253 pruned atom pairs is 0.822 angstroms; (across all 431 pairs: |
| 15965 | | 5.398) |
| 15966 | | |
| 15967 | | Matchmaker copy of PredictAndBuild_7_overall_best.pdb, chain A (#83) with |
| 15968 | | PredictAndBuild_4_superposed_predicted_untrimmed_cycle_3.pdb, chain A (#88), |
| 15969 | | sequence alignment score = 2398.8 |
| 15970 | | RMSD between 255 pruned atom pairs is 0.834 angstroms; (across all 431 pairs: |
| 15971 | | 5.572) |
| 15972 | | |
| 15973 | | Matchmaker copy of PredictAndBuild_7_overall_best.pdb, chain A (#83) with |
| 15974 | | PredictAndBuild_4_overall_best_superposed_predicted_models.pdb, chain A (#84), |
| 15975 | | sequence alignment score = 2353 |
| 15976 | | RMSD between 261 pruned atom pairs is 0.933 angstroms; (across all 426 pairs: |
| 15977 | | 5.270) |
| 15978 | | |
| 15979 | | Matchmaker copy of PredictAndBuild_7_overall_best.pdb, chain A (#83) with |
| 15980 | | PredictAndBuild_4_overall_best.pdb, chain A (#85), sequence alignment score = |
| 15981 | | 2185.7 |
| 15982 | | RMSD between 274 pruned atom pairs is 0.698 angstroms; (across all 415 pairs: |
| 15983 | | 4.042) |
| 15984 | | |
| 15985 | | |
| 15986 | | > open "/Users/becca/Desktop/Postdoc/COPI notebooks and resources/Alphafold |
| 15987 | | > predictions/fold_20250704_copi_abbprimedgz_trucadg/fold_20250704_copi_abbprimedgz_trucadg_model_0.cif" |
| 15988 | | |
| 15989 | | Chain information for fold_20250704_copi_abbprimedgz_trucadg_model_0.cif #89 |
| 15990 | | --- |
| 15991 | | Chain | Description |
| 15992 | | A | . |
| 15993 | | B | . |
| 15994 | | C | . |
| 15995 | | D | . |
| 15996 | | E | . |
| 15997 | | F | . |
| 15998 | | |
| 15999 | | |
| 16000 | | > open "/Users/becca/Desktop/Postdoc/COPI notebooks and resources/Alphafold |
| 16001 | | > predictions/fold_20250704_copi_abbprimedgz_trucadg/fold_20250704_copi_abbprimedgz_trucadg_model_1.cif" |
| 16002 | | |
| 16003 | | Chain information for fold_20250704_copi_abbprimedgz_trucadg_model_1.cif #90 |
| 16004 | | --- |
| 16005 | | Chain | Description |
| 16006 | | A | . |
| 16007 | | B | . |
| 16008 | | C | . |
| 16009 | | D | . |
| 16010 | | E | . |
| 16011 | | F | . |
| 16012 | | |
| 16013 | | |
| 16014 | | > open "/Users/becca/Desktop/Postdoc/COPI notebooks and resources/Alphafold |
| 16015 | | > predictions/fold_20250704_copi_abbprimedgz_trucadg/fold_20250704_copi_abbprimedgz_trucadg_model_2.cif" |
| 16016 | | |
| 16017 | | Chain information for fold_20250704_copi_abbprimedgz_trucadg_model_2.cif #91 |
| 16018 | | --- |
| 16019 | | Chain | Description |
| 16020 | | A | . |
| 16021 | | B | . |
| 16022 | | C | . |
| 16023 | | D | . |
| 16024 | | E | . |
| 16025 | | F | . |
| 16026 | | |
| 16027 | | |
| 16028 | | > open "/Users/becca/Desktop/Postdoc/COPI notebooks and resources/Alphafold |
| 16029 | | > predictions/fold_20250704_copi_abbprimedgz_trucadg/fold_20250704_copi_abbprimedgz_trucadg_model_3.cif" |
| 16030 | | |
| 16031 | | Chain information for fold_20250704_copi_abbprimedgz_trucadg_model_3.cif #92 |
| 16032 | | --- |
| 16033 | | Chain | Description |
| 16034 | | A | . |
| 16035 | | B | . |
| 16036 | | C | . |
| 16037 | | D | . |
| 16038 | | E | . |
| 16039 | | F | . |
| 16040 | | |
| 16041 | | |
| 16042 | | > open "/Users/becca/Desktop/Postdoc/COPI notebooks and resources/Alphafold |
| 16043 | | > predictions/fold_20250704_copi_abbprimedgz_trucadg/fold_20250704_copi_abbprimedgz_trucadg_model_4.cif" |
| 16044 | | |
| 16045 | | Chain information for fold_20250704_copi_abbprimedgz_trucadg_model_4.cif #93 |
| 16046 | | --- |
| 16047 | | Chain | Description |
| 16048 | | A | . |
| 16049 | | B | . |
| 16050 | | C | . |
| 16051 | | D | . |
| 16052 | | E | . |
| 16053 | | F | . |
| 16054 | | |
| 16055 | | |
| 16056 | | > open "/Users/becca/Desktop/Postdoc/COPI notebooks and resources/Structure |
| 16057 | | > files/COPI_soluble_job160_dhelix.pdb" |
| 16058 | | |
| 16059 | | Chain information for COPI_soluble_job160_dhelix.pdb #94 |
| 16060 | | --- |
| 16061 | | Chain | Description |
| 16062 | | D | No description available |
| 16063 | | E | No description available |
| 16064 | | F | No description available |
| 16065 | | G | No description available |
| 16066 | | H | No description available |
| 16067 | | I | No description available |
| 16068 | | J | No description available |
| 16069 | | K | No description available |
| 16070 | | L | No description available |
| 16071 | | M | No description available |
| 16072 | | N | No description available |
| 16073 | | O | No description available |
| 16074 | | P | No description available |
| 16075 | | Q | No description available |
| 16076 | | |
| 16077 | | |
| 16078 | | > open "/Users/becca/Desktop/Postdoc/COPI notebooks and resources/Structure |
| 16079 | | > files/COPI_soluble_job155_dhelix.pdb" |
| 16080 | | |
| 16081 | | Chain information for COPI_soluble_job155_dhelix.pdb #95 |
| 16082 | | --- |
| 16083 | | Chain | Description |
| 16084 | | D | No description available |
| 16085 | | E | No description available |
| 16086 | | F | No description available |
| 16087 | | G | No description available |
| 16088 | | H | No description available |
| 16089 | | I | No description available |
| 16090 | | J | No description available |
| 16091 | | K | No description available |
| 16092 | | L | No description available |
| 16093 | | M | No description available |
| 16094 | | N | No description available |
| 16095 | | O | No description available |
| 16096 | | P | No description available |
| 16097 | | Q | No description available |
| 16098 | | |
| 16099 | | |
| 16100 | | > open "/Users/becca/Desktop/Postdoc/COPI notebooks and resources/Structure |
| 16101 | | > files/COPI_soluble_job140_dhelix.pdb" |
| 16102 | | |
| 16103 | | Chain information for COPI_soluble_job140_dhelix.pdb #96 |
| 16104 | | --- |
| 16105 | | Chain | Description |
| 16106 | | D | No description available |
| 16107 | | E | No description available |
| 16108 | | F | No description available |
| 16109 | | G | No description available |
| 16110 | | H | No description available |
| 16111 | | I | No description available |
| 16112 | | J | No description available |
| 16113 | | K | No description available |
| 16114 | | L | No description available |
| 16115 | | M | No description available |
| 16116 | | N | No description available |
| 16117 | | O | No description available |
| 16118 | | P | No description available |
| 16119 | | Q | No description available |
| 16120 | | |
| 16121 | | |
| 16122 | | > open "/Users/becca/Desktop/Postdoc/COPI notebooks and resources/Structure |
| 16123 | | > files/COPI_soluble_job150_dhelix.pdb" |
| 16124 | | |
| 16125 | | Chain information for COPI_soluble_job150_dhelix.pdb #97 |
| 16126 | | --- |
| 16127 | | Chain | Description |
| 16128 | | D | No description available |
| 16129 | | E | No description available |
| 16130 | | F | No description available |
| 16131 | | G | No description available |
| 16132 | | H | No description available |
| 16133 | | I | No description available |
| 16134 | | J | No description available |
| 16135 | | K | No description available |
| 16136 | | L | No description available |
| 16137 | | M | No description available |
| 16138 | | N | No description available |
| 16139 | | O | No description available |
| 16140 | | P | No description available |
| 16141 | | Q | No description available |
| 16142 | | |
| 16143 | | |
| 16144 | | > open "/Users/becca/Desktop/Postdoc/COPI notebooks and resources/Structure |
| 16145 | | > files/COPI_soluble_job145_dhelix.pdb" |
| 16146 | | |
| 16147 | | Chain information for COPI_soluble_job145_dhelix.pdb #98 |
| 16148 | | --- |
| 16149 | | Chain | Description |
| 16150 | | D | No description available |
| 16151 | | E | No description available |
| 16152 | | F | No description available |
| 16153 | | G | No description available |
| 16154 | | H | No description available |
| 16155 | | I | No description available |
| 16156 | | J | No description available |
| 16157 | | K | No description available |
| 16158 | | L | No description available |
| 16159 | | M | No description available |
| 16160 | | N | No description available |
| 16161 | | O | No description available |
| 16162 | | P | No description available |
| 16163 | | Q | No description available |
| 16164 | | |
| 16165 | | |
| 16166 | | > ui tool show Matchmaker |
| 16167 | | |
| 16168 | | > matchmaker #89-93#!94-98 to #83 |
| 16169 | | |
| 16170 | | Computing secondary structure |
| 16171 | | [Repeated 4 time(s)] Parameters |
| 16172 | | --- |
| 16173 | | Chain pairing | bb |
| 16174 | | Alignment algorithm | Needleman-Wunsch |
| 16175 | | Similarity matrix | BLOSUM-62 |
| 16176 | | SS fraction | 0.3 |
| 16177 | | Gap open (HH/SS/other) | 18/18/6 |
| 16178 | | Gap extend | 1 |
| 16179 | | SS matrix | | | H | S | O |
| 16180 | | ---|---|---|--- |
| 16181 | | H | 6 | -9 | -6 |
| 16182 | | S | | 6 | -6 |
| 16183 | | O | | | 4 |
| 16184 | | Iteration cutoff | 2 |
| 16185 | | |
| 16186 | | Matchmaker copy of PredictAndBuild_7_overall_best.pdb, chain A (#83) with |
| 16187 | | fold_20250704_copi_abbprimedgz_trucadg_model_0.cif, chain E (#89), sequence |
| 16188 | | alignment score = 2394.6 |
| 16189 | | RMSD between 254 pruned atom pairs is 1.108 angstroms; (across all 431 pairs: |
| 16190 | | 2.715) |
| 16191 | | |
| 16192 | | Matchmaker copy of PredictAndBuild_7_overall_best.pdb, chain A (#83) with |
| 16193 | | fold_20250704_copi_abbprimedgz_trucadg_model_1.cif, chain E (#90), sequence |
| 16194 | | alignment score = 2394.6 |
| 16195 | | RMSD between 252 pruned atom pairs is 1.085 angstroms; (across all 431 pairs: |
| 16196 | | 2.724) |
| 16197 | | |
| 16198 | | Matchmaker copy of PredictAndBuild_7_overall_best.pdb, chain A (#83) with |
| 16199 | | fold_20250704_copi_abbprimedgz_trucadg_model_2.cif, chain E (#91), sequence |
| 16200 | | alignment score = 2391 |
| 16201 | | RMSD between 245 pruned atom pairs is 1.089 angstroms; (across all 431 pairs: |
| 16202 | | 2.930) |
| 16203 | | |
| 16204 | | Matchmaker copy of PredictAndBuild_7_overall_best.pdb, chain A (#83) with |
| 16205 | | fold_20250704_copi_abbprimedgz_trucadg_model_3.cif, chain E (#92), sequence |
| 16206 | | alignment score = 2401.8 |
| 16207 | | RMSD between 243 pruned atom pairs is 1.065 angstroms; (across all 431 pairs: |
| 16208 | | 3.641) |
| 16209 | | |
| 16210 | | Matchmaker copy of PredictAndBuild_7_overall_best.pdb, chain A (#83) with |
| 16211 | | fold_20250704_copi_abbprimedgz_trucadg_model_4.cif, chain E (#93), sequence |
| 16212 | | alignment score = 2401.8 |
| 16213 | | RMSD between 241 pruned atom pairs is 1.095 angstroms; (across all 431 pairs: |
| 16214 | | 2.725) |
| 16215 | | |
| 16216 | | Matchmaker copy of PredictAndBuild_7_overall_best.pdb, chain A (#83) with |
| 16217 | | COPI_soluble_job160_dhelix.pdb, chain M (#94), sequence alignment score = |
| 16218 | | 1452.2 |
| 16219 | | RMSD between 239 pruned atom pairs is 1.088 angstroms; (across all 282 pairs: |
| 16220 | | 1.700) |
| 16221 | | |
| 16222 | | Matchmaker copy of PredictAndBuild_7_overall_best.pdb, chain A (#83) with |
| 16223 | | COPI_soluble_job155_dhelix.pdb, chain M (#95), sequence alignment score = |
| 16224 | | 1452.2 |
| 16225 | | RMSD between 239 pruned atom pairs is 1.088 angstroms; (across all 282 pairs: |
| 16226 | | 1.700) |
| 16227 | | |
| 16228 | | Matchmaker copy of PredictAndBuild_7_overall_best.pdb, chain A (#83) with |
| 16229 | | COPI_soluble_job140_dhelix.pdb, chain M (#96), sequence alignment score = |
| 16230 | | 1452.2 |
| 16231 | | RMSD between 239 pruned atom pairs is 1.088 angstroms; (across all 282 pairs: |
| 16232 | | 1.700) |
| 16233 | | |
| 16234 | | Matchmaker copy of PredictAndBuild_7_overall_best.pdb, chain A (#83) with |
| 16235 | | COPI_soluble_job150_dhelix.pdb, chain M (#97), sequence alignment score = |
| 16236 | | 1452.2 |
| 16237 | | RMSD between 239 pruned atom pairs is 1.088 angstroms; (across all 282 pairs: |
| 16238 | | 1.700) |
| 16239 | | |
| 16240 | | Matchmaker copy of PredictAndBuild_7_overall_best.pdb, chain A (#83) with |
| 16241 | | COPI_soluble_job145_dhelix.pdb, chain M (#98), sequence alignment score = |
| 16242 | | 1452.2 |
| 16243 | | RMSD between 239 pruned atom pairs is 1.088 angstroms; (across all 282 pairs: |
| 16244 | | 1.700) |
| 16245 | | |
| 16246 | | |
| 16247 | | > hide #86-93#!84-85,94-98 atoms |
| 16248 | | |
| 16249 | | > show #86-93#!84-85,94-98 cartoons |
| 16250 | | |
| 16251 | | > hide #93 models |
| 16252 | | |
| 16253 | | > hide #92 models |
| 16254 | | |
| 16255 | | > hide #91 models |
| 16256 | | |
| 16257 | | > hide #90 models |
| 16258 | | |
| 16259 | | > hide #89 models |
| 16260 | | |
| 16261 | | > hide #88 models |
| 16262 | | |
| 16263 | | > hide #87 models |
| 16264 | | |
| 16265 | | > hide #86 models |
| 16266 | | |
| 16267 | | > hide #!85 models |
| 16268 | | |
| 16269 | | > hide #!84 models |
| 16270 | | |
| 16271 | | > hide #!98 models |
| 16272 | | |
| 16273 | | > hide #!97 models |
| 16274 | | |
| 16275 | | > hide #!96 models |
| 16276 | | |
| 16277 | | > hide #!95 models |
| 16278 | | |
| 16279 | | > hide #!94 models |
| 16280 | | |
| 16281 | | > show #89 models |
| 16282 | | |
| 16283 | | > fitmap #89 inMap #25 |
| 16284 | | |
| 16285 | | Fit molecule fold_20250704_copi_abbprimedgz_trucadg_model_0.cif (#89) to map |
| 16286 | | W20_J4027_005_volume_map_gammazeta_local.mrc (#25) using 31307 atoms |
| 16287 | | average map value = 0.1729, steps = 144 |
| 16288 | | shifted from previous position = 3.16 |
| 16289 | | rotated from previous position = 14.3 degrees |
| 16290 | | atoms outside contour = 21652, contour level = 0.2532 |
| 16291 | | |
| 16292 | | Position of fold_20250704_copi_abbprimedgz_trucadg_model_0.cif (#89) relative |
| 16293 | | to W20_J4027_005_volume_map_gammazeta_local.mrc (#25) coordinates: |
| 16294 | | Matrix rotation and translation |
| 16295 | | 0.05328171 -0.76106194 -0.64648727 140.92255080 |
| 16296 | | 0.28101753 0.63267016 -0.72163538 147.85576557 |
| 16297 | | 0.95822242 -0.14322428 0.24758149 143.21536141 |
| 16298 | | Axis 0.28936539 -0.80279829 0.52132770 |
| 16299 | | Axis point 9.80405567 0.00000000 205.08316869 |
| 16300 | | Rotation angle (degrees) 91.90447951 |
| 16301 | | Shift along axis -3.25811224 |
| 16302 | | |
| 16303 | | |
| 16304 | | > hide #89 models |
| 16305 | | |
| 16306 | | > show #!94 models |
| 16307 | | |
| 16308 | | > fitmap #94 inMap #25 |
| 16309 | | |
| 16310 | | Fit molecule COPI_soluble_job160_dhelix.pdb (#94) to map |
| 16311 | | W20_J4027_005_volume_map_gammazeta_local.mrc (#25) using 27657 atoms |
| 16312 | | average map value = 0.2718, steps = 204 |
| 16313 | | shifted from previous position = 15.7 |
| 16314 | | rotated from previous position = 16.6 degrees |
| 16315 | | atoms outside contour = 14003, contour level = 0.2532 |
| 16316 | | |
| 16317 | | Position of COPI_soluble_job160_dhelix.pdb (#94) relative to |
| 16318 | | W20_J4027_005_volume_map_gammazeta_local.mrc (#25) coordinates: |
| 16319 | | Matrix rotation and translation |
| 16320 | | -0.94072620 0.21431989 -0.26287109 429.26780955 |
| 16321 | | 0.04392373 -0.69153884 -0.72100259 611.48757588 |
| 16322 | | -0.33631076 -0.68981230 0.64113497 251.13731297 |
| 16323 | | Axis 0.16577200 0.39032157 -0.90563167 |
| 16324 | | Axis point 252.69703805 349.04589066 0.00000000 |
| 16325 | | Rotation angle (degrees) 174.60186768 |
| 16326 | | Shift along axis 82.39947160 |
| 16327 | | |
| 16328 | | |
| 16329 | | > fitmap #95 inMap #25 |
| 16330 | | |
| 16331 | | Fit molecule COPI_soluble_job155_dhelix.pdb (#95) to map |
| 16332 | | W20_J4027_005_volume_map_gammazeta_local.mrc (#25) using 27657 atoms |
| 16333 | | average map value = 0.2423, steps = 164 |
| 16334 | | shifted from previous position = 15.2 |
| 16335 | | rotated from previous position = 11.8 degrees |
| 16336 | | atoms outside contour = 15830, contour level = 0.2532 |
| 16337 | | |
| 16338 | | Position of COPI_soluble_job155_dhelix.pdb (#95) relative to |
| 16339 | | W20_J4027_005_volume_map_gammazeta_local.mrc (#25) coordinates: |
| 16340 | | Matrix rotation and translation |
| 16341 | | -0.93495608 0.23558445 -0.26524914 421.51639111 |
| 16342 | | 0.02686846 -0.69850918 -0.71509651 616.29442709 |
| 16343 | | -0.35374457 -0.67571066 0.64674561 248.96083246 |
| 16344 | | Axis 0.17116994 0.38459904 -0.90707466 |
| 16345 | | Axis point 252.83551002 347.97043032 0.00000000 |
| 16346 | | Rotation angle (degrees) 173.39355126 |
| 16347 | | Shift along axis 83.35111726 |
| 16348 | | |
| 16349 | | |
| 16350 | | > fitmap #96 inMap #25 |
| 16351 | | |
| 16352 | | Fit molecule COPI_soluble_job140_dhelix.pdb (#96) to map |
| 16353 | | W20_J4027_005_volume_map_gammazeta_local.mrc (#25) using 27657 atoms |
| 16354 | | average map value = 0.2622, steps = 256 |
| 16355 | | shifted from previous position = 18.3 |
| 16356 | | rotated from previous position = 12.3 degrees |
| 16357 | | atoms outside contour = 14721, contour level = 0.2532 |
| 16358 | | |
| 16359 | | Position of COPI_soluble_job140_dhelix.pdb (#96) relative to |
| 16360 | | W20_J4027_005_volume_map_gammazeta_local.mrc (#25) coordinates: |
| 16361 | | Matrix rotation and translation |
| 16362 | | -0.93956714 0.21928330 -0.26292285 427.34338098 |
| 16363 | | 0.03882046 -0.69477206 -0.71818156 612.97301969 |
| 16364 | | -0.34015668 -0.68498658 0.64427232 249.37443237 |
| 16365 | | Axis 0.16673989 0.38794901 -0.90647304 |
| 16366 | | Axis point 252.59063007 348.46957852 0.00000000 |
| 16367 | | Rotation angle (degrees) 174.28724830 |
| 16368 | | Shift along axis 83.00626481 |
| 16369 | | |
| 16370 | | |
| 16371 | | > fitmap #97 inMap #25 |
| 16372 | | |
| 16373 | | Fit molecule COPI_soluble_job150_dhelix.pdb (#97) to map |
| 16374 | | W20_J4027_005_volume_map_gammazeta_local.mrc (#25) using 27657 atoms |
| 16375 | | average map value = 0.2628, steps = 212 |
| 16376 | | shifted from previous position = 21 |
| 16377 | | rotated from previous position = 15.7 degrees |
| 16378 | | atoms outside contour = 14726, contour level = 0.2532 |
| 16379 | | |
| 16380 | | Position of COPI_soluble_job150_dhelix.pdb (#97) relative to |
| 16381 | | W20_J4027_005_volume_map_gammazeta_local.mrc (#25) coordinates: |
| 16382 | | Matrix rotation and translation |
| 16383 | | -0.94025104 0.21735184 -0.26208044 427.88998347 |
| 16384 | | 0.04057084 -0.69272872 -0.72005619 612.48408539 |
| 16385 | | -0.33805619 -0.68766641 0.64252076 250.33040167 |
| 16386 | | Axis 0.16599686 0.38937389 -0.90599836 |
| 16387 | | Axis point 252.53671335 348.85846385 0.00000000 |
| 16388 | | Rotation angle (degrees) 174.40123581 |
| 16389 | | Shift along axis 82.71476836 |
| 16390 | | |
| 16391 | | |
| 16392 | | > fitmap #98 inMap #25 |
| 16393 | | |
| 16394 | | Fit molecule COPI_soluble_job145_dhelix.pdb (#98) to map |
| 16395 | | W20_J4027_005_volume_map_gammazeta_local.mrc (#25) using 27657 atoms |
| 16396 | | average map value = 0.2587, steps = 272 |
| 16397 | | shifted from previous position = 18 |
| 16398 | | rotated from previous position = 11.9 degrees |
| 16399 | | atoms outside contour = 15067, contour level = 0.2532 |
| 16400 | | |
| 16401 | | Position of COPI_soluble_job145_dhelix.pdb (#98) relative to |
| 16402 | | W20_J4027_005_volume_map_gammazeta_local.mrc (#25) coordinates: |
| 16403 | | Matrix rotation and translation |
| 16404 | | -0.93993457 0.21619613 -0.26416328 429.04174114 |
| 16405 | | 0.04215368 -0.69443036 -0.71832412 612.05358105 |
| 16406 | | -0.33874190 -0.68631312 0.64360564 249.64587097 |
| 16407 | | Axis 0.16669352 0.38835946 -0.90630580 |
| 16408 | | Axis point 252.87215354 348.49166546 0.00000000 |
| 16409 | | Rotation angle (degrees) 174.49011360 |
| 16410 | | Shift along axis 82.95977526 |
| 16411 | | |
| 16412 | | |
| 16413 | | > hide #!94 models |
| 16414 | | |
| 16415 | | > show #!94 models |
| 16416 | | |
| 16417 | | > hide #!94 models |
| 16418 | | |
| 16419 | | > show #!95 models |
| 16420 | | |
| 16421 | | > hide #!95 models |
| 16422 | | |
| 16423 | | > show #!94 models |
| 16424 | | |
| 16425 | | > hide #!50 models |
| 16426 | | |
| 16427 | | > show #!33 models |
| 16428 | | |
| 16429 | | > hide #!25 models |
| 16430 | | |
| 16431 | | > hide #!33 models |
| 16432 | | |
| 16433 | | > show #!25 models |
| 16434 | | |
| 16435 | | > close #1 |
| 16436 | | |
| 16437 | | > close #4 |
| 16438 | | |
| 16439 | | > close #6 |
| 16440 | | |
| 16441 | | > close #8 |
| 16442 | | |
| 16443 | | > close #10 |
| 16444 | | |
| 16445 | | > close #12 |
| 16446 | | |
| 16447 | | > close #14 |
| 16448 | | |
| 16449 | | > close #16 |
| 16450 | | |
| 16451 | | > close #18 |
| 16452 | | |
| 16453 | | > close #20 |
| 16454 | | |
| 16455 | | > close #22 |
| 16456 | | |
| 16457 | | > close #24 |
| 16458 | | |
| 16459 | | > close #26 |
| 16460 | | |
| 16461 | | > close #28 |
| 16462 | | |
| 16463 | | > close #41 |
| 16464 | | |
| 16465 | | > hide #!25 models |
| 16466 | | |
| 16467 | | > hide #!94 models |
| 16468 | | |
| 16469 | | > show #!96 models |
| 16470 | | |
| 16471 | | > show #!95 models |
| 16472 | | |
| 16473 | | > hide #!95 models |
| 16474 | | |
| 16475 | | > show #!94 models |
| 16476 | | |
| 16477 | | > hide #!94 models |
| 16478 | | |
| 16479 | | > show #!97 models |
| 16480 | | |
| 16481 | | > hide #!97 models |
| 16482 | | |
| 16483 | | > show #!98 models |
| 16484 | | |
| 16485 | | > hide #!98 models |
| 16486 | | |
| 16487 | | > hide #!96 models |
| 16488 | | |
| 16489 | | > show #!94 models |
| 16490 | | |
| 16491 | | > split #94 chains |
| 16492 | | |
| 16493 | | Split COPI_soluble_job160_dhelix.pdb (#94) into 14 models |
| 16494 | | Chain information for COPI_soluble_job160_dhelix.pdb D #94.1 |
| 16495 | | --- |
| 16496 | | Chain | Description |
| 16497 | | D | No description available |
| 16498 | | |
| 16499 | | Chain information for COPI_soluble_job160_dhelix.pdb E #94.2 |
| 16500 | | --- |
| 16501 | | Chain | Description |
| 16502 | | E | No description available |
| 16503 | | |
| 16504 | | Chain information for COPI_soluble_job160_dhelix.pdb F #94.3 |
| 16505 | | --- |
| 16506 | | Chain | Description |
| 16507 | | F | No description available |
| 16508 | | |
| 16509 | | Chain information for COPI_soluble_job160_dhelix.pdb G #94.4 |
| 16510 | | --- |
| 16511 | | Chain | Description |
| 16512 | | G | No description available |
| 16513 | | |
| 16514 | | Chain information for COPI_soluble_job160_dhelix.pdb H #94.5 |
| 16515 | | --- |
| 16516 | | Chain | Description |
| 16517 | | H | No description available |
| 16518 | | |
| 16519 | | Chain information for COPI_soluble_job160_dhelix.pdb I #94.6 |
| 16520 | | --- |
| 16521 | | Chain | Description |
| 16522 | | I | No description available |
| 16523 | | |
| 16524 | | Chain information for COPI_soluble_job160_dhelix.pdb J #94.7 |
| 16525 | | --- |
| 16526 | | Chain | Description |
| 16527 | | J | No description available |
| 16528 | | |
| 16529 | | Chain information for COPI_soluble_job160_dhelix.pdb K #94.8 |
| 16530 | | --- |
| 16531 | | Chain | Description |
| 16532 | | K | No description available |
| 16533 | | |
| 16534 | | Chain information for COPI_soluble_job160_dhelix.pdb L #94.9 |
| 16535 | | --- |
| 16536 | | Chain | Description |
| 16537 | | L | No description available |
| 16538 | | |
| 16539 | | Chain information for COPI_soluble_job160_dhelix.pdb M #94.10 |
| 16540 | | --- |
| 16541 | | Chain | Description |
| 16542 | | M | No description available |
| 16543 | | |
| 16544 | | Chain information for COPI_soluble_job160_dhelix.pdb N #94.11 |
| 16545 | | --- |
| 16546 | | Chain | Description |
| 16547 | | N | No description available |
| 16548 | | |
| 16549 | | Chain information for COPI_soluble_job160_dhelix.pdb O #94.12 |
| 16550 | | --- |
| 16551 | | Chain | Description |
| 16552 | | O | No description available |
| 16553 | | |
| 16554 | | Chain information for COPI_soluble_job160_dhelix.pdb P #94.13 |
| 16555 | | --- |
| 16556 | | Chain | Description |
| 16557 | | P | No description available |
| 16558 | | |
| 16559 | | Chain information for COPI_soluble_job160_dhelix.pdb Q #94.14 |
| 16560 | | --- |
| 16561 | | Chain | Description |
| 16562 | | Q | No description available |
| 16563 | | |
| 16564 | | |
| 16565 | | > show #!95 models |
| 16566 | | |
| 16567 | | > hide #!95 models |
| 16568 | | |
| 16569 | | > close #95 |
| 16570 | | |
| 16571 | | > close #96 |
| 16572 | | |
| 16573 | | > close #97 |
| 16574 | | |
| 16575 | | > close #98 |
| 16576 | | |
| 16577 | | > hide #94.1 models |
| 16578 | | |
| 16579 | | > hide #94.2 models |
| 16580 | | |
| 16581 | | > hide #94.3 models |
| 16582 | | |
| 16583 | | > hide #94.4 models |
| 16584 | | |
| 16585 | | > hide #94.5 models |
| 16586 | | |
| 16587 | | > hide #!94.6 models |
| 16588 | | |
| 16589 | | > hide #!94.7 models |
| 16590 | | |
| 16591 | | > hide #94.8 models |
| 16592 | | |
| 16593 | | > show #94.8 models |
| 16594 | | |
| 16595 | | > hide #94.8 models |
| 16596 | | |
| 16597 | | > hide #94.12 models |
| 16598 | | |
| 16599 | | > hide #!94.13 models |
| 16600 | | |
| 16601 | | > hide #!94.14 models |
| 16602 | | |
| 16603 | | > show #89 models |
| 16604 | | |
| 16605 | | > show #!25 models |
| 16606 | | |
| 16607 | | > hide #89 models |
| 16608 | | |
| 16609 | | > show #90 models |
| 16610 | | |
| 16611 | | > show #89 models |
| 16612 | | |
| 16613 | | > hide #89 models |
| 16614 | | |
| 16615 | | > hide #90 models |
| 16616 | | |
| 16617 | | > show #91 models |
| 16618 | | |
| 16619 | | > hide #91 models |
| 16620 | | |
| 16621 | | > show #92 models |
| 16622 | | |
| 16623 | | > hide #92 models |
| 16624 | | |
| 16625 | | > show #93 models |
| 16626 | | |
| 16627 | | > hide #93 models |
| 16628 | | |
| 16629 | | > show #88 models |
| 16630 | | |
| 16631 | | > hide #88 models |
| 16632 | | |
| 16633 | | > show #78 models |
| 16634 | | |
| 16635 | | > hide #78 models |
| 16636 | | |
| 16637 | | > show #!81 models |
| 16638 | | |
| 16639 | | > hide #!81 models |
| 16640 | | |
| 16641 | | > show #!81 models |
| 16642 | | |
| 16643 | | > hide #!81 models |
| 16644 | | |
| 16645 | | > show #!82 models |
| 16646 | | |
| 16647 | | > hide #!82 models |
| 16648 | | |
| 16649 | | > show #79 models |
| 16650 | | |
| 16651 | | > hide #79 models |
| 16652 | | |
| 16653 | | > show #80 models |
| 16654 | | |
| 16655 | | > fitmap #80 inMap #25 |
| 16656 | | |
| 16657 | | Fit molecule PredictAndBuild_7_superposed_predicted_untrimmed_cycle_2.pdb |
| 16658 | | (#80) to map W20_J4027_005_volume_map_gammazeta_local.mrc (#25) using 6818 |
| 16659 | | atoms |
| 16660 | | average map value = 0.2002, steps = 28 |
| 16661 | | shifted from previous position = 0.0204 |
| 16662 | | rotated from previous position = 0.023 degrees |
| 16663 | | atoms outside contour = 4363, contour level = 0.2532 |
| 16664 | | |
| 16665 | | Position of PredictAndBuild_7_superposed_predicted_untrimmed_cycle_2.pdb (#80) |
| 16666 | | relative to W20_J4027_005_volume_map_gammazeta_local.mrc (#25) coordinates: |
| 16667 | | Matrix rotation and translation |
| 16668 | | -0.61827994 -0.74501686 -0.25035934 390.17808029 |
| 16669 | | -0.76844646 0.50614879 0.39153982 125.89539509 |
| 16670 | | -0.16498469 0.43446897 -0.88544721 224.70859005 |
| 16671 | | Axis 0.43631396 -0.86771240 -0.23812878 |
| 16672 | | Axis point 223.52898003 0.00000000 100.56670382 |
| 16673 | | Rotation angle (degrees) 177.18018194 |
| 16674 | | Shift along axis 7.48956636 |
| 16675 | | |
| 16676 | | |
| 16677 | | > ui tool show Matchmaker |
| 16678 | | |
| 16679 | | > matchmaker #80 to #94.9 |
| 16680 | | |
| 16681 | | Parameters |
| 16682 | | --- |
| 16683 | | Chain pairing | bb |
| 16684 | | Alignment algorithm | Needleman-Wunsch |
| 16685 | | Similarity matrix | BLOSUM-62 |
| 16686 | | SS fraction | 0.3 |
| 16687 | | Gap open (HH/SS/other) | 18/18/6 |
| 16688 | | Gap extend | 1 |
| 16689 | | SS matrix | | | H | S | O |
| 16690 | | ---|---|---|--- |
| 16691 | | H | 6 | -9 | -6 |
| 16692 | | S | | 6 | -6 |
| 16693 | | O | | | 4 |
| 16694 | | Iteration cutoff | 2 |
| 16695 | | |
| 16696 | | Matchmaker COPI_soluble_job160_dhelix.pdb L, chain L (#94.9) with |
| 16697 | | PredictAndBuild_7_superposed_predicted_untrimmed_cycle_2.pdb, chain A (#80), |
| 16698 | | sequence alignment score = 751 |
| 16699 | | RMSD between 111 pruned atom pairs is 0.761 angstroms; (across all 149 pairs: |
| 16700 | | 2.111) |
| 16701 | | |
| 16702 | | |
| 16703 | | > ui tool show Matchmaker |
| 16704 | | |
| 16705 | | > matchmaker #80 to #94.10 |
| 16706 | | |
| 16707 | | Parameters |
| 16708 | | --- |
| 16709 | | Chain pairing | bb |
| 16710 | | Alignment algorithm | Needleman-Wunsch |
| 16711 | | Similarity matrix | BLOSUM-62 |
| 16712 | | SS fraction | 0.3 |
| 16713 | | Gap open (HH/SS/other) | 18/18/6 |
| 16714 | | Gap extend | 1 |
| 16715 | | SS matrix | | | H | S | O |
| 16716 | | ---|---|---|--- |
| 16717 | | H | 6 | -9 | -6 |
| 16718 | | S | | 6 | -6 |
| 16719 | | O | | | 4 |
| 16720 | | Iteration cutoff | 2 |
| 16721 | | |
| 16722 | | Matchmaker COPI_soluble_job160_dhelix.pdb M, chain M (#94.10) with |
| 16723 | | PredictAndBuild_7_superposed_predicted_untrimmed_cycle_2.pdb, chain A (#80), |
| 16724 | | sequence alignment score = 1461.2 |
| 16725 | | RMSD between 282 pruned atom pairs is 0.718 angstroms; (across all 283 pairs: |
| 16726 | | 0.728) |
| 16727 | | |
| 16728 | | |
| 16729 | | > save "/Users/becca/Desktop/Postdoc/COPI notebooks and |
| 16730 | | > resources/Processing/W20_MM_cyto_COPI/20250706_W20_COPI_cyto_alignments.cxs" |
| 16731 | | |
| 16732 | | > show #89 models |
| 16733 | | |
| 16734 | | > split #89 chains |
| 16735 | | |
| 16736 | | Split fold_20250704_copi_abbprimedgz_trucadg_model_0.cif (#89) into 6 models |
| 16737 | | Chain information for fold_20250704_copi_abbprimedgz_trucadg_model_0.cif A |
| 16738 | | #89.1 |
| 16739 | | --- |
| 16740 | | Chain | Description |
| 16741 | | A | No description available |
| 16742 | | |
| 16743 | | Chain information for fold_20250704_copi_abbprimedgz_trucadg_model_0.cif B |
| 16744 | | #89.2 |
| 16745 | | --- |
| 16746 | | Chain | Description |
| 16747 | | B | No description available |
| 16748 | | |
| 16749 | | Chain information for fold_20250704_copi_abbprimedgz_trucadg_model_0.cif C |
| 16750 | | #89.3 |
| 16751 | | --- |
| 16752 | | Chain | Description |
| 16753 | | C | No description available |
| 16754 | | |
| 16755 | | Chain information for fold_20250704_copi_abbprimedgz_trucadg_model_0.cif D |
| 16756 | | #89.4 |
| 16757 | | --- |
| 16758 | | Chain | Description |
| 16759 | | D | No description available |
| 16760 | | |
| 16761 | | Chain information for fold_20250704_copi_abbprimedgz_trucadg_model_0.cif E |
| 16762 | | #89.5 |
| 16763 | | --- |
| 16764 | | Chain | Description |
| 16765 | | E | No description available |
| 16766 | | |
| 16767 | | Chain information for fold_20250704_copi_abbprimedgz_trucadg_model_0.cif F |
| 16768 | | #89.6 |
| 16769 | | --- |
| 16770 | | Chain | Description |
| 16771 | | F | No description available |
| 16772 | | |
| 16773 | | |
| 16774 | | > show #90 models |
| 16775 | | |
| 16776 | | > hide #90 models |
| 16777 | | |
| 16778 | | > close #90 |
| 16779 | | |
| 16780 | | > close #91-93 |
| 16781 | | |
| 16782 | | > hide #89.1 models |
| 16783 | | |
| 16784 | | > hide #89.2 models |
| 16785 | | |
| 16786 | | > show #89.2 models |
| 16787 | | |
| 16788 | | > hide #89.3 models |
| 16789 | | |
| 16790 | | > hide #89.4 models |
| 16791 | | |
| 16792 | | > hide #89.6 models |
| 16793 | | |
| 16794 | | > hide #80 models |
| 16795 | | |
| 16796 | | > hide #89.2 models |
| 16797 | | |
| 16798 | | > hide #!94 models |
| 16799 | | |
| 16800 | | > show #!94 models |
| 16801 | | |
| 16802 | | > hide #!94 models |
| 16803 | | |
| 16804 | | > show #!94 models |
| 16805 | | |
| 16806 | | > hide #!94 models |
| 16807 | | |
| 16808 | | > show #!94 models |
| 16809 | | |
| 16810 | | > hide #!94 models |
| 16811 | | |
| 16812 | | > combine #89.5 |
| 16813 | | |
| 16814 | | [Repeated 1 time(s)] |
| 16815 | | |
| 16816 | | > hide #!89 models |
| 16817 | | |
| 16818 | | > hide #!25 models |
| 16819 | | |
| 16820 | | > hide #4 models |
| 16821 | | |
| 16822 | | Drag select of 2 residues |
| 16823 | | |
| 16824 | | > select up |
| 16825 | | |
| 16826 | | 41 atoms, 40 bonds, 5 residues, 1 model selected |
| 16827 | | |
| 16828 | | > delete sel |
| 16829 | | |
| 16830 | | Drag select of 23 residues |
| 16831 | | |
| 16832 | | > select up |
| 16833 | | |
| 16834 | | 293 atoms, 299 bonds, 37 residues, 1 model selected |
| 16835 | | |
| 16836 | | > delete sel |
| 16837 | | |
| 16838 | | Drag select of 11 residues, 1 pseudobonds |
| 16839 | | |
| 16840 | | > delete sel |
| 16841 | | |
| 16842 | | > show #!94 models |
| 16843 | | |
| 16844 | | > hide #!94 models |
| 16845 | | |
| 16846 | | > show #!94 models |
| 16847 | | |
| 16848 | | Drag select of 14 residues |
| 16849 | | |
| 16850 | | > select up |
| 16851 | | |
| 16852 | | 308 atoms, 312 bonds, 41 residues, 1 model selected |
| 16853 | | |
| 16854 | | > delete sel |
| 16855 | | |
| 16856 | | > hide #!94 models |
| 16857 | | |
| 16858 | | > show #!94 models |
| 16859 | | |
| 16860 | | > hide #!94 models |
| 16861 | | |
| 16862 | | Drag select of 113 residues |
| 16863 | | |
| 16864 | | > select up |
| 16865 | | |
| 16866 | | 1028 atoms, 1043 bonds, 131 residues, 1 model selected |
| 16867 | | |
| 16868 | | > delete sel |
| 16869 | | |
| 16870 | | > fitmap #1 inMap #25 |
| 16871 | | |
| 16872 | | Fit molecule copy of fold_20250704_copi_abbprimedgz_trucadg_model_0.cif E (#1) |
| 16873 | | to map W20_J4027_005_volume_map_gammazeta_local.mrc (#25) using 3497 atoms |
| 16874 | | average map value = 0.3397, steps = 120 |
| 16875 | | shifted from previous position = 9.43 |
| 16876 | | rotated from previous position = 15.4 degrees |
| 16877 | | atoms outside contour = 1097, contour level = 0.2532 |
| 16878 | | |
| 16879 | | Position of copy of fold_20250704_copi_abbprimedgz_trucadg_model_0.cif E (#1) |
| 16880 | | relative to W20_J4027_005_volume_map_gammazeta_local.mrc (#25) coordinates: |
| 16881 | | Matrix rotation and translation |
| 16882 | | 0.28192072 -0.63906244 -0.71562553 141.48853049 |
| 16883 | | 0.25178912 0.76901734 -0.58754964 138.75926341 |
| 16884 | | 0.92580935 -0.01454430 0.37771089 139.40543708 |
| 16885 | | Axis 0.29331865 -0.84024256 0.45602259 |
| 16886 | | Axis point -12.05331597 0.00000000 214.52876942 |
| 16887 | | Rotation angle (degrees) 77.62410125 |
| 16888 | | Shift along axis -11.51818437 |
| 16889 | | |
| 16890 | | |
| 16891 | | > show #!25 models |
| 16892 | | |
| 16893 | | > hide #!1 models |
| 16894 | | |
| 16895 | | > show #!1 models |
| 16896 | | |
| 16897 | | > hide #!1 models |
| 16898 | | |
| 16899 | | > show #4 models |
| 16900 | | |
| 16901 | | > hide #!25 models |
| 16902 | | |
| 16903 | | > hide #4 models |
| 16904 | | |
| 16905 | | > show #!1 models |
| 16906 | | |
| 16907 | | > show #4 models |
| 16908 | | |
| 16909 | | > show #!25 models |
| 16910 | | |
| 16911 | | > hide #!1 models |
| 16912 | | |
| 16913 | | > hide #!25 models |
| 16914 | | |
| 16915 | | > show #!1 models |
| 16916 | | |
| 16917 | | > hide #!1 models |
| 16918 | | |
| 16919 | | > show #!1 models |
| 16920 | | |
| 16921 | | > hide #!1 models |
| 16922 | | |
| 16923 | | > hide #4 models |
| 16924 | | |
| 16925 | | > show #!1 models |
| 16926 | | |
| 16927 | | > hide #!1 models |
| 16928 | | |
| 16929 | | > show #4 models |
| 16930 | | |
| 16931 | | Drag select of 17 residues |
| 16932 | | |
| 16933 | | > select up |
| 16934 | | |
| 16935 | | 334 atoms, 340 bonds, 42 residues, 1 model selected |
| 16936 | | |
| 16937 | | > delete sel |
| 16938 | | |
| 16939 | | Drag select of 9 residues |
| 16940 | | |
| 16941 | | > select up |
| 16942 | | |
| 16943 | | 183 atoms, 186 bonds, 22 residues, 1 model selected |
| 16944 | | |
| 16945 | | > delete sel |
| 16946 | | |
| 16947 | | Drag select of 19 residues |
| 16948 | | |
| 16949 | | > select up |
| 16950 | | |
| 16951 | | 215 atoms, 218 bonds, 30 residues, 1 model selected |
| 16952 | | |
| 16953 | | > select clear |
| 16954 | | |
| 16955 | | Drag select of 9 residues |
| 16956 | | |
| 16957 | | > delete sel |
| 16958 | | |
| 16959 | | Drag select of 7 residues |
| 16960 | | |
| 16961 | | > select clear |
| 16962 | | |
| 16963 | | Drag select of 13 residues |
| 16964 | | |
| 16965 | | > select clear |
| 16966 | | |
| 16967 | | Drag select of 15 residues |
| 16968 | | |
| 16969 | | > delete sel |
| 16970 | | |
| 16971 | | > show #!25 models |
| 16972 | | |
| 16973 | | > fitmap #4 inMap #25 |
| 16974 | | |
| 16975 | | Fit molecule copy of fold_20250704_copi_abbprimedgz_trucadg_model_0.cif E (#4) |
| 16976 | | to map W20_J4027_005_volume_map_gammazeta_local.mrc (#25) using 4566 atoms |
| 16977 | | average map value = 0.3172, steps = 112 |
| 16978 | | shifted from previous position = 8.14 |
| 16979 | | rotated from previous position = 15.5 degrees |
| 16980 | | atoms outside contour = 1647, contour level = 0.2532 |
| 16981 | | |
| 16982 | | Position of copy of fold_20250704_copi_abbprimedgz_trucadg_model_0.cif E (#4) |
| 16983 | | relative to W20_J4027_005_volume_map_gammazeta_local.mrc (#25) coordinates: |
| 16984 | | Matrix rotation and translation |
| 16985 | | 0.28339787 -0.63805488 -0.71594107 141.54232791 |
| 16986 | | 0.25944422 0.76971940 -0.58328444 139.21027357 |
| 16987 | | 0.92324122 -0.02044520 0.38367649 139.06277017 |
| 16988 | | Axis 0.28838113 -0.83986547 0.45985032 |
| 16989 | | Axis point -12.84705545 0.00000000 215.45471328 |
| 16990 | | Rotation angle (degrees) 77.38510832 |
| 16991 | | Shift along axis -12.15170567 |
| 16992 | | |
| 16993 | | |
| 16994 | | > select #4/E:584 |
| 16995 | | |
| 16996 | | 7 atoms, 6 bonds, 1 residue, 1 model selected |
| 16997 | | |
| 16998 | | > delete sel |
| 16999 | | |
| 17000 | | > select #4/E:583 |
| 17001 | | |
| 17002 | | 7 atoms, 6 bonds, 1 residue, 1 model selected |
| 17003 | | |
| 17004 | | > delete sel |
| 17005 | | |
| 17006 | | > hide #!4 models |
| 17007 | | |
| 17008 | | > show #!4 models |
| 17009 | | |
| 17010 | | > combine #4 |
| 17011 | | |
| 17012 | | > hide #!4 models |
| 17013 | | |
| 17014 | | > rename #4 gamma-COPI_v1 |
| 17015 | | |
| 17016 | | > combine #64 #66 #67 #70 #71 #4 |
| 17017 | | |
| 17018 | | Remapping chain ID 'B' in beta-COPI #66 to 'D' |
| 17019 | | Remapping chain ID 'C' in beta-COPI #66 to 'E' |
| 17020 | | Remapping chain ID 'A' in betaprime-COPI #67 to 'F' |
| 17021 | | Remapping chain ID 'B' in betaprime-COPI #67 to 'G' |
| 17022 | | Remapping chain ID 'C' in betaprime-COPI #67 to 'H' |
| 17023 | | Remapping chain ID 'D' in betaprime-COPI #67 to 'I' |
| 17024 | | Remapping chain ID 'A' in delta-COPI #70 to 'J' |
| 17025 | | Remapping chain ID 'B' in delta-COPI #70 to 'K' |
| 17026 | | Remapping chain ID 'A' in zeta-COPI #71 to 'L' |
| 17027 | | Remapping chain ID 'E' in gamma-COPI_v1 #4 to 'M' |
| 17028 | | |
| 17029 | | > hide #!6 models |
| 17030 | | |
| 17031 | | > fitmap #4 inMap #25 |
| 17032 | | |
| 17033 | | Fit molecule gamma-COPI_v1 (#4) to map |
| 17034 | | W20_J4027_005_volume_map_gammazeta_local.mrc (#25) using 4552 atoms |
| 17035 | | average map value = 0.3176, steps = 40 |
| 17036 | | shifted from previous position = 0.0254 |
| 17037 | | rotated from previous position = 0.0198 degrees |
| 17038 | | atoms outside contour = 1637, contour level = 0.2532 |
| 17039 | | |
| 17040 | | Position of gamma-COPI_v1 (#4) relative to |
| 17041 | | W20_J4027_005_volume_map_gammazeta_local.mrc (#25) coordinates: |
| 17042 | | Matrix rotation and translation |
| 17043 | | 0.28348444 -0.63781373 -0.71612165 141.56919510 |
| 17044 | | 0.25922020 0.76992233 -0.58311620 139.19563791 |
| 17045 | | 0.92327756 -0.02032883 0.38359520 139.07316286 |
| 17046 | | Axis 0.28836144 -0.83999668 0.45962296 |
| 17047 | | Axis point -12.85164212 0.00000000 215.45181715 |
| 17048 | | Rotation angle (degrees) 77.37899637 |
| 17049 | | Shift along axis -12.17955787 |
| 17050 | | |
| 17051 | | |
| 17052 | | > fitmap #4 inMap #25 |
| 17053 | | |
| 17054 | | Fit molecule gamma-COPI_v1 (#4) to map |
| 17055 | | W20_J4027_005_volume_map_gammazeta_local.mrc (#25) using 4552 atoms |
| 17056 | | average map value = 0.3176, steps = 40 |
| 17057 | | shifted from previous position = 0.0216 |
| 17058 | | rotated from previous position = 0.0256 degrees |
| 17059 | | atoms outside contour = 1634, contour level = 0.2532 |
| 17060 | | |
| 17061 | | Position of gamma-COPI_v1 (#4) relative to |
| 17062 | | W20_J4027_005_volume_map_gammazeta_local.mrc (#25) coordinates: |
| 17063 | | Matrix rotation and translation |
| 17064 | | 0.28351557 -0.63806429 -0.71588608 141.55271505 |
| 17065 | | 0.25957296 0.76971101 -0.58323823 139.22244867 |
| 17066 | | 0.92316889 -0.02046756 0.38384929 139.05660502 |
| 17067 | | Axis 0.28835533 -0.83982741 0.45993599 |
| 17068 | | Axis point -12.85662194 0.00000000 215.49138830 |
| 17069 | | Rotation angle (degrees) 77.37682658 |
| 17070 | | Shift along axis -12.14821127 |
| 17071 | | |
| 17072 | | |
| 17073 | | > fitmap #64 inMap #25 |
| 17074 | | |
| 17075 | | Fit molecule alpha-COPI (#64) to map |
| 17076 | | W20_J4027_005_volume_map_gammazeta_local.mrc (#25) using 6628 atoms |
| 17077 | | average map value = 0.3537, steps = 72 |
| 17078 | | shifted from previous position = 0.413 |
| 17079 | | rotated from previous position = 1.38 degrees |
| 17080 | | atoms outside contour = 1857, contour level = 0.2532 |
| 17081 | | |
| 17082 | | Position of alpha-COPI (#64) relative to |
| 17083 | | W20_J4027_005_volume_map_gammazeta_local.mrc (#25) coordinates: |
| 17084 | | Matrix rotation and translation |
| 17085 | | -0.36586447 -0.91732918 -0.15700440 275.35878138 |
| 17086 | | -0.79670780 0.22151549 0.56230559 163.87536925 |
| 17087 | | -0.48104042 0.33081427 -0.81188796 242.97769193 |
| 17088 | | Axis -0.55633921 0.77875030 0.28988732 |
| 17089 | | Axis point 211.74531088 0.00000000 70.70797620 |
| 17090 | | Rotation angle (degrees) 167.99197292 |
| 17091 | | Shift along axis 44.86125869 |
| 17092 | | |
| 17093 | | |
| 17094 | | > fitmap #66 inMap #25 |
| 17095 | | |
| 17096 | | Fit molecule beta-COPI (#66) to map |
| 17097 | | W20_J4027_005_volume_map_gammazeta_local.mrc (#25) using 6621 atoms |
| 17098 | | average map value = 0.2922, steps = 64 |
| 17099 | | shifted from previous position = 0.113 |
| 17100 | | rotated from previous position = 1.01 degrees |
| 17101 | | atoms outside contour = 2335, contour level = 0.2532 |
| 17102 | | |
| 17103 | | Position of beta-COPI (#66) relative to |
| 17104 | | W20_J4027_005_volume_map_gammazeta_local.mrc (#25) coordinates: |
| 17105 | | Matrix rotation and translation |
| 17106 | | -0.33217082 -0.91999498 -0.20801872 277.50726921 |
| 17107 | | -0.77605969 0.14122581 0.61464349 158.87975673 |
| 17108 | | -0.53609132 0.36560157 -0.76088211 242.10904879 |
| 17109 | | Axis -0.57078454 0.75191666 0.32988838 |
| 17110 | | Axis point 215.84062714 0.00000000 64.11235965 |
| 17111 | | Rotation angle (degrees) 167.39915972 |
| 17112 | | Shift along axis 40.93643893 |
| 17113 | | |
| 17114 | | |
| 17115 | | > fitmap #70 inMap #25 |
| 17116 | | |
| 17117 | | Fit molecule delta-COPI (#70) to map |
| 17118 | | W20_J4027_005_volume_map_gammazeta_local.mrc (#25) using 1431 atoms |
| 17119 | | average map value = 0.2517, steps = 140 |
| 17120 | | shifted from previous position = 0.914 |
| 17121 | | rotated from previous position = 5.42 degrees |
| 17122 | | atoms outside contour = 770, contour level = 0.2532 |
| 17123 | | |
| 17124 | | Position of delta-COPI (#70) relative to |
| 17125 | | W20_J4027_005_volume_map_gammazeta_local.mrc (#25) coordinates: |
| 17126 | | Matrix rotation and translation |
| 17127 | | -0.34798043 -0.93749415 -0.00378617 175.64116001 |
| 17128 | | 0.05522224 -0.01646557 -0.99833831 178.10409974 |
| 17129 | | 0.93587399 -0.34761128 0.05750022 154.89993905 |
| 17130 | | Axis 0.42983493 -0.62068846 0.65573452 |
| 17131 | | Axis point -39.53177108 213.69242364 0.00000000 |
| 17132 | | Rotation angle (degrees) 130.80395777 |
| 17133 | | Shift along axis 66.52278419 |
| 17134 | | |
| 17135 | | |
| 17136 | | > fitmap #71 inMap #25 |
| 17137 | | |
| 17138 | | Fit molecule zeta-COPI (#71) to map |
| 17139 | | W20_J4027_005_volume_map_gammazeta_local.mrc (#25) using 1420 atoms |
| 17140 | | average map value = 0.2522, steps = 180 |
| 17141 | | shifted from previous position = 10.5 |
| 17142 | | rotated from previous position = 19.5 degrees |
| 17143 | | atoms outside contour = 661, contour level = 0.2532 |
| 17144 | | |
| 17145 | | Position of zeta-COPI (#71) relative to |
| 17146 | | W20_J4027_005_volume_map_gammazeta_local.mrc (#25) coordinates: |
| 17147 | | Matrix rotation and translation |
| 17148 | | -0.32469740 0.40326773 0.85553887 117.00818860 |
| 17149 | | 0.20978479 -0.85131177 0.48089357 145.18762928 |
| 17150 | | 0.92225916 0.33562393 0.19181929 99.65953140 |
| 17151 | | Axis -0.57881168 -0.26584003 -0.77091253 |
| 17152 | | Axis point 25.03383553 54.96126454 0.00000000 |
| 17153 | | Rotation angle (degrees) 172.79097233 |
| 17154 | | Shift along axis -183.15117020 |
| 17155 | | |
| 17156 | | |
| 17157 | | > show #71 models |
| 17158 | | |
| 17159 | | > hide #71 models |
| 17160 | | |
| 17161 | | > show #71 models |
| 17162 | | |
| 17163 | | > hide #71 models |
| 17164 | | |
| 17165 | | > close #8 |
| 17166 | | |
| 17167 | | > combine #64 #66 #67 #70 #71 #4 |
| 17168 | | |
| 17169 | | Remapping chain ID 'B' in beta-COPI #66 to 'D' |
| 17170 | | Remapping chain ID 'C' in beta-COPI #66 to 'E' |
| 17171 | | Remapping chain ID 'A' in betaprime-COPI #67 to 'F' |
| 17172 | | Remapping chain ID 'B' in betaprime-COPI #67 to 'G' |
| 17173 | | Remapping chain ID 'C' in betaprime-COPI #67 to 'H' |
| 17174 | | Remapping chain ID 'D' in betaprime-COPI #67 to 'I' |
| 17175 | | Remapping chain ID 'A' in delta-COPI #70 to 'J' |
| 17176 | | Remapping chain ID 'B' in delta-COPI #70 to 'K' |
| 17177 | | Remapping chain ID 'A' in zeta-COPI #71 to 'L' |
| 17178 | | Remapping chain ID 'E' in gamma-COPI_v1 #4 to 'M' |
| 17179 | | |
| 17180 | | > rename #8 COPI_cyto_for_J4027_v1 |
| 17181 | | |
| 17182 | | > cd "/Users/becca/Desktop/Postdoc/COPI notebooks and |
| 17183 | | > resources/Processing/W20_MM_cyto_COPI" |
| 17184 | | |
| 17185 | | Current working directory is: /Users/becca/Desktop/Postdoc/COPI notebooks and |
| 17186 | | resources/Processing/W20_MM_cyto_COPI |
| 17187 | | |
| 17188 | | > save COPI_cyto_for_J4027_v1.pdb #8 |
| 17189 | | |
| 17190 | | > open |
| 17191 | | > /Users/becca/Desktop/Postdoc/Phenix/W20_MM_cyto_COPI/CombineFocusedMaps_8/cc_map.ccp4 |
| 17192 | | |
| 17193 | | Opened cc_map.ccp4 as #10, grid size 240,240,240, pixel 1.19, shown at level |
| 17194 | | 0.697, step 1, values float32 |
| 17195 | | |
| 17196 | | > open |
| 17197 | | > /Users/becca/Desktop/Postdoc/Phenix/W20_MM_cyto_COPI/CombineFocusedMaps_8/composite_map.ccp4 |
| 17198 | | |
| 17199 | | Opened composite_map.ccp4 as #12, grid size 240,240,240, pixel 1.19, shown at |
| 17200 | | level 5.35, step 1, values float32 |
| 17201 | | |
| 17202 | | > open |
| 17203 | | > /Users/becca/Desktop/Postdoc/Phenix/W20_MM_cyto_COPI/CombineFocusedMaps_8/composite_map.pdb |
| 17204 | | |
| 17205 | | Summary of feedback from opening |
| 17206 | | /Users/becca/Desktop/Postdoc/Phenix/W20_MM_cyto_COPI/CombineFocusedMaps_8/composite_map.pdb |
| 17207 | | --- |
| 17208 | | warning | Ignored bad PDB record found on line 27366 |
| 17209 | | |
| 17210 | | Chain information for composite_map.pdb #14 |
| 17211 | | --- |
| 17212 | | Chain | Description |
| 17213 | | A | No description available |
| 17214 | | B | No description available |
| 17215 | | C | No description available |
| 17216 | | D | No description available |
| 17217 | | E | No description available |
| 17218 | | F | No description available |
| 17219 | | G | No description available |
| 17220 | | H | No description available |
| 17221 | | I | No description available |
| 17222 | | J | No description available |
| 17223 | | K | No description available |
| 17224 | | L | No description available |
| 17225 | | M | No description available |
| 17226 | | |
| 17227 | | |
| 17228 | | > open |
| 17229 | | > /Users/becca/Desktop/Postdoc/Phenix/W20_MM_cyto_COPI/CombineFocusedMaps_8/contribution_map_1.ccp4 |
| 17230 | | |
| 17231 | | Opened contribution_map_1.ccp4 as #16, grid size 240,240,240, pixel 1.19, |
| 17232 | | shown at level 1, step 1, values float32 |
| 17233 | | |
| 17234 | | > open |
| 17235 | | > /Users/becca/Desktop/Postdoc/Phenix/W20_MM_cyto_COPI/CombineFocusedMaps_8/contribution_map_2.ccp4 |
| 17236 | | |
| 17237 | | Opened contribution_map_2.ccp4 as #18, grid size 240,240,240, pixel 1.19, |
| 17238 | | shown at level 0.47, step 1, values float32 |
| 17239 | | |
| 17240 | | > hide #!8 models |
| 17241 | | |
| 17242 | | > hide #!10 models |
| 17243 | | |
| 17244 | | > hide #!12 models |
| 17245 | | |
| 17246 | | > hide #!14 models |
| 17247 | | |
| 17248 | | > hide #!16 models |
| 17249 | | |
| 17250 | | > hide #!18 models |
| 17251 | | |
| 17252 | | > hide #!25 models |
| 17253 | | |
| 17254 | | > show #!10 models |
| 17255 | | |
| 17256 | | > show #!12 models |
| 17257 | | |
| 17258 | | > show #!14 models |
| 17259 | | |
| 17260 | | > show #!16 models |
| 17261 | | |
| 17262 | | > show #!18 models |
| 17263 | | |
| 17264 | | > close #18 |
| 17265 | | |
| 17266 | | > hide #!14 models |
| 17267 | | |
| 17268 | | > close #10 |
| 17269 | | |
| 17270 | | > close #12 |
| 17271 | | |
| 17272 | | > show #!14 models |
| 17273 | | |
| 17274 | | > hide #!14 models |
| 17275 | | |
| 17276 | | > hide #!16 models |
| 17277 | | |
| 17278 | | > show #!16 models |
| 17279 | | |
| 17280 | | > close #16 |
| 17281 | | |
| 17282 | | > close #14 |
| 17283 | | |
| 17284 | | > show #!4 models |
| 17285 | | |
| 17286 | | > show #!25 models |
| 17287 | | |
| 17288 | | > hide #!25 models |
| 17289 | | |
| 17290 | | > show #!23 models |
| 17291 | | |
| 17292 | | > show #!27 models |
| 17293 | | |
| 17294 | | > show #!25 models |
| 17295 | | |
| 17296 | | > hide #!27 models |
| 17297 | | |
| 17298 | | > show #!32 models |
| 17299 | | |
| 17300 | | > hide #!32 models |
| 17301 | | |
| 17302 | | > show #!33 models |
| 17303 | | |
| 17304 | | > hide #!25 models |
| 17305 | | |
| 17306 | | > hide #!23 models |
| 17307 | | |
| 17308 | | > hide #!33 models |
| 17309 | | |
| 17310 | | > show #!2 models |
| 17311 | | |
| 17312 | | > hide #!2 models |
| 17313 | | |
| 17314 | | > show #!3 models |
| 17315 | | |
| 17316 | | > close #3 |
| 17317 | | |
| 17318 | | > show #!5 models |
| 17319 | | |
| 17320 | | > close #5 |
| 17321 | | |
| 17322 | | > show #!7 models |
| 17323 | | |
| 17324 | | > hide #!7 models |
| 17325 | | |
| 17326 | | > show #!9 models |
| 17327 | | |
| 17328 | | > show #!23 models |
| 17329 | | |
| 17330 | | > show #!25 models |
| 17331 | | |
| 17332 | | > show #!27 models |
| 17333 | | |
| 17334 | | > hide #!9 models |
| 17335 | | |
| 17336 | | > show #!9 models |
| 17337 | | |
| 17338 | | > hide #!9 models |
| 17339 | | |
| 17340 | | > hide #!23 models |
| 17341 | | |
| 17342 | | > hide #!27 models |
| 17343 | | |
| 17344 | | > hide #!25 models |
| 17345 | | |
| 17346 | | > show #!7 models |
| 17347 | | |
| 17348 | | > hide #!7 models |
| 17349 | | |
| 17350 | | > show #!9 models |
| 17351 | | |
| 17352 | | > hide #!9 models |
| 17353 | | |
| 17354 | | > show #!11 models |
| 17355 | | |
| 17356 | | > close #11 |
| 17357 | | |
| 17358 | | > show #!13 models |
| 17359 | | |
| 17360 | | > show #!15 models |
| 17361 | | |
| 17362 | | > hide #!15 models |
| 17363 | | |
| 17364 | | > show #!17 models |
| 17365 | | |
| 17366 | | > hide #!17 models |
| 17367 | | |
| 17368 | | > show #!19 models |
| 17369 | | |
| 17370 | | > show #!17 models |
| 17371 | | |
| 17372 | | > show #!15 models |
| 17373 | | |
| 17374 | | > show #!21 models |
| 17375 | | |
| 17376 | | > hide #!21 models |
| 17377 | | |
| 17378 | | > hide #!19 models |
| 17379 | | |
| 17380 | | > hide #!17 models |
| 17381 | | |
| 17382 | | > hide #!15 models |
| 17383 | | |
| 17384 | | > hide #!13 models |
| 17385 | | |
| 17386 | | > show #!29 models |
| 17387 | | |
| 17388 | | > hide #!29 models |
| 17389 | | |
| 17390 | | > show #!30 models |
| 17391 | | |
| 17392 | | > show #!29 models |
| 17393 | | |
| 17394 | | > show #!31 models |
| 17395 | | |
| 17396 | | > hide #!31 models |
| 17397 | | |
| 17398 | | > hide #!30 models |
| 17399 | | |
| 17400 | | > hide #!29 models |
| 17401 | | |
| 17402 | | > show #!32 models |
| 17403 | | |
| 17404 | | > hide #!4 models |
| 17405 | | |
| 17406 | | > hide #!32 models |
| 17407 | | |
| 17408 | | > show #!13 models |
| 17409 | | |
| 17410 | | > show #!15 models |
| 17411 | | |
| 17412 | | > hide #!15 models |
| 17413 | | |
| 17414 | | > show #!17 models |
| 17415 | | |
| 17416 | | > hide #!17 models |
| 17417 | | |
| 17418 | | > show #!19 models |
| 17419 | | |
| 17420 | | > show #!21 models |
| 17421 | | |
| 17422 | | > hide #!21 models |
| 17423 | | |
| 17424 | | > hide #!19 models |
| 17425 | | |
| 17426 | | > hide #!13 models |
| 17427 | | |
| 17428 | | > hide #!2 models |
| 17429 | | |
| 17430 | | > show #!2 models |
| 17431 | | |
| 17432 | | > hide #!2 models |
| 17433 | | |
| 17434 | | > show #!1 models |
| 17435 | | |
| 17436 | | > hide #!1 models |
| 17437 | | |
| 17438 | | > show #!4 models |
| 17439 | | |
| 17440 | | > show #!7 models |
| 17441 | | |
| 17442 | | > hide #!7 models |
| 17443 | | |
| 17444 | | > close #1 |
| 17445 | | |
| 17446 | | > hide #!4 models |
| 17447 | | |
| 17448 | | > open "/Users/becca/Desktop/Postdoc/COPI notebooks and |
| 17449 | | > resources/Processing/W20_MM_cyto_COPI/cryosparc_P126_J3623_008_volume_map_half_A.mrc" |
| 17450 | | > "/Users/becca/Desktop/Postdoc/COPI notebooks and |
| 17451 | | > resources/Processing/W20_MM_cyto_COPI/cryosparc_P126_J3623_008_volume_map_half_B.mrc" |
| 17452 | | |
| 17453 | | Opened cryosparc_P126_J3623_008_volume_map_half_A.mrc as #1.1, grid size |
| 17454 | | 240,240,240, pixel 1.19, shown at level 0.256, step 1, values float32 |
| 17455 | | Opened cryosparc_P126_J3623_008_volume_map_half_B.mrc as #1.2, grid size |
| 17456 | | 240,240,240, pixel 1.19, shown at level 0.257, step 1, values float32 |
| 17457 | | |
| 17458 | | > hide #!1.1 models |
| 17459 | | |
| 17460 | | > show #!1.1 models |
| 17461 | | |
| 17462 | | > hide #!1.2 models |
| 17463 | | |
| 17464 | | > show #!1.2 models |
| 17465 | | |
| 17466 | | > show #!2 models |
| 17467 | | |
| 17468 | | > hide #!2 models |
| 17469 | | |
| 17470 | | > show #!2 models |
| 17471 | | |
| 17472 | | > volume #1.1 level 0.2608 |
| 17473 | | |
| 17474 | | > volume #1.2 level 0.2582 |
| 17475 | | |
| 17476 | | > hide #!1 models |
| 17477 | | |
| 17478 | | > close #1 |
| 17479 | | |
| 17480 | | > open "/Users/becca/Desktop/Postdoc/COPI notebooks and |
| 17481 | | > resources/Processing/W20_MM_cyto_COPI/J3623_auto_sharpened_map.ccp4" |
| 17482 | | |
| 17483 | | Opened J3623_auto_sharpened_map.ccp4 as #1, grid size 240,240,240, pixel 1.19, |
| 17484 | | shown at level 3.17, step 1, values float32 |
| 17485 | | |
| 17486 | | > hide #!2 models |
| 17487 | | |
| 17488 | | > volume #1 level 2.641 |
| 17489 | | |
| 17490 | | > hide #!1 models |
| 17491 | | |
| 17492 | | > show #!1 models |
| 17493 | | |
| 17494 | | > show #!2 models |
| 17495 | | |
| 17496 | | > hide #!1 models |
| 17497 | | |
| 17498 | | > show #!1 models |
| 17499 | | |
| 17500 | | > open |
| 17501 | | > /Users/becca/Desktop/Postdoc/Phenix/W20_MM_cyto_COPI/ResolveCryoEM_22/denmod_resolution_map.ccp4 |
| 17502 | | |
| 17503 | | Opened denmod_resolution_map.ccp4 as #3, grid size 104,126,128, pixel 1.19, |
| 17504 | | shown at level 11.3, step 1, values float32 |
| 17505 | | |
| 17506 | | > hide #!3 models |
| 17507 | | |
| 17508 | | > show #!3 models |
| 17509 | | |
| 17510 | | > hide #!2 models |
| 17511 | | |
| 17512 | | > hide #!1 models |
| 17513 | | |
| 17514 | | > hide #!3 models |
| 17515 | | |
| 17516 | | > close #3 |
| 17517 | | |
| 17518 | | > open |
| 17519 | | > /Users/becca/Desktop/Postdoc/Phenix/W20_MM_cyto_COPI/ResolveCryoEM_22/J3623_denmod_map.ccp4 |
| 17520 | | |
| 17521 | | Opened J3623_denmod_map.ccp4 as #3, grid size 104,126,128, pixel 1.19, shown |
| 17522 | | at level 0.531, step 1, values float32 |
| 17523 | | |
| 17524 | | > volume #3 level 0.2174 |
| 17525 | | |
| 17526 | | > show #!66 models |
| 17527 | | |
| 17528 | | > show #!64 models |
| 17529 | | |
| 17530 | | > hide #!64 models |
| 17531 | | |
| 17532 | | > show #!64 models |
| 17533 | | |
| 17534 | | > hide #!66 models |
| 17535 | | |
| 17536 | | > hide #!64 models |
| 17537 | | |
| 17538 | | > show #!70 models |
| 17539 | | |
| 17540 | | > volume #3 level 0.1325 |
| 17541 | | |
| 17542 | | > volume #3 level 0.25 |
| 17543 | | |
| 17544 | | > show #!66 models |
| 17545 | | |
| 17546 | | > color #5 gammav3 |
| 17547 | | |
| 17548 | | > color #4 gammav3 |
| 17549 | | |
| 17550 | | > hide #!3 models |
| 17551 | | |
| 17552 | | > close #3 |
| 17553 | | |
| 17554 | | > close #2 |
| 17555 | | |
| 17556 | | > close #1 |
| 17557 | | |
| 17558 | | > open "/Users/becca/Desktop/Postdoc/COPI notebooks and |
| 17559 | | > resources/Processing/W20_MM_cyto_COPI/W20_J3623_consensus_structure.mrc" |
| 17560 | | |
| 17561 | | Opened W20_J3623_consensus_structure.mrc as #1, grid size 240,240,240, pixel |
| 17562 | | 1.19, shown at level 0.257, step 1, values float32 |
| 17563 | | |
| 17564 | | > open "/Users/becca/Desktop/Postdoc/COPI notebooks and |
| 17565 | | > resources/Processing/W20_MM_cyto_COPI/J3623_auto_sharpened_map.ccp4" |
| 17566 | | |
| 17567 | | Opened J3623_auto_sharpened_map.ccp4 as #2, grid size 240,240,240, pixel 1.19, |
| 17568 | | shown at level 3.17, step 1, values float32 |
| 17569 | | |
| 17570 | | > hide #!70 models |
| 17571 | | |
| 17572 | | > hide #!66 models |
| 17573 | | |
| 17574 | | > open "/Users/becca/Desktop/Postdoc/COPI notebooks and |
| 17575 | | > resources/Processing/W20_MM_cyto_COPI/cryosparc_P126_J3623_008_volume_map_half_A.mrc" |
| 17576 | | > "/Users/becca/Desktop/Postdoc/COPI notebooks and |
| 17577 | | > resources/Processing/W20_MM_cyto_COPI/cryosparc_P126_J3623_008_volume_map_half_B.mrc" |
| 17578 | | |
| 17579 | | Opened cryosparc_P126_J3623_008_volume_map_half_A.mrc as #3.1, grid size |
| 17580 | | 240,240,240, pixel 1.19, shown at level 0.256, step 1, values float32 |
| 17581 | | Opened cryosparc_P126_J3623_008_volume_map_half_B.mrc as #3.2, grid size |
| 17582 | | 240,240,240, pixel 1.19, shown at level 0.257, step 1, values float32 |
| 17583 | | |
| 17584 | | > rename #3 J3623_half_maps |
| 17585 | | |
| 17586 | | > combine #4 |
| 17587 | | |
| 17588 | | > close #4 |
| 17589 | | |
| 17590 | | > combine #5 |
| 17591 | | |
| 17592 | | [Repeated 1 time(s)] |
| 17593 | | |
| 17594 | | > close #4 |
| 17595 | | |
| 17596 | | > close #5 |
| 17597 | | |
| 17598 | | > show #!6 models |
| 17599 | | |
| 17600 | | > hide #!6 models |
| 17601 | | |
| 17602 | | > close #6 |
| 17603 | | |
| 17604 | | > show #!7 models |
| 17605 | | |
| 17606 | | > hide #!7 models |
| 17607 | | |
| 17608 | | > close #7 |
| 17609 | | |
| 17610 | | > open "/Users/becca/Desktop/Postdoc/COPI notebooks and |
| 17611 | | > resources/Processing/W20_MM_cyto_COPI/W20_J3987_004_volume_map_csparc_refine_of_relion_job125_bprime_mask.mrc" |
| 17612 | | |
| 17613 | | Opened W20_J3987_004_volume_map_csparc_refine_of_relion_job125_bprime_mask.mrc |
| 17614 | | as #4, grid size 240,240,240, pixel 1.2, shown at level 0.273, step 1, values |
| 17615 | | float32 |
| 17616 | | |
| 17617 | | > select add #4 |
| 17618 | | |
| 17619 | | 2 models selected |
| 17620 | | |
| 17621 | | > ui mousemode right "rotate selected models" |
| 17622 | | |
| 17623 | | > view matrix models |
| 17624 | | > #4,-0.25307,-0.96623,-0.048581,315.98,-0.86086,0.202,0.46702,167.97,-0.44144,0.16001,-0.88291,294.43 |
| 17625 | | |
| 17626 | | > view matrix models |
| 17627 | | > #4,-0.14459,-0.98817,-0.051169,305.09,-0.67606,0.060895,0.73433,127.29,-0.72252,0.14077,-0.67686,306.41 |
| 17628 | | |
| 17629 | | > ui mousemode right "translate selected models" |
| 17630 | | |
| 17631 | | > view matrix models |
| 17632 | | > #4,-0.14459,-0.98817,-0.051169,320.79,-0.67606,0.060895,0.73433,129.24,-0.72252,0.14077,-0.67686,326.78 |
| 17633 | | |
| 17634 | | > fitmap #4 inMap #1 |
| 17635 | | |
| 17636 | | Fit map |
| 17637 | | W20_J3987_004_volume_map_csparc_refine_of_relion_job125_bprime_mask.mrc in map |
| 17638 | | W20_J3623_consensus_structure.mrc using 138206 points |
| 17639 | | correlation = 0.982, correlation about mean = 0.7994, overlap = 2.043e+04 |
| 17640 | | steps = 156, shift = 6.09, angle = 17.9 degrees |
| 17641 | | |
| 17642 | | Position of |
| 17643 | | W20_J3987_004_volume_map_csparc_refine_of_relion_job125_bprime_mask.mrc (#4) |
| 17644 | | relative to W20_J3623_consensus_structure.mrc (#1) coordinates: |
| 17645 | | Matrix rotation and translation |
| 17646 | | -0.33998516 -0.91904572 -0.19941175 353.84995809 |
| 17647 | | -0.77678119 0.15490951 0.61042118 146.12290386 |
| 17648 | | -0.53011420 0.36243344 -0.76656437 277.61520820 |
| 17649 | | Axis -0.56728131 0.75649433 0.32543548 |
| 17650 | | Axis point 252.00716027 0.00000000 81.49850043 |
| 17651 | | Rotation angle (degrees) 167.37461303 |
| 17652 | | Shift along axis 0.15451903 |
| 17653 | | |
| 17654 | | |
| 17655 | | > volume resample #4 onGrid #1 |
| 17656 | | |
| 17657 | | Opened W20_J3987_004_volume_map_csparc_refine_of_relion_job125_bprime_mask.mrc |
| 17658 | | resampled as #5, grid size 240,240,240, pixel 1.19, shown at step 1, values |
| 17659 | | float32 |
| 17660 | | |
| 17661 | | > select subtract #4 |
| 17662 | | |
| 17663 | | Nothing selected |
| 17664 | | |
| 17665 | | > close #4 |
| 17666 | | |
| 17667 | | > cd "/Users/becca/Desktop/Postdoc/COPI notebooks and |
| 17668 | | > resources/Processing/W20_MM_cyto_COPI" |
| 17669 | | |
| 17670 | | Current working directory is: /Users/becca/Desktop/Postdoc/COPI notebooks and |
| 17671 | | resources/Processing/W20_MM_cyto_COPI |
| 17672 | | |
| 17673 | | > save W20_J3987_consensus_resampled.mrc #5 |
| 17674 | | |
| 17675 | | > open "/Users/becca/Desktop/Postdoc/COPI notebooks and |
| 17676 | | > resources/Processing/W20_MM_cyto_COPI/W20_J3987_consensus_resampled.mrc" |
| 17677 | | |
| 17678 | | Opened W20_J3987_consensus_resampled.mrc as #4, grid size 240,240,240, pixel |
| 17679 | | 1.19, shown at level 0.277, step 1, values float32 |
| 17680 | | |
| 17681 | | > hide #!5 models |
| 17682 | | |
| 17683 | | > fitmap #4 inMap #1 |
| 17684 | | |
| 17685 | | Fit map W20_J3987_consensus_resampled.mrc in map |
| 17686 | | W20_J3623_consensus_structure.mrc using 138117 points |
| 17687 | | correlation = 0.9803, correlation about mean = 0.7915, overlap = 2.075e+04 |
| 17688 | | steps = 28, shift = 0.021, angle = 0.00696 degrees |
| 17689 | | |
| 17690 | | Position of W20_J3987_consensus_resampled.mrc (#4) relative to |
| 17691 | | W20_J3623_consensus_structure.mrc (#1) coordinates: |
| 17692 | | Matrix rotation and translation |
| 17693 | | 1.00000000 0.00006999 -0.00003626 -0.00018519 |
| 17694 | | -0.00006999 0.99999999 -0.00009239 0.04429622 |
| 17695 | | 0.00003626 0.00009240 1.00000000 -0.01684920 |
| 17696 | | Axis 0.76076686 -0.29856000 -0.57627746 |
| 17697 | | Axis point 0.00000000 205.18615312 467.59873778 |
| 17698 | | Rotation angle (degrees) 0.00695857 |
| 17699 | | Shift along axis -0.00365615 |
| 17700 | | |
| 17701 | | |
| 17702 | | > close #5 |
| 17703 | | |
| 17704 | | > open "/Users/becca/Desktop/Postdoc/COPI notebooks and |
| 17705 | | > resources/Processing/W20_MM_cyto_COPI/cryosparc_P126_J3987_004_volume_map_half_B.mrc" |
| 17706 | | > "/Users/becca/Desktop/Postdoc/COPI notebooks and |
| 17707 | | > resources/Processing/W20_MM_cyto_COPI/cryosparc_P126_J3987_004_volume_map_half_A.mrc" |
| 17708 | | |
| 17709 | | Opened cryosparc_P126_J3987_004_volume_map_half_B.mrc as #5.1, grid size |
| 17710 | | 240,240,240, pixel 1.2, shown at level 0.274, step 1, values float32 |
| 17711 | | Opened cryosparc_P126_J3987_004_volume_map_half_A.mrc as #5.2, grid size |
| 17712 | | 240,240,240, pixel 1.2, shown at level 0.274, step 1, values float32 |
| 17713 | | |
| 17714 | | > select add #5.1 |
| 17715 | | |
| 17716 | | 2 models selected |
| 17717 | | |
| 17718 | | > select add #5.2 |
| 17719 | | |
| 17720 | | 4 models selected |
| 17721 | | |
| 17722 | | > ui mousemode right "rotate selected models" |
| 17723 | | |
| 17724 | | > view matrix models |
| 17725 | | > #5.1,-0.38208,-0.71776,-0.5821,371.56,-0.90525,0.41738,0.079546,196.1,0.18586,0.55733,-0.80922,143.66,#5.2,-0.38208,-0.71776,-0.5821,371.56,-0.90525,0.41738,0.079546,196.1,0.18586,0.55733,-0.80922,143.66 |
| 17726 | | |
| 17727 | | > view matrix models |
| 17728 | | > #5.1,-0.42487,-0.88152,-0.20596,349.25,-0.72637,0.19619,0.6587,124.33,-0.54025,0.42946,-0.72366,248.21,#5.2,-0.42487,-0.88152,-0.20596,349.25,-0.72637,0.19619,0.6587,124.33,-0.54025,0.42946,-0.72366,248.21 |
| 17729 | | |
| 17730 | | > ui mousemode right "translate selected models" |
| 17731 | | |
| 17732 | | > view matrix models |
| 17733 | | > #5.1,-0.42487,-0.88152,-0.20596,359.35,-0.72637,0.19619,0.6587,126.62,-0.54025,0.42946,-0.72366,262.21,#5.2,-0.42487,-0.88152,-0.20596,359.35,-0.72637,0.19619,0.6587,126.62,-0.54025,0.42946,-0.72366,262.21 |
| 17734 | | |
| 17735 | | > fitmap #5.1 inMap #1 |
| 17736 | | |
| 17737 | | Fit map cryosparc_P126_J3987_004_volume_map_half_B.mrc in map |
| 17738 | | W20_J3623_consensus_structure.mrc using 138219 points |
| 17739 | | correlation = 0.9769, correlation about mean = 0.7541, overlap = 2.056e+04 |
| 17740 | | steps = 80, shift = 1.82, angle = 5.92 degrees |
| 17741 | | |
| 17742 | | Position of cryosparc_P126_J3987_004_volume_map_half_B.mrc (#5.1) relative to |
| 17743 | | W20_J3623_consensus_structure.mrc (#1) coordinates: |
| 17744 | | Matrix rotation and translation |
| 17745 | | -0.34088191 -0.91883476 -0.19885219 353.85897435 |
| 17746 | | -0.77626012 0.15578649 0.61086069 145.85323816 |
| 17747 | | -0.53030155 0.36259239 -0.76635958 277.59067344 |
| 17748 | | Axis -0.56685311 0.75677442 0.32553039 |
| 17749 | | Axis point 251.87895379 0.00000000 81.50551240 |
| 17750 | | Rotation angle (degrees) 167.35038692 |
| 17751 | | Shift along axis 0.15614218 |
| 17752 | | |
| 17753 | | |
| 17754 | | > fitmap #5.2 inMap #1 |
| 17755 | | |
| 17756 | | Fit map cryosparc_P126_J3987_004_volume_map_half_A.mrc in map |
| 17757 | | W20_J3623_consensus_structure.mrc using 138231 points |
| 17758 | | correlation = 0.9769, correlation about mean = 0.7539, overlap = 2.061e+04 |
| 17759 | | steps = 80, shift = 1.81, angle = 6.04 degrees |
| 17760 | | |
| 17761 | | Position of cryosparc_P126_J3987_004_volume_map_half_A.mrc (#5.2) relative to |
| 17762 | | W20_J3623_consensus_structure.mrc (#1) coordinates: |
| 17763 | | Matrix rotation and translation |
| 17764 | | -0.33893365 -0.91937774 -0.19967107 353.79911788 |
| 17765 | | -0.77742964 0.15417077 0.60978235 146.41544867 |
| 17766 | | -0.52983688 0.36190597 -0.76700517 277.70182392 |
| 17767 | | Axis -0.56776952 0.75625634 0.32513709 |
| 17768 | | Axis point 252.14174450 0.00000000 81.52863602 |
| 17769 | | Rotation angle (degrees) 167.39140634 |
| 17770 | | Shift along axis 0.14242009 |
| 17771 | | |
| 17772 | | |
| 17773 | | > select subtract #5.2 |
| 17774 | | |
| 17775 | | 2 models selected |
| 17776 | | |
| 17777 | | > select subtract #5.1 |
| 17778 | | |
| 17779 | | Nothing selected |
| 17780 | | |
| 17781 | | > volume resample #5.1 #5.2 onGrid #1 |
| 17782 | | |
| 17783 | | Opened cryosparc_P126_J3987_004_volume_map_half_B.mrc resampled as #6, grid |
| 17784 | | size 240,240,240, pixel 1.19, shown at step 1, values float32 |
| 17785 | | Opened cryosparc_P126_J3987_004_volume_map_half_A.mrc resampled as #7, grid |
| 17786 | | size 240,240,240, pixel 1.19, shown at step 1, values float32 |
| 17787 | | |
| 17788 | | > save W20_J3987_halfA_consensus_resampled.mrc #7 |
| 17789 | | |
| 17790 | | > save W20_J3987_halfB_consensus_resampled.mrc #6 |
| 17791 | | |
| 17792 | | > close #5-7#5.1-2 |
| 17793 | | |
| 17794 | | > open "/Users/becca/Desktop/Postdoc/COPI notebooks and |
| 17795 | | > resources/Processing/W20_MM_cyto_COPI/W20_J3987_halfA_consensus_resampled.mrc" |
| 17796 | | > "/Users/becca/Desktop/Postdoc/COPI notebooks and |
| 17797 | | > resources/Processing/W20_MM_cyto_COPI/W20_J3987_halfB_consensus_resampled.mrc" |
| 17798 | | |
| 17799 | | Opened W20_J3987_halfA_consensus_resampled.mrc as #5.1, grid size 240,240,240, |
| 17800 | | pixel 1.19, shown at level 0.276, step 1, values float32 |
| 17801 | | Opened W20_J3987_halfB_consensus_resampled.mrc as #5.2, grid size 240,240,240, |
| 17802 | | pixel 1.19, shown at level 0.276, step 1, values float32 |
| 17803 | | |
| 17804 | | > rename #5 J3987_half_maps |
| 17805 | | |
| 17806 | | > show #!8 models |
| 17807 | | |
| 17808 | | > hide #!8 models |
| 17809 | | |
| 17810 | | > combine #8 |
| 17811 | | |
| 17812 | | [Repeated 2 time(s)] |
| 17813 | | |
| 17814 | | > close #6-8 |
| 17815 | | |
| 17816 | | > open "/Users/becca/Desktop/Postdoc/COPI notebooks and |
| 17817 | | > resources/Processing/W20_MM_cyto_COPI/W20_J4021_005_volume_map_bprime_local.mrc" |
| 17818 | | |
| 17819 | | Opened W20_J4021_005_volume_map_bprime_local.mrc as #6, grid size 240,240,240, |
| 17820 | | pixel 1.19, shown at level 0.26, step 1, values float32 |
| 17821 | | |
| 17822 | | > open "/Users/becca/Desktop/Postdoc/COPI notebooks and |
| 17823 | | > resources/Processing/W20_MM_cyto_COPI/cryosparc_P126_J4021_005_volume_map_half_B.mrc" |
| 17824 | | |
| 17825 | | Opened cryosparc_P126_J4021_005_volume_map_half_B.mrc as #7, grid size |
| 17826 | | 240,240,240, pixel 1.19, shown at level 0.263, step 1, values float32 |
| 17827 | | |
| 17828 | | > open "/Users/becca/Desktop/Postdoc/COPI notebooks and |
| 17829 | | > resources/Processing/W20_MM_cyto_COPI/cryosparc_P126_J4021_005_volume_map_half_A.mrc" |
| 17830 | | |
| 17831 | | Opened cryosparc_P126_J4021_005_volume_map_half_A.mrc as #8, grid size |
| 17832 | | 240,240,240, pixel 1.19, shown at level 0.262, step 1, values float32 |
| 17833 | | |
| 17834 | | > volume resample #6 #7 #8 onGrid #1 |
| 17835 | | |
| 17836 | | Opened W20_J4021_005_volume_map_bprime_local.mrc resampled as #12, grid size |
| 17837 | | 240,240,240, pixel 1.19, shown at step 1, values float32 |
| 17838 | | Opened cryosparc_P126_J4021_005_volume_map_half_B.mrc resampled as #14, grid |
| 17839 | | size 240,240,240, pixel 1.19, shown at step 1, values float32 |
| 17840 | | Opened cryosparc_P126_J4021_005_volume_map_half_A.mrc resampled as #16, grid |
| 17841 | | size 240,240,240, pixel 1.19, shown at step 1, values float32 |
| 17842 | | |
| 17843 | | > hide #!16 models |
| 17844 | | |
| 17845 | | > show #!16 models |
| 17846 | | |
| 17847 | | > hide #!16 models |
| 17848 | | |
| 17849 | | > show #!16 models |
| 17850 | | |
| 17851 | | > hide #!16 models |
| 17852 | | |
| 17853 | | > show #!16 models |
| 17854 | | |
| 17855 | | > close #16 |
| 17856 | | |
| 17857 | | > close #14 |
| 17858 | | |
| 17859 | | > close #12 |
| 17860 | | |
| 17861 | | > hide #!5 models |
| 17862 | | |
| 17863 | | > hide #!4 models |
| 17864 | | |
| 17865 | | > hide #!3 models |
| 17866 | | |
| 17867 | | > hide #!2 models |
| 17868 | | |
| 17869 | | > fitmap #6 #7 #8 inMap #1 |
| 17870 | | |
| 17871 | | Multiple maps for #6#7#8 |
| 17872 | | |
| 17873 | | > show #!6 models |
| 17874 | | |
| 17875 | | > show #!7 models |
| 17876 | | |
| 17877 | | > show #!8 models |
| 17878 | | |
| 17879 | | > fitmap #6 inMap #1 |
| 17880 | | |
| 17881 | | Fit map W20_J4021_005_volume_map_bprime_local.mrc in map |
| 17882 | | W20_J3623_consensus_structure.mrc using 138166 points |
| 17883 | | correlation = 0.9132, correlation about mean = 0.2137, overlap = 1.684e+04 |
| 17884 | | steps = 52, shift = 0.553, angle = 0.278 degrees |
| 17885 | | |
| 17886 | | Position of W20_J4021_005_volume_map_bprime_local.mrc (#6) relative to |
| 17887 | | W20_J3623_consensus_structure.mrc (#1) coordinates: |
| 17888 | | Matrix rotation and translation |
| 17889 | | 0.99999230 0.00368913 -0.00133441 -0.32621193 |
| 17890 | | -0.00369294 0.99998910 -0.00285852 0.49731748 |
| 17891 | | 0.00132385 0.00286342 0.99999502 -0.37762592 |
| 17892 | | Axis 0.58922795 -0.27373864 -0.76018260 |
| 17893 | | Axis point 131.36464990 82.10529237 0.00000000 |
| 17894 | | Rotation angle (degrees) 0.27819836 |
| 17895 | | Shift along axis -0.04128355 |
| 17896 | | |
| 17897 | | |
| 17898 | | > fitmap #7 inMap #1 |
| 17899 | | |
| 17900 | | Fit map cryosparc_P126_J4021_005_volume_map_half_B.mrc in map |
| 17901 | | W20_J3623_consensus_structure.mrc using 138066 points |
| 17902 | | correlation = 0.907, correlation about mean = 0.2359, overlap = 1.707e+04 |
| 17903 | | steps = 68, shift = 0.546, angle = 0.314 degrees |
| 17904 | | |
| 17905 | | Position of cryosparc_P126_J4021_005_volume_map_half_B.mrc (#7) relative to |
| 17906 | | W20_J3623_consensus_structure.mrc (#1) coordinates: |
| 17907 | | Matrix rotation and translation |
| 17908 | | 0.99999065 0.00386110 -0.00194507 -0.25775142 |
| 17909 | | -0.00386765 0.99998683 -0.00337455 0.61622992 |
| 17910 | | 0.00193201 0.00338205 0.99999241 -0.53165616 |
| 17911 | | Axis 0.61572178 -0.35331456 -0.70431208 |
| 17912 | | Axis point 158.92151900 66.82584882 0.00000000 |
| 17913 | | Rotation angle (degrees) 0.31436809 |
| 17914 | | Shift along axis -0.00197431 |
| 17915 | | |
| 17916 | | |
| 17917 | | > fitmap #8 inMap #1 |
| 17918 | | |
| 17919 | | Fit map cryosparc_P126_J4021_005_volume_map_half_A.mrc in map |
| 17920 | | W20_J3623_consensus_structure.mrc using 138185 points |
| 17921 | | correlation = 0.9063, correlation about mean = 0.2362, overlap = 1.703e+04 |
| 17922 | | steps = 44, shift = 0.534, angle = 0.288 degrees |
| 17923 | | |
| 17924 | | Position of cryosparc_P126_J4021_005_volume_map_half_A.mrc (#8) relative to |
| 17925 | | W20_J3623_consensus_structure.mrc (#1) coordinates: |
| 17926 | | Matrix rotation and translation |
| 17927 | | 0.99999293 0.00344362 -0.00151137 -0.26561114 |
| 17928 | | -0.00344864 0.99998850 -0.00333361 0.55833592 |
| 17929 | | 0.00149988 0.00333880 0.99999330 -0.46926499 |
| 17930 | | Axis 0.66362765 -0.29949438 -0.68549359 |
| 17931 | | Axis point 159.76195443 73.25690596 0.00000000 |
| 17932 | | Rotation angle (degrees) 0.28804014 |
| 17933 | | Shift along axis -0.02180722 |
| 17934 | | |
| 17935 | | |
| 17936 | | > volume resample #6 #7 #8 onGrid #1 |
| 17937 | | |
| 17938 | | Opened W20_J4021_005_volume_map_bprime_local.mrc resampled as #12, grid size |
| 17939 | | 240,240,240, pixel 1.19, shown at step 1, values float32 |
| 17940 | | Opened cryosparc_P126_J4021_005_volume_map_half_B.mrc resampled as #14, grid |
| 17941 | | size 240,240,240, pixel 1.19, shown at step 1, values float32 |
| 17942 | | Opened cryosparc_P126_J4021_005_volume_map_half_A.mrc resampled as #16, grid |
| 17943 | | size 240,240,240, pixel 1.19, shown at step 1, values float32 |
| 17944 | | |
| 17945 | | > hide #!10 models |
| 17946 | | |
| 17947 | | > hide #!11 models |
| 17948 | | |
| 17949 | | > close #13 |
| 17950 | | |
| 17951 | | > save W20_J4021_halfA_consensus_resampled.mrc #16 |
| 17952 | | |
| 17953 | | > save W20_J4021_halfB_consensus_resampled.mrc #14 |
| 17954 | | |
| 17955 | | > save W20_J4021_consensus_resampled.mrc #12 |
| 17956 | | |
| 17957 | | > close #6-8 |
| 17958 | | |
| 17959 | | > open "/Users/becca/Desktop/Postdoc/COPI notebooks and |
| 17960 | | > resources/Processing/W20_MM_cyto_COPI/W20_J4021_consensus_resampled.mrc" |
| 17961 | | |
| 17962 | | Opened W20_J4021_consensus_resampled.mrc as #6, grid size 240,240,240, pixel |
| 17963 | | 1.19, shown at level 0.259, step 1, values float32 |
| 17964 | | |
| 17965 | | > open "/Users/becca/Desktop/Postdoc/COPI notebooks and |
| 17966 | | > resources/Processing/W20_MM_cyto_COPI/W20_J4021_halfA_consensus_resampled.mrc" |
| 17967 | | > "/Users/becca/Desktop/Postdoc/COPI notebooks and |
| 17968 | | > resources/Processing/W20_MM_cyto_COPI/W20_J4021_halfB_consensus_resampled.mrc" |
| 17969 | | |
| 17970 | | Opened W20_J4021_halfA_consensus_resampled.mrc as #7.1, grid size 240,240,240, |
| 17971 | | pixel 1.19, shown at level 0.26, step 1, values float32 |
| 17972 | | Opened W20_J4021_halfB_consensus_resampled.mrc as #7.2, grid size 240,240,240, |
| 17973 | | pixel 1.19, shown at level 0.261, step 1, values float32 |
| 17974 | | |
| 17975 | | > rename #7 J4021_half_maps |
| 17976 | | |
| 17977 | | > combine #10 |
| 17978 | | |
| 17979 | | [Repeated 1 time(s)] |
| 17980 | | |
| 17981 | | > combine #11 |
| 17982 | | |
| 17983 | | > close #8 |
| 17984 | | |
| 17985 | | > close #9 |
| 17986 | | |
| 17987 | | > hide #!18 models |
| 17988 | | |
| 17989 | | > hide #!16 models |
| 17990 | | |
| 17991 | | > hide #!14 models |
| 17992 | | |
| 17993 | | > hide #!13 models |
| 17994 | | |
| 17995 | | > hide #!12 models |
| 17996 | | |
| 17997 | | > hide #!7 models |
| 17998 | | |
| 17999 | | > hide #!6 models |
| 18000 | | |
| 18001 | | > open "/Users/becca/Desktop/Postdoc/COPI notebooks and |
| 18002 | | > resources/Processing/W20_MM_cyto_COPI/W20_J4022_005_volume_map_bprime_local.mrc" |
| 18003 | | |
| 18004 | | Opened W20_J4022_005_volume_map_bprime_local.mrc as #8, grid size 240,240,240, |
| 18005 | | pixel 1.19, shown at level 0.256, step 1, values float32 |
| 18006 | | |
| 18007 | | > open "/Users/becca/Desktop/Postdoc/COPI notebooks and |
| 18008 | | > resources/Processing/W20_MM_cyto_COPI/cryosparc_P126_J4022_005_volume_map_half_A.mrc" |
| 18009 | | |
| 18010 | | Opened cryosparc_P126_J4022_005_volume_map_half_A.mrc as #9, grid size |
| 18011 | | 240,240,240, pixel 1.19, shown at level 0.259, step 1, values float32 |
| 18012 | | |
| 18013 | | > open "/Users/becca/Desktop/Postdoc/COPI notebooks and |
| 18014 | | > resources/Processing/W20_MM_cyto_COPI/cryosparc_P126_J4022_005_volume_map_half_B.mrc" |
| 18015 | | |
| 18016 | | Opened cryosparc_P126_J4022_005_volume_map_half_B.mrc as #20, grid size |
| 18017 | | 240,240,240, pixel 1.19, shown at level 0.257, step 1, values float32 |
| 18018 | | |
| 18019 | | > open "/Users/becca/Desktop/Postdoc/COPI notebooks and |
| 18020 | | > resources/Processing/W20_MM_cyto_COPI/cryosparc_P126_J4022_005_volume_map_half_B.mrc" |
| 18021 | | |
| 18022 | | Opened cryosparc_P126_J4022_005_volume_map_half_B.mrc as #22, grid size |
| 18023 | | 240,240,240, pixel 1.19, shown at level 0.257, step 1, values float32 |
| 18024 | | |
| 18025 | | > close #22 |
| 18026 | | |
| 18027 | | > close #20 |
| 18028 | | |
| 18029 | | > close #10 |
| 18030 | | |
| 18031 | | > close #11 |
| 18032 | | |
| 18033 | | > open "/Users/becca/Desktop/Postdoc/COPI notebooks and |
| 18034 | | > resources/Processing/W20_MM_cyto_COPI/cryosparc_P126_J4022_005_volume_map_half_B.mrc" |
| 18035 | | |
| 18036 | | Opened cryosparc_P126_J4022_005_volume_map_half_B.mrc as #10, grid size |
| 18037 | | 240,240,240, pixel 1.19, shown at level 0.257, step 1, values float32 |
| 18038 | | |
| 18039 | | > fitmap #8 inMap #1 |
| 18040 | | |
| 18041 | | Fit map W20_J4022_005_volume_map_bprime_local.mrc in map |
| 18042 | | W20_J3623_consensus_structure.mrc using 138200 points |
| 18043 | | correlation = 0.9195, correlation about mean = 0.2263, overlap = 1.667e+04 |
| 18044 | | steps = 64, shift = 0.329, angle = 0.376 degrees |
| 18045 | | |
| 18046 | | Position of W20_J4022_005_volume_map_bprime_local.mrc (#8) relative to |
| 18047 | | W20_J3623_consensus_structure.mrc (#1) coordinates: |
| 18048 | | Matrix rotation and translation |
| 18049 | | 0.99997966 0.00237299 0.00592048 -1.40324868 |
| 18050 | | -0.00238223 0.99999595 0.00155423 -0.17561136 |
| 18051 | | -0.00591677 -0.00156830 0.99998127 1.18884605 |
| 18052 | | Axis -0.23775685 0.90131776 -0.36207456 |
| 18053 | | Axis point 184.53246236 0.00000000 247.89429522 |
| 18054 | | Rotation angle (degrees) 0.37624341 |
| 18055 | | Shift along axis -0.25510056 |
| 18056 | | |
| 18057 | | |
| 18058 | | > fitmap #9 inMap #1 |
| 18059 | | |
| 18060 | | Fit map cryosparc_P126_J4022_005_volume_map_half_A.mrc in map |
| 18061 | | W20_J3623_consensus_structure.mrc using 138165 points |
| 18062 | | correlation = 0.9121, correlation about mean = 0.2433, overlap = 1.687e+04 |
| 18063 | | steps = 52, shift = 0.339, angle = 0.357 degrees |
| 18064 | | |
| 18065 | | Position of cryosparc_P126_J4022_005_volume_map_half_A.mrc (#9) relative to |
| 18066 | | W20_J3623_consensus_structure.mrc (#1) coordinates: |
| 18067 | | Matrix rotation and translation |
| 18068 | | 0.99998304 0.00160770 0.00559706 -1.23594018 |
| 18069 | | -0.00161999 0.99999628 0.00219278 -0.39387001 |
| 18070 | | -0.00559351 -0.00220181 0.99998193 1.22871953 |
| 18071 | | Axis -0.35302911 0.89896616 -0.25928803 |
| 18072 | | Axis point 207.94947596 0.00000000 236.35689026 |
| 18073 | | Rotation angle (degrees) 0.35661877 |
| 18074 | | Shift along axis -0.23634521 |
| 18075 | | |
| 18076 | | |
| 18077 | | > fitmap #10 inMap #1 |
| 18078 | | |
| 18079 | | Fit map cryosparc_P126_J4022_005_volume_map_half_B.mrc in map |
| 18080 | | W20_J3623_consensus_structure.mrc using 138076 points |
| 18081 | | correlation = 0.9128, correlation about mean = 0.2508, overlap = 1.688e+04 |
| 18082 | | steps = 64, shift = 0.335, angle = 0.335 degrees |
| 18083 | | |
| 18084 | | Position of cryosparc_P126_J4022_005_volume_map_half_B.mrc (#10) relative to |
| 18085 | | W20_J3623_consensus_structure.mrc (#1) coordinates: |
| 18086 | | Matrix rotation and translation |
| 18087 | | 0.99998354 0.00229208 0.00525924 -1.29178101 |
| 18088 | | -0.00229810 0.99999671 0.00113989 -0.13676617 |
| 18089 | | -0.00525661 -0.00115196 0.99998552 0.99455043 |
| 18090 | | Axis -0.19587427 0.89873969 -0.39230121 |
| 18091 | | Axis point 169.08755660 -0.00000000 255.83594172 |
| 18092 | | Rotation angle (degrees) 0.33520093 |
| 18093 | | Shift along axis -0.26005386 |
| 18094 | | |
| 18095 | | |
| 18096 | | > volume resample #8 #9 #10 onGrid #1 |
| 18097 | | |
| 18098 | | Opened W20_J4022_005_volume_map_bprime_local.mrc resampled as #11, grid size |
| 18099 | | 240,240,240, pixel 1.19, shown at step 1, values float32 |
| 18100 | | Opened cryosparc_P126_J4022_005_volume_map_half_A.mrc resampled as #20, grid |
| 18101 | | size 240,240,240, pixel 1.19, shown at step 1, values float32 |
| 18102 | | Opened cryosparc_P126_J4022_005_volume_map_half_B.mrc resampled as #22, grid |
| 18103 | | size 240,240,240, pixel 1.19, shown at step 1, values float32 |
| 18104 | | |
| 18105 | | > save W20_J4022_halfB_consensus_resampled.mrc #22 |
| 18106 | | |
| 18107 | | > save W20_J4022_half1_consensus_resampled.mrc #20 |
| 18108 | | |
| 18109 | | > save W20_J4022_halfA_consensus_resampled.mrc #20 |
| 18110 | | |
| 18111 | | > save W20_J4022_consensus_resampled.mrc #11 |
| 18112 | | |
| 18113 | | > close #8-10 |
| 18114 | | |
| 18115 | | > open "/Users/becca/Desktop/Postdoc/COPI notebooks and |
| 18116 | | > resources/Processing/W20_MM_cyto_COPI/W20_J4022_consensus_resampled.mrc" |
| 18117 | | |
| 18118 | | Opened W20_J4022_consensus_resampled.mrc as #8, grid size 240,240,240, pixel |
| 18119 | | 1.19, shown at level 0.256, step 1, values float32 |
| 18120 | | |
| 18121 | | > open "/Users/becca/Desktop/Postdoc/COPI notebooks and |
| 18122 | | > resources/Processing/W20_MM_cyto_COPI/W20_J4022_halfA_consensus_resampled.mrc" |
| 18123 | | > "/Users/becca/Desktop/Postdoc/COPI notebooks and |
| 18124 | | > resources/Processing/W20_MM_cyto_COPI/W20_J4022_halfB_consensus_resampled.mrc" |
| 18125 | | |
| 18126 | | Opened W20_J4022_halfA_consensus_resampled.mrc as #9.1, grid size 240,240,240, |
| 18127 | | pixel 1.19, shown at level 0.257, step 1, values float32 |
| 18128 | | Opened W20_J4022_halfB_consensus_resampled.mrc as #9.2, grid size 240,240,240, |
| 18129 | | pixel 1.19, shown at level 0.255, step 1, values float32 |
| 18130 | | |
| 18131 | | > rename #9 J4022_half_maps |
| 18132 | | |
| 18133 | | > combine #13 |
| 18134 | | |
| 18135 | | [Repeated 1 time(s)] |
| 18136 | | |
| 18137 | | > combine #18 |
| 18138 | | |
| 18139 | | > hide #!10 models |
| 18140 | | |
| 18141 | | > close #10-17 |
| 18142 | | |
| 18143 | | > hide #!9 models |
| 18144 | | |
| 18145 | | > hide #!8 models |
| 18146 | | |
| 18147 | | > show #!25 models |
| 18148 | | |
| 18149 | | > hide #!24 models |
| 18150 | | |
| 18151 | | > hide #!26 models |
| 18152 | | |
| 18153 | | > hide #!25 models |
| 18154 | | |
| 18155 | | > hide #!22 models |
| 18156 | | |
| 18157 | | > hide #!20 models |
| 18158 | | |
| 18159 | | > open "/Users/becca/Desktop/Postdoc/COPI notebooks and |
| 18160 | | > resources/Processing/W20_MM_cyto_COPI/W20_J4023_003_volume_map_bprime_local.mrc" |
| 18161 | | |
| 18162 | | Opened W20_J4023_003_volume_map_bprime_local.mrc as #10, grid size |
| 18163 | | 240,240,240, pixel 1.19, shown at level 0.26, step 1, values float32 |
| 18164 | | |
| 18165 | | > open "/Users/becca/Desktop/Postdoc/COPI notebooks and |
| 18166 | | > resources/Processing/W20_MM_cyto_COPI/cryosparc_P126_J4023_003_volume_map_half_A.mrc" |
| 18167 | | |
| 18168 | | Opened cryosparc_P126_J4023_003_volume_map_half_A.mrc as #11, grid size |
| 18169 | | 240,240,240, pixel 1.19, shown at level 0.26, step 1, values float32 |
| 18170 | | |
| 18171 | | > open "/Users/becca/Desktop/Postdoc/COPI notebooks and |
| 18172 | | > resources/Processing/W20_MM_cyto_COPI/cryosparc_P126_J4023_003_volume_map_half_B.mrc" |
| 18173 | | |
| 18174 | | Opened cryosparc_P126_J4023_003_volume_map_half_B.mrc as #12, grid size |
| 18175 | | 240,240,240, pixel 1.19, shown at level 0.261, step 1, values float32 |
| 18176 | | |
| 18177 | | > fitmap #10 inMap #1 |
| 18178 | | |
| 18179 | | Fit map W20_J4023_003_volume_map_bprime_local.mrc in map |
| 18180 | | W20_J3623_consensus_structure.mrc using 138098 points |
| 18181 | | correlation = 0.9217, correlation about mean = 0.2228, overlap = 1.65e+04 |
| 18182 | | steps = 52, shift = 0.377, angle = 0.32 degrees |
| 18183 | | |
| 18184 | | Position of W20_J4023_003_volume_map_bprime_local.mrc (#10) relative to |
| 18185 | | W20_J3623_consensus_structure.mrc (#1) coordinates: |
| 18186 | | Matrix rotation and translation |
| 18187 | | 0.99998445 0.00551741 0.00081396 -1.06075152 |
| 18188 | | -0.00551761 0.99998475 0.00023719 0.41218042 |
| 18189 | | -0.00081264 -0.00024168 0.99999964 0.27797318 |
| 18190 | | Axis -0.04289152 0.14569399 -0.98839950 |
| 18191 | | Axis point 78.63485140 193.79218176 0.00000000 |
| 18192 | | Rotation angle (degrees) 0.31984208 |
| 18193 | | Shift along axis -0.16919909 |
| 18194 | | |
| 18195 | | |
| 18196 | | > fitmap #11 inMap #1 |
| 18197 | | |
| 18198 | | Fit map cryosparc_P126_J4023_003_volume_map_half_A.mrc in map |
| 18199 | | W20_J3623_consensus_structure.mrc using 138113 points |
| 18200 | | correlation = 0.9179, correlation about mean = 0.2457, overlap = 1.663e+04 |
| 18201 | | steps = 52, shift = 0.377, angle = 0.301 degrees |
| 18202 | | |
| 18203 | | Position of cryosparc_P126_J4023_003_volume_map_half_A.mrc (#11) relative to |
| 18204 | | W20_J3623_consensus_structure.mrc (#1) coordinates: |
| 18205 | | Matrix rotation and translation |
| 18206 | | 0.99998625 0.00523529 0.00031389 -0.97109463 |
| 18207 | | -0.00523523 0.99998627 -0.00021220 0.44237207 |
| 18208 | | -0.00031500 0.00021056 0.99999993 0.12645487 |
| 18209 | | Axis 0.04027075 0.05990654 -0.99739133 |
| 18210 | | Axis point 85.60249694 184.64779847 0.00000000 |
| 18211 | | Rotation angle (degrees) 0.30074416 |
| 18212 | | Shift along axis -0.13873071 |
| 18213 | | |
| 18214 | | |
| 18215 | | > fitmap #11 inMap #1 |
| 18216 | | |
| 18217 | | Fit map cryosparc_P126_J4023_003_volume_map_half_A.mrc in map |
| 18218 | | W20_J3623_consensus_structure.mrc using 138113 points |
| 18219 | | correlation = 0.9179, correlation about mean = 0.2458, overlap = 1.664e+04 |
| 18220 | | steps = 28, shift = 0.0171, angle = 0.0164 degrees |
| 18221 | | |
| 18222 | | Position of cryosparc_P126_J4023_003_volume_map_half_A.mrc (#11) relative to |
| 18223 | | W20_J3623_consensus_structure.mrc (#1) coordinates: |
| 18224 | | Matrix rotation and translation |
| 18225 | | 0.99998502 0.00545441 0.00046410 -1.02505042 |
| 18226 | | -0.00545426 0.99998508 -0.00031696 0.50618634 |
| 18227 | | -0.00046583 0.00031443 0.99999984 0.13601178 |
| 18228 | | Axis 0.05757496 0.08479798 -0.99473334 |
| 18229 | | Axis point 94.64872752 186.59259606 0.00000000 |
| 18230 | | Rotation angle (degrees) 0.31416658 |
| 18231 | | Shift along axis -0.15138911 |
| 18232 | | |
| 18233 | | |
| 18234 | | > volume resample #11 #12 #10 onGrid #1 |
| 18235 | | |
| 18236 | | Opened cryosparc_P126_J4023_003_volume_map_half_A.mrc resampled as #13, grid |
| 18237 | | size 240,240,240, pixel 1.19, shown at step 1, values float32 |
| 18238 | | Opened cryosparc_P126_J4023_003_volume_map_half_B.mrc resampled as #14, grid |
| 18239 | | size 240,240,240, pixel 1.19, shown at step 1, values float32 |
| 18240 | | Opened W20_J4023_003_volume_map_bprime_local.mrc resampled as #15, grid size |
| 18241 | | 240,240,240, pixel 1.19, shown at step 1, values float32 |
| 18242 | | |
| 18243 | | > save W20_J4022_halfA_consensus_resampled.mrc #13 |
| 18244 | | |
| 18245 | | > save W20_J4022_halfB_consensus_resampled.mrc #14 |
| 18246 | | |
| 18247 | | > save W20_J4022_consensus_resampled.mrc #15 |
| 18248 | | |
| 18249 | | > close #10-15 |
| 18250 | | |
| 18251 | | > open "/Users/becca/Desktop/Postdoc/COPI notebooks and |
| 18252 | | > resources/Processing/W20_MM_cyto_COPI/W20_J4022_consensus_resampled.mrc" |
| 18253 | | |
| 18254 | | Opened W20_J4022_consensus_resampled.mrc as #10, grid size 240,240,240, pixel |
| 18255 | | 1.19, shown at level 0.256, step 1, values float32 |
| 18256 | | |
| 18257 | | > show #!8 models |
| 18258 | | |
| 18259 | | > hide #!10 models |
| 18260 | | |
| 18261 | | > show #!10 models |
| 18262 | | |
| 18263 | | > hide #!10 models |
| 18264 | | |
| 18265 | | > show #!10 models |
| 18266 | | |
| 18267 | | > hide #!10 models |
| 18268 | | |
| 18269 | | > show #!10 models |
| 18270 | | |
| 18271 | | > hide #!10 models |
| 18272 | | |
| 18273 | | > show #!10 models |
| 18274 | | |
| 18275 | | > hide #!10 models |
| 18276 | | |
| 18277 | | > close #8-10 |
| 18278 | | |
| 18279 | | > open "/Users/becca/Desktop/Postdoc/COPI notebooks and |
| 18280 | | > resources/Processing/W20_MM_cyto_COPI/W20_J4022_005_volume_map_bprime_local.mrc" |
| 18281 | | |
| 18282 | | Opened W20_J4022_005_volume_map_bprime_local.mrc as #8, grid size 240,240,240, |
| 18283 | | pixel 1.19, shown at level 0.256, step 1, values float32 |
| 18284 | | |
| 18285 | | > open "/Users/becca/Desktop/Postdoc/COPI notebooks and |
| 18286 | | > resources/Processing/W20_MM_cyto_COPI/cryosparc_P126_J4022_005_volume_map_half_A.mrc" |
| 18287 | | |
| 18288 | | Opened cryosparc_P126_J4022_005_volume_map_half_A.mrc as #9, grid size |
| 18289 | | 240,240,240, pixel 1.19, shown at level 0.259, step 1, values float32 |
| 18290 | | |
| 18291 | | > open "/Users/becca/Desktop/Postdoc/COPI notebooks and |
| 18292 | | > resources/Processing/W20_MM_cyto_COPI/cryosparc_P126_J4022_005_volume_map_half_B.mrc" |
| 18293 | | |
| 18294 | | Opened cryosparc_P126_J4022_005_volume_map_half_B.mrc as #10, grid size |
| 18295 | | 240,240,240, pixel 1.19, shown at level 0.257, step 1, values float32 |
| 18296 | | |
| 18297 | | > fitmap #8 inMap #1 |
| 18298 | | |
| 18299 | | Fit map W20_J4022_005_volume_map_bprime_local.mrc in map |
| 18300 | | W20_J3623_consensus_structure.mrc using 138200 points |
| 18301 | | correlation = 0.9195, correlation about mean = 0.2263, overlap = 1.667e+04 |
| 18302 | | steps = 64, shift = 0.329, angle = 0.376 degrees |
| 18303 | | |
| 18304 | | Position of W20_J4022_005_volume_map_bprime_local.mrc (#8) relative to |
| 18305 | | W20_J3623_consensus_structure.mrc (#1) coordinates: |
| 18306 | | Matrix rotation and translation |
| 18307 | | 0.99997966 0.00237299 0.00592048 -1.40324868 |
| 18308 | | -0.00238223 0.99999595 0.00155423 -0.17561136 |
| 18309 | | -0.00591677 -0.00156830 0.99998127 1.18884605 |
| 18310 | | Axis -0.23775685 0.90131776 -0.36207456 |
| 18311 | | Axis point 184.53246236 0.00000000 247.89429522 |
| 18312 | | Rotation angle (degrees) 0.37624341 |
| 18313 | | Shift along axis -0.25510056 |
| 18314 | | |
| 18315 | | |
| 18316 | | > fitmap #9 inMap #1 |
| 18317 | | |
| 18318 | | Fit map cryosparc_P126_J4022_005_volume_map_half_A.mrc in map |
| 18319 | | W20_J3623_consensus_structure.mrc using 138165 points |
| 18320 | | correlation = 0.9121, correlation about mean = 0.2433, overlap = 1.687e+04 |
| 18321 | | steps = 52, shift = 0.339, angle = 0.357 degrees |
| 18322 | | |
| 18323 | | Position of cryosparc_P126_J4022_005_volume_map_half_A.mrc (#9) relative to |
| 18324 | | W20_J3623_consensus_structure.mrc (#1) coordinates: |
| 18325 | | Matrix rotation and translation |
| 18326 | | 0.99998304 0.00160770 0.00559706 -1.23594018 |
| 18327 | | -0.00161999 0.99999628 0.00219278 -0.39387001 |
| 18328 | | -0.00559351 -0.00220181 0.99998193 1.22871953 |
| 18329 | | Axis -0.35302911 0.89896616 -0.25928803 |
| 18330 | | Axis point 207.94947596 0.00000000 236.35689026 |
| 18331 | | Rotation angle (degrees) 0.35661877 |
| 18332 | | Shift along axis -0.23634521 |
| 18333 | | |
| 18334 | | |
| 18335 | | > fitmap #10 inMap #1 |
| 18336 | | |
| 18337 | | Fit map cryosparc_P126_J4022_005_volume_map_half_B.mrc in map |
| 18338 | | W20_J3623_consensus_structure.mrc using 138076 points |
| 18339 | | correlation = 0.9128, correlation about mean = 0.2508, overlap = 1.688e+04 |
| 18340 | | steps = 64, shift = 0.335, angle = 0.335 degrees |
| 18341 | | |
| 18342 | | Position of cryosparc_P126_J4022_005_volume_map_half_B.mrc (#10) relative to |
| 18343 | | W20_J3623_consensus_structure.mrc (#1) coordinates: |
| 18344 | | Matrix rotation and translation |
| 18345 | | 0.99998354 0.00229208 0.00525924 -1.29178101 |
| 18346 | | -0.00229810 0.99999671 0.00113989 -0.13676617 |
| 18347 | | -0.00525661 -0.00115196 0.99998552 0.99455043 |
| 18348 | | Axis -0.19587427 0.89873969 -0.39230121 |
| 18349 | | Axis point 169.08755660 -0.00000000 255.83594172 |
| 18350 | | Rotation angle (degrees) 0.33520093 |
| 18351 | | Shift along axis -0.26005386 |
| 18352 | | |
| 18353 | | |
| 18354 | | > volume resample #8 #9 #10 onGrid #1 |
| 18355 | | |
| 18356 | | Opened W20_J4022_005_volume_map_bprime_local.mrc resampled as #11, grid size |
| 18357 | | 240,240,240, pixel 1.19, shown at step 1, values float32 |
| 18358 | | Opened cryosparc_P126_J4022_005_volume_map_half_A.mrc resampled as #12, grid |
| 18359 | | size 240,240,240, pixel 1.19, shown at step 1, values float32 |
| 18360 | | Opened cryosparc_P126_J4022_005_volume_map_half_B.mrc resampled as #13, grid |
| 18361 | | size 240,240,240, pixel 1.19, shown at step 1, values float32 |
| 18362 | | |
| 18363 | | > save W20_J4022_halfA_consensus_resampled.mrc #12 |
| 18364 | | |
| 18365 | | > save W20_J4022_halfb_consensus_resampled.mrc #12 |
| 18366 | | |
| 18367 | | > save W20_J4022_halfB_consensus_resampled.mrc #12 |
| 18368 | | |
| 18369 | | > save W20_J4022_halfB_consensus_resampled.mrc #13 |
| 18370 | | |
| 18371 | | > save W20_J4022_halfA_consensus_resampled.mrc #12 |
| 18372 | | |
| 18373 | | > save W20_J4022_consensus_resampled.mrc #11 |
| 18374 | | |
| 18375 | | > close #8-10 |
| 18376 | | |
| 18377 | | > open "/Users/becca/Desktop/Postdoc/COPI notebooks and |
| 18378 | | > resources/Processing/W20_MM_cyto_COPI/W20_J4022_consensus_resampled.mrc" |
| 18379 | | |
| 18380 | | Opened W20_J4022_consensus_resampled.mrc as #8, grid size 240,240,240, pixel |
| 18381 | | 1.19, shown at level 0.256, step 1, values float32 |
| 18382 | | |
| 18383 | | > open "/Users/becca/Desktop/Postdoc/COPI notebooks and |
| 18384 | | > resources/Processing/W20_MM_cyto_COPI/W20_J4022_halfA_consensus_resampled.mrc" |
| 18385 | | > "/Users/becca/Desktop/Postdoc/COPI notebooks and |
| 18386 | | > resources/Processing/W20_MM_cyto_COPI/W20_J4022_halfB_consensus_resampled.mrc" |
| 18387 | | |
| 18388 | | Opened W20_J4022_halfA_consensus_resampled.mrc as #9.1, grid size 240,240,240, |
| 18389 | | pixel 1.19, shown at level 0.257, step 1, values float32 |
| 18390 | | Opened W20_J4022_halfB_consensus_resampled.mrc as #9.2, grid size 240,240,240, |
| 18391 | | pixel 1.19, shown at level 0.255, step 1, values float32 |
| 18392 | | |
| 18393 | | > rename #9 J4022_half_maps |
| 18394 | | |
| 18395 | | > close #11-13 |
| 18396 | | |
| 18397 | | > hide #!9 models |
| 18398 | | |
| 18399 | | > hide #!8 models |
| 18400 | | |
| 18401 | | > open "/Users/becca/Desktop/Postdoc/COPI notebooks and |
| 18402 | | > resources/Processing/W20_MM_cyto_COPI/cryosparc_P126_J4023_003_volume_map_half_A.mrc" |
| 18403 | | |
| 18404 | | Opened cryosparc_P126_J4023_003_volume_map_half_A.mrc as #10, grid size |
| 18405 | | 240,240,240, pixel 1.19, shown at level 0.26, step 1, values float32 |
| 18406 | | |
| 18407 | | > open "/Users/becca/Desktop/Postdoc/COPI notebooks and |
| 18408 | | > resources/Processing/W20_MM_cyto_COPI/cryosparc_P126_J4023_003_volume_map_half_B.mrc" |
| 18409 | | |
| 18410 | | Opened cryosparc_P126_J4023_003_volume_map_half_B.mrc as #11, grid size |
| 18411 | | 240,240,240, pixel 1.19, shown at level 0.261, step 1, values float32 |
| 18412 | | |
| 18413 | | > open "/Users/becca/Desktop/Postdoc/COPI notebooks and |
| 18414 | | > resources/Processing/W20_MM_cyto_COPI/W20_J4023_003_volume_map_bprime_local.mrc" |
| 18415 | | |
| 18416 | | Opened W20_J4023_003_volume_map_bprime_local.mrc as #12, grid size |
| 18417 | | 240,240,240, pixel 1.19, shown at level 0.26, step 1, values float32 |
| 18418 | | |
| 18419 | | > fitmap #10 inMap #1 |
| 18420 | | |
| 18421 | | Fit map cryosparc_P126_J4023_003_volume_map_half_A.mrc in map |
| 18422 | | W20_J3623_consensus_structure.mrc using 138113 points |
| 18423 | | correlation = 0.9179, correlation about mean = 0.2457, overlap = 1.663e+04 |
| 18424 | | steps = 52, shift = 0.377, angle = 0.301 degrees |
| 18425 | | |
| 18426 | | Position of cryosparc_P126_J4023_003_volume_map_half_A.mrc (#10) relative to |
| 18427 | | W20_J3623_consensus_structure.mrc (#1) coordinates: |
| 18428 | | Matrix rotation and translation |
| 18429 | | 0.99998625 0.00523529 0.00031389 -0.97109463 |
| 18430 | | -0.00523523 0.99998627 -0.00021220 0.44237207 |
| 18431 | | -0.00031500 0.00021056 0.99999993 0.12645487 |
| 18432 | | Axis 0.04027075 0.05990654 -0.99739133 |
| 18433 | | Axis point 85.60249694 184.64779847 0.00000000 |
| 18434 | | Rotation angle (degrees) 0.30074416 |
| 18435 | | Shift along axis -0.13873071 |
| 18436 | | |
| 18437 | | |
| 18438 | | > fitmap #11 inMap #1 |
| 18439 | | |
| 18440 | | Fit map cryosparc_P126_J4023_003_volume_map_half_B.mrc in map |
| 18441 | | W20_J3623_consensus_structure.mrc using 138029 points |
| 18442 | | correlation = 0.9185, correlation about mean = 0.2483, overlap = 1.668e+04 |
| 18443 | | steps = 68, shift = 0.416, angle = 0.319 degrees |
| 18444 | | |
| 18445 | | Position of cryosparc_P126_J4023_003_volume_map_half_B.mrc (#11) relative to |
| 18446 | | W20_J3623_consensus_structure.mrc (#1) coordinates: |
| 18447 | | Matrix rotation and translation |
| 18448 | | 0.99998492 0.00536357 0.00117882 -1.09568335 |
| 18449 | | -0.00536466 0.99998519 0.00091977 0.25675329 |
| 18450 | | -0.00117387 -0.00092608 0.99999888 0.47324489 |
| 18451 | | Axis -0.16573766 0.21124575 -0.96327891 |
| 18452 | | Axis point 55.94119463 211.23890617 0.00000000 |
| 18453 | | Rotation angle (degrees) 0.31905878 |
| 18454 | | Shift along axis -0.22003278 |
| 18455 | | |
| 18456 | | |
| 18457 | | > fitmap #12 inMap #1 |
| 18458 | | |
| 18459 | | Fit map W20_J4023_003_volume_map_bprime_local.mrc in map |
| 18460 | | W20_J3623_consensus_structure.mrc using 138098 points |
| 18461 | | correlation = 0.9217, correlation about mean = 0.2228, overlap = 1.65e+04 |
| 18462 | | steps = 52, shift = 0.377, angle = 0.32 degrees |
| 18463 | | |
| 18464 | | Position of W20_J4023_003_volume_map_bprime_local.mrc (#12) relative to |
| 18465 | | W20_J3623_consensus_structure.mrc (#1) coordinates: |
| 18466 | | Matrix rotation and translation |
| 18467 | | 0.99998445 0.00551741 0.00081396 -1.06075152 |
| 18468 | | -0.00551761 0.99998475 0.00023719 0.41218042 |
| 18469 | | -0.00081264 -0.00024168 0.99999964 0.27797318 |
| 18470 | | Axis -0.04289152 0.14569399 -0.98839950 |
| 18471 | | Axis point 78.63485140 193.79218176 0.00000000 |
| 18472 | | Rotation angle (degrees) 0.31984208 |
| 18473 | | Shift along axis -0.16919909 |
| 18474 | | |
| 18475 | | |
| 18476 | | > volume resample #11 #12 #10 onGrid #1 |
| 18477 | | |
| 18478 | | Opened cryosparc_P126_J4023_003_volume_map_half_B.mrc resampled as #13, grid |
| 18479 | | size 240,240,240, pixel 1.19, shown at step 1, values float32 |
| 18480 | | Opened W20_J4023_003_volume_map_bprime_local.mrc resampled as #14, grid size |
| 18481 | | 240,240,240, pixel 1.19, shown at step 1, values float32 |
| 18482 | | Opened cryosparc_P126_J4023_003_volume_map_half_A.mrc resampled as #15, grid |
| 18483 | | size 240,240,240, pixel 1.19, shown at step 1, values float32 |
| 18484 | | |
| 18485 | | > save W20_J4023_halfA_consensus_resampled.mrc #15 |
| 18486 | | |
| 18487 | | > save W20_J4023_halfB_consensus_resampled.mrc #13 |
| 18488 | | |
| 18489 | | > save W20_J4023_consensus_resampled.mrc #14 |
| 18490 | | |
| 18491 | | > close #10-12 |
| 18492 | | |
| 18493 | | > open "/Users/becca/Desktop/Postdoc/COPI notebooks and |
| 18494 | | > resources/Processing/W20_MM_cyto_COPI/W20_J4023_consensus_resampled.mrc" |
| 18495 | | |
| 18496 | | Opened W20_J4023_consensus_resampled.mrc as #10, grid size 240,240,240, pixel |
| 18497 | | 1.19, shown at level 0.259, step 1, values float32 |
| 18498 | | |
| 18499 | | > open "/Users/becca/Desktop/Postdoc/COPI notebooks and |
| 18500 | | > resources/Processing/W20_MM_cyto_COPI/W20_J4023_halfA_consensus_resampled.mrc" |
| 18501 | | > "/Users/becca/Desktop/Postdoc/COPI notebooks and |
| 18502 | | > resources/Processing/W20_MM_cyto_COPI/W20_J4023_halfB_consensus_resampled.mrc" |
| 18503 | | |
| 18504 | | Opened W20_J4023_halfA_consensus_resampled.mrc as #11.1, grid size |
| 18505 | | 240,240,240, pixel 1.19, shown at level 0.258, step 1, values float32 |
| 18506 | | Opened W20_J4023_halfB_consensus_resampled.mrc as #11.2, grid size |
| 18507 | | 240,240,240, pixel 1.19, shown at level 0.259, step 1, values float32 |
| 18508 | | |
| 18509 | | > rename #11 J4023_half_maps |
| 18510 | | |
| 18511 | | > close #13-15 |
| 18512 | | |
| 18513 | | > open "/Users/becca/Desktop/Postdoc/COPI notebooks and |
| 18514 | | > resources/Processing/W20_MM_cyto_COPI/cryosparc_P126_J4024_005_volume_map_half_B.mrc" |
| 18515 | | |
| 18516 | | Opened cryosparc_P126_J4024_005_volume_map_half_B.mrc as #12, grid size |
| 18517 | | 240,240,240, pixel 1.19, shown at level 0.258, step 1, values float32 |
| 18518 | | |
| 18519 | | > open "/Users/becca/Desktop/Postdoc/COPI notebooks and |
| 18520 | | > resources/Processing/W20_MM_cyto_COPI/cryosparc_P126_J4024_005_volume_map_half_A.mrc" |
| 18521 | | |
| 18522 | | Opened cryosparc_P126_J4024_005_volume_map_half_A.mrc as #13, grid size |
| 18523 | | 240,240,240, pixel 1.19, shown at level 0.259, step 1, values float32 |
| 18524 | | |
| 18525 | | > open "/Users/becca/Desktop/Postdoc/COPI notebooks and |
| 18526 | | > resources/Processing/W20_MM_cyto_COPI/W20_J4024_005_volume_map_bprime_local.mrc" |
| 18527 | | |
| 18528 | | Opened W20_J4024_005_volume_map_bprime_local.mrc as #14, grid size |
| 18529 | | 240,240,240, pixel 1.19, shown at level 0.256, step 1, values float32 |
| 18530 | | |
| 18531 | | > hide #!10 models |
| 18532 | | |
| 18533 | | > hide #!11 models |
| 18534 | | |
| 18535 | | > fitmap #12 inMap #1 |
| 18536 | | |
| 18537 | | Fit map cryosparc_P126_J4024_005_volume_map_half_B.mrc in map |
| 18538 | | W20_J3623_consensus_structure.mrc using 138074 points |
| 18539 | | correlation = 0.9152, correlation about mean = 0.3069, overlap = 1.694e+04 |
| 18540 | | steps = 48, shift = 0.275, angle = 0.182 degrees |
| 18541 | | |
| 18542 | | Position of cryosparc_P126_J4024_005_volume_map_half_B.mrc (#12) relative to |
| 18543 | | W20_J3623_consensus_structure.mrc (#1) coordinates: |
| 18544 | | Matrix rotation and translation |
| 18545 | | 0.99999497 0.00227004 0.00221345 -0.56219185 |
| 18546 | | -0.00226948 0.99999739 -0.00025704 0.14204421 |
| 18547 | | -0.00221402 0.00025201 0.99999752 0.19043665 |
| 18548 | | Axis 0.08002006 0.69597786 -0.71359065 |
| 18549 | | Axis point 87.46071279 244.95972740 0.00000000 |
| 18550 | | Rotation angle (degrees) 0.18224416 |
| 18551 | | Shift along axis -0.08202081 |
| 18552 | | |
| 18553 | | |
| 18554 | | > fitmap #13 inMap #1 |
| 18555 | | |
| 18556 | | Fit map cryosparc_P126_J4024_005_volume_map_half_A.mrc in map |
| 18557 | | W20_J3623_consensus_structure.mrc using 138160 points |
| 18558 | | correlation = 0.9177, correlation about mean = 0.3098, overlap = 1.706e+04 |
| 18559 | | steps = 52, shift = 0.291, angle = 0.229 degrees |
| 18560 | | |
| 18561 | | Position of cryosparc_P126_J4024_005_volume_map_half_A.mrc (#13) relative to |
| 18562 | | W20_J3623_consensus_structure.mrc (#1) coordinates: |
| 18563 | | Matrix rotation and translation |
| 18564 | | 0.99999333 0.00148868 0.00333575 -0.58679763 |
| 18565 | | -0.00149415 0.99999754 0.00163794 -0.27610320 |
| 18566 | | -0.00333331 -0.00164291 0.99999309 0.70324776 |
| 18567 | | Axis -0.40966600 0.83273727 -0.37245458 |
| 18568 | | Axis point 182.44969494 0.00000000 207.15832332 |
| 18569 | | Rotation angle (degrees) 0.22943002 |
| 18570 | | Shift along axis -0.25145824 |
| 18571 | | |
| 18572 | | |
| 18573 | | > fitmap #14 inMap #1 |
| 18574 | | |
| 18575 | | Fit map W20_J4024_005_volume_map_bprime_local.mrc in map |
| 18576 | | W20_J3623_consensus_structure.mrc using 138193 points |
| 18577 | | correlation = 0.9249, correlation about mean = 0.3066, overlap = 1.679e+04 |
| 18578 | | steps = 64, shift = 0.289, angle = 0.207 degrees |
| 18579 | | |
| 18580 | | Position of W20_J4024_005_volume_map_bprime_local.mrc (#14) relative to |
| 18581 | | W20_J3623_consensus_structure.mrc (#1) coordinates: |
| 18582 | | Matrix rotation and translation |
| 18583 | | 0.99999384 0.00210499 0.00280839 -0.61107104 |
| 18584 | | -0.00210740 0.99999741 0.00085537 -0.06363233 |
| 18585 | | -0.00280658 -0.00086128 0.99999569 0.47576775 |
| 18586 | | Axis -0.23755676 0.77702082 -0.58292833 |
| 18587 | | Axis point 131.43847496 0.00000000 233.23904124 |
| 18588 | | Rotation angle (degrees) 0.20701789 |
| 18589 | | Shift along axis -0.18161809 |
| 18590 | | |
| 18591 | | |
| 18592 | | > volume resample #13 #12 #14 onGrid #1 |
| 18593 | | |
| 18594 | | Opened cryosparc_P126_J4024_005_volume_map_half_A.mrc resampled as #15, grid |
| 18595 | | size 240,240,240, pixel 1.19, shown at step 1, values float32 |
| 18596 | | Opened cryosparc_P126_J4024_005_volume_map_half_B.mrc resampled as #16, grid |
| 18597 | | size 240,240,240, pixel 1.19, shown at step 1, values float32 |
| 18598 | | Opened W20_J4024_005_volume_map_bprime_local.mrc resampled as #17, grid size |
| 18599 | | 240,240,240, pixel 1.19, shown at step 1, values float32 |
| 18600 | | |
| 18601 | | > save W20_J4024_halfA_consensus_resampled.mrc #15 |
| 18602 | | |
| 18603 | | > save W20_J4024_halfB_consensus_resampled.mrc #16 |
| 18604 | | |
| 18605 | | > save W20_J4024_consensus_resampled.mrc #17 |
| 18606 | | |
| 18607 | | > combine #18 |
| 18608 | | |
| 18609 | | > hide #!28 models |
| 18610 | | |
| 18611 | | > close #12-14 |
| 18612 | | |
| 18613 | | > open "/Users/becca/Desktop/Postdoc/COPI notebooks and |
| 18614 | | > resources/Processing/W20_MM_cyto_COPI/W20_J4024_consensus_resampled.mrc" |
| 18615 | | |
| 18616 | | Opened W20_J4024_consensus_resampled.mrc as #12, grid size 240,240,240, pixel |
| 18617 | | 1.19, shown at level 0.256, step 1, values float32 |
| 18618 | | |
| 18619 | | > open "/Users/becca/Desktop/Postdoc/COPI notebooks and |
| 18620 | | > resources/Processing/W20_MM_cyto_COPI/W20_J4024_halfA_consensus_resampled.mrc" |
| 18621 | | > "/Users/becca/Desktop/Postdoc/COPI notebooks and |
| 18622 | | > resources/Processing/W20_MM_cyto_COPI/W20_J4024_halfB_consensus_resampled.mrc" |
| 18623 | | |
| 18624 | | Opened W20_J4024_halfA_consensus_resampled.mrc as #13.1, grid size |
| 18625 | | 240,240,240, pixel 1.19, shown at level 0.257, step 1, values float32 |
| 18626 | | Opened W20_J4024_halfB_consensus_resampled.mrc as #13.2, grid size |
| 18627 | | 240,240,240, pixel 1.19, shown at level 0.257, step 1, values float32 |
| 18628 | | |
| 18629 | | > rename #13 J4024_half_maps |
| 18630 | | |
| 18631 | | > hide #!13 models |
| 18632 | | |
| 18633 | | > hide #!12 models |
| 18634 | | |
| 18635 | | > close #15-17 |
| 18636 | | |
| 18637 | | > open "/Users/becca/Desktop/Postdoc/COPI notebooks and |
| 18638 | | > resources/Processing/W20_MM_cyto_COPI/W20_J4025_004_volume_map_bprime_local.mrc" |
| 18639 | | |
| 18640 | | Opened W20_J4025_004_volume_map_bprime_local.mrc as #14, grid size |
| 18641 | | 240,240,240, pixel 1.19, shown at level 0.261, step 1, values float32 |
| 18642 | | |
| 18643 | | > open "/Users/becca/Desktop/Postdoc/COPI notebooks and |
| 18644 | | > resources/Processing/W20_MM_cyto_COPI/cryosparc_P126_J4025_004_volume_map_half_A.mrc" |
| 18645 | | |
| 18646 | | Opened cryosparc_P126_J4025_004_volume_map_half_A.mrc as #15, grid size |
| 18647 | | 240,240,240, pixel 1.19, shown at level 0.261, step 1, values float32 |
| 18648 | | |
| 18649 | | > open "/Users/becca/Desktop/Postdoc/COPI notebooks and |
| 18650 | | > resources/Processing/W20_MM_cyto_COPI/cryosparc_P126_J4025_004_volume_map_half_B.mrc" |
| 18651 | | |
| 18652 | | Opened cryosparc_P126_J4025_004_volume_map_half_B.mrc as #16, grid size |
| 18653 | | 240,240,240, pixel 1.19, shown at level 0.262, step 1, values float32 |
| 18654 | | |
| 18655 | | > fitmap #14 inMap #1 |
| 18656 | | |
| 18657 | | Fit map W20_J4025_004_volume_map_bprime_local.mrc in map |
| 18658 | | W20_J3623_consensus_structure.mrc using 138084 points |
| 18659 | | correlation = 0.9112, correlation about mean = 0.1801, overlap = 1.617e+04 |
| 18660 | | steps = 56, shift = 0.729, angle = 0.309 degrees |
| 18661 | | |
| 18662 | | Position of W20_J4025_004_volume_map_bprime_local.mrc (#14) relative to |
| 18663 | | W20_J3623_consensus_structure.mrc (#1) coordinates: |
| 18664 | | Matrix rotation and translation |
| 18665 | | 0.99999126 0.00417664 -0.00019252 -0.45232897 |
| 18666 | | -0.00417727 0.99998545 -0.00341415 0.60739960 |
| 18667 | | 0.00017826 0.00341492 0.99999415 -0.09374688 |
| 18668 | | Axis 0.63253451 -0.03434313 -0.77377041 |
| 18669 | | Axis point 143.22381791 73.09529688 0.00000000 |
| 18670 | | Rotation angle (degrees) 0.30929457 |
| 18671 | | Shift along axis -0.23443512 |
| 18672 | | |
| 18673 | | |
| 18674 | | > fitmap #15 inMap #1 |
| 18675 | | |
| 18676 | | Fit map cryosparc_P126_J4025_004_volume_map_half_A.mrc in map |
| 18677 | | W20_J3623_consensus_structure.mrc using 138098 points |
| 18678 | | correlation = 0.9052, correlation about mean = 0.2004, overlap = 1.632e+04 |
| 18679 | | steps = 56, shift = 0.719, angle = 0.323 degrees |
| 18680 | | |
| 18681 | | Position of cryosparc_P126_J4025_004_volume_map_half_A.mrc (#15) relative to |
| 18682 | | W20_J3623_consensus_structure.mrc (#1) coordinates: |
| 18683 | | Matrix rotation and translation |
| 18684 | | 0.99998806 0.00478731 -0.00097621 -0.44930386 |
| 18685 | | -0.00479005 0.99998454 -0.00282388 0.63846920 |
| 18686 | | 0.00096268 0.00282852 0.99999554 -0.08426557 |
| 18687 | | Axis 0.50071333 -0.17175476 -0.84840230 |
| 18688 | | Axis point 123.64037299 66.63895829 0.00000000 |
| 18689 | | Rotation angle (degrees) 0.32339898 |
| 18690 | | Shift along axis -0.26314146 |
| 18691 | | |
| 18692 | | |
| 18693 | | > fitmap #16 inMap #1 |
| 18694 | | |
| 18695 | | Fit map cryosparc_P126_J4025_004_volume_map_half_B.mrc in map |
| 18696 | | W20_J3623_consensus_structure.mrc using 138173 points |
| 18697 | | correlation = 0.9079, correlation about mean = 0.2131, overlap = 1.642e+04 |
| 18698 | | steps = 52, shift = 0.742, angle = 0.262 degrees |
| 18699 | | |
| 18700 | | Position of cryosparc_P126_J4025_004_volume_map_half_B.mrc (#16) relative to |
| 18701 | | W20_J3623_consensus_structure.mrc (#1) coordinates: |
| 18702 | | Matrix rotation and translation |
| 18703 | | 0.99999486 0.00315412 0.00057190 -0.38968890 |
| 18704 | | -0.00315224 0.99998971 -0.00326115 0.40412911 |
| 18705 | | -0.00058218 0.00325933 0.99999452 0.02235804 |
| 18706 | | Axis 0.71306302 0.12620638 -0.68964707 |
| 18707 | | Axis point 0.00000000 44.63041208 133.15812471 |
| 18708 | | Rotation angle (degrees) 0.26196640 |
| 18709 | | Shift along axis -0.24228822 |
| 18710 | | |
| 18711 | | |
| 18712 | | > volume resample #15 #16 #14 onGrid #1 |
| 18713 | | |
| 18714 | | Opened cryosparc_P126_J4025_004_volume_map_half_A.mrc resampled as #17, grid |
| 18715 | | size 240,240,240, pixel 1.19, shown at step 1, values float32 |
| 18716 | | Opened cryosparc_P126_J4025_004_volume_map_half_B.mrc resampled as #41, grid |
| 18717 | | size 240,240,240, pixel 1.19, shown at step 1, values float32 |
| 18718 | | Opened W20_J4025_004_volume_map_bprime_local.mrc resampled as #90, grid size |
| 18719 | | 240,240,240, pixel 1.19, shown at step 1, values float32 |
| 18720 | | |
| 18721 | | > save W20_J4024_halfA_consensus_resampled.mrc #17 |
| 18722 | | |
| 18723 | | > hide #!13.2 models |
| 18724 | | |
| 18725 | | > hide #!13.1 models |
| 18726 | | |
| 18727 | | > show #!13.1 models |
| 18728 | | |
| 18729 | | > hide #!13.1 models |
| 18730 | | |
| 18731 | | > hide #!13 models |
| 18732 | | |
| 18733 | | > hide #!41 models |
| 18734 | | |
| 18735 | | > hide #!90 models |
| 18736 | | |
| 18737 | | > show #!13.1 models |
| 18738 | | |
| 18739 | | > hide #!13.1 models |
| 18740 | | |
| 18741 | | > show #!13.1 models |
| 18742 | | |
| 18743 | | > hide #!13.1 models |
| 18744 | | |
| 18745 | | > show #!13.1 models |
| 18746 | | |
| 18747 | | > hide #!13.1 models |
| 18748 | | |
| 18749 | | > show #!13.2 models |
| 18750 | | |
| 18751 | | > show #!13.1 models |
| 18752 | | |
| 18753 | | > hide #!13.1 models |
| 18754 | | |
| 18755 | | > hide #!13.2 models |
| 18756 | | |
| 18757 | | > save W20_J4024_halfA_consensus_resampled.mrc #13.1 |
| 18758 | | |
| 18759 | | > save W20_J4025_halfA_consensus_resampled.mrc #17 |
| 18760 | | |
| 18761 | | > rename #17 W20_J4025_halfA_consensus_resampled.mrc |
| 18762 | | |
| 18763 | | > show #!90 models |
| 18764 | | |
| 18765 | | > show #!41 models |
| 18766 | | |
| 18767 | | > save W20_J4025_halfB_consensus_resampled.mrc #41 |
| 18768 | | |
| 18769 | | > save W20_J4025_consensus_resampled.mrc #90 |
| 18770 | | |
| 18771 | | > hide #!13 models |
| 18772 | | |
| 18773 | | > close #14-16 |
| 18774 | | |
| 18775 | | > open "/Users/becca/Desktop/Postdoc/COPI notebooks and |
| 18776 | | > resources/Processing/W20_MM_cyto_COPI/W20_J4025_consensus_resampled.mrc" |
| 18777 | | |
| 18778 | | Opened W20_J4025_consensus_resampled.mrc as #14, grid size 240,240,240, pixel |
| 18779 | | 1.19, shown at level 0.26, step 1, values float32 |
| 18780 | | |
| 18781 | | > hide #!17 models |
| 18782 | | |
| 18783 | | > open "/Users/becca/Desktop/Postdoc/COPI notebooks and |
| 18784 | | > resources/Processing/W20_MM_cyto_COPI/W20_J4025_halfA_consensus_resampled.mrc" |
| 18785 | | > "/Users/becca/Desktop/Postdoc/COPI notebooks and |
| 18786 | | > resources/Processing/W20_MM_cyto_COPI/W20_J4025_halfB_consensus_resampled.mrc" |
| 18787 | | |
| 18788 | | Opened W20_J4025_halfA_consensus_resampled.mrc as #15.1, grid size |
| 18789 | | 240,240,240, pixel 1.19, shown at level 0.26, step 1, values float32 |
| 18790 | | Opened W20_J4025_halfB_consensus_resampled.mrc as #15.2, grid size |
| 18791 | | 240,240,240, pixel 1.19, shown at level 0.26, step 1, values float32 |
| 18792 | | |
| 18793 | | > save W20_J4024_halfA_consensus_resampled.mrc #13.1 |
| 18794 | | |
| 18795 | | > rename #15 J4025_half_maps |
| 18796 | | |
| 18797 | | > hide #!15 models |
| 18798 | | |
| 18799 | | > hide #!14 models |
| 18800 | | |
| 18801 | | > close #17 |
| 18802 | | |
| 18803 | | > close #18 |
| 18804 | | |
| 18805 | | > close #19 |
| 18806 | | |
| 18807 | | > close #20 |
| 18808 | | |
| 18809 | | > close #21 |
| 18810 | | |
| 18811 | | > close #22 |
| 18812 | | |
| 18813 | | > close #23 |
| 18814 | | |
| 18815 | | > hide #!1 models |
| 18816 | | |
| 18817 | | > show #!1 models |
| 18818 | | |
| 18819 | | > hide #!1 models |
| 18820 | | |
| 18821 | | > show #!1 models |
| 18822 | | |
| 18823 | | > hide #!1 models |
| 18824 | | |
| 18825 | | > show #!2 models |
| 18826 | | |
| 18827 | | > hide #!2 models |
| 18828 | | |
| 18829 | | > show #!2 models |
| 18830 | | |
| 18831 | | > hide #!2 models |
| 18832 | | |
| 18833 | | > show #!3 models |
| 18834 | | |
| 18835 | | > hide #!3 models |
| 18836 | | |
| 18837 | | > open "/Users/becca/Desktop/Postdoc/COPI notebooks and |
| 18838 | | > resources/Processing/W20_MM_cyto_COPI/cryosparc_P126_J4026_004_volume_map_half_A.mrc" |
| 18839 | | |
| 18840 | | Opened cryosparc_P126_J4026_004_volume_map_half_A.mrc as #16, grid size |
| 18841 | | 240,240,240, pixel 1.19, shown at level 0.266, step 1, values float32 |
| 18842 | | |
| 18843 | | > open "/Users/becca/Desktop/Postdoc/COPI notebooks and |
| 18844 | | > resources/Processing/W20_MM_cyto_COPI/cryosparc_P126_J4026_004_volume_map_half_B.mrc" |
| 18845 | | |
| 18846 | | Opened cryosparc_P126_J4026_004_volume_map_half_B.mrc as #17, grid size |
| 18847 | | 240,240,240, pixel 1.19, shown at level 0.265, step 1, values float32 |
| 18848 | | |
| 18849 | | > open "/Users/becca/Desktop/Postdoc/COPI notebooks and |
| 18850 | | > resources/Processing/W20_MM_cyto_COPI/W20_J4026_004_volume_map_gammazeta_local.mrc" |
| 18851 | | |
| 18852 | | Opened W20_J4026_004_volume_map_gammazeta_local.mrc as #18, grid size |
| 18853 | | 240,240,240, pixel 1.19, shown at level 0.26, step 1, values float32 |
| 18854 | | |
| 18855 | | > hide #!17 models |
| 18856 | | |
| 18857 | | > show #!17 models |
| 18858 | | |
| 18859 | | > hide #!17 models |
| 18860 | | |
| 18861 | | > show #!17 models |
| 18862 | | |
| 18863 | | > hide #!18 models |
| 18864 | | |
| 18865 | | > hide #!41 models |
| 18866 | | |
| 18867 | | > hide #!90 models |
| 18868 | | |
| 18869 | | > hide #!17 models |
| 18870 | | |
| 18871 | | > show #!17 models |
| 18872 | | |
| 18873 | | > hide #!17 models |
| 18874 | | |
| 18875 | | > hide #!16 models |
| 18876 | | |
| 18877 | | > show #!18 models |
| 18878 | | |
| 18879 | | > hide #!18 models |
| 18880 | | |
| 18881 | | > show #!18 models |
| 18882 | | |
| 18883 | | > show #!17 models |
| 18884 | | |
| 18885 | | > hide #!17 models |
| 18886 | | |
| 18887 | | > show #!16 models |
| 18888 | | |
| 18889 | | > hide #!16 models |
| 18890 | | |
| 18891 | | > show #!16 models |
| 18892 | | |
| 18893 | | > show #!17 models |
| 18894 | | |
| 18895 | | > hide #!16 models |
| 18896 | | |
| 18897 | | > show #!16 models |
| 18898 | | |
| 18899 | | > hide #!17 models |
| 18900 | | |
| 18901 | | > show #!17 models |
| 18902 | | |
| 18903 | | > fitmap #16 inMap #1 |
| 18904 | | |
| 18905 | | Fit map cryosparc_P126_J4026_004_volume_map_half_A.mrc in map |
| 18906 | | W20_J3623_consensus_structure.mrc using 138044 points |
| 18907 | | correlation = 0.9061, correlation about mean = 0.2969, overlap = 1.877e+04 |
| 18908 | | steps = 52, shift = 0.633, angle = 0.336 degrees |
| 18909 | | |
| 18910 | | Position of cryosparc_P126_J4026_004_volume_map_half_A.mrc (#16) relative to |
| 18911 | | W20_J3623_consensus_structure.mrc (#1) coordinates: |
| 18912 | | Matrix rotation and translation |
| 18913 | | 0.99999262 0.00086300 -0.00374493 -0.00440355 |
| 18914 | | -0.00084638 0.99998980 0.00443601 -0.04656357 |
| 18915 | | 0.00374872 -0.00443280 0.99998315 0.35139305 |
| 18916 | | Axis -0.75569566 -0.63852116 -0.14565300 |
| 18917 | | Axis point 0.00000000 78.62510597 13.28594619 |
| 18918 | | Rotation angle (degrees) 0.33621225 |
| 18919 | | Shift along axis -0.01812188 |
| 18920 | | |
| 18921 | | |
| 18922 | | > fitmap #17 inMap #1 |
| 18923 | | |
| 18924 | | Fit map cryosparc_P126_J4026_004_volume_map_half_B.mrc in map |
| 18925 | | W20_J3623_consensus_structure.mrc using 138078 points |
| 18926 | | correlation = 0.906, correlation about mean = 0.2947, overlap = 1.874e+04 |
| 18927 | | steps = 56, shift = 0.632, angle = 0.326 degrees |
| 18928 | | |
| 18929 | | Position of cryosparc_P126_J4026_004_volume_map_half_B.mrc (#17) relative to |
| 18930 | | W20_J3623_consensus_structure.mrc (#1) coordinates: |
| 18931 | | Matrix rotation and translation |
| 18932 | | 0.99999516 0.00097527 -0.00295606 -0.10060790 |
| 18933 | | -0.00096120 0.99998823 0.00475526 -0.05419298 |
| 18934 | | 0.00296066 -0.00475240 0.99998432 0.51671634 |
| 18935 | | Axis -0.83660673 -0.52062903 -0.17039538 |
| 18936 | | Axis point 0.00000000 109.57038951 9.00298594 |
| 18937 | | Rotation angle (degrees) 0.32557225 |
| 18938 | | Shift along axis 0.02433761 |
| 18939 | | |
| 18940 | | |
| 18941 | | > fitmap #18 inMap #1 |
| 18942 | | |
| 18943 | | Fit map W20_J4026_004_volume_map_gammazeta_local.mrc in map |
| 18944 | | W20_J3623_consensus_structure.mrc using 138172 points |
| 18945 | | correlation = 0.917, correlation about mean = 0.2905, overlap = 1.854e+04 |
| 18946 | | steps = 52, shift = 0.635, angle = 0.343 degrees |
| 18947 | | |
| 18948 | | Position of W20_J4026_004_volume_map_gammazeta_local.mrc (#18) relative to |
| 18949 | | W20_J3623_consensus_structure.mrc (#1) coordinates: |
| 18950 | | Matrix rotation and translation |
| 18951 | | 0.99999273 0.00094755 -0.00369338 -0.01379933 |
| 18952 | | -0.00093047 0.99998888 0.00462325 -0.05119869 |
| 18953 | | 0.00369772 -0.00461978 0.99998249 0.38867466 |
| 18954 | | Axis -0.77135465 -0.61680564 -0.15672526 |
| 18955 | | Axis point 0.00000000 83.44645675 13.76857176 |
| 18956 | | Rotation angle (degrees) 0.34328547 |
| 18957 | | Shift along axis -0.01869132 |
| 18958 | | |
| 18959 | | |
| 18960 | | > volume resample #17 #16 #18 onGrid #1 |
| 18961 | | |
| 18962 | | Opened cryosparc_P126_J4026_004_volume_map_half_B.mrc resampled as #19, grid |
| 18963 | | size 240,240,240, pixel 1.19, shown at step 1, values float32 |
| 18964 | | Opened cryosparc_P126_J4026_004_volume_map_half_A.mrc resampled as #20, grid |
| 18965 | | size 240,240,240, pixel 1.19, shown at step 1, values float32 |
| 18966 | | Opened W20_J4026_004_volume_map_gammazeta_local.mrc resampled as #21, grid |
| 18967 | | size 240,240,240, pixel 1.19, shown at step 1, values float32 |
| 18968 | | |
| 18969 | | > save W20_J4026_halfA_consensus_resampled.mrc #20 |
| 18970 | | |
| 18971 | | > save W20_J4026_halfB_consensus_resampled.mrc #19 |
| 18972 | | |
| 18973 | | > save W20_J4026_consensus_resampled.mrc #21 |
| 18974 | | |
| 18975 | | > close #16-18 |
| 18976 | | |
| 18977 | | > open "/Users/becca/Desktop/Postdoc/COPI notebooks and |
| 18978 | | > resources/Processing/W20_MM_cyto_COPI/W20_J4026_consensus_resampled.mrc" |
| 18979 | | |
| 18980 | | Opened W20_J4026_consensus_resampled.mrc as #16, grid size 240,240,240, pixel |
| 18981 | | 1.19, shown at level 0.26, step 1, values float32 |
| 18982 | | |
| 18983 | | > hide #!21 models |
| 18984 | | |
| 18985 | | > hide #!20 models |
| 18986 | | |
| 18987 | | > hide #!19 models |
| 18988 | | |
| 18989 | | > open "/Users/becca/Desktop/Postdoc/COPI notebooks and |
| 18990 | | > resources/Processing/W20_MM_cyto_COPI/W20_J4026_halfA_consensus_resampled.mrc" |
| 18991 | | > "/Users/becca/Desktop/Postdoc/COPI notebooks and |
| 18992 | | > resources/Processing/W20_MM_cyto_COPI/W20_J4026_halfB_consensus_resampled.mrc" |
| 18993 | | |
| 18994 | | Opened W20_J4026_halfA_consensus_resampled.mrc as #17.1, grid size |
| 18995 | | 240,240,240, pixel 1.19, shown at level 0.261, step 1, values float32 |
| 18996 | | Opened W20_J4026_halfB_consensus_resampled.mrc as #17.2, grid size |
| 18997 | | 240,240,240, pixel 1.19, shown at level 0.26, step 1, values float32 |
| 18998 | | |
| 18999 | | > rename #17 J4026_half_maps |
| 19000 | | |
| 19001 | | > close #19-21 |
| 19002 | | |
| 19003 | | > open "/Users/becca/Desktop/Postdoc/COPI notebooks and |
| 19004 | | > resources/Processing/W20_MM_cyto_COPI/cryosparc_P126_J4027_005_volume_map_half_A.mrc" |
| 19005 | | |
| 19006 | | Opened cryosparc_P126_J4027_005_volume_map_half_A.mrc as #18, grid size |
| 19007 | | 240,240,240, pixel 1.19, shown at level 0.26, step 1, values float32 |
| 19008 | | |
| 19009 | | > open "/Users/becca/Desktop/Postdoc/COPI notebooks and |
| 19010 | | > resources/Processing/W20_MM_cyto_COPI/cryosparc_P126_J4027_005_volume_map_half_B.mrc" |
| 19011 | | |
| 19012 | | Opened cryosparc_P126_J4027_005_volume_map_half_B.mrc as #19, grid size |
| 19013 | | 240,240,240, pixel 1.19, shown at level 0.259, step 1, values float32 |
| 19014 | | |
| 19015 | | > open "/Users/becca/Desktop/Postdoc/COPI notebooks and |
| 19016 | | > resources/Processing/W20_MM_cyto_COPI/W20_J4027_005_volume_map_gammazeta_local.mrc" |
| 19017 | | |
| 19018 | | Opened W20_J4027_005_volume_map_gammazeta_local.mrc as #20, grid size |
| 19019 | | 240,240,240, pixel 1.19, shown at level 0.253, step 1, values float32 |
| 19020 | | |
| 19021 | | > hide #!17 models |
| 19022 | | |
| 19023 | | > hide #!16 models |
| 19024 | | |
| 19025 | | > fitmap #18 inMap #1 |
| 19026 | | |
| 19027 | | Fit map cryosparc_P126_J4027_005_volume_map_half_A.mrc in map |
| 19028 | | W20_J3623_consensus_structure.mrc using 138063 points |
| 19029 | | correlation = 0.9242, correlation about mean = 0.4068, overlap = 1.895e+04 |
| 19030 | | steps = 64, shift = 0.865, angle = 0.418 degrees |
| 19031 | | |
| 19032 | | Position of cryosparc_P126_J4027_005_volume_map_half_A.mrc (#18) relative to |
| 19033 | | W20_J3623_consensus_structure.mrc (#1) coordinates: |
| 19034 | | Matrix rotation and translation |
| 19035 | | 0.99997669 -0.00229559 0.00643030 -1.04477946 |
| 19036 | | 0.00227893 0.99999403 0.00259782 -0.33276084 |
| 19037 | | -0.00643622 -0.00258311 0.99997595 1.91301880 |
| 19038 | | Axis -0.35472898 0.88094699 0.31320880 |
| 19039 | | Axis point 263.82795379 0.00000000 126.10679381 |
| 19040 | | Rotation angle (degrees) 0.41841556 |
| 19041 | | Shift along axis 0.67664322 |
| 19042 | | |
| 19043 | | |
| 19044 | | > fitmap #19 inMap #1 |
| 19045 | | |
| 19046 | | Fit map cryosparc_P126_J4027_005_volume_map_half_B.mrc in map |
| 19047 | | W20_J3623_consensus_structure.mrc using 138195 points |
| 19048 | | correlation = 0.9253, correlation about mean = 0.4118, overlap = 1.9e+04 |
| 19049 | | steps = 68, shift = 0.852, angle = 0.431 degrees |
| 19050 | | |
| 19051 | | Position of cryosparc_P126_J4027_005_volume_map_half_B.mrc (#19) relative to |
| 19052 | | W20_J3623_consensus_structure.mrc (#1) coordinates: |
| 19053 | | Matrix rotation and translation |
| 19054 | | 0.99997498 -0.00277336 0.00650725 -0.98066980 |
| 19055 | | 0.00275654 0.99999284 0.00259285 -0.39125472 |
| 19056 | | -0.00651439 -0.00257484 0.99997547 1.90493875 |
| 19057 | | Axis -0.34310644 0.86456626 0.36715549 |
| 19058 | | Axis point 252.66913251 0.00000000 114.89251767 |
| 19059 | | Rotation angle (degrees) 0.43148350 |
| 19060 | | Shift along axis 0.69761723 |
| 19061 | | |
| 19062 | | |
| 19063 | | > fitmap #20 inMap #1 |
| 19064 | | |
| 19065 | | Fit map W20_J4027_005_volume_map_gammazeta_local.mrc in map |
| 19066 | | W20_J3623_consensus_structure.mrc using 138160 points |
| 19067 | | correlation = 0.9373, correlation about mean = 0.4299, overlap = 1.864e+04 |
| 19068 | | steps = 64, shift = 0.871, angle = 0.411 degrees |
| 19069 | | |
| 19070 | | Position of W20_J4027_005_volume_map_gammazeta_local.mrc (#20) relative to |
| 19071 | | W20_J3623_consensus_structure.mrc (#1) coordinates: |
| 19072 | | Matrix rotation and translation |
| 19073 | | 0.99997680 -0.00234496 0.00639584 -1.04246365 |
| 19074 | | 0.00233059 0.99999474 0.00225441 -0.28336984 |
| 19075 | | -0.00640109 -0.00223945 0.99997701 1.85486702 |
| 19076 | | Axis -0.31324184 0.89200052 0.32590584 |
| 19077 | | Axis point 254.76967129 0.00000000 130.69543046 |
| 19078 | | Rotation angle (degrees) 0.41099531 |
| 19079 | | Shift along axis 0.67828918 |
| 19080 | | |
| 19081 | | |
| 19082 | | > volume resample #19 #20 #18 onGrid #1 |
| 19083 | | |
| 19084 | | Opened cryosparc_P126_J4027_005_volume_map_half_B.mrc resampled as #21, grid |
| 19085 | | size 240,240,240, pixel 1.19, shown at step 1, values float32 |
| 19086 | | Opened W20_J4027_005_volume_map_gammazeta_local.mrc resampled as #22, grid |
| 19087 | | size 240,240,240, pixel 1.19, shown at step 1, values float32 |
| 19088 | | Opened cryosparc_P126_J4027_005_volume_map_half_A.mrc resampled as #23, grid |
| 19089 | | size 240,240,240, pixel 1.19, shown at step 1, values float32 |
| 19090 | | |
| 19091 | | > save W20_J4027_halfB_consensus_resampled.mrc #21 |
| 19092 | | |
| 19093 | | > save W20_J4027_halfA_consensus_resampled.mrc #23 |
| 19094 | | |
| 19095 | | > save W20_J4027_consensus_resampled.mrc #22 |
| 19096 | | |
| 19097 | | > close #18-20 |
| 19098 | | |
| 19099 | | > open "/Users/becca/Desktop/Postdoc/COPI notebooks and |
| 19100 | | > resources/Processing/W20_MM_cyto_COPI/W20_J4027_consensus_resampled.mrc" |
| 19101 | | |
| 19102 | | Opened W20_J4027_consensus_resampled.mrc as #18, grid size 240,240,240, pixel |
| 19103 | | 1.19, shown at level 0.253, step 1, values float32 |
| 19104 | | |
| 19105 | | > open "/Users/becca/Desktop/Postdoc/COPI notebooks and |
| 19106 | | > resources/Processing/W20_MM_cyto_COPI/W20_J4027_halfA_consensus_resampled.mrc" |
| 19107 | | > "/Users/becca/Desktop/Postdoc/COPI notebooks and |
| 19108 | | > resources/Processing/W20_MM_cyto_COPI/W20_J4027_halfB_consensus_resampled.mrc" |
| 19109 | | |
| 19110 | | Opened W20_J4027_halfA_consensus_resampled.mrc as #19.1, grid size |
| 19111 | | 240,240,240, pixel 1.19, shown at level 0.254, step 1, values float32 |
| 19112 | | Opened W20_J4027_halfB_consensus_resampled.mrc as #19.2, grid size |
| 19113 | | 240,240,240, pixel 1.19, shown at level 0.253, step 1, values float32 |
| 19114 | | |
| 19115 | | > rename #19 J4017_half_maps |
| 19116 | | |
| 19117 | | > rename #19 J4027_half_maps |
| 19118 | | |
| 19119 | | > close #21-23 |
| 19120 | | |
| 19121 | | > open "/Users/becca/Desktop/Postdoc/COPI notebooks and |
| 19122 | | > resources/Processing/W20_MM_cyto_COPI/cryosparc_P126_J4028_004_volume_map_half_A.mrc" |
| 19123 | | |
| 19124 | | Opened cryosparc_P126_J4028_004_volume_map_half_A.mrc as #20, grid size |
| 19125 | | 240,240,240, pixel 1.19, shown at level 0.261, step 1, values float32 |
| 19126 | | |
| 19127 | | > open "/Users/becca/Desktop/Postdoc/COPI notebooks and |
| 19128 | | > resources/Processing/W20_MM_cyto_COPI/cryosparc_P126_J4028_004_volume_map_half_B.mrc" |
| 19129 | | |
| 19130 | | Opened cryosparc_P126_J4028_004_volume_map_half_B.mrc as #21, grid size |
| 19131 | | 240,240,240, pixel 1.19, shown at level 0.261, step 1, values float32 |
| 19132 | | |
| 19133 | | > open "/Users/becca/Desktop/Postdoc/COPI notebooks and |
| 19134 | | > resources/Processing/W20_MM_cyto_COPI/W20_J4028_004_volume_map_gammazeta_local.mrc" |
| 19135 | | |
| 19136 | | Opened W20_J4028_004_volume_map_gammazeta_local.mrc as #22, grid size |
| 19137 | | 240,240,240, pixel 1.19, shown at level 0.255, step 1, values float32 |
| 19138 | | |
| 19139 | | > hide #!19 models |
| 19140 | | |
| 19141 | | > hide #!18 models |
| 19142 | | |
| 19143 | | > fitmap #20 inMap #1 |
| 19144 | | |
| 19145 | | Fit map cryosparc_P126_J4028_004_volume_map_half_A.mrc in map |
| 19146 | | W20_J3623_consensus_structure.mrc using 138211 points |
| 19147 | | correlation = 0.9192, correlation about mean = 0.3816, overlap = 1.899e+04 |
| 19148 | | steps = 52, shift = 0.522, angle = 0.296 degrees |
| 19149 | | |
| 19150 | | Position of cryosparc_P126_J4028_004_volume_map_half_A.mrc (#20) relative to |
| 19151 | | W20_J3623_consensus_structure.mrc (#1) coordinates: |
| 19152 | | Matrix rotation and translation |
| 19153 | | 0.99998706 0.00198963 -0.00468116 0.25998221 |
| 19154 | | -0.00198544 0.99999762 0.00090051 0.55733772 |
| 19155 | | 0.00468294 -0.00089120 0.99998864 -0.22070790 |
| 19156 | | Axis -0.17345663 -0.90654298 -0.38482804 |
| 19157 | | Axis point 85.46846238 0.00000000 38.05634596 |
| 19158 | | Rotation angle (degrees) 0.29591860 |
| 19159 | | Shift along axis -0.46541164 |
| 19160 | | |
| 19161 | | |
| 19162 | | > fitmap #21 inMap #1 |
| 19163 | | |
| 19164 | | Fit map cryosparc_P126_J4028_004_volume_map_half_B.mrc in map |
| 19165 | | W20_J3623_consensus_structure.mrc using 138125 points |
| 19166 | | correlation = 0.9186, correlation about mean = 0.3805, overlap = 1.898e+04 |
| 19167 | | steps = 48, shift = 0.516, angle = 0.21 degrees |
| 19168 | | |
| 19169 | | Position of cryosparc_P126_J4028_004_volume_map_half_B.mrc (#21) relative to |
| 19170 | | W20_J3623_consensus_structure.mrc (#1) coordinates: |
| 19171 | | Matrix rotation and translation |
| 19172 | | 0.99999365 0.00064252 -0.00350470 0.30153938 |
| 19173 | | -0.00063949 0.99999942 0.00086460 0.34856397 |
| 19174 | | 0.00350526 -0.00086235 0.99999348 -0.04103359 |
| 19175 | | Axis -0.23552090 -0.95601319 -0.17483906 |
| 19176 | | Axis point 31.62231369 0.00000000 59.29711678 |
| 19177 | | Rotation angle (degrees) 0.21006100 |
| 19178 | | Shift along axis -0.39707630 |
| 19179 | | |
| 19180 | | |
| 19181 | | > fitmap #22 inMap #1 |
| 19182 | | |
| 19183 | | Fit map W20_J4028_004_volume_map_gammazeta_local.mrc in map |
| 19184 | | W20_J3623_consensus_structure.mrc using 138218 points |
| 19185 | | correlation = 0.9302, correlation about mean = 0.3927, overlap = 1.873e+04 |
| 19186 | | steps = 52, shift = 0.535, angle = 0.265 degrees |
| 19187 | | |
| 19188 | | Position of W20_J4028_004_volume_map_gammazeta_local.mrc (#22) relative to |
| 19189 | | W20_J3623_consensus_structure.mrc (#1) coordinates: |
| 19190 | | Matrix rotation and translation |
| 19191 | | 0.99998992 0.00112578 -0.00434648 0.33637900 |
| 19192 | | -0.00112111 0.99999879 0.00107657 0.40418343 |
| 19193 | | 0.00434769 -0.00107168 0.99998997 -0.12603288 |
| 19194 | | Axis -0.23266548 -0.94161994 -0.24334886 |
| 19195 | | Axis point 53.07995958 0.00000000 54.34764883 |
| 19196 | | Rotation angle (degrees) 0.26451275 |
| 19197 | | Shift along axis -0.42818100 |
| 19198 | | |
| 19199 | | |
| 19200 | | > volume resample #21 #20 #22 onGrid #1 |
| 19201 | | |
| 19202 | | Opened cryosparc_P126_J4028_004_volume_map_half_B.mrc resampled as #23, grid |
| 19203 | | size 240,240,240, pixel 1.19, shown at step 1, values float32 |
| 19204 | | Opened cryosparc_P126_J4028_004_volume_map_half_A.mrc resampled as #91, grid |
| 19205 | | size 240,240,240, pixel 1.19, shown at step 1, values float32 |
| 19206 | | Opened W20_J4028_004_volume_map_gammazeta_local.mrc resampled as #92, grid |
| 19207 | | size 240,240,240, pixel 1.19, shown at step 1, values float32 |
| 19208 | | |
| 19209 | | > save W20_J4028_halfA_consensus_resampled.mrc #91 |
| 19210 | | |
| 19211 | | > save W20_J4028_halfB_consensus_resampled.mrc #23 |
| 19212 | | |
| 19213 | | > save W20_J4028_consensus_resampled.mrc #92 |
| 19214 | | |
| 19215 | | > close #20-22 |
| 19216 | | |
| 19217 | | > open "/Users/becca/Desktop/Postdoc/COPI notebooks and |
| 19218 | | > resources/Processing/W20_MM_cyto_COPI/W20_J4028_consensus_resampled.mrc" |
| 19219 | | |
| 19220 | | Opened W20_J4028_consensus_resampled.mrc as #20, grid size 240,240,240, pixel |
| 19221 | | 1.19, shown at level 0.255, step 1, values float32 |
| 19222 | | |
| 19223 | | > open "/Users/becca/Desktop/Postdoc/COPI notebooks and |
| 19224 | | > resources/Processing/W20_MM_cyto_COPI/W20_J4028_halfA_consensus_resampled.mrc" |
| 19225 | | > "/Users/becca/Desktop/Postdoc/COPI notebooks and |
| 19226 | | > resources/Processing/W20_MM_cyto_COPI/W20_J4028_halfB_consensus_resampled.mrc" |
| 19227 | | |
| 19228 | | Opened W20_J4028_halfA_consensus_resampled.mrc as #21.1, grid size |
| 19229 | | 240,240,240, pixel 1.19, shown at level 0.256, step 1, values float32 |
| 19230 | | Opened W20_J4028_halfB_consensus_resampled.mrc as #21.2, grid size |
| 19231 | | 240,240,240, pixel 1.19, shown at level 0.256, step 1, values float32 |
| 19232 | | |
| 19233 | | > rename #21 J4028_half_maps |
| 19234 | | |
| 19235 | | > combine #24 |
| 19236 | | |
| 19237 | | [Repeated 1 time(s)] |
| 19238 | | |
| 19239 | | > close #22-24 |
| 19240 | | |
| 19241 | | > close #25 |
| 19242 | | |
| 19243 | | > close #26 |
| 19244 | | |
| 19245 | | > close #27 |
| 19246 | | |
| 19247 | | > hide #!93 models |
| 19248 | | |
| 19249 | | > hide #!92 models |
| 19250 | | |
| 19251 | | > open "/Users/becca/Desktop/Postdoc/COPI notebooks and |
| 19252 | | > resources/Processing/W20_MM_cyto_COPI/cryosparc_P126_J3988_003_volume_map_half_A.mrc" |
| 19253 | | |
| 19254 | | Opened cryosparc_P126_J3988_003_volume_map_half_A.mrc as #22, grid size |
| 19255 | | 240,240,240, pixel 1.2, shown at level 0.291, step 1, values float32 |
| 19256 | | |
| 19257 | | > open "/Users/becca/Desktop/Postdoc/COPI notebooks and |
| 19258 | | > resources/Processing/W20_MM_cyto_COPI/cryosparc_P126_J3988_003_volume_map_half_B.mrc" |
| 19259 | | |
| 19260 | | Opened cryosparc_P126_J3988_003_volume_map_half_B.mrc as #23, grid size |
| 19261 | | 240,240,240, pixel 1.2, shown at level 0.291, step 1, values float32 |
| 19262 | | |
| 19263 | | > select add #22 |
| 19264 | | |
| 19265 | | 2 models selected |
| 19266 | | |
| 19267 | | > select add #23 |
| 19268 | | |
| 19269 | | 4 models selected |
| 19270 | | |
| 19271 | | > hide #!21 models |
| 19272 | | |
| 19273 | | > hide #!20 models |
| 19274 | | |
| 19275 | | > ui mousemode right "rotate selected models" |
| 19276 | | |
| 19277 | | > view matrix models |
| 19278 | | > #22,-0.85576,-0.11016,-0.5055,355.25,-0.33846,0.85818,0.38596,14.029,0.3913,0.50138,-0.77169,124.47,#23,-0.85576,-0.11016,-0.5055,355.25,-0.33846,0.85818,0.38596,14.029,0.3913,0.50138,-0.77169,124.47 |
| 19279 | | |
| 19280 | | > view matrix models |
| 19281 | | > #22,-0.53192,-0.84561,0.044824,336.27,-0.68771,0.46227,0.55979,96.534,-0.49408,0.26694,-0.82742,293.75,#23,-0.53192,-0.84561,0.044824,336.27,-0.68771,0.46227,0.55979,96.534,-0.49408,0.26694,-0.82742,293.75 |
| 19282 | | |
| 19283 | | > ui mousemode right "translate selected models" |
| 19284 | | |
| 19285 | | > view matrix models |
| 19286 | | > #22,-0.53192,-0.84561,0.044824,335.35,-0.68771,0.46227,0.55979,94.798,-0.49408,0.26694,-0.82742,289.5,#23,-0.53192,-0.84561,0.044824,335.35,-0.68771,0.46227,0.55979,94.798,-0.49408,0.26694,-0.82742,289.5 |
| 19287 | | |
| 19288 | | > view matrix models |
| 19289 | | > #22,-0.53192,-0.84561,0.044824,337.81,-0.68771,0.46227,0.55979,89.918,-0.49408,0.26694,-0.82742,289.61,#23,-0.53192,-0.84561,0.044824,337.81,-0.68771,0.46227,0.55979,89.918,-0.49408,0.26694,-0.82742,289.61 |
| 19290 | | |
| 19291 | | > hide #!91 models |
| 19292 | | |
| 19293 | | > open "/Users/becca/Desktop/Postdoc/COPI notebooks and |
| 19294 | | > resources/Processing/W20_MM_cyto_COPI/W20_J3988_004_volume_map_csparc_refine_of_relion_job126_gammazeta_mask.mrc" |
| 19295 | | |
| 19296 | | Opened |
| 19297 | | W20_J3988_004_volume_map_csparc_refine_of_relion_job126_gammazeta_mask.mrc as |
| 19298 | | #24, grid size 240,240,240, pixel 1.2, shown at level 0.282, step 1, values |
| 19299 | | float32 |
| 19300 | | |
| 19301 | | > select add #24 |
| 19302 | | |
| 19303 | | 6 models selected |
| 19304 | | |
| 19305 | | > select subtract #23 |
| 19306 | | |
| 19307 | | 4 models selected |
| 19308 | | |
| 19309 | | > select subtract #22 |
| 19310 | | |
| 19311 | | 2 models selected |
| 19312 | | |
| 19313 | | > view matrix models #24,1,0,0,4.4068,0,1,0,-1.5627,0,0,1,7.0623 |
| 19314 | | |
| 19315 | | > show #!1 models |
| 19316 | | |
| 19317 | | > ui mousemode right "rotate selected models" |
| 19318 | | |
| 19319 | | > view matrix models |
| 19320 | | > #24,-0.59721,0.29169,0.74717,82.383,0.43729,-0.66251,0.60816,89.121,0.6724,0.68993,0.2681,-83.296 |
| 19321 | | |
| 19322 | | > view matrix models |
| 19323 | | > #24,0.58998,-0.6462,-0.4841,224.91,-0.8066,-0.49879,-0.31719,374.29,-0.036497,0.57761,-0.8155,188.31 |
| 19324 | | |
| 19325 | | > view matrix models |
| 19326 | | > #24,-0.46477,-0.87473,-0.13728,358.04,-0.68335,0.25576,0.68383,105.04,-0.56305,0.41163,-0.71661,272.86 |
| 19327 | | |
| 19328 | | > fitmap #22 inMap #1 |
| 19329 | | |
| 19330 | | Fit map cryosparc_P126_J3988_003_volume_map_half_A.mrc in map |
| 19331 | | W20_J3623_consensus_structure.mrc using 138175 points |
| 19332 | | correlation = 0.9519, correlation about mean = 0.5985, overlap = 2.143e+04 |
| 19333 | | steps = 320, shift = 8.89, angle = 19.2 degrees |
| 19334 | | |
| 19335 | | Position of cryosparc_P126_J3988_003_volume_map_half_A.mrc (#22) relative to |
| 19336 | | W20_J3623_consensus_structure.mrc (#1) coordinates: |
| 19337 | | Matrix rotation and translation |
| 19338 | | -0.33642694 -0.92057260 -0.19840110 353.49844057 |
| 19339 | | -0.77937135 0.15392648 0.60736063 147.09880294 |
| 19340 | | -0.52858037 0.35896062 -0.76925292 278.05341217 |
| 19341 | | Axis -0.56888343 0.75617354 0.32337782 |
| 19342 | | Axis point 252.39557005 0.00000000 81.74420969 |
| 19343 | | Rotation angle (degrees) 167.38948043 |
| 19344 | | Shift along axis 0.04912198 |
| 19345 | | |
| 19346 | | |
| 19347 | | > fitmap #23 inMap #1 |
| 19348 | | |
| 19349 | | Fit map cryosparc_P126_J3988_003_volume_map_half_B.mrc in map |
| 19350 | | W20_J3623_consensus_structure.mrc using 138208 points |
| 19351 | | correlation = 0.9526, correlation about mean = 0.6, overlap = 2.142e+04 |
| 19352 | | steps = 324, shift = 8.99, angle = 19.2 degrees |
| 19353 | | |
| 19354 | | Position of cryosparc_P126_J3988_003_volume_map_half_B.mrc (#23) relative to |
| 19355 | | W20_J3623_consensus_structure.mrc (#1) coordinates: |
| 19356 | | Matrix rotation and translation |
| 19357 | | -0.33657596 -0.92064163 -0.19782722 353.45179614 |
| 19358 | | -0.77865335 0.15396136 0.60827203 146.98371640 |
| 19359 | | -0.52954280 0.35876857 -0.76868038 278.15909277 |
| 19360 | | Axis -0.56874816 0.75615237 0.32366516 |
| 19361 | | Axis point 252.42080897 0.00000000 81.66752530 |
| 19362 | | Rotation angle (degrees) 167.32947007 |
| 19363 | | Shift along axis 0.14743379 |
| 19364 | | |
| 19365 | | |
| 19366 | | > fitmap #24 inMap #1 |
| 19367 | | |
| 19368 | | Fit map |
| 19369 | | W20_J3988_004_volume_map_csparc_refine_of_relion_job126_gammazeta_mask.mrc in |
| 19370 | | map W20_J3623_consensus_structure.mrc using 138204 points |
| 19371 | | correlation = 0.9643, correlation about mean = 0.6577, overlap = 2.107e+04 |
| 19372 | | steps = 104, shift = 5.31, angle = 9.46 degrees |
| 19373 | | |
| 19374 | | Position of |
| 19375 | | W20_J3988_004_volume_map_csparc_refine_of_relion_job126_gammazeta_mask.mrc |
| 19376 | | (#24) relative to W20_J3623_consensus_structure.mrc (#1) coordinates: |
| 19377 | | Matrix rotation and translation |
| 19378 | | -0.33645113 -0.92061038 -0.19818457 353.46943026 |
| 19379 | | -0.77924371 0.15400594 0.60750421 147.10114448 |
| 19380 | | -0.52875310 0.35882956 -0.76919534 278.08170115 |
| 19381 | | Axis -0.56885571 0.75619205 0.32338333 |
| 19382 | | Axis point 252.40251661 0.00000000 81.72856043 |
| 19383 | | Rotation angle (degrees) 167.37468234 |
| 19384 | | Shift along axis 0.09059859 |
| 19385 | | |
| 19386 | | |
| 19387 | | > select subtract #24 |
| 19388 | | |
| 19389 | | Nothing selected |
| 19390 | | |
| 19391 | | > volume resample #24 #23 #22 onGrid #1 |
| 19392 | | |
| 19393 | | Opened |
| 19394 | | W20_J3988_004_volume_map_csparc_refine_of_relion_job126_gammazeta_mask.mrc |
| 19395 | | resampled as #25, grid size 240,240,240, pixel 1.19, shown at step 1, values |
| 19396 | | float32 |
| 19397 | | Opened cryosparc_P126_J3988_003_volume_map_half_B.mrc resampled as #26, grid |
| 19398 | | size 240,240,240, pixel 1.19, shown at step 1, values float32 |
| 19399 | | Opened cryosparc_P126_J3988_003_volume_map_half_A.mrc resampled as #27, grid |
| 19400 | | size 240,240,240, pixel 1.19, shown at step 1, values float32 |
| 19401 | | |
| 19402 | | > save W20_J3988_halfB_consensus_resampled.mrc #26 |
| 19403 | | |
| 19404 | | > save W20_J3988_halfA_consensus_resampled.mrc #27 |
| 19405 | | |
| 19406 | | > save W20_J3988_consensus_resampled.mrc #25 |
| 19407 | | |
| 19408 | | > close #22-24 |
| 19409 | | |
| 19410 | | > hide #!25 models |
| 19411 | | |
| 19412 | | > hide #!26 models |
| 19413 | | |
| 19414 | | > hide #!27 models |
| 19415 | | |
| 19416 | | > open "/Users/becca/Desktop/Postdoc/COPI notebooks and |
| 19417 | | > resources/Processing/W20_MM_cyto_COPI/W20_J3988_consensus_resampled.mrc" |
| 19418 | | |
| 19419 | | Opened W20_J3988_consensus_resampled.mrc as #22, grid size 240,240,240, pixel |
| 19420 | | 1.19, shown at level 0.286, step 1, values float32 |
| 19421 | | |
| 19422 | | > open "/Users/becca/Desktop/Postdoc/COPI notebooks and |
| 19423 | | > resources/Processing/W20_MM_cyto_COPI/W20_J3988_halfA_consensus_resampled.mrc" |
| 19424 | | > "/Users/becca/Desktop/Postdoc/COPI notebooks and |
| 19425 | | > resources/Processing/W20_MM_cyto_COPI/W20_J3988_halfB_consensus_resampled.mrc" |
| 19426 | | |
| 19427 | | Opened W20_J3988_halfA_consensus_resampled.mrc as #23.1, grid size |
| 19428 | | 240,240,240, pixel 1.19, shown at level 0.29, step 1, values float32 |
| 19429 | | Opened W20_J3988_halfB_consensus_resampled.mrc as #23.2, grid size |
| 19430 | | 240,240,240, pixel 1.19, shown at level 0.29, step 1, values float32 |
| 19431 | | |
| 19432 | | > rename #23 J3988_half_maps |
| 19433 | | |
| 19434 | | > close #25-27 |
| 19435 | | |
| 19436 | | > show #!28 models |
| 19437 | | |
| 19438 | | > hide #!28 models |
| 19439 | | |
| 19440 | | > rename #28 COPI_cyto_for_J4027_v1 |
| 19441 | | |
| 19442 | | > hide #!23 models |
| 19443 | | |
| 19444 | | > hide #!22 models |
| 19445 | | |
| 19446 | | > save "/Users/becca/Desktop/Postdoc/COPI notebooks and |
| 19447 | | > resources/Processing/W20_MM_cyto_COPI/20250707_W20_COPI_cyto_alignments.cxs" |
| 19448 | | |
| 19449 | | > open |
| 19450 | | > /Users/becca/Desktop/Postdoc/Phenix/W20_MM_cyto_COPI/LocalResolution_21/W20_J3623_consensus_structure_full.ccp4 |
| 19451 | | |
| 19452 | | Opened W20_J3623_consensus_structure_full.ccp4 as #24, grid size 240,240,240, |
| 19453 | | pixel 1.19, shown at level 0.257, step 1, values float32 |
| 19454 | | |
| 19455 | | > open |
| 19456 | | > /Users/becca/Desktop/Postdoc/Phenix/W20_MM_cyto_COPI/LocalResolution_21/W20_J3623_consensus_structure_local_resolution.ccp4 |
| 19457 | | |
| 19458 | | Opened W20_J3623_consensus_structure_local_resolution.ccp4 as #25, grid size |
| 19459 | | 240,240,240, pixel 1.19, shown at level 14, step 1, values float32 |
| 19460 | | |
| 19461 | | > hide #!25 models |
| 19462 | | |
| 19463 | | > show #!25 models |
| 19464 | | |
| 19465 | | > hide #!25 models |
| 19466 | | |
| 19467 | | > hide #!24 models |
| 19468 | | |
| 19469 | | > show #!24 models |
| 19470 | | |
| 19471 | | > hide #!1 models |
| 19472 | | |
| 19473 | | > color sample #24 map #2 palette |
| 19474 | | > 2.0,purple:2.3,indigo:2.5,blue:2.8,cyan:3.0,lime:3.2, yellow:3.5, |
| 19475 | | > red:4.0,salmon:5.0,pink |
| 19476 | | |
| 19477 | | Invalid "palette" argument: Missing color name or specifier |
| 19478 | | |
| 19479 | | > color sample #24 map #2 |
| 19480 | | |
| 19481 | | Map values for surface "surface": minimum -19.22, mean 2.61, maximum 22.06 |
| 19482 | | |
| 19483 | | > close #24-25 |
| 19484 | | |
| 19485 | | > save "/Users/becca/Desktop/Postdoc/COPI notebooks and |
| 19486 | | > resources/Processing/W20_MM_cyto_COPI/20250707_W20_COPI_cyto_alignments.cxs" |
| 19487 | | |
| 19488 | | > combine #27 |
| 19489 | | |
| 19490 | | No structures specified |
| 19491 | | |
| 19492 | | > combine #29 |
| 19493 | | |
| 19494 | | No structures specified |
| 19495 | | |
| 19496 | | > combine #28 |
| 19497 | | |
| 19498 | | > rename #24 COPI_cyto_for_J4027_v1 |
| 19499 | | |
| 19500 | | > split #24 chains |
| 19501 | | |
| 19502 | | Split COPI_cyto_for_J4027_v1 (#24) into 13 models |
| 19503 | | Chain information for COPI_cyto_for_J4027_v1 A #24.1 |
| 19504 | | --- |
| 19505 | | Chain | Description |
| 19506 | | A | No description available |
| 19507 | | |
| 19508 | | Chain information for COPI_cyto_for_J4027_v1 B #24.2 |
| 19509 | | --- |
| 19510 | | Chain | Description |
| 19511 | | B | No description available |
| 19512 | | |
| 19513 | | Chain information for COPI_cyto_for_J4027_v1 C #24.3 |
| 19514 | | --- |
| 19515 | | Chain | Description |
| 19516 | | C | No description available |
| 19517 | | |
| 19518 | | Chain information for COPI_cyto_for_J4027_v1 D #24.4 |
| 19519 | | --- |
| 19520 | | Chain | Description |
| 19521 | | D | No description available |
| 19522 | | |
| 19523 | | Chain information for COPI_cyto_for_J4027_v1 E #24.5 |
| 19524 | | --- |
| 19525 | | Chain | Description |
| 19526 | | E | No description available |
| 19527 | | |
| 19528 | | Chain information for COPI_cyto_for_J4027_v1 F #24.6 |
| 19529 | | --- |
| 19530 | | Chain | Description |
| 19531 | | F | No description available |
| 19532 | | |
| 19533 | | Chain information for COPI_cyto_for_J4027_v1 G #24.7 |
| 19534 | | --- |
| 19535 | | Chain | Description |
| 19536 | | G | No description available |
| 19537 | | |
| 19538 | | Chain information for COPI_cyto_for_J4027_v1 H #24.8 |
| 19539 | | --- |
| 19540 | | Chain | Description |
| 19541 | | H | No description available |
| 19542 | | |
| 19543 | | Chain information for COPI_cyto_for_J4027_v1 I #24.9 |
| 19544 | | --- |
| 19545 | | Chain | Description |
| 19546 | | I | No description available |
| 19547 | | |
| 19548 | | Chain information for COPI_cyto_for_J4027_v1 J #24.10 |
| 19549 | | --- |
| 19550 | | Chain | Description |
| 19551 | | J | No description available |
| 19552 | | |
| 19553 | | Chain information for COPI_cyto_for_J4027_v1 K #24.11 |
| 19554 | | --- |
| 19555 | | Chain | Description |
| 19556 | | K | No description available |
| 19557 | | |
| 19558 | | Chain information for COPI_cyto_for_J4027_v1 L #24.12 |
| 19559 | | --- |
| 19560 | | Chain | Description |
| 19561 | | L | No description available |
| 19562 | | |
| 19563 | | Chain information for COPI_cyto_for_J4027_v1 M #24.13 |
| 19564 | | --- |
| 19565 | | Chain | Description |
| 19566 | | M | No description available |
| 19567 | | |
| 19568 | | |
| 19569 | | > show #!1 models |
| 19570 | | |
| 19571 | | > color #1 darkgrey models |
| 19572 | | |
| 19573 | | > color #1 #a9a9a9c0 models |
| 19574 | | |
| 19575 | | > rename #24 COPI_cyto_J3623_model |
| 19576 | | |
| 19577 | | > hide #!24 models |
| 19578 | | |
| 19579 | | > show #!24 models |
| 19580 | | |
| 19581 | | > hide #!1 models |
| 19582 | | |
| 19583 | | > hide #!24 models |
| 19584 | | |
| 19585 | | > combine #28 |
| 19586 | | |
| 19587 | | > split #25 chains |
| 19588 | | |
| 19589 | | Split copy of COPI_cyto_for_J4027_v1 (#25) into 13 models |
| 19590 | | Chain information for copy of COPI_cyto_for_J4027_v1 A #25.1 |
| 19591 | | --- |
| 19592 | | Chain | Description |
| 19593 | | A | No description available |
| 19594 | | |
| 19595 | | Chain information for copy of COPI_cyto_for_J4027_v1 B #25.2 |
| 19596 | | --- |
| 19597 | | Chain | Description |
| 19598 | | B | No description available |
| 19599 | | |
| 19600 | | Chain information for copy of COPI_cyto_for_J4027_v1 C #25.3 |
| 19601 | | --- |
| 19602 | | Chain | Description |
| 19603 | | C | No description available |
| 19604 | | |
| 19605 | | Chain information for copy of COPI_cyto_for_J4027_v1 D #25.4 |
| 19606 | | --- |
| 19607 | | Chain | Description |
| 19608 | | D | No description available |
| 19609 | | |
| 19610 | | Chain information for copy of COPI_cyto_for_J4027_v1 E #25.5 |
| 19611 | | --- |
| 19612 | | Chain | Description |
| 19613 | | E | No description available |
| 19614 | | |
| 19615 | | Chain information for copy of COPI_cyto_for_J4027_v1 F #25.6 |
| 19616 | | --- |
| 19617 | | Chain | Description |
| 19618 | | F | No description available |
| 19619 | | |
| 19620 | | Chain information for copy of COPI_cyto_for_J4027_v1 G #25.7 |
| 19621 | | --- |
| 19622 | | Chain | Description |
| 19623 | | G | No description available |
| 19624 | | |
| 19625 | | Chain information for copy of COPI_cyto_for_J4027_v1 H #25.8 |
| 19626 | | --- |
| 19627 | | Chain | Description |
| 19628 | | H | No description available |
| 19629 | | |
| 19630 | | Chain information for copy of COPI_cyto_for_J4027_v1 I #25.9 |
| 19631 | | --- |
| 19632 | | Chain | Description |
| 19633 | | I | No description available |
| 19634 | | |
| 19635 | | Chain information for copy of COPI_cyto_for_J4027_v1 J #25.10 |
| 19636 | | --- |
| 19637 | | Chain | Description |
| 19638 | | J | No description available |
| 19639 | | |
| 19640 | | Chain information for copy of COPI_cyto_for_J4027_v1 K #25.11 |
| 19641 | | --- |
| 19642 | | Chain | Description |
| 19643 | | K | No description available |
| 19644 | | |
| 19645 | | Chain information for copy of COPI_cyto_for_J4027_v1 L #25.12 |
| 19646 | | --- |
| 19647 | | Chain | Description |
| 19648 | | L | No description available |
| 19649 | | |
| 19650 | | Chain information for copy of COPI_cyto_for_J4027_v1 M #25.13 |
| 19651 | | --- |
| 19652 | | Chain | Description |
| 19653 | | M | No description available |
| 19654 | | |
| 19655 | | |
| 19656 | | > show #!4 models |
| 19657 | | |
| 19658 | | > rename #25 COPI_cyto_for_J4027_v1 |
| 19659 | | |
| 19660 | | > rename #25 COPI_cyto_J3987_model |
| 19661 | | |
| 19662 | | > fitmap #25.1 inMap #4 |
| 19663 | | |
| 19664 | | Fit molecule copy of COPI_cyto_for_J4027_v1 A (#25.1) to map |
| 19665 | | W20_J3987_consensus_resampled.mrc (#4) using 2590 atoms |
| 19666 | | average map value = 0.3221, steps = 104 |
| 19667 | | shifted from previous position = 0.721 |
| 19668 | | rotated from previous position = 1.24 degrees |
| 19669 | | atoms outside contour = 795, contour level = 0.27665 |
| 19670 | | |
| 19671 | | Position of copy of COPI_cyto_for_J4027_v1 A (#25.1) relative to |
| 19672 | | W20_J3987_consensus_resampled.mrc (#4) coordinates: |
| 19673 | | Matrix rotation and translation |
| 19674 | | -0.38689637 -0.90569538 -0.17328327 278.18188435 |
| 19675 | | -0.79467842 0.23216071 0.56088111 163.52034472 |
| 19676 | | -0.46775786 0.35470734 -0.80955871 239.74100561 |
| 19677 | | Axis -0.54800188 0.78270203 0.29507876 |
| 19678 | | Axis point 210.04111237 0.00000000 70.64408205 |
| 19679 | | Rotation angle (degrees) 169.15725379 |
| 19680 | | Shift along axis 46.28598736 |
| 19681 | | |
| 19682 | | |
| 19683 | | > fitmap #25.2 inMap #4 |
| 19684 | | |
| 19685 | | Fit molecule copy of COPI_cyto_for_J4027_v1 B (#25.2) to map |
| 19686 | | W20_J3987_consensus_resampled.mrc (#4) using 2426 atoms |
| 19687 | | average map value = 0.3153, steps = 72 |
| 19688 | | shifted from previous position = 0.757 |
| 19689 | | rotated from previous position = 2.26 degrees |
| 19690 | | atoms outside contour = 839, contour level = 0.27665 |
| 19691 | | |
| 19692 | | Position of copy of COPI_cyto_for_J4027_v1 B (#25.2) relative to |
| 19693 | | W20_J3987_consensus_resampled.mrc (#4) coordinates: |
| 19694 | | Matrix rotation and translation |
| 19695 | | -0.39471401 -0.89888974 -0.19025799 280.36384015 |
| 19696 | | -0.79142130 0.22743083 0.56738660 162.69216971 |
| 19697 | | -0.46674746 0.37452966 -0.80117062 238.28935068 |
| 19698 | | Axis -0.54506838 0.78143761 0.30373627 |
| 19699 | | Axis point 209.70723896 0.00000000 70.08028018 |
| 19700 | | Rotation angle (degrees) 169.81012722 |
| 19701 | | Shift along axis 46.69343412 |
| 19702 | | |
| 19703 | | |
| 19704 | | > fitmap #25.3 inMap #4 |
| 19705 | | |
| 19706 | | Fit molecule copy of COPI_cyto_for_J4027_v1 C (#25.3) to map |
| 19707 | | W20_J3987_consensus_resampled.mrc (#4) using 1612 atoms |
| 19708 | | average map value = 0.3176, steps = 56 |
| 19709 | | shifted from previous position = 0.728 |
| 19710 | | rotated from previous position = 2.04 degrees |
| 19711 | | atoms outside contour = 641, contour level = 0.27665 |
| 19712 | | |
| 19713 | | Position of copy of COPI_cyto_for_J4027_v1 C (#25.3) relative to |
| 19714 | | W20_J3987_consensus_resampled.mrc (#4) coordinates: |
| 19715 | | Matrix rotation and translation |
| 19716 | | -0.39605343 -0.89988782 -0.18260232 278.68660008 |
| 19717 | | -0.79444663 0.23610267 0.55956242 163.22266002 |
| 19718 | | -0.46043050 0.36668441 -0.80842211 238.93411170 |
| 19719 | | Axis -0.54443563 0.78422400 0.29762824 |
| 19720 | | Axis point 208.79769571 0.00000000 71.16300785 |
| 19721 | | Rotation angle (degrees) 169.79703161 |
| 19722 | | Shift along axis 47.38975067 |
| 19723 | | |
| 19724 | | |
| 19725 | | > fitmap #25.4 inMap #4 |
| 19726 | | |
| 19727 | | Fit molecule copy of COPI_cyto_for_J4027_v1 D (#25.4) to map |
| 19728 | | W20_J3987_consensus_resampled.mrc (#4) using 4745 atoms |
| 19729 | | average map value = 0.3393, steps = 64 |
| 19730 | | shifted from previous position = 0.197 |
| 19731 | | rotated from previous position = 0.999 degrees |
| 19732 | | atoms outside contour = 1721, contour level = 0.27665 |
| 19733 | | |
| 19734 | | Position of copy of COPI_cyto_for_J4027_v1 D (#25.4) relative to |
| 19735 | | W20_J3987_consensus_resampled.mrc (#4) coordinates: |
| 19736 | | Matrix rotation and translation |
| 19737 | | -0.36755215 -0.91697806 -0.15510211 274.92468941 |
| 19738 | | -0.80291601 0.22872446 0.55046437 165.41983596 |
| 19739 | | -0.46928810 0.32685833 -0.82032452 243.67714165 |
| 19740 | | Axis -0.55602986 0.78127043 0.28363236 |
| 19741 | | Axis point 211.32871948 0.00000000 72.29578138 |
| 19742 | | Rotation angle (degrees) 168.40024254 |
| 19743 | | Shift along axis 45.48601304 |
| 19744 | | |
| 19745 | | |
| 19746 | | > fitmap #25.5 inMap #4 |
| 19747 | | |
| 19748 | | Fit molecule copy of COPI_cyto_for_J4027_v1 E (#25.5) to map |
| 19749 | | W20_J3987_consensus_resampled.mrc (#4) using 1876 atoms |
| 19750 | | average map value = 0.3141, steps = 48 |
| 19751 | | shifted from previous position = 0.53 |
| 19752 | | rotated from previous position = 0.888 degrees |
| 19753 | | atoms outside contour = 674, contour level = 0.27665 |
| 19754 | | |
| 19755 | | Position of copy of COPI_cyto_for_J4027_v1 E (#25.5) relative to |
| 19756 | | W20_J3987_consensus_resampled.mrc (#4) coordinates: |
| 19757 | | Matrix rotation and translation |
| 19758 | | -0.38334136 -0.90913799 -0.16284208 277.17803202 |
| 19759 | | -0.79171318 0.23265338 0.56485632 162.82482750 |
| 19760 | | -0.47564657 0.34545700 -0.80896218 242.10131222 |
| 19761 | | Axis -0.54889072 0.78257074 0.29377205 |
| 19762 | | Axis point 210.42677765 0.00000000 70.96904172 |
| 19763 | | Rotation angle (degrees) 168.47140268 |
| 19764 | | Shift along axis 46.40409379 |
| 19765 | | |
| 19766 | | |
| 19767 | | > fitmap #25.6 inMap #4 |
| 19768 | | |
| 19769 | | Fit molecule copy of COPI_cyto_for_J4027_v1 F (#25.6) to map |
| 19770 | | W20_J3987_consensus_resampled.mrc (#4) using 2400 atoms |
| 19771 | | average map value = 0.2626, steps = 84 |
| 19772 | | shifted from previous position = 2.71 |
| 19773 | | rotated from previous position = 2.99 degrees |
| 19774 | | atoms outside contour = 1310, contour level = 0.27665 |
| 19775 | | |
| 19776 | | Position of copy of COPI_cyto_for_J4027_v1 F (#25.6) relative to |
| 19777 | | W20_J3987_consensus_resampled.mrc (#4) coordinates: |
| 19778 | | Matrix rotation and translation |
| 19779 | | -0.40199255 -0.90304968 -0.15133829 274.52161881 |
| 19780 | | -0.76596507 0.24109454 0.59596221 157.65702153 |
| 19781 | | -0.50169664 0.35549220 -0.78862272 240.80158217 |
| 19782 | | Axis -0.53855753 0.78466390 0.30701522 |
| 19783 | | Axis point 207.61107533 0.00000000 67.84938526 |
| 19784 | | Rotation angle (degrees) 167.09978512 |
| 19785 | | Shift along axis 49.79183984 |
| 19786 | | |
| 19787 | | |
| 19788 | | > fitmap #25.7 inMap #4 |
| 19789 | | |
| 19790 | | Fit molecule copy of COPI_cyto_for_J4027_v1 G (#25.7) to map |
| 19791 | | W20_J3987_consensus_resampled.mrc (#4) using 2302 atoms |
| 19792 | | average map value = 0.2759, steps = 60 |
| 19793 | | shifted from previous position = 1.78 |
| 19794 | | rotated from previous position = 1.99 degrees |
| 19795 | | atoms outside contour = 1124, contour level = 0.27665 |
| 19796 | | |
| 19797 | | Position of copy of COPI_cyto_for_J4027_v1 G (#25.7) relative to |
| 19798 | | W20_J3987_consensus_resampled.mrc (#4) coordinates: |
| 19799 | | Matrix rotation and translation |
| 19800 | | -0.37472415 -0.90717861 -0.19133425 277.62448174 |
| 19801 | | -0.79104565 0.20520230 0.57631485 166.08473990 |
| 19802 | | -0.48355827 0.36731321 -0.79451395 238.83413932 |
| 19803 | | Axis -0.55353568 0.77394812 0.30757528 |
| 19804 | | Axis point 211.98709934 0.00000000 67.68536340 |
| 19805 | | Rotation angle (degrees) 169.11794633 |
| 19806 | | Shift along axis 48.32539250 |
| 19807 | | |
| 19808 | | |
| 19809 | | > fitmap #25.8 inMap #4 |
| 19810 | | |
| 19811 | | Fit molecule copy of COPI_cyto_for_J4027_v1 H (#25.8) to map |
| 19812 | | W20_J3987_consensus_resampled.mrc (#4) using 420 atoms |
| 19813 | | average map value = 0.339, steps = 56 |
| 19814 | | shifted from previous position = 1.73 |
| 19815 | | rotated from previous position = 4.37 degrees |
| 19816 | | atoms outside contour = 139, contour level = 0.27665 |
| 19817 | | |
| 19818 | | Position of copy of COPI_cyto_for_J4027_v1 H (#25.8) relative to |
| 19819 | | W20_J3987_consensus_resampled.mrc (#4) coordinates: |
| 19820 | | Matrix rotation and translation |
| 19821 | | -0.30262332 -0.93808653 -0.16856100 274.79297984 |
| 19822 | | -0.83162161 0.17348487 0.52754953 176.28282750 |
| 19823 | | -0.46564432 0.29982776 -0.83263359 248.60795718 |
| 19824 | | Axis -0.58515271 0.76338380 0.27357170 |
| 19825 | | Axis point 220.21870033 0.00000000 73.46391590 |
| 19826 | | Rotation angle (degrees) 168.77962388 |
| 19827 | | Shift along axis 41.78769794 |
| 19828 | | |
| 19829 | | |
| 19830 | | > fitmap #25.9 inMap #4 |
| 19831 | | |
| 19832 | | Fit molecule copy of COPI_cyto_for_J4027_v1 I (#25.9) to map |
| 19833 | | W20_J3987_consensus_resampled.mrc (#4) using 1573 atoms |
| 19834 | | average map value = 0.3297, steps = 60 |
| 19835 | | shifted from previous position = 0.384 |
| 19836 | | rotated from previous position = 0.597 degrees |
| 19837 | | atoms outside contour = 540, contour level = 0.27665 |
| 19838 | | |
| 19839 | | Position of copy of COPI_cyto_for_J4027_v1 I (#25.9) relative to |
| 19840 | | W20_J3987_consensus_resampled.mrc (#4) coordinates: |
| 19841 | | Matrix rotation and translation |
| 19842 | | -0.37451788 -0.91088738 -0.17326443 276.29637371 |
| 19843 | | -0.79727848 0.22096017 0.56171491 164.46884657 |
| 19844 | | -0.47337448 0.34851227 -0.80898443 241.62397254 |
| 19845 | | Axis -0.55339354 0.77897237 0.29488579 |
| 19846 | | Axis point 210.79260550 0.00000000 70.83395311 |
| 19847 | | Rotation angle (degrees) 168.89357390 |
| 19848 | | Shift along axis 46.46753453 |
| 19849 | | |
| 19850 | | |
| 19851 | | > fitmap #25.10 inMap #4 |
| 19852 | | |
| 19853 | | Fit molecule copy of COPI_cyto_for_J4027_v1 J (#25.10) to map |
| 19854 | | W20_J3987_consensus_resampled.mrc (#4) using 1112 atoms |
| 19855 | | average map value = 0.3174, steps = 56 |
| 19856 | | shifted from previous position = 0.808 |
| 19857 | | rotated from previous position = 4.24 degrees |
| 19858 | | atoms outside contour = 445, contour level = 0.27665 |
| 19859 | | |
| 19860 | | Position of copy of COPI_cyto_for_J4027_v1 J (#25.10) relative to |
| 19861 | | W20_J3987_consensus_resampled.mrc (#4) coordinates: |
| 19862 | | Matrix rotation and translation |
| 19863 | | -0.37184146 -0.92295368 -0.09945074 269.60235343 |
| 19864 | | -0.79986239 0.26418072 0.53891439 164.53018199 |
| 19865 | | -0.47112005 0.27993762 -0.83646927 249.12759996 |
| 19866 | | Axis -0.55169100 0.79175677 0.26221794 |
| 19867 | | Axis point 209.92414105 0.00000000 75.11235729 |
| 19868 | | Rotation angle (degrees) 166.42536541 |
| 19869 | | Shift along axis 46.85641909 |
| 19870 | | |
| 19871 | | |
| 19872 | | > fitmap #25.11 inMap #4 |
| 19873 | | |
| 19874 | | Fit molecule copy of COPI_cyto_for_J4027_v1 K (#25.11) to map |
| 19875 | | W20_J3987_consensus_resampled.mrc (#4) using 319 atoms |
| 19876 | | average map value = 0.3247, steps = 356 |
| 19877 | | shifted from previous position = 18 |
| 19878 | | rotated from previous position = 29.8 degrees |
| 19879 | | atoms outside contour = 122, contour level = 0.27665 |
| 19880 | | |
| 19881 | | Position of copy of COPI_cyto_for_J4027_v1 K (#25.11) relative to |
| 19882 | | W20_J3987_consensus_resampled.mrc (#4) coordinates: |
| 19883 | | Matrix rotation and translation |
| 19884 | | -0.11497818 -0.99056474 0.07457564 250.16493380 |
| 19885 | | -0.55287660 0.12618381 0.82365352 113.16758699 |
| 19886 | | -0.82529237 0.05347106 -0.56216845 259.79352847 |
| 19887 | | Axis -0.60992918 0.71263081 0.34661756 |
| 19888 | | Axis point 236.76411941 0.00000000 37.20193482 |
| 19889 | | Rotation angle (degrees) 140.84869902 |
| 19890 | | Shift along axis 18.11281583 |
| 19891 | | |
| 19892 | | |
| 19893 | | > fitmap #25.12 inMap #4 |
| 19894 | | |
| 19895 | | Fit molecule copy of COPI_cyto_for_J4027_v1 L (#25.12) to map |
| 19896 | | W20_J3987_consensus_resampled.mrc (#4) using 1420 atoms |
| 19897 | | average map value = 0.2216, steps = 244 |
| 19898 | | shifted from previous position = 20 |
| 19899 | | rotated from previous position = 43.1 degrees |
| 19900 | | atoms outside contour = 1209, contour level = 0.27665 |
| 19901 | | |
| 19902 | | Position of copy of COPI_cyto_for_J4027_v1 L (#25.12) relative to |
| 19903 | | W20_J3987_consensus_resampled.mrc (#4) coordinates: |
| 19904 | | Matrix rotation and translation |
| 19905 | | -0.33704271 -0.93889900 0.06979181 244.25606438 |
| 19906 | | -0.21172527 0.14781713 0.96608619 12.69558044 |
| 19907 | | -0.91737377 0.31083561 -0.24860932 233.11919004 |
| 19908 | | Axis -0.47133855 0.71009352 0.52307472 |
| 19909 | | Axis point 190.36197930 0.00000000 40.21086330 |
| 19910 | | Rotation angle (degrees) 135.96517505 |
| 19911 | | Shift along axis 15.82650607 |
| 19912 | | |
| 19913 | | |
| 19914 | | > close #25 |
| 19915 | | |
| 19916 | | > combine #28 |
| 19917 | | |
| 19918 | | > color #4 darkgrey models |
| 19919 | | |
| 19920 | | > color #4 #a9a9a9bf models |
| 19921 | | |
| 19922 | | > rename #25 COPI_cyto_J3987_model |
| 19923 | | |
| 19924 | | > fitmap #25 inMap #4 |
| 19925 | | |
| 19926 | | Fit molecule COPI_cyto_J3987_model (#25) to map |
| 19927 | | W20_J3987_consensus_resampled.mrc (#4) using 27347 atoms |
| 19928 | | average map value = 0.2864, steps = 40 |
| 19929 | | shifted from previous position = 0.228 |
| 19930 | | rotated from previous position = 0.219 degrees |
| 19931 | | atoms outside contour = 13455, contour level = 0.27665 |
| 19932 | | |
| 19933 | | Position of COPI_cyto_J3987_model (#25) relative to |
| 19934 | | W20_J3987_consensus_resampled.mrc (#4) coordinates: |
| 19935 | | Matrix rotation and translation |
| 19936 | | -0.37179442 -0.91394156 -0.16272598 275.88087564 |
| 19937 | | -0.79687792 0.22429417 0.56096143 164.32455600 |
| 19938 | | -0.47618747 0.33823507 -0.81169116 242.44716299 |
| 19939 | | Axis -0.55410562 0.77983932 0.29123460 |
| 19940 | | Axis point 211.23166983 0.00000000 70.92651391 |
| 19941 | | Rotation angle (degrees) 168.40582967 |
| 19942 | | Shift along axis 45.88860967 |
| 19943 | | |
| 19944 | | |
| 19945 | | > hide #!25 models |
| 19946 | | |
| 19947 | | > show #!24 models |
| 19948 | | |
| 19949 | | > combine #28 |
| 19950 | | |
| 19951 | | > hide #!4 models |
| 19952 | | |
| 19953 | | > show #!6 models |
| 19954 | | |
| 19955 | | > color #6 darkgrey models |
| 19956 | | |
| 19957 | | > color #6 #a9a9a9bf models |
| 19958 | | |
| 19959 | | > fitmap #26 inMap #6 |
| 19960 | | |
| 19961 | | Fit molecule copy of COPI_cyto_for_J4027_v1 (#26) to map |
| 19962 | | W20_J4021_consensus_resampled.mrc (#6) using 27347 atoms |
| 19963 | | average map value = 0.2574, steps = 88 |
| 19964 | | shifted from previous position = 0.804 |
| 19965 | | rotated from previous position = 1.33 degrees |
| 19966 | | atoms outside contour = 13999, contour level = 0.25941 |
| 19967 | | |
| 19968 | | Position of copy of COPI_cyto_for_J4027_v1 (#26) relative to |
| 19969 | | W20_J4021_consensus_resampled.mrc (#6) coordinates: |
| 19970 | | Matrix rotation and translation |
| 19971 | | -0.39044300 -0.90597356 -0.16360374 277.32294036 |
| 19972 | | -0.78887242 0.23762979 0.56675603 162.18357201 |
| 19973 | | -0.47458885 0.35034840 -0.80747844 241.34338581 |
| 19974 | | Axis -0.54571767 0.78421483 0.29529532 |
| 19975 | | Axis point 209.61127190 0.00000000 70.79010036 |
| 19976 | | Rotation angle (degrees) 168.56372015 |
| 19977 | | Shift along axis 47.11430470 |
| 19978 | | |
| 19979 | | |
| 19980 | | > rename #26 COPI_cyto_J4021_model |
| 19981 | | |
| 19982 | | > hide #!26 models |
| 19983 | | |
| 19984 | | > combine #28 |
| 19985 | | |
| 19986 | | > close #29-33 |
| 19987 | | |
| 19988 | | > rename #27 COPI_cyto_J4022_model |
| 19989 | | |
| 19990 | | > rename #28 COPI_cyto_J4023_model |
| 19991 | | |
| 19992 | | > combine #28 |
| 19993 | | |
| 19994 | | > hide #!27 models |
| 19995 | | |
| 19996 | | > hide #!24 models |
| 19997 | | |
| 19998 | | > rename #29 COPI_cyto_J4024_model |
| 19999 | | |
| 20000 | | > combine #29 |
| 20001 | | |
| 20002 | | [Repeated 1 time(s)] |
| 20003 | | |
| 20004 | | > rename #30 COPI_cyto_J4025_model |
| 20005 | | |
| 20006 | | > rename #31 COPI_cyto_J4026_model |
| 20007 | | |
| 20008 | | > combine #29 |
| 20009 | | |
| 20010 | | > rename #32 COPI_cyto_J4027_model |
| 20011 | | |
| 20012 | | > combine #29 |
| 20013 | | |
| 20014 | | > rename #33 COPI_cyto_J4028_model |
| 20015 | | |
| 20016 | | > hide #!29 models |
| 20017 | | |
| 20018 | | > hide #!30 models |
| 20019 | | |
| 20020 | | > hide #!31 models |
| 20021 | | |
| 20022 | | > hide #!32 models |
| 20023 | | |
| 20024 | | > hide #!33 models |
| 20025 | | |
| 20026 | | > combine #29 |
| 20027 | | |
| 20028 | | > close #35-36,39#34,37-38 |
| 20029 | | |
| 20030 | | > combine #29 |
| 20031 | | |
| 20032 | | > rename #34 COPI_cyto_J3988_model |
| 20033 | | |
| 20034 | | > hide #!34 models |
| 20035 | | |
| 20036 | | > close #40 |
| 20037 | | |
| 20038 | | > close #41 |
| 20039 | | |
| 20040 | | > close #42-46 |
| 20041 | | |
| 20042 | | > hide #!6 models |
| 20043 | | |
| 20044 | | > show #!6 models |
| 20045 | | |
| 20046 | | > hide #!6 models |
| 20047 | | |
| 20048 | | > show #!1 models |
| 20049 | | |
| 20050 | | > fitmap #24.1 inMap #1 |
| 20051 | | |
| 20052 | | Fit molecule COPI_cyto_for_J4027_v1 A (#24.1) to map |
| 20053 | | W20_J3623_consensus_structure.mrc (#1) using 2590 atoms |
| 20054 | | average map value = 0.3549, steps = 116 |
| 20055 | | shifted from previous position = 0.529 |
| 20056 | | rotated from previous position = 1.66 degrees |
| 20057 | | atoms outside contour = 606, contour level = 0.25746 |
| 20058 | | |
| 20059 | | Position of COPI_cyto_for_J4027_v1 A (#24.1) relative to |
| 20060 | | W20_J3623_consensus_structure.mrc (#1) coordinates: |
| 20061 | | Matrix rotation and translation |
| 20062 | | -0.39542896 -0.90304867 -0.16774700 278.17983361 |
| 20063 | | -0.78821131 0.23986960 0.56673233 161.55778287 |
| 20064 | | -0.47154946 0.35632245 -0.80664455 240.52362588 |
| 20065 | | Axis -0.54371860 0.78505369 0.29675038 |
| 20066 | | Axis point 209.13140963 0.00000000 70.83505847 |
| 20067 | | Rotation angle (degrees) 168.84338449 |
| 20068 | | Shift along axis 46.95546224 |
| 20069 | | |
| 20070 | | |
| 20071 | | > fitmap #24.2 inMap #1 |
| 20072 | | |
| 20073 | | Fit molecule COPI_cyto_for_J4027_v1 B (#24.2) to map |
| 20074 | | W20_J3623_consensus_structure.mrc (#1) using 2426 atoms |
| 20075 | | average map value = 0.3443, steps = 84 |
| 20076 | | shifted from previous position = 1.05 |
| 20077 | | rotated from previous position = 2.23 degrees |
| 20078 | | atoms outside contour = 613, contour level = 0.25746 |
| 20079 | | |
| 20080 | | Position of COPI_cyto_for_J4027_v1 B (#24.2) relative to |
| 20081 | | W20_J3623_consensus_structure.mrc (#1) coordinates: |
| 20082 | | Matrix rotation and translation |
| 20083 | | -0.39499091 -0.89904452 -0.18894748 279.87559115 |
| 20084 | | -0.79097752 0.22820260 0.56769548 163.08646468 |
| 20085 | | -0.46726520 0.37368776 -0.80126194 238.60471247 |
| 20086 | | Axis -0.54487475 0.78166114 0.30350844 |
| 20087 | | Axis point 209.64905720 0.00000000 70.09351394 |
| 20088 | | Rotation angle (degrees) 169.74498652 |
| 20089 | | Shift along axis 47.39975428 |
| 20090 | | |
| 20091 | | |
| 20092 | | > fitmap #24.3 inMap #1 |
| 20093 | | |
| 20094 | | Fit molecule COPI_cyto_for_J4027_v1 C (#24.3) to map |
| 20095 | | W20_J3623_consensus_structure.mrc (#1) using 1612 atoms |
| 20096 | | average map value = 0.3398, steps = 68 |
| 20097 | | shifted from previous position = 0.582 |
| 20098 | | rotated from previous position = 1.57 degrees |
| 20099 | | atoms outside contour = 545, contour level = 0.25746 |
| 20100 | | |
| 20101 | | Position of COPI_cyto_for_J4027_v1 C (#24.3) relative to |
| 20102 | | W20_J3623_consensus_structure.mrc (#1) coordinates: |
| 20103 | | Matrix rotation and translation |
| 20104 | | -0.38289473 -0.90536973 -0.18356823 277.48749747 |
| 20105 | | -0.80205073 0.22720768 0.55235071 164.98384273 |
| 20106 | | -0.45837350 0.35872321 -0.81314907 239.04481293 |
| 20107 | | Axis -0.55056634 0.78138966 0.29378038 |
| 20108 | | Axis point 209.64934014 0.00000000 71.32622789 |
| 20109 | | Rotation angle (degrees) 169.87222630 |
| 20110 | | Shift along axis 46.36806823 |
| 20111 | | |
| 20112 | | |
| 20113 | | > fitmap #24.4 inMap #1 |
| 20114 | | |
| 20115 | | Fit molecule COPI_cyto_for_J4027_v1 D (#24.4) to map |
| 20116 | | W20_J3623_consensus_structure.mrc (#1) using 4745 atoms |
| 20117 | | average map value = 0.3686, steps = 104 |
| 20118 | | shifted from previous position = 0.253 |
| 20119 | | rotated from previous position = 1.26 degrees |
| 20120 | | atoms outside contour = 1389, contour level = 0.25746 |
| 20121 | | |
| 20122 | | Position of COPI_cyto_for_J4027_v1 D (#24.4) relative to |
| 20123 | | W20_J3623_consensus_structure.mrc (#1) coordinates: |
| 20124 | | Matrix rotation and translation |
| 20125 | | -0.36437524 -0.91895662 -0.15082912 274.51036554 |
| 20126 | | -0.80384056 0.22859535 0.54916713 165.68273324 |
| 20127 | | -0.47018193 0.32134547 -0.82198909 244.27756191 |
| 20128 | | Axis -0.55725502 0.78114152 0.28157552 |
| 20129 | | Axis point 211.66245571 0.00000000 72.46008698 |
| 20130 | | Rotation angle (degrees) 168.20479189 |
| 20131 | | Shift along axis 45.23196284 |
| 20132 | | |
| 20133 | | |
| 20134 | | > fitmap #24.5 inMap #1 |
| 20135 | | |
| 20136 | | Fit molecule COPI_cyto_for_J4027_v1 E (#24.5) to map |
| 20137 | | W20_J3623_consensus_structure.mrc (#1) using 1876 atoms |
| 20138 | | average map value = 0.352, steps = 52 |
| 20139 | | shifted from previous position = 0.551 |
| 20140 | | rotated from previous position = 2.49 degrees |
| 20141 | | atoms outside contour = 518, contour level = 0.25746 |
| 20142 | | |
| 20143 | | Position of COPI_cyto_for_J4027_v1 E (#24.5) relative to |
| 20144 | | W20_J3623_consensus_structure.mrc (#1) coordinates: |
| 20145 | | Matrix rotation and translation |
| 20146 | | -0.40106793 -0.89989636 -0.17126318 279.30609803 |
| 20147 | | -0.77603258 0.23443438 0.58550318 159.37835572 |
| 20148 | | -0.48674220 0.36773235 -0.79237300 240.44712243 |
| 20149 | | Axis -0.54056671 0.78310515 0.30746375 |
| 20150 | | Axis point 209.32932986 0.00000000 69.11212793 |
| 20151 | | Rotation angle (degrees) 168.37950772 |
| 20152 | | Shift along axis 47.75520439 |
| 20153 | | |
| 20154 | | |
| 20155 | | > fitmap #24.6 inMap #1 |
| 20156 | | |
| 20157 | | Fit molecule COPI_cyto_for_J4027_v1 F (#24.6) to map |
| 20158 | | W20_J3623_consensus_structure.mrc (#1) using 2400 atoms |
| 20159 | | average map value = 0.1995, steps = 32 |
| 20160 | | shifted from previous position = 0.88 |
| 20161 | | rotated from previous position = 2.72 degrees |
| 20162 | | atoms outside contour = 2236, contour level = 0.25746 |
| 20163 | | |
| 20164 | | Position of COPI_cyto_for_J4027_v1 F (#24.6) relative to |
| 20165 | | W20_J3623_consensus_structure.mrc (#1) coordinates: |
| 20166 | | Matrix rotation and translation |
| 20167 | | -0.34705513 -0.92807037 -0.13504869 272.53895474 |
| 20168 | | -0.78593629 0.20923862 0.58182761 162.32498204 |
| 20169 | | -0.51171956 0.30806592 -0.80202151 247.95535590 |
| 20170 | | Axis -0.56230557 0.77368067 0.29194291 |
| 20171 | | Axis point 214.01677259 0.00000000 69.57811076 |
| 20172 | | Rotation angle (degrees) 165.91105545 |
| 20173 | | Shift along axis 44.72633565 |
| 20174 | | |
| 20175 | | |
| 20176 | | > fitmap #24.7 inMap #1 |
| 20177 | | |
| 20178 | | Fit molecule COPI_cyto_for_J4027_v1 G (#24.7) to map |
| 20179 | | W20_J3623_consensus_structure.mrc (#1) using 2302 atoms |
| 20180 | | average map value = 0.2042, steps = 56 |
| 20181 | | shifted from previous position = 0.732 |
| 20182 | | rotated from previous position = 2.42 degrees |
| 20183 | | atoms outside contour = 1956, contour level = 0.25746 |
| 20184 | | |
| 20185 | | Position of COPI_cyto_for_J4027_v1 G (#24.7) relative to |
| 20186 | | W20_J3623_consensus_structure.mrc (#1) coordinates: |
| 20187 | | Matrix rotation and translation |
| 20188 | | -0.40089736 -0.90460310 -0.14482596 274.53357578 |
| 20189 | | -0.79035024 0.26156664 0.55401210 159.12795064 |
| 20190 | | -0.46327942 0.33656522 -0.81981464 242.17863151 |
| 20191 | | Axis -0.54066936 0.79181649 0.28408325 |
| 20192 | | Axis point 206.28449602 0.00000000 73.32567888 |
| 20193 | | Rotation angle (degrees) 168.39926749 |
| 20194 | | Shift along axis 46.36713644 |
| 20195 | | |
| 20196 | | |
| 20197 | | > fitmap #24.8 inMap #1 |
| 20198 | | |
| 20199 | | Fit molecule COPI_cyto_for_J4027_v1 H (#24.8) to map |
| 20200 | | W20_J3623_consensus_structure.mrc (#1) using 420 atoms |
| 20201 | | average map value = 0.2665, steps = 72 |
| 20202 | | shifted from previous position = 1.63 |
| 20203 | | rotated from previous position = 6.63 degrees |
| 20204 | | atoms outside contour = 172, contour level = 0.25746 |
| 20205 | | |
| 20206 | | Position of COPI_cyto_for_J4027_v1 H (#24.8) relative to |
| 20207 | | W20_J3623_consensus_structure.mrc (#1) coordinates: |
| 20208 | | Matrix rotation and translation |
| 20209 | | -0.32134690 -0.93539737 -0.14753964 274.57265625 |
| 20210 | | -0.85481390 0.21949425 0.47022919 174.99288921 |
| 20211 | | -0.40746704 0.27722563 -0.87012446 247.94129870 |
| 20212 | | Axis -0.57850272 0.77909809 0.24153833 |
| 20213 | | Axis point 215.13355776 0.00000000 80.87806975 |
| 20214 | | Rotation angle (degrees) 170.39742774 |
| 20215 | | Shift along axis 37.38292387 |
| 20216 | | |
| 20217 | | |
| 20218 | | > fitmap #24.9 inMap #1 |
| 20219 | | |
| 20220 | | Fit molecule COPI_cyto_for_J4027_v1 I (#24.9) to map |
| 20221 | | W20_J3623_consensus_structure.mrc (#1) using 1573 atoms |
| 20222 | | average map value = 0.3464, steps = 64 |
| 20223 | | shifted from previous position = 0.478 |
| 20224 | | rotated from previous position = 0.943 degrees |
| 20225 | | atoms outside contour = 463, contour level = 0.25746 |
| 20226 | | |
| 20227 | | Position of COPI_cyto_for_J4027_v1 I (#24.9) relative to |
| 20228 | | W20_J3623_consensus_structure.mrc (#1) coordinates: |
| 20229 | | Matrix rotation and translation |
| 20230 | | -0.36293127 -0.91488242 -0.17683625 275.64058476 |
| 20231 | | -0.80647349 0.21333854 0.55144102 166.30863857 |
| 20232 | | -0.46677771 0.34274894 -0.81525564 240.90103613 |
| 20233 | | Axis -0.55901039 0.77664797 0.29038821 |
| 20234 | | Axis point 211.48290973 0.00000000 71.12426755 |
| 20235 | | Rotation angle (degrees) 169.24194914 |
| 20236 | | Shift along axis 45.03213770 |
| 20237 | | |
| 20238 | | |
| 20239 | | > fitmap #24.10 inMap #1 |
| 20240 | | |
| 20241 | | Fit molecule COPI_cyto_for_J4027_v1 J (#24.10) to map |
| 20242 | | W20_J3623_consensus_structure.mrc (#1) using 1112 atoms |
| 20243 | | average map value = 0.3459, steps = 52 |
| 20244 | | shifted from previous position = 0.38 |
| 20245 | | rotated from previous position = 3.4 degrees |
| 20246 | | atoms outside contour = 351, contour level = 0.25746 |
| 20247 | | |
| 20248 | | Position of COPI_cyto_for_J4027_v1 J (#24.10) relative to |
| 20249 | | W20_J3623_consensus_structure.mrc (#1) coordinates: |
| 20250 | | Matrix rotation and translation |
| 20251 | | -0.36982106 -0.92265040 -0.10930983 270.39934908 |
| 20252 | | -0.79564879 0.25374928 0.55004938 163.22079540 |
| 20253 | | -0.47976598 0.29039208 -0.82794750 248.56734521 |
| 20254 | | Axis -0.55260110 0.78840254 0.27028405 |
| 20255 | | Axis point 210.23381150 0.00000000 73.93598950 |
| 20256 | | Rotation angle (degrees) 166.41185550 |
| 20257 | | Shift along axis 46.44450154 |
| 20258 | | |
| 20259 | | |
| 20260 | | > fitmap #24.11 inMap #1 |
| 20261 | | |
| 20262 | | Fit molecule COPI_cyto_for_J4027_v1 K (#24.11) to map |
| 20263 | | W20_J3623_consensus_structure.mrc (#1) using 319 atoms |
| 20264 | | average map value = 0.2981, steps = 324 |
| 20265 | | shifted from previous position = 19.5 |
| 20266 | | rotated from previous position = 32.5 degrees |
| 20267 | | atoms outside contour = 138, contour level = 0.25746 |
| 20268 | | |
| 20269 | | Position of COPI_cyto_for_J4027_v1 K (#24.11) relative to |
| 20270 | | W20_J3623_consensus_structure.mrc (#1) coordinates: |
| 20271 | | Matrix rotation and translation |
| 20272 | | -0.06351642 -0.99614584 0.06049082 250.07246282 |
| 20273 | | -0.53379297 0.08512482 0.84131971 112.08368631 |
| 20274 | | -0.84322639 0.02114804 -0.53714247 260.93738590 |
| 20275 | | Axis -0.62845977 0.69247687 0.35427971 |
| 20276 | | Axis point 244.61405927 0.00000000 32.59441219 |
| 20277 | | Rotation angle (degrees) 139.26773764 |
| 20278 | | Shift along axis 12.89969989 |
| 20279 | | |
| 20280 | | |
| 20281 | | > split #25 chains |
| 20282 | | |
| 20283 | | Split COPI_cyto_J3987_model (#25) into 13 models |
| 20284 | | Chain information for COPI_cyto_J3987_model A #25.1 |
| 20285 | | --- |
| 20286 | | Chain | Description |
| 20287 | | A | No description available |
| 20288 | | |
| 20289 | | Chain information for COPI_cyto_J3987_model B #25.2 |
| 20290 | | --- |
| 20291 | | Chain | Description |
| 20292 | | B | No description available |
| 20293 | | |
| 20294 | | Chain information for COPI_cyto_J3987_model C #25.3 |
| 20295 | | --- |
| 20296 | | Chain | Description |
| 20297 | | C | No description available |
| 20298 | | |
| 20299 | | Chain information for COPI_cyto_J3987_model D #25.4 |
| 20300 | | --- |
| 20301 | | Chain | Description |
| 20302 | | D | No description available |
| 20303 | | |
| 20304 | | Chain information for COPI_cyto_J3987_model E #25.5 |
| 20305 | | --- |
| 20306 | | Chain | Description |
| 20307 | | E | No description available |
| 20308 | | |
| 20309 | | Chain information for COPI_cyto_J3987_model F #25.6 |
| 20310 | | --- |
| 20311 | | Chain | Description |
| 20312 | | F | No description available |
| 20313 | | |
| 20314 | | Chain information for COPI_cyto_J3987_model G #25.7 |
| 20315 | | --- |
| 20316 | | Chain | Description |
| 20317 | | G | No description available |
| 20318 | | |
| 20319 | | Chain information for COPI_cyto_J3987_model H #25.8 |
| 20320 | | --- |
| 20321 | | Chain | Description |
| 20322 | | H | No description available |
| 20323 | | |
| 20324 | | Chain information for COPI_cyto_J3987_model I #25.9 |
| 20325 | | --- |
| 20326 | | Chain | Description |
| 20327 | | I | No description available |
| 20328 | | |
| 20329 | | Chain information for COPI_cyto_J3987_model J #25.10 |
| 20330 | | --- |
| 20331 | | Chain | Description |
| 20332 | | J | No description available |
| 20333 | | |
| 20334 | | Chain information for COPI_cyto_J3987_model K #25.11 |
| 20335 | | --- |
| 20336 | | Chain | Description |
| 20337 | | K | No description available |
| 20338 | | |
| 20339 | | Chain information for COPI_cyto_J3987_model L #25.12 |
| 20340 | | --- |
| 20341 | | Chain | Description |
| 20342 | | L | No description available |
| 20343 | | |
| 20344 | | Chain information for COPI_cyto_J3987_model M #25.13 |
| 20345 | | --- |
| 20346 | | Chain | Description |
| 20347 | | M | No description available |
| 20348 | | |
| 20349 | | |
| 20350 | | > hide #!1 models |
| 20351 | | |
| 20352 | | > show #!4 models |
| 20353 | | |
| 20354 | | > fitmap #25.1 inMap #4 |
| 20355 | | |
| 20356 | | Fit molecule COPI_cyto_J3987_model A (#25.1) to map |
| 20357 | | W20_J3987_consensus_resampled.mrc (#4) using 2590 atoms |
| 20358 | | average map value = 0.3221, steps = 80 |
| 20359 | | shifted from previous position = 0.776 |
| 20360 | | rotated from previous position = 1.16 degrees |
| 20361 | | atoms outside contour = 793, contour level = 0.27665 |
| 20362 | | |
| 20363 | | Position of COPI_cyto_J3987_model A (#25.1) relative to |
| 20364 | | W20_J3987_consensus_resampled.mrc (#4) coordinates: |
| 20365 | | Matrix rotation and translation |
| 20366 | | -0.38698629 -0.90565681 -0.17328409 278.18136001 |
| 20367 | | -0.79448095 0.23210684 0.56118308 163.47572576 |
| 20368 | | -0.46801885 0.35484106 -0.80934924 239.73459315 |
| 20369 | | Axis -0.54794991 0.78268057 0.29523216 |
| 20370 | | Axis point 210.03344096 0.00000000 70.61465245 |
| 20371 | | Rotation angle (degrees) 169.14725684 |
| 20372 | | Shift along axis 46.29718384 |
| 20373 | | |
| 20374 | | |
| 20375 | | > fitmap #25.2 inMap #4 |
| 20376 | | |
| 20377 | | Fit molecule COPI_cyto_J3987_model B (#25.2) to map |
| 20378 | | W20_J3987_consensus_resampled.mrc (#4) using 2426 atoms |
| 20379 | | average map value = 0.3153, steps = 72 |
| 20380 | | shifted from previous position = 0.76 |
| 20381 | | rotated from previous position = 2.24 degrees |
| 20382 | | atoms outside contour = 837, contour level = 0.27665 |
| 20383 | | |
| 20384 | | Position of COPI_cyto_J3987_model B (#25.2) relative to |
| 20385 | | W20_J3987_consensus_resampled.mrc (#4) coordinates: |
| 20386 | | Matrix rotation and translation |
| 20387 | | -0.39446310 -0.89902146 -0.19015597 280.33015308 |
| 20388 | | -0.79147162 0.22726378 0.56738336 162.70699196 |
| 20389 | | -0.46687424 0.37431485 -0.80119714 238.32585966 |
| 20390 | | Axis -0.54517505 0.78138014 0.30369267 |
| 20391 | | Axis point 209.72584131 0.00000000 70.08251673 |
| 20392 | | Rotation angle (degrees) 169.80084660 |
| 20393 | | Shift along axis 46.68482501 |
| 20394 | | |
| 20395 | | |
| 20396 | | > fitmap #25.3 inMap #4 |
| 20397 | | |
| 20398 | | Fit molecule COPI_cyto_J3987_model C (#25.3) to map |
| 20399 | | W20_J3987_consensus_resampled.mrc (#4) using 1612 atoms |
| 20400 | | average map value = 0.3176, steps = 88 |
| 20401 | | shifted from previous position = 0.713 |
| 20402 | | rotated from previous position = 1.99 degrees |
| 20403 | | atoms outside contour = 638, contour level = 0.27665 |
| 20404 | | |
| 20405 | | Position of COPI_cyto_J3987_model C (#25.3) relative to |
| 20406 | | W20_J3987_consensus_resampled.mrc (#4) coordinates: |
| 20407 | | Matrix rotation and translation |
| 20408 | | -0.39593352 -0.89983916 -0.18310146 278.71276949 |
| 20409 | | -0.79448375 0.23569092 0.55968328 163.24766694 |
| 20410 | | -0.46046958 0.36706850 -0.80822553 238.88829135 |
| 20411 | | Axis -0.54450663 0.78409758 0.29783135 |
| 20412 | | Axis point 208.81546281 0.00000000 71.13107453 |
| 20413 | | Rotation angle (degrees) 169.81244748 |
| 20414 | | Shift along axis 47.38957070 |
| 20415 | | |
| 20416 | | |
| 20417 | | > fitmap #25.4 inMap #4 |
| 20418 | | |
| 20419 | | Fit molecule COPI_cyto_J3987_model D (#25.4) to map |
| 20420 | | W20_J3987_consensus_resampled.mrc (#4) using 4745 atoms |
| 20421 | | average map value = 0.3393, steps = 64 |
| 20422 | | shifted from previous position = 0.187 |
| 20423 | | rotated from previous position = 0.91 degrees |
| 20424 | | atoms outside contour = 1720, contour level = 0.27665 |
| 20425 | | |
| 20426 | | Position of COPI_cyto_J3987_model D (#25.4) relative to |
| 20427 | | W20_J3987_consensus_resampled.mrc (#4) coordinates: |
| 20428 | | Matrix rotation and translation |
| 20429 | | -0.36779496 -0.91688768 -0.15506087 274.91861534 |
| 20430 | | -0.80287248 0.22897373 0.55042423 165.38152260 |
| 20431 | | -0.46917233 0.32693736 -0.82035926 243.65468775 |
| 20432 | | Axis -0.55592398 0.78135279 0.28361301 |
| 20433 | | Axis point 211.28631778 0.00000000 72.30612718 |
| 20434 | | Rotation angle (degrees) 168.40427173 |
| 20435 | | Shift along axis 45.49110059 |
| 20436 | | |
| 20437 | | |
| 20438 | | > hide #!95 models |
| 20439 | | |
| 20440 | | > show #25.1 models |
| 20441 | | |
| 20442 | | > show #25.2 models |
| 20443 | | |
| 20444 | | > show #25.3 models |
| 20445 | | |
| 20446 | | > show #!25.4 models |
| 20447 | | |
| 20448 | | > show #25.5 models |
| 20449 | | |
| 20450 | | > show #25.6 models |
| 20451 | | |
| 20452 | | > show #25.7 models |
| 20453 | | |
| 20454 | | > show #25.8 models |
| 20455 | | |
| 20456 | | > show #25.9 models |
| 20457 | | |
| 20458 | | > show #25.10 models |
| 20459 | | |
| 20460 | | > show #25.11 models |
| 20461 | | |
| 20462 | | > show #25.12 models |
| 20463 | | |
| 20464 | | > show #25.13 models |
| 20465 | | |
| 20466 | | > fitmap #25.5 inMap #4 |
| 20467 | | |
| 20468 | | Fit molecule COPI_cyto_J3987_model E (#25.5) to map |
| 20469 | | W20_J3987_consensus_resampled.mrc (#4) using 1876 atoms |
| 20470 | | average map value = 0.3141, steps = 60 |
| 20471 | | shifted from previous position = 0.493 |
| 20472 | | rotated from previous position = 0.732 degrees |
| 20473 | | atoms outside contour = 674, contour level = 0.27665 |
| 20474 | | |
| 20475 | | Position of COPI_cyto_J3987_model E (#25.5) relative to |
| 20476 | | W20_J3987_consensus_resampled.mrc (#4) coordinates: |
| 20477 | | Matrix rotation and translation |
| 20478 | | -0.38331200 -0.90908551 -0.16320372 277.20038029 |
| 20479 | | -0.79186725 0.23250965 0.56469952 162.86818551 |
| 20480 | | -0.47541371 0.34569178 -0.80899877 242.05560365 |
| 20481 | | Axis -0.54892849 0.78253380 0.29379988 |
| 20482 | | Axis point 210.42960898 0.00000000 70.96821393 |
| 20483 | | Rotation angle (degrees) 168.49306276 |
| 20484 | | Shift along axis 46.40258047 |
| 20485 | | |
| 20486 | | |
| 20487 | | > fitmap #25.6 inMap #4 |
| 20488 | | |
| 20489 | | Fit molecule COPI_cyto_J3987_model F (#25.6) to map |
| 20490 | | W20_J3987_consensus_resampled.mrc (#4) using 2400 atoms |
| 20491 | | average map value = 0.2626, steps = 52 |
| 20492 | | shifted from previous position = 2.64 |
| 20493 | | rotated from previous position = 2.91 degrees |
| 20494 | | atoms outside contour = 1308, contour level = 0.27665 |
| 20495 | | |
| 20496 | | Position of COPI_cyto_J3987_model F (#25.6) relative to |
| 20497 | | W20_J3987_consensus_resampled.mrc (#4) coordinates: |
| 20498 | | Matrix rotation and translation |
| 20499 | | -0.40208359 -0.90303670 -0.15117378 274.51102549 |
| 20500 | | -0.76578539 0.24117028 0.59616243 157.61497455 |
| 20501 | | -0.50189793 0.35547381 -0.78850292 240.80443864 |
| 20502 | | Axis -0.53849728 0.78468186 0.30707499 |
| 20503 | | Axis point 207.60053656 0.00000000 67.83219255 |
| 20504 | | Rotation angle (degrees) 167.08638464 |
| 20505 | | Shift along axis 49.79919045 |
| 20506 | | |
| 20507 | | |
| 20508 | | > fitmap #25.7 inMap #4 |
| 20509 | | |
| 20510 | | Fit molecule COPI_cyto_J3987_model G (#25.7) to map |
| 20511 | | W20_J3987_consensus_resampled.mrc (#4) using 2302 atoms |
| 20512 | | average map value = 0.2759, steps = 68 |
| 20513 | | shifted from previous position = 1.66 |
| 20514 | | rotated from previous position = 2.11 degrees |
| 20515 | | atoms outside contour = 1125, contour level = 0.27665 |
| 20516 | | |
| 20517 | | Position of COPI_cyto_J3987_model G (#25.7) relative to |
| 20518 | | W20_J3987_consensus_resampled.mrc (#4) coordinates: |
| 20519 | | Matrix rotation and translation |
| 20520 | | -0.37470678 -0.90717007 -0.19140875 277.62743359 |
| 20521 | | -0.79108106 0.20516374 0.57627998 166.08595678 |
| 20522 | | -0.48351381 0.36735586 -0.79452130 238.82922597 |
| 20523 | | Axis -0.55354811 0.77393742 0.30757985 |
| 20524 | | Axis point 211.98563964 0.00000000 67.68895792 |
| 20525 | | Rotation angle (degrees) 169.12227850 |
| 20526 | | Shift along axis 48.31905400 |
| 20527 | | |
| 20528 | | |
| 20529 | | > fitmap #25.8 inMap #4 |
| 20530 | | |
| 20531 | | Fit molecule COPI_cyto_J3987_model H (#25.8) to map |
| 20532 | | W20_J3987_consensus_resampled.mrc (#4) using 420 atoms |
| 20533 | | average map value = 0.3391, steps = 52 |
| 20534 | | shifted from previous position = 1.47 |
| 20535 | | rotated from previous position = 4.51 degrees |
| 20536 | | atoms outside contour = 138, contour level = 0.27665 |
| 20537 | | |
| 20538 | | Position of COPI_cyto_J3987_model H (#25.8) relative to |
| 20539 | | W20_J3987_consensus_resampled.mrc (#4) coordinates: |
| 20540 | | Matrix rotation and translation |
| 20541 | | -0.30273203 -0.93833428 -0.16697938 274.74649750 |
| 20542 | | -0.83167276 0.17451603 0.52712864 176.16874621 |
| 20543 | | -0.46548229 0.29845092 -0.83321864 248.80226785 |
| 20544 | | Axis -0.58505795 0.76370144 0.27288698 |
| 20545 | | Axis point 220.19027093 0.00000000 73.60291153 |
| 20546 | | Rotation angle (degrees) 168.73005781 |
| 20547 | | Shift along axis 41.69260200 |
| 20548 | | |
| 20549 | | |
| 20550 | | > fitmap #25.9 inMap #4 |
| 20551 | | |
| 20552 | | Fit molecule COPI_cyto_J3987_model I (#25.9) to map |
| 20553 | | W20_J3987_consensus_resampled.mrc (#4) using 1573 atoms |
| 20554 | | average map value = 0.3297, steps = 60 |
| 20555 | | shifted from previous position = 0.422 |
| 20556 | | rotated from previous position = 0.637 degrees |
| 20557 | | atoms outside contour = 541, contour level = 0.27665 |
| 20558 | | |
| 20559 | | Position of COPI_cyto_J3987_model I (#25.9) relative to |
| 20560 | | W20_J3987_consensus_resampled.mrc (#4) coordinates: |
| 20561 | | Matrix rotation and translation |
| 20562 | | -0.37486313 -0.91082017 -0.17287068 276.29172892 |
| 20563 | | -0.79723061 0.22153165 0.56155775 164.41773716 |
| 20564 | | -0.47318179 0.34832509 -0.80917775 241.63479140 |
| 20565 | | Axis -0.55323087 0.77915540 0.29470744 |
| 20566 | | Axis point 210.74543880 0.00000000 70.87207510 |
| 20567 | | Rotation angle (degrees) 168.88868220 |
| 20568 | | Shift along axis 46.46542504 |
| 20569 | | |
| 20570 | | |
| 20571 | | > fitmap #25.10 inMap #4 |
| 20572 | | |
| 20573 | | Fit molecule COPI_cyto_J3987_model J (#25.10) to map |
| 20574 | | W20_J3987_consensus_resampled.mrc (#4) using 1112 atoms |
| 20575 | | average map value = 0.3174, steps = 68 |
| 20576 | | shifted from previous position = 0.699 |
| 20577 | | rotated from previous position = 4.11 degrees |
| 20578 | | atoms outside contour = 446, contour level = 0.27665 |
| 20579 | | |
| 20580 | | Position of COPI_cyto_J3987_model J (#25.10) relative to |
| 20581 | | W20_J3987_consensus_resampled.mrc (#4) coordinates: |
| 20582 | | Matrix rotation and translation |
| 20583 | | -0.37167949 -0.92301396 -0.09949677 269.60077643 |
| 20584 | | -0.80025326 0.26421818 0.53831543 164.62224104 |
| 20585 | | -0.47058379 0.27970342 -0.83684940 249.14141931 |
| 20586 | | Axis -0.55179431 0.79177956 0.26193160 |
| 20587 | | Axis point 209.93718651 0.00000000 75.17215989 |
| 20588 | | Rotation angle (degrees) 166.44743618 |
| 20589 | | Shift along axis 46.83836222 |
| 20590 | | |
| 20591 | | |
| 20592 | | > fitmap #25.11 inMap #4 |
| 20593 | | |
| 20594 | | Fit molecule COPI_cyto_J3987_model K (#25.11) to map |
| 20595 | | W20_J3987_consensus_resampled.mrc (#4) using 319 atoms |
| 20596 | | average map value = 0.3247, steps = 352 |
| 20597 | | shifted from previous position = 17.9 |
| 20598 | | rotated from previous position = 29.5 degrees |
| 20599 | | atoms outside contour = 122, contour level = 0.27665 |
| 20600 | | |
| 20601 | | Position of COPI_cyto_J3987_model K (#25.11) relative to |
| 20602 | | W20_J3987_consensus_resampled.mrc (#4) coordinates: |
| 20603 | | Matrix rotation and translation |
| 20604 | | -0.11943694 -0.99009806 0.07376081 250.46546690 |
| 20605 | | -0.55633477 0.12827455 0.82099774 113.52890835 |
| 20606 | | -0.82232990 0.05702176 -0.56614669 259.81159734 |
| 20607 | | Axis -0.60879152 0.71407012 0.34565408 |
| 20608 | | Axis point 236.23455332 0.00000000 37.79456885 |
| 20609 | | Rotation angle (degrees) 141.13755065 |
| 20610 | | Shift along axis 18.39128867 |
| 20611 | | |
| 20612 | | |
| 20613 | | > select add #25.11 |
| 20614 | | |
| 20615 | | 319 atoms, 320 bonds, 37 residues, 1 model selected |
| 20616 | | |
| 20617 | | > view matrix models |
| 20618 | | > #25.11,-0.13737,-0.98867,0.060447,253.17,-0.58792,0.1305,0.79832,118.22,-0.79717,0.074131,-0.59919,261.16 |
| 20619 | | |
| 20620 | | > ui tool show Matchmaker |
| 20621 | | |
| 20622 | | > matchmaker #25.11 to #26 |
| 20623 | | |
| 20624 | | Parameters |
| 20625 | | --- |
| 20626 | | Chain pairing | bb |
| 20627 | | Alignment algorithm | Needleman-Wunsch |
| 20628 | | Similarity matrix | BLOSUM-62 |
| 20629 | | SS fraction | 0.3 |
| 20630 | | Gap open (HH/SS/other) | 18/18/6 |
| 20631 | | Gap extend | 1 |
| 20632 | | SS matrix | | | H | S | O |
| 20633 | | ---|---|---|--- |
| 20634 | | H | 6 | -9 | -6 |
| 20635 | | S | | 6 | -6 |
| 20636 | | O | | | 4 |
| 20637 | | Iteration cutoff | 2 |
| 20638 | | |
| 20639 | | Matchmaker COPI_cyto_J4021_model, chain K (#26) with COPI_cyto_J3987_model K, |
| 20640 | | chain K (#25.11), sequence alignment score = 192.1 |
| 20641 | | RMSD between 37 pruned atom pairs is 0.000 angstroms; (across all 37 pairs: |
| 20642 | | 0.000) |
| 20643 | | |
| 20644 | | |
| 20645 | | > select subtract #25.11 |
| 20646 | | |
| 20647 | | Nothing selected |
| 20648 | | |
| 20649 | | > hide #!25 models |
| 20650 | | |
| 20651 | | > show #!26 models |
| 20652 | | |
| 20653 | | > split #26 chains |
| 20654 | | |
| 20655 | | Split COPI_cyto_J4021_model (#26) into 13 models |
| 20656 | | Chain information for COPI_cyto_J4021_model A #26.1 |
| 20657 | | --- |
| 20658 | | Chain | Description |
| 20659 | | A | No description available |
| 20660 | | |
| 20661 | | Chain information for COPI_cyto_J4021_model B #26.2 |
| 20662 | | --- |
| 20663 | | Chain | Description |
| 20664 | | B | No description available |
| 20665 | | |
| 20666 | | Chain information for COPI_cyto_J4021_model C #26.3 |
| 20667 | | --- |
| 20668 | | Chain | Description |
| 20669 | | C | No description available |
| 20670 | | |
| 20671 | | Chain information for COPI_cyto_J4021_model D #26.4 |
| 20672 | | --- |
| 20673 | | Chain | Description |
| 20674 | | D | No description available |
| 20675 | | |
| 20676 | | Chain information for COPI_cyto_J4021_model E #26.5 |
| 20677 | | --- |
| 20678 | | Chain | Description |
| 20679 | | E | No description available |
| 20680 | | |
| 20681 | | Chain information for COPI_cyto_J4021_model F #26.6 |
| 20682 | | --- |
| 20683 | | Chain | Description |
| 20684 | | F | No description available |
| 20685 | | |
| 20686 | | Chain information for COPI_cyto_J4021_model G #26.7 |
| 20687 | | --- |
| 20688 | | Chain | Description |
| 20689 | | G | No description available |
| 20690 | | |
| 20691 | | Chain information for COPI_cyto_J4021_model H #26.8 |
| 20692 | | --- |
| 20693 | | Chain | Description |
| 20694 | | H | No description available |
| 20695 | | |
| 20696 | | Chain information for COPI_cyto_J4021_model I #26.9 |
| 20697 | | --- |
| 20698 | | Chain | Description |
| 20699 | | I | No description available |
| 20700 | | |
| 20701 | | Chain information for COPI_cyto_J4021_model J #26.10 |
| 20702 | | --- |
| 20703 | | Chain | Description |
| 20704 | | J | No description available |
| 20705 | | |
| 20706 | | Chain information for COPI_cyto_J4021_model K #26.11 |
| 20707 | | --- |
| 20708 | | Chain | Description |
| 20709 | | K | No description available |
| 20710 | | |
| 20711 | | Chain information for COPI_cyto_J4021_model L #26.12 |
| 20712 | | --- |
| 20713 | | Chain | Description |
| 20714 | | L | No description available |
| 20715 | | |
| 20716 | | Chain information for COPI_cyto_J4021_model M #26.13 |
| 20717 | | --- |
| 20718 | | Chain | Description |
| 20719 | | M | No description available |
| 20720 | | |
| 20721 | | |
| 20722 | | > hide #!4 models |
| 20723 | | |
| 20724 | | > show #!6 models |
| 20725 | | |
| 20726 | | > fitmap #26.1 inMap #6 |
| 20727 | | |
| 20728 | | Fit molecule COPI_cyto_J4021_model A (#26.1) to map |
| 20729 | | W20_J4021_consensus_resampled.mrc (#6) using 2590 atoms |
| 20730 | | average map value = 0.2703, steps = 84 |
| 20731 | | shifted from previous position = 0.378 |
| 20732 | | rotated from previous position = 1.1 degrees |
| 20733 | | atoms outside contour = 937, contour level = 0.25941 |
| 20734 | | |
| 20735 | | Position of COPI_cyto_J4021_model A (#26.1) relative to |
| 20736 | | W20_J4021_consensus_resampled.mrc (#6) coordinates: |
| 20737 | | Matrix rotation and translation |
| 20738 | | -0.37414892 -0.91220652 -0.16700861 276.47462167 |
| 20739 | | -0.79157778 0.22032122 0.56996772 162.80224157 |
| 20740 | | -0.48313272 0.34545310 -0.80451534 242.25699208 |
| 20741 | | Axis -0.55289681 0.77849727 0.29706416 |
| 20742 | | Axis point 211.11351512 0.00000000 70.18074399 |
| 20743 | | Rotation angle (degrees) 168.28551633 |
| 20744 | | Shift along axis 45.84503360 |
| 20745 | | |
| 20746 | | |
| 20747 | | > fitmap #26.2 inMap #6 |
| 20748 | | |
| 20749 | | Fit molecule COPI_cyto_J4021_model B (#26.2) to map |
| 20750 | | W20_J4021_consensus_resampled.mrc (#6) using 2426 atoms |
| 20751 | | average map value = 0.2468, steps = 64 |
| 20752 | | shifted from previous position = 0.762 |
| 20753 | | rotated from previous position = 1.85 degrees |
| 20754 | | atoms outside contour = 1379, contour level = 0.25941 |
| 20755 | | |
| 20756 | | Position of COPI_cyto_J4021_model B (#26.2) relative to |
| 20757 | | W20_J4021_consensus_resampled.mrc (#6) coordinates: |
| 20758 | | Matrix rotation and translation |
| 20759 | | -0.37049992 -0.90946831 -0.18867225 277.51418546 |
| 20760 | | -0.80121018 0.21016989 0.56025966 165.92440083 |
| 20761 | | -0.46988517 0.35874228 -0.80654319 240.14558920 |
| 20762 | | Axis -0.55590253 0.77574936 0.29863910 |
| 20763 | | Axis point 211.54042372 0.00000000 70.27796042 |
| 20764 | | Rotation angle (degrees) 169.55728062 |
| 20765 | | Shift along axis 46.16177391 |
| 20766 | | |
| 20767 | | |
| 20768 | | > fitmap #26.3 inMap #6 |
| 20769 | | |
| 20770 | | Fit molecule COPI_cyto_J4021_model C (#26.3) to map |
| 20771 | | W20_J4021_consensus_resampled.mrc (#6) using 1612 atoms |
| 20772 | | average map value = 0.268, steps = 88 |
| 20773 | | shifted from previous position = 1.42 |
| 20774 | | rotated from previous position = 8.83 degrees |
| 20775 | | atoms outside contour = 658, contour level = 0.25941 |
| 20776 | | |
| 20777 | | Position of COPI_cyto_J4021_model C (#26.3) relative to |
| 20778 | | W20_J4021_consensus_resampled.mrc (#6) coordinates: |
| 20779 | | Matrix rotation and translation |
| 20780 | | -0.45746641 -0.88778349 -0.05064549 275.77079696 |
| 20781 | | -0.69608083 0.32207858 0.64166727 143.83899735 |
| 20782 | | -0.55334978 0.32879458 -0.76530919 246.76254892 |
| 20783 | | Axis -0.50270755 0.80771908 0.30801786 |
| 20784 | | Axis point 205.38970331 0.00000000 66.05855727 |
| 20785 | | Rotation angle (degrees) 161.86918570 |
| 20786 | | Shift along axis 53.55671210 |
| 20787 | | |
| 20788 | | |
| 20789 | | > fitmap #26.4 inMap #6 |
| 20790 | | |
| 20791 | | Fit molecule COPI_cyto_J4021_model D (#26.4) to map |
| 20792 | | W20_J4021_consensus_resampled.mrc (#6) using 4745 atoms |
| 20793 | | average map value = 0.3102, steps = 84 |
| 20794 | | shifted from previous position = 1.18 |
| 20795 | | rotated from previous position = 1.93 degrees |
| 20796 | | atoms outside contour = 1692, contour level = 0.25941 |
| 20797 | | |
| 20798 | | Position of COPI_cyto_J4021_model D (#26.4) relative to |
| 20799 | | W20_J4021_consensus_resampled.mrc (#6) coordinates: |
| 20800 | | Matrix rotation and translation |
| 20801 | | -0.36883139 -0.91668768 -0.15377619 275.04474343 |
| 20802 | | -0.80433110 0.23185065 0.54708023 165.03207524 |
| 20803 | | -0.46584859 0.32546733 -0.82283420 243.51890015 |
| 20804 | | Axis -0.55555262 0.78232196 0.28166228 |
| 20805 | | Axis point 210.98432459 0.00000000 72.73645940 |
| 20806 | | Rotation angle (degrees) 168.49504583 |
| 20807 | | Shift along axis 44.89647919 |
| 20808 | | |
| 20809 | | |
| 20810 | | > fitmap #26.5 inMap #6 |
| 20811 | | |
| 20812 | | Fit molecule COPI_cyto_J4021_model E (#26.5) to map |
| 20813 | | W20_J4021_consensus_resampled.mrc (#6) using 1876 atoms |
| 20814 | | average map value = 0.2655, steps = 44 |
| 20815 | | shifted from previous position = 0.37 |
| 20816 | | rotated from previous position = 1.44 degrees |
| 20817 | | atoms outside contour = 812, contour level = 0.25941 |
| 20818 | | |
| 20819 | | Position of COPI_cyto_J4021_model E (#26.5) relative to |
| 20820 | | W20_J4021_consensus_resampled.mrc (#6) coordinates: |
| 20821 | | Matrix rotation and translation |
| 20822 | | -0.37950572 -0.91098051 -0.16152380 276.55156047 |
| 20823 | | -0.78154710 0.22222838 0.58292255 160.94019650 |
| 20824 | | -0.49513590 0.34746090 -0.79631111 243.08456807 |
| 20825 | | Axis -0.54968179 0.77881257 0.30216041 |
| 20826 | | Axis point 210.99674193 0.00000000 69.29658337 |
| 20827 | | Rotation angle (degrees) 167.63257816 |
| 20828 | | Shift along axis 46.77742440 |
| 20829 | | |
| 20830 | | |
| 20831 | | > fitmap #26.6 inMap #6 |
| 20832 | | |
| 20833 | | Fit molecule COPI_cyto_J4021_model F (#26.6) to map |
| 20834 | | W20_J4021_consensus_resampled.mrc (#6) using 2400 atoms |
| 20835 | | average map value = 0.3003, steps = 72 |
| 20836 | | shifted from previous position = 3.77 |
| 20837 | | rotated from previous position = 7.24 degrees |
| 20838 | | atoms outside contour = 749, contour level = 0.25941 |
| 20839 | | |
| 20840 | | Position of COPI_cyto_J4021_model F (#26.6) relative to |
| 20841 | | W20_J4021_consensus_resampled.mrc (#6) coordinates: |
| 20842 | | Matrix rotation and translation |
| 20843 | | -0.50121599 -0.84817893 -0.17139150 277.75765218 |
| 20844 | | -0.73837669 0.31593590 0.59580566 148.29415474 |
| 20845 | | -0.45120108 0.42517881 -0.78462767 230.60293751 |
| 20846 | | Axis -0.49366217 0.80955254 0.31768278 |
| 20847 | | Axis point 195.53368048 0.00000000 69.77740358 |
| 20848 | | Rotation angle (degrees) 170.04832921 |
| 20849 | | Shift along axis 56.19204834 |
| 20850 | | |
| 20851 | | |
| 20852 | | > fitmap #26.7 inMap #6 |
| 20853 | | |
| 20854 | | Fit molecule COPI_cyto_J4021_model G (#26.7) to map |
| 20855 | | W20_J4021_consensus_resampled.mrc (#6) using 2302 atoms |
| 20856 | | average map value = 0.3136, steps = 76 |
| 20857 | | shifted from previous position = 3.03 |
| 20858 | | rotated from previous position = 3.18 degrees |
| 20859 | | atoms outside contour = 723, contour level = 0.25941 |
| 20860 | | |
| 20861 | | Position of COPI_cyto_J4021_model G (#26.7) relative to |
| 20862 | | W20_J4021_consensus_resampled.mrc (#6) coordinates: |
| 20863 | | Matrix rotation and translation |
| 20864 | | -0.43399059 -0.88593113 -0.16364048 278.30290158 |
| 20865 | | -0.75953045 0.26210802 0.59532587 156.65606797 |
| 20866 | | -0.48452624 0.38265575 -0.78664408 237.63722363 |
| 20867 | | Axis -0.52487405 0.79195238 0.31195938 |
| 20868 | | Axis point 205.37019802 0.00000000 68.21146617 |
| 20869 | | Rotation angle (degrees) 168.31145195 |
| 20870 | | Shift along axis 52.12333508 |
| 20871 | | |
| 20872 | | |
| 20873 | | > fitmap #26.8 inMap #6 |
| 20874 | | |
| 20875 | | Fit molecule COPI_cyto_J4021_model H (#26.8) to map |
| 20876 | | W20_J4021_consensus_resampled.mrc (#6) using 420 atoms |
| 20877 | | average map value = 0.4011, steps = 52 |
| 20878 | | shifted from previous position = 2.32 |
| 20879 | | rotated from previous position = 1.55 degrees |
| 20880 | | atoms outside contour = 112, contour level = 0.25941 |
| 20881 | | |
| 20882 | | Position of COPI_cyto_J4021_model H (#26.8) relative to |
| 20883 | | W20_J4021_consensus_resampled.mrc (#6) coordinates: |
| 20884 | | Matrix rotation and translation |
| 20885 | | -0.41519620 -0.89510830 -0.16246005 278.00922432 |
| 20886 | | -0.77921085 0.25774528 0.57131239 160.68655904 |
| 20887 | | -0.46951315 0.36379736 -0.80449295 240.32587092 |
| 20888 | | Axis -0.53442036 0.79076406 0.29847457 |
| 20889 | | Axis point 207.26688590 0.00000000 70.94580099 |
| 20890 | | Rotation angle (degrees) 168.80494855 |
| 20891 | | Shift along axis 50.22252640 |
| 20892 | | |
| 20893 | | |
| 20894 | | > fitmap #26.9 inMap #6 |
| 20895 | | |
| 20896 | | Fit molecule COPI_cyto_J4021_model I (#26.9) to map |
| 20897 | | W20_J4021_consensus_resampled.mrc (#6) using 1573 atoms |
| 20898 | | average map value = 0.2888, steps = 112 |
| 20899 | | shifted from previous position = 1.44 |
| 20900 | | rotated from previous position = 3.06 degrees |
| 20901 | | atoms outside contour = 702, contour level = 0.25941 |
| 20902 | | |
| 20903 | | Position of COPI_cyto_J4021_model I (#26.9) relative to |
| 20904 | | W20_J4021_consensus_resampled.mrc (#6) coordinates: |
| 20905 | | Matrix rotation and translation |
| 20906 | | -0.37846625 -0.91010040 -0.16876189 276.33040233 |
| 20907 | | -0.76836367 0.20723976 0.60553197 162.64450565 |
| 20908 | | -0.51612071 0.35884392 -0.77772133 243.67694950 |
| 20909 | | Axis -0.54941463 0.77362488 0.31567087 |
| 20910 | | Axis point 212.64433272 0.00000000 66.29298345 |
| 20911 | | Rotation angle (degrees) 167.02647454 |
| 20912 | | Shift along axis 50.92758584 |
| 20913 | | |
| 20914 | | |
| 20915 | | > fitmap #26.10 inMap #6 |
| 20916 | | |
| 20917 | | Fit molecule COPI_cyto_J4021_model J (#26.10) to map |
| 20918 | | W20_J4021_consensus_resampled.mrc (#6) using 1112 atoms |
| 20919 | | average map value = 0.3374, steps = 68 |
| 20920 | | shifted from previous position = 0.981 |
| 20921 | | rotated from previous position = 4.81 degrees |
| 20922 | | atoms outside contour = 408, contour level = 0.25941 |
| 20923 | | |
| 20924 | | Position of COPI_cyto_J4021_model J (#26.10) relative to |
| 20925 | | W20_J4021_consensus_resampled.mrc (#6) coordinates: |
| 20926 | | Matrix rotation and translation |
| 20927 | | -0.36533925 -0.92641223 -0.09103639 268.10331410 |
| 20928 | | -0.79596275 0.26018434 0.54657793 163.12428402 |
| 20929 | | -0.48267023 0.27214795 -0.83244517 250.25943922 |
| 20930 | | Axis -0.55363524 0.79008238 0.26316888 |
| 20931 | | Axis point 210.17105433 0.00000000 74.56081239 |
| 20932 | | Rotation angle (degrees) 165.65005163 |
| 20933 | | Shift along axis 46.31067629 |
| 20934 | | |
| 20935 | | |
| 20936 | | > hide #!26 models |
| 20937 | | |
| 20938 | | > split #27 chains |
| 20939 | | |
| 20940 | | Split COPI_cyto_J4022_model (#27) into 13 models |
| 20941 | | Chain information for COPI_cyto_J4022_model A #27.1 |
| 20942 | | --- |
| 20943 | | Chain | Description |
| 20944 | | A | No description available |
| 20945 | | |
| 20946 | | Chain information for COPI_cyto_J4022_model B #27.2 |
| 20947 | | --- |
| 20948 | | Chain | Description |
| 20949 | | B | No description available |
| 20950 | | |
| 20951 | | Chain information for COPI_cyto_J4022_model C #27.3 |
| 20952 | | --- |
| 20953 | | Chain | Description |
| 20954 | | C | No description available |
| 20955 | | |
| 20956 | | Chain information for COPI_cyto_J4022_model D #27.4 |
| 20957 | | --- |
| 20958 | | Chain | Description |
| 20959 | | D | No description available |
| 20960 | | |
| 20961 | | Chain information for COPI_cyto_J4022_model E #27.5 |
| 20962 | | --- |
| 20963 | | Chain | Description |
| 20964 | | E | No description available |
| 20965 | | |
| 20966 | | Chain information for COPI_cyto_J4022_model F #27.6 |
| 20967 | | --- |
| 20968 | | Chain | Description |
| 20969 | | F | No description available |
| 20970 | | |
| 20971 | | Chain information for COPI_cyto_J4022_model G #27.7 |
| 20972 | | --- |
| 20973 | | Chain | Description |
| 20974 | | G | No description available |
| 20975 | | |
| 20976 | | Chain information for COPI_cyto_J4022_model H #27.8 |
| 20977 | | --- |
| 20978 | | Chain | Description |
| 20979 | | H | No description available |
| 20980 | | |
| 20981 | | Chain information for COPI_cyto_J4022_model I #27.9 |
| 20982 | | --- |
| 20983 | | Chain | Description |
| 20984 | | I | No description available |
| 20985 | | |
| 20986 | | Chain information for COPI_cyto_J4022_model J #27.10 |
| 20987 | | --- |
| 20988 | | Chain | Description |
| 20989 | | J | No description available |
| 20990 | | |
| 20991 | | Chain information for COPI_cyto_J4022_model K #27.11 |
| 20992 | | --- |
| 20993 | | Chain | Description |
| 20994 | | K | No description available |
| 20995 | | |
| 20996 | | Chain information for COPI_cyto_J4022_model L #27.12 |
| 20997 | | --- |
| 20998 | | Chain | Description |
| 20999 | | L | No description available |
| 21000 | | |
| 21001 | | Chain information for COPI_cyto_J4022_model M #27.13 |
| 21002 | | --- |
| 21003 | | Chain | Description |
| 21004 | | M | No description available |
| 21005 | | |
| 21006 | | |
| 21007 | | > hide #!6 models |
| 21008 | | |
| 21009 | | > show #!8 models |
| 21010 | | |
| 21011 | | > color #8 darkgrey models |
| 21012 | | |
| 21013 | | > color #8 #a9a9a9c0 models |
| 21014 | | |
| 21015 | | > color #8 #a9a9a9bf models |
| 21016 | | |
| 21017 | | > hide #!27 models |
| 21018 | | |
| 21019 | | > show #!27 models |
| 21020 | | |
| 21021 | | > show #27.1 models |
| 21022 | | |
| 21023 | | > show #27.2 models |
| 21024 | | |
| 21025 | | > show #27.3 models |
| 21026 | | |
| 21027 | | > show #!27.4 models |
| 21028 | | |
| 21029 | | > show #27.5 models |
| 21030 | | |
| 21031 | | > show #27.6 models |
| 21032 | | |
| 21033 | | > show #27.7 models |
| 21034 | | |
| 21035 | | > show #27.8 models |
| 21036 | | |
| 21037 | | > show #27.9 models |
| 21038 | | |
| 21039 | | > show #27.10 models |
| 21040 | | |
| 21041 | | > show #27.11 models |
| 21042 | | |
| 21043 | | > show #27.12 models |
| 21044 | | |
| 21045 | | > show #27.13 models |
| 21046 | | |
| 21047 | | > fitmap #27.1 inMap #8 |
| 21048 | | |
| 21049 | | Fit molecule COPI_cyto_J4022_model A (#27.1) to map |
| 21050 | | W20_J4022_consensus_resampled.mrc (#8) using 2590 atoms |
| 21051 | | average map value = 0.2646, steps = 56 |
| 21052 | | shifted from previous position = 1.25 |
| 21053 | | rotated from previous position = 2.42 degrees |
| 21054 | | atoms outside contour = 957, contour level = 0.25565 |
| 21055 | | |
| 21056 | | Position of COPI_cyto_J4022_model A (#27.1) relative to |
| 21057 | | W20_J4022_consensus_resampled.mrc (#8) coordinates: |
| 21058 | | Matrix rotation and translation |
| 21059 | | -0.39256776 -0.89898632 -0.19420133 280.18889645 |
| 21060 | | -0.78978108 0.22129853 0.57207763 162.30513777 |
| 21061 | | -0.47131349 0.37795577 -0.79687705 238.21825154 |
| 21062 | | Axis -0.54600989 0.77943814 0.30716346 |
| 21063 | | Axis point 209.81036508 0.00000000 69.55927246 |
| 21064 | | Rotation angle (degrees) 169.76045090 |
| 21065 | | Shift along axis 46.69284574 |
| 21066 | | |
| 21067 | | |
| 21068 | | > fitmap #27.2 inMap #8 |
| 21069 | | |
| 21070 | | Fit molecule COPI_cyto_J4022_model B (#27.2) to map |
| 21071 | | W20_J4022_consensus_resampled.mrc (#8) using 2426 atoms |
| 21072 | | average map value = 0.2446, steps = 52 |
| 21073 | | shifted from previous position = 1.11 |
| 21074 | | rotated from previous position = 2.79 degrees |
| 21075 | | atoms outside contour = 1336, contour level = 0.25565 |
| 21076 | | |
| 21077 | | Position of COPI_cyto_J4022_model B (#27.2) relative to |
| 21078 | | W20_J4022_consensus_resampled.mrc (#8) coordinates: |
| 21079 | | Matrix rotation and translation |
| 21080 | | -0.38976486 -0.89769297 -0.20550111 280.56757169 |
| 21081 | | -0.80102851 0.22038063 0.55658397 165.91242827 |
| 21082 | | -0.45435305 0.38154912 -0.80497428 236.80345949 |
| 21083 | | Axis -0.54830136 0.77953535 0.30280399 |
| 21084 | | Axis point 210.09627043 0.00000000 70.38992448 |
| 21085 | | Rotation angle (degrees) 170.81541733 |
| 21086 | | Shift along axis 47.20405452 |
| 21087 | | |
| 21088 | | |
| 21089 | | > fitmap #27.3 inMap #8 |
| 21090 | | |
| 21091 | | Fit molecule COPI_cyto_J4022_model C (#27.3) to map |
| 21092 | | W20_J4022_consensus_resampled.mrc (#8) using 1612 atoms |
| 21093 | | average map value = 0.2655, steps = 76 |
| 21094 | | shifted from previous position = 1.78 |
| 21095 | | rotated from previous position = 8.48 degrees |
| 21096 | | atoms outside contour = 633, contour level = 0.25565 |
| 21097 | | |
| 21098 | | Position of COPI_cyto_J4022_model C (#27.3) relative to |
| 21099 | | W20_J4022_consensus_resampled.mrc (#8) coordinates: |
| 21100 | | Matrix rotation and translation |
| 21101 | | -0.44384488 -0.89370270 -0.06555325 276.26301732 |
| 21102 | | -0.70850239 0.30519488 0.63630219 146.54842255 |
| 21103 | | -0.54865846 0.32886410 -0.76864967 245.76532786 |
| 21104 | | Axis -0.51083267 0.80271747 0.30772494 |
| 21105 | | Axis point 206.80155193 0.00000000 65.73198008 |
| 21106 | | Rotation angle (degrees) 162.48721631 |
| 21107 | | Shift along axis 52.14092594 |
| 21108 | | |
| 21109 | | |
| 21110 | | > fitmap #27.4 inMap #8 |
| 21111 | | |
| 21112 | | Fit molecule COPI_cyto_J4022_model D (#27.4) to map |
| 21113 | | W20_J4022_consensus_resampled.mrc (#8) using 4745 atoms |
| 21114 | | average map value = 0.3044, steps = 100 |
| 21115 | | shifted from previous position = 0.615 |
| 21116 | | rotated from previous position = 1.41 degrees |
| 21117 | | atoms outside contour = 1677, contour level = 0.25565 |
| 21118 | | |
| 21119 | | Position of COPI_cyto_J4022_model D (#27.4) relative to |
| 21120 | | W20_J4022_consensus_resampled.mrc (#8) coordinates: |
| 21121 | | Matrix rotation and translation |
| 21122 | | -0.37672030 -0.91435784 -0.14843034 275.26026826 |
| 21123 | | -0.79965882 0.24012360 0.55035120 163.64023290 |
| 21124 | | -0.46757631 0.32602210 -0.82163374 244.18290661 |
| 21125 | | Axis -0.55170270 0.78489010 0.28208449 |
| 21126 | | Axis point 210.28195260 0.00000000 73.03172771 |
| 21127 | | Rotation angle (degrees) 168.26963934 |
| 21128 | | Shift along axis 45.45797518 |
| 21129 | | |
| 21130 | | |
| 21131 | | > fitmap #27.5 inMap #8 |
| 21132 | | |
| 21133 | | Fit molecule COPI_cyto_J4022_model E (#27.5) to map |
| 21134 | | W20_J4022_consensus_resampled.mrc (#8) using 1876 atoms |
| 21135 | | average map value = 0.2625, steps = 52 |
| 21136 | | shifted from previous position = 0.552 |
| 21137 | | rotated from previous position = 1.61 degrees |
| 21138 | | atoms outside contour = 794, contour level = 0.25565 |
| 21139 | | |
| 21140 | | Position of COPI_cyto_J4022_model E (#27.5) relative to |
| 21141 | | W20_J4022_consensus_resampled.mrc (#8) coordinates: |
| 21142 | | Matrix rotation and translation |
| 21143 | | -0.39012142 -0.90485684 -0.17040949 278.02588034 |
| 21144 | | -0.78445694 0.22972243 0.57606852 161.46943730 |
| 21145 | | -0.48211265 0.35841557 -0.79944086 241.20120653 |
| 21146 | | Axis -0.54579308 0.78163622 0.30191841 |
| 21147 | | Axis point 210.00611855 0.00000000 69.84237754 |
| 21148 | | Rotation angle (degrees) 168.49862507 |
| 21149 | | Shift along axis 47.28884646 |
| 21150 | | |
| 21151 | | |
| 21152 | | > fitmap #27.6 inMap #8 |
| 21153 | | |
| 21154 | | Fit molecule COPI_cyto_J4022_model F (#27.6) to map |
| 21155 | | W20_J4022_consensus_resampled.mrc (#8) using 2400 atoms |
| 21156 | | average map value = 0.2992, steps = 68 |
| 21157 | | shifted from previous position = 0.833 |
| 21158 | | rotated from previous position = 1.23 degrees |
| 21159 | | atoms outside contour = 836, contour level = 0.25565 |
| 21160 | | |
| 21161 | | Position of COPI_cyto_J4022_model F (#27.6) relative to |
| 21162 | | W20_J4022_consensus_resampled.mrc (#8) coordinates: |
| 21163 | | Matrix rotation and translation |
| 21164 | | -0.37627915 -0.91501112 -0.14549456 274.06344417 |
| 21165 | | -0.78910526 0.23420927 0.56785467 161.94404988 |
| 21166 | | -0.48551716 0.32848239 -0.81016814 243.51929054 |
| 21167 | | Axis -0.55094864 0.78260937 0.28978988 |
| 21168 | | Axis point 210.19577001 0.00000000 70.73622595 |
| 21169 | | Rotation angle (degrees) 167.45323080 |
| 21170 | | Shift along axis 46.31347447 |
| 21171 | | |
| 21172 | | |
| 21173 | | > fitmap #27.7 inMap #8 |
| 21174 | | |
| 21175 | | Fit molecule COPI_cyto_J4022_model G (#27.7) to map |
| 21176 | | W20_J4022_consensus_resampled.mrc (#8) using 2302 atoms |
| 21177 | | average map value = 0.2826, steps = 96 |
| 21178 | | shifted from previous position = 2.92 |
| 21179 | | rotated from previous position = 2.79 degrees |
| 21180 | | atoms outside contour = 976, contour level = 0.25565 |
| 21181 | | |
| 21182 | | Position of COPI_cyto_J4022_model G (#27.7) relative to |
| 21183 | | W20_J4022_consensus_resampled.mrc (#8) coordinates: |
| 21184 | | Matrix rotation and translation |
| 21185 | | -0.33451130 -0.92639130 -0.17292012 274.85479282 |
| 21186 | | -0.79442923 0.17849847 0.58053467 167.37506321 |
| 21187 | | -0.50693629 0.33156820 -0.79566208 241.26568780 |
| 21188 | | Axis -0.56972315 0.76434688 0.30197578 |
| 21189 | | Axis point 216.29332055 0.00000000 65.86543953 |
| 21190 | | Rotation angle (degrees) 167.37918527 |
| 21191 | | Shift along axis 44.19786245 |
| 21192 | | |
| 21193 | | |
| 21194 | | > fitmap #27.8 inMap #8 |
| 21195 | | |
| 21196 | | Fit molecule COPI_cyto_J4022_model H (#27.8) to map |
| 21197 | | W20_J4022_consensus_resampled.mrc (#8) using 420 atoms |
| 21198 | | average map value = 0.3956, steps = 60 |
| 21199 | | shifted from previous position = 3.43 |
| 21200 | | rotated from previous position = 4.58 degrees |
| 21201 | | atoms outside contour = 107, contour level = 0.25565 |
| 21202 | | |
| 21203 | | Position of COPI_cyto_J4022_model H (#27.8) relative to |
| 21204 | | W20_J4022_consensus_resampled.mrc (#8) coordinates: |
| 21205 | | Matrix rotation and translation |
| 21206 | | -0.29409810 -0.94159516 -0.16402708 273.35842395 |
| 21207 | | -0.82182141 0.16150890 0.54637392 173.87777684 |
| 21208 | | -0.48797119 0.29548850 -0.82132252 245.93686122 |
| 21209 | | Axis -0.58771482 0.75885938 0.28057750 |
| 21210 | | Axis point 220.70161074 0.00000000 69.69363620 |
| 21211 | | Rotation angle (degrees) 167.67589275 |
| 21212 | | Shift along axis 40.29633569 |
| 21213 | | |
| 21214 | | |
| 21215 | | > fitmap #27.9 inMap #8 |
| 21216 | | |
| 21217 | | Fit molecule COPI_cyto_J4022_model I (#27.9) to map |
| 21218 | | W20_J4022_consensus_resampled.mrc (#8) using 1573 atoms |
| 21219 | | average map value = 0.2808, steps = 104 |
| 21220 | | shifted from previous position = 1.41 |
| 21221 | | rotated from previous position = 3.01 degrees |
| 21222 | | atoms outside contour = 658, contour level = 0.25565 |
| 21223 | | |
| 21224 | | Position of COPI_cyto_J4022_model I (#27.9) relative to |
| 21225 | | W20_J4022_consensus_resampled.mrc (#8) coordinates: |
| 21226 | | Matrix rotation and translation |
| 21227 | | -0.32433234 -0.92767253 -0.18501954 273.90297834 |
| 21228 | | -0.82148476 0.17924210 0.54132715 171.93921965 |
| 21229 | | -0.46901103 0.32756063 -0.82020284 242.13807924 |
| 21230 | | Axis -0.57622748 0.76552540 0.28623898 |
| 21231 | | Axis point 215.70475315 0.00000000 70.66930942 |
| 21232 | | Rotation angle (degrees) 169.31041717 |
| 21233 | | Shift along axis 43.10277500 |
| 21234 | | |
| 21235 | | |
| 21236 | | > fitmap #27.10 inMap #8 |
| 21237 | | |
| 21238 | | Fit molecule COPI_cyto_J4022_model J (#27.10) to map |
| 21239 | | W20_J4022_consensus_resampled.mrc (#8) using 1112 atoms |
| 21240 | | average map value = 0.33, steps = 88 |
| 21241 | | shifted from previous position = 0.662 |
| 21242 | | rotated from previous position = 3.39 degrees |
| 21243 | | atoms outside contour = 391, contour level = 0.25565 |
| 21244 | | |
| 21245 | | Position of COPI_cyto_J4022_model J (#27.10) relative to |
| 21246 | | W20_J4022_consensus_resampled.mrc (#8) coordinates: |
| 21247 | | Matrix rotation and translation |
| 21248 | | -0.38171857 -0.91794952 -0.10797977 270.82825471 |
| 21249 | | -0.78627841 0.26109129 0.55999787 160.73450027 |
| 21250 | | -0.48585720 0.29866375 -0.82142727 248.07112012 |
| 21251 | | Axis -0.54679573 0.79064209 0.27549866 |
| 21252 | | Axis point 209.01509765 0.00000000 73.28198391 |
| 21253 | | Rotation angle (degrees) 166.17432003 |
| 21254 | | Shift along axis 47.33898908 |
| 21255 | | |
| 21256 | | |
| 21257 | | > hide #!27 models |
| 21258 | | |
| 21259 | | > show #!28 models |
| 21260 | | |
| 21261 | | > split #28 chains |
| 21262 | | |
| 21263 | | Split COPI_cyto_J4023_model (#28) into 13 models |
| 21264 | | Chain information for COPI_cyto_J4023_model A #28.1 |
| 21265 | | --- |
| 21266 | | Chain | Description |
| 21267 | | A | No description available |
| 21268 | | |
| 21269 | | Chain information for COPI_cyto_J4023_model B #28.2 |
| 21270 | | --- |
| 21271 | | Chain | Description |
| 21272 | | B | No description available |
| 21273 | | |
| 21274 | | Chain information for COPI_cyto_J4023_model C #28.3 |
| 21275 | | --- |
| 21276 | | Chain | Description |
| 21277 | | C | No description available |
| 21278 | | |
| 21279 | | Chain information for COPI_cyto_J4023_model D #28.4 |
| 21280 | | --- |
| 21281 | | Chain | Description |
| 21282 | | D | No description available |
| 21283 | | |
| 21284 | | Chain information for COPI_cyto_J4023_model E #28.5 |
| 21285 | | --- |
| 21286 | | Chain | Description |
| 21287 | | E | No description available |
| 21288 | | |
| 21289 | | Chain information for COPI_cyto_J4023_model F #28.6 |
| 21290 | | --- |
| 21291 | | Chain | Description |
| 21292 | | F | No description available |
| 21293 | | |
| 21294 | | Chain information for COPI_cyto_J4023_model G #28.7 |
| 21295 | | --- |
| 21296 | | Chain | Description |
| 21297 | | G | No description available |
| 21298 | | |
| 21299 | | Chain information for COPI_cyto_J4023_model H #28.8 |
| 21300 | | --- |
| 21301 | | Chain | Description |
| 21302 | | H | No description available |
| 21303 | | |
| 21304 | | Chain information for COPI_cyto_J4023_model I #28.9 |
| 21305 | | --- |
| 21306 | | Chain | Description |
| 21307 | | I | No description available |
| 21308 | | |
| 21309 | | Chain information for COPI_cyto_J4023_model J #28.10 |
| 21310 | | --- |
| 21311 | | Chain | Description |
| 21312 | | J | No description available |
| 21313 | | |
| 21314 | | Chain information for COPI_cyto_J4023_model K #28.11 |
| 21315 | | --- |
| 21316 | | Chain | Description |
| 21317 | | K | No description available |
| 21318 | | |
| 21319 | | Chain information for COPI_cyto_J4023_model L #28.12 |
| 21320 | | --- |
| 21321 | | Chain | Description |
| 21322 | | L | No description available |
| 21323 | | |
| 21324 | | Chain information for COPI_cyto_J4023_model M #28.13 |
| 21325 | | --- |
| 21326 | | Chain | Description |
| 21327 | | M | No description available |
| 21328 | | |
| 21329 | | |
| 21330 | | > hide #!8 models |
| 21331 | | |
| 21332 | | > show #!10 models |
| 21333 | | |
| 21334 | | > color #10 darkgrey models |
| 21335 | | |
| 21336 | | > color #10 #a9a9a9c3 models |
| 21337 | | |
| 21338 | | > color #10 #a9a9a9c1 models |
| 21339 | | |
| 21340 | | > color #10 #a9a9a9c0 models |
| 21341 | | |
| 21342 | | > color #10 #a9a9a9bf models |
| 21343 | | |
| 21344 | | > fitmap #28.1 inMap #10 |
| 21345 | | |
| 21346 | | Fit molecule COPI_cyto_J4023_model A (#28.1) to map |
| 21347 | | W20_J4023_consensus_resampled.mrc (#10) using 2590 atoms |
| 21348 | | average map value = 0.2661, steps = 80 |
| 21349 | | shifted from previous position = 0.302 |
| 21350 | | rotated from previous position = 1.64 degrees |
| 21351 | | atoms outside contour = 989, contour level = 0.259 |
| 21352 | | |
| 21353 | | Position of COPI_cyto_J4023_model A (#28.1) relative to |
| 21354 | | W20_J4023_consensus_resampled.mrc (#10) coordinates: |
| 21355 | | Matrix rotation and translation |
| 21356 | | -0.38904601 -0.90521909 -0.17094331 278.16947599 |
| 21357 | | -0.78384179 0.22779200 0.57767021 160.52070200 |
| 21358 | | -0.48397858 0.35873280 -0.79817011 240.89357120 |
| 21359 | | Axis -0.54622301 0.78098606 0.30282204 |
| 21360 | | Axis point 209.92132129 0.00000000 69.65657344 |
| 21361 | | Rotation angle (degrees) 168.43904812 |
| 21362 | | Shift along axis 46.36974623 |
| 21363 | | |
| 21364 | | |
| 21365 | | > fitmap #28.2 inMap #10 |
| 21366 | | |
| 21367 | | Fit molecule COPI_cyto_J4023_model B (#28.2) to map |
| 21368 | | W20_J4023_consensus_resampled.mrc (#10) using 2426 atoms |
| 21369 | | average map value = 0.2462, steps = 52 |
| 21370 | | shifted from previous position = 0.807 |
| 21371 | | rotated from previous position = 1.71 degrees |
| 21372 | | atoms outside contour = 1372, contour level = 0.259 |
| 21373 | | |
| 21374 | | Position of COPI_cyto_J4023_model B (#28.2) relative to |
| 21375 | | W20_J4023_consensus_resampled.mrc (#10) coordinates: |
| 21376 | | Matrix rotation and translation |
| 21377 | | -0.37936173 -0.90522783 -0.19143475 278.45387337 |
| 21378 | | -0.80078335 0.21757935 0.55803698 165.73257662 |
| 21379 | | -0.46349835 0.36499563 -0.80743265 238.99424212 |
| 21380 | | Axis -0.55224090 0.77830294 0.29878840 |
| 21381 | | Axis point 210.75764690 0.00000000 70.47418382 |
| 21382 | | Rotation angle (degrees) 169.93414364 |
| 21383 | | Shift along axis 46.62524128 |
| 21384 | | |
| 21385 | | |
| 21386 | | > fitmap #28.3 inMap #10 |
| 21387 | | |
| 21388 | | Fit molecule COPI_cyto_J4023_model C (#28.3) to map |
| 21389 | | W20_J4023_consensus_resampled.mrc (#10) using 1612 atoms |
| 21390 | | average map value = 0.2674, steps = 100 |
| 21391 | | shifted from previous position = 1.78 |
| 21392 | | rotated from previous position = 13.1 degrees |
| 21393 | | atoms outside contour = 645, contour level = 0.259 |
| 21394 | | |
| 21395 | | Position of COPI_cyto_J4023_model C (#28.3) relative to |
| 21396 | | W20_J4023_consensus_resampled.mrc (#10) coordinates: |
| 21397 | | Matrix rotation and translation |
| 21398 | | -0.48320445 -0.87548576 -0.00617812 274.93344286 |
| 21399 | | -0.65530471 0.35698291 0.66568683 136.56063023 |
| 21400 | | -0.58059385 0.32571139 -0.74620566 247.99422947 |
| 21401 | | Axis -0.48370086 0.81725132 0.31326310 |
| 21402 | | Axis point 203.45931463 0.00000000 64.26982521 |
| 21403 | | Rotation angle (degrees) 159.42510249 |
| 21404 | | Shift along axis 56.30625377 |
| 21405 | | |
| 21406 | | |
| 21407 | | > fitmap #28.4 inMap #10 |
| 21408 | | |
| 21409 | | Fit molecule COPI_cyto_J4023_model D (#28.4) to map |
| 21410 | | W20_J4023_consensus_resampled.mrc (#10) using 4745 atoms |
| 21411 | | average map value = 0.2995, steps = 72 |
| 21412 | | shifted from previous position = 0.314 |
| 21413 | | rotated from previous position = 1.26 degrees |
| 21414 | | atoms outside contour = 1741, contour level = 0.259 |
| 21415 | | |
| 21416 | | Position of COPI_cyto_J4023_model D (#28.4) relative to |
| 21417 | | W20_J4023_consensus_resampled.mrc (#10) coordinates: |
| 21418 | | Matrix rotation and translation |
| 21419 | | -0.37631952 -0.91385434 -0.15249221 275.60221900 |
| 21420 | | -0.80067150 0.23796533 0.54981604 164.18546643 |
| 21421 | | -0.46616391 0.32900267 -0.82124812 243.17403703 |
| 21422 | | Axis -0.55210445 0.78428015 0.28299352 |
| 21423 | | Axis point 210.42278812 0.00000000 72.60304045 |
| 21424 | | Rotation angle (degrees) 168.46454551 |
| 21425 | | Shift along axis 45.42286901 |
| 21426 | | |
| 21427 | | |
| 21428 | | > fitmap #28.5 inMap #10 |
| 21429 | | |
| 21430 | | Fit molecule COPI_cyto_J4023_model E (#28.5) to map |
| 21431 | | W20_J4023_consensus_resampled.mrc (#10) using 1876 atoms |
| 21432 | | average map value = 0.2617, steps = 64 |
| 21433 | | shifted from previous position = 0.422 |
| 21434 | | rotated from previous position = 1.48 degrees |
| 21435 | | atoms outside contour = 839, contour level = 0.259 |
| 21436 | | |
| 21437 | | Position of COPI_cyto_J4023_model E (#28.5) relative to |
| 21438 | | W20_J4023_consensus_resampled.mrc (#10) coordinates: |
| 21439 | | Matrix rotation and translation |
| 21440 | | -0.38829706 -0.90758205 -0.15975054 277.05830941 |
| 21441 | | -0.78302109 0.23353287 0.57648971 160.99119705 |
| 21442 | | -0.48590471 0.34893730 -0.80133600 242.27299446 |
| 21443 | | Axis -0.54602993 0.78263260 0.29889383 |
| 21444 | | Axis point 210.04637843 0.00000000 70.01691196 |
| 21445 | | Rotation angle (degrees) 167.97315842 |
| 21446 | | Shift along axis 47.12873327 |
| 21447 | | |
| 21448 | | |
| 21449 | | > fitmap #28.6 inMap #10 |
| 21450 | | |
| 21451 | | Fit molecule COPI_cyto_J4023_model F (#28.6) to map |
| 21452 | | W20_J4023_consensus_resampled.mrc (#10) using 2400 atoms |
| 21453 | | average map value = 0.3111, steps = 56 |
| 21454 | | shifted from previous position = 2.06 |
| 21455 | | rotated from previous position = 4.34 degrees |
| 21456 | | atoms outside contour = 741, contour level = 0.259 |
| 21457 | | |
| 21458 | | Position of COPI_cyto_J4023_model F (#28.6) relative to |
| 21459 | | W20_J4023_consensus_resampled.mrc (#10) coordinates: |
| 21460 | | Matrix rotation and translation |
| 21461 | | -0.35666068 -0.90337062 -0.23814849 281.82055176 |
| 21462 | | -0.82036020 0.18087540 0.54248801 172.36641935 |
| 21463 | | -0.44699252 0.38885168 -0.80560044 235.22166541 |
| 21464 | | Axis -0.56435649 0.76715244 0.30492440 |
| 21465 | | Axis point 214.18344574 0.00000000 69.66627251 |
| 21466 | | Rotation angle (degrees) 172.17681998 |
| 21467 | | Shift along axis 44.90888683 |
| 21468 | | |
| 21469 | | |
| 21470 | | > fitmap #28.7 inMap #10 |
| 21471 | | |
| 21472 | | Fit molecule COPI_cyto_J4023_model G (#28.7) to map |
| 21473 | | W20_J4023_consensus_resampled.mrc (#10) using 2302 atoms |
| 21474 | | average map value = 0.2755, steps = 64 |
| 21475 | | shifted from previous position = 1.24 |
| 21476 | | rotated from previous position = 2.83 degrees |
| 21477 | | atoms outside contour = 963, contour level = 0.259 |
| 21478 | | |
| 21479 | | Position of COPI_cyto_J4023_model G (#28.7) relative to |
| 21480 | | W20_J4023_consensus_resampled.mrc (#10) coordinates: |
| 21481 | | Matrix rotation and translation |
| 21482 | | -0.37482050 -0.90249978 -0.21214088 278.18486892 |
| 21483 | | -0.80142293 0.20037819 0.56353340 166.38017125 |
| 21484 | | -0.46608036 0.38123844 -0.79838986 235.86468916 |
| 21485 | | Axis -0.55487743 0.77295215 0.30766217 |
| 21486 | | Axis point 210.65854364 0.00000000 68.54942126 |
| 21487 | | Rotation angle (degrees) 170.54540398 |
| 21488 | | Shift along axis 46.81204878 |
| 21489 | | |
| 21490 | | |
| 21491 | | > fitmap #28.8 inMap #10 |
| 21492 | | |
| 21493 | | Fit molecule COPI_cyto_J4023_model H (#28.8) to map |
| 21494 | | W20_J4023_consensus_resampled.mrc (#10) using 420 atoms |
| 21495 | | average map value = 0.3956, steps = 56 |
| 21496 | | shifted from previous position = 0.904 |
| 21497 | | rotated from previous position = 5.89 degrees |
| 21498 | | atoms outside contour = 91, contour level = 0.259 |
| 21499 | | |
| 21500 | | Position of COPI_cyto_J4023_model H (#28.8) relative to |
| 21501 | | W20_J4023_consensus_resampled.mrc (#10) coordinates: |
| 21502 | | Matrix rotation and translation |
| 21503 | | -0.28058215 -0.93606717 -0.21225441 275.82091929 |
| 21504 | | -0.83301532 0.12762624 0.53832800 180.46362086 |
| 21505 | | -0.47682192 0.32785640 -0.81556793 244.72635677 |
| 21506 | | Axis -0.59550860 0.74856765 0.29157501 |
| 21507 | | Axis point 223.51865643 0.00000000 69.31481173 |
| 21508 | | Rotation angle (degrees) 169.82147556 |
| 21509 | | Shift along axis 42.19158968 |
| 21510 | | |
| 21511 | | |
| 21512 | | > fitmap #28.9 inMap #10 |
| 21513 | | |
| 21514 | | Fit molecule COPI_cyto_J4023_model I (#28.9) to map |
| 21515 | | W20_J4023_consensus_resampled.mrc (#10) using 1573 atoms |
| 21516 | | average map value = 0.285, steps = 68 |
| 21517 | | shifted from previous position = 1.1 |
| 21518 | | rotated from previous position = 2.83 degrees |
| 21519 | | atoms outside contour = 662, contour level = 0.259 |
| 21520 | | |
| 21521 | | Position of COPI_cyto_J4023_model I (#28.9) relative to |
| 21522 | | W20_J4023_consensus_resampled.mrc (#10) coordinates: |
| 21523 | | Matrix rotation and translation |
| 21524 | | -0.34800803 -0.91546944 -0.20200525 275.41256013 |
| 21525 | | -0.79922182 0.17708111 0.57435771 168.85645411 |
| 21526 | | -0.49003561 0.36132810 -0.79328880 241.32530949 |
| 21527 | | Axis -0.56560752 0.76473942 0.30864502 |
| 21528 | | Axis point 214.22374441 0.00000000 67.79866361 |
| 21529 | | Rotation angle (degrees) 169.14528401 |
| 21530 | | Shift along axis 47.83962632 |
| 21531 | | |
| 21532 | | |
| 21533 | | > fitmap #28.10 inMap #10 |
| 21534 | | |
| 21535 | | Fit molecule COPI_cyto_J4023_model J (#28.10) to map |
| 21536 | | W20_J4023_consensus_resampled.mrc (#10) using 1112 atoms |
| 21537 | | average map value = 0.3207, steps = 84 |
| 21538 | | shifted from previous position = 0.351 |
| 21539 | | rotated from previous position = 3.94 degrees |
| 21540 | | atoms outside contour = 429, contour level = 0.259 |
| 21541 | | |
| 21542 | | Position of COPI_cyto_J4023_model J (#28.10) relative to |
| 21543 | | W20_J4023_consensus_resampled.mrc (#10) coordinates: |
| 21544 | | Matrix rotation and translation |
| 21545 | | -0.37572939 -0.92009445 -0.11069619 271.04311764 |
| 21546 | | -0.80434258 0.26444766 0.53207186 165.17448016 |
| 21547 | | -0.46028301 0.28895269 -0.83943190 247.79248250 |
| 21548 | | Axis -0.55095733 0.79223463 0.26231720 |
| 21549 | | Axis point 209.56379921 0.00000000 75.63736830 |
| 21550 | | Rotation angle (degrees) 167.25376251 |
| 21551 | | Shift along axis 46.52397993 |
| 21552 | | |
| 21553 | | |
| 21554 | | > hide #!10 models |
| 21555 | | |
| 21556 | | > show #!12 models |
| 21557 | | |
| 21558 | | > color #12 darkgrey models |
| 21559 | | |
| 21560 | | > color #12 #a9a9a9c0 models |
| 21561 | | |
| 21562 | | > color #12 #a9a9a9bf models |
| 21563 | | |
| 21564 | | > hide #!28 models |
| 21565 | | |
| 21566 | | > show #!29 models |
| 21567 | | |
| 21568 | | > split #29 chains |
| 21569 | | |
| 21570 | | Split COPI_cyto_J4024_model (#29) into 13 models |
| 21571 | | Chain information for COPI_cyto_J4024_model A #29.1 |
| 21572 | | --- |
| 21573 | | Chain | Description |
| 21574 | | A | No description available |
| 21575 | | |
| 21576 | | Chain information for COPI_cyto_J4024_model B #29.2 |
| 21577 | | --- |
| 21578 | | Chain | Description |
| 21579 | | B | No description available |
| 21580 | | |
| 21581 | | Chain information for COPI_cyto_J4024_model C #29.3 |
| 21582 | | --- |
| 21583 | | Chain | Description |
| 21584 | | C | No description available |
| 21585 | | |
| 21586 | | Chain information for COPI_cyto_J4024_model D #29.4 |
| 21587 | | --- |
| 21588 | | Chain | Description |
| 21589 | | D | No description available |
| 21590 | | |
| 21591 | | Chain information for COPI_cyto_J4024_model E #29.5 |
| 21592 | | --- |
| 21593 | | Chain | Description |
| 21594 | | E | No description available |
| 21595 | | |
| 21596 | | Chain information for COPI_cyto_J4024_model F #29.6 |
| 21597 | | --- |
| 21598 | | Chain | Description |
| 21599 | | F | No description available |
| 21600 | | |
| 21601 | | Chain information for COPI_cyto_J4024_model G #29.7 |
| 21602 | | --- |
| 21603 | | Chain | Description |
| 21604 | | G | No description available |
| 21605 | | |
| 21606 | | Chain information for COPI_cyto_J4024_model H #29.8 |
| 21607 | | --- |
| 21608 | | Chain | Description |
| 21609 | | H | No description available |
| 21610 | | |
| 21611 | | Chain information for COPI_cyto_J4024_model I #29.9 |
| 21612 | | --- |
| 21613 | | Chain | Description |
| 21614 | | I | No description available |
| 21615 | | |
| 21616 | | Chain information for COPI_cyto_J4024_model J #29.10 |
| 21617 | | --- |
| 21618 | | Chain | Description |
| 21619 | | J | No description available |
| 21620 | | |
| 21621 | | Chain information for COPI_cyto_J4024_model K #29.11 |
| 21622 | | --- |
| 21623 | | Chain | Description |
| 21624 | | K | No description available |
| 21625 | | |
| 21626 | | Chain information for COPI_cyto_J4024_model L #29.12 |
| 21627 | | --- |
| 21628 | | Chain | Description |
| 21629 | | L | No description available |
| 21630 | | |
| 21631 | | Chain information for COPI_cyto_J4024_model M #29.13 |
| 21632 | | --- |
| 21633 | | Chain | Description |
| 21634 | | M | No description available |
| 21635 | | |
| 21636 | | |
| 21637 | | > fitmap #29.1 inMap #12 |
| 21638 | | |
| 21639 | | Fit molecule COPI_cyto_J4024_model A (#29.1) to map |
| 21640 | | W20_J4024_consensus_resampled.mrc (#12) using 2590 atoms |
| 21641 | | average map value = 0.2692, steps = 100 |
| 21642 | | shifted from previous position = 0.874 |
| 21643 | | rotated from previous position = 1.25 degrees |
| 21644 | | atoms outside contour = 892, contour level = 0.25569 |
| 21645 | | |
| 21646 | | Position of COPI_cyto_J4024_model A (#29.1) relative to |
| 21647 | | W20_J4024_consensus_resampled.mrc (#12) coordinates: |
| 21648 | | Matrix rotation and translation |
| 21649 | | -0.38195228 -0.90644822 -0.18017798 277.95847443 |
| 21650 | | -0.79414648 0.22219499 0.56565075 163.75119780 |
| 21651 | | -0.47269847 0.35913930 -0.80472054 240.33850306 |
| 21652 | | Axis -0.55030232 0.77949527 0.29925655 |
| 21653 | | Axis point 210.52708604 0.00000000 70.30220517 |
| 21654 | | Rotation angle (degrees) 169.18522876 |
| 21655 | | Shift along axis 46.60496312 |
| 21656 | | |
| 21657 | | |
| 21658 | | > fitmap #29.2 inMap #12 |
| 21659 | | |
| 21660 | | Fit molecule COPI_cyto_J4024_model B (#29.2) to map |
| 21661 | | W20_J4024_consensus_resampled.mrc (#12) using 2426 atoms |
| 21662 | | average map value = 0.2485, steps = 64 |
| 21663 | | shifted from previous position = 0.888 |
| 21664 | | rotated from previous position = 2.24 degrees |
| 21665 | | atoms outside contour = 1271, contour level = 0.25569 |
| 21666 | | |
| 21667 | | Position of COPI_cyto_J4024_model B (#29.2) relative to |
| 21668 | | W20_J4024_consensus_resampled.mrc (#12) coordinates: |
| 21669 | | Matrix rotation and translation |
| 21670 | | -0.37729450 -0.90386908 -0.20166693 279.03898781 |
| 21671 | | -0.80059309 0.20887819 0.56162321 165.93012621 |
| 21672 | | -0.46551002 0.37335050 -0.80243993 238.54192108 |
| 21673 | | Axis -0.55344239 0.77558746 0.30358789 |
| 21674 | | Axis point 211.11340356 0.00000000 69.95800816 |
| 21675 | | Rotation angle (degrees) 170.20680766 |
| 21676 | | Shift along axis 46.67975878 |
| 21677 | | |
| 21678 | | |
| 21679 | | > fitmap #29.3 inMap #12 |
| 21680 | | |
| 21681 | | Fit molecule COPI_cyto_J4024_model C (#29.3) to map |
| 21682 | | W20_J4024_consensus_resampled.mrc (#12) using 1612 atoms |
| 21683 | | average map value = 0.2695, steps = 160 |
| 21684 | | shifted from previous position = 2.41 |
| 21685 | | rotated from previous position = 18.3 degrees |
| 21686 | | atoms outside contour = 621, contour level = 0.25569 |
| 21687 | | |
| 21688 | | Position of COPI_cyto_J4024_model C (#29.3) relative to |
| 21689 | | W20_J4024_consensus_resampled.mrc (#12) coordinates: |
| 21690 | | Matrix rotation and translation |
| 21691 | | -0.48164802 -0.86999176 0.10549661 268.61459966 |
| 21692 | | -0.62073770 0.42365137 0.65970011 129.62131912 |
| 21693 | | -0.61862744 0.25225752 -0.74408753 255.46187775 |
| 21694 | | Axis -0.46969429 0.83476038 0.28733670 |
| 21695 | | Axis point 203.50844953 0.00000000 65.15506927 |
| 21696 | | Rotation angle (degrees) 154.29538375 |
| 21697 | | Shift along axis 55.43957254 |
| 21698 | | |
| 21699 | | |
| 21700 | | > fitmap #29.4 inMap #12 |
| 21701 | | |
| 21702 | | Fit molecule COPI_cyto_J4024_model D (#29.4) to map |
| 21703 | | W20_J4024_consensus_resampled.mrc (#12) using 4745 atoms |
| 21704 | | average map value = 0.311, steps = 96 |
| 21705 | | shifted from previous position = 0.393 |
| 21706 | | rotated from previous position = 1.84 degrees |
| 21707 | | atoms outside contour = 1582, contour level = 0.25569 |
| 21708 | | |
| 21709 | | Position of COPI_cyto_J4024_model D (#29.4) relative to |
| 21710 | | W20_J4024_consensus_resampled.mrc (#12) coordinates: |
| 21711 | | Matrix rotation and translation |
| 21712 | | -0.36490275 -0.91923092 -0.14785303 274.26364105 |
| 21713 | | -0.80817834 0.23388135 0.54050653 165.94975145 |
| 21714 | | -0.46227025 0.31672393 -0.82824645 244.92475483 |
| 21715 | | Axis -0.55725013 0.78294306 0.27653654 |
| 21716 | | Axis point 211.24999279 0.00000000 73.77234724 |
| 21717 | | Rotation angle (degrees) 168.41673025 |
| 21718 | | Shift along axis 44.82640132 |
| 21719 | | |
| 21720 | | |
| 21721 | | > fitmap #29.5 inMap #12 |
| 21722 | | |
| 21723 | | Fit molecule COPI_cyto_J4024_model E (#29.5) to map |
| 21724 | | W20_J4024_consensus_resampled.mrc (#12) using 1876 atoms |
| 21725 | | average map value = 0.2646, steps = 56 |
| 21726 | | shifted from previous position = 0.686 |
| 21727 | | rotated from previous position = 1.54 degrees |
| 21728 | | atoms outside contour = 791, contour level = 0.25569 |
| 21729 | | |
| 21730 | | Position of COPI_cyto_J4024_model E (#29.5) relative to |
| 21731 | | W20_J4024_consensus_resampled.mrc (#12) coordinates: |
| 21732 | | Matrix rotation and translation |
| 21733 | | -0.38725918 -0.90565201 -0.17269853 278.00845664 |
| 21734 | | -0.78536225 0.22592118 0.57633824 161.81751759 |
| 21735 | | -0.48294563 0.35882318 -0.79875495 241.37260249 |
| 21736 | | Axis -0.54715674 0.78042314 0.30258755 |
| 21737 | | Axis point 210.33473059 0.00000000 69.77477808 |
| 21738 | | Rotation angle (degrees) 168.53504549 |
| 21739 | | Shift along axis 47.20827915 |
| 21740 | | |
| 21741 | | |
| 21742 | | > fitmap #29.6 inMap #12 |
| 21743 | | |
| 21744 | | Fit molecule COPI_cyto_J4024_model F (#29.6) to map |
| 21745 | | W20_J4024_consensus_resampled.mrc (#12) using 2400 atoms |
| 21746 | | average map value = 0.242, steps = 84 |
| 21747 | | shifted from previous position = 5.87 |
| 21748 | | rotated from previous position = 13.7 degrees |
| 21749 | | atoms outside contour = 1297, contour level = 0.25569 |
| 21750 | | |
| 21751 | | Position of COPI_cyto_J4024_model F (#29.6) relative to |
| 21752 | | W20_J4024_consensus_resampled.mrc (#12) coordinates: |
| 21753 | | Matrix rotation and translation |
| 21754 | | -0.53159589 -0.84531226 -0.05341354 266.88246547 |
| 21755 | | -0.64371973 0.36222400 0.67410585 127.52327824 |
| 21756 | | -0.55048227 0.39273525 -0.73670097 230.79457007 |
| 21757 | | Axis -0.46452916 0.82063626 0.33281944 |
| 21758 | | Axis point 189.53211141 0.00000000 61.79948623 |
| 21759 | | Rotation angle (degrees) 162.37079623 |
| 21760 | | Shift along axis 57.48845832 |
| 21761 | | |
| 21762 | | |
| 21763 | | > fitmap #29.7 inMap #12 |
| 21764 | | |
| 21765 | | Fit molecule COPI_cyto_J4024_model G (#29.7) to map |
| 21766 | | W20_J4024_consensus_resampled.mrc (#12) using 2302 atoms |
| 21767 | | average map value = 0.2775, steps = 60 |
| 21768 | | shifted from previous position = 3.1 |
| 21769 | | rotated from previous position = 4.14 degrees |
| 21770 | | atoms outside contour = 948, contour level = 0.25569 |
| 21771 | | |
| 21772 | | Position of COPI_cyto_J4024_model G (#29.7) relative to |
| 21773 | | W20_J4024_consensus_resampled.mrc (#12) coordinates: |
| 21774 | | Matrix rotation and translation |
| 21775 | | -0.36698963 -0.92232957 -0.12094124 274.70465819 |
| 21776 | | -0.76065109 0.22270273 0.60976506 158.64044193 |
| 21777 | | -0.53547040 0.31577154 -0.78330058 243.71016364 |
| 21778 | | Axis -0.55127458 0.77729395 0.30316736 |
| 21779 | | Axis point 213.68280008 0.00000000 64.72109245 |
| 21780 | | Rotation angle (degrees) 164.53504616 |
| 21781 | | Shift along axis 45.75752709 |
| 21782 | | |
| 21783 | | |
| 21784 | | > fitmap #29.8 inMap #12 |
| 21785 | | |
| 21786 | | Fit molecule COPI_cyto_J4024_model H (#29.8) to map |
| 21787 | | W20_J4024_consensus_resampled.mrc (#12) using 420 atoms |
| 21788 | | average map value = 0.3642, steps = 52 |
| 21789 | | shifted from previous position = 3.12 |
| 21790 | | rotated from previous position = 7.89 degrees |
| 21791 | | atoms outside contour = 112, contour level = 0.25569 |
| 21792 | | |
| 21793 | | Position of COPI_cyto_J4024_model H (#29.8) relative to |
| 21794 | | W20_J4024_consensus_resampled.mrc (#12) coordinates: |
| 21795 | | Matrix rotation and translation |
| 21796 | | -0.31100107 -0.94914560 -0.04899985 269.58279474 |
| 21797 | | -0.77806832 0.22466013 0.58663236 161.32624746 |
| 21798 | | -0.54579120 0.22056852 -0.80836966 258.53074932 |
| 21799 | | Axis -0.57164561 0.77578980 0.26715442 |
| 21800 | | Axis point 220.48916877 0.00000000 70.49015049 |
| 21801 | | Rotation angle (degrees) 161.32593230 |
| 21802 | | Shift along axis 40.11707004 |
| 21803 | | |
| 21804 | | |
| 21805 | | > fitmap #29.9 inMap #12 |
| 21806 | | |
| 21807 | | Fit molecule COPI_cyto_J4024_model I (#29.9) to map |
| 21808 | | W20_J4024_consensus_resampled.mrc (#12) using 1573 atoms |
| 21809 | | average map value = 0.308, steps = 88 |
| 21810 | | shifted from previous position = 1.06 |
| 21811 | | rotated from previous position = 3.68 degrees |
| 21812 | | atoms outside contour = 615, contour level = 0.25569 |
| 21813 | | |
| 21814 | | Position of COPI_cyto_J4024_model I (#29.9) relative to |
| 21815 | | W20_J4024_consensus_resampled.mrc (#12) coordinates: |
| 21816 | | Matrix rotation and translation |
| 21817 | | -0.32207758 -0.93636157 -0.13961755 272.41905490 |
| 21818 | | -0.79316925 0.18637492 0.57978181 166.78930021 |
| 21819 | | -0.51686418 0.29747507 -0.80272038 248.24026118 |
| 21820 | | Axis -0.57325903 0.76604633 0.29076985 |
| 21821 | | Axis point 217.83744421 0.00000000 68.18432836 |
| 21822 | | Rotation angle (degrees) 165.74548655 |
| 21823 | | Shift along axis 43.78243158 |
| 21824 | | |
| 21825 | | |
| 21826 | | > fitmap #29.10 inMap #12 |
| 21827 | | |
| 21828 | | Fit molecule COPI_cyto_J4024_model J (#29.10) to map |
| 21829 | | W20_J4024_consensus_resampled.mrc (#12) using 1112 atoms |
| 21830 | | average map value = 0.3363, steps = 72 |
| 21831 | | shifted from previous position = 0.618 |
| 21832 | | rotated from previous position = 4.66 degrees |
| 21833 | | atoms outside contour = 406, contour level = 0.25569 |
| 21834 | | |
| 21835 | | Position of COPI_cyto_J4024_model J (#29.10) relative to |
| 21836 | | W20_J4024_consensus_resampled.mrc (#12) coordinates: |
| 21837 | | Matrix rotation and translation |
| 21838 | | -0.36498462 -0.92690394 -0.08738035 267.73871712 |
| 21839 | | -0.79378094 0.26076630 0.54946590 162.75689323 |
| 21840 | | -0.48651625 0.26990746 -0.83093436 251.13764208 |
| 21841 | | Axis -0.55340845 0.79012166 0.26352770 |
| 21842 | | Axis point 210.27082462 0.00000000 74.58174748 |
| 21843 | | Rotation angle (degrees) 165.36983736 |
| 21844 | | Shift along axis 46.61060410 |
| 21845 | | |
| 21846 | | |
| 21847 | | > hide #!12 models |
| 21848 | | |
| 21849 | | > hide #!29 models |
| 21850 | | |
| 21851 | | > show #!30 models |
| 21852 | | |
| 21853 | | > split #30 chains |
| 21854 | | |
| 21855 | | Split COPI_cyto_J4025_model (#30) into 13 models |
| 21856 | | Chain information for COPI_cyto_J4025_model A #30.1 |
| 21857 | | --- |
| 21858 | | Chain | Description |
| 21859 | | A | No description available |
| 21860 | | |
| 21861 | | Chain information for COPI_cyto_J4025_model B #30.2 |
| 21862 | | --- |
| 21863 | | Chain | Description |
| 21864 | | B | No description available |
| 21865 | | |
| 21866 | | Chain information for COPI_cyto_J4025_model C #30.3 |
| 21867 | | --- |
| 21868 | | Chain | Description |
| 21869 | | C | No description available |
| 21870 | | |
| 21871 | | Chain information for COPI_cyto_J4025_model D #30.4 |
| 21872 | | --- |
| 21873 | | Chain | Description |
| 21874 | | D | No description available |
| 21875 | | |
| 21876 | | Chain information for COPI_cyto_J4025_model E #30.5 |
| 21877 | | --- |
| 21878 | | Chain | Description |
| 21879 | | E | No description available |
| 21880 | | |
| 21881 | | Chain information for COPI_cyto_J4025_model F #30.6 |
| 21882 | | --- |
| 21883 | | Chain | Description |
| 21884 | | F | No description available |
| 21885 | | |
| 21886 | | Chain information for COPI_cyto_J4025_model G #30.7 |
| 21887 | | --- |
| 21888 | | Chain | Description |
| 21889 | | G | No description available |
| 21890 | | |
| 21891 | | Chain information for COPI_cyto_J4025_model H #30.8 |
| 21892 | | --- |
| 21893 | | Chain | Description |
| 21894 | | H | No description available |
| 21895 | | |
| 21896 | | Chain information for COPI_cyto_J4025_model I #30.9 |
| 21897 | | --- |
| 21898 | | Chain | Description |
| 21899 | | I | No description available |
| 21900 | | |
| 21901 | | Chain information for COPI_cyto_J4025_model J #30.10 |
| 21902 | | --- |
| 21903 | | Chain | Description |
| 21904 | | J | No description available |
| 21905 | | |
| 21906 | | Chain information for COPI_cyto_J4025_model K #30.11 |
| 21907 | | --- |
| 21908 | | Chain | Description |
| 21909 | | K | No description available |
| 21910 | | |
| 21911 | | Chain information for COPI_cyto_J4025_model L #30.12 |
| 21912 | | --- |
| 21913 | | Chain | Description |
| 21914 | | L | No description available |
| 21915 | | |
| 21916 | | Chain information for COPI_cyto_J4025_model M #30.13 |
| 21917 | | --- |
| 21918 | | Chain | Description |
| 21919 | | M | No description available |
| 21920 | | |
| 21921 | | |
| 21922 | | > show #!14 models |
| 21923 | | |
| 21924 | | > color #14 darkgrey models |
| 21925 | | |
| 21926 | | > color #14 #a9a9a9be models |
| 21927 | | |
| 21928 | | > color #14 #a9a9a9bf models |
| 21929 | | |
| 21930 | | > fitmap #30.1 inMap #14 |
| 21931 | | |
| 21932 | | Fit molecule COPI_cyto_J4025_model A (#30.1) to map |
| 21933 | | W20_J4025_consensus_resampled.mrc (#14) using 2590 atoms |
| 21934 | | average map value = 0.2675, steps = 76 |
| 21935 | | shifted from previous position = 0.303 |
| 21936 | | rotated from previous position = 0.916 degrees |
| 21937 | | atoms outside contour = 971, contour level = 0.25978 |
| 21938 | | |
| 21939 | | Position of COPI_cyto_J4025_model A (#30.1) relative to |
| 21940 | | W20_J4025_consensus_resampled.mrc (#14) coordinates: |
| 21941 | | Matrix rotation and translation |
| 21942 | | -0.38101620 -0.91006695 -0.16310984 276.49091025 |
| 21943 | | -0.78874603 0.22789915 0.57091304 162.02364661 |
| 21944 | | -0.48239649 0.34617935 -0.80464495 242.12831797 |
| 21945 | | Axis -0.54965686 0.78091585 0.29672844 |
| 21946 | | Axis point 210.37135033 0.00000000 70.30651243 |
| 21947 | | Rotation angle (degrees) 168.20381385 |
| 21948 | | Shift along axis 46.39806523 |
| 21949 | | |
| 21950 | | |
| 21951 | | > fitmap #30.2 inMap #14 |
| 21952 | | |
| 21953 | | Fit molecule COPI_cyto_J4025_model B (#30.2) to map |
| 21954 | | W20_J4025_consensus_resampled.mrc (#14) using 2426 atoms |
| 21955 | | average map value = 0.2464, steps = 56 |
| 21956 | | shifted from previous position = 0.713 |
| 21957 | | rotated from previous position = 1.44 degrees |
| 21958 | | atoms outside contour = 1409, contour level = 0.25978 |
| 21959 | | |
| 21960 | | Position of COPI_cyto_J4025_model B (#30.2) relative to |
| 21961 | | W20_J4025_consensus_resampled.mrc (#14) coordinates: |
| 21962 | | Matrix rotation and translation |
| 21963 | | -0.37124088 -0.90903393 -0.18930807 277.53484582 |
| 21964 | | -0.80004519 0.20967074 0.56210843 165.71657123 |
| 21965 | | -0.47128327 0.36013264 -0.80510656 240.05521807 |
| 21966 | | Axis -0.55553559 0.77557441 0.29977415 |
| 21967 | | Axis point 211.48229973 0.00000000 70.08195116 |
| 21968 | | Rotation angle (degrees) 169.52626651 |
| 21969 | | Shift along axis 46.30739501 |
| 21970 | | |
| 21971 | | |
| 21972 | | > fitmap #30.3 inMap #14 |
| 21973 | | |
| 21974 | | Fit molecule COPI_cyto_J4025_model C (#30.3) to map |
| 21975 | | W20_J4025_consensus_resampled.mrc (#14) using 1612 atoms |
| 21976 | | average map value = 0.2632, steps = 96 |
| 21977 | | shifted from previous position = 1.92 |
| 21978 | | rotated from previous position = 10.7 degrees |
| 21979 | | atoms outside contour = 694, contour level = 0.25978 |
| 21980 | | |
| 21981 | | Position of COPI_cyto_J4025_model C (#30.3) relative to |
| 21982 | | W20_J4025_consensus_resampled.mrc (#14) coordinates: |
| 21983 | | Matrix rotation and translation |
| 21984 | | -0.44316515 -0.89625169 -0.01837289 273.18677871 |
| 21985 | | -0.69089200 0.32841899 0.64405685 142.84601321 |
| 21986 | | -0.57120303 0.29811723 -0.76475697 249.76514069 |
| 21987 | | Axis -0.50596953 0.80856656 0.30035804 |
| 21988 | | Axis point 206.79517933 0.00000000 65.69504944 |
| 21989 | | Rotation angle (degrees) 160.00987675 |
| 21990 | | Shift along axis 52.29529270 |
| 21991 | | |
| 21992 | | |
| 21993 | | > fitmap #30.4 inMap #14 |
| 21994 | | |
| 21995 | | Fit molecule COPI_cyto_J4025_model D (#30.4) to map |
| 21996 | | W20_J4025_consensus_resampled.mrc (#14) using 4745 atoms |
| 21997 | | average map value = 0.2994, steps = 96 |
| 21998 | | shifted from previous position = 0.519 |
| 21999 | | rotated from previous position = 1.3 degrees |
| 22000 | | atoms outside contour = 1727, contour level = 0.25978 |
| 22001 | | |
| 22002 | | Position of COPI_cyto_J4025_model D (#30.4) relative to |
| 22003 | | W20_J4025_consensus_resampled.mrc (#14) coordinates: |
| 22004 | | Matrix rotation and translation |
| 22005 | | -0.37140408 -0.91616900 -0.15064322 274.98034054 |
| 22006 | | -0.80234855 0.23505413 0.54862225 164.28861064 |
| 22007 | | -0.46722138 0.32462891 -0.82238693 243.65233050 |
| 22008 | | Axis -0.55421176 0.78328822 0.28161834 |
| 22009 | | Axis point 210.66577055 0.00000000 72.75003830 |
| 22010 | | Rotation angle (degrees) 168.34121758 |
| 22011 | | Shift along axis 44.90495816 |
| 22012 | | |
| 22013 | | |
| 22014 | | > fitmap #30.5 inMap #14 |
| 22015 | | |
| 22016 | | Fit molecule COPI_cyto_J4025_model E (#30.5) to map |
| 22017 | | W20_J4025_consensus_resampled.mrc (#14) using 1876 atoms |
| 22018 | | average map value = 0.2637, steps = 64 |
| 22019 | | shifted from previous position = 0.305 |
| 22020 | | rotated from previous position = 1.58 degrees |
| 22021 | | atoms outside contour = 846, contour level = 0.25978 |
| 22022 | | |
| 22023 | | Position of COPI_cyto_J4025_model E (#30.5) relative to |
| 22024 | | W20_J4025_consensus_resampled.mrc (#14) coordinates: |
| 22025 | | Matrix rotation and translation |
| 22026 | | -0.38354345 -0.90936875 -0.16106801 276.77746085 |
| 22027 | | -0.78111798 0.22638522 0.58189729 160.60505785 |
| 22028 | | -0.49269579 0.34899601 -0.79715284 242.75004629 |
| 22029 | | Axis -0.54793741 0.78020725 0.30173041 |
| 22030 | | Axis point 210.53234092 0.00000000 69.48347841 |
| 22031 | | Rotation angle (degrees) 167.72960946 |
| 22032 | | Shift along axis 46.89357823 |
| 22033 | | |
| 22034 | | |
| 22035 | | > fitmap #30.6 inMap #14 |
| 22036 | | |
| 22037 | | Fit molecule COPI_cyto_J4025_model F (#30.6) to map |
| 22038 | | W20_J4025_consensus_resampled.mrc (#14) using 2400 atoms |
| 22039 | | average map value = 0.3154, steps = 76 |
| 22040 | | shifted from previous position = 4.38 |
| 22041 | | rotated from previous position = 6.32 degrees |
| 22042 | | atoms outside contour = 716, contour level = 0.25978 |
| 22043 | | |
| 22044 | | Position of COPI_cyto_J4025_model F (#30.6) relative to |
| 22045 | | W20_J4025_consensus_resampled.mrc (#14) coordinates: |
| 22046 | | Matrix rotation and translation |
| 22047 | | -0.46916307 -0.86826583 -0.16124662 276.76831771 |
| 22048 | | -0.75844576 0.30262715 0.57721474 152.99334716 |
| 22049 | | -0.45237823 0.39310465 -0.80051401 236.07204224 |
| 22050 | | Axis -0.50923185 0.80524369 0.30375239 |
| 22051 | | Axis point 199.19186600 0.00000000 71.96275109 |
| 22052 | | Rotation angle (degrees) 169.58524914 |
| 22053 | | Shift along axis 53.96513192 |
| 22054 | | |
| 22055 | | |
| 22056 | | > fitmap #30.7 inMap #14 |
| 22057 | | |
| 22058 | | Fit molecule COPI_cyto_J4025_model G (#30.7) to map |
| 22059 | | W20_J4025_consensus_resampled.mrc (#14) using 2302 atoms |
| 22060 | | average map value = 0.2919, steps = 76 |
| 22061 | | shifted from previous position = 4.85 |
| 22062 | | rotated from previous position = 4.34 degrees |
| 22063 | | atoms outside contour = 830, contour level = 0.25978 |
| 22064 | | |
| 22065 | | Position of COPI_cyto_J4025_model G (#30.7) relative to |
| 22066 | | W20_J4025_consensus_resampled.mrc (#14) coordinates: |
| 22067 | | Matrix rotation and translation |
| 22068 | | -0.43043084 -0.88252203 -0.18943119 279.00638393 |
| 22069 | | -0.77202835 0.25122185 0.58383201 159.76771478 |
| 22070 | | -0.46765535 0.39754556 -0.78946565 235.51132473 |
| 22071 | | Axis -0.52833633 0.78908543 0.31337663 |
| 22072 | | Axis point 205.32244175 0.00000000 68.76349381 |
| 22073 | | Rotation angle (degrees) 169.84595276 |
| 22074 | | Shift along axis 52.46491169 |
| 22075 | | |
| 22076 | | |
| 22077 | | > fitmap #30.8 inMap #14 |
| 22078 | | |
| 22079 | | Fit molecule COPI_cyto_J4025_model H (#30.8) to map |
| 22080 | | W20_J4025_consensus_resampled.mrc (#14) using 420 atoms |
| 22081 | | average map value = 0.3536, steps = 80 |
| 22082 | | shifted from previous position = 3.69 |
| 22083 | | rotated from previous position = 5.02 degrees |
| 22084 | | atoms outside contour = 122, contour level = 0.25978 |
| 22085 | | |
| 22086 | | Position of COPI_cyto_J4025_model H (#30.8) relative to |
| 22087 | | W20_J4025_consensus_resampled.mrc (#14) coordinates: |
| 22088 | | Matrix rotation and translation |
| 22089 | | -0.35116806 -0.91592354 -0.19433237 278.04382885 |
| 22090 | | -0.84420748 0.21996160 0.48880532 174.65406664 |
| 22091 | | -0.40496263 0.33570965 -0.85047299 242.78401221 |
| 22092 | | Axis -0.56683990 0.77986296 0.26553022 |
| 22093 | | Axis point 212.55649174 0.00000000 78.50487362 |
| 22094 | | Rotation angle (degrees) 172.23888649 |
| 22095 | | Shift along axis 43.06639339 |
| 22096 | | |
| 22097 | | |
| 22098 | | > fitmap #30.9 inMap #14 |
| 22099 | | |
| 22100 | | Fit molecule COPI_cyto_J4025_model I (#30.9) to map |
| 22101 | | W20_J4025_consensus_resampled.mrc (#14) using 1573 atoms |
| 22102 | | average map value = 0.2801, steps = 68 |
| 22103 | | shifted from previous position = 1.75 |
| 22104 | | rotated from previous position = 3.58 degrees |
| 22105 | | atoms outside contour = 701, contour level = 0.25978 |
| 22106 | | |
| 22107 | | Position of COPI_cyto_J4025_model I (#30.9) relative to |
| 22108 | | W20_J4025_consensus_resampled.mrc (#14) coordinates: |
| 22109 | | Matrix rotation and translation |
| 22110 | | -0.38742239 -0.89858889 -0.20601436 277.54357463 |
| 22111 | | -0.78023576 0.20056569 0.59245724 165.02578227 |
| 22112 | | -0.49105608 0.39027097 -0.77881481 238.87427461 |
| 22113 | | Axis -0.54798153 0.77254303 0.32077019 |
| 22114 | | Axis point 210.67928153 0.00000000 66.74726373 |
| 22115 | | Rotation angle (degrees) 169.36902321 |
| 22116 | | Shift along axis 52.02451216 |
| 22117 | | |
| 22118 | | |
| 22119 | | > fitmap #30.10 inMap #14 |
| 22120 | | |
| 22121 | | Fit molecule COPI_cyto_J4025_model J (#30.10) to map |
| 22122 | | W20_J4025_consensus_resampled.mrc (#14) using 1112 atoms |
| 22123 | | average map value = 0.3297, steps = 72 |
| 22124 | | shifted from previous position = 0.309 |
| 22125 | | rotated from previous position = 4.19 degrees |
| 22126 | | atoms outside contour = 404, contour level = 0.25978 |
| 22127 | | |
| 22128 | | Position of COPI_cyto_J4025_model J (#30.10) relative to |
| 22129 | | W20_J4025_consensus_resampled.mrc (#14) coordinates: |
| 22130 | | Matrix rotation and translation |
| 22131 | | -0.35980949 -0.92767682 -0.09976404 268.82850280 |
| 22132 | | -0.80210592 0.25292807 0.54097459 164.87500239 |
| 22133 | | -0.47661645 0.27466912 -0.83510098 249.92805127 |
| 22134 | | Axis -0.55685591 0.78801420 0.26257402 |
| 22135 | | Axis point 210.98119167 0.00000000 74.83620925 |
| 22136 | | Rotation angle (degrees) 166.16567308 |
| 22137 | | Shift along axis 45.84971671 |
| 22138 | | |
| 22139 | | |
| 22140 | | > hide #!30 models |
| 22141 | | |
| 22142 | | > split #31 chains |
| 22143 | | |
| 22144 | | Split COPI_cyto_J4026_model (#31) into 13 models |
| 22145 | | Chain information for COPI_cyto_J4026_model A #31.1 |
| 22146 | | --- |
| 22147 | | Chain | Description |
| 22148 | | A | No description available |
| 22149 | | |
| 22150 | | Chain information for COPI_cyto_J4026_model B #31.2 |
| 22151 | | --- |
| 22152 | | Chain | Description |
| 22153 | | B | No description available |
| 22154 | | |
| 22155 | | Chain information for COPI_cyto_J4026_model C #31.3 |
| 22156 | | --- |
| 22157 | | Chain | Description |
| 22158 | | C | No description available |
| 22159 | | |
| 22160 | | Chain information for COPI_cyto_J4026_model D #31.4 |
| 22161 | | --- |
| 22162 | | Chain | Description |
| 22163 | | D | No description available |
| 22164 | | |
| 22165 | | Chain information for COPI_cyto_J4026_model E #31.5 |
| 22166 | | --- |
| 22167 | | Chain | Description |
| 22168 | | E | No description available |
| 22169 | | |
| 22170 | | Chain information for COPI_cyto_J4026_model F #31.6 |
| 22171 | | --- |
| 22172 | | Chain | Description |
| 22173 | | F | No description available |
| 22174 | | |
| 22175 | | Chain information for COPI_cyto_J4026_model G #31.7 |
| 22176 | | --- |
| 22177 | | Chain | Description |
| 22178 | | G | No description available |
| 22179 | | |
| 22180 | | Chain information for COPI_cyto_J4026_model H #31.8 |
| 22181 | | --- |
| 22182 | | Chain | Description |
| 22183 | | H | No description available |
| 22184 | | |
| 22185 | | Chain information for COPI_cyto_J4026_model I #31.9 |
| 22186 | | --- |
| 22187 | | Chain | Description |
| 22188 | | I | No description available |
| 22189 | | |
| 22190 | | Chain information for COPI_cyto_J4026_model J #31.10 |
| 22191 | | --- |
| 22192 | | Chain | Description |
| 22193 | | J | No description available |
| 22194 | | |
| 22195 | | Chain information for COPI_cyto_J4026_model K #31.11 |
| 22196 | | --- |
| 22197 | | Chain | Description |
| 22198 | | K | No description available |
| 22199 | | |
| 22200 | | Chain information for COPI_cyto_J4026_model L #31.12 |
| 22201 | | --- |
| 22202 | | Chain | Description |
| 22203 | | L | No description available |
| 22204 | | |
| 22205 | | Chain information for COPI_cyto_J4026_model M #31.13 |
| 22206 | | --- |
| 22207 | | Chain | Description |
| 22208 | | M | No description available |
| 22209 | | |
| 22210 | | |
| 22211 | | > show #31.1 models |
| 22212 | | |
| 22213 | | > show #31.2 models |
| 22214 | | |
| 22215 | | > show #31.3 models |
| 22216 | | |
| 22217 | | > show #!31.4 models |
| 22218 | | |
| 22219 | | > show #31.5 models |
| 22220 | | |
| 22221 | | > show #31.6 models |
| 22222 | | |
| 22223 | | > show #31.7 models |
| 22224 | | |
| 22225 | | > show #31.8 models |
| 22226 | | |
| 22227 | | > show #31.9 models |
| 22228 | | |
| 22229 | | > show #31.10 models |
| 22230 | | |
| 22231 | | > show #31.11 models |
| 22232 | | |
| 22233 | | > show #31.12 models |
| 22234 | | |
| 22235 | | > show #31.13 models |
| 22236 | | |
| 22237 | | > hide #!14 models |
| 22238 | | |
| 22239 | | > color #16 darkgrey models |
| 22240 | | |
| 22241 | | > color #16 #a9a9a9bf models |
| 22242 | | |
| 22243 | | > show #!16 models |
| 22244 | | |
| 22245 | | > fitmap #31.1 inMap #16 |
| 22246 | | |
| 22247 | | Fit molecule COPI_cyto_J4026_model A (#31.1) to map |
| 22248 | | W20_J4026_consensus_resampled.mrc (#16) using 2590 atoms |
| 22249 | | average map value = 0.3888, steps = 44 |
| 22250 | | shifted from previous position = 1.29 |
| 22251 | | rotated from previous position = 3.47 degrees |
| 22252 | | atoms outside contour = 601, contour level = 0.25963 |
| 22253 | | |
| 22254 | | Position of COPI_cyto_J4026_model A (#31.1) relative to |
| 22255 | | W20_J4026_consensus_resampled.mrc (#16) coordinates: |
| 22256 | | Matrix rotation and translation |
| 22257 | | -0.42179573 -0.88868235 -0.17981118 281.30883739 |
| 22258 | | -0.78142467 0.25572018 0.56919478 159.93267883 |
| 22259 | | -0.45985201 0.38059282 -0.80229998 237.51658930 |
| 22260 | | Axis -0.53238793 0.79050266 0.30276829 |
| 22261 | | Axis point 207.09091155 0.00000000 70.78659039 |
| 22262 | | Rotation angle (degrees) 169.79746249 |
| 22263 | | Shift along axis 48.57427120 |
| 22264 | | |
| 22265 | | |
| 22266 | | > fitmap #31.2 inMap #16 |
| 22267 | | |
| 22268 | | Fit molecule COPI_cyto_J4026_model B (#31.2) to map |
| 22269 | | W20_J4026_consensus_resampled.mrc (#16) using 2426 atoms |
| 22270 | | average map value = 0.3729, steps = 96 |
| 22271 | | shifted from previous position = 1.87 |
| 22272 | | rotated from previous position = 3.62 degrees |
| 22273 | | atoms outside contour = 599, contour level = 0.25963 |
| 22274 | | |
| 22275 | | Position of COPI_cyto_J4026_model B (#31.2) relative to |
| 22276 | | W20_J4026_consensus_resampled.mrc (#16) coordinates: |
| 22277 | | Matrix rotation and translation |
| 22278 | | -0.41831806 -0.88734205 -0.19399514 282.19270881 |
| 22279 | | -0.78598077 0.24658443 0.56694830 161.68081011 |
| 22280 | | -0.45524088 0.38964115 -0.80058449 236.37167847 |
| 22281 | | Axis -0.53469530 0.78782425 0.30566958 |
| 22282 | | Axis point 207.69640372 0.00000000 70.48893010 |
| 22283 | | Rotation angle (degrees) 170.45617020 |
| 22284 | | Shift along axis 48.74057955 |
| 22285 | | |
| 22286 | | |
| 22287 | | > fitmap #31.3 inMap #16 |
| 22288 | | |
| 22289 | | Fit molecule COPI_cyto_J4026_model C (#31.3) to map |
| 22290 | | W20_J4026_consensus_resampled.mrc (#16) using 1612 atoms |
| 22291 | | average map value = 0.3193, steps = 132 |
| 22292 | | shifted from previous position = 1.47 |
| 22293 | | rotated from previous position = 4.65 degrees |
| 22294 | | atoms outside contour = 561, contour level = 0.25963 |
| 22295 | | |
| 22296 | | Position of COPI_cyto_J4026_model C (#31.3) relative to |
| 22297 | | W20_J4026_consensus_resampled.mrc (#16) coordinates: |
| 22298 | | Matrix rotation and translation |
| 22299 | | -0.38059936 -0.91824851 -0.10937920 274.00313195 |
| 22300 | | -0.75305941 0.23911656 0.61296396 156.01911017 |
| 22301 | | -0.53669887 0.31566272 -0.78250328 246.73459431 |
| 22302 | | Axis -0.54436027 0.78242476 0.30246220 |
| 22303 | | Axis point 211.94174234 0.00000000 66.53857749 |
| 22304 | | Rotation angle (degrees) 164.15273201 |
| 22305 | | Shift along axis 47.54468445 |
| 22306 | | |
| 22307 | | |
| 22308 | | > fitmap #31.4 inMap #16 |
| 22309 | | |
| 22310 | | Fit molecule COPI_cyto_J4026_model D (#31.4) to map |
| 22311 | | W20_J4026_consensus_resampled.mrc (#16) using 4745 atoms |
| 22312 | | average map value = 0.2725, steps = 48 |
| 22313 | | shifted from previous position = 0.247 |
| 22314 | | rotated from previous position = 2.27 degrees |
| 22315 | | atoms outside contour = 1838, contour level = 0.25963 |
| 22316 | | |
| 22317 | | Position of COPI_cyto_J4026_model D (#31.4) relative to |
| 22318 | | W20_J4026_consensus_resampled.mrc (#16) coordinates: |
| 22319 | | Matrix rotation and translation |
| 22320 | | -0.36032627 -0.92283158 -0.13618688 272.90164046 |
| 22321 | | -0.80657650 0.23487967 0.54246282 165.85713835 |
| 22322 | | -0.46861429 0.30530874 -0.82896757 246.32646884 |
| 22323 | | Axis -0.55856608 0.78296216 0.27381417 |
| 22324 | | Axis point 211.65908561 0.00000000 73.73169378 |
| 22325 | | Rotation angle (degrees) 167.74351385 |
| 22326 | | Shift along axis 44.87394129 |
| 22327 | | |
| 22328 | | |
| 22329 | | > fitmap #31.5 inMap #16 |
| 22330 | | |
| 22331 | | Fit molecule COPI_cyto_J4026_model E (#31.5) to map |
| 22332 | | W20_J4026_consensus_resampled.mrc (#16) using 1876 atoms |
| 22333 | | average map value = 0.3337, steps = 68 |
| 22334 | | shifted from previous position = 0.775 |
| 22335 | | rotated from previous position = 3.95 degrees |
| 22336 | | atoms outside contour = 584, contour level = 0.25963 |
| 22337 | | |
| 22338 | | Position of COPI_cyto_J4026_model E (#31.5) relative to |
| 22339 | | W20_J4026_consensus_resampled.mrc (#16) coordinates: |
| 22340 | | Matrix rotation and translation |
| 22341 | | -0.41217203 -0.89272092 -0.18210871 281.24771125 |
| 22342 | | -0.76396069 0.22971791 0.60298736 156.90992899 |
| 22343 | | -0.49646580 0.38765842 -0.77668699 239.04221312 |
| 22344 | | Axis -0.53537564 0.78159084 0.32013853 |
| 22345 | | Axis point 208.88799304 0.00000000 67.41763702 |
| 22346 | | Rotation angle (degrees) 168.39866163 |
| 22347 | | Shift along axis 48.59281177 |
| 22348 | | |
| 22349 | | |
| 22350 | | > fitmap #31.10 inMap #16 |
| 22351 | | |
| 22352 | | Fit molecule COPI_cyto_J4026_model J (#31.10) to map |
| 22353 | | W20_J4026_consensus_resampled.mrc (#16) using 1112 atoms |
| 22354 | | average map value = 0.275, steps = 132 |
| 22355 | | shifted from previous position = 1.13 |
| 22356 | | rotated from previous position = 6.46 degrees |
| 22357 | | atoms outside contour = 480, contour level = 0.25963 |
| 22358 | | |
| 22359 | | Position of COPI_cyto_J4026_model J (#31.10) relative to |
| 22360 | | W20_J4026_consensus_resampled.mrc (#16) coordinates: |
| 22361 | | Matrix rotation and translation |
| 22362 | | -0.39165506 -0.91840010 -0.05610338 267.15064982 |
| 22363 | | -0.77630145 0.29709402 0.55595973 158.01112550 |
| 22364 | | -0.49392549 0.26129757 -0.82931369 252.31232484 |
| 22365 | | Axis -0.53914103 0.80107967 0.25999678 |
| 22366 | | Axis point 207.59429240 0.00000000 75.03244785 |
| 22367 | | Rotation angle (degrees) 164.14105481 |
| 22368 | | Shift along axis 48.14801546 |
| 22369 | | |
| 22370 | | |
| 22371 | | > fitmap #31.10 inMap #16 |
| 22372 | | |
| 22373 | | Fit molecule COPI_cyto_J4026_model J (#31.10) to map |
| 22374 | | W20_J4026_consensus_resampled.mrc (#16) using 1112 atoms |
| 22375 | | average map value = 0.275, steps = 48 |
| 22376 | | shifted from previous position = 0.0181 |
| 22377 | | rotated from previous position = 0.0479 degrees |
| 22378 | | atoms outside contour = 481, contour level = 0.25963 |
| 22379 | | |
| 22380 | | Position of COPI_cyto_J4026_model J (#31.10) relative to |
| 22381 | | W20_J4026_consensus_resampled.mrc (#16) coordinates: |
| 22382 | | Matrix rotation and translation |
| 22383 | | -0.39111474 -0.91864714 -0.05582756 267.08423778 |
| 22384 | | -0.77617700 0.29664639 0.55637235 158.00648729 |
| 22385 | | -0.49454882 0.26093749 -0.82905555 252.36987731 |
| 22386 | | Axis -0.53933760 0.80091724 0.26008948 |
| 22387 | | Axis point 207.64765628 0.00000000 74.98798493 |
| 22388 | | Rotation angle (degrees) 164.10431634 |
| 22389 | | Shift along axis 48.14029770 |
| 22390 | | |
| 22391 | | |
| 22392 | | > fitmap #31.12 inMap #16 |
| 22393 | | |
| 22394 | | Fit molecule COPI_cyto_J4026_model L (#31.12) to map |
| 22395 | | W20_J4026_consensus_resampled.mrc (#16) using 1420 atoms |
| 22396 | | average map value = 0.246, steps = 148 |
| 22397 | | shifted from previous position = 18 |
| 22398 | | rotated from previous position = 21.2 degrees |
| 22399 | | atoms outside contour = 781, contour level = 0.25963 |
| 22400 | | |
| 22401 | | Position of COPI_cyto_J4026_model L (#31.12) relative to |
| 22402 | | W20_J4026_consensus_resampled.mrc (#16) coordinates: |
| 22403 | | Matrix rotation and translation |
| 22404 | | -0.20471014 -0.86077408 -0.46600619 296.00976398 |
| 22405 | | -0.82456258 -0.10488916 0.55596297 187.90289621 |
| 22406 | | -0.52743751 0.49806252 -0.68829022 207.63206185 |
| 22407 | | Axis -0.63033876 0.66877783 0.39421981 |
| 22408 | | Axis point 239.98122179 0.00000000 43.12371251 |
| 22409 | | Rotation angle (degrees) 177.36759071 |
| 22410 | | Shift along axis 20.93153367 |
| 22411 | | |
| 22412 | | |
| 22413 | | > fitmap #31.13 inMap #16 |
| 22414 | | |
| 22415 | | Fit molecule COPI_cyto_J4026_model M (#31.13) to map |
| 22416 | | W20_J4026_consensus_resampled.mrc (#16) using 4552 atoms |
| 22417 | | average map value = 0.2784, steps = 240 |
| 22418 | | shifted from previous position = 13.3 |
| 22419 | | rotated from previous position = 28.4 degrees |
| 22420 | | atoms outside contour = 2174, contour level = 0.25963 |
| 22421 | | |
| 22422 | | Position of COPI_cyto_J4026_model M (#31.13) relative to |
| 22423 | | W20_J4026_consensus_resampled.mrc (#16) coordinates: |
| 22424 | | Matrix rotation and translation |
| 22425 | | -0.19059508 -0.97763486 0.08890226 232.38163718 |
| 22426 | | -0.95481798 0.20565637 0.21454160 224.73228555 |
| 22427 | | -0.22802666 -0.04399490 -0.97266043 250.13777446 |
| 22428 | | Axis -0.63112960 0.77367498 0.05569971 |
| 22429 | | Axis point 224.43338633 0.00000000 99.69760848 |
| 22430 | | Rotation angle (degrees) 168.18101146 |
| 22431 | | Shift along axis 41.13941809 |
| 22432 | | |
| 22433 | | |
| 22434 | | > hide #!31 models |
| 22435 | | |
| 22436 | | > show #!32 models |
| 22437 | | |
| 22438 | | > split #32 chains |
| 22439 | | |
| 22440 | | Split COPI_cyto_J4027_model (#32) into 13 models |
| 22441 | | Chain information for COPI_cyto_J4027_model A #32.1 |
| 22442 | | --- |
| 22443 | | Chain | Description |
| 22444 | | A | No description available |
| 22445 | | |
| 22446 | | Chain information for COPI_cyto_J4027_model B #32.2 |
| 22447 | | --- |
| 22448 | | Chain | Description |
| 22449 | | B | No description available |
| 22450 | | |
| 22451 | | Chain information for COPI_cyto_J4027_model C #32.3 |
| 22452 | | --- |
| 22453 | | Chain | Description |
| 22454 | | C | No description available |
| 22455 | | |
| 22456 | | Chain information for COPI_cyto_J4027_model D #32.4 |
| 22457 | | --- |
| 22458 | | Chain | Description |
| 22459 | | D | No description available |
| 22460 | | |
| 22461 | | Chain information for COPI_cyto_J4027_model E #32.5 |
| 22462 | | --- |
| 22463 | | Chain | Description |
| 22464 | | E | No description available |
| 22465 | | |
| 22466 | | Chain information for COPI_cyto_J4027_model F #32.6 |
| 22467 | | --- |
| 22468 | | Chain | Description |
| 22469 | | F | No description available |
| 22470 | | |
| 22471 | | Chain information for COPI_cyto_J4027_model G #32.7 |
| 22472 | | --- |
| 22473 | | Chain | Description |
| 22474 | | G | No description available |
| 22475 | | |
| 22476 | | Chain information for COPI_cyto_J4027_model H #32.8 |
| 22477 | | --- |
| 22478 | | Chain | Description |
| 22479 | | H | No description available |
| 22480 | | |
| 22481 | | Chain information for COPI_cyto_J4027_model I #32.9 |
| 22482 | | --- |
| 22483 | | Chain | Description |
| 22484 | | I | No description available |
| 22485 | | |
| 22486 | | Chain information for COPI_cyto_J4027_model J #32.10 |
| 22487 | | --- |
| 22488 | | Chain | Description |
| 22489 | | J | No description available |
| 22490 | | |
| 22491 | | Chain information for COPI_cyto_J4027_model K #32.11 |
| 22492 | | --- |
| 22493 | | Chain | Description |
| 22494 | | K | No description available |
| 22495 | | |
| 22496 | | Chain information for COPI_cyto_J4027_model L #32.12 |
| 22497 | | --- |
| 22498 | | Chain | Description |
| 22499 | | L | No description available |
| 22500 | | |
| 22501 | | Chain information for COPI_cyto_J4027_model M #32.13 |
| 22502 | | --- |
| 22503 | | Chain | Description |
| 22504 | | M | No description available |
| 22505 | | |
| 22506 | | |
| 22507 | | > hide #!16 models |
| 22508 | | |
| 22509 | | > show #!18 models |
| 22510 | | |
| 22511 | | > color #18 darkgrey models |
| 22512 | | |
| 22513 | | > color #18 #a9a9a9be models |
| 22514 | | |
| 22515 | | > color #18 #a9a9a9bf models |
| 22516 | | |
| 22517 | | > fitmap #32.1 inMap #18 |
| 22518 | | |
| 22519 | | Fit molecule COPI_cyto_J4027_model A (#32.1) to map |
| 22520 | | W20_J4027_consensus_resampled.mrc (#18) using 2590 atoms |
| 22521 | | average map value = 0.3669, steps = 68 |
| 22522 | | shifted from previous position = 0.35 |
| 22523 | | rotated from previous position = 0.41 degrees |
| 22524 | | atoms outside contour = 652, contour level = 0.25259 |
| 22525 | | |
| 22526 | | Position of COPI_cyto_J4027_model A (#32.1) relative to |
| 22527 | | W20_J4027_consensus_resampled.mrc (#18) coordinates: |
| 22528 | | Matrix rotation and translation |
| 22529 | | -0.36484420 -0.91715830 -0.16034147 275.00307794 |
| 22530 | | -0.79726014 0.21879492 0.56258783 164.15850506 |
| 22531 | | -0.48090019 0.33309077 -0.81103980 243.29869461 |
| 22532 | | Axis -0.55693597 0.77792142 0.29096492 |
| 22533 | | Axis point 211.66585714 0.00000000 70.85307433 |
| 22534 | | Rotation angle (degrees) 168.10988229 |
| 22535 | | Shift along axis 45.33469685 |
| 22536 | | |
| 22537 | | |
| 22538 | | > fitmap #32.2 inMap #18 |
| 22539 | | |
| 22540 | | Fit molecule COPI_cyto_J4027_model B (#32.2) to map |
| 22541 | | W20_J4027_consensus_resampled.mrc (#18) using 2426 atoms |
| 22542 | | average map value = 0.354, steps = 64 |
| 22543 | | shifted from previous position = 0.279 |
| 22544 | | rotated from previous position = 0.915 degrees |
| 22545 | | atoms outside contour = 634, contour level = 0.25259 |
| 22546 | | |
| 22547 | | Position of COPI_cyto_J4027_model B (#32.2) relative to |
| 22548 | | W20_J4027_consensus_resampled.mrc (#18) coordinates: |
| 22549 | | Matrix rotation and translation |
| 22550 | | -0.37575375 -0.90933745 -0.17864639 277.57875572 |
| 22551 | | -0.79848110 0.21984249 0.56044378 165.00984098 |
| 22552 | | -0.47035845 0.35323462 -0.80869540 240.85491140 |
| 22553 | | Axis -0.55315660 0.77874186 0.29593731 |
| 22554 | | Axis point 211.17574622 0.00000000 70.68321054 |
| 22555 | | Rotation angle (degrees) 169.20491693 |
| 22556 | | Shift along axis 46.23350389 |
| 22557 | | |
| 22558 | | |
| 22559 | | > fitmap #32.3 inMap #18 |
| 22560 | | |
| 22561 | | Fit molecule COPI_cyto_J4027_model C (#32.3) to map |
| 22562 | | W20_J4027_consensus_resampled.mrc (#18) using 1612 atoms |
| 22563 | | average map value = 0.307, steps = 124 |
| 22564 | | shifted from previous position = 0.916 |
| 22565 | | rotated from previous position = 4.06 degrees |
| 22566 | | atoms outside contour = 549, contour level = 0.25259 |
| 22567 | | |
| 22568 | | Position of COPI_cyto_J4027_model C (#32.3) relative to |
| 22569 | | W20_J4027_consensus_resampled.mrc (#18) coordinates: |
| 22570 | | Matrix rotation and translation |
| 22571 | | -0.38280043 -0.91660120 -0.11535204 274.19904393 |
| 22572 | | -0.75859054 0.24060781 0.60551491 157.21505378 |
| 22573 | | -0.52726108 0.31929633 -0.78742976 246.39824143 |
| 22574 | | Axis -0.54425835 0.78326479 0.30046485 |
| 22575 | | Axis point 211.53956724 0.00000000 67.39140608 |
| 22576 | | Rotation angle (degrees) 164.75519865 |
| 22577 | | Shift along axis 47.93990763 |
| 22578 | | |
| 22579 | | |
| 22580 | | > fitmap #32.4 inMap #18 |
| 22581 | | |
| 22582 | | Fit molecule COPI_cyto_J4027_model D (#32.4) to map |
| 22583 | | W20_J4027_consensus_resampled.mrc (#18) using 4745 atoms |
| 22584 | | average map value = 0.2737, steps = 64 |
| 22585 | | shifted from previous position = 0.424 |
| 22586 | | rotated from previous position = 0.652 degrees |
| 22587 | | atoms outside contour = 1745, contour level = 0.25259 |
| 22588 | | |
| 22589 | | Position of COPI_cyto_J4027_model D (#32.4) relative to |
| 22590 | | W20_J4027_consensus_resampled.mrc (#18) coordinates: |
| 22591 | | Matrix rotation and translation |
| 22592 | | -0.37438641 -0.91150980 -0.17024900 276.33175026 |
| 22593 | | -0.80075946 0.22523142 0.55502712 164.71539509 |
| 22594 | | -0.46756723 0.34412311 -0.81422367 240.98992687 |
| 22595 | | Axis -0.55358575 0.78040778 0.29070004 |
| 22596 | | Axis point 210.61143312 0.00000000 71.24312694 |
| 22597 | | Rotation angle (degrees) 169.01867576 |
| 22598 | | Shift along axis 45.62763928 |
| 22599 | | |
| 22600 | | |
| 22601 | | > fitmap #32.5 inMap #18 |
| 22602 | | |
| 22603 | | Fit molecule COPI_cyto_J4027_model E (#32.5) to map |
| 22604 | | W20_J4027_consensus_resampled.mrc (#18) using 1876 atoms |
| 22605 | | average map value = 0.3307, steps = 48 |
| 22606 | | shifted from previous position = 0.367 |
| 22607 | | rotated from previous position = 3.19 degrees |
| 22608 | | atoms outside contour = 589, contour level = 0.25259 |
| 22609 | | |
| 22610 | | Position of COPI_cyto_J4027_model E (#32.5) relative to |
| 22611 | | W20_J4027_consensus_resampled.mrc (#18) coordinates: |
| 22612 | | Matrix rotation and translation |
| 22613 | | -0.38890629 -0.90726627 -0.16006193 277.40112056 |
| 22614 | | -0.76465276 0.22097260 0.60537367 157.20851597 |
| 22615 | | -0.51386581 0.35782543 -0.77968129 242.84222727 |
| 22616 | | Axis -0.54436333 0.77802149 0.31360984 |
| 22617 | | Axis point 210.57407631 0.00000000 67.28775591 |
| 22618 | | Rotation angle (degrees) 166.85747662 |
| 22619 | | Shift along axis 47.46231881 |
| 22620 | | |
| 22621 | | |
| 22622 | | > fitmap #32.6 inMap #18 |
| 22623 | | |
| 22624 | | Fit molecule COPI_cyto_J4027_model F (#32.6) to map |
| 22625 | | W20_J4027_consensus_resampled.mrc (#18) using 2400 atoms |
| 22626 | | average map value = 0.1739, steps = 100 |
| 22627 | | shifted from previous position = 2.11 |
| 22628 | | rotated from previous position = 17.6 degrees |
| 22629 | | atoms outside contour = 2400, contour level = 0.25259 |
| 22630 | | |
| 22631 | | Position of COPI_cyto_J4027_model F (#32.6) relative to |
| 22632 | | W20_J4027_consensus_resampled.mrc (#18) coordinates: |
| 22633 | | Matrix rotation and translation |
| 22634 | | -0.22469468 -0.97442669 0.00222317 252.72099618 |
| 22635 | | -0.68062183 0.15857762 0.71526714 144.63194145 |
| 22636 | | -0.69732793 0.15920358 -0.69884762 265.52538785 |
| 22637 | | Axis -0.59112239 0.74365656 0.31232874 |
| 22638 | | Axis point 226.29273798 0.00000000 55.85483264 |
| 22639 | | Rotation angle (degrees) 151.94327126 |
| 22640 | | Shift along axis 41.09866330 |
| 22641 | | |
| 22642 | | |
| 22643 | | > ui tool show Matchmaker |
| 22644 | | |
| 22645 | | > matchmaker #32.6 to #33 |
| 22646 | | |
| 22647 | | Parameters |
| 22648 | | --- |
| 22649 | | Chain pairing | bb |
| 22650 | | Alignment algorithm | Needleman-Wunsch |
| 22651 | | Similarity matrix | BLOSUM-62 |
| 22652 | | SS fraction | 0.3 |
| 22653 | | Gap open (HH/SS/other) | 18/18/6 |
| 22654 | | Gap extend | 1 |
| 22655 | | SS matrix | | | H | S | O |
| 22656 | | ---|---|---|--- |
| 22657 | | H | 6 | -9 | -6 |
| 22658 | | S | | 6 | -6 |
| 22659 | | O | | | 4 |
| 22660 | | Iteration cutoff | 2 |
| 22661 | | |
| 22662 | | Matchmaker COPI_cyto_J4028_model, chain F (#33) with COPI_cyto_J4027_model F, |
| 22663 | | chain F (#32.6), sequence alignment score = 1592 |
| 22664 | | RMSD between 299 pruned atom pairs is 0.000 angstroms; (across all 299 pairs: |
| 22665 | | 0.000) |
| 22666 | | |
| 22667 | | |
| 22668 | | > hide #!32 models |
| 22669 | | |
| 22670 | | > show #!32 models |
| 22671 | | |
| 22672 | | > hide #!32 models |
| 22673 | | |
| 22674 | | > hide #!18 models |
| 22675 | | |
| 22676 | | > show #!20 models |
| 22677 | | |
| 22678 | | > color #20 darkgrey models |
| 22679 | | |
| 22680 | | > color #20 #a9a9a9c2 models |
| 22681 | | |
| 22682 | | > show #!33 models |
| 22683 | | |
| 22684 | | > split #33 chains |
| 22685 | | |
| 22686 | | Split COPI_cyto_J4028_model (#33) into 13 models |
| 22687 | | Chain information for COPI_cyto_J4028_model A #33.1 |
| 22688 | | --- |
| 22689 | | Chain | Description |
| 22690 | | A | No description available |
| 22691 | | |
| 22692 | | Chain information for COPI_cyto_J4028_model B #33.2 |
| 22693 | | --- |
| 22694 | | Chain | Description |
| 22695 | | B | No description available |
| 22696 | | |
| 22697 | | Chain information for COPI_cyto_J4028_model C #33.3 |
| 22698 | | --- |
| 22699 | | Chain | Description |
| 22700 | | C | No description available |
| 22701 | | |
| 22702 | | Chain information for COPI_cyto_J4028_model D #33.4 |
| 22703 | | --- |
| 22704 | | Chain | Description |
| 22705 | | D | No description available |
| 22706 | | |
| 22707 | | Chain information for COPI_cyto_J4028_model E #33.5 |
| 22708 | | --- |
| 22709 | | Chain | Description |
| 22710 | | E | No description available |
| 22711 | | |
| 22712 | | Chain information for COPI_cyto_J4028_model F #33.6 |
| 22713 | | --- |
| 22714 | | Chain | Description |
| 22715 | | F | No description available |
| 22716 | | |
| 22717 | | Chain information for COPI_cyto_J4028_model G #33.7 |
| 22718 | | --- |
| 22719 | | Chain | Description |
| 22720 | | G | No description available |
| 22721 | | |
| 22722 | | Chain information for COPI_cyto_J4028_model H #33.8 |
| 22723 | | --- |
| 22724 | | Chain | Description |
| 22725 | | H | No description available |
| 22726 | | |
| 22727 | | Chain information for COPI_cyto_J4028_model I #33.9 |
| 22728 | | --- |
| 22729 | | Chain | Description |
| 22730 | | I | No description available |
| 22731 | | |
| 22732 | | Chain information for COPI_cyto_J4028_model J #33.10 |
| 22733 | | --- |
| 22734 | | Chain | Description |
| 22735 | | J | No description available |
| 22736 | | |
| 22737 | | Chain information for COPI_cyto_J4028_model K #33.11 |
| 22738 | | --- |
| 22739 | | Chain | Description |
| 22740 | | K | No description available |
| 22741 | | |
| 22742 | | Chain information for COPI_cyto_J4028_model L #33.12 |
| 22743 | | --- |
| 22744 | | Chain | Description |
| 22745 | | L | No description available |
| 22746 | | |
| 22747 | | Chain information for COPI_cyto_J4028_model M #33.13 |
| 22748 | | --- |
| 22749 | | Chain | Description |
| 22750 | | M | No description available |
| 22751 | | |
| 22752 | | |
| 22753 | | > fitmap #33.1 inMap #20 |
| 22754 | | |
| 22755 | | Fit molecule COPI_cyto_J4028_model A (#33.1) to map |
| 22756 | | W20_J4028_consensus_resampled.mrc (#20) using 2590 atoms |
| 22757 | | average map value = 0.3839, steps = 84 |
| 22758 | | shifted from previous position = 1.3 |
| 22759 | | rotated from previous position = 3.69 degrees |
| 22760 | | atoms outside contour = 606, contour level = 0.25474 |
| 22761 | | |
| 22762 | | Position of COPI_cyto_J4028_model A (#33.1) relative to |
| 22763 | | W20_J4028_consensus_resampled.mrc (#20) coordinates: |
| 22764 | | Matrix rotation and translation |
| 22765 | | -0.42505705 -0.88684737 -0.18118571 281.77112295 |
| 22766 | | -0.78040543 0.25764379 0.56972543 159.62921913 |
| 22767 | | -0.45857812 0.38356412 -0.80161381 237.17247148 |
| 22768 | | Axis -0.53095306 0.79115444 0.30358443 |
| 22769 | | Axis point 206.86596376 0.00000000 70.78540234 |
| 22770 | | Rotation angle (degrees) 169.90338089 |
| 22771 | | Shift along axis 48.68599577 |
| 22772 | | |
| 22773 | | |
| 22774 | | > fitmap #33.2 inMap #20 |
| 22775 | | |
| 22776 | | Fit molecule COPI_cyto_J4028_model B (#33.2) to map |
| 22777 | | W20_J4028_consensus_resampled.mrc (#20) using 2426 atoms |
| 22778 | | average map value = 0.3703, steps = 76 |
| 22779 | | shifted from previous position = 2 |
| 22780 | | rotated from previous position = 3.77 degrees |
| 22781 | | atoms outside contour = 594, contour level = 0.25474 |
| 22782 | | |
| 22783 | | Position of COPI_cyto_J4028_model B (#33.2) relative to |
| 22784 | | W20_J4028_consensus_resampled.mrc (#20) coordinates: |
| 22785 | | Matrix rotation and translation |
| 22786 | | -0.42163847 -0.88604192 -0.19274527 282.36522569 |
| 22787 | | -0.78360399 0.24908325 0.56914176 161.05650807 |
| 22788 | | -0.45627383 0.39100801 -0.79932906 236.28096107 |
| 22789 | | Axis -0.53305638 0.78859614 0.30654042 |
| 22790 | | Axis point 207.42054850 0.00000000 70.38397992 |
| 22791 | | Rotation angle (degrees) 170.38149718 |
| 22792 | | Shift along axis 48.92162012 |
| 22793 | | |
| 22794 | | |
| 22795 | | > fitmap #33.3 inMap #20 |
| 22796 | | |
| 22797 | | Fit molecule COPI_cyto_J4028_model C (#33.3) to map |
| 22798 | | W20_J4028_consensus_resampled.mrc (#20) using 1612 atoms |
| 22799 | | average map value = 0.2936, steps = 120 |
| 22800 | | shifted from previous position = 1.59 |
| 22801 | | rotated from previous position = 5.86 degrees |
| 22802 | | atoms outside contour = 556, contour level = 0.25474 |
| 22803 | | |
| 22804 | | Position of COPI_cyto_J4028_model C (#33.3) relative to |
| 22805 | | W20_J4028_consensus_resampled.mrc (#20) coordinates: |
| 22806 | | Matrix rotation and translation |
| 22807 | | -0.38874794 -0.91700982 -0.08926390 273.70817090 |
| 22808 | | -0.74150605 0.25389180 0.62105374 153.76404568 |
| 22809 | | -0.54684900 0.30762308 -0.77866824 247.74574422 |
| 22810 | | Axis -0.53877892 0.78657655 0.30168628 |
| 22811 | | Axis point 211.55093832 0.00000000 66.06733162 |
| 22812 | | Rotation angle (degrees) 163.08987268 |
| 22813 | | Shift along axis 48.22049311 |
| 22814 | | |
| 22815 | | |
| 22816 | | > fitmap #33.4 inMap #20 |
| 22817 | | |
| 22818 | | Fit molecule COPI_cyto_J4028_model D (#33.4) to map |
| 22819 | | W20_J4028_consensus_resampled.mrc (#20) using 4745 atoms |
| 22820 | | average map value = 0.2743, steps = 52 |
| 22821 | | shifted from previous position = 0.325 |
| 22822 | | rotated from previous position = 2.63 degrees |
| 22823 | | atoms outside contour = 1777, contour level = 0.25474 |
| 22824 | | |
| 22825 | | Position of COPI_cyto_J4028_model D (#33.4) relative to |
| 22826 | | W20_J4028_consensus_resampled.mrc (#20) coordinates: |
| 22827 | | Matrix rotation and translation |
| 22828 | | -0.35425466 -0.92574203 -0.13230776 272.21729531 |
| 22829 | | -0.80879167 0.23228423 0.54027779 166.54524500 |
| 22830 | | -0.46942484 0.29840534 -0.83102021 247.09025195 |
| 22831 | | Axis -0.56108841 0.78203404 0.27129791 |
| 22832 | | Axis point 212.23977312 0.00000000 73.93457988 |
| 22833 | | Rotation angle (degrees) 167.55287093 |
| 22834 | | Shift along axis 44.54114989 |
| 22835 | | |
| 22836 | | |
| 22837 | | > fitmap #33.5 inMap #20 |
| 22838 | | |
| 22839 | | Fit molecule COPI_cyto_J4028_model E (#33.5) to map |
| 22840 | | W20_J4028_consensus_resampled.mrc (#20) using 1876 atoms |
| 22841 | | average map value = 0.3337, steps = 68 |
| 22842 | | shifted from previous position = 0.86 |
| 22843 | | rotated from previous position = 3.26 degrees |
| 22844 | | atoms outside contour = 607, contour level = 0.25474 |
| 22845 | | |
| 22846 | | Position of COPI_cyto_J4028_model E (#33.5) relative to |
| 22847 | | W20_J4028_consensus_resampled.mrc (#20) coordinates: |
| 22848 | | Matrix rotation and translation |
| 22849 | | -0.40812036 -0.89487089 -0.18067614 280.82990976 |
| 22850 | | -0.77162408 0.23236180 0.59211848 158.56716868 |
| 22851 | | -0.48788736 0.38106967 -0.78533550 239.27651010 |
| 22852 | | Axis -0.53761002 0.78256694 0.31394976 |
| 22853 | | Axis point 209.07381918 0.00000000 68.40687366 |
| 22854 | | Rotation angle (degrees) 168.68024136 |
| 22855 | | Shift along axis 48.23325430 |
| 22856 | | |
| 22857 | | |
| 22858 | | > fitmap #33.10 inMap #20 |
| 22859 | | |
| 22860 | | Fit molecule COPI_cyto_J4028_model J (#33.10) to map |
| 22861 | | W20_J4028_consensus_resampled.mrc (#20) using 1112 atoms |
| 22862 | | average map value = 0.2746, steps = 88 |
| 22863 | | shifted from previous position = 1.18 |
| 22864 | | rotated from previous position = 6.07 degrees |
| 22865 | | atoms outside contour = 487, contour level = 0.25474 |
| 22866 | | |
| 22867 | | Position of COPI_cyto_J4028_model J (#33.10) relative to |
| 22868 | | W20_J4028_consensus_resampled.mrc (#20) coordinates: |
| 22869 | | Matrix rotation and translation |
| 22870 | | -0.37976892 -0.92311743 -0.06024771 266.93454127 |
| 22871 | | -0.77672422 0.28281318 0.56277545 158.41114514 |
| 22872 | | -0.50246898 0.26052048 -0.82441132 252.69246283 |
| 22873 | | Axis -0.54431763 0.79637675 0.26363308 |
| 22874 | | Axis point 208.95699781 0.00000000 74.09614523 |
| 22875 | | Rotation angle (degrees) 163.88025316 |
| 22876 | | Shift along axis 47.47586703 |
| 22877 | | |
| 22878 | | |
| 22879 | | > fitmap #33.12 inMap #20 |
| 22880 | | |
| 22881 | | Fit molecule COPI_cyto_J4028_model L (#33.12) to map |
| 22882 | | W20_J4028_consensus_resampled.mrc (#20) using 1420 atoms |
| 22883 | | average map value = 0.2305, steps = 152 |
| 22884 | | shifted from previous position = 15.6 |
| 22885 | | rotated from previous position = 25.9 degrees |
| 22886 | | atoms outside contour = 812, contour level = 0.25474 |
| 22887 | | |
| 22888 | | Position of COPI_cyto_J4028_model L (#33.12) relative to |
| 22889 | | W20_J4028_consensus_resampled.mrc (#20) coordinates: |
| 22890 | | Matrix rotation and translation |
| 22891 | | -0.06107414 -0.89318761 -0.44551751 275.05032468 |
| 22892 | | -0.79982244 -0.22322988 0.55718265 197.31395144 |
| 22893 | | -0.59712146 0.39036435 -0.70075791 230.69793847 |
| 22894 | | Axis -0.68372085 0.62136335 0.38266615 |
| 22895 | | Axis point 0.00000000 233.57747065 184.11991319 |
| 22896 | | Rotation angle (degrees) 172.99286255 |
| 22897 | | Shift along axis 22.82630694 |
| 22898 | | |
| 22899 | | |
| 22900 | | > select add #33.12 |
| 22901 | | |
| 22902 | | 1420 atoms, 1441 bonds, 177 residues, 1 model selected |
| 22903 | | |
| 22904 | | > view matrix models |
| 22905 | | > #33.12,-0.71047,-0.45523,-0.53665,342.29,-0.62968,0.75173,0.19595,130.11,0.31422,0.47714,-0.82074,104.42 |
| 22906 | | |
| 22907 | | > view matrix models |
| 22908 | | > #33.12,-0.87976,-0.194,-0.43403,328.35,-0.31813,0.91866,0.23421,62.94,0.35329,0.34413,-0.86992,118.06 |
| 22909 | | |
| 22910 | | > fitmap #33.12 inMap #20 |
| 22911 | | |
| 22912 | | Fit molecule COPI_cyto_J4028_model L (#33.12) to map |
| 22913 | | W20_J4028_consensus_resampled.mrc (#20) using 1420 atoms |
| 22914 | | average map value = 0.219, steps = 148 |
| 22915 | | shifted from previous position = 4.64 |
| 22916 | | rotated from previous position = 34.8 degrees |
| 22917 | | atoms outside contour = 813, contour level = 0.25474 |
| 22918 | | |
| 22919 | | Position of COPI_cyto_J4028_model L (#33.12) relative to |
| 22920 | | W20_J4028_consensus_resampled.mrc (#20) coordinates: |
| 22921 | | Matrix rotation and translation |
| 22922 | | -0.65509662 -0.64625356 -0.39141380 328.46645180 |
| 22923 | | -0.75535360 0.57185725 0.32003163 147.76949721 |
| 22924 | | 0.01701124 0.50530746 -0.86277170 152.27605950 |
| 22925 | | Axis 0.40140909 -0.88487271 -0.23637052 |
| 22926 | | Axis point 188.23063104 0.00000000 79.03730027 |
| 22927 | | Rotation angle (degrees) 166.65689588 |
| 22928 | | Shift along axis -34.90134836 |
| 22929 | | |
| 22930 | | |
| 22931 | | > ui mousemode right "rotate selected models" |
| 22932 | | |
| 22933 | | > view matrix models |
| 22934 | | > #33.12,-0.03029,-0.7598,0.64945,102.21,-0.99925,0.0072683,-0.038101,287,0.024229,-0.65012,-0.75945,246.16 |
| 22935 | | |
| 22936 | | > fitmap #33.12 inMap #20 |
| 22937 | | |
| 22938 | | Fit molecule COPI_cyto_J4028_model L (#33.12) to map |
| 22939 | | W20_J4028_consensus_resampled.mrc (#20) using 1420 atoms |
| 22940 | | average map value = 0.2032, steps = 160 |
| 22941 | | shifted from previous position = 13.1 |
| 22942 | | rotated from previous position = 20.8 degrees |
| 22943 | | atoms outside contour = 909, contour level = 0.25474 |
| 22944 | | |
| 22945 | | Position of COPI_cyto_J4028_model L (#33.12) relative to |
| 22946 | | W20_J4028_consensus_resampled.mrc (#20) coordinates: |
| 22947 | | Matrix rotation and translation |
| 22948 | | 0.16998318 -0.52866209 0.83163822 33.05834902 |
| 22949 | | -0.98535039 -0.07936651 0.15094897 271.66299549 |
| 22950 | | -0.01379677 -0.84511382 -0.53440836 228.58308709 |
| 22951 | | Axis -0.71969674 0.61086190 -0.32997627 |
| 22952 | | Axis point 0.00000000 219.83009454 42.24482979 |
| 22953 | | Rotation angle (degrees) 136.21122723 |
| 22954 | | Shift along axis 66.72959442 |
| 22955 | | |
| 22956 | | |
| 22957 | | > ui mousemode right "translate selected models" |
| 22958 | | |
| 22959 | | > view matrix models |
| 22960 | | > #33.12,0.16998,-0.52866,0.83164,40.813,-0.98535,-0.079367,0.15095,266.5,-0.013797,-0.84511,-0.53441,239.35 |
| 22961 | | |
| 22962 | | > ui mousemode right "rotate selected models" |
| 22963 | | |
| 22964 | | > view matrix models |
| 22965 | | > #33.12,-0.43029,-0.87383,0.22644,246.31,-0.87355,0.46631,0.13952,199.93,-0.22751,-0.13778,-0.96398,263.72 |
| 22966 | | |
| 22967 | | > ui mousemode right "translate selected models" |
| 22968 | | |
| 22969 | | > view matrix models |
| 22970 | | > #33.12,-0.43029,-0.87383,0.22644,240.63,-0.87355,0.46631,0.13952,199.48,-0.22751,-0.13778,-0.96398,257.34 |
| 22971 | | |
| 22972 | | > fitmap #33.12 inMap #20 |
| 22973 | | |
| 22974 | | Fit molecule COPI_cyto_J4028_model L (#33.12) to map |
| 22975 | | W20_J4028_consensus_resampled.mrc (#20) using 1420 atoms |
| 22976 | | average map value = 0.259, steps = 136 |
| 22977 | | shifted from previous position = 2.22 |
| 22978 | | rotated from previous position = 19 degrees |
| 22979 | | atoms outside contour = 643, contour level = 0.25474 |
| 22980 | | |
| 22981 | | Position of COPI_cyto_J4028_model L (#33.12) relative to |
| 22982 | | W20_J4028_consensus_resampled.mrc (#20) coordinates: |
| 22983 | | Matrix rotation and translation |
| 22984 | | -0.11376158 -0.96773135 0.22484296 202.04835591 |
| 22985 | | -0.96542922 0.16110229 0.20492070 231.97393999 |
| 22986 | | -0.23453090 -0.19375786 -0.95260336 262.84035133 |
| 22987 | | Axis -0.65544634 0.75523227 0.00378481 |
| 22988 | | Axis point 228.77213631 0.00000000 107.04713970 |
| 22989 | | Rotation angle (degrees) 162.29431769 |
| 22990 | | Shift along axis 43.75715061 |
| 22991 | | |
| 22992 | | |
| 22993 | | > select subtract #33.12 |
| 22994 | | |
| 22995 | | Nothing selected |
| 22996 | | |
| 22997 | | > fitmap #33.13 inMap #20 |
| 22998 | | |
| 22999 | | Fit molecule COPI_cyto_J4028_model M (#33.13) to map |
| 23000 | | W20_J4028_consensus_resampled.mrc (#20) using 4552 atoms |
| 23001 | | average map value = 0.2597, steps = 164 |
| 23002 | | shifted from previous position = 11.9 |
| 23003 | | rotated from previous position = 21.2 degrees |
| 23004 | | atoms outside contour = 2429, contour level = 0.25474 |
| 23005 | | |
| 23006 | | Position of COPI_cyto_J4028_model M (#33.13) relative to |
| 23007 | | W20_J4028_consensus_resampled.mrc (#20) coordinates: |
| 23008 | | Matrix rotation and translation |
| 23009 | | -0.41064746 -0.90764367 0.08690026 262.06407158 |
| 23010 | | -0.84733447 0.41507760 0.33126258 174.12202496 |
| 23011 | | -0.33673873 0.06239856 -0.93952832 256.95292530 |
| 23012 | | Axis -0.53201816 0.83828111 0.11933760 |
| 23013 | | Axis point 205.62822196 0.00000000 94.49206914 |
| 23014 | | Rotation angle (degrees) 165.36365713 |
| 23015 | | Shift along axis 37.20450278 |
| 23016 | | |
| 23017 | | |
| 23018 | | > show #!32 models |
| 23019 | | |
| 23020 | | > hide #!33 models |
| 23021 | | |
| 23022 | | > hide #!32 models |
| 23023 | | |
| 23024 | | > show #!33 models |
| 23025 | | |
| 23026 | | > hide #!33 models |
| 23027 | | |
| 23028 | | > split #34 chains |
| 23029 | | |
| 23030 | | Split COPI_cyto_J3988_model (#34) into 13 models |
| 23031 | | Chain information for COPI_cyto_J3988_model A #34.1 |
| 23032 | | --- |
| 23033 | | Chain | Description |
| 23034 | | A | No description available |
| 23035 | | |
| 23036 | | Chain information for COPI_cyto_J3988_model B #34.2 |
| 23037 | | --- |
| 23038 | | Chain | Description |
| 23039 | | B | No description available |
| 23040 | | |
| 23041 | | Chain information for COPI_cyto_J3988_model C #34.3 |
| 23042 | | --- |
| 23043 | | Chain | Description |
| 23044 | | C | No description available |
| 23045 | | |
| 23046 | | Chain information for COPI_cyto_J3988_model D #34.4 |
| 23047 | | --- |
| 23048 | | Chain | Description |
| 23049 | | D | No description available |
| 23050 | | |
| 23051 | | Chain information for COPI_cyto_J3988_model E #34.5 |
| 23052 | | --- |
| 23053 | | Chain | Description |
| 23054 | | E | No description available |
| 23055 | | |
| 23056 | | Chain information for COPI_cyto_J3988_model F #34.6 |
| 23057 | | --- |
| 23058 | | Chain | Description |
| 23059 | | F | No description available |
| 23060 | | |
| 23061 | | Chain information for COPI_cyto_J3988_model G #34.7 |
| 23062 | | --- |
| 23063 | | Chain | Description |
| 23064 | | G | No description available |
| 23065 | | |
| 23066 | | Chain information for COPI_cyto_J3988_model H #34.8 |
| 23067 | | --- |
| 23068 | | Chain | Description |
| 23069 | | H | No description available |
| 23070 | | |
| 23071 | | Chain information for COPI_cyto_J3988_model I #34.9 |
| 23072 | | --- |
| 23073 | | Chain | Description |
| 23074 | | I | No description available |
| 23075 | | |
| 23076 | | Chain information for COPI_cyto_J3988_model J #34.10 |
| 23077 | | --- |
| 23078 | | Chain | Description |
| 23079 | | J | No description available |
| 23080 | | |
| 23081 | | Chain information for COPI_cyto_J3988_model K #34.11 |
| 23082 | | --- |
| 23083 | | Chain | Description |
| 23084 | | K | No description available |
| 23085 | | |
| 23086 | | Chain information for COPI_cyto_J3988_model L #34.12 |
| 23087 | | --- |
| 23088 | | Chain | Description |
| 23089 | | L | No description available |
| 23090 | | |
| 23091 | | Chain information for COPI_cyto_J3988_model M #34.13 |
| 23092 | | --- |
| 23093 | | Chain | Description |
| 23094 | | M | No description available |
| 23095 | | |
| 23096 | | |
| 23097 | | > show #34.1 models |
| 23098 | | |
| 23099 | | > show #34.2 models |
| 23100 | | |
| 23101 | | > show #34.3 models |
| 23102 | | |
| 23103 | | > show #!34.4 models |
| 23104 | | |
| 23105 | | > show #34.5 models |
| 23106 | | |
| 23107 | | > show #34.6 models |
| 23108 | | |
| 23109 | | > show #34.7 models |
| 23110 | | |
| 23111 | | > show #34.8 models |
| 23112 | | |
| 23113 | | > show #34.9 models |
| 23114 | | |
| 23115 | | > show #34.10 models |
| 23116 | | |
| 23117 | | > show #34.11 models |
| 23118 | | |
| 23119 | | > show #34.12 models |
| 23120 | | |
| 23121 | | > show #34.13 models |
| 23122 | | |
| 23123 | | > hide #!20 models |
| 23124 | | |
| 23125 | | > show #!22 models |
| 23126 | | |
| 23127 | | > color #22 darkgrey models |
| 23128 | | |
| 23129 | | > color #22 #a9a9a9bf models |
| 23130 | | |
| 23131 | | > fitmap #34.1 inMap #22 |
| 23132 | | |
| 23133 | | Fit molecule COPI_cyto_J3988_model A (#34.1) to map |
| 23134 | | W20_J3988_consensus_resampled.mrc (#22) using 2590 atoms |
| 23135 | | average map value = 0.3688, steps = 88 |
| 23136 | | shifted from previous position = 1.25 |
| 23137 | | rotated from previous position = 3.5 degrees |
| 23138 | | atoms outside contour = 749, contour level = 0.28583 |
| 23139 | | |
| 23140 | | Position of COPI_cyto_J3988_model A (#34.1) relative to |
| 23141 | | W20_J3988_consensus_resampled.mrc (#22) coordinates: |
| 23142 | | Matrix rotation and translation |
| 23143 | | -0.41785370 -0.88839960 -0.19011695 282.28257009 |
| 23144 | | -0.78880679 0.25093956 0.56108217 162.05511406 |
| 23145 | | -0.45075731 0.38441580 -0.80563165 235.85823021 |
| 23146 | | Axis -0.53495212 0.78922836 0.30157060 |
| 23147 | | Axis point 207.72235198 0.00000000 70.74799729 |
| 23148 | | Rotation angle (degrees) 170.49558915 |
| 23149 | | Shift along axis 48.01874106 |
| 23150 | | |
| 23151 | | |
| 23152 | | > fitmap #34.2 inMap #22 |
| 23153 | | |
| 23154 | | Fit molecule COPI_cyto_J3988_model B (#34.2) to map |
| 23155 | | W20_J3988_consensus_resampled.mrc (#22) using 2426 atoms |
| 23156 | | average map value = 0.3348, steps = 104 |
| 23157 | | shifted from previous position = 1.68 |
| 23158 | | rotated from previous position = 3.88 degrees |
| 23159 | | atoms outside contour = 828, contour level = 0.28583 |
| 23160 | | |
| 23161 | | Position of COPI_cyto_J3988_model B (#34.2) relative to |
| 23162 | | W20_J3988_consensus_resampled.mrc (#22) coordinates: |
| 23163 | | Matrix rotation and translation |
| 23164 | | -0.42101840 -0.88538396 -0.19707555 283.10669504 |
| 23165 | | -0.78443773 0.24632045 0.56919566 160.85163308 |
| 23166 | | -0.45541296 0.39423534 -0.79823402 235.48770247 |
| 23167 | | Axis -0.53352603 0.78777711 0.30782658 |
| 23168 | | Axis point 207.58242660 0.00000000 70.13942680 |
| 23169 | | Rotation angle (degrees) 170.56282406 |
| 23170 | | Shift along axis 48.15981832 |
| 23171 | | |
| 23172 | | |
| 23173 | | > fitmap #34.3 inMap #22 |
| 23174 | | |
| 23175 | | Fit molecule COPI_cyto_J3988_model C (#34.3) to map |
| 23176 | | W20_J3988_consensus_resampled.mrc (#22) using 1612 atoms |
| 23177 | | average map value = 0.3235, steps = 80 |
| 23178 | | shifted from previous position = 1.01 |
| 23179 | | rotated from previous position = 1.39 degrees |
| 23180 | | atoms outside contour = 647, contour level = 0.28583 |
| 23181 | | |
| 23182 | | Position of COPI_cyto_J3988_model C (#34.3) relative to |
| 23183 | | W20_J3988_consensus_resampled.mrc (#22) coordinates: |
| 23184 | | Matrix rotation and translation |
| 23185 | | -0.38953007 -0.90460759 -0.17306487 278.08945587 |
| 23186 | | -0.79075902 0.23214543 0.56639976 162.53472037 |
| 23187 | | -0.47219330 0.35748234 -0.80575423 240.06559363 |
| 23188 | | Axis -0.54660128 0.78262495 0.29786781 |
| 23189 | | Axis point 209.73775516 0.00000000 70.39505904 |
| 23190 | | Rotation angle (degrees) 168.98267188 |
| 23191 | | Shift along axis 46.70748572 |
| 23192 | | |
| 23193 | | |
| 23194 | | > fitmap #34.4 inMap #22 |
| 23195 | | |
| 23196 | | Fit molecule COPI_cyto_J3988_model D (#34.4) to map |
| 23197 | | W20_J3988_consensus_resampled.mrc (#22) using 4745 atoms |
| 23198 | | average map value = 0.341, steps = 72 |
| 23199 | | shifted from previous position = 0.451 |
| 23200 | | rotated from previous position = 2.53 degrees |
| 23201 | | atoms outside contour = 1760, contour level = 0.28583 |
| 23202 | | |
| 23203 | | Position of COPI_cyto_J3988_model D (#34.4) relative to |
| 23204 | | W20_J3988_consensus_resampled.mrc (#22) coordinates: |
| 23205 | | Matrix rotation and translation |
| 23206 | | -0.35609850 -0.92473928 -0.13435451 272.54654270 |
| 23207 | | -0.80894027 0.23309315 0.53970662 166.76836324 |
| 23208 | | -0.46777080 0.30087349 -0.83106296 247.02887626 |
| 23209 | | Axis -0.56042334 0.78236325 0.27172306 |
| 23210 | | Axis point 212.17904341 0.00000000 74.04958983 |
| 23211 | | Rotation angle (degrees) 167.69692699 |
| 23212 | | Shift along axis 44.85543655 |
| 23213 | | |
| 23214 | | |
| 23215 | | > fitmap #34.5 inMap #22 |
| 23216 | | |
| 23217 | | Fit molecule COPI_cyto_J3988_model E (#34.5) to map |
| 23218 | | W20_J3988_consensus_resampled.mrc (#22) using 1876 atoms |
| 23219 | | average map value = 0.3399, steps = 68 |
| 23220 | | shifted from previous position = 0.902 |
| 23221 | | rotated from previous position = 1.88 degrees |
| 23222 | | atoms outside contour = 644, contour level = 0.28583 |
| 23223 | | |
| 23224 | | Position of COPI_cyto_J3988_model E (#34.5) relative to |
| 23225 | | W20_J3988_consensus_resampled.mrc (#22) coordinates: |
| 23226 | | Matrix rotation and translation |
| 23227 | | -0.38559316 -0.90595853 -0.17480580 278.32647192 |
| 23228 | | -0.80563868 0.23824013 0.54239099 166.10780051 |
| 23229 | | -0.44973798 0.34997256 -0.82173899 240.13441550 |
| 23230 | | Axis -0.54937100 0.78495483 0.28642174 |
| 23231 | | Axis point 210.04384962 0.00000000 72.68833745 |
| 23232 | | Rotation angle (degrees) 169.91400249 |
| 23233 | | Shift along axis 46.26234424 |
| 23234 | | |
| 23235 | | |
| 23236 | | > fitmap #34.10 inMap #22 |
| 23237 | | |
| 23238 | | Fit molecule COPI_cyto_J3988_model J (#34.10) to map |
| 23239 | | W20_J3988_consensus_resampled.mrc (#22) using 1112 atoms |
| 23240 | | average map value = 0.314, steps = 56 |
| 23241 | | shifted from previous position = 0.967 |
| 23242 | | rotated from previous position = 5.85 degrees |
| 23243 | | atoms outside contour = 470, contour level = 0.28583 |
| 23244 | | |
| 23245 | | Position of COPI_cyto_J3988_model J (#34.10) relative to |
| 23246 | | W20_J3988_consensus_resampled.mrc (#22) coordinates: |
| 23247 | | Matrix rotation and translation |
| 23248 | | -0.37577208 -0.92422463 -0.06785421 266.88773036 |
| 23249 | | -0.79027325 0.28134549 0.54434632 161.90561717 |
| 23250 | | -0.48400780 0.25817351 -0.83611178 252.25402649 |
| 23251 | | Axis -0.54768370 0.79644372 0.25635904 |
| 23252 | | Axis point 209.14918816 0.00000000 75.62770199 |
| 23253 | | Rotation angle (degrees) 164.85531677 |
| 23254 | | Shift along axis 47.44625196 |
| 23255 | | |
| 23256 | | |
| 23257 | | > fitmap #34.12 inMap #22 |
| 23258 | | |
| 23259 | | Fit molecule COPI_cyto_J3988_model L (#34.12) to map |
| 23260 | | W20_J3988_consensus_resampled.mrc (#22) using 1420 atoms |
| 23261 | | average map value = 0.2477, steps = 128 |
| 23262 | | shifted from previous position = 15.9 |
| 23263 | | rotated from previous position = 23.9 degrees |
| 23264 | | atoms outside contour = 825, contour level = 0.28583 |
| 23265 | | |
| 23266 | | Position of COPI_cyto_J3988_model L (#34.12) relative to |
| 23267 | | W20_J3988_consensus_resampled.mrc (#22) coordinates: |
| 23268 | | Matrix rotation and translation |
| 23269 | | -0.13744140 -0.87706877 -0.46028280 286.39333689 |
| 23270 | | -0.79755718 -0.17757218 0.57651599 190.01595705 |
| 23271 | | -0.58737759 0.44633901 -0.67510671 220.17263411 |
| 23272 | | Axis -0.65564558 0.64012190 0.40046576 |
| 23273 | | Axis point 0.00000000 242.83430967 190.87489306 |
| 23274 | | Rotation angle (degrees) 174.30264049 |
| 23275 | | Shift along axis 22.03245138 |
| 23276 | | |
| 23277 | | |
| 23278 | | > ui mousemode right "rotate selected models" |
| 23279 | | |
| 23280 | | > select add #34.12 |
| 23281 | | |
| 23282 | | 1420 atoms, 1441 bonds, 177 residues, 1 model selected |
| 23283 | | |
| 23284 | | > view matrix models |
| 23285 | | > #34.12,0.094052,-0.95592,0.27815,157.02,-0.86993,0.056954,0.48988,190.57,-0.48413,-0.28805,-0.82622,295.38 |
| 23286 | | |
| 23287 | | > fitmap #34.12 inMap #22 |
| 23288 | | |
| 23289 | | Fit molecule COPI_cyto_J3988_model L (#34.12) to map |
| 23290 | | W20_J3988_consensus_resampled.mrc (#22) using 1420 atoms |
| 23291 | | average map value = 0.265, steps = 180 |
| 23292 | | shifted from previous position = 7.48 |
| 23293 | | rotated from previous position = 16.7 degrees |
| 23294 | | atoms outside contour = 709, contour level = 0.28583 |
| 23295 | | |
| 23296 | | Position of COPI_cyto_J3988_model L (#34.12) relative to |
| 23297 | | W20_J3988_consensus_resampled.mrc (#22) coordinates: |
| 23298 | | Matrix rotation and translation |
| 23299 | | -0.10354097 -0.97915690 0.17473139 207.46054459 |
| 23300 | | -0.94372455 0.15219348 0.29363435 218.75648454 |
| 23301 | | -0.31410708 -0.13449511 -0.93981265 267.23328820 |
| 23302 | | Axis -0.65787226 0.75115891 0.05444605 |
| 23303 | | Axis point 229.01761440 0.00000000 99.48866389 |
| 23304 | | Rotation angle (degrees) 161.01082491 |
| 23305 | | Shift along axis 42.38814162 |
| 23306 | | |
| 23307 | | |
| 23308 | | > select subtract #34.12 |
| 23309 | | |
| 23310 | | Nothing selected |
| 23311 | | |
| 23312 | | > fitmap #34.13 inMap #22 |
| 23313 | | |
| 23314 | | Fit molecule COPI_cyto_J3988_model M (#34.13) to map |
| 23315 | | W20_J3988_consensus_resampled.mrc (#22) using 4552 atoms |
| 23316 | | average map value = 0.2757, steps = 176 |
| 23317 | | shifted from previous position = 11.7 |
| 23318 | | rotated from previous position = 23.1 degrees |
| 23319 | | atoms outside contour = 2512, contour level = 0.28583 |
| 23320 | | |
| 23321 | | Position of COPI_cyto_J3988_model M (#34.13) relative to |
| 23322 | | W20_J3988_consensus_resampled.mrc (#22) coordinates: |
| 23323 | | Matrix rotation and translation |
| 23324 | | -0.23360378 -0.97224105 0.01329047 246.50433978 |
| 23325 | | -0.93813795 0.22896058 0.25975806 213.83868979 |
| 23326 | | -0.25559044 0.04821217 -0.96558227 246.39586728 |
| 23327 | | Axis -0.61528240 0.78204162 0.09918904 |
| 23328 | | Axis point 220.79513963 0.00000000 94.62595818 |
| 23329 | | Rotation angle (degrees) 170.10113474 |
| 23330 | | Shift along axis 40.00074310 |
| 23331 | | |
| 23332 | | |
| 23333 | | > save "/Users/becca/Desktop/Postdoc/COPI notebooks and |
| 23334 | | > resources/Processing/W20_MM_cyto_COPI/20250707_W20_COPI_cyto_alignments.cxs" |
| 23335 | | |
| 23336 | | > open |
| 23337 | | > /Users/becca/Desktop/Postdoc/Phenix/W20_MM_cyto_COPI/ResolveCryoEM_29/denmod_map.ccp4 |
| 23338 | | |
| 23339 | | Opened denmod_map.ccp4 as #35, grid size 99,98,133, pixel 1.19, shown at level |
| 23340 | | 0.318, step 1, values float32 |
| 23341 | | |
| 23342 | | > open |
| 23343 | | > /Users/becca/Desktop/Postdoc/Phenix/W20_MM_cyto_COPI/ResolveCryoEM_30/denmod_map.ccp4 |
| 23344 | | |
| 23345 | | Opened denmod_map.ccp4 as #36, grid size 97,98,137, pixel 1.19, shown at level |
| 23346 | | 0.404, step 1, values float32 |
| 23347 | | |
| 23348 | | > hide #!34 models |
| 23349 | | |
| 23350 | | > hide #!22 models |
| 23351 | | |
| 23352 | | > hide #!36 models |
| 23353 | | |
| 23354 | | > volume #35 level 0.105 |
| 23355 | | |
| 23356 | | > show #!34 models |
| 23357 | | |
| 23358 | | > hide #!35 models |
| 23359 | | |
| 23360 | | > show #!36 models |
| 23361 | | |
| 23362 | | > volume #36 level 0.1681 |
| 23363 | | |
| 23364 | | > hide #!36 models |
| 23365 | | |
| 23366 | | > close #35-36 |
| 23367 | | |
| 23368 | | > open |
| 23369 | | > /Users/becca/Desktop/Postdoc/Phenix/W20_MM_cyto_COPI/ResolveCryoEM_29/denmod_map.ccp4 |
| 23370 | | |
| 23371 | | Opened denmod_map.ccp4 as #35, grid size 99,98,133, pixel 1.19, shown at level |
| 23372 | | 0.318, step 1, values float32 |
| 23373 | | |
| 23374 | | > close #35 |
| 23375 | | |
| 23376 | | > show #!47 models |
| 23377 | | |
| 23378 | | > hide #!47 models |
| 23379 | | |
| 23380 | | > close #56#47-55,57 |
| 23381 | | |
| 23382 | | > show #!94 models |
| 23383 | | |
| 23384 | | > hide #!94 models |
| 23385 | | |
| 23386 | | > close #71-72,76,78-80,83,86-88#58-70,73-75,77,81-82,84-85,89-95 |
| 23387 | | |
| 23388 | | > open "/Users/becca/Desktop/Postdoc/COPI notebooks and resources/Structure |
| 23389 | | > files/COPI_soluble_job155_dhelix.pdb" |
| 23390 | | |
| 23391 | | Chain information for COPI_soluble_job155_dhelix.pdb #35 |
| 23392 | | --- |
| 23393 | | Chain | Description |
| 23394 | | D | No description available |
| 23395 | | E | No description available |
| 23396 | | F | No description available |
| 23397 | | G | No description available |
| 23398 | | H | No description available |
| 23399 | | I | No description available |
| 23400 | | J | No description available |
| 23401 | | K | No description available |
| 23402 | | L | No description available |
| 23403 | | M | No description available |
| 23404 | | N | No description available |
| 23405 | | O | No description available |
| 23406 | | P | No description available |
| 23407 | | Q | No description available |
| 23408 | | |
| 23409 | | |
| 23410 | | > open "/Users/becca/Desktop/Postdoc/COPI notebooks and resources/Structure |
| 23411 | | > files/COPI_soluble_job160_dhelix.pdb" |
| 23412 | | |
| 23413 | | Chain information for COPI_soluble_job160_dhelix.pdb #36 |
| 23414 | | --- |
| 23415 | | Chain | Description |
| 23416 | | D | No description available |
| 23417 | | E | No description available |
| 23418 | | F | No description available |
| 23419 | | G | No description available |
| 23420 | | H | No description available |
| 23421 | | I | No description available |
| 23422 | | J | No description available |
| 23423 | | K | No description available |
| 23424 | | L | No description available |
| 23425 | | M | No description available |
| 23426 | | N | No description available |
| 23427 | | O | No description available |
| 23428 | | P | No description available |
| 23429 | | Q | No description available |
| 23430 | | |
| 23431 | | |
| 23432 | | > ui tool show Matchmaker |
| 23433 | | |
| 23434 | | > matchmaker #!35-36 to #34.4 |
| 23435 | | |
| 23436 | | Parameters |
| 23437 | | --- |
| 23438 | | Chain pairing | bb |
| 23439 | | Alignment algorithm | Needleman-Wunsch |
| 23440 | | Similarity matrix | BLOSUM-62 |
| 23441 | | SS fraction | 0.3 |
| 23442 | | Gap open (HH/SS/other) | 18/18/6 |
| 23443 | | Gap extend | 1 |
| 23444 | | SS matrix | | | H | S | O |
| 23445 | | ---|---|---|--- |
| 23446 | | H | 6 | -9 | -6 |
| 23447 | | S | | 6 | -6 |
| 23448 | | O | | | 4 |
| 23449 | | Iteration cutoff | 2 |
| 23450 | | |
| 23451 | | Matchmaker COPI_cyto_J3988_model D, chain D (#34.4) with |
| 23452 | | COPI_soluble_job155_dhelix.pdb, chain I (#35), sequence alignment score = 1535 |
| 23453 | | RMSD between 291 pruned atom pairs is 0.541 angstroms; (across all 305 pairs: |
| 23454 | | 0.988) |
| 23455 | | |
| 23456 | | Matchmaker COPI_cyto_J3988_model D, chain D (#34.4) with |
| 23457 | | COPI_soluble_job160_dhelix.pdb, chain I (#36), sequence alignment score = 1538 |
| 23458 | | RMSD between 291 pruned atom pairs is 0.541 angstroms; (across all 305 pairs: |
| 23459 | | 0.988) |
| 23460 | | |
| 23461 | | |
| 23462 | | > hide #34.1-3,5-13#!35-36#!34.4 atoms |
| 23463 | | |
| 23464 | | > show #34.1-3,5-13#!35-36#!34.4 cartoons |
| 23465 | | |
| 23466 | | > hide #!34 models |
| 23467 | | |
| 23468 | | > hide #!36 models |
| 23469 | | |
| 23470 | | > show #!16 models |
| 23471 | | |
| 23472 | | > show #!18 models |
| 23473 | | |
| 23474 | | > hide #!16 models |
| 23475 | | |
| 23476 | | > hide #!18 models |
| 23477 | | |
| 23478 | | > show #!20 models |
| 23479 | | |
| 23480 | | > hide #!20 models |
| 23481 | | |
| 23482 | | > show #!22 models |
| 23483 | | |
| 23484 | | > hide #!35 models |
| 23485 | | |
| 23486 | | > show #!36 models |
| 23487 | | |
| 23488 | | > show #!20 models |
| 23489 | | |
| 23490 | | > hide #!20 models |
| 23491 | | |
| 23492 | | > show #!18 models |
| 23493 | | |
| 23494 | | > hide #!22 models |
| 23495 | | |
| 23496 | | > hide #!18 models |
| 23497 | | |
| 23498 | | > hide #!36 models |
| 23499 | | |
| 23500 | | > split #35 chains |
| 23501 | | |
| 23502 | | Split COPI_soluble_job155_dhelix.pdb (#35) into 14 models |
| 23503 | | Chain information for COPI_soluble_job155_dhelix.pdb D #35.1 |
| 23504 | | --- |
| 23505 | | Chain | Description |
| 23506 | | D | No description available |
| 23507 | | |
| 23508 | | Chain information for COPI_soluble_job155_dhelix.pdb E #35.2 |
| 23509 | | --- |
| 23510 | | Chain | Description |
| 23511 | | E | No description available |
| 23512 | | |
| 23513 | | Chain information for COPI_soluble_job155_dhelix.pdb F #35.3 |
| 23514 | | --- |
| 23515 | | Chain | Description |
| 23516 | | F | No description available |
| 23517 | | |
| 23518 | | Chain information for COPI_soluble_job155_dhelix.pdb G #35.4 |
| 23519 | | --- |
| 23520 | | Chain | Description |
| 23521 | | G | No description available |
| 23522 | | |
| 23523 | | Chain information for COPI_soluble_job155_dhelix.pdb H #35.5 |
| 23524 | | --- |
| 23525 | | Chain | Description |
| 23526 | | H | No description available |
| 23527 | | |
| 23528 | | Chain information for COPI_soluble_job155_dhelix.pdb I #35.6 |
| 23529 | | --- |
| 23530 | | Chain | Description |
| 23531 | | I | No description available |
| 23532 | | |
| 23533 | | Chain information for COPI_soluble_job155_dhelix.pdb J #35.7 |
| 23534 | | --- |
| 23535 | | Chain | Description |
| 23536 | | J | No description available |
| 23537 | | |
| 23538 | | Chain information for COPI_soluble_job155_dhelix.pdb K #35.8 |
| 23539 | | --- |
| 23540 | | Chain | Description |
| 23541 | | K | No description available |
| 23542 | | |
| 23543 | | Chain information for COPI_soluble_job155_dhelix.pdb L #35.9 |
| 23544 | | --- |
| 23545 | | Chain | Description |
| 23546 | | L | No description available |
| 23547 | | |
| 23548 | | Chain information for COPI_soluble_job155_dhelix.pdb M #35.10 |
| 23549 | | --- |
| 23550 | | Chain | Description |
| 23551 | | M | No description available |
| 23552 | | |
| 23553 | | Chain information for COPI_soluble_job155_dhelix.pdb N #35.11 |
| 23554 | | --- |
| 23555 | | Chain | Description |
| 23556 | | N | No description available |
| 23557 | | |
| 23558 | | Chain information for COPI_soluble_job155_dhelix.pdb O #35.12 |
| 23559 | | --- |
| 23560 | | Chain | Description |
| 23561 | | O | No description available |
| 23562 | | |
| 23563 | | Chain information for COPI_soluble_job155_dhelix.pdb P #35.13 |
| 23564 | | --- |
| 23565 | | Chain | Description |
| 23566 | | P | No description available |
| 23567 | | |
| 23568 | | Chain information for COPI_soluble_job155_dhelix.pdb Q #35.14 |
| 23569 | | --- |
| 23570 | | Chain | Description |
| 23571 | | Q | No description available |
| 23572 | | |
| 23573 | | |
| 23574 | | > split #36 chains |
| 23575 | | |
| 23576 | | Split COPI_soluble_job160_dhelix.pdb (#36) into 14 models |
| 23577 | | Chain information for COPI_soluble_job160_dhelix.pdb D #36.1 |
| 23578 | | --- |
| 23579 | | Chain | Description |
| 23580 | | D | No description available |
| 23581 | | |
| 23582 | | Chain information for COPI_soluble_job160_dhelix.pdb E #36.2 |
| 23583 | | --- |
| 23584 | | Chain | Description |
| 23585 | | E | No description available |
| 23586 | | |
| 23587 | | Chain information for COPI_soluble_job160_dhelix.pdb F #36.3 |
| 23588 | | --- |
| 23589 | | Chain | Description |
| 23590 | | F | No description available |
| 23591 | | |
| 23592 | | Chain information for COPI_soluble_job160_dhelix.pdb G #36.4 |
| 23593 | | --- |
| 23594 | | Chain | Description |
| 23595 | | G | No description available |
| 23596 | | |
| 23597 | | Chain information for COPI_soluble_job160_dhelix.pdb H #36.5 |
| 23598 | | --- |
| 23599 | | Chain | Description |
| 23600 | | H | No description available |
| 23601 | | |
| 23602 | | Chain information for COPI_soluble_job160_dhelix.pdb I #36.6 |
| 23603 | | --- |
| 23604 | | Chain | Description |
| 23605 | | I | No description available |
| 23606 | | |
| 23607 | | Chain information for COPI_soluble_job160_dhelix.pdb J #36.7 |
| 23608 | | --- |
| 23609 | | Chain | Description |
| 23610 | | J | No description available |
| 23611 | | |
| 23612 | | Chain information for COPI_soluble_job160_dhelix.pdb K #36.8 |
| 23613 | | --- |
| 23614 | | Chain | Description |
| 23615 | | K | No description available |
| 23616 | | |
| 23617 | | Chain information for COPI_soluble_job160_dhelix.pdb L #36.9 |
| 23618 | | --- |
| 23619 | | Chain | Description |
| 23620 | | L | No description available |
| 23621 | | |
| 23622 | | Chain information for COPI_soluble_job160_dhelix.pdb M #36.10 |
| 23623 | | --- |
| 23624 | | Chain | Description |
| 23625 | | M | No description available |
| 23626 | | |
| 23627 | | Chain information for COPI_soluble_job160_dhelix.pdb N #36.11 |
| 23628 | | --- |
| 23629 | | Chain | Description |
| 23630 | | N | No description available |
| 23631 | | |
| 23632 | | Chain information for COPI_soluble_job160_dhelix.pdb O #36.12 |
| 23633 | | --- |
| 23634 | | Chain | Description |
| 23635 | | O | No description available |
| 23636 | | |
| 23637 | | Chain information for COPI_soluble_job160_dhelix.pdb P #36.13 |
| 23638 | | --- |
| 23639 | | Chain | Description |
| 23640 | | P | No description available |
| 23641 | | |
| 23642 | | Chain information for COPI_soluble_job160_dhelix.pdb Q #36.14 |
| 23643 | | --- |
| 23644 | | Chain | Description |
| 23645 | | Q | No description available |
| 23646 | | |
| 23647 | | |
| 23648 | | > open |
| 23649 | | > /Users/becca/Desktop/Postdoc/Phenix/W20_MM_cyto_COPI/ResolveCryoEM_32/J4028_denmod_map.ccp4 |
| 23650 | | |
| 23651 | | Opened J4028_denmod_map.ccp4 as #37, grid size 98,99,135, pixel 1.19, shown at |
| 23652 | | level 0.424, step 1, values float32 |
| 23653 | | |
| 23654 | | > volume #37 level 0.2276 |
| 23655 | | |
| 23656 | | > hide #!35 models |
| 23657 | | |
| 23658 | | > hide #!36 models |
| 23659 | | |
| 23660 | | > show #!35.14 models |
| 23661 | | |
| 23662 | | > show #!35.13 models |
| 23663 | | |
| 23664 | | > show #35.12 models |
| 23665 | | |
| 23666 | | > show #!35.11 models |
| 23667 | | |
| 23668 | | > show #35.10 models |
| 23669 | | |
| 23670 | | > show #35.9 models |
| 23671 | | |
| 23672 | | > show #35.8 models |
| 23673 | | |
| 23674 | | > show #!35.7 models |
| 23675 | | |
| 23676 | | > show #!35.6 models |
| 23677 | | |
| 23678 | | > show #35.5 models |
| 23679 | | |
| 23680 | | > show #35.4 models |
| 23681 | | |
| 23682 | | > show #35.3 models |
| 23683 | | |
| 23684 | | > show #35.2 models |
| 23685 | | |
| 23686 | | > show #35.1 models |
| 23687 | | |
| 23688 | | > hide #!35 models |
| 23689 | | |
| 23690 | | > volume #37 level 0.1206 |
| 23691 | | |
| 23692 | | > close #37 |
| 23693 | | |
| 23694 | | > show #!16 models |
| 23695 | | |
| 23696 | | > show #!31 models |
| 23697 | | |
| 23698 | | > show #!35 models |
| 23699 | | |
| 23700 | | > hide #35.1 models |
| 23701 | | |
| 23702 | | > hide #35.2 models |
| 23703 | | |
| 23704 | | > hide #35.3 models |
| 23705 | | |
| 23706 | | > hide #35.4 models |
| 23707 | | |
| 23708 | | > hide #35.5 models |
| 23709 | | |
| 23710 | | > hide #!35.6 models |
| 23711 | | |
| 23712 | | > hide #!35.7 models |
| 23713 | | |
| 23714 | | > hide #35.8 models |
| 23715 | | |
| 23716 | | > hide #35.9 models |
| 23717 | | |
| 23718 | | > show #35.9 models |
| 23719 | | |
| 23720 | | > hide #35.10 models |
| 23721 | | |
| 23722 | | > show #35.10 models |
| 23723 | | |
| 23724 | | > hide #35.10 models |
| 23725 | | |
| 23726 | | > show #35.10 models |
| 23727 | | |
| 23728 | | > hide #!35.11 models |
| 23729 | | |
| 23730 | | > show #!35.11 models |
| 23731 | | |
| 23732 | | > hide #35.12 models |
| 23733 | | |
| 23734 | | > hide #!35.13 models |
| 23735 | | |
| 23736 | | > hide #!35.14 models |
| 23737 | | |
| 23738 | | > fitmap #35.9 inMap #16 |
| 23739 | | |
| 23740 | | Fit molecule COPI_soluble_job155_dhelix.pdb L (#35.9) to map |
| 23741 | | W20_J4026_consensus_resampled.mrc (#16) using 1163 atoms |
| 23742 | | average map value = 0.3245, steps = 56 |
| 23743 | | shifted from previous position = 1.07 |
| 23744 | | rotated from previous position = 3.98 degrees |
| 23745 | | atoms outside contour = 440, contour level = 0.25963 |
| 23746 | | |
| 23747 | | Position of COPI_soluble_job155_dhelix.pdb L (#35.9) relative to |
| 23748 | | W20_J4026_consensus_resampled.mrc (#16) coordinates: |
| 23749 | | Matrix rotation and translation |
| 23750 | | -0.90987894 0.24236896 -0.33671591 435.50395252 |
| 23751 | | 0.06606031 -0.71661672 -0.69433170 604.82381864 |
| 23752 | | -0.40958070 -0.65400135 0.63602349 262.39041137 |
| 23753 | | Axis 0.20683441 0.37368744 -0.90419977 |
| 23754 | | Axis point 263.30917938 345.30538105 0.00000000 |
| 23755 | | Rotation angle (degrees) 174.40510072 |
| 23756 | | Shift along axis 78.83891898 |
| 23757 | | |
| 23758 | | |
| 23759 | | > fitmap #35.10 inMap #16 |
| 23760 | | |
| 23761 | | Fit molecule COPI_soluble_job155_dhelix.pdb M (#35.10) to map |
| 23762 | | W20_J4026_consensus_resampled.mrc (#16) using 2212 atoms |
| 23763 | | average map value = 0.3306, steps = 92 |
| 23764 | | shifted from previous position = 2.46 |
| 23765 | | rotated from previous position = 6.1 degrees |
| 23766 | | atoms outside contour = 710, contour level = 0.25963 |
| 23767 | | |
| 23768 | | Position of COPI_soluble_job155_dhelix.pdb M (#35.10) relative to |
| 23769 | | W20_J4026_consensus_resampled.mrc (#16) coordinates: |
| 23770 | | Matrix rotation and translation |
| 23771 | | -0.91287694 0.27628102 -0.30054033 410.29266882 |
| 23772 | | -0.00773154 -0.74776328 -0.66392040 629.67159952 |
| 23773 | | -0.40816163 -0.60375398 0.68475193 227.42100406 |
| 23774 | | Axis 0.19432248 0.34758987 -0.91728952 |
| 23775 | | Axis point 254.63604518 341.25031476 0.00000000 |
| 23776 | | Rotation angle (degrees) 171.09417945 |
| 23777 | | Shift along axis 89.98565405 |
| 23778 | | |
| 23779 | | |
| 23780 | | > fitmap #35.11 inMap #16 |
| 23781 | | |
| 23782 | | Fit molecule COPI_soluble_job155_dhelix.pdb N (#35.11) to map |
| 23783 | | W20_J4026_consensus_resampled.mrc (#16) using 1064 atoms |
| 23784 | | average map value = 0.3024, steps = 52 |
| 23785 | | shifted from previous position = 1.19 |
| 23786 | | rotated from previous position = 1.61 degrees |
| 23787 | | atoms outside contour = 298, contour level = 0.25963 |
| 23788 | | |
| 23789 | | Position of COPI_soluble_job155_dhelix.pdb N (#35.11) relative to |
| 23790 | | W20_J4026_consensus_resampled.mrc (#16) coordinates: |
| 23791 | | Matrix rotation and translation |
| 23792 | | -0.93792901 0.21017775 -0.27588855 435.11677592 |
| 23793 | | 0.06068204 -0.68374702 -0.72719166 606.90260953 |
| 23794 | | -0.34147748 -0.69879563 0.62855215 261.30949009 |
| 23795 | | Axis 0.17136788 0.39582419 -0.90219524 |
| 23796 | | Axis point 255.61353944 351.07885546 0.00000000 |
| 23797 | | Rotation angle (degrees) 175.24753470 |
| 23798 | | Shift along axis 79.03959464 |
| 23799 | | |
| 23800 | | |
| 23801 | | > hide #31.13 models |
| 23802 | | |
| 23803 | | > hide #31.12 models |
| 23804 | | |
| 23805 | | > show #31.12 models |
| 23806 | | |
| 23807 | | > select add #31.12 |
| 23808 | | |
| 23809 | | 1420 atoms, 1441 bonds, 177 residues, 1 model selected |
| 23810 | | |
| 23811 | | > view matrix models |
| 23812 | | > #31.12,-0.23372,-0.97024,-0.063385,254.71,-0.96642,0.23897,-0.094492,266.72,0.10683,0.039172,-0.99351,199.68 |
| 23813 | | |
| 23814 | | > fitmap #31.12 inMap #16 |
| 23815 | | |
| 23816 | | Fit molecule COPI_cyto_J4026_model L (#31.12) to map |
| 23817 | | W20_J4026_consensus_resampled.mrc (#16) using 1420 atoms |
| 23818 | | average map value = 0.2882, steps = 136 |
| 23819 | | shifted from previous position = 4.88 |
| 23820 | | rotated from previous position = 24.5 degrees |
| 23821 | | atoms outside contour = 593, contour level = 0.25963 |
| 23822 | | |
| 23823 | | Position of COPI_cyto_J4026_model L (#31.12) relative to |
| 23824 | | W20_J4026_consensus_resampled.mrc (#16) coordinates: |
| 23825 | | Matrix rotation and translation |
| 23826 | | -0.11780824 -0.96709435 0.22549890 202.70773070 |
| 23827 | | -0.96549277 0.16465999 0.20176923 232.00719445 |
| 23828 | | -0.23226052 -0.19394747 -0.95312090 262.37235731 |
| 23829 | | Axis -0.65397629 0.75651041 0.00264684 |
| 23830 | | Axis point 228.48470686 0.00000000 107.10492224 |
| 23831 | | Rotation angle (degrees) 162.38937332 |
| 23832 | | Shift along axis 43.64426578 |
| 23833 | | |
| 23834 | | |
| 23835 | | > select subtract #31.12 |
| 23836 | | |
| 23837 | | Nothing selected |
| 23838 | | |
| 23839 | | > show #31.13 models |
| 23840 | | |
| 23841 | | > hide #31.13 models |
| 23842 | | |
| 23843 | | > hide #31.12 models |
| 23844 | | |
| 23845 | | > hide #31.11 models |
| 23846 | | |
| 23847 | | > hide #31.10 models |
| 23848 | | |
| 23849 | | > hide #31.9 models |
| 23850 | | |
| 23851 | | > hide #31.8 models |
| 23852 | | |
| 23853 | | > hide #31.7 models |
| 23854 | | |
| 23855 | | > hide #31.6 models |
| 23856 | | |
| 23857 | | > hide #31.5 models |
| 23858 | | |
| 23859 | | > hide #!31.4 models |
| 23860 | | |
| 23861 | | > hide #31.3 models |
| 23862 | | |
| 23863 | | > hide #31.2 models |
| 23864 | | |
| 23865 | | > hide #31.1 models |
| 23866 | | |
| 23867 | | > hide #!16 models |
| 23868 | | |
| 23869 | | > show #31.13 models |
| 23870 | | |
| 23871 | | > hide #35.9 models |
| 23872 | | |
| 23873 | | > hide #!35.11 models |
| 23874 | | |
| 23875 | | > hide #35.10 models |
| 23876 | | |
| 23877 | | > ui mousemode right select |
| 23878 | | |
| 23879 | | Drag select of 228 residues |
| 23880 | | |
| 23881 | | > select up |
| 23882 | | |
| 23883 | | 2019 atoms, 2046 bonds, 257 residues, 1 model selected |
| 23884 | | |
| 23885 | | > delete sel |
| 23886 | | |
| 23887 | | > show #35.10 models |
| 23888 | | |
| 23889 | | Drag select of 6 residues, 1 pseudobonds |
| 23890 | | |
| 23891 | | > select up |
| 23892 | | |
| 23893 | | 61 atoms, 60 bonds, 1 pseudobond, 8 residues, 2 models selected |
| 23894 | | |
| 23895 | | > delete sel |
| 23896 | | |
| 23897 | | Drag select of 3 residues |
| 23898 | | |
| 23899 | | > select up |
| 23900 | | |
| 23901 | | 130 atoms, 130 bonds, 17 residues, 1 model selected |
| 23902 | | |
| 23903 | | > delete sel |
| 23904 | | |
| 23905 | | > fitmap #31.13 inMap #16 |
| 23906 | | |
| 23907 | | Fit molecule COPI_cyto_J4026_model M (#31.13) to map |
| 23908 | | W20_J4026_consensus_resampled.mrc (#16) using 2342 atoms |
| 23909 | | average map value = 0.3254, steps = 72 |
| 23910 | | shifted from previous position = 2.25 |
| 23911 | | rotated from previous position = 5.92 degrees |
| 23912 | | atoms outside contour = 789, contour level = 0.25963 |
| 23913 | | |
| 23914 | | Position of COPI_cyto_J4026_model M (#31.13) relative to |
| 23915 | | W20_J4026_consensus_resampled.mrc (#16) coordinates: |
| 23916 | | Matrix rotation and translation |
| 23917 | | -0.14741636 -0.97480443 0.16740596 215.74618933 |
| 23918 | | -0.97007287 0.17551559 0.16778831 236.68236999 |
| 23919 | | -0.19294315 -0.13766124 -0.97150519 252.55946622 |
| 23920 | | Axis -0.64657421 0.76278534 0.01001574 |
| 23921 | | Axis point 227.98326837 0.00000000 105.57175219 |
| 23922 | | Rotation angle (degrees) 166.33727095 |
| 23923 | | Shift along axis 43.57149027 |
| 23924 | | |
| 23925 | | |
| 23926 | | > hide #!31.13 models |
| 23927 | | |
| 23928 | | > show #!31.13 models |
| 23929 | | |
| 23930 | | > show #!16 models |
| 23931 | | |
| 23932 | | > open "/Users/becca/Desktop/Postdoc/COPI notebooks and resources/Alphafold |
| 23933 | | > predictions/fold_20250401_ct_alpha1_350_gamma1_350/fold_20250401_ct_alpha1_350_gamma1_350_model_0.cif" |
| 23934 | | |
| 23935 | | Chain information for fold_20250401_ct_alpha1_350_gamma1_350_model_0.cif #37 |
| 23936 | | --- |
| 23937 | | Chain | Description |
| 23938 | | A | . |
| 23939 | | B | . |
| 23940 | | |
| 23941 | | Computing secondary structure |
| 23942 | | |
| 23943 | | > ui tool show Matchmaker |
| 23944 | | |
| 23945 | | > matchmaker #37 to #31.1 |
| 23946 | | |
| 23947 | | Parameters |
| 23948 | | --- |
| 23949 | | Chain pairing | bb |
| 23950 | | Alignment algorithm | Needleman-Wunsch |
| 23951 | | Similarity matrix | BLOSUM-62 |
| 23952 | | SS fraction | 0.3 |
| 23953 | | Gap open (HH/SS/other) | 18/18/6 |
| 23954 | | Gap extend | 1 |
| 23955 | | SS matrix | | | H | S | O |
| 23956 | | ---|---|---|--- |
| 23957 | | H | 6 | -9 | -6 |
| 23958 | | S | | 6 | -6 |
| 23959 | | O | | | 4 |
| 23960 | | Iteration cutoff | 2 |
| 23961 | | |
| 23962 | | Matchmaker COPI_cyto_J4026_model A, chain A (#31.1) with |
| 23963 | | fold_20250401_ct_alpha1_350_gamma1_350_model_0.cif, chain A (#37), sequence |
| 23964 | | alignment score = 1357.6 |
| 23965 | | RMSD between 278 pruned atom pairs is 0.471 angstroms; (across all 313 pairs: |
| 23966 | | 6.653) |
| 23967 | | |
| 23968 | | |
| 23969 | | > open "/Users/becca/Desktop/Postdoc/COPI notebooks and resources/Alphafold |
| 23970 | | > predictions/fold_20250401_ct_alpha1_350_gamma1_50/fold_20250401_ct_alpha1_350_gamma1_50_model_0.cif" |
| 23971 | | |
| 23972 | | Chain information for fold_20250401_ct_alpha1_350_gamma1_50_model_0.cif #38 |
| 23973 | | --- |
| 23974 | | Chain | Description |
| 23975 | | A | . |
| 23976 | | B | . |
| 23977 | | |
| 23978 | | Computing secondary structure |
| 23979 | | |
| 23980 | | > ui tool show Matchmaker |
| 23981 | | |
| 23982 | | > matchmaker #38 to #24.1 |
| 23983 | | |
| 23984 | | Parameters |
| 23985 | | --- |
| 23986 | | Chain pairing | bb |
| 23987 | | Alignment algorithm | Needleman-Wunsch |
| 23988 | | Similarity matrix | BLOSUM-62 |
| 23989 | | SS fraction | 0.3 |
| 23990 | | Gap open (HH/SS/other) | 18/18/6 |
| 23991 | | Gap extend | 1 |
| 23992 | | SS matrix | | | H | S | O |
| 23993 | | ---|---|---|--- |
| 23994 | | H | 6 | -9 | -6 |
| 23995 | | S | | 6 | -6 |
| 23996 | | O | | | 4 |
| 23997 | | Iteration cutoff | 2 |
| 23998 | | |
| 23999 | | Matchmaker COPI_cyto_for_J4027_v1 A, chain A (#24.1) with |
| 24000 | | fold_20250401_ct_alpha1_350_gamma1_50_model_0.cif, chain A (#38), sequence |
| 24001 | | alignment score = 1354 |
| 24002 | | RMSD between 279 pruned atom pairs is 0.445 angstroms; (across all 313 pairs: |
| 24003 | | 6.676) |
| 24004 | | |
| 24005 | | |
| 24006 | | > hide #!16 models |
| 24007 | | |
| 24008 | | > open "/Users/becca/Desktop/Postdoc/COPI notebooks and resources/Alphafold |
| 24009 | | > predictions/fold_2025_01_17_alpha1_330gamma1_330zeta/fold_2025_01_17_alpha1_330gamma1_330zeta_model_0.cif" |
| 24010 | | |
| 24011 | | Chain information for fold_2025_01_17_alpha1_330gamma1_330zeta_model_0.cif #39 |
| 24012 | | --- |
| 24013 | | Chain | Description |
| 24014 | | A | . |
| 24015 | | B | . |
| 24016 | | C | . |
| 24017 | | |
| 24018 | | Computing secondary structure |
| 24019 | | |
| 24020 | | > ui tool show Matchmaker |
| 24021 | | |
| 24022 | | > matchmaker #39 to #24.1 |
| 24023 | | |
| 24024 | | Parameters |
| 24025 | | --- |
| 24026 | | Chain pairing | bb |
| 24027 | | Alignment algorithm | Needleman-Wunsch |
| 24028 | | Similarity matrix | BLOSUM-62 |
| 24029 | | SS fraction | 0.3 |
| 24030 | | Gap open (HH/SS/other) | 18/18/6 |
| 24031 | | Gap extend | 1 |
| 24032 | | SS matrix | | | H | S | O |
| 24033 | | ---|---|---|--- |
| 24034 | | H | 6 | -9 | -6 |
| 24035 | | S | | 6 | -6 |
| 24036 | | O | | | 4 |
| 24037 | | Iteration cutoff | 2 |
| 24038 | | |
| 24039 | | Matchmaker COPI_cyto_for_J4027_v1 A, chain A (#24.1) with |
| 24040 | | fold_2025_01_17_alpha1_330gamma1_330zeta_model_0.cif, chain B (#39), sequence |
| 24041 | | alignment score = 1688.7 |
| 24042 | | RMSD between 283 pruned atom pairs is 0.307 angstroms; (across all 318 pairs: |
| 24043 | | 4.917) |
| 24044 | | |
| 24045 | | |
| 24046 | | > hide #38 models |
| 24047 | | |
| 24048 | | > hide #37 models |
| 24049 | | |
| 24050 | | > fitmap #39 inMap #16 |
| 24051 | | |
| 24052 | | Fit molecule fold_2025_01_17_alpha1_330gamma1_330zeta_model_0.cif (#39) to map |
| 24053 | | W20_J4026_consensus_resampled.mrc (#16) using 6676 atoms |
| 24054 | | average map value = 0.2973, steps = 112 |
| 24055 | | shifted from previous position = 5.99 |
| 24056 | | rotated from previous position = 14.7 degrees |
| 24057 | | atoms outside contour = 2828, contour level = 0.25963 |
| 24058 | | |
| 24059 | | Position of fold_2025_01_17_alpha1_330gamma1_330zeta_model_0.cif (#39) |
| 24060 | | relative to W20_J4026_consensus_resampled.mrc (#16) coordinates: |
| 24061 | | Matrix rotation and translation |
| 24062 | | 0.75236864 0.61844958 -0.22685138 135.89900591 |
| 24063 | | 0.54481378 -0.77776674 -0.31345947 141.53555984 |
| 24064 | | -0.37029633 0.11224532 -0.92210716 89.13598609 |
| 24065 | | Axis 0.93516725 0.31511280 -0.16175949 |
| 24066 | | Axis point 0.00000000 40.80728347 61.99249889 |
| 24067 | | Rotation angle (degrees) 166.84366143 |
| 24068 | | Shift along axis 157.26937502 |
| 24069 | | |
| 24070 | | |
| 24071 | | > split #39 chains |
| 24072 | | |
| 24073 | | Split fold_2025_01_17_alpha1_330gamma1_330zeta_model_0.cif (#39) into 3 models |
| 24074 | | Chain information for fold_2025_01_17_alpha1_330gamma1_330zeta_model_0.cif A |
| 24075 | | #39.1 |
| 24076 | | --- |
| 24077 | | Chain | Description |
| 24078 | | A | No description available |
| 24079 | | |
| 24080 | | Chain information for fold_2025_01_17_alpha1_330gamma1_330zeta_model_0.cif B |
| 24081 | | #39.2 |
| 24082 | | --- |
| 24083 | | Chain | Description |
| 24084 | | B | No description available |
| 24085 | | |
| 24086 | | Chain information for fold_2025_01_17_alpha1_330gamma1_330zeta_model_0.cif C |
| 24087 | | #39.3 |
| 24088 | | --- |
| 24089 | | Chain | Description |
| 24090 | | C | No description available |
| 24091 | | |
| 24092 | | |
| 24093 | | > show #!16 models |
| 24094 | | |
| 24095 | | > hide #!31.13 models |
| 24096 | | |
| 24097 | | > hide #39.2 models |
| 24098 | | |
| 24099 | | > show #39.2 models |
| 24100 | | |
| 24101 | | > hide #39.2 models |
| 24102 | | |
| 24103 | | > hide #39.1 models |
| 24104 | | |
| 24105 | | > show #39.1 models |
| 24106 | | |
| 24107 | | > hide #39.3 models |
| 24108 | | |
| 24109 | | > show #31.12 models |
| 24110 | | |
| 24111 | | > show #31.1 models |
| 24112 | | |
| 24113 | | > fitmap #39.1 inMap #16 |
| 24114 | | |
| 24115 | | Fit molecule fold_2025_01_17_alpha1_330gamma1_330zeta_model_0.cif A (#39.1) to |
| 24116 | | map W20_J4026_consensus_resampled.mrc (#16) using 2567 atoms |
| 24117 | | average map value = 0.332, steps = 92 |
| 24118 | | shifted from previous position = 1.08 |
| 24119 | | rotated from previous position = 8.07 degrees |
| 24120 | | atoms outside contour = 837, contour level = 0.25963 |
| 24121 | | |
| 24122 | | Position of fold_2025_01_17_alpha1_330gamma1_330zeta_model_0.cif A (#39.1) |
| 24123 | | relative to W20_J4026_consensus_resampled.mrc (#16) coordinates: |
| 24124 | | Matrix rotation and translation |
| 24125 | | 0.83404122 0.50639742 -0.21894494 135.37114526 |
| 24126 | | 0.43420964 -0.84733835 -0.30574452 138.37406207 |
| 24127 | | -0.34034868 0.15993553 -0.92659775 89.77783066 |
| 24128 | | Axis 0.95695074 0.24947901 -0.14834252 |
| 24129 | | Axis point 0.00000000 44.30433155 61.88415197 |
| 24130 | | Rotation angle (degrees) 165.91774988 |
| 24131 | | Shift along axis 150.74707237 |
| 24132 | | |
| 24133 | | |
| 24134 | | > hide #!16 models |
| 24135 | | |
| 24136 | | > hide #31.12 models |
| 24137 | | |
| 24138 | | > show #35.9 models |
| 24139 | | |
| 24140 | | > hide #35.9 models |
| 24141 | | |
| 24142 | | > show #35.9 models |
| 24143 | | |
| 24144 | | > show #!16 models |
| 24145 | | |
| 24146 | | > hide #!16 models |
| 24147 | | |
| 24148 | | > hide #35.9 models |
| 24149 | | |
| 24150 | | > hide #35.10 models |
| 24151 | | |
| 24152 | | > show #35.9 models |
| 24153 | | |
| 24154 | | > hide #39.1 models |
| 24155 | | |
| 24156 | | Drag select of 11 residues |
| 24157 | | |
| 24158 | | > select up |
| 24159 | | |
| 24160 | | 130 atoms, 130 bonds, 17 residues, 1 model selected |
| 24161 | | |
| 24162 | | > delete sel |
| 24163 | | |
| 24164 | | > show #39.1 models |
| 24165 | | |
| 24166 | | > select #35.9/L:317 |
| 24167 | | |
| 24168 | | 9 atoms, 8 bonds, 1 residue, 1 model selected |
| 24169 | | |
| 24170 | | > delete sel |
| 24171 | | |
| 24172 | | > hide #39.1 models |
| 24173 | | |
| 24174 | | Drag select of 4 residues |
| 24175 | | |
| 24176 | | > delete sel |
| 24177 | | |
| 24178 | | Drag select of 5 residues |
| 24179 | | |
| 24180 | | > delete sel |
| 24181 | | |
| 24182 | | > select #35.9/L:327 |
| 24183 | | |
| 24184 | | 8 atoms, 7 bonds, 1 residue, 1 model selected |
| 24185 | | |
| 24186 | | > delete sel |
| 24187 | | |
| 24188 | | > show #39.1 models |
| 24189 | | |
| 24190 | | > hide #39.1 models |
| 24191 | | |
| 24192 | | > select #35.9/L:328 |
| 24193 | | |
| 24194 | | 8 atoms, 7 bonds, 1 residue, 1 model selected |
| 24195 | | |
| 24196 | | > delete sel |
| 24197 | | |
| 24198 | | > select #35.9/L:329 |
| 24199 | | |
| 24200 | | 8 atoms, 7 bonds, 1 residue, 1 model selected |
| 24201 | | |
| 24202 | | > delete sel |
| 24203 | | |
| 24204 | | > show #39.1 models |
| 24205 | | |
| 24206 | | > select #35.9/L:330 |
| 24207 | | |
| 24208 | | 9 atoms, 8 bonds, 1 residue, 1 model selected |
| 24209 | | |
| 24210 | | > delete sel |
| 24211 | | |
| 24212 | | > show #!16 models |
| 24213 | | |
| 24214 | | > fitmap #35.9 inMap #16 |
| 24215 | | |
| 24216 | | Fit molecule COPI_soluble_job155_dhelix.pdb L (#35.9) to map |
| 24217 | | W20_J4026_consensus_resampled.mrc (#16) using 930 atoms |
| 24218 | | average map value = 0.3516, steps = 52 |
| 24219 | | shifted from previous position = 0.499 |
| 24220 | | rotated from previous position = 6.25 degrees |
| 24221 | | atoms outside contour = 295, contour level = 0.25963 |
| 24222 | | |
| 24223 | | Position of COPI_soluble_job155_dhelix.pdb L (#35.9) relative to |
| 24224 | | W20_J4026_consensus_resampled.mrc (#16) coordinates: |
| 24225 | | Matrix rotation and translation |
| 24226 | | -0.93642912 0.16612187 -0.30903727 462.34398677 |
| 24227 | | 0.11986381 -0.67634146 -0.72677018 585.05727704 |
| 24228 | | -0.32974714 -0.71761114 0.61343384 270.00020679 |
| 24229 | | Axis 0.17783383 0.40210728 -0.89815637 |
| 24230 | | Axis point 261.88005557 349.74957427 0.00000000 |
| 24231 | | Rotation angle (degrees) 178.52437469 |
| 24232 | | Shift along axis 74.97378580 |
| 24233 | | |
| 24234 | | |
| 24235 | | > show #35.8 models |
| 24236 | | |
| 24237 | | > hide #35.8 models |
| 24238 | | |
| 24239 | | > show #!35.11 models |
| 24240 | | |
| 24241 | | > show #31.12 models |
| 24242 | | |
| 24243 | | > show #31.11 models |
| 24244 | | |
| 24245 | | > show #31.10 models |
| 24246 | | |
| 24247 | | > show #31.9 models |
| 24248 | | |
| 24249 | | > show #31.8 models |
| 24250 | | |
| 24251 | | > show #31.7 models |
| 24252 | | |
| 24253 | | > show #31.6 models |
| 24254 | | |
| 24255 | | > show #31.5 models |
| 24256 | | |
| 24257 | | > show #!31.4 models |
| 24258 | | |
| 24259 | | > show #31.3 models |
| 24260 | | |
| 24261 | | > show #31.2 models |
| 24262 | | |
| 24263 | | > combine #31.1 #31.2 #31.3 #31.4 #31.5 #31.6 #31.7 #31.8 #31.9 #31.10 #31.11 |
| 24264 | | > #31.12 #39.1 #35.9 #35.11 |
| 24265 | | |
| 24266 | | Remapping chain ID 'A' in fold_2025_01_17_alpha1_330gamma1_330zeta_model_0.cif |
| 24267 | | A #39.1 to 'M' |
| 24268 | | Remapping chain ID 'L' in COPI_soluble_job155_dhelix.pdb L #35.9 to 'N' |
| 24269 | | Remapping chain ID 'N' in COPI_soluble_job155_dhelix.pdb N #35.11 to 'O' |
| 24270 | | |
| 24271 | | > rename #40 COPI_cyto_J4026_model |
| 24272 | | |
| 24273 | | > hide #!40 models |
| 24274 | | |
| 24275 | | > show #!40 models |
| 24276 | | |
| 24277 | | > hide #!40 models |
| 24278 | | |
| 24279 | | > hide #!16 models |
| 24280 | | |
| 24281 | | > show #!18 models |
| 24282 | | |
| 24283 | | > fitmap #35.11 inMap #18 |
| 24284 | | |
| 24285 | | Fit molecule COPI_soluble_job155_dhelix.pdb N (#35.11) to map |
| 24286 | | W20_J4027_consensus_resampled.mrc (#18) using 1064 atoms |
| 24287 | | average map value = 0.345, steps = 52 |
| 24288 | | shifted from previous position = 0.96 |
| 24289 | | rotated from previous position = 4.13 degrees |
| 24290 | | atoms outside contour = 305, contour level = 0.25259 |
| 24291 | | |
| 24292 | | Position of COPI_soluble_job155_dhelix.pdb N (#35.11) relative to |
| 24293 | | W20_J4027_consensus_resampled.mrc (#18) coordinates: |
| 24294 | | Matrix rotation and translation |
| 24295 | | -0.95797163 0.17569317 -0.22676476 434.71371371 |
| 24296 | | 0.05774245 -0.65621750 -0.75235923 607.00290226 |
| 24297 | | -0.28099138 -0.73383275 0.61849280 258.09556438 |
| 24298 | | Axis 0.14127895 0.41352064 -0.89946703 |
| 24299 | | Axis point 248.09458414 355.10604389 0.00000000 |
| 24300 | | Rotation angle (degrees) 176.24059016 |
| 24301 | | Shift along axis 80.27567222 |
| 24302 | | |
| 24303 | | |
| 24304 | | > fitmap #35.9 inMap #18 |
| 24305 | | |
| 24306 | | Fit molecule COPI_soluble_job155_dhelix.pdb L (#35.9) to map |
| 24307 | | W20_J4027_consensus_resampled.mrc (#18) using 930 atoms |
| 24308 | | average map value = 0.3717, steps = 64 |
| 24309 | | shifted from previous position = 4.09 |
| 24310 | | rotated from previous position = 12 degrees |
| 24311 | | atoms outside contour = 279, contour level = 0.25259 |
| 24312 | | |
| 24313 | | Position of COPI_soluble_job155_dhelix.pdb L (#35.9) relative to |
| 24314 | | W20_J4027_consensus_resampled.mrc (#18) coordinates: |
| 24315 | | Matrix rotation and translation |
| 24316 | | -0.98236672 0.03849512 -0.18295835 476.12367349 |
| 24317 | | 0.12352589 -0.60093803 -0.78969288 580.42963487 |
| 24318 | | -0.14034595 -0.79836809 0.58558637 250.87293860 |
| 24319 | | Axis -0.09083469 -0.44617724 0.89032293 |
| 24320 | | Axis point 243.54419066 359.36795920 0.00000000 |
| 24321 | | Rotation angle (degrees) 177.26292686 |
| 24322 | | Shift along axis -78.86510835 |
| 24323 | | |
| 24324 | | |
| 24325 | | > fitmap #39.1 inMap #18 |
| 24326 | | |
| 24327 | | Fit molecule fold_2025_01_17_alpha1_330gamma1_330zeta_model_0.cif A (#39.1) to |
| 24328 | | map W20_J4027_consensus_resampled.mrc (#18) using 2567 atoms |
| 24329 | | average map value = 0.2497, steps = 140 |
| 24330 | | shifted from previous position = 20.2 |
| 24331 | | rotated from previous position = 23.5 degrees |
| 24332 | | atoms outside contour = 1358, contour level = 0.25259 |
| 24333 | | |
| 24334 | | Position of fold_2025_01_17_alpha1_330gamma1_330zeta_model_0.cif A (#39.1) |
| 24335 | | relative to W20_J4027_consensus_resampled.mrc (#18) coordinates: |
| 24336 | | Matrix rotation and translation |
| 24337 | | 0.71011541 0.46894974 -0.52518782 133.17594101 |
| 24338 | | 0.50811821 -0.85767517 -0.07879844 140.50399717 |
| 24339 | | -0.48739306 -0.21090151 -0.84733026 102.37727978 |
| 24340 | | Axis -0.92459305 -0.26452658 0.27414118 |
| 24341 | | Axis point 0.00000000 53.88234017 68.89098797 |
| 24342 | | Rotation angle (degrees) 175.90338628 |
| 24343 | | Shift along axis -132.23476323 |
| 24344 | | |
| 24345 | | |
| 24346 | | > select add #39.1 |
| 24347 | | |
| 24348 | | 2567 atoms, 2609 bonds, 330 residues, 1 model selected |
| 24349 | | |
| 24350 | | > ui mousemode right "translate selected models" |
| 24351 | | |
| 24352 | | > view matrix models |
| 24353 | | > #39.1,0.71012,0.46895,-0.52519,127.98,0.50812,-0.85768,-0.078798,150.01,-0.48739,-0.2109,-0.84733,99.315 |
| 24354 | | |
| 24355 | | > fitmap #39.1 inMap #18 |
| 24356 | | |
| 24357 | | Fit molecule fold_2025_01_17_alpha1_330gamma1_330zeta_model_0.cif A (#39.1) to |
| 24358 | | map W20_J4027_consensus_resampled.mrc (#18) using 2567 atoms |
| 24359 | | average map value = 0.2908, steps = 104 |
| 24360 | | shifted from previous position = 3.89 |
| 24361 | | rotated from previous position = 15.2 degrees |
| 24362 | | atoms outside contour = 938, contour level = 0.25259 |
| 24363 | | |
| 24364 | | Position of fold_2025_01_17_alpha1_330gamma1_330zeta_model_0.cif A (#39.1) |
| 24365 | | relative to W20_J4027_consensus_resampled.mrc (#18) coordinates: |
| 24366 | | Matrix rotation and translation |
| 24367 | | 0.65350179 0.33145691 -0.68049373 127.41590893 |
| 24368 | | 0.52879246 -0.84317616 0.09712103 144.98236935 |
| 24369 | | -0.54158466 -0.42330872 -0.72628905 97.39971457 |
| 24370 | | Axis -0.90721165 -0.24214590 0.34399476 |
| 24371 | | Axis point 0.00000000 66.60547701 63.44217524 |
| 24372 | | Rotation angle (degrees) 163.33177472 |
| 24373 | | Shift along axis -117.19509172 |
| 24374 | | |
| 24375 | | |
| 24376 | | > hide #31.12 models |
| 24377 | | |
| 24378 | | > hide #!31 models |
| 24379 | | |
| 24380 | | > show #!32 models |
| 24381 | | |
| 24382 | | > hide #32.13 models |
| 24383 | | |
| 24384 | | > fitmap #32.12 inMap #18 |
| 24385 | | |
| 24386 | | Fit molecule COPI_cyto_J4027_model L (#32.12) to map |
| 24387 | | W20_J4027_consensus_resampled.mrc (#18) using 1420 atoms |
| 24388 | | average map value = 0.249, steps = 84 |
| 24389 | | shifted from previous position = 0.209 |
| 24390 | | rotated from previous position = 0.257 degrees |
| 24391 | | atoms outside contour = 664, contour level = 0.25259 |
| 24392 | | |
| 24393 | | Position of COPI_cyto_J4027_model L (#32.12) relative to |
| 24394 | | W20_J4027_consensus_resampled.mrc (#18) coordinates: |
| 24395 | | Matrix rotation and translation |
| 24396 | | -0.36763985 -0.91576410 -0.16191685 275.33631018 |
| 24397 | | -0.79632880 0.22007538 0.56340686 164.04444754 |
| 24398 | | -0.48031386 0.33606986 -0.81015780 242.91724000 |
| 24399 | | Axis -0.55576549 0.77837779 0.29198072 |
| 24400 | | Axis point 211.47624167 0.00000000 70.72859152 |
| 24401 | | Rotation angle (degrees) 168.19824647 |
| 24402 | | Shift along axis 45.59328469 |
| 24403 | | |
| 24404 | | |
| 24405 | | > view matrix models |
| 24406 | | > #39.1,0.6535,0.33146,-0.68049,127.38,0.52879,-0.84318,0.097121,144.98,-0.54158,-0.42331,-0.72629,97.387 |
| 24407 | | |
| 24408 | | > select subtract #39.1 |
| 24409 | | |
| 24410 | | Nothing selected |
| 24411 | | |
| 24412 | | > fitmap #32.5 inMap #18 |
| 24413 | | |
| 24414 | | Fit molecule COPI_cyto_J4027_model E (#32.5) to map |
| 24415 | | W20_J4027_consensus_resampled.mrc (#18) using 1876 atoms |
| 24416 | | average map value = 0.3307, steps = 28 |
| 24417 | | shifted from previous position = 0.0226 |
| 24418 | | rotated from previous position = 0.025 degrees |
| 24419 | | atoms outside contour = 588, contour level = 0.25259 |
| 24420 | | |
| 24421 | | Position of COPI_cyto_J4027_model E (#32.5) relative to |
| 24422 | | W20_J4027_consensus_resampled.mrc (#18) coordinates: |
| 24423 | | Matrix rotation and translation |
| 24424 | | -0.38917297 -0.90711538 -0.16026883 277.45394634 |
| 24425 | | -0.76472866 0.22115752 0.60521025 157.23067335 |
| 24426 | | -0.51355087 0.35809364 -0.77976565 242.80657971 |
| 24427 | | Axis -0.54426642 0.77809236 0.31360219 |
| 24428 | | Axis point 210.56184964 0.00000000 67.30315702 |
| 24429 | | Rotation angle (degrees) 166.87842423 |
| 24430 | | Shift along axis 47.47579529 |
| 24431 | | |
| 24432 | | |
| 24433 | | > combine #32.1 #32.2 #32.3 #32.4 #32.5 #32.6 #32.7 #32.8 #32.9 #32.10 #32.11 |
| 24434 | | > #32.12 #39.1 #35.9 #35.11 |
| 24435 | | |
| 24436 | | Remapping chain ID 'A' in fold_2025_01_17_alpha1_330gamma1_330zeta_model_0.cif |
| 24437 | | A #39.1 to 'M' |
| 24438 | | Remapping chain ID 'L' in COPI_soluble_job155_dhelix.pdb L #35.9 to 'N' |
| 24439 | | Remapping chain ID 'N' in COPI_soluble_job155_dhelix.pdb N #35.11 to 'O' |
| 24440 | | |
| 24441 | | > rename #41 COPI_cyto_J4017_model |
| 24442 | | |
| 24443 | | > hide #39.1 models |
| 24444 | | |
| 24445 | | > show #39.1 models |
| 24446 | | |
| 24447 | | > hide #!41 models |
| 24448 | | |
| 24449 | | > hide #!32 models |
| 24450 | | |
| 24451 | | > hide #!18 models |
| 24452 | | |
| 24453 | | > show #!20 models |
| 24454 | | |
| 24455 | | > fitmap #35.9 inMap #10 |
| 24456 | | |
| 24457 | | Fit molecule COPI_soluble_job155_dhelix.pdb L (#35.9) to map |
| 24458 | | W20_J4023_consensus_resampled.mrc (#10) using 930 atoms |
| 24459 | | average map value = 0.2393, steps = 52 |
| 24460 | | shifted from previous position = 2.69 |
| 24461 | | rotated from previous position = 6.1 degrees |
| 24462 | | atoms outside contour = 585, contour level = 0.259 |
| 24463 | | |
| 24464 | | Position of COPI_soluble_job155_dhelix.pdb L (#35.9) relative to |
| 24465 | | W20_J4023_consensus_resampled.mrc (#10) coordinates: |
| 24466 | | Matrix rotation and translation |
| 24467 | | -0.98125869 0.10123862 -0.16395770 446.50000833 |
| 24468 | | 0.05661840 -0.66183176 -0.74751126 606.96394850 |
| 24469 | | -0.18418942 -0.74278494 0.64369619 224.44341669 |
| 24470 | | Axis 0.09602403 0.41104534 -0.90654350 |
| 24471 | | Axis point 239.00882222 351.39277051 0.00000000 |
| 24472 | | Rotation angle (degrees) 178.58980379 |
| 24473 | | Shift along axis 88.89671334 |
| 24474 | | |
| 24475 | | |
| 24476 | | > fitmap #35.11 inMap #10 |
| 24477 | | |
| 24478 | | Fit molecule COPI_soluble_job155_dhelix.pdb N (#35.11) to map |
| 24479 | | W20_J4023_consensus_resampled.mrc (#10) using 1064 atoms |
| 24480 | | average map value = 0.2705, steps = 48 |
| 24481 | | shifted from previous position = 1.17 |
| 24482 | | rotated from previous position = 3.69 degrees |
| 24483 | | atoms outside contour = 361, contour level = 0.259 |
| 24484 | | |
| 24485 | | Position of COPI_soluble_job155_dhelix.pdb N (#35.11) relative to |
| 24486 | | W20_J4023_consensus_resampled.mrc (#10) coordinates: |
| 24487 | | Matrix rotation and translation |
| 24488 | | -0.95447536 0.16472623 -0.24868064 445.72556094 |
| 24489 | | 0.09748552 -0.61564640 -0.78196936 592.79673482 |
| 24490 | | -0.28191020 -0.77061325 0.57156090 289.62220826 |
| 24491 | | Axis 0.14970170 0.43804800 -0.88639909 |
| 24492 | | Axis point 253.33667423 363.30405703 0.00000000 |
| 24493 | | Rotation angle (degrees) 177.82629912 |
| 24494 | | Shift along axis 69.67843860 |
| 24495 | | |
| 24496 | | |
| 24497 | | > fitmap #39.1 inMap #10 |
| 24498 | | |
| 24499 | | Fit molecule fold_2025_01_17_alpha1_330gamma1_330zeta_model_0.cif A (#39.1) to |
| 24500 | | map W20_J4023_consensus_resampled.mrc (#10) using 2567 atoms |
| 24501 | | average map value = 0.197, steps = 180 |
| 24502 | | shifted from previous position = 18.8 |
| 24503 | | rotated from previous position = 39.4 degrees |
| 24504 | | atoms outside contour = 2326, contour level = 0.259 |
| 24505 | | |
| 24506 | | Position of fold_2025_01_17_alpha1_330gamma1_330zeta_model_0.cif A (#39.1) |
| 24507 | | relative to W20_J4023_consensus_resampled.mrc (#10) coordinates: |
| 24508 | | Matrix rotation and translation |
| 24509 | | 0.77937618 0.53787625 -0.32134390 138.00151844 |
| 24510 | | 0.38455520 -0.81555599 -0.43241846 137.27793171 |
| 24511 | | -0.49466156 0.21344218 -0.84246803 102.09006947 |
| 24512 | | Axis 0.94140147 0.25262649 -0.22347958 |
| 24513 | | Axis point 0.00000000 37.55487421 76.95697173 |
| 24514 | | Rotation angle (degrees) 159.93832539 |
| 24515 | | Shift along axis 141.77982886 |
| 24516 | | |
| 24517 | | |
| 24518 | | > select add #39.1 |
| 24519 | | |
| 24520 | | 2567 atoms, 2609 bonds, 330 residues, 1 model selected |
| 24521 | | |
| 24522 | | > view matrix models |
| 24523 | | > #39.1,0.77938,0.53788,-0.32134,133.03,0.38456,-0.81556,-0.43242,136.21,-0.49466,0.21344,-0.84247,87.787 |
| 24524 | | |
| 24525 | | > fitmap #39.1 inMap #10 |
| 24526 | | |
| 24527 | | Fit molecule fold_2025_01_17_alpha1_330gamma1_330zeta_model_0.cif A (#39.1) to |
| 24528 | | map W20_J4023_consensus_resampled.mrc (#10) using 2567 atoms |
| 24529 | | average map value = 0.197, steps = 148 |
| 24530 | | shifted from previous position = 15.2 |
| 24531 | | rotated from previous position = 0.0116 degrees |
| 24532 | | atoms outside contour = 2326, contour level = 0.259 |
| 24533 | | |
| 24534 | | Position of fold_2025_01_17_alpha1_330gamma1_330zeta_model_0.cif A (#39.1) |
| 24535 | | relative to W20_J4023_consensus_resampled.mrc (#10) coordinates: |
| 24536 | | Matrix rotation and translation |
| 24537 | | 0.77933517 0.53786894 -0.32145559 138.00257192 |
| 24538 | | 0.38460571 -0.81561140 -0.43226900 137.27863469 |
| 24539 | | -0.49468691 0.21324878 -0.84250212 102.09071968 |
| 24540 | | Axis 0.94139228 0.25263228 -0.22351177 |
| 24541 | | Axis point 0.00000000 37.56089500 76.95480112 |
| 24542 | | Rotation angle (degrees) 159.94922815 |
| 24543 | | Shift along axis 141.77709241 |
| 24544 | | |
| 24545 | | |
| 24546 | | > view matrix models |
| 24547 | | > #39.1,0.77934,0.53787,-0.32146,130.24,0.38461,-0.81561,-0.43227,141.73,-0.49469,0.21325,-0.8425,88.079 |
| 24548 | | |
| 24549 | | > view matrix models |
| 24550 | | > #39.1,0.77934,0.53787,-0.32146,135.31,0.38461,-0.81561,-0.43227,140.11,-0.49469,0.21325,-0.8425,84.463 |
| 24551 | | |
| 24552 | | > fitmap #39.1 inMap #10 |
| 24553 | | |
| 24554 | | Fit molecule fold_2025_01_17_alpha1_330gamma1_330zeta_model_0.cif A (#39.1) to |
| 24555 | | map W20_J4023_consensus_resampled.mrc (#10) using 2567 atoms |
| 24556 | | average map value = 0.197, steps = 172 |
| 24557 | | shifted from previous position = 18.1 |
| 24558 | | rotated from previous position = 0.0154 degrees |
| 24559 | | atoms outside contour = 2326, contour level = 0.259 |
| 24560 | | |
| 24561 | | Position of fold_2025_01_17_alpha1_330gamma1_330zeta_model_0.cif A (#39.1) |
| 24562 | | relative to W20_J4023_consensus_resampled.mrc (#10) coordinates: |
| 24563 | | Matrix rotation and translation |
| 24564 | | 0.77924517 0.53791820 -0.32159133 138.00026966 |
| 24565 | | 0.38472109 -0.81564702 -0.43209909 137.28065889 |
| 24566 | | -0.49473897 0.21298817 -0.84253747 102.08755822 |
| 24567 | | Axis 0.94137014 0.25267283 -0.22355918 |
| 24568 | | Axis point 0.00000000 37.56685145 76.95023483 |
| 24569 | | Rotation angle (degrees) 159.96268257 |
| 24570 | | Shift along axis 141.77381369 |
| 24571 | | |
| 24572 | | |
| 24573 | | > view matrix models |
| 24574 | | > #39.1,0.77925,0.53792,-0.32159,128.09,0.38472,-0.81565,-0.4321,135.26,-0.49474,0.21299,-0.84254,82.235 |
| 24575 | | |
| 24576 | | > view matrix models |
| 24577 | | > #39.1,0.77925,0.53792,-0.32159,135.47,0.38472,-0.81565,-0.4321,136.17,-0.49474,0.21299,-0.84254,76.831 |
| 24578 | | |
| 24579 | | > view matrix models |
| 24580 | | > #39.1,0.77925,0.53792,-0.32159,139.06,0.38472,-0.81565,-0.4321,133.92,-0.49474,0.21299,-0.84254,83.878 |
| 24581 | | |
| 24582 | | > view matrix models |
| 24583 | | > #39.1,0.77925,0.53792,-0.32159,133.59,0.38472,-0.81565,-0.4321,136.36,-0.49474,0.21299,-0.84254,86.161 |
| 24584 | | |
| 24585 | | > view matrix models |
| 24586 | | > #39.1,0.77925,0.53792,-0.32159,137.64,0.38472,-0.81565,-0.4321,140.68,-0.49474,0.21299,-0.84254,88.092 |
| 24587 | | |
| 24588 | | > ui mousemode right "rotate selected models" |
| 24589 | | |
| 24590 | | > view matrix models |
| 24591 | | > #39.1,0.88557,0.46445,-0.0069167,134.98,0.45496,-0.87029,-0.18867,138.57,-0.093649,0.16394,-0.98202,91.009 |
| 24592 | | |
| 24593 | | > fitmap #39.1 inMap #10 |
| 24594 | | |
| 24595 | | Fit molecule fold_2025_01_17_alpha1_330gamma1_330zeta_model_0.cif A (#39.1) to |
| 24596 | | map W20_J4023_consensus_resampled.mrc (#10) using 2567 atoms |
| 24597 | | average map value = 0.197, steps = 144 |
| 24598 | | shifted from previous position = 19.4 |
| 24599 | | rotated from previous position = 24.7 degrees |
| 24600 | | atoms outside contour = 2327, contour level = 0.259 |
| 24601 | | |
| 24602 | | Position of fold_2025_01_17_alpha1_330gamma1_330zeta_model_0.cif A (#39.1) |
| 24603 | | relative to W20_J4023_consensus_resampled.mrc (#10) coordinates: |
| 24604 | | Matrix rotation and translation |
| 24605 | | 0.77925174 0.53807988 -0.32130481 137.99460111 |
| 24606 | | 0.38488292 -0.81549555 -0.43224086 137.29386764 |
| 24607 | | -0.49460275 0.21315971 -0.84257407 102.10795439 |
| 24608 | | Axis 0.94137004 0.25276936 -0.22345043 |
| 24609 | | Axis point 0.00000000 37.55980635 76.95624722 |
| 24610 | | Rotation angle (degrees) 159.95253140 |
| 24611 | | Shift along axis 141.79160050 |
| 24612 | | |
| 24613 | | |
| 24614 | | > view matrix models |
| 24615 | | > #39.1,0.68606,0.65344,-0.3199,137.9,0.57062,-0.75605,-0.32059,137.24,-0.45135,0.037399,-0.89156,102.26 |
| 24616 | | |
| 24617 | | > ui mousemode right "translate selected models" |
| 24618 | | |
| 24619 | | > view matrix models |
| 24620 | | > #39.1,0.68606,0.65344,-0.3199,131.56,0.57062,-0.75605,-0.32059,145.97,-0.45135,0.037399,-0.89156,83.123 |
| 24621 | | |
| 24622 | | > view matrix models |
| 24623 | | > #39.1,0.68606,0.65344,-0.3199,134.43,0.57062,-0.75605,-0.32059,139.6,-0.45135,0.037399,-0.89156,83.125 |
| 24624 | | |
| 24625 | | > view matrix models |
| 24626 | | > #39.1,0.68606,0.65344,-0.3199,130.69,0.57062,-0.75605,-0.32059,139.63,-0.45135,0.037399,-0.89156,81.892 |
| 24627 | | |
| 24628 | | > view matrix models |
| 24629 | | > #39.1,0.68606,0.65344,-0.3199,132.35,0.57062,-0.75605,-0.32059,136.91,-0.45135,0.037399,-0.89156,85.265 |
| 24630 | | |
| 24631 | | > view matrix models |
| 24632 | | > #39.1,0.68606,0.65344,-0.3199,127.51,0.57062,-0.75605,-0.32059,139.3,-0.45135,0.037399,-0.89156,87.993 |
| 24633 | | |
| 24634 | | > view matrix models |
| 24635 | | > #39.1,0.68606,0.65344,-0.3199,131.61,0.57062,-0.75605,-0.32059,143.53,-0.45135,0.037399,-0.89156,89.59 |
| 24636 | | |
| 24637 | | > fitmap #39.1 inMap #10 |
| 24638 | | |
| 24639 | | Fit molecule fold_2025_01_17_alpha1_330gamma1_330zeta_model_0.cif A (#39.1) to |
| 24640 | | map W20_J4023_consensus_resampled.mrc (#10) using 2567 atoms |
| 24641 | | average map value = 0.197, steps = 144 |
| 24642 | | shifted from previous position = 14.5 |
| 24643 | | rotated from previous position = 13.3 degrees |
| 24644 | | atoms outside contour = 2327, contour level = 0.259 |
| 24645 | | |
| 24646 | | Position of fold_2025_01_17_alpha1_330gamma1_330zeta_model_0.cif A (#39.1) |
| 24647 | | relative to W20_J4023_consensus_resampled.mrc (#10) coordinates: |
| 24648 | | Matrix rotation and translation |
| 24649 | | 0.77919349 0.53804611 -0.32150254 137.99342007 |
| 24650 | | 0.38489409 -0.81557949 -0.43207249 137.29343954 |
| 24651 | | -0.49468580 0.21292364 -0.84258500 102.10246297 |
| 24652 | | Axis 0.94135648 0.25275684 -0.22352173 |
| 24653 | | Axis point 0.00000000 37.56820104 76.95313719 |
| 24654 | | Rotation angle (degrees) 159.96533135 |
| 24655 | | Shift along axis 141.78073688 |
| 24656 | | |
| 24657 | | |
| 24658 | | > select subtract #39.1 |
| 24659 | | |
| 24660 | | Nothing selected |
| 24661 | | |
| 24662 | | > hide #39.1 models |
| 24663 | | |
| 24664 | | > show #!33 models |
| 24665 | | |
| 24666 | | > hide #33.1 models |
| 24667 | | |
| 24668 | | > hide #33.2 models |
| 24669 | | |
| 24670 | | > hide #33.3 models |
| 24671 | | |
| 24672 | | > hide #!33.4 models |
| 24673 | | |
| 24674 | | > hide #33.5 models |
| 24675 | | |
| 24676 | | > hide #33.6 models |
| 24677 | | |
| 24678 | | > hide #33.7 models |
| 24679 | | |
| 24680 | | > hide #33.8 models |
| 24681 | | |
| 24682 | | > hide #33.9 models |
| 24683 | | |
| 24684 | | > hide #33.10 models |
| 24685 | | |
| 24686 | | > hide #33.11 models |
| 24687 | | |
| 24688 | | > hide #33.12 models |
| 24689 | | |
| 24690 | | > fitmap #33.13 inMap #10 |
| 24691 | | |
| 24692 | | Fit molecule COPI_cyto_J4028_model M (#33.13) to map |
| 24693 | | W20_J4023_consensus_resampled.mrc (#10) using 4552 atoms |
| 24694 | | average map value = 0.2144, steps = 128 |
| 24695 | | shifted from previous position = 5.65 |
| 24696 | | rotated from previous position = 7.59 degrees |
| 24697 | | atoms outside contour = 3456, contour level = 0.259 |
| 24698 | | |
| 24699 | | Position of COPI_cyto_J4028_model M (#33.13) relative to |
| 24700 | | W20_J4023_consensus_resampled.mrc (#10) coordinates: |
| 24701 | | Matrix rotation and translation |
| 24702 | | -0.35039846 -0.93657228 -0.00730075 262.48644706 |
| 24703 | | -0.86127456 0.31914510 0.39541441 178.27483489 |
| 24704 | | -0.36800418 0.14484055 -0.91847382 254.98975169 |
| 24705 | | Axis -0.56232261 0.80946867 0.16897857 |
| 24706 | | Axis point 210.46623440 0.00000000 89.03589265 |
| 24707 | | Rotation angle (degrees) 167.12630425 |
| 24708 | | Shift along axis 39.79363149 |
| 24709 | | |
| 24710 | | |
| 24711 | | > hide #!20 models |
| 24712 | | |
| 24713 | | > hide #!35.11 models |
| 24714 | | |
| 24715 | | > show #!35.11 models |
| 24716 | | |
| 24717 | | > hide #!35.11 models |
| 24718 | | |
| 24719 | | > hide #35.9 models |
| 24720 | | |
| 24721 | | > show #35.9 models |
| 24722 | | |
| 24723 | | > hide #33.13 models |
| 24724 | | |
| 24725 | | > hide #35.9 models |
| 24726 | | |
| 24727 | | > show #33.13 models |
| 24728 | | |
| 24729 | | > ui mousemode right select |
| 24730 | | |
| 24731 | | Drag select of 242 residues |
| 24732 | | |
| 24733 | | > select up |
| 24734 | | |
| 24735 | | 2060 atoms, 2089 bonds, 262 residues, 1 model selected |
| 24736 | | |
| 24737 | | > select clear |
| 24738 | | |
| 24739 | | Drag select of 128 residues |
| 24740 | | |
| 24741 | | > select up |
| 24742 | | |
| 24743 | | 1192 atoms, 1211 bonds, 151 residues, 1 model selected |
| 24744 | | |
| 24745 | | > delete sel |
| 24746 | | |
| 24747 | | > select clear |
| 24748 | | |
| 24749 | | Drag select of 5 residues |
| 24750 | | Drag select of 16 residues |
| 24751 | | Drag select of 64 residues, 1 pseudobonds |
| 24752 | | |
| 24753 | | > select up |
| 24754 | | |
| 24755 | | 587 atoms, 592 bonds, 1 pseudobond, 73 residues, 2 models selected |
| 24756 | | |
| 24757 | | > delete sel |
| 24758 | | |
| 24759 | | Drag select of 4 residues, 1 pseudobonds |
| 24760 | | |
| 24761 | | > delete sel |
| 24762 | | |
| 24763 | | Drag select of 1 pseudobonds |
| 24764 | | |
| 24765 | | > delete sel |
| 24766 | | |
| 24767 | | > select add #33.13 |
| 24768 | | |
| 24769 | | 2744 atoms, 2786 bonds, 1 pseudobond, 354 residues, 2 models selected |
| 24770 | | |
| 24771 | | > select subtract #33.13 |
| 24772 | | |
| 24773 | | Nothing selected |
| 24774 | | |
| 24775 | | > combine #30.13 |
| 24776 | | |
| 24777 | | > hide #!33.13 models |
| 24778 | | |
| 24779 | | Drag select of 172 residues |
| 24780 | | |
| 24781 | | > select up |
| 24782 | | |
| 24783 | | 1391 atoms, 1413 bonds, 176 residues, 1 model selected |
| 24784 | | |
| 24785 | | > delete sel |
| 24786 | | |
| 24787 | | Drag select of 24 residues |
| 24788 | | |
| 24789 | | > select up |
| 24790 | | |
| 24791 | | 388 atoms, 392 bonds, 48 residues, 1 model selected |
| 24792 | | |
| 24793 | | > delete sel |
| 24794 | | |
| 24795 | | Drag select of 15 residues, 1 pseudobonds |
| 24796 | | |
| 24797 | | > select up |
| 24798 | | |
| 24799 | | 269 atoms, 268 bonds, 1 pseudobond, 37 residues, 2 models selected |
| 24800 | | |
| 24801 | | > select clear |
| 24802 | | |
| 24803 | | Drag select of 9 residues, 1 pseudobonds |
| 24804 | | |
| 24805 | | > select up |
| 24806 | | |
| 24807 | | 155 atoms, 154 bonds, 1 pseudobond, 21 residues, 2 models selected |
| 24808 | | |
| 24809 | | > delete sel |
| 24810 | | |
| 24811 | | Drag select of 7 residues |
| 24812 | | |
| 24813 | | > select up |
| 24814 | | |
| 24815 | | 136 atoms, 136 bonds, 19 residues, 1 model selected |
| 24816 | | |
| 24817 | | > select clear |
| 24818 | | |
| 24819 | | > fitmap #42 inMap #20 |
| 24820 | | |
| 24821 | | Fit molecule copy of COPI_cyto_J4025_model M (#42) to map |
| 24822 | | W20_J4028_consensus_resampled.mrc (#20) using 2618 atoms |
| 24823 | | average map value = 0.306, steps = 180 |
| 24824 | | shifted from previous position = 23.6 |
| 24825 | | rotated from previous position = 33.1 degrees |
| 24826 | | atoms outside contour = 931, contour level = 0.25474 |
| 24827 | | |
| 24828 | | Position of copy of COPI_cyto_J4025_model M (#42) relative to |
| 24829 | | W20_J4028_consensus_resampled.mrc (#20) coordinates: |
| 24830 | | Matrix rotation and translation |
| 24831 | | -0.14963361 -0.97522083 0.16295437 216.33302484 |
| 24832 | | -0.96633931 0.17912766 0.18466631 233.37303521 |
| 24833 | | -0.20928006 -0.12983692 -0.96919773 254.43371461 |
| 24834 | | Axis -0.64527959 0.76372913 0.01822260 |
| 24835 | | Axis point 227.29901935 0.00000000 104.64971477 |
| 24836 | | Rotation angle (degrees) 165.89525591 |
| 24837 | | Shift along axis 43.27494515 |
| 24838 | | |
| 24839 | | |
| 24840 | | > show #!20 models |
| 24841 | | |
| 24842 | | > show #35.9 models |
| 24843 | | |
| 24844 | | > hide #35.9 models |
| 24845 | | |
| 24846 | | > show #35.9 models |
| 24847 | | |
| 24848 | | > fitmap #35.9 inMap #20 |
| 24849 | | |
| 24850 | | Fit molecule COPI_soluble_job155_dhelix.pdb L (#35.9) to map |
| 24851 | | W20_J4028_consensus_resampled.mrc (#20) using 930 atoms |
| 24852 | | average map value = 0.3762, steps = 56 |
| 24853 | | shifted from previous position = 2.58 |
| 24854 | | rotated from previous position = 5.97 degrees |
| 24855 | | atoms outside contour = 300, contour level = 0.25474 |
| 24856 | | |
| 24857 | | Position of COPI_soluble_job155_dhelix.pdb L (#35.9) relative to |
| 24858 | | W20_J4028_consensus_resampled.mrc (#20) coordinates: |
| 24859 | | Matrix rotation and translation |
| 24860 | | -0.95707237 0.18100601 -0.22638311 433.92940718 |
| 24861 | | 0.05310912 -0.65830260 -0.75087756 607.96749602 |
| 24862 | | -0.28494194 -0.73066717 0.62043016 258.74353581 |
| 24863 | | Axis 0.14221667 0.41206734 -0.89998607 |
| 24864 | | Axis point 248.77691524 354.86529873 0.00000000 |
| 24865 | | Rotation angle (degrees) 175.92541914 |
| 24866 | | Shift along axis 79.36996533 |
| 24867 | | |
| 24868 | | |
| 24869 | | > show #35.8 models |
| 24870 | | |
| 24871 | | > hide #35.8 models |
| 24872 | | |
| 24873 | | > show #!35.11 models |
| 24874 | | |
| 24875 | | > fitmap #35.11 inMap #20 |
| 24876 | | |
| 24877 | | Fit molecule COPI_soluble_job155_dhelix.pdb N (#35.11) to map |
| 24878 | | W20_J4028_consensus_resampled.mrc (#20) using 1064 atoms |
| 24879 | | average map value = 0.3378, steps = 88 |
| 24880 | | shifted from previous position = 0.716 |
| 24881 | | rotated from previous position = 7.18 degrees |
| 24882 | | atoms outside contour = 284, contour level = 0.25474 |
| 24883 | | |
| 24884 | | Position of COPI_soluble_job155_dhelix.pdb N (#35.11) relative to |
| 24885 | | W20_J4028_consensus_resampled.mrc (#20) coordinates: |
| 24886 | | Matrix rotation and translation |
| 24887 | | -0.94111577 0.21871586 -0.25780707 426.29452223 |
| 24888 | | 0.03665885 -0.69203983 -0.72092788 614.47706636 |
| 24889 | | -0.33609112 -0.68792750 0.64327172 250.03704434 |
| 24890 | | Axis 0.16425992 0.38966017 -0.90619183 |
| 24891 | | Axis point 251.98330593 349.53072602 0.00000000 |
| 24892 | | Rotation angle (degrees) 174.23481815 |
| 24893 | | Shift along axis 82.87881842 |
| 24894 | | |
| 24895 | | |
| 24896 | | > hide #!20 models |
| 24897 | | |
| 24898 | | > show #!20 models |
| 24899 | | |
| 24900 | | > hide #!20 models |
| 24901 | | |
| 24902 | | > select #35.9/L:331 |
| 24903 | | |
| 24904 | | 8 atoms, 7 bonds, 1 residue, 1 model selected |
| 24905 | | |
| 24906 | | > show #!20 models |
| 24907 | | |
| 24908 | | > hide #!20 models |
| 24909 | | |
| 24910 | | > delete sel |
| 24911 | | |
| 24912 | | > select #35.9/L:332 |
| 24913 | | |
| 24914 | | 8 atoms, 7 bonds, 1 residue, 1 model selected |
| 24915 | | |
| 24916 | | > delete sel |
| 24917 | | |
| 24918 | | > select #35.9/L:333 |
| 24919 | | |
| 24920 | | 7 atoms, 6 bonds, 1 residue, 1 model selected |
| 24921 | | |
| 24922 | | > delete sel |
| 24923 | | |
| 24924 | | > select #35.9/L:334 |
| 24925 | | |
| 24926 | | 7 atoms, 6 bonds, 1 residue, 1 model selected |
| 24927 | | |
| 24928 | | > delete sel |
| 24929 | | |
| 24930 | | > select #35.9/L:335 |
| 24931 | | |
| 24932 | | 8 atoms, 7 bonds, 1 residue, 1 model selected |
| 24933 | | |
| 24934 | | > delete sel |
| 24935 | | |
| 24936 | | > select #35.9/L:336 |
| 24937 | | |
| 24938 | | 6 atoms, 5 bonds, 1 residue, 1 model selected |
| 24939 | | |
| 24940 | | > delete sel |
| 24941 | | |
| 24942 | | > select #35.9/L:337 |
| 24943 | | |
| 24944 | | 8 atoms, 7 bonds, 1 residue, 1 model selected |
| 24945 | | |
| 24946 | | > delete sel |
| 24947 | | |
| 24948 | | > fitmap #35.9 inMap #20 |
| 24949 | | |
| 24950 | | Fit molecule COPI_soluble_job155_dhelix.pdb L (#35.9) to map |
| 24951 | | W20_J4028_consensus_resampled.mrc (#20) using 878 atoms |
| 24952 | | average map value = 0.3829, steps = 44 |
| 24953 | | shifted from previous position = 0.138 |
| 24954 | | rotated from previous position = 1.65 degrees |
| 24955 | | atoms outside contour = 274, contour level = 0.25474 |
| 24956 | | |
| 24957 | | Position of COPI_soluble_job155_dhelix.pdb L (#35.9) relative to |
| 24958 | | W20_J4028_consensus_resampled.mrc (#20) coordinates: |
| 24959 | | Matrix rotation and translation |
| 24960 | | -0.96329901 0.17783180 -0.20107426 427.85994614 |
| 24961 | | 0.03340558 -0.66383109 -0.74713610 614.70583444 |
| 24962 | | -0.26634390 -0.72643247 0.63352726 246.72265827 |
| 24963 | | Axis 0.12953006 0.40835260 -0.90358736 |
| 24964 | | Axis point 244.47252749 353.86664751 0.00000000 |
| 24965 | | Rotation angle (degrees) 175.41613396 |
| 24966 | | Shift along axis 83.50197716 |
| 24967 | | |
| 24968 | | |
| 24969 | | > show #!20 models |
| 24970 | | |
| 24971 | | > show #33.1 models |
| 24972 | | |
| 24973 | | > show #33.2 models |
| 24974 | | |
| 24975 | | > show #33.3 models |
| 24976 | | |
| 24977 | | > show #!33.4 models |
| 24978 | | |
| 24979 | | > show #33.5 models |
| 24980 | | |
| 24981 | | > show #33.6 models |
| 24982 | | |
| 24983 | | > show #33.7 models |
| 24984 | | |
| 24985 | | > show #33.8 models |
| 24986 | | |
| 24987 | | > show #33.9 models |
| 24988 | | |
| 24989 | | > show #33.10 models |
| 24990 | | |
| 24991 | | > show #33.11 models |
| 24992 | | |
| 24993 | | > show #33.12 models |
| 24994 | | |
| 24995 | | > combine #33.1 #33.2 #33.3 #33.4 #33.5 #33.6 #33.7 #33.8 #33.9 #33.10 #33.11 |
| 24996 | | > #33.12 #41 #35.9 #35.11 |
| 24997 | | |
| 24998 | | Remapping chain ID 'A' in COPI_cyto_J4017_model #41 to 'P' |
| 24999 | | Remapping chain ID 'B' in COPI_cyto_J4017_model #41 to 'Q' |
| 25000 | | Remapping chain ID 'C' in COPI_cyto_J4017_model #41 to 'R' |
| 25001 | | Remapping chain ID 'D' in COPI_cyto_J4017_model #41 to 'S' |
| 25002 | | Remapping chain ID 'E' in COPI_cyto_J4017_model #41 to 'T' |
| 25003 | | Remapping chain ID 'F' in COPI_cyto_J4017_model #41 to 'U' |
| 25004 | | Remapping chain ID 'G' in COPI_cyto_J4017_model #41 to 'V' |
| 25005 | | Remapping chain ID 'H' in COPI_cyto_J4017_model #41 to 'W' |
| 25006 | | Remapping chain ID 'I' in COPI_cyto_J4017_model #41 to 'X' |
| 25007 | | Remapping chain ID 'J' in COPI_cyto_J4017_model #41 to 'Y' |
| 25008 | | Remapping chain ID 'K' in COPI_cyto_J4017_model #41 to 'Z' |
| 25009 | | Remapping chain ID 'L' in COPI_cyto_J4017_model #41 to 'a' |
| 25010 | | Remapping chain ID 'L' in COPI_soluble_job155_dhelix.pdb L #35.9 to 'b' |
| 25011 | | Remapping chain ID 'N' in COPI_soluble_job155_dhelix.pdb N #35.11 to 'c' |
| 25012 | | |
| 25013 | | > rename #41 COPI_cyto_J4027_model |
| 25014 | | |
| 25015 | | > rename #43 COPI_cyto_J4028_model |
| 25016 | | |
| 25017 | | > hide #!20 models |
| 25018 | | |
| 25019 | | > show #!22 models |
| 25020 | | |
| 25021 | | > hide #!43 models |
| 25022 | | |
| 25023 | | > show #!43 models |
| 25024 | | |
| 25025 | | > hide #!22 models |
| 25026 | | |
| 25027 | | > show #!20 models |
| 25028 | | |
| 25029 | | > hide #!20 models |
| 25030 | | |
| 25031 | | > hide #!43 models |
| 25032 | | |
| 25033 | | > hide #!42 models |
| 25034 | | |
| 25035 | | > hide #!33 models |
| 25036 | | |
| 25037 | | > show #!16 models |
| 25038 | | |
| 25039 | | > hide #35.9 models |
| 25040 | | |
| 25041 | | > show #35.9 models |
| 25042 | | |
| 25043 | | > hide #!35 models |
| 25044 | | |
| 25045 | | > show #!40 models |
| 25046 | | |
| 25047 | | > hide #!40 models |
| 25048 | | |
| 25049 | | > hide #!16 models |
| 25050 | | |
| 25051 | | > show #!18 models |
| 25052 | | |
| 25053 | | > show #!41 models |
| 25054 | | |
| 25055 | | > hide #!41 models |
| 25056 | | |
| 25057 | | > show #!43 models |
| 25058 | | |
| 25059 | | > hide #!18 models |
| 25060 | | |
| 25061 | | > show #!20 models |
| 25062 | | |
| 25063 | | > close #43 |
| 25064 | | |
| 25065 | | > show #!42 models |
| 25066 | | |
| 25067 | | > show #!35 models |
| 25068 | | |
| 25069 | | > show #!33 models |
| 25070 | | |
| 25071 | | > combine #33.1 #33.2 #33.3 #33.4 #33.5 #33.6 #33.7 #33.8 #33.9 #33.10 #33.11 |
| 25072 | | > #33.12 #42 #35.9 #35.11 |
| 25073 | | |
| 25074 | | Remapping chain ID 'L' in COPI_soluble_job155_dhelix.pdb L #35.9 to 'N' |
| 25075 | | Remapping chain ID 'N' in COPI_soluble_job155_dhelix.pdb N #35.11 to 'O' |
| 25076 | | |
| 25077 | | > rename #43 COPI_cyto_J4028_model |
| 25078 | | |
| 25079 | | > hide #!43 models |
| 25080 | | |
| 25081 | | > show #!43 models |
| 25082 | | |
| 25083 | | > hide #!42 models |
| 25084 | | |
| 25085 | | > hide #!35 models |
| 25086 | | |
| 25087 | | > hide #!33 models |
| 25088 | | |
| 25089 | | > hide #!43 models |
| 25090 | | |
| 25091 | | > show #!43 models |
| 25092 | | |
| 25093 | | > hide #!43 models |
| 25094 | | |
| 25095 | | > hide #!20 models |
| 25096 | | |
| 25097 | | > show #!22 models |
| 25098 | | |
| 25099 | | > show #!34 models |
| 25100 | | |
| 25101 | | > hide #34.13 models |
| 25102 | | |
| 25103 | | > show #!35 models |
| 25104 | | |
| 25105 | | > show #39.1 models |
| 25106 | | |
| 25107 | | > fitmap #35.9 inMap #22 |
| 25108 | | |
| 25109 | | Fit molecule COPI_soluble_job155_dhelix.pdb L (#35.9) to map |
| 25110 | | W20_J3988_consensus_resampled.mrc (#22) using 878 atoms |
| 25111 | | average map value = 0.3662, steps = 64 |
| 25112 | | shifted from previous position = 1.16 |
| 25113 | | rotated from previous position = 1.8 degrees |
| 25114 | | atoms outside contour = 302, contour level = 0.28583 |
| 25115 | | |
| 25116 | | Position of COPI_soluble_job155_dhelix.pdb L (#35.9) relative to |
| 25117 | | W20_J3988_consensus_resampled.mrc (#22) coordinates: |
| 25118 | | Matrix rotation and translation |
| 25119 | | -0.95887497 0.16676765 -0.22966788 440.72789628 |
| 25120 | | 0.05962226 -0.67277255 -0.73744307 605.37107525 |
| 25121 | | -0.27749589 -0.72080903 0.63516169 247.24016866 |
| 25122 | | Axis 0.14036124 0.40358181 -0.90411307 |
| 25123 | | Axis point 248.93620927 350.83028914 0.00000000 |
| 25124 | | Rotation angle (degrees) 176.60298227 |
| 25125 | | Shift along axis 82.64480207 |
| 25126 | | |
| 25127 | | |
| 25128 | | > fitmap #35.11 inMap #22 |
| 25129 | | |
| 25130 | | Fit molecule COPI_soluble_job155_dhelix.pdb N (#35.11) to map |
| 25131 | | W20_J3988_consensus_resampled.mrc (#22) using 1064 atoms |
| 25132 | | average map value = 0.384, steps = 72 |
| 25133 | | shifted from previous position = 0.949 |
| 25134 | | rotated from previous position = 1.29 degrees |
| 25135 | | atoms outside contour = 322, contour level = 0.28583 |
| 25136 | | |
| 25137 | | Position of COPI_soluble_job155_dhelix.pdb N (#35.11) relative to |
| 25138 | | W20_J3988_consensus_resampled.mrc (#22) coordinates: |
| 25139 | | Matrix rotation and translation |
| 25140 | | -0.93994555 0.23565898 -0.24691539 415.54469230 |
| 25141 | | 0.01481361 -0.69455698 -0.71928517 620.65417336 |
| 25142 | | -0.34100282 -0.67974661 0.64935478 246.72523257 |
| 25143 | | Axis 0.16251841 0.38673478 -0.90775767 |
| 25144 | | Axis point 249.64126104 349.36740731 0.00000000 |
| 25145 | | Rotation angle (degrees) 173.01304550 |
| 25146 | | Shift along axis 83.59549488 |
| 25147 | | |
| 25148 | | |
| 25149 | | > fitmap #39.1 inMap #22 |
| 25150 | | |
| 25151 | | Fit molecule fold_2025_01_17_alpha1_330gamma1_330zeta_model_0.cif A (#39.1) to |
| 25152 | | map W20_J3988_consensus_resampled.mrc (#22) using 2567 atoms |
| 25153 | | average map value = 0.2763, steps = 92 |
| 25154 | | shifted from previous position = 10.5 |
| 25155 | | rotated from previous position = 9.1 degrees |
| 25156 | | atoms outside contour = 1395, contour level = 0.28583 |
| 25157 | | |
| 25158 | | Position of fold_2025_01_17_alpha1_330gamma1_330zeta_model_0.cif A (#39.1) |
| 25159 | | relative to W20_J3988_consensus_resampled.mrc (#22) coordinates: |
| 25160 | | Matrix rotation and translation |
| 25161 | | 0.78195191 0.59824553 -0.17508140 136.85372786 |
| 25162 | | 0.46784009 -0.74887041 -0.46938125 138.09735041 |
| 25163 | | -0.41191852 0.28512347 -0.86546389 93.90913619 |
| 25164 | | Axis 0.94138597 0.29549867 -0.16270522 |
| 25165 | | Axis point 0.00000000 34.34868013 69.05412162 |
| 25166 | | Rotation angle (degrees) 156.37546309 |
| 25167 | | Shift along axis 154.36025614 |
| 25168 | | |
| 25169 | | |
| 25170 | | > fitmap #42 inMap #22 |
| 25171 | | |
| 25172 | | Fit molecule copy of COPI_cyto_J4025_model M (#42) to map |
| 25173 | | W20_J3988_consensus_resampled.mrc (#22) using 2618 atoms |
| 25174 | | average map value = 0.3079, steps = 52 |
| 25175 | | shifted from previous position = 1.27 |
| 25176 | | rotated from previous position = 2.32 degrees |
| 25177 | | atoms outside contour = 1148, contour level = 0.28583 |
| 25178 | | |
| 25179 | | Position of copy of COPI_cyto_J4025_model M (#42) relative to |
| 25180 | | W20_J3988_consensus_resampled.mrc (#22) coordinates: |
| 25181 | | Matrix rotation and translation |
| 25182 | | -0.16260849 -0.97678601 0.13945458 220.44563836 |
| 25183 | | -0.95843463 0.18994779 0.21289181 227.66250748 |
| 25184 | | -0.23443883 -0.09904008 -0.96707265 255.16502900 |
| 25185 | | Axis -0.64015894 0.76731881 0.03766144 |
| 25186 | | Axis point 225.62353549 0.00000000 102.00127381 |
| 25187 | | Rotation angle (degrees) 165.89874346 |
| 25188 | | Shift along axis 43.17936097 |
| 25189 | | |
| 25190 | | |
| 25191 | | > hide #39.1 models |
| 25192 | | |
| 25193 | | > show #!42 models |
| 25194 | | |
| 25195 | | > combine #34.1 #34.2 #34.3 #34.4 #34.5 #34.6 #34.7 #34.8 #34.9 #34.10 #34.11 |
| 25196 | | > #34.12 #42 #35.9 #35.11 |
| 25197 | | |
| 25198 | | Remapping chain ID 'L' in COPI_soluble_job155_dhelix.pdb L #35.9 to 'N' |
| 25199 | | Remapping chain ID 'N' in COPI_soluble_job155_dhelix.pdb N #35.11 to 'O' |
| 25200 | | |
| 25201 | | > rename #44 COPI_cyto_J3988_model |
| 25202 | | |
| 25203 | | > hide #!42 models |
| 25204 | | |
| 25205 | | > hide #!22 models |
| 25206 | | |
| 25207 | | > show #!24 models |
| 25208 | | |
| 25209 | | > hide #!44 models |
| 25210 | | |
| 25211 | | > hide #!35 models |
| 25212 | | |
| 25213 | | > hide #!24 models |
| 25214 | | |
| 25215 | | > hide #!34 models |
| 25216 | | |
| 25217 | | > show #!24 models |
| 25218 | | |
| 25219 | | > show #!1 models |
| 25220 | | |
| 25221 | | > hide #!1 models |
| 25222 | | |
| 25223 | | > show #!3 models |
| 25224 | | |
| 25225 | | > hide #!3 models |
| 25226 | | |
| 25227 | | > show #!4 models |
| 25228 | | |
| 25229 | | > hide #!24 models |
| 25230 | | |
| 25231 | | > show #!25 models |
| 25232 | | |
| 25233 | | > hide #!4 models |
| 25234 | | |
| 25235 | | > show #!5 models |
| 25236 | | |
| 25237 | | > hide #!5 models |
| 25238 | | |
| 25239 | | > show #!6 models |
| 25240 | | |
| 25241 | | > hide #!25 models |
| 25242 | | |
| 25243 | | > show #!26 models |
| 25244 | | |
| 25245 | | > hide #!26 models |
| 25246 | | |
| 25247 | | > show #!27 models |
| 25248 | | |
| 25249 | | > hide #!6 models |
| 25250 | | |
| 25251 | | > show #!8 models |
| 25252 | | |
| 25253 | | > hide #!27 models |
| 25254 | | |
| 25255 | | > close #35 |
| 25256 | | |
| 25257 | | > close #36 |
| 25258 | | |
| 25259 | | > close #37-39 |
| 25260 | | |
| 25261 | | > close #42 |
| 25262 | | |
| 25263 | | > combine #24.1 #24.2 #24.3 #24.4 #24.5 #24.6 #24.7 #24.8 #24.9 #24.10 #24.11 |
| 25264 | | > #24.12 #24.13 |
| 25265 | | |
| 25266 | | > hide #!8 models |
| 25267 | | |
| 25268 | | > show #!1 models |
| 25269 | | |
| 25270 | | > rename #35 COPI_cyto_J3623_model |
| 25271 | | |
| 25272 | | > hide #!35 models |
| 25273 | | |
| 25274 | | > show #!35 models |
| 25275 | | |
| 25276 | | > combine #25.1 #25.2 #25.3 #25.4 #25.5 #25.6 #25.7 #25.8 #25.9 #25.10 #25.11 |
| 25277 | | > #25.12 #25.13 |
| 25278 | | |
| 25279 | | > rename #36 COPI_cyto_J3987_model |
| 25280 | | |
| 25281 | | > combine #26.1 #26.2 #26.3 #26.4 #26.5 #26.6 #26.7 #26.8 #26.9 #26.10 #26.11 |
| 25282 | | > #26.12 #26.13 |
| 25283 | | |
| 25284 | | > rename #37 COPI_cyto_J4021_model |
| 25285 | | |
| 25286 | | > combine #27.1 #27.2 #27.3 #27.4 #27.5 #27.6 #27.7 #27.8 #27.9 #27.10 #27.11 |
| 25287 | | > #27.12 #27.13 |
| 25288 | | |
| 25289 | | > rename #38 COPI_cyto_J4022_model |
| 25290 | | |
| 25291 | | > combine #28.1 #28.2 #28.3 #28.4 #28.5 #28.6 #28.7 #28.8 #28.9 #28.10 #28.11 |
| 25292 | | > #28.12 #28.13 |
| 25293 | | |
| 25294 | | > rename #39 COPI_cyto_J4023_model |
| 25295 | | |
| 25296 | | > combine #29.1 #29.2 #29.3 #29.4 #29.5 #29.6 #29.7 #29.8 #29.9 #29.10 #29.11 |
| 25297 | | > #29.12 #29.13 |
| 25298 | | |
| 25299 | | > rename #42 COPI_cyto_J4024_model |
| 25300 | | |
| 25301 | | > combine #30.1 #30.2 #30.3 #30.4 #30.5 #30.6 #30.7 #30.8 #30.9 #30.10 #30.11 |
| 25302 | | > #30.12 #30.13 |
| 25303 | | |
| 25304 | | > hide #!42 models |
| 25305 | | |
| 25306 | | > hide #!39 models |
| 25307 | | |
| 25308 | | > hide #!38 models |
| 25309 | | |
| 25310 | | > hide #!37 models |
| 25311 | | |
| 25312 | | > hide #!36 models |
| 25313 | | |
| 25314 | | > hide #!35 models |
| 25315 | | |
| 25316 | | > rename #45 COPI_cyto_J4025_model |
| 25317 | | |
| 25318 | | > save COPI_cyto_J3623_model.pdb #35 |
| 25319 | | |
| 25320 | | > save COPI_cyto_J3987_model.pdb #36 |
| 25321 | | |
| 25322 | | > save COPI_cyto_J4021_model.pdb #37 |
| 25323 | | |
| 25324 | | > save COPI_cyto_J4022_model.pdb #38 |
| 25325 | | |
| 25326 | | > save COPI_cyto_J4023_model.pdb #39 |
| 25327 | | |
| 25328 | | > save COPI_cyto_J4026_model.pdb #40 |
| 25329 | | |
| 25330 | | > save COPI_cyto_J4027_model.pdb #41 |
| 25331 | | |
| 25332 | | > save COPI_cyto_J4024_model.pdb #42 |
| 25333 | | |
| 25334 | | > save COPI_cyto_J4028_model.pdb #43 |
| 25335 | | |
| 25336 | | > save COPI_cyto_J3988_model.pdb #44 |
| 25337 | | |
| 25338 | | > save COPI_cyto_J4025_model.pdb #45 |
| 25339 | | |
| 25340 | | > hide #!45 models |
| 25341 | | |
| 25342 | | > show #!5 models |
| 25343 | | |
| 25344 | | > hide #!1 models |
| 25345 | | |
| 25346 | | > hide #!5 models |
| 25347 | | |
| 25348 | | > show #!4 models |
| 25349 | | |
| 25350 | | > hide #!4 models |
| 25351 | | |
| 25352 | | > show #!6 models |
| 25353 | | |
| 25354 | | > hide #!6 models |
| 25355 | | |
| 25356 | | > show #!8 models |
| 25357 | | |
| 25358 | | > hide #!8 models |
| 25359 | | |
| 25360 | | > show #!10 models |
| 25361 | | |
| 25362 | | > hide #!10 models |
| 25363 | | |
| 25364 | | > show #!10 models |
| 25365 | | |
| 25366 | | > hide #!10 models |
| 25367 | | |
| 25368 | | > show #!6 models |
| 25369 | | |
| 25370 | | > show #!31 models |
| 25371 | | |
| 25372 | | > open |
| 25373 | | > /Users/becca/Desktop/Postdoc/Phenix/W20_MM_cyto_COPI/CombineFocusedMaps_33/composite_map.ccp4 |
| 25374 | | |
| 25375 | | Opened composite_map.ccp4 as #46, grid size 240,240,240, pixel 1.19, shown at |
| 25376 | | level 5.84, step 1, values float32 |
| 25377 | | |
| 25378 | | > rename #46 composite_map_J4021_J4026.ccp4 |
| 25379 | | |
| 25380 | | > hide #!46 models |
| 25381 | | |
| 25382 | | > show #!46 models |
| 25383 | | |
| 25384 | | > hide #!6 models |
| 25385 | | |
| 25386 | | > volume #46 level 4.356 |
| 25387 | | |
| 25388 | | > color #46 darkgrey models |
| 25389 | | |
| 25390 | | > color #46 #a9a9a9c3 models |
| 25391 | | |
| 25392 | | > color #46 #a9a9a9c0 models |
| 25393 | | |
| 25394 | | > color #46 #a9a9a9bf models |
| 25395 | | |
| 25396 | | > hide #!31 models |
| 25397 | | |
| 25398 | | > show #!40 models |
| 25399 | | |
| 25400 | | > save "/Users/becca/Desktop/Postdoc/COPI notebooks and |
| 25401 | | > resources/Processing/W20_MM_cyto_COPI/20250707_W20_COPI_cyto_alignments.cxs" |
| 25402 | | |
| 25403 | | > hide #!46 models |
| 25404 | | |
| 25405 | | > show #!46 models |
| 25406 | | |
| 25407 | | > hide #!46 models |
| 25408 | | |
| 25409 | | > open |
| 25410 | | > /Users/becca/Desktop/Postdoc/Phenix/W20_MM_cyto_COPI/J3987_ResolveCryoEM_34/J3987_denmod_map.ccp4 |
| 25411 | | |
| 25412 | | Opened J3987_denmod_map.ccp4 as #47, grid size 100,124,124, pixel 1.19, shown |
| 25413 | | at level 0.316, step 1, values float32 |
| 25414 | | |
| 25415 | | > volume #47 level 0.168 |
| 25416 | | |
| 25417 | | > volume #47 level 0.08306 |
| 25418 | | |
| 25419 | | > close #47 |
| 25420 | | |
| 25421 | | > open |
| 25422 | | > /Users/becca/Desktop/Postdoc/Phenix/W20_MM_cyto_COPI/J4021_ResolveCryoEM_35/J4021_denmod_map.ccp4 |
| 25423 | | |
| 25424 | | Opened J4021_denmod_map.ccp4 as #47, grid size 92,128,121, pixel 1.19, shown |
| 25425 | | at level 0.263, step 1, values float32 |
| 25426 | | |
| 25427 | | > volume #47 level 0.1546 |
| 25428 | | |
| 25429 | | > volume #47 level 0.0986 |
| 25430 | | |
| 25431 | | > close #47 |
| 25432 | | |
| 25433 | | > open |
| 25434 | | > /Users/becca/Desktop/Postdoc/Phenix/W20_MM_cyto_COPI/J4022_ResolveCryoEM_36/J4022_denmod_map.ccp4 |
| 25435 | | |
| 25436 | | Opened J4022_denmod_map.ccp4 as #47, grid size 92,126,116, pixel 1.19, shown |
| 25437 | | at level 0.256, step 1, values float32 |
| 25438 | | |
| 25439 | | > volume #47 level 0.1136 |
| 25440 | | |
| 25441 | | > close #47 |
| 25442 | | |
| 25443 | | > open |
| 25444 | | > /Users/becca/Desktop/Postdoc/Phenix/W20_MM_cyto_COPI/J4023_ResolveCryoEM_37/J4023_denmod_map.ccp4 |
| 25445 | | |
| 25446 | | Opened J4023_denmod_map.ccp4 as #47, grid size 92,125,117, pixel 1.19, shown |
| 25447 | | at level 0.26, step 1, values float32 |
| 25448 | | |
| 25449 | | > volume #47 level 0.06747 |
| 25450 | | |
| 25451 | | > open |
| 25452 | | > /Users/becca/Desktop/Postdoc/Phenix/W20_MM_cyto_COPI/J4024_ResolveCryoEM_38/J4024_denmod_map.ccp4 |
| 25453 | | |
| 25454 | | Opened J4024_denmod_map.ccp4 as #48, grid size 92,126,119, pixel 1.19, shown |
| 25455 | | at level 0.262, step 1, values float32 |
| 25456 | | |
| 25457 | | > close #47 |
| 25458 | | |
| 25459 | | > volume #48 level 0.1111 |
| 25460 | | |
| 25461 | | > close #48 |
| 25462 | | |
| 25463 | | > open |
| 25464 | | > /Users/becca/Desktop/Postdoc/Phenix/W20_MM_cyto_COPI/J4025_ResolveCryoEM_40/J4025_denmod_map.ccp4 |
| 25465 | | |
| 25466 | | Opened J4025_denmod_map.ccp4 as #47, grid size 91,128,120, pixel 1.19, shown |
| 25467 | | at level 0.235, step 1, values float32 |
| 25468 | | |
| 25469 | | > volume #47 level 0.1354 |
| 25470 | | |
| 25471 | | > volume #47 level 0.09666 |
| 25472 | | |
| 25473 | | > close #47 |
| 25474 | | |
| 25475 | | > save "/Users/becca/Desktop/Postdoc/COPI notebooks and |
| 25476 | | > resources/Processing/W20_MM_cyto_COPI/20250707_W20_COPI_cyto_alignments.cxs" |
| 25477 | | |
| 25478 | | ——— End of log from Mon Jul 7 09:47:13 2025 ——— |
| 25479 | | |
| 25480 | | > view name session-start |
| 25481 | | |
| 25482 | | opened ChimeraX session |
| 25483 | | |
| 25484 | | > show #!46 models |
| 25485 | | |
| 25486 | | > open |
| 25487 | | > /Users/becca/Desktop/Postdoc/Phenix/W20_MM_cyto_COPI/J4021_J4027_CombineFocusedMaps_41/J4021_J4027_composite_map.ccp4 |
| 25488 | | |
| 25489 | | Opened J4021_J4027_composite_map.ccp4 as #47, grid size 240,240,240, pixel |
| 25490 | | 1.19, shown at level 5.81, step 1, values float32 |
| 25491 | | |
| 25492 | | > color #47 darkgrey models |
| 25493 | | |
| 25494 | | > color #47 #a9a9a9c1 models |
| 25495 | | |
| 25496 | | > hide #!47 models |
| 25497 | | |
| 25498 | | > hide #!46 models |
| 25499 | | |
| 25500 | | > hide #!40 models |
| 25501 | | |
| 25502 | | > show #!40 models |
| 25503 | | |
| 25504 | | > hide #!40 models |
| 25505 | | |
| 25506 | | > combine #40 |
| 25507 | | |
| 25508 | | > split #48 chains |
| 25509 | | |
| 25510 | | Split copy of COPI_cyto_J4026_model (#48) into 15 models |
| 25511 | | Chain information for copy of COPI_cyto_J4026_model A #48.1 |
| 25512 | | --- |
| 25513 | | Chain | Description |
| 25514 | | A | No description available |
| 25515 | | |
| 25516 | | Chain information for copy of COPI_cyto_J4026_model B #48.2 |
| 25517 | | --- |
| 25518 | | Chain | Description |
| 25519 | | B | No description available |
| 25520 | | |
| 25521 | | Chain information for copy of COPI_cyto_J4026_model C #48.3 |
| 25522 | | --- |
| 25523 | | Chain | Description |
| 25524 | | C | No description available |
| 25525 | | |
| 25526 | | Chain information for copy of COPI_cyto_J4026_model D #48.4 |
| 25527 | | --- |
| 25528 | | Chain | Description |
| 25529 | | D | No description available |
| 25530 | | |
| 25531 | | Chain information for copy of COPI_cyto_J4026_model E #48.5 |
| 25532 | | --- |
| 25533 | | Chain | Description |
| 25534 | | E | No description available |
| 25535 | | |
| 25536 | | Chain information for copy of COPI_cyto_J4026_model F #48.6 |
| 25537 | | --- |
| 25538 | | Chain | Description |
| 25539 | | F | No description available |
| 25540 | | |
| 25541 | | Chain information for copy of COPI_cyto_J4026_model G #48.7 |
| 25542 | | --- |
| 25543 | | Chain | Description |
| 25544 | | G | No description available |
| 25545 | | |
| 25546 | | Chain information for copy of COPI_cyto_J4026_model H #48.8 |
| 25547 | | --- |
| 25548 | | Chain | Description |
| 25549 | | H | No description available |
| 25550 | | |
| 25551 | | Chain information for copy of COPI_cyto_J4026_model I #48.9 |
| 25552 | | --- |
| 25553 | | Chain | Description |
| 25554 | | I | No description available |
| 25555 | | |
| 25556 | | Chain information for copy of COPI_cyto_J4026_model J #48.10 |
| 25557 | | --- |
| 25558 | | Chain | Description |
| 25559 | | J | No description available |
| 25560 | | |
| 25561 | | Chain information for copy of COPI_cyto_J4026_model K #48.11 |
| 25562 | | --- |
| 25563 | | Chain | Description |
| 25564 | | K | No description available |
| 25565 | | |
| 25566 | | Chain information for copy of COPI_cyto_J4026_model L #48.12 |
| 25567 | | --- |
| 25568 | | Chain | Description |
| 25569 | | L | No description available |
| 25570 | | |
| 25571 | | Chain information for copy of COPI_cyto_J4026_model M #48.13 |
| 25572 | | --- |
| 25573 | | Chain | Description |
| 25574 | | M | No description available |
| 25575 | | |
| 25576 | | Chain information for copy of COPI_cyto_J4026_model N #48.14 |
| 25577 | | --- |
| 25578 | | Chain | Description |
| 25579 | | N | No description available |
| 25580 | | |
| 25581 | | Chain information for copy of COPI_cyto_J4026_model O #48.15 |
| 25582 | | --- |
| 25583 | | Chain | Description |
| 25584 | | O | No description available |
| 25585 | | |
| 25586 | | |
| 25587 | | > show #!46 models |
| 25588 | | |
| 25589 | | > fitmap #48.1 inMap #46 |
| 25590 | | |
| 25591 | | Fit molecule copy of COPI_cyto_J4026_model A (#48.1) to map |
| 25592 | | composite_map_J4021_J4026.ccp4 (#46) using 2590 atoms |
| 25593 | | average map value = 8.012, steps = 44 |
| 25594 | | shifted from previous position = 0.024 |
| 25595 | | rotated from previous position = 0.0413 degrees |
| 25596 | | atoms outside contour = 449, contour level = 4.3561 |
| 25597 | | |
| 25598 | | Position of copy of COPI_cyto_J4026_model A (#48.1) relative to |
| 25599 | | composite_map_J4021_J4026.ccp4 (#46) coordinates: |
| 25600 | | Matrix rotation and translation |
| 25601 | | -0.42161671 -0.88866766 -0.18030295 281.35767523 |
| 25602 | | -0.78137178 0.25516130 0.56951811 159.89900138 |
| 25603 | | -0.46010598 0.38100198 -0.80196009 237.46670115 |
| 25604 | | Axis -0.53247943 0.79032688 0.30306613 |
| 25605 | | Axis point 207.11724288 0.00000000 70.73686916 |
| 25606 | | Rotation angle (degrees) 169.80392724 |
| 25607 | | Shift along axis 48.52341756 |
| 25608 | | |
| 25609 | | |
| 25610 | | > fitmap #48.2 inMap #46 |
| 25611 | | |
| 25612 | | Fit molecule copy of COPI_cyto_J4026_model B (#48.2) to map |
| 25613 | | composite_map_J4021_J4026.ccp4 (#46) using 2426 atoms |
| 25614 | | average map value = 7.643, steps = 48 |
| 25615 | | shifted from previous position = 0.0352 |
| 25616 | | rotated from previous position = 0.149 degrees |
| 25617 | | atoms outside contour = 405, contour level = 4.3561 |
| 25618 | | |
| 25619 | | Position of copy of COPI_cyto_J4026_model B (#48.2) relative to |
| 25620 | | composite_map_J4021_J4026.ccp4 (#46) coordinates: |
| 25621 | | Matrix rotation and translation |
| 25622 | | -0.42061793 -0.88953902 -0.17832811 281.05427064 |
| 25623 | | -0.78226434 0.25604617 0.56789336 160.17141808 |
| 25624 | | -0.45950307 0.37836585 -0.80355226 237.63647217 |
| 25625 | | Axis -0.53290254 0.79059154 0.30162873 |
| 25626 | | Axis point 207.14962646 0.00000000 70.86650714 |
| 25627 | | Rotation angle (degrees) 169.75686245 |
| 25628 | | Shift along axis 48.53362146 |
| 25629 | | |
| 25630 | | |
| 25631 | | > fitmap #48.3 inMap #46 |
| 25632 | | |
| 25633 | | Fit molecule copy of COPI_cyto_J4026_model C (#48.3) to map |
| 25634 | | composite_map_J4021_J4026.ccp4 (#46) using 1612 atoms |
| 25635 | | average map value = 6.339, steps = 64 |
| 25636 | | shifted from previous position = 0.137 |
| 25637 | | rotated from previous position = 1.31 degrees |
| 25638 | | atoms outside contour = 282, contour level = 4.3561 |
| 25639 | | |
| 25640 | | Position of copy of COPI_cyto_J4026_model C (#48.3) relative to |
| 25641 | | composite_map_J4021_J4026.ccp4 (#46) coordinates: |
| 25642 | | Matrix rotation and translation |
| 25643 | | -0.43767621 -0.88321497 -0.16843654 281.87335410 |
| 25644 | | -0.76783552 0.26967498 0.58112309 157.06406865 |
| 25645 | | -0.46783349 0.38367530 -0.79619414 237.77730124 |
| 25646 | | Axis -0.52408907 0.79469449 0.30625367 |
| 25647 | | Axis point 206.03117664 0.00000000 70.20592740 |
| 25648 | | Rotation angle (degrees) 169.14218802 |
| 25649 | | Shift along axis 49.91138003 |
| 25650 | | |
| 25651 | | |
| 25652 | | > fitmap #48.4 inMap #46 |
| 25653 | | |
| 25654 | | Fit molecule copy of COPI_cyto_J4026_model D (#48.4) to map |
| 25655 | | composite_map_J4021_J4026.ccp4 (#46) using 4745 atoms |
| 25656 | | average map value = 6.217, steps = 64 |
| 25657 | | shifted from previous position = 0.346 |
| 25658 | | rotated from previous position = 0.851 degrees |
| 25659 | | atoms outside contour = 894, contour level = 4.3561 |
| 25660 | | |
| 25661 | | Position of copy of COPI_cyto_J4026_model D (#48.4) relative to |
| 25662 | | composite_map_J4021_J4026.ccp4 (#46) coordinates: |
| 25663 | | Matrix rotation and translation |
| 25664 | | -0.42924156 -0.88289723 -0.19037904 282.64245635 |
| 25665 | | -0.77884347 0.25509758 0.57299920 159.11506089 |
| 25666 | | -0.45733417 0.39423054 -0.79713722 235.42666761 |
| 25667 | | Axis -0.52934994 0.79047793 0.30811245 |
| 25668 | | Axis point 206.45398809 0.00000000 70.11358015 |
| 25669 | | Rotation angle (degrees) 170.27864285 |
| 25670 | | Shift along axis 48.69806493 |
| 25671 | | |
| 25672 | | |
| 25673 | | > fitmap #48.5 inMap #46 |
| 25674 | | |
| 25675 | | Fit molecule copy of COPI_cyto_J4026_model E (#48.5) to map |
| 25676 | | composite_map_J4021_J4026.ccp4 (#46) using 1876 atoms |
| 25677 | | average map value = 6.728, steps = 28 |
| 25678 | | shifted from previous position = 0.0537 |
| 25679 | | rotated from previous position = 0.205 degrees |
| 25680 | | atoms outside contour = 414, contour level = 4.3561 |
| 25681 | | |
| 25682 | | Position of copy of COPI_cyto_J4026_model E (#48.5) relative to |
| 25683 | | composite_map_J4021_J4026.ccp4 (#46) coordinates: |
| 25684 | | Matrix rotation and translation |
| 25685 | | -0.41906414 -0.89016033 -0.17888502 280.98895035 |
| 25686 | | -0.78296435 0.25453348 0.56760863 160.29649800 |
| 25687 | | -0.45973045 0.37792501 -0.80362965 237.75201321 |
| 25688 | | Axis -0.53364295 0.79011139 0.30157785 |
| 25689 | | Axis point 207.27636606 0.00000000 70.89197237 |
| 25690 | | Rotation angle (degrees) 169.76271203 |
| 25691 | | Shift along axis 48.40505872 |
| 25692 | | |
| 25693 | | |
| 25694 | | > fitmap #48.6 inMap #46 |
| 25695 | | |
| 25696 | | Fit molecule copy of COPI_cyto_J4026_model F (#48.6) to map |
| 25697 | | composite_map_J4021_J4026.ccp4 (#46) using 2400 atoms |
| 25698 | | average map value = 4.933, steps = 60 |
| 25699 | | shifted from previous position = 4.71 |
| 25700 | | rotated from previous position = 7.6 degrees |
| 25701 | | atoms outside contour = 678, contour level = 4.3561 |
| 25702 | | |
| 25703 | | Position of copy of COPI_cyto_J4026_model F (#48.6) relative to |
| 25704 | | composite_map_J4021_J4026.ccp4 (#46) coordinates: |
| 25705 | | Matrix rotation and translation |
| 25706 | | -0.53669929 -0.82504557 -0.17678715 281.80609510 |
| 25707 | | -0.72189432 0.34051286 0.60242808 144.12665157 |
| 25708 | | -0.43683232 0.45094436 -0.77834872 227.64701078 |
| 25709 | | Axis -0.47615916 0.81740061 0.32423556 |
| 25710 | | Axis point 193.11631392 -0.00000000 70.10143524 |
| 25711 | | Rotation angle (degrees) 170.84717448 |
| 25712 | | Shift along axis 57.43591697 |
| 25713 | | |
| 25714 | | |
| 25715 | | > fitmap #48.7 inMap #46 |
| 25716 | | |
| 25717 | | Fit molecule copy of COPI_cyto_J4026_model G (#48.7) to map |
| 25718 | | composite_map_J4021_J4026.ccp4 (#46) using 2302 atoms |
| 25719 | | average map value = 5.406, steps = 80 |
| 25720 | | shifted from previous position = 4.73 |
| 25721 | | rotated from previous position = 4.21 degrees |
| 25722 | | atoms outside contour = 580, contour level = 4.3561 |
| 25723 | | |
| 25724 | | Position of copy of COPI_cyto_J4026_model G (#48.7) relative to |
| 25725 | | composite_map_J4021_J4026.ccp4 (#46) coordinates: |
| 25726 | | Matrix rotation and translation |
| 25727 | | -0.48330635 -0.85702087 -0.17869027 283.39251275 |
| 25728 | | -0.74444396 0.29492363 0.59901858 152.16224396 |
| 25729 | | -0.46067144 0.42253437 -0.78054247 232.42661347 |
| 25730 | | Axis -0.50253361 0.80293313 0.32055945 |
| 25731 | | Axis point 201.16168457 -0.00000000 68.72114991 |
| 25732 | | Rotation angle (degrees) 169.88674773 |
| 25733 | | Shift along axis 54.26839065 |
| 25734 | | |
| 25735 | | |
| 25736 | | > fitmap #48.8 inMap #46 |
| 25737 | | |
| 25738 | | Fit molecule copy of COPI_cyto_J4026_model H (#48.8) to map |
| 25739 | | composite_map_J4021_J4026.ccp4 (#46) using 420 atoms |
| 25740 | | average map value = 7.082, steps = 56 |
| 25741 | | shifted from previous position = 3.46 |
| 25742 | | rotated from previous position = 2.58 degrees |
| 25743 | | atoms outside contour = 93, contour level = 4.3561 |
| 25744 | | |
| 25745 | | Position of copy of COPI_cyto_J4026_model H (#48.8) relative to |
| 25746 | | composite_map_J4021_J4026.ccp4 (#46) coordinates: |
| 25747 | | Matrix rotation and translation |
| 25748 | | -0.46181347 -0.87023765 -0.17150736 282.95432862 |
| 25749 | | -0.76161078 0.28994955 0.57955009 156.07549407 |
| 25750 | | -0.45461782 0.39826589 -0.79668497 236.09755101 |
| 25751 | | Axis -0.51312841 0.80134959 0.30747044 |
| 25752 | | Axis point 203.66513828 0.00000000 70.89396293 |
| 25753 | | Rotation angle (degrees) 169.82553836 |
| 25754 | | Shift along axis 52.47214622 |
| 25755 | | |
| 25756 | | |
| 25757 | | > fitmap #48.9 inMap #46 |
| 25758 | | |
| 25759 | | Fit molecule copy of COPI_cyto_J4026_model I (#48.9) to map |
| 25760 | | composite_map_J4021_J4026.ccp4 (#46) using 1573 atoms |
| 25761 | | average map value = 5.892, steps = 72 |
| 25762 | | shifted from previous position = 1.08 |
| 25763 | | rotated from previous position = 2.82 degrees |
| 25764 | | atoms outside contour = 307, contour level = 4.3561 |
| 25765 | | |
| 25766 | | Position of copy of COPI_cyto_J4026_model I (#48.9) relative to |
| 25767 | | composite_map_J4021_J4026.ccp4 (#46) coordinates: |
| 25768 | | Matrix rotation and translation |
| 25769 | | -0.44042374 -0.87092293 -0.21799127 283.13278743 |
| 25770 | | -0.77489855 0.24614258 0.58219077 160.68330197 |
| 25771 | | -0.45338635 0.42533175 -0.78328394 232.61931758 |
| 25772 | | Axis -0.52509607 0.78800081 0.32144805 |
| 25773 | | Axis point 205.38082265 0.00000000 68.72443099 |
| 25774 | | Rotation angle (degrees) 171.41003245 |
| 25775 | | Shift along axis 52.72168395 |
| 25776 | | |
| 25777 | | |
| 25778 | | > fitmap #48.10 inMap #46 |
| 25779 | | |
| 25780 | | Fit molecule copy of COPI_cyto_J4026_model J (#48.10) to map |
| 25781 | | composite_map_J4021_J4026.ccp4 (#46) using 1112 atoms |
| 25782 | | average map value = 6.367, steps = 68 |
| 25783 | | shifted from previous position = 0.853 |
| 25784 | | rotated from previous position = 2.18 degrees |
| 25785 | | atoms outside contour = 324, contour level = 4.3561 |
| 25786 | | |
| 25787 | | Position of copy of COPI_cyto_J4026_model J (#48.10) relative to |
| 25788 | | composite_map_J4021_J4026.ccp4 (#46) coordinates: |
| 25789 | | Matrix rotation and translation |
| 25790 | | -0.39606742 -0.89516479 -0.20447643 281.46766551 |
| 25791 | | -0.80125621 0.22817550 0.55310438 165.26116045 |
| 25792 | | -0.44846305 0.38290463 -0.80762922 236.59702773 |
| 25793 | | Axis -0.54558979 0.78211990 0.30103197 |
| 25794 | | Axis point 209.53544999 0.00000000 71.09106362 |
| 25795 | | Rotation angle (degrees) 171.02649245 |
| 25796 | | Shift along axis 46.91142523 |
| 25797 | | |
| 25798 | | |
| 25799 | | > fitmap #48.12 inMap #46 |
| 25800 | | |
| 25801 | | Fit molecule copy of COPI_cyto_J4026_model L (#48.12) to map |
| 25802 | | composite_map_J4021_J4026.ccp4 (#46) using 1420 atoms |
| 25803 | | average map value = 6, steps = 48 |
| 25804 | | shifted from previous position = 0.00534 |
| 25805 | | rotated from previous position = 0.0308 degrees |
| 25806 | | atoms outside contour = 490, contour level = 4.3561 |
| 25807 | | |
| 25808 | | Position of copy of COPI_cyto_J4026_model L (#48.12) relative to |
| 25809 | | composite_map_J4021_J4026.ccp4 (#46) coordinates: |
| 25810 | | Matrix rotation and translation |
| 25811 | | -0.42201385 -0.88851995 -0.18010170 281.36850791 |
| 25812 | | -0.78123838 0.25562354 0.56949383 159.86937778 |
| 25813 | | -0.45996839 0.38103664 -0.80202255 237.46846236 |
| 25814 | | Axis -0.53229101 0.79047409 0.30301320 |
| 25815 | | Axis point 207.08142201 0.00000000 70.75583573 |
| 25816 | | Rotation angle (degrees) 169.80350204 |
| 25817 | | Shift along axis 48.55875296 |
| 25818 | | |
| 25819 | | |
| 25820 | | > fitmap #48.13 inMap #46 |
| 25821 | | |
| 25822 | | Fit molecule copy of COPI_cyto_J4026_model M (#48.13) to map |
| 25823 | | composite_map_J4021_J4026.ccp4 (#46) using 2567 atoms |
| 25824 | | average map value = 6.934, steps = 48 |
| 25825 | | shifted from previous position = 0.0327 |
| 25826 | | rotated from previous position = 0.0247 degrees |
| 25827 | | atoms outside contour = 577, contour level = 4.3561 |
| 25828 | | |
| 25829 | | Position of copy of COPI_cyto_J4026_model M (#48.13) relative to |
| 25830 | | composite_map_J4021_J4026.ccp4 (#46) coordinates: |
| 25831 | | Matrix rotation and translation |
| 25832 | | -0.42198871 -0.88852999 -0.18011109 281.34034070 |
| 25833 | | -0.78150296 0.25580865 0.56904751 159.96958762 |
| 25834 | | -0.45954180 0.38088898 -0.80233717 237.44017007 |
| 25835 | | Axis -0.53232061 0.79053934 0.30279089 |
| 25836 | | Axis point 207.07606122 0.00000000 70.77828437 |
| 25837 | | Rotation angle (degrees) 169.82040522 |
| 25838 | | Shift along axis 48.59371022 |
| 25839 | | |
| 25840 | | |
| 25841 | | > fitmap #48.14 inMap #46 |
| 25842 | | |
| 25843 | | Fit molecule copy of COPI_cyto_J4026_model N (#48.14) to map |
| 25844 | | composite_map_J4021_J4026.ccp4 (#46) using 930 atoms |
| 25845 | | average map value = 7.254, steps = 40 |
| 25846 | | shifted from previous position = 0.0472 |
| 25847 | | rotated from previous position = 0.247 degrees |
| 25848 | | atoms outside contour = 214, contour level = 4.3561 |
| 25849 | | |
| 25850 | | Position of copy of COPI_cyto_J4026_model N (#48.14) relative to |
| 25851 | | composite_map_J4021_J4026.ccp4 (#46) coordinates: |
| 25852 | | Matrix rotation and translation |
| 25853 | | -0.42294225 -0.88896586 -0.17566891 281.09587079 |
| 25854 | | -0.78004341 0.25851684 0.56982571 159.40297655 |
| 25855 | | -0.46114222 0.37803274 -0.80276965 237.94268675 |
| 25856 | | Axis -0.53164432 0.79132341 0.30192975 |
| 25857 | | Axis point 206.92420625 0.00000000 70.92756128 |
| 25858 | | Rotation angle (degrees) 169.60827201 |
| 25859 | | Shift along axis 48.53825925 |
| 25860 | | |
| 25861 | | |
| 25862 | | > fitmap #48.15 inMap #46 |
| 25863 | | |
| 25864 | | Fit molecule copy of COPI_cyto_J4026_model O (#48.15) to map |
| 25865 | | composite_map_J4021_J4026.ccp4 (#46) using 1064 atoms |
| 25866 | | average map value = 6.216, steps = 60 |
| 25867 | | shifted from previous position = 0.0698 |
| 25868 | | rotated from previous position = 0.468 degrees |
| 25869 | | atoms outside contour = 130, contour level = 4.3561 |
| 25870 | | |
| 25871 | | Position of copy of COPI_cyto_J4026_model O (#48.15) relative to |
| 25872 | | composite_map_J4021_J4026.ccp4 (#46) coordinates: |
| 25873 | | Matrix rotation and translation |
| 25874 | | -0.41919869 -0.88836490 -0.18729731 281.44552791 |
| 25875 | | -0.78315395 0.24946223 0.56959504 160.73613068 |
| 25876 | | -0.45928463 0.38545612 -0.80030071 236.81019199 |
| 25877 | | Axis -0.53389619 0.78860565 0.30505080 |
| 25878 | | Axis point 207.35794189 0.00000000 70.38133823 |
| 25879 | | Rotation angle (degrees) 170.06980434 |
| 25880 | | Shift along axis 48.73386479 |
| 25881 | | |
| 25882 | | |
| 25883 | | > color #48.13 gammav3 |
| 25884 | | |
| 25885 | | > color #48.14 gammav3 |
| 25886 | | |
| 25887 | | > color #48.15 gammav3 |
| 25888 | | |
| 25889 | | > combine #48.1 #48.2 #48.3 #48.4 #48.5 #48.6 #48.7 #48.8 #48.9 #48.10 #48.11 |
| 25890 | | > #48.12 #48.13 #48.14 #48.15 |
| 25891 | | |
| 25892 | | > rename #49 Model_for_composite_J4021_J4026 |
| 25893 | | |
| 25894 | | > hide #!49 models |
| 25895 | | |
| 25896 | | > hide #!46 models |
| 25897 | | |
| 25898 | | > show #!47 models |
| 25899 | | |
| 25900 | | > close #48 |
| 25901 | | |
| 25902 | | > combine #41 |
| 25903 | | |
| 25904 | | > split #48 chains |
| 25905 | | |
| 25906 | | Split copy of COPI_cyto_J4027_model (#48) into 15 models |
| 25907 | | Chain information for copy of COPI_cyto_J4027_model A #48.1 |
| 25908 | | --- |
| 25909 | | Chain | Description |
| 25910 | | A | No description available |
| 25911 | | |
| 25912 | | Chain information for copy of COPI_cyto_J4027_model B #48.2 |
| 25913 | | --- |
| 25914 | | Chain | Description |
| 25915 | | B | No description available |
| 25916 | | |
| 25917 | | Chain information for copy of COPI_cyto_J4027_model C #48.3 |
| 25918 | | --- |
| 25919 | | Chain | Description |
| 25920 | | C | No description available |
| 25921 | | |
| 25922 | | Chain information for copy of COPI_cyto_J4027_model D #48.4 |
| 25923 | | --- |
| 25924 | | Chain | Description |
| 25925 | | D | No description available |
| 25926 | | |
| 25927 | | Chain information for copy of COPI_cyto_J4027_model E #48.5 |
| 25928 | | --- |
| 25929 | | Chain | Description |
| 25930 | | E | No description available |
| 25931 | | |
| 25932 | | Chain information for copy of COPI_cyto_J4027_model F #48.6 |
| 25933 | | --- |
| 25934 | | Chain | Description |
| 25935 | | F | No description available |
| 25936 | | |
| 25937 | | Chain information for copy of COPI_cyto_J4027_model G #48.7 |
| 25938 | | --- |
| 25939 | | Chain | Description |
| 25940 | | G | No description available |
| 25941 | | |
| 25942 | | Chain information for copy of COPI_cyto_J4027_model H #48.8 |
| 25943 | | --- |
| 25944 | | Chain | Description |
| 25945 | | H | No description available |
| 25946 | | |
| 25947 | | Chain information for copy of COPI_cyto_J4027_model I #48.9 |
| 25948 | | --- |
| 25949 | | Chain | Description |
| 25950 | | I | No description available |
| 25951 | | |
| 25952 | | Chain information for copy of COPI_cyto_J4027_model J #48.10 |
| 25953 | | --- |
| 25954 | | Chain | Description |
| 25955 | | J | No description available |
| 25956 | | |
| 25957 | | Chain information for copy of COPI_cyto_J4027_model K #48.11 |
| 25958 | | --- |
| 25959 | | Chain | Description |
| 25960 | | K | No description available |
| 25961 | | |
| 25962 | | Chain information for copy of COPI_cyto_J4027_model L #48.12 |
| 25963 | | --- |
| 25964 | | Chain | Description |
| 25965 | | L | No description available |
| 25966 | | |
| 25967 | | Chain information for copy of COPI_cyto_J4027_model M #48.13 |
| 25968 | | --- |
| 25969 | | Chain | Description |
| 25970 | | M | No description available |
| 25971 | | |
| 25972 | | Chain information for copy of COPI_cyto_J4027_model N #48.14 |
| 25973 | | --- |
| 25974 | | Chain | Description |
| 25975 | | N | No description available |
| 25976 | | |
| 25977 | | Chain information for copy of COPI_cyto_J4027_model O #48.15 |
| 25978 | | --- |
| 25979 | | Chain | Description |
| 25980 | | O | No description available |
| 25981 | | |
| 25982 | | |
| 25983 | | > color #48.13 gammav3 |
| 25984 | | |
| 25985 | | > color #48.14 gammav3 |
| 25986 | | |
| 25987 | | > color #48.15 gammav3 |
| 25988 | | |
| 25989 | | > fitmap #48.15 inMap #46 |
| 25990 | | |
| 25991 | | Fit molecule copy of COPI_cyto_J4027_model O (#48.15) to map |
| 25992 | | composite_map_J4021_J4026.ccp4 (#46) using 1064 atoms |
| 25993 | | average map value = 6.216, steps = 68 |
| 25994 | | shifted from previous position = 0.926 |
| 25995 | | rotated from previous position = 3.78 degrees |
| 25996 | | atoms outside contour = 131, contour level = 4.3561 |
| 25997 | | |
| 25998 | | Position of copy of COPI_cyto_J4027_model O (#48.15) relative to |
| 25999 | | composite_map_J4021_J4026.ccp4 (#46) coordinates: |
| 26000 | | Matrix rotation and translation |
| 26001 | | -0.34404909 -0.93260747 -0.10896575 269.91254838 |
| 26002 | | -0.80984309 0.23600764 0.53707968 166.69850821 |
| 26003 | | -0.47516777 0.27302693 -0.83646393 251.15790315 |
| 26004 | | Axis -0.56437651 0.78270657 0.26239203 |
| 26005 | | Axis point 213.21055464 0.00000000 75.30227951 |
| 26006 | | Rotation angle (degrees) 166.47125621 |
| 26007 | | Shift along axis 44.04554723 |
| 26008 | | |
| 26009 | | |
| 26010 | | > fitmap #48.14 inMap #46 |
| 26011 | | |
| 26012 | | Fit molecule copy of COPI_cyto_J4027_model N (#48.14) to map |
| 26013 | | composite_map_J4021_J4026.ccp4 (#46) using 930 atoms |
| 26014 | | average map value = 7.254, steps = 68 |
| 26015 | | shifted from previous position = 4.09 |
| 26016 | | rotated from previous position = 12.1 degrees |
| 26017 | | atoms outside contour = 214, contour level = 4.3561 |
| 26018 | | |
| 26019 | | Position of copy of COPI_cyto_J4027_model N (#48.14) relative to |
| 26020 | | composite_map_J4021_J4026.ccp4 (#46) coordinates: |
| 26021 | | Matrix rotation and translation |
| 26022 | | -0.29521822 -0.95538003 0.00975828 254.00941556 |
| 26023 | | -0.83564878 0.26314499 0.48212638 171.86630354 |
| 26024 | | -0.46318175 0.13417799 -0.87604735 264.92556241 |
| 26025 | | Axis -0.58065970 0.78924699 0.19980868 |
| 26026 | | Axis point 215.16338827 0.00000000 83.71664964 |
| 26027 | | Rotation angle (degrees) 162.56553634 |
| 26028 | | Shift along axis 41.08635962 |
| 26029 | | |
| 26030 | | |
| 26031 | | > fitmap #48.13 inMap #46 |
| 26032 | | |
| 26033 | | Fit molecule copy of COPI_cyto_J4027_model M (#48.13) to map |
| 26034 | | composite_map_J4021_J4026.ccp4 (#46) using 2567 atoms |
| 26035 | | average map value = 6.934, steps = 184 |
| 26036 | | shifted from previous position = 24.3 |
| 26037 | | rotated from previous position = 38.5 degrees |
| 26038 | | atoms outside contour = 574, contour level = 4.3561 |
| 26039 | | |
| 26040 | | Position of copy of COPI_cyto_J4027_model M (#48.13) relative to |
| 26041 | | composite_map_J4021_J4026.ccp4 (#46) coordinates: |
| 26042 | | Matrix rotation and translation |
| 26043 | | -0.06511231 -0.96101824 0.26870867 188.55069019 |
| 26044 | | -0.97216064 0.12183140 0.20015198 237.48407748 |
| 26045 | | -0.22508686 -0.24819563 -0.94219682 265.26082374 |
| 26046 | | Axis -0.67212131 0.74025262 -0.01670366 |
| 26047 | | Axis point 232.93504959 0.00000000 109.96611159 |
| 26048 | | Rotation angle (degrees) 160.51672010 |
| 26049 | | Shift along axis 44.63844706 |
| 26050 | | |
| 26051 | | |
| 26052 | | > fitmap #48.13 inMap #47 |
| 26053 | | |
| 26054 | | Fit molecule copy of COPI_cyto_J4027_model M (#48.13) to map |
| 26055 | | J4021_J4027_composite_map.ccp4 (#47) using 2567 atoms |
| 26056 | | average map value = 5.305, steps = 140 |
| 26057 | | shifted from previous position = 20.1 |
| 26058 | | rotated from previous position = 23.5 degrees |
| 26059 | | atoms outside contour = 1497, contour level = 5.8074 |
| 26060 | | |
| 26061 | | Position of copy of COPI_cyto_J4027_model M (#48.13) relative to |
| 26062 | | J4021_J4027_composite_map.ccp4 (#47) coordinates: |
| 26063 | | Matrix rotation and translation |
| 26064 | | -0.20584357 -0.97794249 -0.03545317 247.21508377 |
| 26065 | | -0.89336671 0.17300799 0.41468563 196.10217944 |
| 26066 | | -0.39940501 0.11703305 -0.90927384 267.43384909 |
| 26067 | | Axis -0.62307640 0.76186070 0.17704255 |
| 26068 | | Axis point 224.46487691 0.00000000 89.15375612 |
| 26069 | | Rotation angle (degrees) 166.18089851 |
| 26070 | | Shift along axis 42.71583076 |
| 26071 | | |
| 26072 | | |
| 26073 | | > close #48 |
| 26074 | | |
| 26075 | | > combine #41 |
| 26076 | | |
| 26077 | | > split #48 chains |
| 26078 | | |
| 26079 | | Split copy of COPI_cyto_J4027_model (#48) into 15 models |
| 26080 | | Chain information for copy of COPI_cyto_J4027_model A #48.1 |
| 26081 | | --- |
| 26082 | | Chain | Description |
| 26083 | | A | No description available |
| 26084 | | |
| 26085 | | Chain information for copy of COPI_cyto_J4027_model B #48.2 |
| 26086 | | --- |
| 26087 | | Chain | Description |
| 26088 | | B | No description available |
| 26089 | | |
| 26090 | | Chain information for copy of COPI_cyto_J4027_model C #48.3 |
| 26091 | | --- |
| 26092 | | Chain | Description |
| 26093 | | C | No description available |
| 26094 | | |
| 26095 | | Chain information for copy of COPI_cyto_J4027_model D #48.4 |
| 26096 | | --- |
| 26097 | | Chain | Description |
| 26098 | | D | No description available |
| 26099 | | |
| 26100 | | Chain information for copy of COPI_cyto_J4027_model E #48.5 |
| 26101 | | --- |
| 26102 | | Chain | Description |
| 26103 | | E | No description available |
| 26104 | | |
| 26105 | | Chain information for copy of COPI_cyto_J4027_model F #48.6 |
| 26106 | | --- |
| 26107 | | Chain | Description |
| 26108 | | F | No description available |
| 26109 | | |
| 26110 | | Chain information for copy of COPI_cyto_J4027_model G #48.7 |
| 26111 | | --- |
| 26112 | | Chain | Description |
| 26113 | | G | No description available |
| 26114 | | |
| 26115 | | Chain information for copy of COPI_cyto_J4027_model H #48.8 |
| 26116 | | --- |
| 26117 | | Chain | Description |
| 26118 | | H | No description available |
| 26119 | | |
| 26120 | | Chain information for copy of COPI_cyto_J4027_model I #48.9 |
| 26121 | | --- |
| 26122 | | Chain | Description |
| 26123 | | I | No description available |
| 26124 | | |
| 26125 | | Chain information for copy of COPI_cyto_J4027_model J #48.10 |
| 26126 | | --- |
| 26127 | | Chain | Description |
| 26128 | | J | No description available |
| 26129 | | |
| 26130 | | Chain information for copy of COPI_cyto_J4027_model K #48.11 |
| 26131 | | --- |
| 26132 | | Chain | Description |
| 26133 | | K | No description available |
| 26134 | | |
| 26135 | | Chain information for copy of COPI_cyto_J4027_model L #48.12 |
| 26136 | | --- |
| 26137 | | Chain | Description |
| 26138 | | L | No description available |
| 26139 | | |
| 26140 | | Chain information for copy of COPI_cyto_J4027_model M #48.13 |
| 26141 | | --- |
| 26142 | | Chain | Description |
| 26143 | | M | No description available |
| 26144 | | |
| 26145 | | Chain information for copy of COPI_cyto_J4027_model N #48.14 |
| 26146 | | --- |
| 26147 | | Chain | Description |
| 26148 | | N | No description available |
| 26149 | | |
| 26150 | | Chain information for copy of COPI_cyto_J4027_model O #48.15 |
| 26151 | | --- |
| 26152 | | Chain | Description |
| 26153 | | O | No description available |
| 26154 | | |
| 26155 | | |
| 26156 | | > color #48.13 gammav3 |
| 26157 | | |
| 26158 | | > color #48.14 gammav3 |
| 26159 | | |
| 26160 | | > color #48.15 gammav3 |
| 26161 | | |
| 26162 | | > fitmap #48.15 inMap #47 |
| 26163 | | |
| 26164 | | Fit molecule copy of COPI_cyto_J4027_model O (#48.15) to map |
| 26165 | | J4021_J4027_composite_map.ccp4 (#47) using 1064 atoms |
| 26166 | | average map value = 7.175, steps = 80 |
| 26167 | | shifted from previous position = 0.0377 |
| 26168 | | rotated from previous position = 0.0512 degrees |
| 26169 | | atoms outside contour = 362, contour level = 5.8074 |
| 26170 | | |
| 26171 | | Position of copy of COPI_cyto_J4027_model O (#48.15) relative to |
| 26172 | | J4021_J4027_composite_map.ccp4 (#47) coordinates: |
| 26173 | | Matrix rotation and translation |
| 26174 | | -0.36445970 -0.91742956 -0.15966261 274.93147533 |
| 26175 | | -0.79741803 0.21892271 0.56231428 164.22520611 |
| 26176 | | -0.48092997 0.33225874 -0.81136337 243.41623113 |
| 26177 | | Axis -0.55708089 0.77795083 0.29060864 |
| 26178 | | Axis point 211.70950704 0.00000000 70.89847596 |
| 26179 | | Rotation angle (degrees) 168.08366956 |
| 26180 | | Shift along axis 45.33892553 |
| 26181 | | |
| 26182 | | |
| 26183 | | > fitmap #48.14 inMap #47 |
| 26184 | | |
| 26185 | | Fit molecule copy of COPI_cyto_J4027_model N (#48.14) to map |
| 26186 | | J4021_J4027_composite_map.ccp4 (#47) using 930 atoms |
| 26187 | | average map value = 7.843, steps = 48 |
| 26188 | | shifted from previous position = 0.0452 |
| 26189 | | rotated from previous position = 0.236 degrees |
| 26190 | | atoms outside contour = 308, contour level = 5.8074 |
| 26191 | | |
| 26192 | | Position of copy of COPI_cyto_J4027_model N (#48.14) relative to |
| 26193 | | J4021_J4027_composite_map.ccp4 (#47) coordinates: |
| 26194 | | Matrix rotation and translation |
| 26195 | | -0.36749036 -0.91663154 -0.15728153 275.08372435 |
| 26196 | | -0.79611294 0.22261900 0.56271217 163.59058237 |
| 26197 | | -0.48078586 0.33200516 -0.81155255 243.40917516 |
| 26198 | | Axis -0.55562941 0.77912031 0.29025419 |
| 26199 | | Axis point 211.38295724 0.00000000 71.01177343 |
| 26200 | | Rotation angle (degrees) 168.01774651 |
| 26201 | | Shift along axis 45.26267005 |
| 26202 | | |
| 26203 | | |
| 26204 | | > fitmap #48.13 inMap #47 |
| 26205 | | |
| 26206 | | Fit molecule copy of COPI_cyto_J4027_model M (#48.13) to map |
| 26207 | | J4021_J4027_composite_map.ccp4 (#47) using 2567 atoms |
| 26208 | | average map value = 6.181, steps = 48 |
| 26209 | | shifted from previous position = 0.0646 |
| 26210 | | rotated from previous position = 0.137 degrees |
| 26211 | | atoms outside contour = 1105, contour level = 5.8074 |
| 26212 | | |
| 26213 | | Position of copy of COPI_cyto_J4027_model M (#48.13) relative to |
| 26214 | | J4021_J4027_composite_map.ccp4 (#47) coordinates: |
| 26215 | | Matrix rotation and translation |
| 26216 | | -0.36546244 -0.91673285 -0.16136328 275.21816301 |
| 26217 | | -0.79822949 0.21948305 0.56094285 164.44314904 |
| 26218 | | -0.47881823 0.33380847 -0.81197600 242.98792253 |
| 26219 | | Axis -0.55679103 0.77820041 0.29049589 |
| 26220 | | Axis point 211.65663706 0.00000000 70.90505517 |
| 26221 | | Rotation angle (degrees) 168.23094482 |
| 26222 | | Shift along axis 45.31771373 |
| 26223 | | |
| 26224 | | |
| 26225 | | > fitmap #48.12 inMap #47 |
| 26226 | | |
| 26227 | | Fit molecule copy of COPI_cyto_J4027_model L (#48.12) to map |
| 26228 | | J4021_J4027_composite_map.ccp4 (#47) using 1420 atoms |
| 26229 | | average map value = 5.269, steps = 64 |
| 26230 | | shifted from previous position = 0.0436 |
| 26231 | | rotated from previous position = 0.124 degrees |
| 26232 | | atoms outside contour = 716, contour level = 5.8074 |
| 26233 | | |
| 26234 | | Position of copy of COPI_cyto_J4027_model L (#48.12) relative to |
| 26235 | | J4021_J4027_composite_map.ccp4 (#47) coordinates: |
| 26236 | | Matrix rotation and translation |
| 26237 | | -0.36575950 -0.91679867 -0.16031284 275.10067764 |
| 26238 | | -0.79603501 0.21891045 0.56427520 163.73853261 |
| 26239 | | -0.48223259 0.33400364 -0.80987241 243.20437721 |
| 26240 | | Axis -0.55646287 0.77793538 0.29183150 |
| 26241 | | Axis point 211.57205619 0.00000000 70.70465662 |
| 26242 | | Rotation angle (degrees) 168.05887425 |
| 26243 | | Shift along axis 45.26938091 |
| 26244 | | |
| 26245 | | |
| 26246 | | > fitmap #48.11 inMap #47 |
| 26247 | | |
| 26248 | | Fit molecule copy of COPI_cyto_J4027_model K (#48.11) to map |
| 26249 | | J4021_J4027_composite_map.ccp4 (#47) using 319 atoms |
| 26250 | | average map value = 6.544, steps = 680 |
| 26251 | | shifted from previous position = 34.4 |
| 26252 | | rotated from previous position = 55.2 degrees |
| 26253 | | atoms outside contour = 122, contour level = 5.8074 |
| 26254 | | |
| 26255 | | Position of copy of COPI_cyto_J4027_model K (#48.11) relative to |
| 26256 | | J4021_J4027_composite_map.ccp4 (#47) coordinates: |
| 26257 | | Matrix rotation and translation |
| 26258 | | 0.24248330 -0.95058432 0.19388478 218.57357673 |
| 26259 | | -0.33056267 0.10693534 0.93770633 73.53913137 |
| 26260 | | -0.91210207 -0.29146919 -0.28829766 267.39969287 |
| 26261 | | Axis -0.69605025 0.62629170 0.35110220 |
| 26262 | | Axis point 0.00000000 252.37218567 153.78684989 |
| 26263 | | Rotation angle (degrees) 117.99791989 |
| 26264 | | Shift along axis -12.19662658 |
| 26265 | | |
| 26266 | | |
| 26267 | | > ui tool show Matchmaker |
| 26268 | | |
| 26269 | | > matchmaker #48.11 to #49 |
| 26270 | | |
| 26271 | | Parameters |
| 26272 | | --- |
| 26273 | | Chain pairing | bb |
| 26274 | | Alignment algorithm | Needleman-Wunsch |
| 26275 | | Similarity matrix | BLOSUM-62 |
| 26276 | | SS fraction | 0.3 |
| 26277 | | Gap open (HH/SS/other) | 18/18/6 |
| 26278 | | Gap extend | 1 |
| 26279 | | SS matrix | | | H | S | O |
| 26280 | | ---|---|---|--- |
| 26281 | | H | 6 | -9 | -6 |
| 26282 | | S | | 6 | -6 |
| 26283 | | O | | | 4 |
| 26284 | | Iteration cutoff | 2 |
| 26285 | | |
| 26286 | | Matchmaker Model_for_composite_J4021_J4026, chain K (#49) with copy of |
| 26287 | | COPI_cyto_J4027_model K, chain K (#48.11), sequence alignment score = 192.1 |
| 26288 | | RMSD between 37 pruned atom pairs is 0.000 angstroms; (across all 37 pairs: |
| 26289 | | 0.000) |
| 26290 | | |
| 26291 | | |
| 26292 | | > fitmap #48.10 inMap #47 |
| 26293 | | |
| 26294 | | Fit molecule copy of COPI_cyto_J4027_model J (#48.10) to map |
| 26295 | | J4021_J4027_composite_map.ccp4 (#47) using 1112 atoms |
| 26296 | | average map value = 6.595, steps = 68 |
| 26297 | | shifted from previous position = 0.392 |
| 26298 | | rotated from previous position = 3.79 degrees |
| 26299 | | atoms outside contour = 449, contour level = 5.8074 |
| 26300 | | |
| 26301 | | Position of copy of COPI_cyto_J4027_model J (#48.10) relative to |
| 26302 | | J4021_J4027_composite_map.ccp4 (#47) coordinates: |
| 26303 | | Matrix rotation and translation |
| 26304 | | -0.36954265 -0.92395010 -0.09876469 269.14351053 |
| 26305 | | -0.79489549 0.25928902 0.54855298 162.57033903 |
| 26306 | | -0.48122698 0.28122133 -0.83026211 249.17503135 |
| 26307 | | Axis -0.55216997 0.78997075 0.26656057 |
| 26308 | | Axis point 209.80677258 0.00000000 74.24424921 |
| 26309 | | Rotation angle (degrees) 165.99103601 |
| 26310 | | Shift along axis 46.23308621 |
| 26311 | | |
| 26312 | | |
| 26313 | | > fitmap #48.9 inMap #47 |
| 26314 | | |
| 26315 | | Fit molecule copy of COPI_cyto_J4027_model I (#48.9) to map |
| 26316 | | J4021_J4027_composite_map.ccp4 (#47) using 1573 atoms |
| 26317 | | average map value = 5.861, steps = 68 |
| 26318 | | shifted from previous position = 1.24 |
| 26319 | | rotated from previous position = 3.45 degrees |
| 26320 | | atoms outside contour = 773, contour level = 5.8074 |
| 26321 | | |
| 26322 | | Position of copy of COPI_cyto_J4027_model I (#48.9) relative to |
| 26323 | | J4021_J4027_composite_map.ccp4 (#47) coordinates: |
| 26324 | | Matrix rotation and translation |
| 26325 | | -0.37973756 -0.89954761 -0.21590159 277.24792433 |
| 26326 | | -0.80457095 0.20595800 0.55699812 167.23271540 |
| 26327 | | -0.45657967 0.38522125 -0.80195986 236.87971276 |
| 26328 | | Axis -0.55310174 0.77495569 0.30581391 |
| 26329 | | Axis point 209.49101626 0.00000000 70.11224809 |
| 26330 | | Rotation angle (degrees) 171.06667095 |
| 26331 | | Shift along axis 48.69274569 |
| 26332 | | |
| 26333 | | |
| 26334 | | > fitmap #48.8 inMap #47 |
| 26335 | | |
| 26336 | | Fit molecule copy of COPI_cyto_J4027_model H (#48.8) to map |
| 26337 | | J4021_J4027_composite_map.ccp4 (#47) using 420 atoms |
| 26338 | | average map value = 6.824, steps = 56 |
| 26339 | | shifted from previous position = 3.38 |
| 26340 | | rotated from previous position = 2.53 degrees |
| 26341 | | atoms outside contour = 149, contour level = 5.8074 |
| 26342 | | |
| 26343 | | Position of copy of COPI_cyto_J4027_model H (#48.8) relative to |
| 26344 | | J4021_J4027_composite_map.ccp4 (#47) coordinates: |
| 26345 | | Matrix rotation and translation |
| 26346 | | -0.40542761 -0.90093167 -0.15475978 276.94991852 |
| 26347 | | -0.78307394 0.25494593 0.56727223 160.97102962 |
| 26348 | | -0.47161814 0.35117617 -0.80885823 241.79873195 |
| 26349 | | Axis -0.53858123 0.78971345 0.29373956 |
| 26350 | | Axis point 207.95662584 0.00000000 71.49993787 |
| 26351 | | Rotation angle (degrees) 168.42701468 |
| 26352 | | Shift along axis 48.98681208 |
| 26353 | | |
| 26354 | | |
| 26355 | | > fitmap #48.7 inMap #47 |
| 26356 | | |
| 26357 | | Fit molecule copy of COPI_cyto_J4027_model G (#48.7) to map |
| 26358 | | J4021_J4027_composite_map.ccp4 (#47) using 2302 atoms |
| 26359 | | average map value = 5.298, steps = 64 |
| 26360 | | shifted from previous position = 4.62 |
| 26361 | | rotated from previous position = 4.04 degrees |
| 26362 | | atoms outside contour = 1402, contour level = 5.8074 |
| 26363 | | |
| 26364 | | Position of copy of COPI_cyto_J4027_model G (#48.7) relative to |
| 26365 | | J4021_J4027_composite_map.ccp4 (#47) coordinates: |
| 26366 | | Matrix rotation and translation |
| 26367 | | -0.42560093 -0.89008522 -0.16313234 277.69917989 |
| 26368 | | -0.76548490 0.25798571 0.58947118 157.21311144 |
| 26369 | | -0.48259377 0.37575482 -0.79114574 238.50649670 |
| 26370 | | Axis -0.52893684 0.79065037 0.30837932 |
| 26371 | | Axis point 205.81643686 0.00000000 68.93017279 |
| 26372 | | Rotation angle (degrees) 168.34463133 |
| 26373 | | Shift along axis 50.96574868 |
| 26374 | | |
| 26375 | | |
| 26376 | | > fitmap #48.6 inMap #47 |
| 26377 | | |
| 26378 | | Fit molecule copy of COPI_cyto_J4027_model F (#48.6) to map |
| 26379 | | J4021_J4027_composite_map.ccp4 (#47) using 2400 atoms |
| 26380 | | average map value = 4.862, steps = 56 |
| 26381 | | shifted from previous position = 4.53 |
| 26382 | | rotated from previous position = 7.12 degrees |
| 26383 | | atoms outside contour = 1891, contour level = 5.8074 |
| 26384 | | |
| 26385 | | Position of copy of COPI_cyto_J4027_model F (#48.6) relative to |
| 26386 | | J4021_J4027_composite_map.ccp4 (#47) coordinates: |
| 26387 | | Matrix rotation and translation |
| 26388 | | -0.47631791 -0.86443647 -0.16084418 276.54476513 |
| 26389 | | -0.74836077 0.30252539 0.59028346 150.15879842 |
| 26390 | | -0.46160309 0.40153206 -0.79100860 234.60647851 |
| 26391 | | Axis -0.50526012 0.80508798 0.31071780 |
| 26392 | | Axis point 198.13646970 0.00000000 70.54602612 |
| 26393 | | Rotation angle (degrees) 169.23470102 |
| 26394 | | Shift along axis 54.06041328 |
| 26395 | | |
| 26396 | | |
| 26397 | | > fitmap #48.5 inMap #47 |
| 26398 | | |
| 26399 | | Fit molecule copy of COPI_cyto_J4027_model E (#48.5) to map |
| 26400 | | J4021_J4027_composite_map.ccp4 (#47) using 1876 atoms |
| 26401 | | average map value = 6.882, steps = 40 |
| 26402 | | shifted from previous position = 0.0401 |
| 26403 | | rotated from previous position = 0.0808 degrees |
| 26404 | | atoms outside contour = 679, contour level = 5.8074 |
| 26405 | | |
| 26406 | | Position of copy of COPI_cyto_J4027_model E (#48.5) relative to |
| 26407 | | J4021_J4027_composite_map.ccp4 (#47) coordinates: |
| 26408 | | Matrix rotation and translation |
| 26409 | | -0.36460635 -0.91721639 -0.16055006 274.96464339 |
| 26410 | | -0.79803633 0.21895894 0.56142232 164.31313284 |
| 26411 | | -0.47979187 0.33282292 -0.81180581 243.25738221 |
| 26412 | | Axis -0.55710084 0.77799805 0.29044395 |
| 26413 | | Axis point 211.64530256 0.00000000 70.95305334 |
| 26414 | | Rotation angle (degrees) 168.16062068 |
| 26415 | | Shift along axis 45.30489541 |
| 26416 | | |
| 26417 | | |
| 26418 | | > fitmap #48.4 inMap #47 |
| 26419 | | |
| 26420 | | Fit molecule copy of COPI_cyto_J4027_model D (#48.4) to map |
| 26421 | | J4021_J4027_composite_map.ccp4 (#47) using 4745 atoms |
| 26422 | | average map value = 6.326, steps = 80 |
| 26423 | | shifted from previous position = 0.232 |
| 26424 | | rotated from previous position = 0.637 degrees |
| 26425 | | atoms outside contour = 1901, contour level = 5.8074 |
| 26426 | | |
| 26427 | | Position of copy of COPI_cyto_J4027_model D (#48.4) relative to |
| 26428 | | J4021_J4027_composite_map.ccp4 (#47) coordinates: |
| 26429 | | Matrix rotation and translation |
| 26430 | | -0.36403643 -0.91927407 -0.14970864 274.32296003 |
| 26431 | | -0.79661223 0.22402751 0.56144514 163.63243958 |
| 26432 | | -0.48258310 0.32364622 -0.81385913 244.76587458 |
| 26433 | | Axis -0.55679826 0.77941446 0.28720862 |
| 26434 | | Axis point 211.67256763 0.00000000 71.48567291 |
| 26435 | | Rotation angle (degrees) 167.67003358 |
| 26436 | | Shift along axis 45.09381034 |
| 26437 | | |
| 26438 | | |
| 26439 | | > fitmap #48.3 inMap #47 |
| 26440 | | |
| 26441 | | Fit molecule copy of COPI_cyto_J4027_model C (#48.3) to map |
| 26442 | | J4021_J4027_composite_map.ccp4 (#47) using 1612 atoms |
| 26443 | | average map value = 6.318, steps = 80 |
| 26444 | | shifted from previous position = 0.228 |
| 26445 | | rotated from previous position = 1.41 degrees |
| 26446 | | atoms outside contour = 653, contour level = 5.8074 |
| 26447 | | |
| 26448 | | Position of copy of COPI_cyto_J4027_model C (#48.3) relative to |
| 26449 | | J4021_J4027_composite_map.ccp4 (#47) coordinates: |
| 26450 | | Matrix rotation and translation |
| 26451 | | -0.38173446 -0.91224090 -0.14864510 275.71683603 |
| 26452 | | -0.78322797 0.23388065 0.57606754 161.04568217 |
| 26453 | | -0.49074715 0.33632783 -0.80377288 243.57494299 |
| 26454 | | Axis -0.54833896 0.78246479 0.29508175 |
| 26455 | | Axis point 210.53404344 0.00000000 70.11714477 |
| 26456 | | Rotation angle (degrees) 167.37286555 |
| 26457 | | Shift along axis 46.70081412 |
| 26458 | | |
| 26459 | | |
| 26460 | | > fitmap #48.2 inMap #47 |
| 26461 | | |
| 26462 | | Fit molecule copy of COPI_cyto_J4027_model B (#48.2) to map |
| 26463 | | J4021_J4027_composite_map.ccp4 (#47) using 2426 atoms |
| 26464 | | average map value = 7.483, steps = 48 |
| 26465 | | shifted from previous position = 0.0329 |
| 26466 | | rotated from previous position = 0.105 degrees |
| 26467 | | atoms outside contour = 734, contour level = 5.8074 |
| 26468 | | |
| 26469 | | Position of copy of COPI_cyto_J4027_model B (#48.2) relative to |
| 26470 | | J4021_J4027_composite_map.ccp4 (#47) coordinates: |
| 26471 | | Matrix rotation and translation |
| 26472 | | -0.36430973 -0.91755945 -0.15925792 274.83521303 |
| 26473 | | -0.79785551 0.21931761 0.56153930 164.30315268 |
| 26474 | | -0.48031762 0.33163904 -0.81197940 243.32909311 |
| 26475 | | Axis -0.55716005 0.77808367 0.29010081 |
| 26476 | | Axis point 211.66844489 0.00000000 70.92695219 |
| 26477 | | Rotation angle (degrees) 168.09354740 |
| 26478 | | Shift along axis 45.30436790 |
| 26479 | | |
| 26480 | | |
| 26481 | | > fitmap #48.1 inMap #47 |
| 26482 | | |
| 26483 | | Fit molecule copy of COPI_cyto_J4027_model A (#48.1) to map |
| 26484 | | J4021_J4027_composite_map.ccp4 (#47) using 2590 atoms |
| 26485 | | average map value = 7.767, steps = 64 |
| 26486 | | shifted from previous position = 0.0157 |
| 26487 | | rotated from previous position = 0.0377 degrees |
| 26488 | | atoms outside contour = 732, contour level = 5.8074 |
| 26489 | | |
| 26490 | | Position of copy of COPI_cyto_J4027_model A (#48.1) relative to |
| 26491 | | J4021_J4027_composite_map.ccp4 (#47) coordinates: |
| 26492 | | Matrix rotation and translation |
| 26493 | | -0.36433385 -0.91729312 -0.16073020 274.99544480 |
| 26494 | | -0.79752965 0.21821532 0.56243093 164.22320875 |
| 26495 | | -0.48084022 0.33309973 -0.81107168 243.29313210 |
| 26496 | | Axis -0.55718331 0.77773961 0.29097743 |
| 26497 | | Axis point 211.71070241 0.00000000 70.85027180 |
| 26498 | | Rotation angle (degrees) 168.12395214 |
| 26499 | | Shift along axis 45.29283319 |
| 26500 | | |
| 26501 | | |
| 26502 | | > combine #48.1 #48.2 #48.3 #48.4 #48.5 #48.6 #48.7 #48.8 #48.9 #48.10 #48.11 |
| 26503 | | > #48.12 #48.13 #48.14 #48.15 |
| 26504 | | |
| 26505 | | > rename #50 Model_for_composite_J4021_J4027 |
| 26506 | | |
| 26507 | | > close #48 |
| 26508 | | |
| 26509 | | > show #!49 models |
| 26510 | | |
| 26511 | | > hide #!49 models |
| 26512 | | |
| 26513 | | > hide #!50 models |
| 26514 | | |
| 26515 | | > save composite_model_J4021_J4026.pdb #49 |
| 26516 | | |
| 26517 | | > save composite_model_J4021_J4027.pdb #50 |
| 26518 | | |
| 26519 | | > save "/Users/becca/Desktop/Postdoc/COPI notebooks and |
| 26520 | | > resources/Processing/W20_MM_cyto_COPI/20250707_W20_COPI_cyto_alignments.cxs" |
| 26521 | | |
| 26522 | | > hide #!47 models |
| 26523 | | |
| 26524 | | > show #!1 models |
| 26525 | | |
| 26526 | | > color #1 #a9a9a9bf models |
| 26527 | | |
| 26528 | | > ui tool show "Surface Color" |
| 26529 | | |
| 26530 | | > ui tool show "Color Zone" |
| 26531 | | |
| 26532 | | > color zone #1 near #35 distance 6 |
| 26533 | | |
| 26534 | | > hide #!1 models |
| 26535 | | |
| 26536 | | > show #!1 models |
| 26537 | | |
| 26538 | | > select #35/K |
| 26539 | | |
| 26540 | | 319 atoms, 320 bonds, 37 residues, 1 model selected |
| 26541 | | |
| 26542 | | > hide #!1 models |
| 26543 | | |
| 26544 | | > show #!35 models |
| 26545 | | |
| 26546 | | > select #35/M |
| 26547 | | |
| 26548 | | 4552 atoms, 4624 bonds, 582 residues, 1 model selected |
| 26549 | | |
| 26550 | | > color sel gammav3 |
| 26551 | | |
| 26552 | | > select add #35 |
| 26553 | | |
| 26554 | | 27347 atoms, 27860 bonds, 1 pseudobond, 3435 residues, 2 models selected |
| 26555 | | |
| 26556 | | > hide #!35 models |
| 26557 | | |
| 26558 | | > select subtract #35 |
| 26559 | | |
| 26560 | | Nothing selected |
| 26561 | | |
| 26562 | | > show #!36 models |
| 26563 | | |
| 26564 | | > hide #!36 models |
| 26565 | | |
| 26566 | | > show #!37 models |
| 26567 | | |
| 26568 | | > select #36/M |
| 26569 | | |
| 26570 | | 4552 atoms, 4624 bonds, 582 residues, 1 model selected |
| 26571 | | |
| 26572 | | > color sel gammav3 |
| 26573 | | |
| 26574 | | > select #37/M |
| 26575 | | |
| 26576 | | 4552 atoms, 4624 bonds, 582 residues, 1 model selected |
| 26577 | | |
| 26578 | | > color sel gammav3 |
| 26579 | | |
| 26580 | | > select add #37 |
| 26581 | | |
| 26582 | | 27347 atoms, 27860 bonds, 1 pseudobond, 3435 residues, 2 models selected |
| 26583 | | |
| 26584 | | > select subtract #37 |
| 26585 | | |
| 26586 | | Nothing selected |
| 26587 | | |
| 26588 | | > hide #!37 models |
| 26589 | | |
| 26590 | | > show #!38 models |
| 26591 | | |
| 26592 | | > select #38/M |
| 26593 | | |
| 26594 | | 4552 atoms, 4624 bonds, 582 residues, 1 model selected |
| 26595 | | |
| 26596 | | > color sel gammav3 |
| 26597 | | |
| 26598 | | > select #39/m |
| 26599 | | |
| 26600 | | 4552 atoms, 4624 bonds, 582 residues, 1 model selected |
| 26601 | | |
| 26602 | | > color sel gammav3 |
| 26603 | | |
| 26604 | | > select add #39 |
| 26605 | | |
| 26606 | | 27347 atoms, 27860 bonds, 1 pseudobond, 3435 residues, 2 models selected |
| 26607 | | |
| 26608 | | > select subtract #39 |
| 26609 | | |
| 26610 | | Nothing selected |
| 26611 | | |
| 26612 | | > hide #!38 models |
| 26613 | | |
| 26614 | | > show #!39 models |
| 26615 | | |
| 26616 | | > hide #!39 models |
| 26617 | | |
| 26618 | | > show #!40 models |
| 26619 | | |
| 26620 | | > select #40/m |
| 26621 | | |
| 26622 | | 2567 atoms, 2609 bonds, 330 residues, 1 model selected |
| 26623 | | |
| 26624 | | > color sel gammav3 |
| 26625 | | |
| 26626 | | > select #40/n |
| 26627 | | |
| 26628 | | 930 atoms, 941 bonds, 118 residues, 1 model selected |
| 26629 | | |
| 26630 | | > color sel gammav3 |
| 26631 | | |
| 26632 | | > select #40/o |
| 26633 | | |
| 26634 | | 1064 atoms, 1080 bonds, 1 pseudobond, 135 residues, 2 models selected |
| 26635 | | |
| 26636 | | > color sel gammav3 |
| 26637 | | |
| 26638 | | > select #41/m |
| 26639 | | |
| 26640 | | 2567 atoms, 2609 bonds, 330 residues, 1 model selected |
| 26641 | | |
| 26642 | | > hide #!40 models |
| 26643 | | |
| 26644 | | > show #!41 models |
| 26645 | | |
| 26646 | | > color sel gammav3 |
| 26647 | | |
| 26648 | | > select #41/n |
| 26649 | | |
| 26650 | | 930 atoms, 941 bonds, 118 residues, 1 model selected |
| 26651 | | |
| 26652 | | > color sel gammav3 |
| 26653 | | |
| 26654 | | > select #41/o |
| 26655 | | |
| 26656 | | 1064 atoms, 1080 bonds, 1 pseudobond, 135 residues, 2 models selected |
| 26657 | | |
| 26658 | | > color sel gammav3 |
| 26659 | | |
| 26660 | | > select add #41 |
| 26661 | | |
| 26662 | | 27356 atoms, 27866 bonds, 2 pseudobonds, 3436 residues, 2 models selected |
| 26663 | | |
| 26664 | | > select subtract #41 |
| 26665 | | |
| 26666 | | Nothing selected |
| 26667 | | |
| 26668 | | > hide #!41 models |
| 26669 | | |
| 26670 | | > show #!42 models |
| 26671 | | |
| 26672 | | > select #42/m |
| 26673 | | |
| 26674 | | 4552 atoms, 4624 bonds, 582 residues, 1 model selected |
| 26675 | | |
| 26676 | | > color sel gammav3 |
| 26677 | | |
| 26678 | | > select add #42 |
| 26679 | | |
| 26680 | | 27347 atoms, 27860 bonds, 1 pseudobond, 3435 residues, 2 models selected |
| 26681 | | |
| 26682 | | > select subtract #42 |
| 26683 | | |
| 26684 | | Nothing selected |
| 26685 | | |
| 26686 | | > hide #!42 models |
| 26687 | | |
| 26688 | | > show #!43 models |
| 26689 | | |
| 26690 | | > select #43/m |
| 26691 | | |
| 26692 | | 2618 atoms, 2660 bonds, 337 residues, 1 model selected |
| 26693 | | |
| 26694 | | > color sel gammav3 |
| 26695 | | |
| 26696 | | > select #43/n |
| 26697 | | |
| 26698 | | 878 atoms, 889 bonds, 111 residues, 1 model selected |
| 26699 | | |
| 26700 | | > color sel gammav3 |
| 26701 | | |
| 26702 | | > select #43/o |
| 26703 | | |
| 26704 | | 1064 atoms, 1080 bonds, 1 pseudobond, 135 residues, 2 models selected |
| 26705 | | |
| 26706 | | > color sel gammav3 |
| 26707 | | |
| 26708 | | > select add #43 |
| 26709 | | |
| 26710 | | 27355 atoms, 27865 bonds, 2 pseudobonds, 3436 residues, 2 models selected |
| 26711 | | |
| 26712 | | > hide #!43 models |
| 26713 | | |
| 26714 | | > select subtract #43 |
| 26715 | | |
| 26716 | | Nothing selected |
| 26717 | | |
| 26718 | | > show #!44 models |
| 26719 | | |
| 26720 | | > select #44/o |
| 26721 | | |
| 26722 | | 1064 atoms, 1080 bonds, 1 pseudobond, 135 residues, 2 models selected |
| 26723 | | |
| 26724 | | > color sel gammav3 |
| 26725 | | |
| 26726 | | > select #44/n |
| 26727 | | |
| 26728 | | 878 atoms, 889 bonds, 111 residues, 1 model selected |
| 26729 | | |
| 26730 | | > color sel gammav3 |
| 26731 | | |
| 26732 | | > select #44/m |
| 26733 | | |
| 26734 | | 2618 atoms, 2660 bonds, 337 residues, 1 model selected |
| 26735 | | |
| 26736 | | > color sel gammav3 |
| 26737 | | |
| 26738 | | > select add #44 |
| 26739 | | |
| 26740 | | 27355 atoms, 27865 bonds, 2 pseudobonds, 3436 residues, 2 models selected |
| 26741 | | |
| 26742 | | > hide #!44 models |
| 26743 | | |
| 26744 | | > select subtract #44 |
| 26745 | | |
| 26746 | | Nothing selected |
| 26747 | | |
| 26748 | | > show #!45 models |
| 26749 | | |
| 26750 | | > select #45/m |
| 26751 | | |
| 26752 | | 4552 atoms, 4624 bonds, 582 residues, 1 model selected |
| 26753 | | |
| 26754 | | > color sel gammav3 |
| 26755 | | |
| 26756 | | > select add #45 |
| 26757 | | |
| 26758 | | 27347 atoms, 27860 bonds, 1 pseudobond, 3435 residues, 2 models selected |
| 26759 | | |
| 26760 | | > select subtract #45 |
| 26761 | | |
| 26762 | | Nothing selected |
| 26763 | | |
| 26764 | | > hide #!45 models |
| 26765 | | |
| 26766 | | > show #!1 models |
| 26767 | | |
| 26768 | | > color zone #1 near #35 distance 6 |
| 26769 | | |
| 26770 | | > volume #1 level 0.1319 |
| 26771 | | |
| 26772 | | > color zone #1 near #35 distance 6.1 |
| 26773 | | |
| 26774 | | > color zone #1 near #35 distance 6.2 |
| 26775 | | |
| 26776 | | > color zone #1 near #35 distance 6.3 |
| 26777 | | |
| 26778 | | > color zone #1 near #35 distance 6.4 |
| 26779 | | |
| 26780 | | > color zone #1 near #35 distance 6.5 |
| 26781 | | |
| 26782 | | > color zone #1 near #35 distance 6.6 |
| 26783 | | |
| 26784 | | > color zone #1 near #35 distance 6.7 |
| 26785 | | |
| 26786 | | > color zone #1 near #35 distance 9 |
| 26787 | | |
| 26788 | | [Repeated 1 time(s)] |
| 26789 | | |
| 26790 | | > volume #1 level 0.1857 |
| 26791 | | |
| 26792 | | > view view1 |
| 26793 | | |
| 26794 | | Expected an objects specifier or a view name or a keyword |
| 26795 | | |
| 26796 | | > view name view1 |
| 26797 | | |
| 26798 | | > rotate 90 |
| 26799 | | |
| 26800 | | Unknown command: rotate 90 |
| 26801 | | |
| 26802 | | > turn 90 |
| 26803 | | |
| 26804 | | Expected an axis vector or a keyword |
| 26805 | | |
| 26806 | | > turn x 90 |
| 26807 | | |
| 26808 | | [Repeated 3 time(s)] |
| 26809 | | |
| 26810 | | > turn y 90 |
| 26811 | | |
| 26812 | | [Repeated 3 time(s)] |
| 26813 | | |
| 26814 | | > turn y -90 |
| 26815 | | |
| 26816 | | > view name view2 |
| 26817 | | |
| 26818 | | > turn y 90 |
| 26819 | | |
| 26820 | | > view view2 |
| 26821 | | |
| 26822 | | > turn y 180 |
| 26823 | | |
| 26824 | | > view name view3 |
| 26825 | | |
| 26826 | | > view view3 |
| 26827 | | |
| 26828 | | > turn y 180 |
| 26829 | | |
| 26830 | | > view name view4 |
| 26831 | | |
| 26832 | | [Repeated 1 time(s)] |
| 26833 | | |
| 26834 | | > view view3 |
| 26835 | | |
| 26836 | | > view name view3 |
| 26837 | | |
| 26838 | | > view name view5 |
| 26839 | | |
| 26840 | | > lighting soft |
| 26841 | | |
| 26842 | | > set silhouetteWidth 1.5 |
| 26843 | | |
| 26844 | | > ui tool show "Side View" |
| 26845 | | |
| 26846 | | > save |
| 26847 | | > /Users/becca/Desktop/Postdoc/Figures/20250707_Poster/J3623_consensus_view5.png |
| 26848 | | > width 823 height 607 supersample 3 transparentBackground true |
| 26849 | | |
| 26850 | | > view view3 |
| 26851 | | |
| 26852 | | > save |
| 26853 | | > /Users/becca/Desktop/Postdoc/Figures/20250707_Poster/J3623_consensus_view3.png |
| 26854 | | > width 823 height 607 supersample 3 transparentBackground true |
| 26855 | | |
| 26856 | | > view view4 |
| 26857 | | |
| 26858 | | > save |
| 26859 | | > /Users/becca/Desktop/Postdoc/Figures/20250707_Poster/J3623_consensus_view4.png |
| 26860 | | > width 823 height 607 supersample 3 transparentBackground true |
| 26861 | | |
| 26862 | | > save "/Users/becca/Desktop/Postdoc/COPI notebooks and |
| 26863 | | > resources/Processing/W20_MM_cyto_COPI/20250707_W20_COPI_cyto_alignments.cxs" |
| 26864 | | |
| 26865 | | > open "/Users/becca/Desktop/Postdoc/COPI notebooks and |
| 26866 | | > resources/Processing/W20_MM_cyto_COPI/cryosparc_P126_J4479_map_locres_J3623.mrc" |
| 26867 | | |
| 26868 | | Opened cryosparc_P126_J4479_map_locres_J3623.mrc as #48, grid size |
| 26869 | | 240,240,240, pixel 1.19, shown at level 11.6, step 1, values float32 |
| 26870 | | |
| 26871 | | > ui tool show "Surface Color" |
| 26872 | | |
| 26873 | | > color sample #1.1 map #48 palette #ff0000:#ffffff:#0000ff outsideColor |
| 26874 | | > #808080 |
| 26875 | | |
| 26876 | | Map values for surface "surface": minimum 2.577, mean 4.107, maximum 7.766 |
| 26877 | | |
| 26878 | | > color sample #1.1 map #48 palette 2,#ff0000:4,#ffffff:10,#0000ff |
| 26879 | | > outsideColor #808080 |
| 26880 | | |
| 26881 | | Map values for surface "surface": minimum 2.577, mean 4.107, maximum 7.766 |
| 26882 | | |
| 26883 | | > color sample #1.1 map #48 palette 3,#ff0000:4,#ffffff:10,#0000ff |
| 26884 | | > outsideColor #808080 |
| 26885 | | |
| 26886 | | Map values for surface "surface": minimum 2.577, mean 4.107, maximum 7.766 |
| 26887 | | |
| 26888 | | > color sample #1.1 map #48 palette 3,#ff0000:5,#ffffff:8,#0000ff outsideColor |
| 26889 | | > #808080 |
| 26890 | | |
| 26891 | | Map values for surface "surface": minimum 2.577, mean 4.107, maximum 7.766 |
| 26892 | | |
| 26893 | | > color sample #1.1 map #48 palette 4,#ff0000:6,#ffffff:8,#0000ff outsideColor |
| 26894 | | > #808080 |
| 26895 | | |
| 26896 | | Map values for surface "surface": minimum 2.577, mean 4.107, maximum 7.766 |
| 26897 | | |
| 26898 | | > color single #1.1 |
| 26899 | | |
| 26900 | | > key red-white-blue :4 :6 :8 showTool true |
| 26901 | | |
| 26902 | | > ui mousemode right "color key" |
| 26903 | | |
| 26904 | | > color sample #1.1 map #48 palette 2.5,#ff0000:6,#ffffff:8,#0000ff |
| 26905 | | > outsideColor #808080 |
| 26906 | | |
| 26907 | | Map values for surface "surface": minimum 2.577, mean 4.107, maximum 7.766 |
| 26908 | | |
| 26909 | | > key red-white-blue :2.5 :6.0 :8.0 showTool true |
| 26910 | | |
| 26911 | | > color sample #1.1 map #48 palette 2.5,#ff0000:4,#ffffff:8,#0000ff |
| 26912 | | > outsideColor #808080 |
| 26913 | | |
| 26914 | | Map values for surface "surface": minimum 2.577, mean 4.107, maximum 7.766 |
| 26915 | | |
| 26916 | | > key red-white-blue :2.5 :4.0 :8.0 showTool true |
| 26917 | | |
| 26918 | | > color sample #1.1 map #48 palette 2.5,#ff0000:4,#ffffff:6.5,#0000ff |
| 26919 | | > outsideColor #808080 |
| 26920 | | |
| 26921 | | Map values for surface "surface": minimum 2.577, mean 4.107, maximum 7.766 |
| 26922 | | |
| 26923 | | > key red-white-blue :2.5 :4.0 :6.5 showTool true |
| 26924 | | |
| 26925 | | > color single #1.1 |
| 26926 | | |
| 26927 | | > color sample #1.1 map #48 palette 2.5,#ff0000:4,#ffffff:6.5,#0000ff |
| 26928 | | > outsideColor #808080 |
| 26929 | | |
| 26930 | | Map values for surface "surface": minimum 2.577, mean 4.107, maximum 7.766 |
| 26931 | | |
| 26932 | | > color sample #1.1 map #48 palette 3,#ff0000:4,#ffffff:6.5,#0000ff |
| 26933 | | > outsideColor #808080 |
| 26934 | | |
| 26935 | | Map values for surface "surface": minimum 2.577, mean 4.107, maximum 7.766 |
| 26936 | | |
| 26937 | | > key red-white-blue :3.0 :4.0 :6.5 showTool true |
| 26938 | | |
| 26939 | | > color sample #1.1 map #48 palette 3,#ff0000:4.5,#ffffff:6.5,#0000ff |
| 26940 | | > outsideColor #808080 |
| 26941 | | |
| 26942 | | Map values for surface "surface": minimum 2.577, mean 4.107, maximum 7.766 |
| | 2424 | [deleted to fit within ticket limits] |