1925 | | > open "/Users/becca/Desktop/Postdoc/COPI notebooks and |
1926 | | > resources/Processing/W20_MM_cyto_COPI/W20_J4021_005_volume_map_bprime_local.mrc" |
1927 | | |
1928 | | Opened W20_J4021_005_volume_map_bprime_local.mrc as #13, grid size |
1929 | | 240,240,240, pixel 1.19, shown at level 0.26, step 1, values float32 |
1930 | | |
1931 | | > open "/Users/becca/Desktop/Postdoc/COPI notebooks and |
1932 | | > resources/Processing/W20_MM_cyto_COPI/W20_J4021_005_volume_map_sharp_bprime_local.mrc" |
1933 | | |
1934 | | Opened W20_J4021_005_volume_map_sharp_bprime_local.mrc as #14, grid size |
1935 | | 240,240,240, pixel 1.19, shown at level 0.291, step 1, values float32 |
1936 | | |
1937 | | > open "/Users/becca/Desktop/Postdoc/COPI notebooks and |
1938 | | > resources/Processing/W20_MM_cyto_COPI/W20_J4022_005_volume_map_bprime_local.mrc" |
1939 | | |
1940 | | Opened W20_J4022_005_volume_map_bprime_local.mrc as #15, grid size |
1941 | | 240,240,240, pixel 1.19, shown at level 0.256, step 1, values float32 |
1942 | | |
1943 | | > open "/Users/becca/Desktop/Postdoc/COPI notebooks and |
1944 | | > resources/Processing/W20_MM_cyto_COPI/W20_J4022_005_volume_map_sharp_bprime_local.mrc" |
1945 | | |
1946 | | Opened W20_J4022_005_volume_map_sharp_bprime_local.mrc as #16, grid size |
1947 | | 240,240,240, pixel 1.19, shown at level 0.291, step 1, values float32 |
1948 | | |
1949 | | > open "/Users/becca/Desktop/Postdoc/COPI notebooks and |
1950 | | > resources/Processing/W20_MM_cyto_COPI/W20_J4023_003_volume_map_bprime_local.mrc" |
1951 | | |
1952 | | Opened W20_J4023_003_volume_map_bprime_local.mrc as #17, grid size |
1953 | | 240,240,240, pixel 1.19, shown at level 0.26, step 1, values float32 |
1954 | | |
1955 | | > open "/Users/becca/Desktop/Postdoc/COPI notebooks and |
1956 | | > resources/Processing/W20_MM_cyto_COPI/W20_J4023_003_volume_map_sharp_bprime_local.mrc" |
1957 | | |
1958 | | Opened W20_J4023_003_volume_map_sharp_bprime_local.mrc as #18, grid size |
1959 | | 240,240,240, pixel 1.19, shown at level 0.321, step 1, values float32 |
1960 | | |
1961 | | > open "/Users/becca/Desktop/Postdoc/COPI notebooks and |
1962 | | > resources/Processing/W20_MM_cyto_COPI/W20_J4024_005_volume_map_bprime_local.mrc" |
1963 | | |
1964 | | Opened W20_J4024_005_volume_map_bprime_local.mrc as #19, grid size |
1965 | | 240,240,240, pixel 1.19, shown at level 0.256, step 1, values float32 |
1966 | | |
1967 | | > open "/Users/becca/Desktop/Postdoc/COPI notebooks and |
1968 | | > resources/Processing/W20_MM_cyto_COPI/W20_J4024_005_volume_map_sharp_bprime_local.mrc" |
1969 | | |
1970 | | Opened W20_J4024_005_volume_map_sharp_bprime_local.mrc as #20, grid size |
1971 | | 240,240,240, pixel 1.19, shown at level 0.291, step 1, values float32 |
1972 | | |
1973 | | > open "/Users/becca/Desktop/Postdoc/COPI notebooks and |
1974 | | > resources/Processing/W20_MM_cyto_COPI/W20_J4025_004_volume_map_bprime_local.mrc" |
1975 | | |
1976 | | Opened W20_J4025_004_volume_map_bprime_local.mrc as #21, grid size |
1977 | | 240,240,240, pixel 1.19, shown at level 0.261, step 1, values float32 |
1978 | | |
1979 | | > open "/Users/becca/Desktop/Postdoc/COPI notebooks and |
1980 | | > resources/Processing/W20_MM_cyto_COPI/W20_J4025_004_volume_map_sharp_bprime_local.mrc" |
1981 | | |
1982 | | Opened W20_J4025_004_volume_map_sharp_bprime_local.mrc as #22, grid size |
1983 | | 240,240,240, pixel 1.19, shown at level 0.347, step 1, values float32 |
1984 | | |
1985 | | > open "/Users/becca/Desktop/Postdoc/COPI notebooks and |
1986 | | > resources/Processing/W20_MM_cyto_COPI/W20_J4026_004_volume_map_gammazeta_local.mrc" |
1987 | | |
1988 | | Opened W20_J4026_004_volume_map_gammazeta_local.mrc as #23, grid size |
1989 | | 240,240,240, pixel 1.19, shown at level 0.26, step 1, values float32 |
1990 | | |
1991 | | > open "/Users/becca/Desktop/Postdoc/COPI notebooks and |
1992 | | > resources/Processing/W20_MM_cyto_COPI/W20_J4026_004_volume_map_sharp_gammazeta_local.mrc" |
1993 | | |
1994 | | Opened W20_J4026_004_volume_map_sharp_gammazeta_local.mrc as #24, grid size |
1995 | | 240,240,240, pixel 1.19, shown at level 0.272, step 1, values float32 |
1996 | | |
1997 | | > open "/Users/becca/Desktop/Postdoc/COPI notebooks and |
1998 | | > resources/Processing/W20_MM_cyto_COPI/W20_J4027_005_volume_map_gammazeta_local.mrc" |
1999 | | |
2000 | | Opened W20_J4027_005_volume_map_gammazeta_local.mrc as #25, grid size |
2001 | | 240,240,240, pixel 1.19, shown at level 0.253, step 1, values float32 |
2002 | | |
2003 | | > open "/Users/becca/Desktop/Postdoc/COPI notebooks and |
2004 | | > resources/Processing/W20_MM_cyto_COPI/W20_J4027_005_volume_map_sharp_gammazeta_local.mrc" |
2005 | | |
2006 | | Opened W20_J4027_005_volume_map_sharp_gammazeta_local.mrc as #26, grid size |
2007 | | 240,240,240, pixel 1.19, shown at level 0.268, step 1, values float32 |
2008 | | |
2009 | | > open "/Users/becca/Desktop/Postdoc/COPI notebooks and |
2010 | | > resources/Processing/W20_MM_cyto_COPI/W20_J4028_004_volume_map_gammazeta_local.mrc" |
2011 | | |
2012 | | Opened W20_J4028_004_volume_map_gammazeta_local.mrc as #27, grid size |
2013 | | 240,240,240, pixel 1.19, shown at level 0.255, step 1, values float32 |
2014 | | |
2015 | | > open "/Users/becca/Desktop/Postdoc/COPI notebooks and |
2016 | | > resources/Processing/W20_MM_cyto_COPI/W20_J4028_004_volume_map_sharp_gammazeta_local.mrc" |
2017 | | |
2018 | | Opened W20_J4028_004_volume_map_sharp_gammazeta_local.mrc as #28, grid size |
2019 | | 240,240,240, pixel 1.19, shown at level 0.269, step 1, values float32 |
2020 | | |
2021 | | > hide #!28 models |
2022 | | |
2023 | | > hide #!27 models |
2024 | | |
2025 | | > hide #!26 models |
2026 | | |
2027 | | > hide #!25 models |
2028 | | |
2029 | | > hide #!24 models |
2030 | | |
2031 | | > hide #!23 models |
2032 | | |
2033 | | > hide #!22 models |
2034 | | |
2035 | | > hide #!21 models |
2036 | | |
2037 | | > hide #!20 models |
2038 | | |
2039 | | > hide #!19 models |
2040 | | |
2041 | | > hide #!18 models |
2042 | | |
2043 | | > hide #!17 models |
2044 | | |
2045 | | > hide #!16 models |
2046 | | |
2047 | | > hide #!15 models |
2048 | | |
2049 | | > hide #!14 models |
2050 | | |
2051 | | > hide #!13 models |
2052 | | |
2053 | | > hide #!12 models |
2054 | | |
2055 | | > hide #!11 models |
2056 | | |
2057 | | > hide #!10 models |
2058 | | |
2059 | | > hide #!9 models |
2060 | | |
2061 | | > hide #!8 models |
2062 | | |
2063 | | > hide #!7 models |
2064 | | |
2065 | | > hide #!6 models |
2066 | | |
2067 | | > hide #!5 models |
2068 | | |
2069 | | > hide #!4 models |
2070 | | |
2071 | | > hide #!3 models |
2072 | | |
2073 | | > hide #!2 models |
2074 | | |
2075 | | > show #!2 models |
2076 | | |
2077 | | > hide #!2 models |
2078 | | |
2079 | | > hide #!1 models |
2080 | | |
2081 | | > show #!1 models |
2082 | | |
2083 | | > show #!2 models |
2084 | | |
2085 | | > hide #!2 models |
2086 | | |
2087 | | > hide #!1 models |
2088 | | |
2089 | | > show #!3 models |
2090 | | |
2091 | | > show #!1 models |
2092 | | |
2093 | | > select add #3 |
2094 | | |
2095 | | 2 models selected |
2096 | | |
2097 | | > view matrix models #3,1,0,0,11.802,0,1,0,1.1649,0,0,1,22.807 |
2098 | | |
2099 | | > ui mousemode right "translate selected models" |
2100 | | |
2101 | | > ui mousemode right "rotate selected models" |
2102 | | |
2103 | | > view matrix models |
2104 | | > #3,-0.42058,-0.90712,0.015978,332.41,-0.80828,0.38263,0.44752,138.26,-0.41207,0.1753,-0.89413,312.09 |
2105 | | |
2106 | | > fitmap #3 inMap #1 |
2107 | | |
2108 | | Fit map |
2109 | | W20_J3985_004_volume_map_csparc_refine_of_relion_job121_bprime_mask.mrc in map |
2110 | | W20_J3623_consensus_structure_sharp.mrc using 138102 points |
2111 | | correlation = 0.9343, correlation about mean = 0.6786, overlap = 2.406e+04 |
2112 | | steps = 156, shift = 6.42, angle = 18.1 degrees |
2113 | | |
2114 | | Position of |
2115 | | W20_J3985_004_volume_map_csparc_refine_of_relion_job121_bprime_mask.mrc (#3) |
2116 | | relative to W20_J3623_consensus_structure_sharp.mrc (#1) coordinates: |
2117 | | Matrix rotation and translation |
2118 | | -0.34036295 -0.91876592 -0.20005563 354.03277644 |
2119 | | -0.77656396 0.15469587 0.61075167 146.14319511 |
2120 | | -0.53019004 0.36323323 -0.76613324 277.46223631 |
2121 | | Axis -0.56713702 0.75643433 0.32582620 |
2122 | | Axis point 252.05264579 0.00000000 81.40495015 |
2123 | | Rotation angle (degrees) 167.39564073 |
2124 | | Shift along axis 0.16710354 |
2125 | | |
2126 | | |
2127 | | > select subtract #3 |
2128 | | |
2129 | | Nothing selected |
2130 | | |
2131 | | > hide #!3 models |
2132 | | |
2133 | | > show #!3 models |
2134 | | |
2135 | | > hide #!3 models |
2136 | | |
2137 | | > show #!4 models |
2138 | | |
2139 | | > select add #4 |
2140 | | |
2141 | | 2 models selected |
2142 | | |
2143 | | > view matrix models |
2144 | | > #4,-0.16847,-0.98434,-0.051916,308.16,-0.77209,0.099036,0.62775,149.29,-0.61277,0.14584,-0.77668,305.19 |
2145 | | |
2146 | | > ui mousemode right "translate selected models" |
2147 | | |
2148 | | > view matrix models |
2149 | | > #4,-0.16847,-0.98434,-0.051916,317.82,-0.77209,0.099036,0.62775,149.66,-0.61277,0.14584,-0.77668,323.39 |
2150 | | |
2151 | | > fitmap #4 inMap #1 |
2152 | | |
2153 | | Fit map |
2154 | | W20_J3985_004_volume_map_sharp_csparc_refine_of_relion_job121_bprime_mask.mrc |
2155 | | in map W20_J3623_consensus_structure_sharp.mrc using 138119 points |
2156 | | correlation = 0.9535, correlation about mean = 0.7475, overlap = 3.27e+04 |
2157 | | steps = 128, shift = 2.65, angle = 13.7 degrees |
2158 | | |
2159 | | Position of |
2160 | | W20_J3985_004_volume_map_sharp_csparc_refine_of_relion_job121_bprime_mask.mrc |
2161 | | (#4) relative to W20_J3623_consensus_structure_sharp.mrc (#1) coordinates: |
2162 | | Matrix rotation and translation |
2163 | | -0.34033481 -0.91878664 -0.20000833 354.04060287 |
2164 | | -0.77649015 0.15464973 0.61085719 146.12650484 |
2165 | | -0.53031619 0.36320047 -0.76606146 277.48080009 |
2166 | | Axis -0.56714143 0.75641504 0.32586328 |
2167 | | Axis point 252.06555110 0.00000000 81.39782971 |
2168 | | Rotation angle (degrees) 167.38858263 |
2169 | | Shift along axis 0.16199507 |
2170 | | |
2171 | | |
2172 | | > hide #!4 models |
2173 | | |
2174 | | > select subtract #4 |
2175 | | |
2176 | | Nothing selected |
2177 | | |
2178 | | > show #!5 models |
2179 | | |
2180 | | > show #!6 models |
2181 | | |
2182 | | > show #!7 models |
2183 | | |
2184 | | > show #!8 models |
2185 | | |
2186 | | > show #!9 models |
2187 | | |
2188 | | > hide #!9 models |
2189 | | |
2190 | | > select add #8 |
2191 | | |
2192 | | 2 models selected |
2193 | | |
2194 | | > select add #7 |
2195 | | |
2196 | | 4 models selected |
2197 | | |
2198 | | > select add #6 |
2199 | | |
2200 | | 6 models selected |
2201 | | |
2202 | | > select add #5 |
2203 | | |
2204 | | 8 models selected |
2205 | | |
2206 | | > view matrix models |
2207 | | > #5,1,0,0,10.109,0,1,0,2.5885,0,0,1,20.808,#6,1,0,0,10.109,0,1,0,2.5885,0,0,1,20.808,#7,1,0,0,10.109,0,1,0,2.5885,0,0,1,20.808,#8,1,0,0,10.109,0,1,0,2.5885,0,0,1,20.808 |
2208 | | |
2209 | | > ui mousemode right "rotate selected models" |
2210 | | |
2211 | | > view matrix models |
2212 | | > #5,-0.27105,-0.96135,0.048324,315.79,-0.78137,0.24907,0.5722,138.42,-0.56213,0.11734,-0.81869,331.41,#6,-0.27105,-0.96135,0.048324,315.79,-0.78137,0.24907,0.5722,138.42,-0.56213,0.11734,-0.81869,331.41,#7,-0.27105,-0.96135,0.048324,315.79,-0.78137,0.24907,0.5722,138.42,-0.56213,0.11734,-0.81869,331.41,#8,-0.27105,-0.96135,0.048324,315.79,-0.78137,0.24907,0.5722,138.42,-0.56213,0.11734,-0.81869,331.41 |
2213 | | |
2214 | | > fitmap #5 inMap #1 |
2215 | | |
2216 | | Fit map |
2217 | | W20_J3986_004_volume_map_csparc_refine_of_relion_job122_bprime_mask.mrc in map |
2218 | | W20_J3623_consensus_structure_sharp.mrc using 138161 points |
2219 | | correlation = 0.915, correlation about mean = 0.6261, overlap = 2.286e+04 |
2220 | | steps = 144, shift = 6.68, angle = 15.4 degrees |
2221 | | |
2222 | | Position of |
2223 | | W20_J3986_004_volume_map_csparc_refine_of_relion_job122_bprime_mask.mrc (#5) |
2224 | | relative to W20_J3623_consensus_structure_sharp.mrc (#1) coordinates: |
2225 | | Matrix rotation and translation |
2226 | | -0.33486423 -0.92026440 -0.20243362 353.69981178 |
2227 | | -0.77926293 0.14969047 0.60855734 147.51639631 |
2228 | | -0.52973128 0.36153311 -0.76725393 277.76199824 |
2229 | | Axis -0.56967931 0.75480327 0.32517303 |
2230 | | Axis point 252.70022071 0.00000000 81.39359448 |
2231 | | Rotation angle (degrees) 167.47826779 |
2232 | | Shift along axis 0.17110458 |
2233 | | |
2234 | | |
2235 | | > fitmap #6 inMap #1 |
2236 | | |
2237 | | Fit map |
2238 | | W20_J3986_004_volume_map_sharp_csparc_refine_of_relion_job122_bprime_mask.mrc |
2239 | | in map W20_J3623_consensus_structure_sharp.mrc using 138162 points |
2240 | | correlation = 0.9385, correlation about mean = 0.703, overlap = 3.152e+04 |
2241 | | steps = 124, shift = 6.79, angle = 15.3 degrees |
2242 | | |
2243 | | Position of |
2244 | | W20_J3986_004_volume_map_sharp_csparc_refine_of_relion_job122_bprime_mask.mrc |
2245 | | (#6) relative to W20_J3623_consensus_structure_sharp.mrc (#1) coordinates: |
2246 | | Matrix rotation and translation |
2247 | | -0.33341982 -0.92087059 -0.20206083 353.53923788 |
2248 | | -0.78000897 0.14906211 0.60775528 147.84755443 |
2249 | | -0.52954435 0.36024692 -0.76798759 277.99643882 |
2250 | | Axis -0.57030812 0.75458667 0.32457296 |
2251 | | Axis point 252.86314379 0.00000000 81.47103793 |
2252 | | Rotation angle (degrees) 167.46738658 |
2253 | | Shift along axis 0.16762333 |
2254 | | |
2255 | | |
2256 | | > fitmap #7 inMap #1 |
2257 | | |
2258 | | Fit map |
2259 | | W20_J3987_004_volume_map_csparc_refine_of_relion_job125_bprime_mask.mrc in map |
2260 | | W20_J3623_consensus_structure_sharp.mrc using 138206 points |
2261 | | correlation = 0.9279, correlation about mean = 0.6497, overlap = 2.411e+04 |
2262 | | steps = 136, shift = 6.69, angle = 15.3 degrees |
2263 | | |
2264 | | Position of |
2265 | | W20_J3987_004_volume_map_csparc_refine_of_relion_job125_bprime_mask.mrc (#7) |
2266 | | relative to W20_J3623_consensus_structure_sharp.mrc (#1) coordinates: |
2267 | | Matrix rotation and translation |
2268 | | -0.34035723 -0.91890040 -0.19944673 353.88927693 |
2269 | | -0.77660263 0.15512153 0.61059451 146.07614815 |
2270 | | -0.53013707 0.36271111 -0.76641721 277.57142341 |
2271 | | Axis -0.56711725 0.75656617 0.32555437 |
2272 | | Axis point 251.97967916 0.00000000 81.48001825 |
2273 | | Rotation angle (degrees) 167.37630205 |
2274 | | Shift along axis 0.18414629 |
2275 | | |
2276 | | |
2277 | | > fitmap #8 inMap #1 |
2278 | | |
2279 | | Fit map |
2280 | | W20_J3987_004_volume_map_sharp_csparc_refine_of_relion_job125_bprime_mask.mrc |
2281 | | in map W20_J3623_consensus_structure_sharp.mrc using 138103 points |
2282 | | correlation = 0.9399, correlation about mean = 0.7087, overlap = 4.09e+04 |
2283 | | steps = 132, shift = 6.54, angle = 15.4 degrees |
2284 | | |
2285 | | Position of |
2286 | | W20_J3987_004_volume_map_sharp_csparc_refine_of_relion_job125_bprime_mask.mrc |
2287 | | (#8) relative to W20_J3623_consensus_structure_sharp.mrc (#1) coordinates: |
2288 | | Matrix rotation and translation |
2289 | | -0.34016956 -0.91889502 -0.19979141 353.89977575 |
2290 | | -0.77635698 0.15454414 0.61105314 146.04451039 |
2291 | | -0.53061710 0.36297113 -0.76596178 277.53661238 |
2292 | | Axis -0.56719105 0.75636832 0.32588538 |
2293 | | Axis point 252.01132593 0.00000000 81.40408707 |
2294 | | Rotation angle (degrees) 167.36769172 |
2295 | | Shift along axis 0.17978067 |
2296 | | |
2297 | | |
2298 | | > select subtract #8 |
2299 | | |
2300 | | 6 models selected |
2301 | | |
2302 | | > select subtract #7 |
2303 | | |
2304 | | 4 models selected |
2305 | | |
2306 | | > select subtract #6 |
2307 | | |
2308 | | 2 models selected |
2309 | | |
2310 | | > select subtract #5 |
2311 | | |
2312 | | Nothing selected |
2313 | | |
2314 | | > hide #!8 models |
2315 | | |
2316 | | > hide #!7 models |
2317 | | |
2318 | | > hide #!6 models |
2319 | | |
2320 | | > hide #!5 models |
2321 | | |
2322 | | > show #!9 models |
2323 | | |
2324 | | > show #!10 models |
2325 | | |
2326 | | > show #!11 models |
2327 | | |
2328 | | > hide #!11 models |
2329 | | |
2330 | | > select add #10 |
2331 | | |
2332 | | 2 models selected |
2333 | | |
2334 | | > select add #9 |
2335 | | |
2336 | | 4 models selected |
2337 | | |
2338 | | > view matrix models |
2339 | | > #9,-0.20458,-0.9646,-0.16641,334.03,-0.77373,0.055221,0.6311,156.33,-0.59957,0.25787,-0.75764,298.39,#10,-0.20458,-0.9646,-0.16641,334.03,-0.77373,0.055221,0.6311,156.33,-0.59957,0.25787,-0.75764,298.39 |
2340 | | |
2341 | | > ui mousemode right "translate selected models" |
2342 | | |
2343 | | > view matrix models |
2344 | | > #9,-0.20458,-0.9646,-0.16641,335.57,-0.77373,0.055221,0.6311,156.39,-0.59957,0.25787,-0.75764,301.3,#10,-0.20458,-0.9646,-0.16641,335.57,-0.77373,0.055221,0.6311,156.39,-0.59957,0.25787,-0.75764,301.3 |
2345 | | |
2346 | | > fitmap #9 inMap #1 |
2347 | | |
2348 | | Fit map |
2349 | | W20_J3988_004_volume_map_csparc_refine_of_relion_job126_gammazeta_mask.mrc in |
2350 | | map W20_J3623_consensus_structure_sharp.mrc using 138204 points |
2351 | | correlation = 0.9071, correlation about mean = 0.5649, overlap = 2.482e+04 |
2352 | | steps = 92, shift = 0.476, angle = 8.65 degrees |
2353 | | |
2354 | | Position of |
2355 | | W20_J3988_004_volume_map_csparc_refine_of_relion_job126_gammazeta_mask.mrc |
2356 | | (#9) relative to W20_J3623_consensus_structure_sharp.mrc (#1) coordinates: |
2357 | | Matrix rotation and translation |
2358 | | -0.33561246 -0.92104503 -0.19758628 353.34320386 |
2359 | | -0.77965973 0.15387748 0.60700283 147.26083252 |
2360 | | -0.52867286 0.35776778 -0.76974491 278.24593808 |
2361 | | Axis -0.56920453 0.75613754 0.32289661 |
2362 | | Axis point 252.48560152 0.00000000 81.78647209 |
2363 | | Rotation angle (degrees) 167.35364255 |
2364 | | Shift along axis 0.06955954 |
2365 | | |
2366 | | |
2367 | | > fitmap #10 inMap #1 |
2368 | | |
2369 | | Fit map |
2370 | | W20_J3988_004_volume_map_sharp_csparc_refine_of_relion_job126_gammazeta_mask.mrc |
2371 | | in map W20_J3623_consensus_structure_sharp.mrc using 138104 points |
2372 | | correlation = 0.9176, correlation about mean = 0.6072, overlap = 3.365e+04 |
2373 | | steps = 96, shift = 0.462, angle = 8.63 degrees |
2374 | | |
2375 | | Position of |
2376 | | W20_J3988_004_volume_map_sharp_csparc_refine_of_relion_job126_gammazeta_mask.mrc |
2377 | | (#10) relative to W20_J3623_consensus_structure_sharp.mrc (#1) coordinates: |
2378 | | Matrix rotation and translation |
2379 | | -0.33524620 -0.92121016 -0.19743815 353.30178908 |
2380 | | -0.77963330 0.15360588 0.60710555 147.28662723 |
2381 | | -0.52894414 0.35745918 -0.76970191 278.32308895 |
2382 | | Axis -0.56934667 0.75603676 0.32288198 |
2383 | | Axis point 252.53350589 0.00000000 81.77924134 |
2384 | | Rotation angle (degrees) 167.33564064 |
2385 | | Shift along axis 0.06841760 |
2386 | | |
2387 | | |
2388 | | > select subtract #10 |
2389 | | |
2390 | | 2 models selected |
2391 | | |
2392 | | > select subtract #9 |
2393 | | |
2394 | | Nothing selected |
2395 | | |
2396 | | > hide #!9 models |
2397 | | |
2398 | | > hide #!10 models |
2399 | | |
2400 | | > show #!11 models |
2401 | | |
2402 | | > show #!12 models |
2403 | | |
2404 | | > volume #11 level 1.414 |
2405 | | |
2406 | | > volume #12 level 1.511 |
2407 | | |
2408 | | > select add #11 |
2409 | | |
2410 | | 2 models selected |
2411 | | |
2412 | | > select add #12 |
2413 | | |
2414 | | 4 models selected |
2415 | | |
2416 | | > ui mousemode right "rotate selected models" |
2417 | | |
2418 | | > view matrix models |
2419 | | > #11,-0.12102,-0.028286,0.99225,32.717,0.99084,0.056881,0.12247,-30.578,-0.059904,0.99798,0.021144,3.463,#12,-0.12102,-0.028286,0.99225,32.717,0.99084,0.056881,0.12247,-30.578,-0.059904,0.99798,0.021144,3.463 |
2420 | | |
2421 | | > view matrix models |
2422 | | > #11,-0.047287,-0.19762,0.97914,47.757,0.3239,0.92424,0.20219,-66.044,-0.94491,0.3267,0.020305,232.25,#12,-0.047287,-0.19762,0.97914,47.757,0.3239,0.92424,0.20219,-66.044,-0.94491,0.3267,0.020305,232.25 |
2423 | | |
2424 | | > view matrix models |
2425 | | > #11,-0.88887,-0.45801,0.011781,346.86,-0.23966,0.48671,0.84005,-8.5049,-0.39048,0.74387,-0.54239,168.45,#12,-0.88887,-0.45801,0.011781,346.86,-0.23966,0.48671,0.84005,-8.5049,-0.39048,0.74387,-0.54239,168.45 |
2426 | | |
2427 | | > view matrix models |
2428 | | > #11,-0.19168,-0.96873,-0.15754,339.31,-0.75924,0.044642,0.64928,159.39,-0.62194,0.24407,-0.74406,302.98,#12,-0.19168,-0.96873,-0.15754,339.31,-0.75924,0.044642,0.64928,159.39,-0.62194,0.24407,-0.74406,302.98 |
2429 | | |
2430 | | > fitmap #11 inMap #1 |
2431 | | |
2432 | | Fit map |
2433 | | W20_J3989_004_volume_map_csparc_refine_of_relion_job127_gammazeta_mask.mrc in |
2434 | | map W20_J3623_consensus_structure_sharp.mrc using 42734 points |
2435 | | correlation = 0.9154, correlation about mean = 0.4456, overlap = 5.735e+04 |
2436 | | steps = 104, shift = 9.56, angle = 10.3 degrees |
2437 | | |
2438 | | Position of |
2439 | | W20_J3989_004_volume_map_csparc_refine_of_relion_job127_gammazeta_mask.mrc |
2440 | | (#11) relative to W20_J3623_consensus_structure_sharp.mrc (#1) coordinates: |
2441 | | Matrix rotation and translation |
2442 | | -0.34146109 -0.91804101 -0.20150685 354.32329233 |
2443 | | -0.77726306 0.15526879 0.60971610 146.26157419 |
2444 | | -0.52845666 0.36481815 -0.76657763 277.10408311 |
2445 | | Axis -0.56679376 0.75669526 0.32581761 |
2446 | | Axis point 251.95105256 0.00000000 81.46580342 |
2447 | | Rotation angle (degrees) 167.52357003 |
2448 | | Shift along axis 0.13259978 |
2449 | | |
2450 | | |
2451 | | > fitmap #12 inMap #1 |
2452 | | |
2453 | | Fit map |
2454 | | W20_J3989_004_volume_map_sharp_csparc_refine_of_relion_job127_gammazeta_mask.mrc |
2455 | | in map W20_J3623_consensus_structure_sharp.mrc using 37926 points |
2456 | | correlation = 0.9185, correlation about mean = 0.4316, overlap = 5.437e+04 |
2457 | | steps = 120, shift = 9.52, angle = 10.3 degrees |
2458 | | |
2459 | | Position of |
2460 | | W20_J3989_004_volume_map_sharp_csparc_refine_of_relion_job127_gammazeta_mask.mrc |
2461 | | (#12) relative to W20_J3623_consensus_structure_sharp.mrc (#1) coordinates: |
2462 | | Matrix rotation and translation |
2463 | | -0.34132328 -0.91808135 -0.20155652 354.32310537 |
2464 | | -0.77719944 0.15505915 0.60985055 146.27901701 |
2465 | | -0.52863923 0.36480581 -0.76645762 277.14013363 |
2466 | | Axis -0.56684797 0.75662172 0.32589408 |
2467 | | Axis point 251.98254766 0.00000000 81.45327260 |
2468 | | Rotation angle (degrees) 167.51718440 |
2469 | | Shift along axis 0.14887713 |
2470 | | |
2471 | | |
2472 | | > select subtract #12 |
2473 | | |
2474 | | 2 models selected |
2475 | | |
2476 | | > select subtract #11 |
2477 | | |
2478 | | Nothing selected |
2479 | | |
2480 | | > hide #!1 models |
2481 | | |
2482 | | > volume #11 level 1.414 |
2483 | | |
2484 | | > hide #!11 models |
2485 | | |
2486 | | > hide #!12 models |
2487 | | |
2488 | | > show #!13 models |
2489 | | |
2490 | | > show #!1 models |
2491 | | |
2492 | | > show #!14 models |
2493 | | |
2494 | | > fitmap #13 inMap #1 |
2495 | | |
2496 | | Fit map W20_J4021_005_volume_map_bprime_local.mrc in map |
2497 | | W20_J3623_consensus_structure_sharp.mrc using 138166 points |
2498 | | correlation = 0.8362, correlation about mean = 0.2198, overlap = 1.935e+04 |
2499 | | steps = 64, shift = 0.521, angle = 0.419 degrees |
2500 | | |
2501 | | Position of W20_J4021_005_volume_map_bprime_local.mrc (#13) relative to |
2502 | | W20_J3623_consensus_structure_sharp.mrc (#1) coordinates: |
2503 | | Matrix rotation and translation |
2504 | | 0.99998250 0.00590533 0.00034832 -0.90323251 |
2505 | | -0.00590377 0.99997332 -0.00430079 1.11548441 |
2506 | | -0.00037371 0.00429865 0.99999069 -0.31823043 |
2507 | | Axis 0.58794667 0.04936530 -0.80739196 |
2508 | | Axis point 190.18082923 131.70665579 0.00000000 |
2509 | | Rotation angle (degrees) 0.41901428 |
2510 | | Shift along axis -0.21904963 |
2511 | | |
2512 | | |
2513 | | > fitmap #14 inMap #1 |
2514 | | |
2515 | | Fit map W20_J4021_005_volume_map_sharp_bprime_local.mrc in map |
2516 | | W20_J3623_consensus_structure_sharp.mrc using 137893 points |
2517 | | correlation = 0.8085, correlation about mean = 0.2734, overlap = 2.896e+04 |
2518 | | steps = 48, shift = 0.598, angle = 0.487 degrees |
2519 | | |
2520 | | Position of W20_J4021_005_volume_map_sharp_bprime_local.mrc (#14) relative to |
2521 | | W20_J3623_consensus_structure_sharp.mrc (#1) coordinates: |
2522 | | Matrix rotation and translation |
2523 | | 0.99997911 0.00638470 0.00100265 -1.04735728 |
2524 | | -0.00637907 0.99996444 -0.00551663 1.34954856 |
2525 | | -0.00103783 0.00551011 0.99998428 -0.33308547 |
2526 | | Axis 0.64900636 0.12009752 -0.75124386 |
2527 | | Axis point 215.82672874 137.56284372 -0.00000000 |
2528 | | Rotation angle (degrees) 0.48673885 |
2529 | | Shift along axis -0.26743568 |
2530 | | |
2531 | | |
2532 | | > hide #!14 models |
2533 | | |
2534 | | > hide #!13 models |
2535 | | |
2536 | | > show #!15 models |
2537 | | |
2538 | | > show #!16 models |
2539 | | |
2540 | | > fitmap #15 inMap #1 |
2541 | | |
2542 | | Fit map W20_J4022_005_volume_map_bprime_local.mrc in map |
2543 | | W20_J3623_consensus_structure_sharp.mrc using 138200 points |
2544 | | correlation = 0.8381, correlation about mean = 0.222, overlap = 1.906e+04 |
2545 | | steps = 40, shift = 0.303, angle = 0.34 degrees |
2546 | | |
2547 | | Position of W20_J4022_005_volume_map_bprime_local.mrc (#15) relative to |
2548 | | W20_J3623_consensus_structure_sharp.mrc (#1) coordinates: |
2549 | | Matrix rotation and translation |
2550 | | 0.99998447 0.00240641 0.00502780 -1.28358869 |
2551 | | -0.00241668 0.99999501 0.00203622 -0.22148190 |
2552 | | -0.00502288 -0.00204834 0.99998529 1.05482120 |
2553 | | Axis -0.34402829 0.84653419 -0.40623194 |
2554 | | Axis point 195.11366625 0.00000000 267.83446555 |
2555 | | Rotation angle (degrees) 0.34013109 |
2556 | | Shift along axis -0.17440325 |
2557 | | |
2558 | | |
2559 | | > fitmap #16 inMap #1 |
2560 | | |
2561 | | Fit map W20_J4022_005_volume_map_sharp_bprime_local.mrc in map |
2562 | | W20_J3623_consensus_structure_sharp.mrc using 138084 points |
2563 | | correlation = 0.8046, correlation about mean = 0.274, overlap = 2.955e+04 |
2564 | | steps = 48, shift = 0.324, angle = 0.293 degrees |
2565 | | |
2566 | | Position of W20_J4022_005_volume_map_sharp_bprime_local.mrc (#16) relative to |
2567 | | W20_J3623_consensus_structure_sharp.mrc (#1) coordinates: |
2568 | | Matrix rotation and translation |
2569 | | 0.99999153 0.00141184 0.00386537 -0.96250876 |
2570 | | -0.00142356 0.99999440 0.00302979 -0.53091994 |
2571 | | -0.00386107 -0.00303526 0.99998794 0.99298679 |
2572 | | Axis -0.59323978 0.75574456 -0.27733863 |
2573 | | Axis point 248.76134938 0.00000000 265.76556629 |
2574 | | Rotation angle (degrees) 0.29288616 |
2575 | | Shift along axis -0.10563498 |
2576 | | |
2577 | | |
2578 | | > hide #!16 models |
2579 | | |
2580 | | > hide #!15 models |
2581 | | |
2582 | | > show #!17 models |
2583 | | |
2584 | | > fitmap #17 inMap #1 |
2585 | | |
2586 | | Fit map W20_J4023_003_volume_map_bprime_local.mrc in map |
2587 | | W20_J3623_consensus_structure_sharp.mrc using 138098 points |
2588 | | correlation = 0.8373, correlation about mean = 0.2151, overlap = 1.874e+04 |
2589 | | steps = 68, shift = 0.363, angle = 0.333 degrees |
2590 | | |
2591 | | Position of W20_J4023_003_volume_map_bprime_local.mrc (#17) relative to |
2592 | | W20_J3623_consensus_structure_sharp.mrc (#1) coordinates: |
2593 | | Matrix rotation and translation |
2594 | | 0.99998345 0.00557936 0.00140325 -1.18658067 |
2595 | | -0.00557823 0.99998411 -0.00081217 0.60425107 |
2596 | | -0.00140776 0.00080432 0.99999869 0.20586820 |
2597 | | Axis 0.13912185 0.24192785 -0.96026873 |
2598 | | Axis point 117.12498270 207.62002695 0.00000000 |
2599 | | Rotation angle (degrees) 0.33286852 |
2600 | | Shift along axis -0.21658293 |
2601 | | |
2602 | | |
2603 | | > show #!18 models |
2604 | | |
2605 | | > fitmap #18 inMap #1 |
2606 | | |
2607 | | Fit map W20_J4023_003_volume_map_sharp_bprime_local.mrc in map |
2608 | | W20_J3623_consensus_structure_sharp.mrc using 138171 points |
2609 | | correlation = 0.7947, correlation about mean = 0.312, overlap = 3.526e+04 |
2610 | | steps = 68, shift = 0.461, angle = 0.264 degrees |
2611 | | |
2612 | | Position of W20_J4023_003_volume_map_sharp_bprime_local.mrc (#18) relative to |
2613 | | W20_J3623_consensus_structure_sharp.mrc (#1) coordinates: |
2614 | | Matrix rotation and translation |
2615 | | 0.99999048 0.00393326 0.00188760 -1.06407059 |
2616 | | -0.00393046 0.99999117 -0.00148458 0.41525484 |
2617 | | -0.00189342 0.00147715 0.99999712 0.25585356 |
2618 | | Axis 0.32142171 0.41033635 -0.85341207 |
2619 | | Axis point 145.82615217 239.01637261 0.00000000 |
2620 | | Rotation angle (degrees) 0.26397543 |
2621 | | Shift along axis -0.38996975 |
2622 | | |
2623 | | |
2624 | | > hide #!18 models |
2625 | | |
2626 | | > hide #!17 models |
2627 | | |
2628 | | > show #!19 models |
2629 | | |
2630 | | > fitmap #19 inMap #1 |
2631 | | |
2632 | | Fit map W20_J4024_005_volume_map_bprime_local.mrc in map |
2633 | | W20_J3623_consensus_structure_sharp.mrc using 138193 points |
2634 | | correlation = 0.8392, correlation about mean = 0.2773, overlap = 1.921e+04 |
2635 | | steps = 76, shift = 0.289, angle = 0.259 degrees |
2636 | | |
2637 | | Position of W20_J4024_005_volume_map_bprime_local.mrc (#19) relative to |
2638 | | W20_J3623_consensus_structure_sharp.mrc (#1) coordinates: |
2639 | | Matrix rotation and translation |
2640 | | 0.99999040 0.00368324 0.00237314 -0.77269801 |
2641 | | -0.00368586 0.99999260 0.00110036 0.16957827 |
2642 | | -0.00236907 -0.00110910 0.99999658 0.39066327 |
2643 | | Axis -0.24448082 0.52473318 -0.81540433 |
2644 | | Axis point 51.36886426 212.61995395 0.00000000 |
2645 | | Rotation angle (degrees) 0.25890214 |
2646 | | Shift along axis -0.04065533 |
2647 | | |
2648 | | |
2649 | | > fitmap #20 inMap #1 |
2650 | | |
2651 | | Fit map W20_J4024_005_volume_map_sharp_bprime_local.mrc in map |
2652 | | W20_J3623_consensus_structure_sharp.mrc using 138123 points |
2653 | | correlation = 0.8039, correlation about mean = 0.2975, overlap = 2.959e+04 |
2654 | | steps = 48, shift = 0.294, angle = 0.249 degrees |
2655 | | |
2656 | | Position of W20_J4024_005_volume_map_sharp_bprime_local.mrc (#20) relative to |
2657 | | W20_J3623_consensus_structure_sharp.mrc (#1) coordinates: |
2658 | | Matrix rotation and translation |
2659 | | 0.99999177 0.00343445 0.00216100 -0.68440650 |
2660 | | -0.00343784 0.99999286 0.00156920 0.06668376 |
2661 | | -0.00215559 -0.00157662 0.99999643 0.45265088 |
2662 | | Axis -0.36142806 0.49593828 -0.78956633 |
2663 | | Axis point 30.06869516 207.44842694 0.00000000 |
2664 | | Rotation angle (degrees) 0.24934854 |
2665 | | Shift along axis -0.07696315 |
2666 | | |
2667 | | |
2668 | | > hide #!19 models |
2669 | | |
2670 | | > show #!20 models |
2671 | | |
2672 | | > hide #!20 models |
2673 | | |
2674 | | > show #!21 models |
2675 | | |
2676 | | > fitmap #21 inMap #1 |
2677 | | |
2678 | | Fit map W20_J4025_004_volume_map_bprime_local.mrc in map |
2679 | | W20_J3623_consensus_structure_sharp.mrc using 138084 points |
2680 | | correlation = 0.8289, correlation about mean = 0.1891, overlap = 1.832e+04 |
2681 | | steps = 48, shift = 0.728, angle = 0.413 degrees |
2682 | | |
2683 | | Position of W20_J4025_004_volume_map_bprime_local.mrc (#21) relative to |
2684 | | W20_J3623_consensus_structure_sharp.mrc (#1) coordinates: |
2685 | | Matrix rotation and translation |
2686 | | 0.99998460 0.00544119 0.00108928 -0.83012202 |
2687 | | -0.00543611 0.99997458 -0.00461454 1.00696868 |
2688 | | -0.00111436 0.00460855 0.99998876 -0.07337997 |
2689 | | Axis 0.63914215 0.15270809 -0.75377553 |
2690 | | Axis point 193.72853355 115.34838902 0.00000000 |
2691 | | Rotation angle (degrees) 0.41340458 |
2692 | | Shift along axis -0.32148169 |
2693 | | |
2694 | | |
2695 | | > fitmap #22 inMap #1 |
2696 | | |
2697 | | Fit map W20_J4025_004_volume_map_sharp_bprime_local.mrc in map |
2698 | | W20_J3623_consensus_structure_sharp.mrc using 137654 points |
2699 | | correlation = 0.7511, correlation about mean = 0.2821, overlap = 3.85e+04 |
2700 | | steps = 52, shift = 0.819, angle = 0.498 degrees |
2701 | | |
2702 | | Position of W20_J4025_004_volume_map_sharp_bprime_local.mrc (#22) relative to |
2703 | | W20_J3623_consensus_structure_sharp.mrc (#1) coordinates: |
2704 | | Matrix rotation and translation |
2705 | | 0.99998155 0.00559478 0.00236633 -1.03081576 |
2706 | | -0.00557996 0.99996507 -0.00622345 1.34930492 |
2707 | | -0.00240107 0.00621013 0.99997783 -0.00730733 |
2708 | | Axis 0.71523902 0.27424352 -0.64282473 |
2709 | | Axis point 0.00000000 39.53456477 232.56399021 |
2710 | | Rotation angle (degrees) 0.49801542 |
2711 | | Shift along axis -0.36254420 |
2712 | | |
2713 | | |
2714 | | > show #!22 models |
2715 | | |
2716 | | > hide #!22 models |
2717 | | |
2718 | | > hide #!21 models |
2719 | | |
2720 | | > show #!23 models |
2721 | | |
2722 | | > show #!24 models |
2723 | | |
2724 | | > fitmap #23 inMap #1 |
2725 | | |
2726 | | Fit map W20_J4026_004_volume_map_gammazeta_local.mrc in map |
2727 | | W20_J3623_consensus_structure_sharp.mrc using 138172 points |
2728 | | correlation = 0.8607, correlation about mean = 0.3259, overlap = 2.201e+04 |
2729 | | steps = 52, shift = 0.541, angle = 0.494 degrees |
2730 | | |
2731 | | Position of W20_J4026_004_volume_map_gammazeta_local.mrc (#23) relative to |
2732 | | W20_J3623_consensus_structure_sharp.mrc (#1) coordinates: |
2733 | | Matrix rotation and translation |
2734 | | 0.99997866 -0.00017168 -0.00653102 0.57575571 |
2735 | | 0.00020836 0.99998421 0.00561565 -0.37594588 |
2736 | | 0.00652995 -0.00561689 0.99996290 0.02009032 |
2737 | | Axis -0.65188430 -0.75799762 0.02205601 |
2738 | | Axis point -2.93047519 0.00000000 79.19172412 |
2739 | | Rotation angle (degrees) 0.49363480 |
2740 | | Shift along axis -0.08991691 |
2741 | | |
2742 | | |
2743 | | > fitmap #24 inMap #1 |
2744 | | |
2745 | | Fit map W20_J4026_004_volume_map_sharp_gammazeta_local.mrc in map |
2746 | | W20_J3623_consensus_structure_sharp.mrc using 138033 points |
2747 | | correlation = 0.853, correlation about mean = 0.3795, overlap = 2.779e+04 |
2748 | | steps = 68, shift = 0.477, angle = 0.571 degrees |
2749 | | |
2750 | | Position of W20_J4026_004_volume_map_sharp_gammazeta_local.mrc (#24) relative |
2751 | | to W20_J3623_consensus_structure_sharp.mrc (#1) coordinates: |
2752 | | Matrix rotation and translation |
2753 | | 0.99996930 -0.00126227 -0.00773336 0.96231167 |
2754 | | 0.00130980 0.99998026 0.00614469 -0.62594677 |
2755 | | 0.00772545 -0.00615463 0.99995122 -0.10724743 |
2756 | | Axis -0.61739139 -0.77598860 0.12911066 |
2757 | | Axis point 12.56321001 0.00000000 114.62740380 |
2758 | | Rotation angle (degrees) 0.57071652 |
2759 | | Shift along axis -0.12224217 |
2760 | | |
2761 | | |
2762 | | > hide #!24 models |
2763 | | |
2764 | | > hide #!23 models |
2765 | | |
2766 | | > show #!25 models |
2767 | | |
2768 | | > show #!26 models |
2769 | | |
2770 | | > fitmap #25 inMap #1 |
2771 | | |
2772 | | Fit map W20_J4027_005_volume_map_gammazeta_local.mrc in map |
2773 | | W20_J3623_consensus_structure_sharp.mrc using 138160 points |
2774 | | correlation = 0.8845, correlation about mean = 0.4248, overlap = 2.208e+04 |
2775 | | steps = 48, shift = 0.937, angle = 0.528 degrees |
2776 | | |
2777 | | Position of W20_J4027_005_volume_map_gammazeta_local.mrc (#25) relative to |
2778 | | W20_J3623_consensus_structure_sharp.mrc (#1) coordinates: |
2779 | | Matrix rotation and translation |
2780 | | 0.99995803 -0.00131908 0.00906657 -1.56861951 |
2781 | | 0.00131039 0.99999868 0.00096483 0.05951128 |
2782 | | -0.00906783 -0.00095291 0.99995843 2.12791102 |
2783 | | Axis -0.10408825 0.98427482 0.14271899 |
2784 | | Axis point 225.62409153 0.00000000 168.02231673 |
2785 | | Rotation angle (degrees) 0.52781987 |
2786 | | Shift along axis 0.52554363 |
2787 | | |
2788 | | |
2789 | | > fitmap #26 inMap #1 |
2790 | | |
2791 | | Fit map W20_J4027_005_volume_map_sharp_gammazeta_local.mrc in map |
2792 | | W20_J3623_consensus_structure_sharp.mrc using 138211 points |
2793 | | correlation = 0.8774, correlation about mean = 0.4648, overlap = 2.896e+04 |
2794 | | steps = 48, shift = 0.956, angle = 0.621 degrees |
2795 | | |
2796 | | Position of W20_J4027_005_volume_map_sharp_gammazeta_local.mrc (#26) relative |
2797 | | to W20_J3623_consensus_structure_sharp.mrc (#1) coordinates: |
2798 | | Matrix rotation and translation |
2799 | | 0.99994135 -0.00098967 0.01078522 -1.85651387 |
2800 | | 0.00098601 0.99999945 0.00034465 0.18754839 |
2801 | | -0.01078555 -0.00033399 0.99994178 2.30481893 |
2802 | | Axis -0.03131444 0.99534338 0.09116443 |
2803 | | Axis point 208.92157622 0.00000000 171.95034602 |
2804 | | Rotation angle (degrees) 0.62086019 |
2805 | | Shift along axis 0.45492826 |
2806 | | |
2807 | | |
2808 | | > hide #!26 models |
2809 | | |
2810 | | > hide #!25 models |
2811 | | |
2812 | | > show #!27 models |
2813 | | |
2814 | | > fitmap #27 inMap #1 |
2815 | | |
2816 | | Fit map W20_J4028_004_volume_map_gammazeta_local.mrc in map |
2817 | | W20_J3623_consensus_structure_sharp.mrc using 138218 points |
2818 | | correlation = 0.8752, correlation about mean = 0.4062, overlap = 2.23e+04 |
2819 | | steps = 44, shift = 0.478, angle = 0.403 degrees |
2820 | | |
2821 | | Position of W20_J4028_004_volume_map_gammazeta_local.mrc (#27) relative to |
2822 | | W20_J3623_consensus_structure_sharp.mrc (#1) coordinates: |
2823 | | Matrix rotation and translation |
2824 | | 0.99997775 0.00042654 -0.00665697 0.77827945 |
2825 | | -0.00041158 0.99999739 0.00224763 0.14049133 |
2826 | | 0.00665791 -0.00224484 0.99997532 -0.37918036 |
2827 | | Axis -0.31912872 -0.94583944 -0.05953667 |
2828 | | Axis point 60.52816960 0.00000000 99.51494449 |
2829 | | Rotation angle (degrees) 0.40328886 |
2830 | | Shift along axis -0.35867843 |
2831 | | |
2832 | | |
2833 | | > fitmap #28 inMap #1 |
2834 | | |
2835 | | Fit map W20_J4028_004_volume_map_sharp_gammazeta_local.mrc in map |
2836 | | W20_J3623_consensus_structure_sharp.mrc using 137995 points |
2837 | | correlation = 0.8679, correlation about mean = 0.4447, overlap = 2.897e+04 |
2838 | | steps = 48, shift = 0.432, angle = 0.487 degrees |
2839 | | |
2840 | | Position of W20_J4028_004_volume_map_sharp_gammazeta_local.mrc (#28) relative |
2841 | | to W20_J3623_consensus_structure_sharp.mrc (#1) coordinates: |
2842 | | Matrix rotation and translation |
2843 | | 0.99996809 -0.00001305 -0.00798826 1.06130917 |
2844 | | 0.00003628 0.99999577 0.00290797 -0.02300922 |
2845 | | 0.00798819 -0.00290817 0.99996387 -0.53329988 |
2846 | | Axis -0.34208068 -0.93966610 0.00290175 |
2847 | | Axis point 67.16980128 0.00000000 117.90291440 |
2848 | | Rotation angle (degrees) 0.48708480 |
2849 | | Shift along axis -0.34297988 |
2850 | | |
2851 | | |
2852 | | > show #!28 models |
2853 | | |
2854 | | > hide #!28 models |
2855 | | |
2856 | | > show #!28 models |
2857 | | |
2858 | | > hide #!28 models |
2859 | | |
2860 | | > hide #!27 models |
2861 | | |
2862 | | > save "/Users/becca/Desktop/Postdoc/COPI notebooks and |
2863 | | > resources/Processing/W20_MM_cyto_COPI/20250701_W20_COPI_cyto_alignments.cxs" |
2864 | | |
2865 | | > open "/Users/becca/Desktop/Postdoc/COPI notebooks and |
2866 | | > resources/Processing/W20_MM_cyto_COPI/relionMB/W20_J140_run_body001.mrc" |
2867 | | > "/Users/becca/Desktop/Postdoc/COPI notebooks and |
2868 | | > resources/Processing/W20_MM_cyto_COPI/relionMB/W20_J140_run_body002.mrc" |
2869 | | > "/Users/becca/Desktop/Postdoc/COPI notebooks and |
2870 | | > resources/Processing/W20_MM_cyto_COPI/relionMB/W20_J140_run_body003.mrc" |
2871 | | > "/Users/becca/Desktop/Postdoc/COPI notebooks and |
2872 | | > resources/Processing/W20_MM_cyto_COPI/relionMB/W20_J140_run_body004.mrc" |
2873 | | |
2874 | | Opened W20_J140_run_body001.mrc as #29.1, grid size 240,240,240, pixel 1.2, |
2875 | | shown at level 0.00608, step 1, values float32 |
2876 | | Opened W20_J140_run_body002.mrc as #29.2, grid size 240,240,240, pixel 1.2, |
2877 | | shown at level 0.00134, step 1, values float32 |
2878 | | Opened W20_J140_run_body003.mrc as #29.3, grid size 240,240,240, pixel 1.2, |
2879 | | shown at level 0.000955, step 1, values float32 |
2880 | | Opened W20_J140_run_body004.mrc as #29.4, grid size 240,240,240, pixel 1.2, |
2881 | | shown at level 0.000493, step 1, values float32 |
2882 | | |
2883 | | > select add #29 |
2884 | | |
2885 | | 9 models selected |
2886 | | |
2887 | | > view matrix models |
2888 | | > #29,-0.47366,-0.87946,-0.046912,333.91,-0.83829,0.43388,0.33018,153.37,-0.27003,0.19572,-0.94275,297.34 |
2889 | | |
2890 | | > ui mousemode right "translate selected models" |
2891 | | |
2892 | | > view matrix models |
2893 | | > #29,-0.47366,-0.87946,-0.046912,340.41,-0.83829,0.43388,0.33018,139.03,-0.27003,0.19572,-0.94275,298.38 |
2894 | | |
2895 | | > fitmap #29 inMap #1 |
2896 | | |
2897 | | Multiple maps for #29 |
2898 | | |
2899 | | > fitmap #29.1 inMap #1 |
2900 | | |
2901 | | Fit map W20_J140_run_body001.mrc in map |
2902 | | W20_J3623_consensus_structure_sharp.mrc using 138225 points |
2903 | | correlation = 0.9233, correlation about mean = 0.7294, overlap = 874.3 |
2904 | | steps = 156, shift = 14.4, angle = 22 degrees |
2905 | | |
2906 | | Position of W20_J140_run_body001.mrc (#29.1) relative to |
2907 | | W20_J3623_consensus_structure_sharp.mrc (#1) coordinates: |
2908 | | Matrix rotation and translation |
2909 | | 0.95200729 0.18684598 -0.24242669 1.82192845 |
2910 | | -0.12799492 0.96249869 0.23919359 -9.97975402 |
2911 | | 0.27802773 -0.19668465 0.94022111 9.66133125 |
2912 | | Axis -0.58247333 -0.69549428 -0.42072857 |
2913 | | Axis point -33.51580829 0.00000000 18.51090823 |
2914 | | Rotation angle (degrees) 21.97250066 |
2915 | | Shift along axis 1.81483897 |
2916 | | |
2917 | | |
2918 | | > fitmap #29.2 inMap #1 |
2919 | | |
2920 | | Fit map W20_J140_run_body002.mrc in map |
2921 | | W20_J3623_consensus_structure_sharp.mrc using 138046 points |
2922 | | correlation = 0.8263, correlation about mean = 0.6371, overlap = 468.8 |
2923 | | steps = 280, shift = 31, angle = 21.8 degrees |
2924 | | |
2925 | | Position of W20_J140_run_body002.mrc (#29.2) relative to |
2926 | | W20_J3623_consensus_structure_sharp.mrc (#1) coordinates: |
2927 | | Matrix rotation and translation |
2928 | | 0.95172436 0.17711878 -0.25069840 4.25540621 |
2929 | | -0.11810903 0.96515525 0.23350719 -10.43311409 |
2930 | | 0.28332138 -0.19262473 0.93948108 8.13777130 |
2931 | | Axis -0.57255681 -0.71751644 -0.39667222 |
2932 | | Axis point -25.70139502 0.00000000 26.08327898 |
2933 | | Rotation angle (degrees) 21.84708246 |
2934 | | Shift along axis 1.82144130 |
2935 | | |
2936 | | |
2937 | | > fitmap #29.3 inMap #1 |
2938 | | |
2939 | | Fit map W20_J140_run_body003.mrc in map |
2940 | | W20_J3623_consensus_structure_sharp.mrc using 137660 points |
2941 | | correlation = 0.5389, correlation about mean = 0.1615, overlap = 221.3 |
2942 | | steps = 180, shift = 25.4, angle = 11.9 degrees |
2943 | | |
2944 | | Position of W20_J140_run_body003.mrc (#29.3) relative to |
2945 | | W20_J3623_consensus_structure_sharp.mrc (#1) coordinates: |
2946 | | Matrix rotation and translation |
2947 | | 0.98378563 0.15518997 -0.08989941 -25.87512376 |
2948 | | -0.14501363 0.98324752 0.11043264 11.64818978 |
2949 | | 0.10553141 -0.09560540 0.98980944 10.88183562 |
2950 | | Axis -0.49859202 -0.47292359 -0.72646353 |
2951 | | Axis point 58.49450968 174.49109696 0.00000000 |
2952 | | Rotation angle (degrees) 11.92434111 |
2953 | | Shift along axis -0.51283023 |
2954 | | |
2955 | | |
2956 | | > fitmap #29.4 inMap #1 |
2957 | | |
2958 | | Fit map W20_J140_run_body004.mrc in map |
2959 | | W20_J3623_consensus_structure_sharp.mrc using 137970 points |
2960 | | correlation = 0.7171, correlation about mean = 0.4553, overlap = 200.6 |
2961 | | steps = 192, shift = 16.7, angle = 21.7 degrees |
2962 | | |
2963 | | Position of W20_J140_run_body004.mrc (#29.4) relative to |
2964 | | W20_J3623_consensus_structure_sharp.mrc (#1) coordinates: |
2965 | | Matrix rotation and translation |
2966 | | 0.95301433 0.19868762 -0.22866331 -0.93326951 |
2967 | | -0.14395018 0.96121397 0.23525740 -8.31020320 |
2968 | | 0.26653710 -0.19128756 0.94465181 8.81821407 |
2969 | | Axis -0.57801628 -0.67105211 -0.46431266 |
2970 | | Axis point -34.90313676 0.00000000 8.20072337 |
2971 | | Rotation angle (degrees) 21.65230186 |
2972 | | Shift along axis 2.02161595 |
2973 | | |
2974 | | |
2975 | | > select subtract #29 |
2976 | | |
2977 | | Nothing selected |
2978 | | |
2979 | | > select add #29 |
2980 | | |
2981 | | 9 models selected |
2982 | | |
2983 | | > select add #29.1 |
2984 | | |
2985 | | 9 models selected |
2986 | | |
2987 | | > select subtract #29.1 |
2988 | | |
2989 | | 7 models selected |
2990 | | |
2991 | | > select add #29.1 |
2992 | | |
2993 | | 9 models selected |
2994 | | |
2995 | | > select subtract #29.1 |
2996 | | |
2997 | | 7 models selected |
2998 | | |
2999 | | > color #29 darkgrey models |
3000 | | |
3001 | | > color #29 #a9a9a993 models |
3002 | | |
3003 | | > color #29 #a9a9a994 models |
3004 | | |
3005 | | > select add #29 |
3006 | | |
3007 | | 9 models selected |
3008 | | |
3009 | | > select subtract #29 |
3010 | | |
3011 | | Nothing selected |
3012 | | |
3013 | | > hide #!29 models |
3014 | | |
3015 | | > rename #29 W20_J140_MB |
3016 | | |
3017 | | > open "/Users/becca/Desktop/Postdoc/COPI notebooks and |
3018 | | > resources/Processing/W20_MM_cyto_COPI/relionMB/W20_J145_run_ct8_body001.mrc" |
3019 | | > "/Users/becca/Desktop/Postdoc/COPI notebooks and |
3020 | | > resources/Processing/W20_MM_cyto_COPI/relionMB/W20_J145_run_ct8_body002.mrc" |
3021 | | > "/Users/becca/Desktop/Postdoc/COPI notebooks and |
3022 | | > resources/Processing/W20_MM_cyto_COPI/relionMB/W20_J145_run_ct8_body003.mrc" |
3023 | | > "/Users/becca/Desktop/Postdoc/COPI notebooks and |
3024 | | > resources/Processing/W20_MM_cyto_COPI/relionMB/W20_J145_run_ct8_body004.mrc" |
3025 | | |
3026 | | Opened W20_J145_run_ct8_body001.mrc as #30.1, grid size 240,240,240, pixel |
3027 | | 1.2, shown at level 0.00616, step 1, values float32 |
3028 | | Opened W20_J145_run_ct8_body002.mrc as #30.2, grid size 240,240,240, pixel |
3029 | | 1.2, shown at level 0.00152, step 1, values float32 |
3030 | | Opened W20_J145_run_ct8_body003.mrc as #30.3, grid size 240,240,240, pixel |
3031 | | 1.2, shown at level 0.000716, step 1, values float32 |
3032 | | Opened W20_J145_run_ct8_body004.mrc as #30.4, grid size 240,240,240, pixel |
3033 | | 1.2, shown at level 0.000812, step 1, values float32 |
3034 | | |
3035 | | > select add #30 |
3036 | | |
3037 | | 9 models selected |
3038 | | |
3039 | | > ui mousemode right "rotate selected models" |
3040 | | |
3041 | | > view matrix models |
3042 | | > #30,-0.35278,-0.9274,-0.12442,348.28,-0.78005,0.21805,0.5865,134.81,-0.51679,0.30396,-0.80034,301.49 |
3043 | | |
3044 | | > ui mousemode right "translate selected models" |
3045 | | |
3046 | | > view matrix models |
3047 | | > #30,-0.35278,-0.9274,-0.12442,340.27,-0.78005,0.21805,0.5865,136.72,-0.51679,0.30396,-0.80034,288.45 |
3048 | | |
3049 | | > fitmap #30.4 inMap #1 |
3050 | | |
3051 | | Fit map W20_J145_run_ct8_body004.mrc in map |
3052 | | W20_J3623_consensus_structure_sharp.mrc using 137951 points |
3053 | | correlation = 0.7282, correlation about mean = 0.4288, overlap = 228.8 |
3054 | | steps = 112, shift = 11.5, angle = 9.2 degrees |
3055 | | |
3056 | | Position of W20_J145_run_ct8_body004.mrc (#30.4) relative to |
3057 | | W20_J3623_consensus_structure_sharp.mrc (#1) coordinates: |
3058 | | Matrix rotation and translation |
3059 | | 0.98788522 -0.14632868 -0.05167889 26.91126566 |
3060 | | 0.14822194 0.98833778 0.03490982 -21.95721425 |
3061 | | 0.04596789 -0.04214685 0.99805340 -1.50223115 |
3062 | | Axis -0.24099906 -0.30539579 0.92122357 |
3063 | | Axis point 163.78780037 168.43265871 0.00000000 |
3064 | | Rotation angle (degrees) 9.19930630 |
3065 | | Shift along axis -1.16383962 |
3066 | | |
3067 | | |
3068 | | > fitmap #30.3 inMap #1 |
3069 | | |
3070 | | Fit map W20_J145_run_ct8_body003.mrc in map |
3071 | | W20_J3623_consensus_structure_sharp.mrc using 137543 points |
3072 | | correlation = 0.6493, correlation about mean = 0.3323, overlap = 237.6 |
3073 | | steps = 116, shift = 8.58, angle = 1.99 degrees |
3074 | | |
3075 | | Position of W20_J145_run_ct8_body003.mrc (#30.3) relative to |
3076 | | W20_J3623_consensus_structure_sharp.mrc (#1) coordinates: |
3077 | | Matrix rotation and translation |
3078 | | 0.99943608 -0.00513122 -0.03318412 -0.25293108 |
3079 | | 0.00484442 0.99995027 -0.00871730 -4.33467984 |
3080 | | 0.03322720 0.00855163 0.99941124 -5.67738477 |
3081 | | Axis 0.24904308 -0.95774784 0.14386320 |
3082 | | Axis point 184.40566041 0.00000000 -35.31005024 |
3083 | | Rotation angle (degrees) 1.98687515 |
3084 | | Shift along axis 3.27177278 |
3085 | | |
3086 | | |
3087 | | > fitmap #30.2 inMap #1 |
3088 | | |
3089 | | Fit map W20_J145_run_ct8_body002.mrc in map |
3090 | | W20_J3623_consensus_structure_sharp.mrc using 137963 points |
3091 | | correlation = 0.8212, correlation about mean = 0.6198, overlap = 468.2 |
3092 | | steps = 92, shift = 7.64, angle = 3.06 degrees |
3093 | | |
3094 | | Position of W20_J145_run_ct8_body002.mrc (#30.2) relative to |
3095 | | W20_J3623_consensus_structure_sharp.mrc (#1) coordinates: |
3096 | | Matrix rotation and translation |
3097 | | 0.99870570 0.02334084 -0.04519002 -1.99430024 |
3098 | | -0.02260011 0.99960285 0.01683358 -2.36740438 |
3099 | | 0.04556498 -0.01579049 0.99883657 -3.32045770 |
3100 | | Axis -0.30540022 -0.84957484 -0.43006196 |
3101 | | Axis point 34.20968149 0.00000000 -17.75195541 |
3102 | | Rotation angle (degrees) 3.06173952 |
3103 | | Shift along axis 4.04834949 |
3104 | | |
3105 | | |
3106 | | > fitmap #30.1 inMap #1 |
3107 | | |
3108 | | Fit map W20_J145_run_ct8_body001.mrc in map |
3109 | | W20_J3623_consensus_structure_sharp.mrc using 138203 points |
3110 | | correlation = 0.9153, correlation about mean = 0.7173, overlap = 857.4 |
3111 | | steps = 84, shift = 6.27, angle = 2.89 degrees |
3112 | | |
3113 | | Position of W20_J145_run_ct8_body001.mrc (#30.1) relative to |
3114 | | W20_J3623_consensus_structure_sharp.mrc (#1) coordinates: |
3115 | | Matrix rotation and translation |
3116 | | 0.99917915 0.02581546 -0.03121838 -4.27539402 |
3117 | | -0.02486602 0.99922761 0.03042809 -4.63326697 |
3118 | | 0.03197979 -0.02962683 0.99904932 0.16478454 |
3119 | | Axis -0.59553173 -0.62670160 -0.50258041 |
3120 | | Axis point -90.45500707 0.00000000 -32.19034476 |
3121 | | Rotation angle (degrees) 2.89015105 |
3122 | | Shift along axis 5.36699111 |
3123 | | |
3124 | | |
3125 | | > color #30 darkgrey models |
3126 | | |
3127 | | > color #30 #a9a9a980 models |
3128 | | |
3129 | | > select subtract #30 |
3130 | | |
3131 | | Nothing selected |
3132 | | |
3133 | | > hide #!30 models |
3134 | | |
3135 | | > rename #30 W20_J145_MB |
3136 | | |
3137 | | > open "/Users/becca/Desktop/Postdoc/COPI notebooks and |
3138 | | > resources/Processing/W20_MM_cyto_COPI/relionMB/W20_J150_run_body001.mrc" |
3139 | | > "/Users/becca/Desktop/Postdoc/COPI notebooks and |
3140 | | > resources/Processing/W20_MM_cyto_COPI/relionMB/W20_J150_run_body002.mrc" |
3141 | | > "/Users/becca/Desktop/Postdoc/COPI notebooks and |
3142 | | > resources/Processing/W20_MM_cyto_COPI/relionMB/W20_J150_run_body003.mrc" |
3143 | | > "/Users/becca/Desktop/Postdoc/COPI notebooks and |
3144 | | > resources/Processing/W20_MM_cyto_COPI/relionMB/W20_J150_run_body004.mrc" |
3145 | | |
3146 | | Opened W20_J150_run_body001.mrc as #31.1, grid size 240,240,240, pixel 1.2, |
3147 | | shown at level 0.0066, step 1, values float32 |
3148 | | Opened W20_J150_run_body002.mrc as #31.2, grid size 240,240,240, pixel 1.2, |
3149 | | shown at level 0.0013, step 1, values float32 |
3150 | | Opened W20_J150_run_body003.mrc as #31.3, grid size 240,240,240, pixel 1.2, |
3151 | | shown at level 0.000798, step 1, values float32 |
3152 | | Opened W20_J150_run_body004.mrc as #31.4, grid size 240,240,240, pixel 1.2, |
3153 | | shown at level 0.000426, step 1, values float32 |
3154 | | |
3155 | | > select add #31 |
3156 | | |
3157 | | 9 models selected |
3158 | | |
3159 | | > ui mousemode right "rotate selected models" |
3160 | | |
3161 | | > view matrix models |
3162 | | > #31,0.62778,0.15918,-0.76194,138.56,0.19756,0.91423,0.35377,-64.97,0.7529,-0.37262,0.54248,29.724 |
3163 | | |
3164 | | > view matrix models |
3165 | | > #31,-0.3321,-0.94322,0.0062597,329.8,-0.75269,0.269,0.60092,127.95,-0.56848,0.19485,-0.79929,311.9 |
3166 | | |
3167 | | > ui mousemode right "translate selected models" |
3168 | | |
3169 | | > view matrix models |
3170 | | > #31,-0.3321,-0.94322,0.0062597,332.22,-0.75269,0.269,0.60092,127.27,-0.56848,0.19485,-0.79929,317.96 |
3171 | | |
3172 | | > fitmap #31.1 inMap #1 |
3173 | | |
3174 | | Fit map W20_J150_run_body001.mrc in map |
3175 | | W20_J3623_consensus_structure_sharp.mrc using 138218 points |
3176 | | correlation = 0.9172, correlation about mean = 0.7038, overlap = 893.5 |
3177 | | steps = 88, shift = 6.98, angle = 8.67 degrees |
3178 | | |
3179 | | Position of W20_J150_run_body001.mrc (#31.1) relative to |
3180 | | W20_J3623_consensus_structure_sharp.mrc (#1) coordinates: |
3181 | | Matrix rotation and translation |
3182 | | 0.99964242 -0.02165534 0.01568687 5.92000022 |
3183 | | 0.01908723 0.98871756 0.14857073 -17.88200850 |
3184 | | -0.01872723 -0.14821819 0.98877736 29.33526708 |
3185 | | Axis -0.98423540 0.11412684 0.13511379 |
3186 | | Axis point 0.00000000 191.49090363 131.90319589 |
3187 | | Rotation angle (degrees) 8.67162751 |
3188 | | Shift along axis -3.90389179 |
3189 | | |
3190 | | |
3191 | | > fitmap #31.2 inMap #1 |
3192 | | |
3193 | | Fit map W20_J150_run_body002.mrc in map |
3194 | | W20_J3623_consensus_structure_sharp.mrc using 138140 points |
3195 | | correlation = 0.8053, correlation about mean = 0.5992, overlap = 456.9 |
3196 | | steps = 116, shift = 12.3, angle = 8.44 degrees |
3197 | | |
3198 | | Position of W20_J150_run_body002.mrc (#31.2) relative to |
3199 | | W20_J3623_consensus_structure_sharp.mrc (#1) coordinates: |
3200 | | Matrix rotation and translation |
3201 | | 0.99987004 -0.01303295 0.00948916 5.64171510 |
3202 | | 0.01150966 0.98923255 0.14589889 -15.99717561 |
3203 | | -0.01128848 -0.14577072 0.98925400 27.73858221 |
3204 | | Axis -0.99397729 0.07080790 0.08363845 |
3205 | | Axis point 0.00000000 183.90016756 121.07225767 |
3206 | | Rotation angle (degrees) 8.43680332 |
3207 | | Shift along axis -4.42045109 |
3208 | | |
3209 | | |
3210 | | > fitmap #31.3 inMap #1 |
3211 | | |
3212 | | Fit map W20_J150_run_body003.mrc in map |
3213 | | W20_J3623_consensus_structure_sharp.mrc using 137514 points |
3214 | | correlation = 0.6146, correlation about mean = 0.266, overlap = 238.9 |
3215 | | steps = 124, shift = 9.37, angle = 6.56 degrees |
3216 | | |
3217 | | Position of W20_J150_run_body003.mrc (#31.3) relative to |
3218 | | W20_J3623_consensus_structure_sharp.mrc (#1) coordinates: |
3219 | | Matrix rotation and translation |
3220 | | 0.99927585 -0.03094085 0.02214582 5.52526341 |
3221 | | 0.02837142 0.99372585 0.10818506 -14.70532330 |
3222 | | -0.02535421 -0.10747841 0.99388408 23.92950955 |
3223 | | Axis -0.94316639 0.20773307 0.25939184 |
3224 | | Axis point 0.00000000 219.38003792 144.69692071 |
3225 | | Rotation angle (degrees) 6.56495264 |
3226 | | Shift along axis -2.05890526 |
3227 | | |
3228 | | |
3229 | | > fitmap #31.4 inMap #1 |
3230 | | |
3231 | | Fit map W20_J150_run_body004.mrc in map |
3232 | | W20_J3623_consensus_structure_sharp.mrc using 137710 points |
3233 | | correlation = 0.7262, correlation about mean = 0.4714, overlap = 200 |
3234 | | steps = 100, shift = 8.31, angle = 7.56 degrees |
3235 | | |
3236 | | Position of W20_J150_run_body004.mrc (#31.4) relative to |
3237 | | W20_J3623_consensus_structure_sharp.mrc (#1) coordinates: |
3238 | | Matrix rotation and translation |
3239 | | 0.99968731 -0.01039845 0.02274087 4.61807189 |
3240 | | 0.00737128 0.99156997 0.12936254 -14.95364310 |
3241 | | -0.02389433 -0.12915446 0.99133656 25.99366724 |
3242 | | Axis -0.98187156 0.17712480 0.06749108 |
3243 | | Axis point 0.00000000 195.98514242 120.93339845 |
3244 | | Rotation angle (degrees) 7.56466213 |
3245 | | Shift along axis -5.42867381 |
3246 | | |
3247 | | |
3248 | | > color #31 darkgrey models |
3249 | | |
3250 | | > color #31 #a9a9a980 models |
3251 | | |
3252 | | > select subtract #31 |
3253 | | |
3254 | | Nothing selected |
3255 | | |
3256 | | > hide #!31 models |
3257 | | |
3258 | | > rename #31 W20_J150_MB |
3259 | | |
3260 | | > open "/Users/becca/Desktop/Postdoc/COPI notebooks and |
3261 | | > resources/Processing/W20_MM_cyto_COPI/relionMB/W20_J155_run_ct6_body001.mrc" |
3262 | | > "/Users/becca/Desktop/Postdoc/COPI notebooks and |
3263 | | > resources/Processing/W20_MM_cyto_COPI/relionMB/W20_J155_run_ct6_body002.mrc" |
3264 | | > "/Users/becca/Desktop/Postdoc/COPI notebooks and |
3265 | | > resources/Processing/W20_MM_cyto_COPI/relionMB/W20_J155_run_ct6_body003.mrc" |
3266 | | > "/Users/becca/Desktop/Postdoc/COPI notebooks and |
3267 | | > resources/Processing/W20_MM_cyto_COPI/relionMB/W20_J155_run_ct6_body004.mrc" |
3268 | | |
3269 | | Opened W20_J155_run_ct6_body001.mrc as #32.1, grid size 240,240,240, pixel |
3270 | | 1.2, shown at level 0.0064, step 1, values float32 |
3271 | | Opened W20_J155_run_ct6_body002.mrc as #32.2, grid size 240,240,240, pixel |
3272 | | 1.2, shown at level 0.00156, step 1, values float32 |
3273 | | Opened W20_J155_run_ct6_body003.mrc as #32.3, grid size 240,240,240, pixel |
3274 | | 1.2, shown at level 0.000666, step 1, values float32 |
3275 | | Opened W20_J155_run_ct6_body004.mrc as #32.4, grid size 240,240,240, pixel |
3276 | | 1.2, shown at level 0.000439, step 1, values float32 |
3277 | | |
3278 | | > select add #32 |
3279 | | |
3280 | | 9 models selected |
3281 | | |
3282 | | > ui mousemode right "rotate selected models" |
3283 | | |
3284 | | > view matrix models |
3285 | | > #32,-0.094929,-0.99244,0.07775,390.6,-0.44492,0.11217,0.88852,96.076,-0.89053,0.049754,-0.45221,395 |
3286 | | |
3287 | | > ui mousemode right "translate selected models" |
3288 | | |
3289 | | > view matrix models |
3290 | | > #32,-0.094929,-0.99244,0.07775,372.3,-0.44492,0.11217,0.88852,92.438,-0.89053,0.049754,-0.45221,335.63 |
3291 | | |
3292 | | > ui mousemode right "translate selected models" |
3293 | | |
3294 | | > view matrix models |
3295 | | > #32,-0.094929,-0.99244,0.07775,368.31,-0.44492,0.11217,0.88852,91.761,-0.89053,0.049754,-0.45221,322.87 |
3296 | | |
3297 | | > ui mousemode right "rotate selected models" |
3298 | | |
3299 | | > view matrix models |
3300 | | > #32,0.24986,-0.92724,-0.27892,355.61,-0.7966,-0.36061,0.48518,310.1,-0.55046,0.10096,-0.82874,322.22 |
3301 | | |
3302 | | > ui mousemode right "translate selected models" |
3303 | | |
3304 | | > view matrix models |
3305 | | > #32,0.24986,-0.92724,-0.27892,326.75,-0.7966,-0.36061,0.48518,243.97,-0.55046,0.10096,-0.82874,318.12 |
3306 | | |
3307 | | > ui mousemode right "rotate selected models" |
3308 | | |
3309 | | > view matrix models |
3310 | | > #32,0.049187,-0.95013,-0.30796,371.28,-0.76011,-0.23563,0.60556,193.56,-0.64792,0.2043,-0.7338,299.66 |
3311 | | |
3312 | | > view matrix models |
3313 | | > #32,-0.38216,-0.71778,-0.58202,446.74,-0.57887,-0.30499,0.75623,147.95,-0.72032,0.62591,-0.29895,168.04 |
3314 | | |
3315 | | > view matrix models |
3316 | | > #32,-0.12174,-0.98206,-0.14398,378.68,-0.79719,0.01032,0.60364,153.76,-0.59133,0.18827,-0.78414,308.73 |
3317 | | |
3318 | | > ui mousemode right "translate selected models" |
3319 | | |
3320 | | > view matrix models |
3321 | | > #32,-0.12174,-0.98206,-0.14398,339.88,-0.79719,0.01032,0.60364,183.81,-0.59133,0.18827,-0.78414,311.67 |
3322 | | |
3323 | | > view matrix models |
3324 | | > #32,-0.12174,-0.98206,-0.14398,341.73,-0.79719,0.01032,0.60364,179.24,-0.59133,0.18827,-0.78414,317.95 |
3325 | | |
3326 | | > fitmap #32.4 inMap #1 |
3327 | | |
3328 | | Fit map W20_J155_run_ct6_body004.mrc in map |
3329 | | W20_J3623_consensus_structure_sharp.mrc using 137851 points |
3330 | | correlation = 0.7818, correlation about mean = 0.5257, overlap = 192.1 |
3331 | | steps = 124, shift = 4.21, angle = 14.8 degrees |
3332 | | |
3333 | | Position of W20_J155_run_ct6_body004.mrc (#32.4) relative to |
3334 | | W20_J3623_consensus_structure_sharp.mrc (#1) coordinates: |
3335 | | Matrix rotation and translation |
3336 | | 0.96755911 -0.24974155 0.03819070 46.04596253 |
3337 | | 0.24817341 0.96782709 0.04148106 -19.98854272 |
3338 | | -0.04732154 -0.03065746 0.99840913 9.25104536 |
3339 | | Axis -0.14135691 0.16756298 0.97567457 |
3340 | | Axis point 102.10235956 170.64051821 0.00000000 |
3341 | | Rotation angle (degrees) 14.78333075 |
3342 | | Shift along axis -0.83224502 |
3343 | | |
3344 | | |
3345 | | > fitmap #32.3 inMap #1 |
3346 | | |
3347 | | Fit map W20_J155_run_ct6_body003.mrc in map |
3348 | | W20_J3623_consensus_structure_sharp.mrc using 137790 points |
3349 | | correlation = 0.5288, correlation about mean = 0.2458, overlap = 222.1 |
3350 | | steps = 212, shift = 24.3, angle = 25.3 degrees |
3351 | | |
3352 | | Position of W20_J155_run_ct6_body003.mrc (#32.3) relative to |
3353 | | W20_J3623_consensus_structure_sharp.mrc (#1) coordinates: |
3354 | | Matrix rotation and translation |
3355 | | 0.96236345 -0.02996556 0.27010858 -46.55042113 |
3356 | | -0.06370212 0.94133951 0.33139398 -51.24613942 |
3357 | | -0.26419429 -0.33612794 0.90400187 97.79261178 |
3358 | | Axis -0.78009899 0.62441264 -0.03942621 |
3359 | | Axis point 0.00000000 234.71472833 197.05127274 |
3360 | | Rotation angle (degrees) 25.33083616 |
3361 | | Shift along axis 0.45960726 |
3362 | | |
3363 | | |
3364 | | > fitmap #32.2 inMap #1 |
3365 | | |
3366 | | Fit map W20_J155_run_ct6_body002.mrc in map |
3367 | | W20_J3623_consensus_structure_sharp.mrc using 138167 points |
3368 | | correlation = 0.8427, correlation about mean = 0.6574, overlap = 508.2 |
3369 | | steps = 200, shift = 28.2, angle = 13.9 degrees |
3370 | | |
3371 | | Position of W20_J155_run_ct6_body002.mrc (#32.2) relative to |
3372 | | W20_J3623_consensus_structure_sharp.mrc (#1) coordinates: |
3373 | | Matrix rotation and translation |
3374 | | 0.97079416 -0.23981830 0.00677387 48.62036376 |
3375 | | 0.23979745 0.97081570 0.00375114 -13.12837058 |
3376 | | -0.00747577 -0.00201723 0.99997002 1.33700507 |
3377 | | Axis -0.01202088 0.02969530 0.99948671 |
3378 | | Axis point 78.30820657 193.21986153 0.00000000 |
3379 | | Rotation angle (degrees) 13.88246944 |
3380 | | Shift along axis 0.36200848 |
3381 | | |
3382 | | |
3383 | | > fitmap #32.1 inMap #1 |
3384 | | |
3385 | | Fit map W20_J155_run_ct6_body001.mrc in map |
3386 | | W20_J3623_consensus_structure_sharp.mrc using 138158 points |
3387 | | correlation = 0.9152, correlation about mean = 0.7176, overlap = 887.9 |
3388 | | steps = 240, shift = 17.9, angle = 14.3 degrees |
3389 | | |
3390 | | Position of W20_J155_run_ct6_body001.mrc (#32.1) relative to |
3391 | | W20_J3623_consensus_structure_sharp.mrc (#1) coordinates: |
3392 | | Matrix rotation and translation |
3393 | | 0.96918769 -0.24592134 0.01406818 48.78106404 |
3394 | | 0.24563656 0.96916974 0.01930526 -16.53168875 |
3395 | | -0.01838203 -0.01525477 0.99971466 4.87447888 |
3396 | | Axis -0.06998243 0.06571014 0.99538165 |
3397 | | Axis point 90.87557164 187.07445802 0.00000000 |
3398 | | Rotation angle (degrees) 14.29528730 |
3399 | | Shift along axis 0.35184982 |
3400 | | |
3401 | | |
3402 | | > select add #32.3 |
3403 | | |
3404 | | 9 models selected |
3405 | | |
3406 | | > select subtract #32 |
3407 | | |
3408 | | Nothing selected |
3409 | | |
3410 | | > select subtract #32.3 |
3411 | | |
3412 | | Nothing selected |
3413 | | |
3414 | | > select add #32.3 |
3415 | | |
3416 | | 2 models selected |
3417 | | |
3418 | | > view matrix models |
3419 | | > #32.3,0.96236,-0.029966,0.27011,-49.146,-0.063702,0.94134,0.33139,-16.537,-0.26419,-0.33613,0.904,117.55 |
3420 | | |
3421 | | > fitmap #32.3 inMap #1 |
3422 | | |
3423 | | Fit map W20_J155_run_ct6_body003.mrc in map |
3424 | | W20_J3623_consensus_structure_sharp.mrc using 137790 points |
3425 | | correlation = 0.5773, correlation about mean = 0.2332, overlap = 255.7 |
3426 | | steps = 220, shift = 22.6, angle = 31.2 degrees |
3427 | | |
3428 | | Position of W20_J155_run_ct6_body003.mrc (#32.3) relative to |
3429 | | W20_J3623_consensus_structure_sharp.mrc (#1) coordinates: |
3430 | | Matrix rotation and translation |
3431 | | 0.93701301 -0.34494790 0.05493240 55.45886688 |
3432 | | 0.34457856 0.93861493 0.01635939 -26.98464957 |
3433 | | -0.05720351 0.00359956 0.99835605 3.33562233 |
3434 | | Axis -0.01826220 0.16049183 0.98686821 |
3435 | | Axis point 102.63951914 141.92416208 0.00000000 |
3436 | | Rotation angle (degrees) 20.44761418 |
3437 | | Shift along axis -2.05179723 |
3438 | | |
3439 | | |
3440 | | > select subtract #32.3 |
3441 | | |
3442 | | Nothing selected |
3443 | | |
3444 | | > color #32 #929292ff models |
3445 | | |
3446 | | > color #32 #9292927d models |
3447 | | |
3448 | | > hide #!32 models |
3449 | | |
3450 | | > rename #32 W20_J155_MB |
3451 | | |
3452 | | > open "/Users/becca/Desktop/Postdoc/COPI notebooks and |
3453 | | > resources/Processing/W20_MM_cyto_COPI/relionMB/W20_J160_run_ct6_body001.mrc" |
3454 | | > "/Users/becca/Desktop/Postdoc/COPI notebooks and |
3455 | | > resources/Processing/W20_MM_cyto_COPI/relionMB/W20_J160_run_ct6_body002.mrc" |
3456 | | > "/Users/becca/Desktop/Postdoc/COPI notebooks and |
3457 | | > resources/Processing/W20_MM_cyto_COPI/relionMB/W20_J160_run_ct6_body003.mrc" |
3458 | | > "/Users/becca/Desktop/Postdoc/COPI notebooks and |
3459 | | > resources/Processing/W20_MM_cyto_COPI/relionMB/W20_J160_run_ct6_body004.mrc" |
3460 | | |
3461 | | Opened W20_J160_run_ct6_body001.mrc as #33.1, grid size 240,240,240, pixel |
3462 | | 1.2, shown at level 0.00725, step 1, values float32 |
3463 | | Opened W20_J160_run_ct6_body002.mrc as #33.2, grid size 240,240,240, pixel |
3464 | | 1.2, shown at level 0.00154, step 1, values float32 |
3465 | | Opened W20_J160_run_ct6_body003.mrc as #33.3, grid size 240,240,240, pixel |
3466 | | 1.2, shown at level 0.000864, step 1, values float32 |
3467 | | Opened W20_J160_run_ct6_body004.mrc as #33.4, grid size 240,240,240, pixel |
3468 | | 1.2, shown at level 0.000379, step 1, values float32 |
3469 | | |
3470 | | > select add #33 |
3471 | | |
3472 | | 9 models selected |
3473 | | |
3474 | | > ui mousemode right "rotate selected models" |
3475 | | |
3476 | | > view matrix models |
3477 | | > #33,0.31873,-0.82608,0.46476,171.28,-0.72157,0.10647,0.6841,150.82,-0.6146,-0.5534,-0.56214,456.98 |
3478 | | |
3479 | | > view matrix models |
3480 | | > #33,-0.036679,-0.99898,-0.026385,337.23,-0.84584,0.016974,0.53317,211.08,-0.53218,0.041873,-0.8456,383.69 |
3481 | | |
3482 | | > ui mousemode right "translate selected models" |
3483 | | |
3484 | | > view matrix models |
3485 | | > #33,-0.036679,-0.99898,-0.026385,334.88,-0.84584,0.016974,0.53317,195.64,-0.53218,0.041873,-0.8456,333.69 |
3486 | | |
3487 | | > view matrix models |
3488 | | > #33,-0.036679,-0.99898,-0.026385,313.34,-0.84584,0.016974,0.53317,188.83,-0.53218,0.041873,-0.8456,328.42 |
3489 | | |
3490 | | > view matrix models |
3491 | | > #33,-0.036679,-0.99898,-0.026385,311.41,-0.84584,0.016974,0.53317,189.7,-0.53218,0.041873,-0.8456,344.34 |
3492 | | |
3493 | | > ui mousemode right "rotate selected models" |
3494 | | |
3495 | | > view matrix models |
3496 | | > #33,-0.35879,-0.93341,-0.0038469,340.33,-0.80584,0.30767,0.50593,135.86,-0.47106,0.18462,-0.86256,313.27 |
3497 | | |
3498 | | > ui mousemode right "translate selected models" |
3499 | | |
3500 | | > view matrix models |
3501 | | > #33,-0.35879,-0.93341,-0.0038469,338.34,-0.80584,0.30767,0.50593,141.61,-0.47106,0.18462,-0.86256,318.7 |
3502 | | |
3503 | | > fitmap #33.3 inMap #1 |
3504 | | |
3505 | | Fit map W20_J160_run_ct6_body003.mrc in map |
3506 | | W20_J3623_consensus_structure_sharp.mrc using 137937 points |
3507 | | correlation = 0.5867, correlation about mean = 0.2346, overlap = 250.3 |
3508 | | steps = 140, shift = 11.6, angle = 7.35 degrees |
3509 | | |
3510 | | Position of W20_J160_run_ct6_body003.mrc (#33.3) relative to |
3511 | | W20_J3623_consensus_structure_sharp.mrc (#1) coordinates: |
3512 | | Matrix rotation and translation |
3513 | | 0.99303756 0.05988139 0.10144267 -23.49545369 |
3514 | | -0.06488899 0.99679433 0.04680249 14.70011589 |
3515 | | -0.09831488 -0.05305914 0.99373986 28.54412294 |
3516 | | Axis -0.39036137 0.78085672 -0.48773024 |
3517 | | Axis point 309.26276930 0.00000000 226.94693512 |
3518 | | Rotation angle (degrees) 7.34879054 |
3519 | | Shift along axis 6.72856998 |
3520 | | |
3521 | | |
3522 | | > fitmap #33.2 inMap #1 |
3523 | | |
3524 | | Fit map W20_J160_run_ct6_body002.mrc in map |
3525 | | W20_J3623_consensus_structure_sharp.mrc using 137991 points |
3526 | | correlation = 0.819, correlation about mean = 0.6067, overlap = 480.4 |
3527 | | steps = 144, shift = 18.2, angle = 13.8 degrees |
3528 | | |
3529 | | Position of W20_J160_run_ct6_body002.mrc (#33.2) relative to |
3530 | | W20_J3623_consensus_structure_sharp.mrc (#1) coordinates: |
3531 | | Matrix rotation and translation |
3532 | | 0.99771963 -0.01489642 -0.06583042 17.27645423 |
3533 | | 0.02956838 0.97323369 0.22790766 -26.83710836 |
3534 | | 0.06067337 -0.22933444 0.97145481 35.68296509 |
3535 | | Axis -0.95958803 -0.26548631 0.09331575 |
3536 | | Axis point 0.00000000 140.48687556 141.38690851 |
3537 | | Rotation angle (degrees) 13.78322772 |
3538 | | Shift along axis -6.12361124 |
3539 | | |
3540 | | |
3541 | | > fitmap #33.1 inMap #1 |
3542 | | |
3543 | | Fit map W20_J160_run_ct6_body001.mrc in map |
3544 | | W20_J3623_consensus_structure_sharp.mrc using 138219 points |
3545 | | correlation = 0.9149, correlation about mean = 0.7061, overlap = 905.5 |
3546 | | steps = 240, shift = 9.92, angle = 13.4 degrees |
3547 | | |
3548 | | Position of W20_J160_run_ct6_body001.mrc (#33.1) relative to |
3549 | | W20_J3623_consensus_structure_sharp.mrc (#1) coordinates: |
3550 | | Matrix rotation and translation |
3551 | | 0.99744755 -0.02103927 -0.06823292 18.77482829 |
3552 | | 0.03561801 0.97482901 0.22009032 -27.49182319 |
3553 | | 0.06188489 -0.22195888 0.97309019 34.85093721 |
3554 | | Axis -0.95213476 -0.28026222 0.12203477 |
3555 | | Axis point 0.00000000 142.23926756 148.71521434 |
3556 | | Rotation angle (degrees) 13.42285005 |
3557 | | Shift along axis -5.91822131 |
3558 | | |
3559 | | |
3560 | | > fitmap #33.4 inMap #1 |
3561 | | |
3562 | | Fit map W20_J160_run_ct6_body004.mrc in map |
3563 | | W20_J3623_consensus_structure_sharp.mrc using 137797 points |
3564 | | correlation = 0.7345, correlation about mean = 0.436, overlap = 185.3 |
3565 | | steps = 144, shift = 10.1, angle = 13 degrees |
3566 | | |
3567 | | Position of W20_J160_run_ct6_body004.mrc (#33.4) relative to |
3568 | | W20_J3623_consensus_structure_sharp.mrc (#1) coordinates: |
3569 | | Matrix rotation and translation |
3570 | | 0.99800988 0.00249027 -0.06300862 14.90861204 |
3571 | | 0.01124609 0.97617108 0.21671073 -24.80457915 |
3572 | | 0.06204686 -0.21698805 0.97420038 33.68609528 |
3573 | | Axis -0.96067232 -0.27700640 0.01939474 |
3574 | | Axis point 0.00000000 139.38706533 138.47533972 |
3575 | | Rotation angle (degrees) 13.04561440 |
3576 | | Shift along axis -6.79793059 |
3577 | | |
3578 | | |
3579 | | > select subtract #33 |
3580 | | |
3581 | | Nothing selected |
3582 | | |
3583 | | > color #33 #929292ff models |
3584 | | |
3585 | | > color #33 #92929280 models |
3586 | | |
3587 | | > hide #!33 models |
3588 | | |
3589 | | > rename #33 W20_J160_MB |
3590 | | |
3591 | | > save "/Users/becca/Desktop/Postdoc/COPI notebooks and |
3592 | | > resources/Processing/W20_MM_cyto_COPI/20250701_W20_COPI_cyto_alignments.cxs" |
3593 | | |
3594 | | ——— End of log from Tue Jul 1 13:02:54 2025 ——— |
3595 | | |
3596 | | opened ChimeraX session |
3597 | | |
3598 | | > open "/Users/becca/Desktop/Postdoc/COPI notebooks and resources/Structure |
3599 | | > files/alphafold/CopA-F8WHL2.pdb" |
3600 | | |
3601 | | CopA-F8WHL2.pdb title: |
3602 | | Alphafold monomer V2.0 prediction for coatomer subunit α (F8WHL2) [more |
3603 | | info...] |
3604 | | |
3605 | | Chain information for CopA-F8WHL2.pdb #34 |
3606 | | --- |
3607 | | Chain | Description | UniProt |
3608 | | A | coatomer subunit α | F8WHL2_MOUSE 1-1233 |
3609 | | |
3610 | | Computing secondary structure |
3611 | | |
3612 | | > open "/Users/becca/Desktop/Postdoc/COPI notebooks and resources/Structure |
3613 | | > files/alphafold/CopB_ Q9JIF7.pdb" |
3614 | | |
3615 | | CopB_ Q9JIF7.pdb title: |
3616 | | Alphafold monomer V2.0 prediction for coatomer subunit β (Q9JIF7) [more |
3617 | | info...] |
3618 | | |
3619 | | Chain information for CopB_ Q9JIF7.pdb #35 |
3620 | | --- |
3621 | | Chain | Description | UniProt |
3622 | | A | coatomer subunit β | COPB_MOUSE 1-953 |
3623 | | |
3624 | | Computing secondary structure |
3625 | | |
3626 | | > open "/Users/becca/Desktop/Postdoc/COPI notebooks and resources/Structure |
3627 | | > files/alphafold/CopBprime_O55029.pdb" |
3628 | | |
3629 | | CopBprime_O55029.pdb title: |
3630 | | Alphafold monomer V2.0 prediction for coatomer subunit β' (O55029) [more |
3631 | | info...] |
3632 | | |
3633 | | Chain information for CopBprime_O55029.pdb #36 |
3634 | | --- |
3635 | | Chain | Description | UniProt |
3636 | | A | coatomer subunit β' | COPB2_MOUSE 1-905 |
3637 | | |
3638 | | Computing secondary structure |
3639 | | |
3640 | | > open "/Users/becca/Desktop/Postdoc/COPI notebooks and resources/Structure |
3641 | | > files/alphafold/CopD_full_Q5XJY5.pdb" |
3642 | | |
3643 | | CopD_full_Q5XJY5.pdb title: |
3644 | | Alphafold monomer V2.0 prediction for coatomer subunit δ (Q5XJY5) [more |
3645 | | info...] |
3646 | | |
3647 | | Chain information for CopD_full_Q5XJY5.pdb #37 |
3648 | | --- |
3649 | | Chain | Description | UniProt |
3650 | | A | coatomer subunit δ | COPD_MOUSE 1-511 |
3651 | | |
3652 | | Computing secondary structure |
3653 | | |
3654 | | > open "/Users/becca/Desktop/Postdoc/COPI notebooks and resources/Structure |
3655 | | > files/alphafold/CopG1_ Q9QZE5.pdb" |
3656 | | |
3657 | | CopG1_ Q9QZE5.pdb title: |
3658 | | Alphafold monomer V2.0 prediction for coatomer subunit γ-1 (Q9QZE5) [more |
3659 | | info...] |
3660 | | |
3661 | | Chain information for CopG1_ Q9QZE5.pdb #38 |
3662 | | --- |
3663 | | Chain | Description | UniProt |
3664 | | A | coatomer subunit γ-1 | COPG1_MOUSE 1-874 |
3665 | | |
3666 | | Computing secondary structure |
3667 | | |
3668 | | > open "/Users/becca/Desktop/Postdoc/COPI notebooks and resources/Structure |
3669 | | > files/alphafold/CopG1_ Q9QZE5.pdb" |
3670 | | |
3671 | | CopG1_ Q9QZE5.pdb title: |
3672 | | Alphafold monomer V2.0 prediction for coatomer subunit γ-1 (Q9QZE5) [more |
3673 | | info...] |
3674 | | |
3675 | | Chain information for CopG1_ Q9QZE5.pdb #39 |
3676 | | --- |
3677 | | Chain | Description | UniProt |
3678 | | A | coatomer subunit γ-1 | COPG1_MOUSE 1-874 |
3679 | | |
3680 | | Computing secondary structure |
3681 | | |
3682 | | > open "/Users/becca/Desktop/Postdoc/COPI notebooks and resources/Structure |
3683 | | > files/alphafold/CopZ1_P61924.pdb" |
3684 | | |
3685 | | CopZ1_P61924.pdb title: |
3686 | | Alphafold monomer V2.0 prediction for coatomer subunit ζ-1 (P61924) [more |
3687 | | info...] |
3688 | | |
3689 | | Chain information for CopZ1_P61924.pdb #40 |
3690 | | --- |
3691 | | Chain | Description | UniProt |
3692 | | A | coatomer subunit ζ-1 | COPZ1_MOUSE 1-177 |
3693 | | |
3694 | | Computing secondary structure |
3695 | | |
3696 | | > hide #40 models |
3697 | | |
3698 | | > hide #39 models |
3699 | | |
3700 | | > hide #38 models |
3701 | | |
3702 | | > hide #37 models |
3703 | | |
3704 | | > hide #36 models |
3705 | | |
3706 | | > hide #35 models |
3707 | | |
3708 | | > select add #34 |
3709 | | |
3710 | | 9810 atoms, 10032 bonds, 1233 residues, 1 model selected |
3711 | | |
3712 | | > color list |
3713 | | |
3714 | | 28 custom colors: alphav1 |
3715 | | |
3716 | | , alphav2 |
3717 | | |
3718 | | , alphav3 |
3719 | | |
3720 | | , arf1v1 |
3721 | | |
3722 | | , arf1v2 |
3723 | | |
3724 | | , arf1v3 |
3725 | | |
3726 | | , betapv1 |
3727 | | |
3728 | | , betapv2 |
3729 | | |
3730 | | , betapv3 |
3731 | | |
3732 | | , betav1 |
3733 | | |
3734 | | , betav2 |
3735 | | |
3736 | | , betav3 |
3737 | | |
3738 | | , deltav1 |
3739 | | |
3740 | | , deltav2 |
3741 | | |
3742 | | , deltav3 |
3743 | | |
3744 | | , epsilonv1 |
3745 | | |
3746 | | , epsilonv3 |
3747 | | |
3748 | | , gammav1 |
3749 | | |
3750 | | , gammav2 |
3751 | | |
3752 | | , gammav3 |
3753 | | |
3754 | | , label_purple |
3755 | | |
3756 | | , label_purple_v3 |
3757 | | |
3758 | | , label_red |
3759 | | |
3760 | | , label_red_v3 |
3761 | | |
3762 | | , lable_purple_v4 |
3763 | | |
3764 | | , zetav1 |
3765 | | |
3766 | | , zetav2 |
3767 | | |
3768 | | , and zetav3 |
3769 | | |
3770 | | . |
3771 | | 248 builtin colors: alice blue |
3772 | | |
3773 | | , aliceblue |
3774 | | |
3775 | | , antique white |
3776 | | |
3777 | | , antiquewhite |
3778 | | |
3779 | | , aqua |
3780 | | |
3781 | | , aquamarine |
3782 | | |
3783 | | , azure |
3784 | | |
3785 | | , beige |
3786 | | |
3787 | | , bisque |
3788 | | |
3789 | | , black |
3790 | | |
3791 | | , blanched almond |
3792 | | |
3793 | | , blanchedalmond |
3794 | | |
3795 | | , blue |
3796 | | |
3797 | | , blue violet |
3798 | | |
3799 | | , blueviolet |
3800 | | |
3801 | | , brown |
3802 | | |
3803 | | , burly wood |
3804 | | |
3805 | | , burlywood |
3806 | | |
3807 | | , cadet blue |
3808 | | |
3809 | | , cadetblue |
3810 | | |
3811 | | , chartreuse |
3812 | | |
3813 | | , chocolate |
3814 | | |
3815 | | , coral |
3816 | | |
3817 | | , cornflower blue |
3818 | | |
3819 | | , cornflowerblue |
3820 | | |
3821 | | , cornsilk |
3822 | | |
3823 | | , crimson |
3824 | | |
3825 | | , cyan |
3826 | | |
3827 | | , dark blue |
3828 | | |
3829 | | , dark cyan |
3830 | | |
3831 | | , dark goldenrod |
3832 | | |
3833 | | , dark gray |
3834 | | |
3835 | | , dark green |
3836 | | |
3837 | | , dark grey |
3838 | | |
3839 | | , dark khaki |
3840 | | |
3841 | | , dark magenta |
3842 | | |
3843 | | , dark olive green |
3844 | | |
3845 | | , dark orange |
3846 | | |
3847 | | , dark orchid |
3848 | | |
3849 | | , dark red |
3850 | | |
3851 | | , dark salmon |
3852 | | |
3853 | | , dark sea green |
3854 | | |
3855 | | , dark seagreen |
3856 | | |
3857 | | , dark slate blue |
3858 | | |
3859 | | , dark slate gray |
3860 | | |
3861 | | , dark slate grey |
3862 | | |
3863 | | , dark turquoise |
3864 | | |
3865 | | , dark violet |
3866 | | |
3867 | | , darkblue |
3868 | | |
3869 | | , darkcyan |
3870 | | |
3871 | | , darkgoldenrod |
3872 | | |
3873 | | , darkgray |
3874 | | |
3875 | | , darkgreen |
3876 | | |
3877 | | , darkgrey |
3878 | | |
3879 | | , darkkhaki |
3880 | | |
3881 | | , darkmagenta |
3882 | | |
3883 | | , darkolivegreen |
3884 | | |
3885 | | , darkorange |
3886 | | |
3887 | | , darkorchid |
3888 | | |
3889 | | , darkred |
3890 | | |
3891 | | , darksalmon |
3892 | | |
3893 | | , darkseagreen |
3894 | | |
3895 | | , darkslateblue |
3896 | | |
3897 | | , darkslategray |
3898 | | |
3899 | | , darkslategrey |
3900 | | |
3901 | | , darkturquoise |
3902 | | |
3903 | | , darkviolet |
3904 | | |
3905 | | , deep pink |
3906 | | |
3907 | | , deep sky blue |
3908 | | |
3909 | | , deep skyblue |
3910 | | |
3911 | | , deeppink |
3912 | | |
3913 | | , deepskyblue |
3914 | | |
3915 | | , dim gray |
3916 | | |
3917 | | , dim grey |
3918 | | |
3919 | | , dimgray |
3920 | | |
3921 | | , dimgrey |
3922 | | |
3923 | | , dodger blue |
3924 | | |
3925 | | , dodgerblue |
3926 | | |
3927 | | , fire brick |
3928 | | |
3929 | | , firebrick |
3930 | | |
3931 | | , floral white |
3932 | | |
3933 | | , floralwhite |
3934 | | |
3935 | | , forest green |
3936 | | |
3937 | | , forestgreen |
3938 | | |
3939 | | , fuchsia |
3940 | | |
3941 | | , gainsboro |
3942 | | |
3943 | | , ghost white |
3944 | | |
3945 | | , ghostwhite |
3946 | | |
3947 | | , gold |
3948 | | |
3949 | | , goldenrod |
3950 | | |
3951 | | , gray |
3952 | | |
3953 | | , green |
3954 | | |
3955 | | , green yellow |
3956 | | |
3957 | | , greenyellow |
3958 | | |
3959 | | , grey |
3960 | | |
3961 | | , honeydew |
3962 | | |
3963 | | , hot pink |
3964 | | |
3965 | | , hotpink |
3966 | | |
3967 | | , indian red |
3968 | | |
3969 | | , indianred |
3970 | | |
3971 | | , indigo |
3972 | | |
3973 | | , ivory |
3974 | | |
3975 | | , khaki |
3976 | | |
3977 | | , lavender |
3978 | | |
3979 | | , lavender blush |
3980 | | |
3981 | | , lavenderblush |
3982 | | |
3983 | | , lawn green |
3984 | | |
3985 | | , lawngreen |
3986 | | |
3987 | | , lemon chiffon |
3988 | | |
3989 | | , lemonchiffon |
3990 | | |
3991 | | , light blue |
3992 | | |
3993 | | , light coral |
3994 | | |
3995 | | , light cyan |
3996 | | |
3997 | | , light goldenrod yellow |
3998 | | |
3999 | | , light gray |
4000 | | |
4001 | | , light green |
4002 | | |
4003 | | , light grey |
4004 | | |
4005 | | , light pink |
4006 | | |
4007 | | , light salmon |
4008 | | |
4009 | | , light sea green |
4010 | | |
4011 | | , light seagreen |
4012 | | |
4013 | | , light sky blue |
4014 | | |
4015 | | , light skyblue |
4016 | | |
4017 | | , light slate gray |
4018 | | |
4019 | | , light slate grey |
4020 | | |
4021 | | , light steel blue |
4022 | | |
4023 | | , light yellow |
4024 | | |
4025 | | , lightblue |
4026 | | |
4027 | | , lightcoral |
4028 | | |
4029 | | , lightcyan |
4030 | | |
4031 | | , lightgoldenrodyellow |
4032 | | |
4033 | | , lightgray |
4034 | | |
4035 | | , lightgreen |
4036 | | |
4037 | | , lightgrey |
4038 | | |
4039 | | , lightpink |
4040 | | |
4041 | | , lightsalmon |
4042 | | |
4043 | | , lightseagreen |
4044 | | |
4045 | | , lightskyblue |
4046 | | |
4047 | | , lightslategray |
4048 | | |
4049 | | , lightslategrey |
4050 | | |
4051 | | , lightsteelblue |
4052 | | |
4053 | | , lightyellow |
4054 | | |
4055 | | , lime |
4056 | | |
4057 | | , lime green |
4058 | | |
4059 | | , limegreen |
4060 | | |
4061 | | , linen |
4062 | | |
4063 | | , magenta |
4064 | | |
4065 | | , maroon |
4066 | | |
4067 | | , medium aquamarine |
4068 | | |
4069 | | , medium blue |
4070 | | |
4071 | | , medium orchid |
4072 | | |
4073 | | , medium purple |
4074 | | |
4075 | | , medium sea green |
4076 | | |
4077 | | , medium seagreen |
4078 | | |
4079 | | , medium slate blue |
4080 | | |
4081 | | , medium spring green |
4082 | | |
4083 | | , medium turquoise |
4084 | | |
4085 | | , medium violet red |
4086 | | |
4087 | | , mediumaquamarine |
4088 | | |
4089 | | , mediumblue |
4090 | | |
4091 | | , mediumorchid |
4092 | | |
4093 | | , mediumpurple |
4094 | | |
4095 | | , mediumseagreen |
4096 | | |
4097 | | , mediumslateblue |
4098 | | |
4099 | | , mediumspringgreen |
4100 | | |
4101 | | , mediumturquoise |
4102 | | |
4103 | | , mediumvioletred |
4104 | | |
4105 | | , midnight blue |
4106 | | |
4107 | | , midnightblue |
4108 | | |
4109 | | , mint cream |
4110 | | |
4111 | | , mintcream |
4112 | | |
4113 | | , misty rose |
4114 | | |
4115 | | , mistyrose |
4116 | | |
4117 | | , moccasin |
4118 | | |
4119 | | , navajo white |
4120 | | |
4121 | | , navajowhite |
4122 | | |
4123 | | , navy |
4124 | | |
4125 | | , old lace |
4126 | | |
4127 | | , oldlace |
4128 | | |
4129 | | , olive |
4130 | | |
4131 | | , olive drab |
4132 | | |
4133 | | , olivedrab |
4134 | | |
4135 | | , orange |
4136 | | |
4137 | | , orange red |
4138 | | |
4139 | | , orangered |
4140 | | |
4141 | | , orchid |
4142 | | |
4143 | | , pale goldenrod |
4144 | | |
4145 | | , pale green |
4146 | | |
4147 | | , pale turquoise |
4148 | | |
4149 | | , pale violet red |
4150 | | |
4151 | | , palegoldenrod |
4152 | | |
4153 | | , palegreen |
4154 | | |
4155 | | , paleturquoise |
4156 | | |
4157 | | , palevioletred |
4158 | | |
4159 | | , papaya whip |
4160 | | |
4161 | | , papayawhip |
4162 | | |
4163 | | , peach puff |
4164 | | |
4165 | | , peachpuff |
4166 | | |
4167 | | , peru |
4168 | | |
4169 | | , pink |
4170 | | |
4171 | | , plum |
4172 | | |
4173 | | , powder blue |
4174 | | |
4175 | | , powderblue |
4176 | | |
4177 | | , purple |
4178 | | |
4179 | | , rebecca purple |
4180 | | |
4181 | | , rebeccapurple |
4182 | | |
4183 | | , red |
4184 | | |
4185 | | , rosy brown |
4186 | | |
4187 | | , rosybrown |
4188 | | |
4189 | | , royal blue |
4190 | | |
4191 | | , royalblue |
4192 | | |
4193 | | , saddle brown |
4194 | | |
4195 | | , saddlebrown |
4196 | | |
4197 | | , salmon |
4198 | | |
4199 | | , sandy brown |
4200 | | |
4201 | | , sandybrown |
4202 | | |
4203 | | , sea green |
4204 | | |
4205 | | , seagreen |
4206 | | |
4207 | | , seashell |
4208 | | |
4209 | | , sienna |
4210 | | |
4211 | | , silver |
4212 | | |
4213 | | , sky blue |
4214 | | |
4215 | | , skyblue |
4216 | | |
4217 | | , slate blue |
4218 | | |
4219 | | , slate gray |
4220 | | |
4221 | | , slate grey |
4222 | | |
4223 | | , slateblue |
4224 | | |
4225 | | , slategray |
4226 | | |
4227 | | , slategrey |
4228 | | |
4229 | | , snow |
4230 | | |
4231 | | , spring green |
4232 | | |
4233 | | , springgreen |
4234 | | |
4235 | | , steel blue |
4236 | | |
4237 | | , steelblue |
4238 | | |
4239 | | , tan |
4240 | | |
4241 | | , teal |
4242 | | |
4243 | | , thistle |
4244 | | |
4245 | | , tomato |
4246 | | |
4247 | | , transparent |
4248 | | |
4249 | | , turquoise |
4250 | | |
4251 | | , violet |
4252 | | |
4253 | | , wheat |
4254 | | |
4255 | | , white |
4256 | | |
4257 | | , white smoke |
4258 | | |
4259 | | , whitesmoke |
4260 | | |
4261 | | , yellow |
4262 | | |
4263 | | , yellow green |
4264 | | |
4265 | | , and yellowgreen |
4266 | | |
4267 | | . |
4268 | | |
4269 | | > color sel navy |
4270 | | |
4271 | | > select subtract #34 |
4272 | | |
4273 | | Nothing selected |
4274 | | |
4275 | | > hide #34 models |
4276 | | |
4277 | | > show #35 models |
4278 | | |
4279 | | > select add #35 |
4280 | | |
4281 | | 7501 atoms, 7619 bonds, 953 residues, 1 model selected |
4282 | | |
4283 | | > color sel betav1 |
4284 | | |
4285 | | > select subtract #35 |
4286 | | |
4287 | | Nothing selected |
4288 | | |
4289 | | > hide #35 models |
4290 | | |
4291 | | > show #36 models |
4292 | | |
4293 | | > select add #36 |
4294 | | |
4295 | | 7214 atoms, 7373 bonds, 905 residues, 1 model selected |
4296 | | |
4297 | | > color sel betapv2 |
4298 | | |
4299 | | > color sel betapv3 |
4300 | | |
4301 | | > select subtract #36 |
4302 | | |
4303 | | Nothing selected |
4304 | | |
4305 | | > hide #36 models |
4306 | | |
4307 | | > show #37 models |
4308 | | |
4309 | | > select add #37 |
4310 | | |
4311 | | 4014 atoms, 4083 bonds, 511 residues, 1 model selected |
4312 | | |
4313 | | > color sel deltav3 |
4314 | | |
4315 | | > select subtract #37 |
4316 | | |
4317 | | Nothing selected |
4318 | | |
4319 | | > hide #37 models |
4320 | | |
4321 | | > show #38 models |
4322 | | |
4323 | | > select add #38 |
4324 | | |
4325 | | 6830 atoms, 6947 bonds, 874 residues, 1 model selected |
4326 | | |
4327 | | > close #39 |
4328 | | |
4329 | | > close #40 |
4330 | | |
4331 | | > open "/Users/becca/Desktop/Postdoc/COPI notebooks and resources/Structure |
4332 | | > files/alphafold/CopZ1_P61924.pdb" |
4333 | | |
4334 | | CopZ1_P61924.pdb title: |
4335 | | Alphafold monomer V2.0 prediction for coatomer subunit ζ-1 (P61924) [more |
4336 | | info...] |
4337 | | |
4338 | | Chain information for CopZ1_P61924.pdb #39 |
4339 | | --- |
4340 | | Chain | Description | UniProt |
4341 | | A | coatomer subunit ζ-1 | COPZ1_MOUSE 1-177 |
4342 | | |
4343 | | Computing secondary structure |
4344 | | |
4345 | | > hide #39 models |
4346 | | |
4347 | | > color sel gammav3 |
4348 | | |
4349 | | > select subtract #38 |
4350 | | |
4351 | | Nothing selected |
4352 | | |
4353 | | > hide #38 models |
4354 | | |
4355 | | > show #39 models |
4356 | | |
4357 | | > select add #39 |
4358 | | |
4359 | | 1420 atoms, 1441 bonds, 177 residues, 1 model selected |
4360 | | |
4361 | | > zetav3 |
4362 | | |
4363 | | Unknown command: zetav3 |
4364 | | |
4365 | | > color sel zetav3 |
4366 | | |
4367 | | > select subtract #39 |
4368 | | |
4369 | | Nothing selected |
4370 | | |
4371 | | > hide #39 models |
4372 | | |
4373 | | > show #34 models |
4374 | | |
4375 | | > select add #34 |
4376 | | |
4377 | | 9810 atoms, 10032 bonds, 1233 residues, 1 model selected |
4378 | | |
4379 | | > view matrix models #34,1,0,0,39.828,0,1,0,139.02,0,0,1,198.43 |
4380 | | |
4381 | | > ui mousemode right "rotate selected models" |
4382 | | |
4383 | | > view matrix models |
4384 | | > #34,0.99004,0.0096002,0.14042,38.534,-0.012281,0.99976,0.018237,138.66,-0.14022,-0.01978,0.98992,196.08 |
4385 | | |
4386 | | > view matrix models |
4387 | | > #34,0.13783,-0.38457,0.91275,20.074,-0.096945,0.91188,0.39884,134.94,-0.9857,-0.14346,0.088401,188.13 |
4388 | | |
4389 | | > view matrix models |
4390 | | > #34,-0.34037,-0.83282,0.43653,18.135,0.30375,0.34197,0.88926,142.89,-0.88988,0.43527,0.13658,185.25 |
4391 | | |
4392 | | > ui mousemode right "translate selected models" |
4393 | | |
4394 | | > view matrix models |
4395 | | > #34,-0.34037,-0.83282,0.43653,139.16,0.30375,0.34197,0.88926,104.42,-0.88988,0.43527,0.13658,153.4 |
4396 | | |
4397 | | > view matrix models |
4398 | | > #34,-0.34037,-0.83282,0.43653,161.88,0.30375,0.34197,0.88926,148.44,-0.88988,0.43527,0.13658,135.72 |
4399 | | |
4400 | | > ui mousemode right "rotate selected models" |
4401 | | |
4402 | | > view matrix models |
4403 | | > #34,-0.64868,-0.43267,-0.62611,161.12,0.47729,-0.87206,0.10814,166.41,-0.59279,-0.22869,0.7722,141.4 |
4404 | | |
4405 | | > ui mousemode right "translate selected models" |
4406 | | |
4407 | | > view matrix models |
4408 | | > #34,-0.64868,-0.43267,-0.62611,149.78,0.47729,-0.87206,0.10814,138.8,-0.59279,-0.22869,0.7722,155.26 |
4409 | | |
4410 | | > fitmap #34 inMap #1 |
4411 | | |
4412 | | Fit molecule CopA-F8WHL2.pdb (#34) to map |
4413 | | W20_J3623_consensus_structure_sharp.mrc (#1) using 9810 atoms |
4414 | | average map value = 0.1956, steps = 172 |
4415 | | shifted from previous position = 12.4 |
4416 | | rotated from previous position = 16.7 degrees |
4417 | | atoms outside contour = 6664, contour level = 0.26383 |
4418 | | |
4419 | | Position of CopA-F8WHL2.pdb (#34) relative to |
4420 | | W20_J3623_consensus_structure_sharp.mrc (#1) coordinates: |
4421 | | Matrix rotation and translation |
4422 | | -0.40275740 -0.50265546 -0.76493398 155.53344658 |
4423 | | 0.55537393 -0.79849851 0.23229283 137.87047167 |
4424 | | -0.72756189 -0.33126673 0.60076291 143.99545516 |
4425 | | Axis -0.46989046 -0.03116049 0.88217458 |
4426 | | Axis point 89.79461102 105.24732460 0.00000000 |
4427 | | Rotation angle (degrees) 143.15364698 |
4428 | | Shift along axis 49.64933605 |
4429 | | |
4430 | | |
4431 | | > view matrix models |
4432 | | > #34,-0.40276,-0.50266,-0.76493,152.08,0.55537,-0.7985,0.23229,129.79,-0.72756,-0.33127,0.60076,131.15 |
4433 | | |
4434 | | > fitmap #34 inMap #1 |
4435 | | |
4436 | | Fit molecule CopA-F8WHL2.pdb (#34) to map |
4437 | | W20_J3623_consensus_structure_sharp.mrc (#1) using 9810 atoms |
4438 | | average map value = 0.3026, steps = 204 |
4439 | | shifted from previous position = 8.55 |
4440 | | rotated from previous position = 12.6 degrees |
4441 | | atoms outside contour = 4835, contour level = 0.26383 |
4442 | | |
4443 | | Position of CopA-F8WHL2.pdb (#34) relative to |
4444 | | W20_J3623_consensus_structure_sharp.mrc (#1) coordinates: |
4445 | | Matrix rotation and translation |
4446 | | -0.54728967 -0.56189790 -0.62027800 158.57511981 |
4447 | | 0.58976792 -0.78477262 0.19054069 127.65605082 |
4448 | | -0.59384159 -0.26153911 0.76088730 134.18075471 |
4449 | | Axis -0.36531666 -0.02136273 0.93063815 |
4450 | | Axis point 81.60160811 99.25852793 0.00000000 |
4451 | | Rotation angle (degrees) 141.77504660 |
4452 | | Shift along axis 64.21651520 |
4453 | | |
4454 | | |
4455 | | > select subtract #34 |
4456 | | |
4457 | | Nothing selected |
4458 | | |
4459 | | > hide #34 models |
4460 | | |
4461 | | > show #35 models |
4462 | | |
4463 | | > select add #35 |
4464 | | |
4465 | | 7501 atoms, 7619 bonds, 953 residues, 1 model selected |
4466 | | |
4467 | | > view matrix models #35,1,0,0,116.88,0,1,0,-7.2403,0,0,1,111.96 |
4468 | | |
4469 | | > view matrix models #35,1,0,0,112.47,0,1,0,89.143,0,0,1,196.06 |
4470 | | |
4471 | | > ui mousemode right "rotate selected models" |
4472 | | |
4473 | | > view matrix models |
4474 | | > #35,0.0065824,0.90646,-0.42225,117.47,0.83324,-0.23843,-0.49886,91.055,-0.55287,-0.34855,-0.75686,209.75 |
4475 | | |
4476 | | > ui mousemode right "translate selected models" |
4477 | | |
4478 | | > view matrix models |
4479 | | > #35,0.0065824,0.90646,-0.42225,96.987,0.83324,-0.23843,-0.49886,131.53,-0.55287,-0.34855,-0.75686,188.84 |
4480 | | |
4481 | | > view matrix models |
4482 | | > #35,0.0065824,0.90646,-0.42225,132.29,0.83324,-0.23843,-0.49886,123.6,-0.55287,-0.34855,-0.75686,174.35 |
4483 | | |
4484 | | > view matrix models |
4485 | | > #35,0.0065824,0.90646,-0.42225,129.73,0.83324,-0.23843,-0.49886,153.17,-0.55287,-0.34855,-0.75686,162.06 |
4486 | | |
4487 | | > fitmap #35 inMap #1 |
4488 | | |
4489 | | Fit molecule CopB_ Q9JIF7.pdb (#35) to map |
4490 | | W20_J3623_consensus_structure_sharp.mrc (#1) using 7501 atoms |
4491 | | average map value = 0.272, steps = 140 |
4492 | | shifted from previous position = 13 |
4493 | | rotated from previous position = 19.4 degrees |
4494 | | atoms outside contour = 4336, contour level = 0.26383 |
4495 | | |
4496 | | Position of CopB_ Q9JIF7.pdb (#35) relative to |
4497 | | W20_J3623_consensus_structure_sharp.mrc (#1) coordinates: |
4498 | | Matrix rotation and translation |
4499 | | 0.29878946 0.85180997 -0.43028434 135.66407916 |
4500 | | 0.69839777 -0.50244520 -0.50969537 144.97471611 |
4501 | | -0.65035790 -0.14821802 -0.74502752 155.00132184 |
4502 | | Axis 0.80301824 0.48889117 -0.34080365 |
4503 | | Axis point 0.00000000 17.11569470 112.17244297 |
4504 | | Rotation angle (degrees) 166.99275965 |
4505 | | Shift along axis 126.99257230 |
4506 | | |
4507 | | |
4508 | | > select subtract #35 |
4509 | | |
4510 | | Nothing selected |
4511 | | |
4512 | | > hide #35 models |
4513 | | |
4514 | | > show #36 models |
4515 | | |
4516 | | > select add #36 |
4517 | | |
4518 | | 7214 atoms, 7373 bonds, 905 residues, 1 model selected |
4519 | | |
4520 | | > view matrix models #36,1,0,0,158.6,0,1,0,131.6,0,0,1,186.36 |
4521 | | |
4522 | | > view matrix models #36,1,0,0,112.24,0,1,0,235.54,0,0,1,195.51 |
4523 | | |
4524 | | > ui mousemode right "rotate selected models" |
4525 | | |
4526 | | > view matrix models |
4527 | | > #36,0.60674,-0.015574,0.79475,88.102,0.72366,0.42451,-0.54415,257.75,-0.3289,0.90529,0.26884,209.07 |
4528 | | |
4529 | | > ui mousemode right "translate selected models" |
4530 | | |
4531 | | > view matrix models |
4532 | | > #36,0.60674,-0.015574,0.79475,110.55,0.72366,0.42451,-0.54415,182.27,-0.3289,0.90529,0.26884,183.11 |
4533 | | |
4534 | | > view matrix models |
4535 | | > #36,0.60674,-0.015574,0.79475,107.23,0.72366,0.42451,-0.54415,189.41,-0.3289,0.90529,0.26884,154.84 |
4536 | | |
4537 | | > view matrix models |
4538 | | > #36,0.60674,-0.015574,0.79475,127.45,0.72366,0.42451,-0.54415,196.73,-0.3289,0.90529,0.26884,180.58 |
4539 | | |
4540 | | > fitmap #36 inMap #1 |
4541 | | |
4542 | | Fit molecule CopBprime_O55029.pdb (#36) to map |
4543 | | W20_J3623_consensus_structure_sharp.mrc (#1) using 7214 atoms |
4544 | | average map value = 0.2113, steps = 200 |
4545 | | shifted from previous position = 4.95 |
4546 | | rotated from previous position = 18 degrees |
4547 | | atoms outside contour = 5449, contour level = 0.26383 |
4548 | | |
4549 | | Position of CopBprime_O55029.pdb (#36) relative to |
4550 | | W20_J3623_consensus_structure_sharp.mrc (#1) coordinates: |
4551 | | Matrix rotation and translation |
4552 | | 0.50276875 0.12737660 0.85498467 126.31561890 |
4553 | | 0.61912593 0.63718187 -0.45900148 191.39912628 |
4554 | | -0.60324678 0.76011477 0.24149298 177.83802946 |
4555 | | Axis 0.62095632 0.74274954 0.25047230 |
4556 | | Axis point 74.13696267 0.00000000 87.94186273 |
4557 | | Rotation angle (degrees) 79.00508960 |
4558 | | Shift along axis 265.14159471 |
4559 | | |
4560 | | |
4561 | | > select subtract #36 |
4562 | | |
4563 | | Nothing selected |
4564 | | |
4565 | | > hide #36 models |
4566 | | |
4567 | | > show #37 models |
4568 | | |
4569 | | > select add #37 |
4570 | | |
4571 | | 4014 atoms, 4083 bonds, 511 residues, 1 model selected |
4572 | | |
4573 | | > view matrix models #37,1,0,0,30.593,0,1,0,199.33,0,0,1,198.85 |
4574 | | |
4575 | | > view matrix models #37,1,0,0,157.99,0,1,0,204.81,0,0,1,141.3 |
4576 | | |
4577 | | > ui mousemode right "rotate selected models" |
4578 | | |
4579 | | > view matrix models |
4580 | | > #37,0.43622,0.67922,-0.59023,141.13,-0.78114,-0.039782,-0.62309,191.16,-0.44669,0.73286,0.51321,126.99 |
4581 | | |
4582 | | > ui mousemode right "translate selected models" |
4583 | | |
4584 | | > view matrix models |
4585 | | > #37,0.43622,0.67922,-0.59023,150.33,-0.78114,-0.039782,-0.62309,186.91,-0.44669,0.73286,0.51321,121.12 |
4586 | | |
4587 | | > view matrix models |
4588 | | > #37,0.43622,0.67922,-0.59023,147.14,-0.78114,-0.039782,-0.62309,187.14,-0.44669,0.73286,0.51321,128.48 |
4589 | | |
4590 | | > fitmap #37 inMap #1 |
4591 | | |
4592 | | Fit molecule CopD_full_Q5XJY5.pdb (#37) to map |
4593 | | W20_J3623_consensus_structure_sharp.mrc (#1) using 4014 atoms |
4594 | | average map value = 0.1388, steps = 96 |
4595 | | shifted from previous position = 7.92 |
4596 | | rotated from previous position = 10.6 degrees |
4597 | | atoms outside contour = 3125, contour level = 0.26383 |
4598 | | |
4599 | | Position of CopD_full_Q5XJY5.pdb (#37) relative to |
4600 | | W20_J3623_consensus_structure_sharp.mrc (#1) coordinates: |
4601 | | Matrix rotation and translation |
4602 | | 0.41505472 0.79215751 -0.44745509 152.46783193 |
4603 | | -0.75869096 0.02993242 -0.65076269 180.94994695 |
4604 | | -0.50211314 0.60958226 0.61342633 132.05032423 |
4605 | | Axis 0.63044142 0.02734069 -0.77575518 |
4606 | | Axis point 250.00196774 -8.95488713 0.00000000 |
4607 | | Rotation angle (degrees) 88.32633929 |
4608 | | Shift along axis -1.36938916 |
4609 | | |
4610 | | |
4611 | | > view matrix models |
4612 | | > #37,0.41505,0.79216,-0.44746,143.76,-0.75869,0.029932,-0.65076,186.11,-0.50211,0.60958,0.61343,122.85 |
4613 | | |
4614 | | > view matrix models |
4615 | | > #37,0.41505,0.79216,-0.44746,143.43,-0.75869,0.029932,-0.65076,185.75,-0.50211,0.60958,0.61343,122.85 |
4616 | | |
4617 | | > view matrix models |
4618 | | > #37,0.41505,0.79216,-0.44746,142.4,-0.75869,0.029932,-0.65076,174.97,-0.50211,0.60958,0.61343,117.62 |
4619 | | |
4620 | | > fitmap #37 inMap #1 |
4621 | | |
4622 | | Fit molecule CopD_full_Q5XJY5.pdb (#37) to map |
4623 | | W20_J3623_consensus_structure_sharp.mrc (#1) using 4014 atoms |
4624 | | average map value = 0.1655, steps = 240 |
4625 | | shifted from previous position = 19.2 |
4626 | | rotated from previous position = 30 degrees |
4627 | | atoms outside contour = 2918, contour level = 0.26383 |
4628 | | |
4629 | | Position of CopD_full_Q5XJY5.pdb (#37) relative to |
4630 | | W20_J3623_consensus_structure_sharp.mrc (#1) coordinates: |
4631 | | Matrix rotation and translation |
4632 | | 0.54325309 0.50781797 -0.66857834 156.40844261 |
4633 | | -0.83919938 0.30481815 -0.45036685 164.83901629 |
4634 | | -0.02490957 0.80573371 0.59175392 110.35264351 |
4635 | | Axis 0.64381104 -0.32991074 -0.69041020 |
4636 | | Axis point 270.01565892 -103.01400891 0.00000000 |
4637 | | Rotation angle (degrees) 77.29610173 |
4638 | | Shift along axis -29.87327013 |
4639 | | |
4640 | | |
4641 | | > view matrix models |
4642 | | > #37,0.54325,0.50782,-0.66858,154.11,-0.8392,0.30482,-0.45037,160.13,-0.02491,0.80573,0.59175,113.09 |
4643 | | |
4644 | | > view matrix models |
4645 | | > #37,0.54325,0.50782,-0.66858,152.8,-0.8392,0.30482,-0.45037,160.99,-0.02491,0.80573,0.59175,116.7 |
4646 | | |
4647 | | > fitmap #37 inMap #1 |
4648 | | |
4649 | | Fit molecule CopD_full_Q5XJY5.pdb (#37) to map |
4650 | | W20_J3623_consensus_structure_sharp.mrc (#1) using 4014 atoms |
4651 | | average map value = 0.1527, steps = 200 |
4652 | | shifted from previous position = 8.47 |
4653 | | rotated from previous position = 9.78 degrees |
4654 | | atoms outside contour = 3000, contour level = 0.26383 |
4655 | | |
4656 | | Position of CopD_full_Q5XJY5.pdb (#37) relative to |
4657 | | W20_J3623_consensus_structure_sharp.mrc (#1) coordinates: |
4658 | | Matrix rotation and translation |
4659 | | 0.52381084 0.45458159 -0.72040113 159.66823341 |
4660 | | -0.84399300 0.16247112 -0.51115453 164.46346911 |
4661 | | -0.11531706 0.87576179 0.46876781 118.49109089 |
4662 | | Axis 0.69555148 -0.30345531 -0.65124728 |
4663 | | Axis point 0.00000000 33.10752297 257.99354223 |
4664 | | Rotation angle (degrees) 85.55368957 |
4665 | | Shift along axis -16.01683616 |
4666 | | |
4667 | | |
4668 | | > select subtract #37 |
4669 | | |
4670 | | Nothing selected |
4671 | | |
4672 | | > hide #37 models |
4673 | | |
4674 | | > show #38 models |
4675 | | |
4676 | | > select add #38 |
4677 | | |
4678 | | 6830 atoms, 6947 bonds, 874 residues, 1 model selected |
4679 | | |
4680 | | > view matrix models #38,1,0,0,4.9195,0,1,0,158.21,0,0,1,93.306 |
4681 | | |
4682 | | > view matrix models #38,1,0,0,20.598,0,1,0,103.1,0,0,1,122.07 |
4683 | | |
4684 | | > view matrix models #38,1,0,0,72.888,0,1,0,68.344,0,0,1,130.87 |
4685 | | |
4686 | | > view matrix models #38,1,0,0,89.688,0,1,0,109.93,0,0,1,127.2 |
4687 | | |
4688 | | > ui mousemode right "rotate selected models" |
4689 | | |
4690 | | > view matrix models |
4691 | | > #38,-0.10567,0.8283,0.55022,86.046,-0.72387,0.3153,-0.61367,108.49,-0.68179,-0.46314,0.56627,125.7 |
4692 | | |
4693 | | > ui mousemode right "translate selected models" |
4694 | | |
4695 | | > view matrix models |
4696 | | > #38,-0.10567,0.8283,0.55022,94.304,-0.72387,0.3153,-0.61367,138.65,-0.68179,-0.46314,0.56627,138.14 |
4697 | | |
4698 | | > view matrix models |
4699 | | > #38,-0.10567,0.8283,0.55022,112.86,-0.72387,0.3153,-0.61367,126.46,-0.68179,-0.46314,0.56627,130.13 |
4700 | | |
4701 | | > fitmap #38 inMap #1 |
4702 | | |
4703 | | Fit molecule CopG1_ Q9QZE5.pdb (#38) to map |
4704 | | W20_J3623_consensus_structure_sharp.mrc (#1) using 6830 atoms |
4705 | | average map value = 0.2013, steps = 104 |
4706 | | shifted from previous position = 5.21 |
4707 | | rotated from previous position = 10.3 degrees |
4708 | | atoms outside contour = 4944, contour level = 0.26383 |
4709 | | |
4710 | | Position of CopG1_ Q9QZE5.pdb (#38) relative to |
4711 | | W20_J3623_consensus_structure_sharp.mrc (#1) coordinates: |
4712 | | Matrix rotation and translation |
4713 | | -0.18152050 0.89700589 0.40302697 113.56564500 |
4714 | | -0.70329404 0.16803702 -0.69075398 122.94853762 |
4715 | | -0.68733384 -0.40883247 0.60035673 126.40848670 |
4716 | | Axis 0.14406783 0.55719735 -0.81778700 |
4717 | | Axis point 133.98876902 46.90995141 0.00000000 |
4718 | | Rotation angle (degrees) 101.92103286 |
4719 | | Shift along axis -18.50746083 |
4720 | | |
4721 | | |
4722 | | > select subtract #38 |
4723 | | |
4724 | | Nothing selected |
4725 | | |
4726 | | > hide #38 models |
4727 | | |
4728 | | > show #39 models |
4729 | | |
4730 | | > select add #39 |
4731 | | |
4732 | | 1420 atoms, 1441 bonds, 177 residues, 1 model selected |
4733 | | |
4734 | | > view matrix models #39,1,0,0,9.0898,0,1,0,160.45,0,0,1,-9.3444 |
4735 | | |
4736 | | > view matrix models #39,1,0,0,60.276,0,1,0,127.59,0,0,1,121.27 |
4737 | | |
4738 | | > view matrix models #39,1,0,0,127.7,0,1,0,116.45,0,0,1,94.356 |
4739 | | |
4740 | | > view matrix models #39,1,0,0,126.09,0,1,0,140.04,0,0,1,103.22 |
4741 | | |
4742 | | > ui mousemode right "rotate selected models" |
4743 | | |
4744 | | > view matrix models |
4745 | | > #39,0.77404,0.11317,-0.62294,121.61,-0.58601,0.50055,-0.63722,130.26,0.2397,0.85828,0.45376,105.69 |
4746 | | |
4747 | | > fitmap #39 inMap #1 |
4748 | | |
4749 | | Fit molecule CopZ1_P61924.pdb (#39) to map |
4750 | | W20_J3623_consensus_structure_sharp.mrc (#1) using 1420 atoms |
4751 | | average map value = 0.2019, steps = 236 |
4752 | | shifted from previous position = 4.91 |
4753 | | rotated from previous position = 38.4 degrees |
4754 | | atoms outside contour = 1084, contour level = 0.26383 |
4755 | | |
4756 | | Position of CopZ1_P61924.pdb (#39) relative to |
4757 | | W20_J3623_consensus_structure_sharp.mrc (#1) coordinates: |
4758 | | Matrix rotation and translation |
4759 | | 0.67082708 0.40413817 -0.62182263 120.91871935 |
4760 | | -0.65153203 -0.07936382 -0.75445836 130.14553587 |
4761 | | -0.35425563 0.91124845 0.21006956 98.85805425 |
4762 | | Axis 0.83698462 -0.13444710 -0.53045332 |
4763 | | Axis point 0.00000000 12.35504647 160.39862757 |
4764 | | Rotation angle (degrees) 95.69503877 |
4765 | | Shift along axis 31.26983397 |
4766 | | |
4767 | | |
4768 | | > select subtract #39 |
4769 | | |
4770 | | Nothing selected |
4771 | | |
4772 | | > hide #39 models |
4773 | | |
4774 | | > show #39 models |
4775 | | |
4776 | | > show #38 models |
4777 | | |
4778 | | > show #37 models |
4779 | | |
4780 | | > show #36 models |
4781 | | |
4782 | | > show #35 models |
4783 | | |
4784 | | > show #34 models |
4785 | | |
4786 | | > hide #!1 models |
4787 | | |
4788 | | > show #!3 models |
4789 | | |
4790 | | > fitmap #36 inMap #3 |
4791 | | |
4792 | | Fit molecule CopBprime_O55029.pdb (#36) to map |
4793 | | W20_J3985_004_volume_map_csparc_refine_of_relion_job121_bprime_mask.mrc (#3) |
4794 | | using 7214 atoms |
4795 | | average map value = 0.2322, steps = 64 |
4796 | | shifted from previous position = 2.88 |
4797 | | rotated from previous position = 4.24 degrees |
4798 | | atoms outside contour = 4591, contour level = 0.26875 |
4799 | | |
4800 | | Position of CopBprime_O55029.pdb (#36) relative to |
4801 | | W20_J3985_004_volume_map_csparc_refine_of_relion_job121_bprime_mask.mrc (#3) |
4802 | | coordinates: |
4803 | | Matrix rotation and translation |
4804 | | -0.39263805 -0.91469425 -0.09575906 92.65990042 |
4805 | | -0.55910845 0.32006970 -0.76482228 179.90077864 |
4806 | | 0.73022811 -0.24675864 -0.63708483 150.47739175 |
4807 | | Axis 0.49918086 -0.79588095 0.34262513 |
4808 | | Axis point 73.56777391 0.00000000 134.22819381 |
4809 | | Rotation angle (degrees) 148.74058258 |
4810 | | Shift along axis -45.36821860 |
4811 | | |
4812 | | |
4813 | | > fitmap #35 inMap #3 |
4814 | | |
4815 | | Fit molecule CopB_ Q9JIF7.pdb (#35) to map |
4816 | | W20_J3985_004_volume_map_csparc_refine_of_relion_job121_bprime_mask.mrc (#3) |
4817 | | using 7501 atoms |
4818 | | average map value = 0.2689, steps = 112 |
4819 | | shifted from previous position = 7.58 |
4820 | | rotated from previous position = 7.06 degrees |
4821 | | atoms outside contour = 3837, contour level = 0.26875 |
4822 | | |
4823 | | Position of CopB_ Q9JIF7.pdb (#35) relative to |
4824 | | W20_J3985_004_volume_map_csparc_refine_of_relion_job121_bprime_mask.mrc (#3) |
4825 | | coordinates: |
4826 | | Matrix rotation and translation |
4827 | | -0.23385104 0.28135848 0.93067238 134.35318178 |
4828 | | -0.41876188 -0.89302545 0.16475441 150.63397364 |
4829 | | 0.87746918 -0.35120213 0.32665714 138.57787474 |
4830 | | Axis -0.59215049 0.06105999 -0.80351072 |
4831 | | Axis point 39.18867412 76.76513978 0.00000000 |
4832 | | Rotation angle (degrees) 154.17248796 |
4833 | | Shift along axis -181.70840106 |
4834 | | |
4835 | | |
4836 | | > fitmap #34 inMap #3 |
4837 | | |
4838 | | Fit molecule CopA-F8WHL2.pdb (#34) to map |
4839 | | W20_J3985_004_volume_map_csparc_refine_of_relion_job121_bprime_mask.mrc (#3) |
4840 | | using 9810 atoms |
4841 | | average map value = 0.2333, steps = 88 |
4842 | | shifted from previous position = 0.574 |
4843 | | rotated from previous position = 1.73 degrees |
4844 | | atoms outside contour = 5294, contour level = 0.26875 |
4845 | | |
4846 | | Position of CopA-F8WHL2.pdb (#34) relative to |
4847 | | W20_J3985_004_volume_map_csparc_refine_of_relion_job121_bprime_mask.mrc (#3) |
4848 | | coordinates: |
4849 | | Matrix rotation and translation |
4850 | | 0.01921606 0.93572095 -0.35221737 156.63147150 |
4851 | | 0.36634751 0.32119202 0.87328416 124.11551889 |
4852 | | 0.93027967 -0.14581504 -0.33662699 137.77469414 |
4853 | | Axis -0.58763841 -0.73952029 -0.32831515 |
4854 | | Axis point 4.51722837 0.00000000 49.95590579 |
4855 | | Rotation angle (degrees) 119.87500102 |
4856 | | Shift along axis -229.06213308 |
4857 | | |
4858 | | |
4859 | | > hide #!3 models |
4860 | | |
4861 | | > show #!4 models |
4862 | | |
4863 | | > hide #!4 models |
4864 | | |
4865 | | > show #!5 models |
4866 | | |
4867 | | > hide #!5 models |
4868 | | |
4869 | | > show #!6 models |
4870 | | |
4871 | | > hide #!6 models |
4872 | | |
4873 | | > show #!7 models |
4874 | | |
4875 | | > hide #!7 models |
4876 | | |
4877 | | > show #!8 models |
4878 | | |
4879 | | > hide #!8 models |
4880 | | |
4881 | | > show #!9 models |
4882 | | |
4883 | | > fitmap #38 inMap #9 |
4884 | | |
4885 | | Fit molecule CopG1_ Q9QZE5.pdb (#38) to map |
4886 | | W20_J3988_004_volume_map_csparc_refine_of_relion_job126_gammazeta_mask.mrc |
4887 | | (#9) using 6830 atoms |
4888 | | average map value = 0.2056, steps = 112 |
4889 | | shifted from previous position = 3.87 |
4890 | | rotated from previous position = 8.6 degrees |
4891 | | atoms outside contour = 4655, contour level = 0.28232 |
4892 | | |
4893 | | Position of CopG1_ Q9QZE5.pdb (#38) relative to |
4894 | | W20_J3988_004_volume_map_csparc_refine_of_relion_job126_gammazeta_mask.mrc |
4895 | | (#9) coordinates: |
4896 | | Matrix rotation and translation |
4897 | | 0.97147165 -0.22568138 0.07287489 181.98815327 |
4898 | | -0.21631778 -0.96919397 -0.11776953 160.81365369 |
4899 | | 0.09720830 0.09864563 -0.99036336 147.52870008 |
4900 | | Axis 0.99282083 -0.11163133 0.04295621 |
4901 | | Axis point 0.00000000 86.77702657 74.77347983 |
4902 | | Rotation angle (degrees) 173.74290119 |
4903 | | Shift along axis 169.06706116 |
4904 | | |
4905 | | |
4906 | | > hide #!9 models |
4907 | | |
4908 | | > show #!10 models |
4909 | | |
4910 | | > hide #!10 models |
4911 | | |
4912 | | > show #!10 models |
4913 | | |
4914 | | > hide #!10 models |
4915 | | |
4916 | | > show #!10 models |
4917 | | |
4918 | | > hide #!10 models |
4919 | | |
4920 | | > show #!11 models |
4921 | | |
4922 | | > hide #!11 models |
4923 | | |
4924 | | > show #!23 models |
4925 | | |
4926 | | > fitmap #38 inMap #23 |
4927 | | |
4928 | | Fit molecule CopG1_ Q9QZE5.pdb (#38) to map |
4929 | | W20_J4026_004_volume_map_gammazeta_local.mrc (#23) using 6830 atoms |
4930 | | average map value = 0.1938, steps = 88 |
4931 | | shifted from previous position = 1.48 |
4932 | | rotated from previous position = 1.93 degrees |
4933 | | atoms outside contour = 4539, contour level = 0.26014 |
4934 | | |
4935 | | Position of CopG1_ Q9QZE5.pdb (#38) relative to |
4936 | | W20_J4026_004_volume_map_gammazeta_local.mrc (#23) coordinates: |
4937 | | Matrix rotation and translation |
4938 | | -0.15863519 0.95166183 0.26301070 116.52908933 |
4939 | | -0.70798986 0.07602756 -0.70211834 119.07331926 |
4940 | | -0.68817529 -0.29758959 0.66170629 125.26733072 |
4941 | | Axis 0.20689796 0.48648815 -0.84883597 |
4942 | | Axis point 135.80061352 37.60568602 0.00000000 |
4943 | | Rotation angle (degrees) 102.14876416 |
4944 | | Shift along axis -24.29402645 |
4945 | | |
4946 | | |
4947 | | > hide #!23 models |
4948 | | |
4949 | | > show #!25 models |
4950 | | |
4951 | | > fitmap #38 inMap #23 |
4952 | | |
4953 | | Fit molecule CopG1_ Q9QZE5.pdb (#38) to map |
4954 | | W20_J4026_004_volume_map_gammazeta_local.mrc (#23) using 6830 atoms |
4955 | | average map value = 0.1938, steps = 48 |
4956 | | shifted from previous position = 0.0265 |
4957 | | rotated from previous position = 0.0358 degrees |
4958 | | atoms outside contour = 4543, contour level = 0.26014 |
4959 | | |
4960 | | Position of CopG1_ Q9QZE5.pdb (#38) relative to |
4961 | | W20_J4026_004_volume_map_gammazeta_local.mrc (#23) coordinates: |
4962 | | Matrix rotation and translation |
4963 | | -0.15917554 0.95152929 0.26316374 116.52898909 |
4964 | | -0.70811196 0.07570421 -0.70203014 119.09089645 |
4965 | | -0.68792485 -0.29809542 0.66173902 125.28763210 |
4966 | | Axis 0.20661307 0.48648289 -0.84890838 |
4967 | | Axis point 135.76856788 37.64517816 0.00000000 |
4968 | | Rotation angle (degrees) 102.17311623 |
4969 | | Shift along axis -24.34562541 |
4970 | | |
4971 | | |
4972 | | > fitmap #38 inMap #25 |
4973 | | |
4974 | | Fit molecule CopG1_ Q9QZE5.pdb (#38) to map |
4975 | | W20_J4027_005_volume_map_gammazeta_local.mrc (#25) using 6830 atoms |
4976 | | average map value = 0.2208, steps = 312 |
4977 | | shifted from previous position = 9.89 |
4978 | | rotated from previous position = 43.3 degrees |
4979 | | atoms outside contour = 4003, contour level = 0.2532 |
4980 | | |
4981 | | Position of CopG1_ Q9QZE5.pdb (#38) relative to |
4982 | | W20_J4027_005_volume_map_gammazeta_local.mrc (#25) coordinates: |
4983 | | Matrix rotation and translation |
4984 | | -0.51349164 0.78944189 0.33631509 107.60498851 |
4985 | | -0.18358145 0.28179025 -0.94174949 122.78040261 |
4986 | | -0.83822681 -0.54532170 0.00022995 120.22835474 |
4987 | | Axis 0.25155556 0.74531240 -0.61743762 |
4988 | | Axis point 81.37402006 0.00000000 79.41692880 |
4989 | | Rotation angle (degrees) 128.00539846 |
4990 | | Shift along axis 44.34488087 |
4991 | | |
4992 | | |
4993 | | > hide #!25 models |
4994 | | |
4995 | | > show #!25 models |
4996 | | |
4997 | | > color #25 #929292ff models |
4998 | | |
4999 | | > color #25 #92929297 models |
5000 | | |
5001 | | > select add #39 |
5002 | | |
5003 | | 1420 atoms, 1441 bonds, 177 residues, 1 model selected |
5004 | | |
5005 | | > view matrix models |
5006 | | > #39,0.049821,0.95099,-0.30519,123.19,-0.98555,-0.0027194,-0.16936,132.83,-0.16189,0.30922,0.93711,101.07 |
5007 | | |
5008 | | > ui mousemode right "translate selected models" |
5009 | | |
5010 | | > view matrix models |
5011 | | > #39,0.049821,0.95099,-0.30519,120.81,-0.98555,-0.0027194,-0.16936,149.13,-0.16189,0.30922,0.93711,88.072 |
5012 | | |
5013 | | > view matrix models |
5014 | | > #39,0.049821,0.95099,-0.30519,127.16,-0.98555,-0.0027194,-0.16936,146.48,-0.16189,0.30922,0.93711,93.373 |
5015 | | |
5016 | | > fitmap #39 inMap #25 |
5017 | | |
5018 | | Fit molecule CopZ1_P61924.pdb (#39) to map |
5019 | | W20_J4027_005_volume_map_gammazeta_local.mrc (#25) using 1420 atoms |
5020 | | average map value = 0.2155, steps = 132 |
5021 | | shifted from previous position = 12.7 |
5022 | | rotated from previous position = 24.5 degrees |
5023 | | atoms outside contour = 816, contour level = 0.2532 |
5024 | | |
5025 | | Position of CopZ1_P61924.pdb (#39) relative to |
5026 | | W20_J4027_005_volume_map_gammazeta_local.mrc (#25) coordinates: |
5027 | | Matrix rotation and translation |
5028 | | -0.02172282 0.87569029 -0.48238434 117.17569269 |
5029 | | -0.85704432 -0.26475204 -0.44201968 143.50099331 |
5030 | | -0.51478457 0.40382284 0.75625654 98.49893470 |
5031 | | Axis 0.43861563 0.01680130 -0.89851770 |
5032 | | Axis point 143.73837501 16.52006781 0.00000000 |
5033 | | Rotation angle (degrees) 105.37343878 |
5034 | | Shift along axis -34.69694324 |
5035 | | |
5036 | | |
5037 | | > select subtract #39 |
5038 | | |
5039 | | Nothing selected |
5040 | | |
5041 | | > hide #39 models |
5042 | | |
5043 | | > hide #38 models |
5044 | | |
5045 | | > hide #37 models |
5046 | | |
5047 | | > hide #36 models |
5048 | | |
5049 | | > hide #35 models |
5050 | | |
5051 | | > show #35 models |
5052 | | |
5053 | | > hide #35 models |
5054 | | |
5055 | | > hide #34 models |
5056 | | |
5057 | | > show #34 models |
5058 | | |
5059 | | > show #35 models |
5060 | | |
5061 | | > show #36 models |
5062 | | |
5063 | | > show #37 models |
5064 | | |
5065 | | > show #38 models |
5066 | | |
5067 | | > show #39 models |
5068 | | |
5069 | | > open "/Users/becca/Desktop/Postdoc/COPI notebooks and resources/Alphafold |
5070 | | > predictions/fold_2025_01_17_alpha1_330gamma1_330zeta/fold_2025_01_17_alpha1_330gamma1_330zeta_model_0.cif" |
5071 | | |
5072 | | Chain information for fold_2025_01_17_alpha1_330gamma1_330zeta_model_0.cif #40 |
5073 | | --- |
5074 | | Chain | Description |
5075 | | A | . |
5076 | | B | . |
5077 | | C | . |
5078 | | |
5079 | | Computing secondary structure |
5080 | | |
5081 | | > select add #40 |
5082 | | |
5083 | | 6676 atoms, 6817 bonds, 837 residues, 1 model selected |
5084 | | |
5085 | | > ui tool show Matchmaker |
5086 | | |
5087 | | > matchmaker #40 to #34 |
5088 | | |
5089 | | Computing secondary structure |
5090 | | [Repeated 1 time(s)] Parameters |
5091 | | --- |
5092 | | Chain pairing | bb |
5093 | | Alignment algorithm | Needleman-Wunsch |
5094 | | Similarity matrix | BLOSUM-62 |
5095 | | SS fraction | 0.3 |
5096 | | Gap open (HH/SS/other) | 18/18/6 |
5097 | | Gap extend | 1 |
5098 | | SS matrix | | | H | S | O |
5099 | | ---|---|---|--- |
5100 | | H | 6 | -9 | -6 |
5101 | | S | | 6 | -6 |
5102 | | O | | | 4 |
5103 | | Iteration cutoff | 2 |
5104 | | |
5105 | | Matchmaker CopA-F8WHL2.pdb, chain A (#34) with |
5106 | | fold_2025_01_17_alpha1_330gamma1_330zeta_model_0.cif, chain B (#40), sequence |
5107 | | alignment score = 1723 |
5108 | | RMSD between 297 pruned atom pairs is 0.381 angstroms; (across all 330 pairs: |
5109 | | 4.425) |
5110 | | |
5111 | | |
5112 | | > select subtract #40 |
5113 | | |
5114 | | Nothing selected |
5115 | | |
5116 | | > fitmap #40 inMap #25 |
5117 | | |
5118 | | Fit molecule fold_2025_01_17_alpha1_330gamma1_330zeta_model_0.cif (#40) to map |
5119 | | W20_J4027_005_volume_map_gammazeta_local.mrc (#25) using 6676 atoms |
5120 | | average map value = 0.2327, steps = 120 |
5121 | | shifted from previous position = 12.5 |
5122 | | rotated from previous position = 19.1 degrees |
5123 | | atoms outside contour = 3778, contour level = 0.2532 |
5124 | | |
5125 | | Position of fold_2025_01_17_alpha1_330gamma1_330zeta_model_0.cif (#40) |
5126 | | relative to W20_J4027_005_volume_map_gammazeta_local.mrc (#25) coordinates: |
5127 | | Matrix rotation and translation |
5128 | | 0.54165871 0.66183244 -0.51825058 133.50428914 |
5129 | | 0.57871718 -0.74075578 -0.34112650 139.20476253 |
5130 | | -0.60966570 -0.11514637 -0.78425063 100.42690475 |
5131 | | Axis 0.87742311 0.35494156 -0.32271532 |
5132 | | Axis point 0.00000000 37.30416243 78.13952669 |
5133 | | Rotation angle (degrees) 172.60119629 |
5134 | | Shift along axis 134.14000391 |
5135 | | |
5136 | | |
5137 | | > ui tool show Matchmaker |
5138 | | |
5139 | | > matchmaker #39 to #40 |
5140 | | |
5141 | | Computing secondary structure |
5142 | | [Repeated 1 time(s)] Parameters |
5143 | | --- |
5144 | | Chain pairing | bb |
5145 | | Alignment algorithm | Needleman-Wunsch |
5146 | | Similarity matrix | BLOSUM-62 |
5147 | | SS fraction | 0.3 |
5148 | | Gap open (HH/SS/other) | 18/18/6 |
5149 | | Gap extend | 1 |
5150 | | SS matrix | | | H | S | O |
5151 | | ---|---|---|--- |
5152 | | H | 6 | -9 | -6 |
5153 | | S | | 6 | -6 |
5154 | | O | | | 4 |
5155 | | Iteration cutoff | 2 |
5156 | | |
5157 | | Matchmaker fold_2025_01_17_alpha1_330gamma1_330zeta_model_0.cif, chain C (#40) |
5158 | | with CopZ1_P61924.pdb, chain A (#39), sequence alignment score = 863.4 |
5159 | | RMSD between 141 pruned atom pairs is 0.407 angstroms; (across all 177 pairs: |
5160 | | 16.666) |
5161 | | |
5162 | | |
5163 | | > hide #40 models |
5164 | | |
5165 | | > fitmap #39 inMap #25 |
5166 | | |
5167 | | Fit molecule CopZ1_P61924.pdb (#39) to map |
5168 | | W20_J4027_005_volume_map_gammazeta_local.mrc (#25) using 1420 atoms |
5169 | | average map value = 0.237, steps = 448 |
5170 | | shifted from previous position = 13.1 |
5171 | | rotated from previous position = 65.5 degrees |
5172 | | atoms outside contour = 774, contour level = 0.2532 |
5173 | | |
5174 | | Position of CopZ1_P61924.pdb (#39) relative to |
5175 | | W20_J4027_005_volume_map_gammazeta_local.mrc (#25) coordinates: |
5176 | | Matrix rotation and translation |
5177 | | 0.40316931 0.69456576 0.59584639 114.25318729 |
5178 | | -0.23944244 -0.54836159 0.80122836 139.81166245 |
5179 | | 0.88324506 -0.46570160 -0.05477404 99.19539890 |
5180 | | Axis -0.79181872 -0.17962133 -0.58374591 |
5181 | | Axis point 0.00000000 56.29130506 -26.82207038 |
5182 | | Rotation angle (degrees) 126.86869176 |
5183 | | Shift along axis -173.48587819 |
5184 | | |
5185 | | |
5186 | | > undo |
5187 | | |
5188 | | > hide #39 models |
5189 | | |
5190 | | > hide #38 models |
5191 | | |
5192 | | > hide #37 models |
5193 | | |
5194 | | > hide #36 models |
5195 | | |
5196 | | > hide #35 models |
5197 | | |
5198 | | > hide #34 models |
5199 | | |
5200 | | > hide #!25 models |
5201 | | |
5202 | | > ui mousemode right select |
5203 | | |
5204 | | Drag select of 102 residues |
5205 | | |
5206 | | > select up |
5207 | | |
5208 | | 1089 atoms, 1111 bonds, 132 residues, 1 model selected |
5209 | | |
5210 | | > select up |
5211 | | |
5212 | | 2689 atoms, 2767 bonds, 330 residues, 1 model selected |
5213 | | |
5214 | | > delete sel |
5215 | | |
5216 | | > fitmap #40 inMap #25 |
5217 | | |
5218 | | Fit molecule fold_2025_01_17_alpha1_330gamma1_330zeta_model_0.cif (#40) to map |
5219 | | W20_J4027_005_volume_map_gammazeta_local.mrc (#25) using 3987 atoms |
5220 | | average map value = 0.2376, steps = 148 |
5221 | | shifted from previous position = 8.47 |
5222 | | rotated from previous position = 22.7 degrees |
5223 | | atoms outside contour = 2154, contour level = 0.2532 |
5224 | | |
5225 | | Position of fold_2025_01_17_alpha1_330gamma1_330zeta_model_0.cif (#40) |
5226 | | relative to W20_J4027_005_volume_map_gammazeta_local.mrc (#25) coordinates: |
5227 | | Matrix rotation and translation |
5228 | | 0.25729822 0.88581488 -0.38617305 122.95584280 |
5229 | | 0.78223190 -0.42556216 -0.45498364 149.35405458 |
5230 | | -0.56737191 -0.18501039 -0.80240904 100.43354571 |
5231 | | Axis 0.79114243 0.53099377 -0.30354451 |
5232 | | Axis point 0.00000000 25.55099714 77.70315234 |
5233 | | Rotation angle (degrees) 170.17599112 |
5234 | | Shift along axis 146.09560633 |
5235 | | |
5236 | | |
5237 | | > show #!25 models |
5238 | | |
5239 | | > ui tool show Matchmaker |
5240 | | |
5241 | | > matchmaker #39 to #40 |
5242 | | |
5243 | | Computing secondary structure |
5244 | | [Repeated 1 time(s)] Parameters |
5245 | | --- |
5246 | | Chain pairing | bb |
5247 | | Alignment algorithm | Needleman-Wunsch |
5248 | | Similarity matrix | BLOSUM-62 |
5249 | | SS fraction | 0.3 |
5250 | | Gap open (HH/SS/other) | 18/18/6 |
5251 | | Gap extend | 1 |
5252 | | SS matrix | | | H | S | O |
5253 | | ---|---|---|--- |
5254 | | H | 6 | -9 | -6 |
5255 | | S | | 6 | -6 |
5256 | | O | | | 4 |
5257 | | Iteration cutoff | 2 |
5258 | | |
5259 | | Matchmaker fold_2025_01_17_alpha1_330gamma1_330zeta_model_0.cif, chain C (#40) |
5260 | | with CopZ1_P61924.pdb, chain A (#39), sequence alignment score = 863.4 |
5261 | | RMSD between 141 pruned atom pairs is 0.407 angstroms; (across all 177 pairs: |
5262 | | 16.666) |
5263 | | |
5264 | | |
5265 | | > show #39 models |
5266 | | |
5267 | | > hide #40 models |
5268 | | |
5269 | | > show #38 models |
5270 | | |
5271 | | > show #34 models |
5272 | | |
5273 | | > hide #39 models |
5274 | | |
5275 | | > hide #38 models |
5276 | | |
5277 | | > hide #!25 models |
5278 | | |
5279 | | Drag select of 84 residues |
5280 | | |
5281 | | > select up |
5282 | | |
5283 | | 956 atoms, 971 bonds, 118 residues, 1 model selected |
5284 | | |
5285 | | > select up |
5286 | | |
5287 | | 9810 atoms, 10032 bonds, 1233 residues, 1 model selected |
5288 | | |
5289 | | > select down |
5290 | | |
5291 | | 956 atoms, 971 bonds, 118 residues, 1 model selected |
5292 | | Drag select of 309 residues |
5293 | | |
5294 | | > delete sel |
5295 | | |
5296 | | > select clear |
5297 | | |
5298 | | [Repeated 2 time(s)]Drag select of 1 pseudobonds |
5299 | | |
5300 | | > delete sel |
5301 | | |
5302 | | > select add #34 |
5303 | | |
5304 | | 7345 atoms, 7512 bonds, 2 pseudobonds, 924 residues, 2 models selected |
5305 | | |
5306 | | > select subtract #34 |
5307 | | |
5308 | | Nothing selected |
5309 | | |
5310 | | > delete sel |
5311 | | |
5312 | | > close #34 |
5313 | | |
5314 | | > open "/Users/becca/Desktop/Postdoc/COPI notebooks and resources/Structure |
5315 | | > files/alphafold/CopA-F8WHL2.pdb" |
5316 | | |
5317 | | CopA-F8WHL2.pdb title: |
5318 | | Alphafold monomer V2.0 prediction for coatomer subunit α (F8WHL2) [more |
5319 | | info...] |
5320 | | |
5321 | | Chain information for CopA-F8WHL2.pdb #34 |
5322 | | --- |
5323 | | Chain | Description | UniProt |
5324 | | A | coatomer subunit α | F8WHL2_MOUSE 1-1233 |
5325 | | |
5326 | | Computing secondary structure |
5327 | | |
5328 | | > show #!25 models |
5329 | | |
5330 | | > select add #34 |
5331 | | |
5332 | | 9810 atoms, 10032 bonds, 1233 residues, 1 model selected |
5333 | | |
5334 | | > color sel navy |
5335 | | |
5336 | | > ui mousemode right "translate selected models" |
5337 | | |
5338 | | > view matrix models #34,1,0,0,139.38,0,1,0,77.792,0,0,1,174.15 |
5339 | | |
5340 | | > view matrix models #34,1,0,0,143.17,0,1,0,116.78,0,0,1,157.74 |
5341 | | |
5342 | | > ui mousemode right "rotate selected models" |
5343 | | |
5344 | | > view matrix models |
5345 | | > #34,-0.77292,0.46571,-0.43094,110.33,-0.63189,-0.50329,0.58942,111.94,0.057612,0.72788,0.68328,155.75 |
5346 | | |
5347 | | > view matrix models |
5348 | | > #34,0.095488,0.54565,-0.83256,128.67,-0.72338,-0.53652,-0.43459,118.09,-0.68382,0.64375,0.34348,145.26 |
5349 | | |
5350 | | > view matrix models |
5351 | | > #34,0.86645,-0.32341,0.38035,140.29,0.26177,0.943,0.20551,120.49,-0.42513,-0.078497,0.90172,151.23 |
5352 | | |
5353 | | > view matrix models |
5354 | | > #34,-0.13249,-0.98099,-0.14179,130.68,-0.29114,-0.098228,0.95162,112.52,-0.94746,0.16737,-0.27259,148.51 |
5355 | | |
5356 | | > view matrix models |
5357 | | > #34,-0.191,-0.9337,-0.30287,130.44,-0.10946,-0.28637,0.95185,117.25,-0.97547,0.21495,-0.047502,145.98 |
5358 | | |
5359 | | > view matrix models |
5360 | | > #34,-0.60871,-0.63739,-0.47244,121.83,0.33431,-0.74608,0.57585,131.6,-0.71952,0.19258,0.66723,145.55 |
5361 | | |
5362 | | > ui mousemode right "translate selected models" |
5363 | | |
5364 | | > view matrix models |
5365 | | > #34,-0.60871,-0.63739,-0.47244,153.15,0.33431,-0.74608,0.57585,151.65,-0.71952,0.19258,0.66723,153.4 |
5366 | | |
5367 | | > view matrix models |
5368 | | > #34,-0.60871,-0.63739,-0.47244,154.56,0.33431,-0.74608,0.57585,133.83,-0.71952,0.19258,0.66723,140.34 |
5369 | | |
5370 | | > ui mousemode right "rotate selected models" |
5371 | | |
5372 | | > view matrix models |
5373 | | > #34,-0.61566,-0.09401,-0.78238,152.7,0.38273,-0.90356,-0.1926,141.58,-0.68882,-0.41802,0.59227,145.99 |
5374 | | |
5375 | | > ui mousemode right "translate selected models" |
5376 | | |
5377 | | > view matrix models |
5378 | | > #34,-0.61566,-0.09401,-0.78238,154.2,0.38273,-0.90356,-0.1926,117.01,-0.68882,-0.41802,0.59227,135.94 |
5379 | | |
5380 | | > select up |
5381 | | |
5382 | | 40776 atoms, 41545 bonds, 5160 residues, 68 models selected |
5383 | | |
5384 | | > select down |
5385 | | |
5386 | | 9810 atoms, 10032 bonds, 1233 residues, 1 model selected |
5387 | | |
5388 | | > ui mousemode right select |
5389 | | |
5390 | | > select clear |
5391 | | |
5392 | | > fitmap #34 inMap #25 |
5393 | | |
5394 | | Fit molecule CopA-F8WHL2.pdb (#34) to map |
5395 | | W20_J4027_005_volume_map_gammazeta_local.mrc (#25) using 9810 atoms |
5396 | | average map value = 0.2513, steps = 268 |
5397 | | shifted from previous position = 12.5 |
5398 | | rotated from previous position = 28.3 degrees |
5399 | | atoms outside contour = 4976, contour level = 0.2532 |
5400 | | |
5401 | | Position of CopA-F8WHL2.pdb (#34) relative to |
5402 | | W20_J4027_005_volume_map_gammazeta_local.mrc (#25) coordinates: |
5403 | | Matrix rotation and translation |
5404 | | -0.53264139 -0.54606136 -0.64661437 159.40933932 |
5405 | | 0.58383331 -0.79018975 0.18638357 127.28571811 |
5406 | | -0.61272491 -0.27823941 0.73969657 133.57411156 |
5407 | | Axis -0.38016417 -0.02772906 0.92450327 |
5408 | | Axis point 83.38396978 99.21518793 0.00000000 |
5409 | | Rotation angle (degrees) 142.33221845 |
5410 | | Shift along axis 59.35847140 |
5411 | | |
5412 | | |
5413 | | > hide #!25 models |
5414 | | |
5415 | | Drag select of 204 residues |
5416 | | |
5417 | | > select up |
5418 | | |
5419 | | 1872 atoms, 1904 bonds, 233 residues, 1 model selected |
5420 | | |
5421 | | > delete sel |
5422 | | |
5423 | | Drag select of 67 residues, 2 pseudobonds |
5424 | | Drag select of 44 residues, 3 pseudobonds |
5425 | | |
5426 | | > select up |
5427 | | |
5428 | | 515 atoms, 525 bonds, 3 pseudobonds, 65 residues, 2 models selected |
5429 | | |
5430 | | > select up |
5431 | | |
5432 | | 7938 atoms, 8122 bonds, 3 pseudobonds, 1000 residues, 2 models selected |
5433 | | |
5434 | | > select down |
5435 | | |
5436 | | 515 atoms, 525 bonds, 3 pseudobonds, 65 residues, 2 models selected |
5437 | | Drag select of 62 residues, 3 pseudobonds |
5438 | | |
5439 | | > select up |
5440 | | |
5441 | | 597 atoms, 611 bonds, 3 pseudobonds, 76 residues, 2 models selected |
5442 | | |
5443 | | > delete sel |
5444 | | |
5445 | | Drag select of 2 residues |
5446 | | |
5447 | | > select up |
5448 | | |
5449 | | 645 atoms, 657 bonds, 90 residues, 1 model selected |
5450 | | |
5451 | | > delete sel |
5452 | | |
5453 | | > show #!25 models |
5454 | | |
5455 | | > fitmap #34 inMap #25 |
5456 | | |
5457 | | Fit molecule CopA-F8WHL2.pdb (#34) to map |
5458 | | W20_J4027_005_volume_map_gammazeta_local.mrc (#25) using 6696 atoms |
5459 | | average map value = 0.33, steps = 92 |
5460 | | shifted from previous position = 0.363 |
5461 | | rotated from previous position = 1.55 degrees |
5462 | | atoms outside contour = 2309, contour level = 0.2532 |
5463 | | |
5464 | | Position of CopA-F8WHL2.pdb (#34) relative to |
5465 | | W20_J4027_005_volume_map_gammazeta_local.mrc (#25) coordinates: |
5466 | | Matrix rotation and translation |
5467 | | -0.55114104 -0.52745699 -0.64655447 159.68929082 |
5468 | | 0.57420657 -0.80195943 0.16476617 126.61771889 |
5469 | | -0.60541752 -0.28044643 0.74486202 133.68887023 |
5470 | | Axis -0.37446301 -0.03459979 0.92659609 |
5471 | | Axis point 83.97786314 98.17305376 0.00000000 |
5472 | | Rotation angle (degrees) 143.52527944 |
5473 | | Shift along axis 59.69690535 |
5474 | | |
5475 | | |
5476 | | > hide #!34 models |
5477 | | |
5478 | | > show #35 models |
5479 | | |
5480 | | > fitmap #35 inMap #25 |
5481 | | |
5482 | | Fit molecule CopB_ Q9JIF7.pdb (#35) to map |
5483 | | W20_J4027_005_volume_map_gammazeta_local.mrc (#25) using 7501 atoms |
5484 | | average map value = 0.2378, steps = 84 |
5485 | | shifted from previous position = 1.34 |
5486 | | rotated from previous position = 3.2 degrees |
5487 | | atoms outside contour = 4024, contour level = 0.2532 |
5488 | | |
5489 | | Position of CopB_ Q9JIF7.pdb (#35) relative to |
5490 | | W20_J4027_005_volume_map_gammazeta_local.mrc (#25) coordinates: |
5491 | | Matrix rotation and translation |
5492 | | 0.32064619 0.78921696 -0.52375815 142.21372577 |
5493 | | 0.68088169 -0.57645114 -0.45177892 148.04005904 |
5494 | | -0.65847257 -0.21175615 -0.72220024 154.69062564 |
5495 | | Axis 0.81144623 0.45542974 -0.36624960 |
5496 | | Axis point 0.00000000 24.90601335 113.58992692 |
5497 | | Rotation angle (degrees) 171.49485982 |
5498 | | Shift along axis 126.16525710 |
5499 | | |
5500 | | |
5501 | | > fitmap #35 inMap #25 |
5502 | | |
5503 | | Fit molecule CopB_ Q9JIF7.pdb (#35) to map |
5504 | | W20_J4027_005_volume_map_gammazeta_local.mrc (#25) using 7501 atoms |
5505 | | average map value = 0.2378, steps = 48 |
5506 | | shifted from previous position = 0.00712 |
5507 | | rotated from previous position = 0.00209 degrees |
5508 | | atoms outside contour = 4022, contour level = 0.2532 |
5509 | | |
5510 | | Position of CopB_ Q9JIF7.pdb (#35) relative to |
5511 | | W20_J4027_005_volume_map_gammazeta_local.mrc (#25) coordinates: |
5512 | | Matrix rotation and translation |
5513 | | 0.32064133 0.78920396 -0.52378071 142.21243830 |
5514 | | 0.68089354 -0.57645720 -0.45175334 148.03333799 |
5515 | | -0.65846269 -0.21178811 -0.72219988 154.68860774 |
5516 | | Axis 0.81144528 0.45542871 -0.36625298 |
5517 | | Axis point 0.00000000 24.90541120 113.58774992 |
5518 | | Rotation angle (degrees) 171.49690419 |
5519 | | Shift along axis 126.16107988 |
5520 | | |
5521 | | |
5522 | | > hide #35 models |
5523 | | |
5524 | | > show #36 models |
5525 | | |
5526 | | > fitmap #36 inMap #25 |
5527 | | |
5528 | | Fit molecule CopBprime_O55029.pdb (#36) to map |
5529 | | W20_J4027_005_volume_map_gammazeta_local.mrc (#25) using 7214 atoms |
5530 | | average map value = 0.1828, steps = 80 |
5531 | | shifted from previous position = 1.71 |
5532 | | rotated from previous position = 0.68 degrees |
5533 | | atoms outside contour = 6397, contour level = 0.2532 |
5534 | | |
5535 | | Position of CopBprime_O55029.pdb (#36) relative to |
5536 | | W20_J4027_005_volume_map_gammazeta_local.mrc (#25) coordinates: |
5537 | | Matrix rotation and translation |
5538 | | 0.50825278 0.05183494 0.85964658 125.87415961 |
5539 | | 0.66191789 0.61506689 -0.42843603 193.30517521 |
5540 | | -0.55094811 0.78676926 0.27829898 176.59370632 |
5541 | | Axis 0.62023665 0.71996274 0.31138426 |
5542 | | Axis point 57.84080416 0.00000000 83.07791765 |
5543 | | Rotation angle (degrees) 78.41570987 |
5544 | | Shift along axis 272.23279077 |
5545 | | |
5546 | | |
5547 | | > hide #36 models |
5548 | | |
5549 | | > show #36 models |
5550 | | |
5551 | | > hide #36 models |
5552 | | |
5553 | | > show #37 models |
5554 | | |
5555 | | > hide #!25 models |
5556 | | |
5557 | | Drag select of 74 residues |
5558 | | |
5559 | | > select up |
5560 | | |
5561 | | 696 atoms, 711 bonds, 90 residues, 1 model selected |
5562 | | |
5563 | | > delete sel |
5564 | | |
5565 | | > select clear |
5566 | | |
5567 | | Drag select of 39 residues |
5568 | | |
5569 | | > select up |
5570 | | |
5571 | | 652 atoms, 661 bonds, 80 residues, 1 model selected |
5572 | | |
5573 | | > delete sel |
5574 | | |
5575 | | Drag select of 72 residues, 2 pseudobonds |
5576 | | |
5577 | | > delete sel |
5578 | | |
5579 | | > show #!25 models |
5580 | | |
5581 | | > select add #25 |
5582 | | |
5583 | | 2 models selected |
5584 | | |
5585 | | > select subtract #25 |
5586 | | |
5587 | | Nothing selected |
5588 | | |
5589 | | > select add #37 |
5590 | | |
5591 | | 2098 atoms, 2129 bonds, 269 residues, 1 model selected |
5592 | | Drag select of 25 W20_J4027_005_volume_map_gammazeta_local.mrc , 10 residues |
5593 | | |
5594 | | > select clear |
5595 | | |
5596 | | > select add #37 |
5597 | | |
5598 | | 2098 atoms, 2129 bonds, 269 residues, 1 model selected |
5599 | | |
5600 | | > ui mousemode right "translate selected models" |
5601 | | |
5602 | | > view matrix models |
5603 | | > #37,0.52381,0.45458,-0.7204,159.74,-0.84399,0.16247,-0.51115,164.47,-0.11532,0.87576,0.46877,118.38 |
5604 | | |
5605 | | > view matrix models |
5606 | | > #37,0.52381,0.45458,-0.7204,159.87,-0.84399,0.16247,-0.51115,160.32,-0.11532,0.87576,0.46877,115.66 |
5607 | | |
5608 | | > ui mousemode right "rotate selected models" |
5609 | | |
5610 | | > view matrix models |
5611 | | > #37,-0.51843,-0.78578,-0.33732,156.47,0.64386,-0.0991,-0.7587,177.15,0.56274,-0.61052,0.55731,131.39 |
5612 | | |
5613 | | > ui mousemode right "translate selected models" |
5614 | | |
5615 | | > view matrix models |
5616 | | > #37,-0.51843,-0.78578,-0.33732,156.82,0.64386,-0.0991,-0.7587,177.21,0.56274,-0.61052,0.55731,131.11 |
5617 | | |
5618 | | > view matrix models |
5619 | | > #37,-0.51843,-0.78578,-0.33732,169.14,0.64386,-0.0991,-0.7587,188.43,0.56274,-0.61052,0.55731,154.61 |
5620 | | |
5621 | | > view matrix models |
5622 | | > #37,-0.51843,-0.78578,-0.33732,168.55,0.64386,-0.0991,-0.7587,185.71,0.56274,-0.61052,0.55731,156.17 |
5623 | | |
5624 | | > fitmap #37 inMap #25 |
5625 | | |
5626 | | Fit molecule CopD_full_Q5XJY5.pdb (#37) to map |
5627 | | W20_J4027_005_volume_map_gammazeta_local.mrc (#25) using 2098 atoms |
5628 | | average map value = 0.1523, steps = 264 |
5629 | | shifted from previous position = 11.8 |
5630 | | rotated from previous position = 42.5 degrees |
5631 | | atoms outside contour = 1552, contour level = 0.2532 |
5632 | | |
5633 | | Position of CopD_full_Q5XJY5.pdb (#37) relative to |
5634 | | W20_J4027_005_volume_map_gammazeta_local.mrc (#25) coordinates: |
5635 | | Matrix rotation and translation |
5636 | | 0.13421510 -0.97879473 -0.15474878 179.22768371 |
5637 | | 0.80299796 0.19893091 -0.56180136 174.64912074 |
5638 | | 0.58067252 -0.04886073 0.81266971 148.66115690 |
5639 | | Axis 0.25715468 -0.36869186 0.89327363 |
5640 | | Axis point -62.36776041 208.19731599 0.00000000 |
5641 | | Rotation angle (degrees) 85.81897760 |
5642 | | Shift along axis 114.49262034 |
5643 | | |
5644 | | |
5645 | | > ui mousemode right "rotate selected models" |
5646 | | |
5647 | | > view matrix models |
5648 | | > #37,0.17652,-0.98345,-0.040823,181.47,0.73295,0.15901,-0.66143,172.16,0.65698,0.086834,0.74889,148.4 |
5649 | | |
5650 | | > view matrix models |
5651 | | > #37,0.4517,-0.88251,-0.13091,183.25,0.50738,0.3748,-0.77595,166,0.73385,0.28407,0.61707,146.24 |
5652 | | |
5653 | | > view matrix models |
5654 | | > #37,0.19394,-0.76059,-0.61959,169.31,0.33983,0.64457,-0.68487,164.7,0.92028,-0.077732,0.38347,145.18 |
5655 | | |
5656 | | > ui mousemode right "translate selected models" |
5657 | | |
5658 | | > ui mousemode right "rotate selected models" |
5659 | | |
5660 | | > view matrix models |
5661 | | > #37,0.13058,-0.95382,-0.27052,176.06,0.41699,0.30039,-0.85784,163.27,0.89948,-0.00078766,0.43696,145.75 |
5662 | | |
5663 | | > ui mousemode right "translate selected models" |
5664 | | |
5665 | | > view matrix models |
5666 | | > #37,0.13058,-0.95382,-0.27052,175.12,0.41699,0.30039,-0.85784,169.65,0.89948,-0.00078766,0.43696,144.2 |
5667 | | |
5668 | | > view matrix models |
5669 | | > #37,0.13058,-0.95382,-0.27052,176.92,0.41699,0.30039,-0.85784,173.28,0.89948,-0.00078766,0.43696,142.71 |
5670 | | |
5671 | | > view matrix models |
5672 | | > #37,0.13058,-0.95382,-0.27052,176.07,0.41699,0.30039,-0.85784,171.32,0.89948,-0.00078766,0.43696,141.37 |
5673 | | |
5674 | | > ui mousemode right "rotate selected models" |
5675 | | |
5676 | | > view matrix models |
5677 | | > #37,0.98025,0.13893,0.14075,193.31,0.13603,0.042901,-0.98977,165.4,-0.14355,0.98937,0.023155,115.29 |
5678 | | |
5679 | | > view matrix models |
5680 | | > #37,-0.70422,-0.31127,-0.63811,154.89,0.39637,-0.91803,0.010376,192.21,-0.58904,-0.24562,0.76987,127.74 |
5681 | | |
5682 | | > view matrix models |
5683 | | > #37,-0.068744,0.46239,-0.88401,156.66,-0.96301,-0.26218,-0.062249,169.55,-0.26056,0.84703,0.46331,123 |
5684 | | |
5685 | | > ui mousemode right "translate selected models" |
5686 | | |
5687 | | > view matrix models |
5688 | | > #37,-0.068744,0.46239,-0.88401,155.46,-0.96301,-0.26218,-0.062249,163.71,-0.26056,0.84703,0.46331,114.14 |
5689 | | |
5690 | | > view matrix models |
5691 | | > #37,-0.068744,0.46239,-0.88401,155.61,-0.96301,-0.26218,-0.062249,167.76,-0.26056,0.84703,0.46331,108.27 |
5692 | | |
5693 | | > view matrix models |
5694 | | > #37,-0.068744,0.46239,-0.88401,151.7,-0.96301,-0.26218,-0.062249,167.34,-0.26056,0.84703,0.46331,109.96 |
5695 | | |
5696 | | > view matrix models |
5697 | | > #37,-0.068744,0.46239,-0.88401,148.05,-0.96301,-0.26218,-0.062249,168.6,-0.26056,0.84703,0.46331,113.26 |
5698 | | |
5699 | | > fitmap #37 inMap #25 |
5700 | | |
5701 | | Fit molecule CopD_full_Q5XJY5.pdb (#37) to map |
5702 | | W20_J4027_005_volume_map_gammazeta_local.mrc (#25) using 2098 atoms |
5703 | | average map value = 0.188, steps = 268 |
5704 | | shifted from previous position = 1.8 |
5705 | | rotated from previous position = 31.7 degrees |
5706 | | atoms outside contour = 1411, contour level = 0.2532 |
5707 | | |
5708 | | Position of CopD_full_Q5XJY5.pdb (#37) relative to |
5709 | | W20_J4027_005_volume_map_gammazeta_local.mrc (#25) coordinates: |
5710 | | Matrix rotation and translation |
5711 | | -0.10900924 0.75139291 -0.65078850 146.28424526 |
5712 | | -0.97991274 0.02875802 0.19734233 168.20699465 |
5713 | | 0.16699701 0.65922808 0.73316460 122.79115227 |
5714 | | Axis 0.23450114 -0.41519279 -0.87899042 |
5715 | | Axis point 141.38224991 -30.78701025 0.00000000 |
5716 | | Rotation angle (degrees) 99.99389905 |
5717 | | Shift along axis -143.46675599 |
5718 | | |
5719 | | |
5720 | | > select subtract #37 |
5721 | | |
5722 | | Nothing selected |
5723 | | |
5724 | | > hide #!37 models |
5725 | | |
5726 | | > open "/Users/becca/Desktop/Postdoc/COPI notebooks and resources/Alphafold |
5727 | | > predictions/alphafold 3 |
5728 | | > /fold_20240724_copbdg1z1_try2/fold_20240724_copbdg1z1_try2_model_0.cif" |
5729 | | |
5730 | | Chain information for fold_20240724_copbdg1z1_try2_model_0.cif #41 |
5731 | | --- |
5732 | | Chain | Description |
5733 | | A | . |
5734 | | B | . |
5735 | | C | . |
5736 | | D | . |
5737 | | |
5738 | | Computing secondary structure |
5739 | | |
5740 | | > select add #41 |
5741 | | |
5742 | | 19765 atoms, 20090 bonds, 2515 residues, 1 model selected |
5743 | | |
5744 | | > ui tool show Matchmaker |
5745 | | |
5746 | | > matchmaker #41 to #35 |
5747 | | |
5748 | | Computing secondary structure |
5749 | | [Repeated 1 time(s)] Parameters |
5750 | | --- |
5751 | | Chain pairing | bb |
5752 | | Alignment algorithm | Needleman-Wunsch |
5753 | | Similarity matrix | BLOSUM-62 |
5754 | | SS fraction | 0.3 |
5755 | | Gap open (HH/SS/other) | 18/18/6 |
5756 | | Gap extend | 1 |
5757 | | SS matrix | | | H | S | O |
5758 | | ---|---|---|--- |
5759 | | H | 6 | -9 | -6 |
5760 | | S | | 6 | -6 |
5761 | | O | | | 4 |
5762 | | Iteration cutoff | 2 |
5763 | | |
5764 | | Matchmaker CopB_ Q9JIF7.pdb, chain A (#35) with |
5765 | | fold_20240724_copbdg1z1_try2_model_0.cif, chain A (#41), sequence alignment |
5766 | | score = 4801.7 |
5767 | | RMSD between 351 pruned atom pairs is 0.825 angstroms; (across all 953 pairs: |
5768 | | 12.162) |
5769 | | |
5770 | | |
5771 | | > fitmap #41 inMap #25 |
5772 | | |
5773 | | Fit molecule fold_20240724_copbdg1z1_try2_model_0.cif (#41) to map |
5774 | | W20_J4027_005_volume_map_gammazeta_local.mrc (#25) using 19765 atoms |
5775 | | average map value = 0.1792, steps = 108 |
5776 | | shifted from previous position = 5.04 |
5777 | | rotated from previous position = 8.73 degrees |
5778 | | atoms outside contour = 12927, contour level = 0.2532 |
5779 | | |
5780 | | Position of fold_20240724_copbdg1z1_try2_model_0.cif (#41) relative to |
5781 | | W20_J4027_005_volume_map_gammazeta_local.mrc (#25) coordinates: |
5782 | | Matrix rotation and translation |
5783 | | 0.76300462 0.02891891 -0.64574581 129.45795402 |
5784 | | 0.59375195 -0.42623762 0.68248085 141.01253356 |
5785 | | -0.25550456 -0.90414887 -0.34239193 134.21937577 |
5786 | | Axis -0.91776836 -0.22573072 0.32672141 |
5787 | | Axis point 0.00000000 107.57830339 53.51898785 |
5788 | | Rotation angle (degrees) 120.18624651 |
5789 | | Shift along axis -106.79093220 |
5790 | | |
5791 | | |
5792 | | > hide #!25 models |
5793 | | |
5794 | | > view matrix models |
5795 | | > #41,0.75987,0.021282,-0.64972,128.96,0.5945,-0.42707,0.6813,141.3,-0.26298,-0.90397,-0.33717,134.98 |
5796 | | |
5797 | | > ui mousemode right select |
5798 | | |
5799 | | > select clear |
5800 | | |
5801 | | > select #37/A |
5802 | | |
5803 | | 2098 atoms, 2129 bonds, 269 residues, 1 model selected |
5804 | | |
5805 | | > select #41/A |
5806 | | |
5807 | | 7501 atoms, 7619 bonds, 953 residues, 1 model selected |
5808 | | |
5809 | | > select #41/D |
5810 | | |
5811 | | 1420 atoms, 1441 bonds, 177 residues, 1 model selected |
5812 | | |
5813 | | > delete sel |
5814 | | |
5815 | | > select #41/C |
5816 | | |
5817 | | 6830 atoms, 6947 bonds, 874 residues, 1 model selected |
5818 | | |
5819 | | > delete sel |
5820 | | |
5821 | | > fitmap #41 inMap #25 |
5822 | | |
5823 | | Fit molecule fold_20240724_copbdg1z1_try2_model_0.cif (#41) to map |
5824 | | W20_J4027_005_volume_map_gammazeta_local.mrc (#25) using 11515 atoms |
5825 | | average map value = 0.1733, steps = 152 |
5826 | | shifted from previous position = 7.6 |
5827 | | rotated from previous position = 10.2 degrees |
5828 | | atoms outside contour = 7595, contour level = 0.2532 |
5829 | | |
5830 | | Position of fold_20240724_copbdg1z1_try2_model_0.cif (#41) relative to |
5831 | | W20_J4027_005_volume_map_gammazeta_local.mrc (#25) coordinates: |
5832 | | Matrix rotation and translation |
5833 | | 0.72372649 0.02528009 -0.68962373 129.00641102 |
5834 | | 0.66961476 -0.26733223 0.69292825 143.29008926 |
5835 | | -0.16684136 -0.96327275 -0.21040333 139.88692109 |
5836 | | Axis -0.89407287 -0.28221546 0.34783348 |
5837 | | Axis point 0.00000000 117.92026971 52.51002968 |
5838 | | Rotation angle (degrees) 112.14825963 |
5839 | | Shift along axis -107.12245762 |
5840 | | |
5841 | | |
5842 | | > show #!25 models |
5843 | | |
5844 | | > hide #!25 models |
5845 | | |
5846 | | Drag select of 19 residues |
5847 | | |
5848 | | > select up |
5849 | | |
5850 | | 374 atoms, 380 bonds, 50 residues, 1 model selected |
5851 | | |
5852 | | > select up |
5853 | | |
5854 | | 4014 atoms, 4083 bonds, 511 residues, 1 model selected |
5855 | | |
5856 | | > select down |
5857 | | |
5858 | | 374 atoms, 380 bonds, 50 residues, 1 model selected |
5859 | | |
5860 | | > delete sel |
5861 | | |
5862 | | Drag select of 24 residues, 2 pseudobonds |
5863 | | |
5864 | | > select up |
5865 | | |
5866 | | 272 atoms, 278 bonds, 2 pseudobonds, 34 residues, 2 models selected |
5867 | | |
5868 | | > delete sel |
5869 | | |
5870 | | Drag select of 46 residues, 2 pseudobonds |
5871 | | |
5872 | | > select up |
5873 | | |
5874 | | 840 atoms, 846 bonds, 2 pseudobonds, 103 residues, 2 models selected |
5875 | | |
5876 | | > delete sel |
5877 | | |
5878 | | Drag select of 81 residues, 2 pseudobonds |
5879 | | Drag select of 82 residues, 2 pseudobonds |
5880 | | |
5881 | | > select clear |
5882 | | |
5883 | | Drag select of 53 residues |
5884 | | |
5885 | | > select up |
5886 | | |
5887 | | 483 atoms, 487 bonds, 61 residues, 1 model selected |
5888 | | |
5889 | | > select clear |
5890 | | |
5891 | | Drag select of 82 residues, 2 pseudobonds |
5892 | | |
5893 | | > select up |
5894 | | |
5895 | | 844 atoms, 855 bonds, 2 pseudobonds, 108 residues, 2 models selected |
5896 | | |
5897 | | > delete sel |
5898 | | |
5899 | | > fitmap #41 inMap #25 |
5900 | | |
5901 | | Fit molecule fold_20240724_copbdg1z1_try2_model_0.cif (#41) to map |
5902 | | W20_J4027_005_volume_map_gammazeta_local.mrc (#25) using 9185 atoms |
5903 | | average map value = 0.207, steps = 68 |
5904 | | shifted from previous position = 0.487 |
5905 | | rotated from previous position = 1.21 degrees |
5906 | | atoms outside contour = 5435, contour level = 0.2532 |
5907 | | |
5908 | | Position of fold_20240724_copbdg1z1_try2_model_0.cif (#41) relative to |
5909 | | W20_J4027_005_volume_map_gammazeta_local.mrc (#25) coordinates: |
5910 | | Matrix rotation and translation |
5911 | | 0.71958143 0.04416992 -0.69300187 128.82756784 |
5912 | | 0.67569054 -0.27468426 0.68409856 142.78127561 |
5913 | | -0.16014013 -0.96051943 -0.22750289 139.51471009 |
5914 | | Axis -0.89355970 -0.28951634 0.34312003 |
5915 | | Axis point 0.00000000 115.94606449 53.28137283 |
5916 | | Rotation angle (degrees) 113.03559189 |
5917 | | Shift along axis -108.58234297 |
5918 | | |
5919 | | |
5920 | | > show #!25 models |
5921 | | |
5922 | | > hide #!25 models |
5923 | | |
5924 | | Drag select of 248 residues |
5925 | | |
5926 | | > select up |
5927 | | |
5928 | | 2203 atoms, 2233 bonds, 279 residues, 1 model selected |
5929 | | |
5930 | | > delete sel |
5931 | | |
5932 | | > fitmap #41 inMap #25 |
5933 | | |
5934 | | Fit molecule fold_20240724_copbdg1z1_try2_model_0.cif (#41) to map |
5935 | | W20_J4027_005_volume_map_gammazeta_local.mrc (#25) using 6982 atoms |
5936 | | average map value = 0.2082, steps = 260 |
5937 | | shifted from previous position = 7.84 |
5938 | | rotated from previous position = 14 degrees |
5939 | | atoms outside contour = 4267, contour level = 0.2532 |
5940 | | |
5941 | | Position of fold_20240724_copbdg1z1_try2_model_0.cif (#41) relative to |
5942 | | W20_J4027_005_volume_map_gammazeta_local.mrc (#25) coordinates: |
5943 | | Matrix rotation and translation |
5944 | | 0.60641983 0.19676174 -0.77041535 127.17385378 |
5945 | | 0.79490418 -0.12618975 0.59346734 140.58903166 |
5946 | | 0.01955314 -0.97229675 -0.23293070 138.75917394 |
5947 | | Axis -0.84500929 -0.42632905 0.32280465 |
5948 | | Axis point 0.00000000 109.24582338 58.49339501 |
5949 | | Rotation angle (degrees) 112.10779478 |
5950 | | Shift along axis -122.60816993 |
5951 | | |
5952 | | |
5953 | | > show #!25 models |
5954 | | |
5955 | | > hide #!25 models |
5956 | | |
5957 | | Drag select of 85 residues, 1 pseudobonds |
5958 | | |
5959 | | > select up |
5960 | | |
5961 | | 826 atoms, 836 bonds, 1 pseudobond, 108 residues, 2 models selected |
5962 | | |
5963 | | > delete sel |
5964 | | |
5965 | | Drag select of 43 residues |
5966 | | |
5967 | | > select clear |
5968 | | |
5969 | | Drag select of 67 residues |
5970 | | |
5971 | | > select up |
5972 | | |
5973 | | 711 atoms, 719 bonds, 95 residues, 1 model selected |
5974 | | |
5975 | | > delete sel |
5976 | | |
5977 | | > fitmap #41 inMap #25 |
5978 | | |
5979 | | Fit molecule fold_20240724_copbdg1z1_try2_model_0.cif (#41) to map |
5980 | | W20_J4027_005_volume_map_gammazeta_local.mrc (#25) using 5445 atoms |
5981 | | average map value = 0.1782, steps = 88 |
5982 | | shifted from previous position = 2.03 |
5983 | | rotated from previous position = 3.11 degrees |
5984 | | atoms outside contour = 3752, contour level = 0.2532 |
5985 | | |
5986 | | Position of fold_20240724_copbdg1z1_try2_model_0.cif (#41) relative to |
5987 | | W20_J4027_005_volume_map_gammazeta_local.mrc (#25) coordinates: |
5988 | | Matrix rotation and translation |
5989 | | 0.60331496 0.23815668 -0.76111264 129.24175666 |
5990 | | 0.79728988 -0.15805568 0.58253519 139.72739672 |
5991 | | 0.01843647 -0.95827960 -0.28523727 137.10117129 |
5992 | | Axis -0.84890646 -0.42948982 0.30805246 |
5993 | | Axis point 0.00000000 103.56300999 60.01449132 |
5994 | | Rotation angle (degrees) 114.83389261 |
5995 | | Shift along axis -127.49130401 |
5996 | | |
5997 | | |
5998 | | > show #!25 models |
5999 | | |
6000 | | > select add #41 |
6001 | | |
6002 | | 5445 atoms, 5521 bonds, 8 pseudobonds, 687 residues, 2 models selected |
6003 | | Drag select of 25 W20_J4027_005_volume_map_gammazeta_local.mrc , 43 residues |
6004 | | |
6005 | | > select clear |
6006 | | |
6007 | | > select add #41 |
6008 | | |
6009 | | 5445 atoms, 5521 bonds, 8 pseudobonds, 687 residues, 2 models selected |
6010 | | |
6011 | | > ui mousemode right "translate selected models" |
6012 | | |
6013 | | > view matrix models |
6014 | | > #41,0.60241,0.22967,-0.76444,137.4,0.7981,-0.15867,0.58126,145.75,0.012205,-0.96025,-0.27888,129.95 |
6015 | | |
6016 | | > fitmap #41 inMap #25 |
6017 | | |
6018 | | Fit molecule fold_20240724_copbdg1z1_try2_model_0.cif (#41) to map |
6019 | | W20_J4027_005_volume_map_gammazeta_local.mrc (#25) using 5445 atoms |
6020 | | average map value = 0.203, steps = 116 |
6021 | | shifted from previous position = 3.37 |
6022 | | rotated from previous position = 14.2 degrees |
6023 | | atoms outside contour = 3158, contour level = 0.2532 |
6024 | | |
6025 | | Position of fold_20240724_copbdg1z1_try2_model_0.cif (#41) relative to |
6026 | | W20_J4027_005_volume_map_gammazeta_local.mrc (#25) coordinates: |
6027 | | Matrix rotation and translation |
6028 | | 0.52191157 0.05705493 -0.85108933 136.78168805 |
6029 | | 0.85298460 -0.02899341 0.52113016 146.75941736 |
6030 | | 0.00505707 -0.99794996 -0.06379897 132.92819195 |
6031 | | Axis -0.79251134 -0.44665565 0.41524030 |
6032 | | Axis point 0.00000000 120.55875518 58.21435828 |
6033 | | Rotation angle (degrees) 106.58517534 |
6034 | | Shift along axis -118.75481890 |
6035 | | |
6036 | | |
6037 | | > select subtract #41 |
6038 | | |
6039 | | Nothing selected |
6040 | | |
6041 | | > select add #41 |
6042 | | |
6043 | | 5445 atoms, 5521 bonds, 8 pseudobonds, 687 residues, 2 models selected |
6044 | | |
6045 | | > select subtract #41 |
6046 | | |
6047 | | Nothing selected |
6048 | | |
6049 | | > select add #41 |
6050 | | |
6051 | | 5445 atoms, 5521 bonds, 8 pseudobonds, 687 residues, 2 models selected |
6052 | | |
6053 | | > select subtract #41 |
6054 | | |
6055 | | Nothing selected |
6056 | | |
6057 | | > show #35 models |
6058 | | |
6059 | | > hide #35 models |
6060 | | |
6061 | | > ui tool show Matchmaker |
6062 | | |
6063 | | > matchmaker #!37 to #41 |
6064 | | |
6065 | | Computing secondary structure |
6066 | | [Repeated 1 time(s)] Parameters |
6067 | | --- |
6068 | | Chain pairing | bb |
6069 | | Alignment algorithm | Needleman-Wunsch |
6070 | | Similarity matrix | BLOSUM-62 |
6071 | | SS fraction | 0.3 |
6072 | | Gap open (HH/SS/other) | 18/18/6 |
6073 | | Gap extend | 1 |
6074 | | SS matrix | | | H | S | O |
6075 | | ---|---|---|--- |
6076 | | H | 6 | -9 | -6 |
6077 | | S | | 6 | -6 |
6078 | | O | | | 4 |
6079 | | Iteration cutoff | 2 |
6080 | | |
6081 | | Matchmaker fold_20240724_copbdg1z1_try2_model_0.cif, chain B (#41) with |
6082 | | CopD_full_Q5XJY5.pdb, chain A (#37), sequence alignment score = 2184.5 |
6083 | | RMSD between 114 pruned atom pairs is 0.700 angstroms; (across all 244 pairs: |
6084 | | 25.738) |
6085 | | |
6086 | | |
6087 | | > show #!37 models |
6088 | | |
6089 | | > show #35 models |
6090 | | |
6091 | | > hide #!41 models |
6092 | | |
6093 | | > hide #!25 models |
6094 | | |
6095 | | > ui mousemode right rotate |
6096 | | |
6097 | | > ui mousemode right select |
6098 | | |
6099 | | Drag select of 1 residues |
6100 | | |
6101 | | > select up |
6102 | | |
6103 | | 184 atoms, 186 bonds, 25 residues, 1 model selected |
6104 | | |
6105 | | > delete sel |
6106 | | |
6107 | | > show #!25 models |
6108 | | |
6109 | | Drag select of 6 residues |
6110 | | |
6111 | | > select up |
6112 | | |
6113 | | 483 atoms, 491 bonds, 69 residues, 1 model selected |
6114 | | |
6115 | | > delete sel |
6116 | | |
6117 | | > hide #!25 models |
6118 | | |
6119 | | > show #!25 models |
6120 | | |
6121 | | > show #!34 models |
6122 | | |
6123 | | > show #38 models |
6124 | | |
6125 | | > show #39 models |
6126 | | |
6127 | | > show #36 models |
6128 | | |
6129 | | > hide #39 models |
6130 | | |
6131 | | > hide #!37 models |
6132 | | |
6133 | | > hide #36 models |
6134 | | |
6135 | | > hide #35 models |
6136 | | |
6137 | | > hide #!34 models |
6138 | | |
6139 | | > hide #!25 models |
6140 | | |
6141 | | Drag select of 41 residues |
6142 | | |
6143 | | > select up |
6144 | | |
6145 | | 531 atoms, 541 bonds, 67 residues, 1 model selected |
6146 | | |
6147 | | > delete sel |
6148 | | |
6149 | | Drag select of 26 residues, 1 pseudobonds |
6150 | | |
6151 | | > select up |
6152 | | |
6153 | | 494 atoms, 500 bonds, 1 pseudobond, 64 residues, 2 models selected |
6154 | | |
6155 | | > delete sel |
6156 | | |
6157 | | Drag select of 3 residues |
6158 | | |
6159 | | > select up |
6160 | | |
6161 | | 215 atoms, 218 bonds, 30 residues, 1 model selected |
6162 | | |
6163 | | > delete sel |
6164 | | |
6165 | | Drag select of 43 residues, 1 pseudobonds |
6166 | | |
6167 | | > select up |
6168 | | |
6169 | | 499 atoms, 507 bonds, 1 pseudobond, 62 residues, 2 models selected |
6170 | | |
6171 | | > select clear |
6172 | | |
6173 | | Drag select of 23 residues |
6174 | | |
6175 | | > select up |
6176 | | |
6177 | | 296 atoms, 302 bonds, 36 residues, 1 model selected |
6178 | | Drag select of 51 residues, 2 pseudobonds |
6179 | | |
6180 | | > select clear |
6181 | | |
6182 | | Drag select of 19 residues, 3 pseudobonds |
6183 | | |
6184 | | > select up |
6185 | | |
6186 | | 167 atoms, 168 bonds, 3 pseudobonds, 20 residues, 2 models selected |
6187 | | |
6188 | | > delete sel |
6189 | | |
6190 | | > show #!25 models |
6191 | | |
6192 | | > hide #!25 models |
6193 | | |
6194 | | Drag select of 33 residues |
6195 | | |
6196 | | > select up |
6197 | | |
6198 | | 594 atoms, 601 bonds, 75 residues, 1 model selected |
6199 | | |
6200 | | > delete sel |
6201 | | |
6202 | | > show #!25 models |
6203 | | |
6204 | | > hide #!25 models |
6205 | | |
6206 | | Drag select of 65 residues, 3 pseudobonds |
6207 | | |
6208 | | > select up |
6209 | | |
6210 | | 603 atoms, 610 bonds, 3 pseudobonds, 79 residues, 2 models selected |
6211 | | |
6212 | | > delete sel |
6213 | | |
6214 | | > show #!25 models |
6215 | | |
6216 | | > fitmap #38 inMap #25 |
6217 | | |
6218 | | Fit molecule CopG1_ Q9QZE5.pdb (#38) to map |
6219 | | W20_J4027_005_volume_map_gammazeta_local.mrc (#25) using 4226 atoms |
6220 | | average map value = 0.2729, steps = 96 |
6221 | | shifted from previous position = 0.601 |
6222 | | rotated from previous position = 5.3 degrees |
6223 | | atoms outside contour = 1993, contour level = 0.2532 |
6224 | | |
6225 | | Position of CopG1_ Q9QZE5.pdb (#38) relative to |
6226 | | W20_J4027_005_volume_map_gammazeta_local.mrc (#25) coordinates: |
6227 | | Matrix rotation and translation |
6228 | | -0.43244711 0.82798862 0.35696546 106.28810019 |
6229 | | -0.18802207 0.30438532 -0.93380795 122.80007772 |
6230 | | -0.88183740 -0.47093993 0.02404958 119.35137932 |
6231 | | Axis 0.27755203 0.74283001 -0.60923595 |
6232 | | Axis point 83.94095393 0.00000000 76.41437643 |
6233 | | Rotation angle (degrees) 123.50474947 |
6234 | | Shift along axis 48.00691086 |
6235 | | |
6236 | | |
6237 | | > show #39 models |
6238 | | |
6239 | | > fitmap #39 inMap #25 |
6240 | | |
6241 | | Fit molecule CopZ1_P61924.pdb (#39) to map |
6242 | | W20_J4027_005_volume_map_gammazeta_local.mrc (#25) using 1420 atoms |
6243 | | average map value = 0.2375, steps = 180 |
6244 | | shifted from previous position = 1.19 |
6245 | | rotated from previous position = 45.5 degrees |
6246 | | atoms outside contour = 765, contour level = 0.2532 |
6247 | | |
6248 | | Position of CopZ1_P61924.pdb (#39) relative to |
6249 | | W20_J4027_005_volume_map_gammazeta_local.mrc (#25) coordinates: |
6250 | | Matrix rotation and translation |
6251 | | 0.15949015 0.95372824 0.25488301 116.12737830 |
6252 | | 0.29301360 -0.29228658 0.91033598 142.77739950 |
6253 | | 0.94271201 -0.07050544 -0.32607217 99.85061617 |
6254 | | Axis -0.71693601 -0.50276157 -0.48294261 |
6255 | | Axis point 0.00000000 31.13613117 -0.38312474 |
6256 | | Rotation angle (degrees) 136.83898997 |
6257 | | Shift along axis -203.26100587 |
6258 | | |
6259 | | |
6260 | | > undo |
6261 | | |
6262 | | > show #39 models |
6263 | | |
6264 | | > ui tool show Matchmaker |
6265 | | |
6266 | | > matchmaker #39 to #40 |
6267 | | |
6268 | | Computing secondary structure |
6269 | | [Repeated 1 time(s)] Parameters |
6270 | | --- |
6271 | | Chain pairing | bb |
6272 | | Alignment algorithm | Needleman-Wunsch |
6273 | | Similarity matrix | BLOSUM-62 |
6274 | | SS fraction | 0.3 |
6275 | | Gap open (HH/SS/other) | 18/18/6 |
6276 | | Gap extend | 1 |
6277 | | SS matrix | | | H | S | O |
6278 | | ---|---|---|--- |
6279 | | H | 6 | -9 | -6 |
6280 | | S | | 6 | -6 |
6281 | | O | | | 4 |
6282 | | Iteration cutoff | 2 |
6283 | | |
6284 | | Matchmaker fold_2025_01_17_alpha1_330gamma1_330zeta_model_0.cif, chain C (#40) |
6285 | | with CopZ1_P61924.pdb, chain A (#39), sequence alignment score = 863.4 |
6286 | | RMSD between 141 pruned atom pairs is 0.407 angstroms; (across all 177 pairs: |
6287 | | 16.666) |
6288 | | |
6289 | | |
6290 | | > select clear |
6291 | | |
6292 | | > show #!34 models |
6293 | | |
6294 | | > show #35 models |
6295 | | |
6296 | | > show #36 models |
6297 | | |
6298 | | > show #!37 models |
6299 | | |
6300 | | > save "/Users/becca/Desktop/Postdoc/COPI notebooks and |
6301 | | > resources/Processing/W20_MM_cyto_COPI/20250701_W20_COPI_cyto_alignments.cxs" |
6302 | | |
6303 | | > hide #!25 models |
6304 | | |
6305 | | > show #!26 models |
6306 | | |
6307 | | > hide #!26 models |
6308 | | |
6309 | | > show #!27 models |
6310 | | |
6311 | | > save "/Users/becca/Desktop/Postdoc/COPI notebooks and |
6312 | | > resources/Processing/W20_MM_cyto_COPI/20250701_W20_COPI_cyto_alignments.cxs" |
6313 | | |
6314 | | ——— End of log from Tue Jul 1 15:42:09 2025 ——— |
6315 | | |
6316 | | > view name session-start |
6317 | | |
6318 | | opened ChimeraX session |
6319 | | |
6320 | | > show #!1 models |
6321 | | |
6322 | | > hide #!1 models |
6323 | | |
6324 | | > hide #!27 models |
6325 | | |
6326 | | > show #!29 models |
6327 | | |
6328 | | > hide #!29 models |
6329 | | |
6330 | | > show #!29 models |
6331 | | |
6332 | | > volume #29.1 level 0.01218 |
6333 | | |
6334 | | > volume #29.2 level 0.009354 |
6335 | | |
6336 | | > volume #29.3 level 0.003817 |
6337 | | |
6338 | | > volume #29.4 level 0.01012 |
6339 | | |
6340 | | > hide #!29 models |
6341 | | |
6342 | | > show #!29 models |
6343 | | |
6344 | | > color #29 silver models |
6345 | | |
6346 | | > color #29 darkgrey models |
6347 | | |
6348 | | > color #29 #a9a9a9c7 models |
6349 | | |
6350 | | > color #29 darkgrey models |
6351 | | |
6352 | | > color #29 #929292ff models |
6353 | | |
6354 | | > color #29 darkgrey models |
6355 | | |
6356 | | > show #!30 models |
6357 | | |
6358 | | > volume #30.1 level 0.004859 |
6359 | | |
6360 | | > hide #!29 models |
6361 | | |
6362 | | > color #30 #929292ff models |
6363 | | |
6364 | | > volume #30.1 level 0.01271 |
6365 | | |
6366 | | > volume #30.2 level 0.00973 |
6367 | | |
6368 | | > volume #30.3 level 0.008159 |
6369 | | |
6370 | | > volume #30.4 level 0.01229 |
6371 | | |
6372 | | > hide #!30 models |
6373 | | |
6374 | | > show #!31 models |
6375 | | |
6376 | | > volume #31.1 level 0.01583 |
6377 | | |
6378 | | > color #31 #929292ff models |
6379 | | |
6380 | | > volume #31.2 level 0.0156 |
6381 | | |
6382 | | > volume #31.3 level 0.008589 |
6383 | | |
6384 | | > volume #31.4 level 0.01446 |
6385 | | |
6386 | | > hide #!31 models |
6387 | | |
6388 | | > color #32 #929292ff models |
6389 | | |
6390 | | > show #!32 models |
6391 | | |
6392 | | > volume #32.1 level 0.01368 |
6393 | | |
6394 | | > volume #32.3 level 0.01158 |
6395 | | |
6396 | | > volume #32.4 level 0.01325 |
6397 | | |
6398 | | > volume #32.2 level 0.01059 |
6399 | | |
6400 | | > hide #!32 models |
6401 | | |
6402 | | > show #!33 models |
6403 | | |
6404 | | > volume #33.1 level 0.01263 |
6405 | | |
6406 | | > color #33 #929292ff models |
6407 | | |
6408 | | > volume #33.1 level 0.01481 |
6409 | | |
6410 | | > volume #33.2 level 0.01481 |
6411 | | |
6412 | | > volume #33.3 level 0.01517 |
6413 | | |
6414 | | > volume #33.4 level 0.01344 |
6415 | | |
6416 | | > hide #!34 models |
6417 | | |
6418 | | > hide #35 models |
6419 | | |
6420 | | > hide #36 models |
6421 | | |
6422 | | > hide #!37 models |
6423 | | |
6424 | | > hide #!38 models |
6425 | | |
6426 | | > hide #39 models |
6427 | | |
6428 | | > show #!41 models |
6429 | | |
6430 | | > hide #!41 models |
6431 | | |
6432 | | > show #40 models |
6433 | | |
6434 | | > hide #40 models |
6435 | | |
6436 | | > show #!29 models |
6437 | | |
6438 | | > color #29 #8efa00ff models |
6439 | | |
6440 | | > color #29 #fffc79ff models |
6441 | | |
6442 | | > volume #29.1 level 0.01322 |
6443 | | |
6444 | | > volume #29.2 level 0.0117 |
6445 | | |
6446 | | > volume #29.3 level 0.01271 |
6447 | | |
6448 | | > volume #29.4 level 0.01296 |
6449 | | |
6450 | | > hide #!29 models |
6451 | | |
6452 | | > show #!29 models |
6453 | | |
6454 | | > hide #!33 models |
6455 | | |
6456 | | > show #!30 models |
6457 | | |
6458 | | > hide #!30 models |
6459 | | |
6460 | | > show #!31 models |
6461 | | |
6462 | | > hide #!31 models |
6463 | | |
6464 | | > show #!32 models |
6465 | | |
6466 | | > hide #!32 models |
6467 | | |
6468 | | > show #!33 models |
6469 | | |
6470 | | > show #!32 models |
6471 | | |
6472 | | > hide #!32 models |
6473 | | |
6474 | | > show #!32 models |
6475 | | |
6476 | | > hide #!32 models |
6477 | | |
6478 | | > show #!32 models |
6479 | | |
6480 | | > hide #!32 models |
6481 | | |
6482 | | > show #!32 models |
6483 | | |
6484 | | > hide #!33 models |
6485 | | |
6486 | | > show #!33 models |
6487 | | |
6488 | | > hide #!32 models |
6489 | | |
6490 | | > hide #!33 models |
6491 | | |
6492 | | > show #!30 models |
6493 | | |
6494 | | > hide #!29 models |
6495 | | |
6496 | | > volume maximum #30.1 #30.2 #30.3 #30.4 |
6497 | | |
6498 | | Opened volume maximum as #42, grid size 285,281,252, pixel 1.2, shown at step |
6499 | | 1, values float32 |
6500 | | |
6501 | | > rename #42 W20_J140_MB |
6502 | | |
6503 | | > hide #!42 models |
6504 | | |
6505 | | > show #!42 models |
6506 | | |
6507 | | > volume maximum #31.1 #31.2 #31.3 #31.4 |
6508 | | |
6509 | | Opened volume maximum as #43, grid size 248,255,253, pixel 1.2, shown at step |
6510 | | 1, values float32 |
6511 | | |
6512 | | > rename #43 W20_J145_MB |
6513 | | |
6514 | | > hide #!43 models |
6515 | | |
6516 | | > show #!43 models |
6517 | | |
6518 | | > volume maximum #32.1 #32.2 #32.3 #32.4 |
6519 | | |
6520 | | Opened volume maximum as #44, grid size 275,269,255, pixel 1.2, shown at step |
6521 | | 1, values float32 |
6522 | | |
6523 | | > rename #44 W20_J150_MB |
6524 | | |
6525 | | > hide #!44 models |
6526 | | |
6527 | | > show #!44 models |
6528 | | |
6529 | | > volume maximum #33.1 #33.2 #33.3 #33.4 |
6530 | | |
6531 | | Opened volume maximum as #45, grid size 299,295,317, pixel 1.2, shown at step |
6532 | | 1, values float32 |
6533 | | |
6534 | | > rename #45 W20_J155_MB |
6535 | | |
6536 | | > volume maximum #34.1 #34.2 #34.3 #34.4 |
6537 | | |
6538 | | [Repeated 3 time(s)] |
6539 | | |
6540 | | > close #42 |
6541 | | |
6542 | | > close #43 |
6543 | | |
6544 | | > close #44 |
6545 | | |
6546 | | > close #45 |
6547 | | |
6548 | | > volume maximum #34.1 #34.2 #34.3 #34.4 |
6549 | | |
6550 | | > volume maximum #29.1 #29.2 #29.3 #29.4 |
6551 | | |
6552 | | Opened volume maximum as #42, grid size 281,271,305, pixel 1.2, shown at step |
6553 | | 1, values float32 |
6554 | | |
6555 | | > volume maximum #30.1 #29.2 #29.3 #29.4 |
6556 | | |
6557 | | Opened volume maximum as #43, grid size 324,314,331, pixel 1.2, shown at step |
6558 | | 1, values float32 |
6559 | | |
6560 | | > close #43 |
6561 | | |
6562 | | > volume maximum #30.1 #30.2 #30.3 #30.4 |
6563 | | |
6564 | | Opened volume maximum as #43, grid size 285,281,252, pixel 1.2, shown at step |
6565 | | 1, values float32 |
6566 | | |
6567 | | > volume maximum #31.1 #31.2 #31.3 #31.4 |
6568 | | |
6569 | | Opened volume maximum as #44, grid size 248,255,253, pixel 1.2, shown at step |
6570 | | 1, values float32 |
6571 | | |
6572 | | > volume maximum #32.1 #32.2 #32.3 #32.4 |
6573 | | |
6574 | | Opened volume maximum as #45, grid size 275,269,255, pixel 1.2, shown at step |
6575 | | 1, values float32 |
6576 | | |
6577 | | > volume maximum #33.1 #33.2 #33.3 #33.4 |
6578 | | |
6579 | | Opened volume maximum as #46, grid size 299,295,317, pixel 1.2, shown at step |
6580 | | 1, values float32 |
6581 | | |
6582 | | > rename #42 W20_J140_MB |
6583 | | |
6584 | | > rename #42 W20_J140_MB_joined |
6585 | | |
6586 | | > rename #43 W20_J145_MB_joined |
6587 | | |
6588 | | > rename #44 W20_J150_MB_joined |
6589 | | |
6590 | | > rename #45 W20_J155_MB_joined |
6591 | | |
6592 | | > rename #46 W20_J160_MB_joined |
6593 | | |
6594 | | > fitmap #42 inMap #1 |
6595 | | |
6596 | | Fit map W20_J140_MB_joined in map W20_J3623_consensus_structure_sharp.mrc |
6597 | | using 130669 points |
6598 | | correlation = 0.3924, correlation about mean = 0.006354, overlap = 259.4 |
6599 | | steps = 144, shift = 7.92, angle = 8.21 degrees |
6600 | | |
6601 | | Position of W20_J140_MB_joined (#42) relative to |
6602 | | W20_J3623_consensus_structure_sharp.mrc (#1) coordinates: |
6603 | | Matrix rotation and translation |
6604 | | 0.91620955 0.25591004 -0.30833442 2.94589670 |
6605 | | -0.21484448 0.96327207 0.16108624 14.27677310 |
6606 | | 0.33823352 -0.08134481 0.93753993 -22.28584079 |
6607 | | Axis -0.29008545 -0.77366308 -0.56329022 |
6608 | | Axis point 63.49232840 0.00000000 -7.08518329 |
6609 | | Rotation angle (degrees) 24.69964443 |
6610 | | Shift along axis 0.65342200 |
6611 | | |
6612 | | |
6613 | | > fitmap #43 inMap #1 |
6614 | | |
6615 | | Fit map W20_J145_MB_joined in map W20_J3623_consensus_structure_sharp.mrc |
6616 | | using 142902 points |
6617 | | correlation = 0.4199, correlation about mean = 0.04049, overlap = 303 |
6618 | | steps = 164, shift = 1.19, angle = 6.55 degrees |
6619 | | |
6620 | | Position of W20_J145_MB_joined (#43) relative to |
6621 | | W20_J3623_consensus_structure_sharp.mrc (#1) coordinates: |
6622 | | Matrix rotation and translation |
6623 | | 0.99583148 0.04008635 0.08193132 -23.34504583 |
6624 | | -0.04315588 0.99841798 0.03604305 -2.05514686 |
6625 | | -0.08035687 -0.03942862 0.99598602 16.93949740 |
6626 | | Axis -0.38234856 0.82217147 -0.42171514 |
6627 | | Axis point 196.57839852 0.00000000 294.50364776 |
6628 | | Rotation angle (degrees) 5.66401964 |
6629 | | Shift along axis 0.09261895 |
6630 | | |
6631 | | |
6632 | | > fitmap #44 inMap #1 |
6633 | | |
6634 | | Fit map W20_J150_MB_joined in map W20_J3623_consensus_structure_sharp.mrc |
6635 | | using 100519 points |
6636 | | correlation = 0.4322, correlation about mean = -0.01831, overlap = 243.2 |
6637 | | steps = 80, shift = 5.66, angle = 2.97 degrees |
6638 | | |
6639 | | Position of W20_J150_MB_joined (#44) relative to |
6640 | | W20_J3623_consensus_structure_sharp.mrc (#1) coordinates: |
6641 | | Matrix rotation and translation |
6642 | | 0.99916201 0.02809111 0.02976842 -4.49636094 |
6643 | | -0.03208054 0.98915209 0.14334916 -11.35419842 |
6644 | | -0.02541866 -0.14418402 0.98922437 24.23768179 |
6645 | | Axis -0.96196921 0.18463355 -0.20130992 |
6646 | | Axis point 0.00000000 157.84496089 87.73789734 |
6647 | | Rotation angle (degrees) 8.59507208 |
6648 | | Shift along axis -2.65029098 |
6649 | | |
6650 | | |
6651 | | > fitmap #45 inMap #1 |
6652 | | |
6653 | | Fit map W20_J155_MB_joined in map W20_J3623_consensus_structure_sharp.mrc |
6654 | | using 138129 points |
6655 | | correlation = 0.4135, correlation about mean = 0.03029, overlap = 293.3 |
6656 | | steps = 136, shift = 5.25, angle = 4.6 degrees |
6657 | | |
6658 | | Position of W20_J155_MB_joined (#45) relative to |
6659 | | W20_J3623_consensus_structure_sharp.mrc (#1) coordinates: |
6660 | | Matrix rotation and translation |
6661 | | 0.97166385 -0.22776257 -0.06319474 54.87908166 |
6662 | | 0.22867640 0.97347338 0.00752908 -17.23256599 |
6663 | | 0.05980356 -0.02176688 0.99797281 -6.95123433 |
6664 | | Axis -0.06185438 -0.25969395 0.96370799 |
6665 | | Axis point 107.95346859 225.99227664 0.00000000 |
6666 | | Rotation angle (degrees) 13.69857239 |
6667 | | Shift along axis -5.61827826 |
6668 | | |
6669 | | |
6670 | | > fitmap #46 inMap #1 |
6671 | | |
6672 | | Fit map W20_J160_MB_joined in map W20_J3623_consensus_structure_sharp.mrc |
6673 | | using 121938 points |
6674 | | correlation = 0.4485, correlation about mean = 0.04548, overlap = 303.4 |
6675 | | steps = 176, shift = 10.8, angle = 12.9 degrees |
6676 | | |
6677 | | Position of W20_J160_MB_joined (#46) relative to |
6678 | | W20_J3623_consensus_structure_sharp.mrc (#1) coordinates: |
6679 | | Matrix rotation and translation |
6680 | | 0.98515785 -0.09819617 0.14078890 -1.18436719 |
6681 | | 0.06853050 0.97700616 0.20189734 -32.23116044 |
6682 | | -0.15737717 -0.18925241 0.96923472 51.60758570 |
6683 | | Axis -0.75318811 0.57414108 0.32104469 |
6684 | | Axis point 0.00000000 244.46953938 184.51242914 |
6685 | | Rotation angle (degrees) 15.05005024 |
6686 | | Shift along axis -1.04484054 |
6687 | | |
6688 | | |
6689 | | > show #!1 models |
6690 | | |
6691 | | > hide #!1 models |
6692 | | |
6693 | | > show #!2 models |
6694 | | |
6695 | | > hide #!2 models |
6696 | | |
6697 | | > hide #!42 models |
6698 | | |
6699 | | > hide #!43 models |
6700 | | |
6701 | | > hide #!44 models |
6702 | | |
6703 | | > hide #!45 models |
6704 | | |
6705 | | > hide #!46 models |
6706 | | |
6707 | | > show #!1 models |
6708 | | |
6709 | | > show #!2 models |
6710 | | |
6711 | | > hide #!2 models |
6712 | | |
6713 | | > show #!3 models |
6714 | | |
6715 | | > hide #!3 models |
6716 | | |
6717 | | > show #!4 models |
6718 | | |
6719 | | > hide #!4 models |
6720 | | |
6721 | | > show #!5 models |
6722 | | |
6723 | | > hide #!5 models |
6724 | | |
6725 | | > show #!6 models |
6726 | | |
6727 | | > hide #!6 models |
6728 | | |
6729 | | > show #!7 models |
6730 | | |
6731 | | > hide #!7 models |
6732 | | |
6733 | | > show #!8 models |
6734 | | |
6735 | | > hide #!8 models |
6736 | | |
6737 | | > show #!9 models |
6738 | | |
6739 | | > hide #!9 models |
6740 | | |
6741 | | > show #!10 models |
6742 | | |
6743 | | > hide #!10 models |
6744 | | |
6745 | | > show #!11 models |
6746 | | |
6747 | | > hide #!11 models |
6748 | | |
6749 | | > show #!12 models |
6750 | | |
6751 | | > hide #!12 models |
6752 | | |
6753 | | > show #!13 models |
6754 | | |
6755 | | > hide #!13 models |
6756 | | |
6757 | | > show #!14 models |
6758 | | |
6759 | | > hide #!14 models |
6760 | | |
6761 | | > show #!15 models |
6762 | | |
6763 | | > hide #!15 models |
6764 | | |
6765 | | > show #!16 models |
6766 | | |
6767 | | > hide #!16 models |
6768 | | |
6769 | | > show #!17 models |
6770 | | |
6771 | | > hide #!17 models |
6772 | | |
6773 | | > show #!18 models |
6774 | | |
6775 | | > hide #!18 models |
6776 | | |
6777 | | > show #!19 models |
6778 | | |
6779 | | > hide #!19 models |
6780 | | |
6781 | | > show #!20 models |
6782 | | |
6783 | | > hide #!20 models |
6784 | | |
6785 | | > show #!21 models |
6786 | | |
6787 | | > hide #!21 models |
6788 | | |
6789 | | > show #!22 models |
6790 | | |
6791 | | > hide #!22 models |
6792 | | |
6793 | | > show #!23 models |
6794 | | |
6795 | | > hide #!23 models |
6796 | | |
6797 | | > show #!24 models |
6798 | | |
6799 | | > hide #!24 models |
6800 | | |
6801 | | > show #!25 models |
6802 | | |
6803 | | > hide #!25 models |
6804 | | |
6805 | | > show #!25 models |
6806 | | |
6807 | | > color #25 silver models |
6808 | | |
6809 | | [Repeated 1 time(s)] |
6810 | | |
6811 | | > hide #!25 models |
6812 | | |
6813 | | > show #!26 models |
6814 | | |
6815 | | > hide #!26 models |
6816 | | |
6817 | | > show #!27 models |
6818 | | |
6819 | | > hide #!27 models |
6820 | | |
6821 | | > show #!28 models |
6822 | | |
6823 | | > hide #!28 models |
6824 | | |
6825 | | > show #!29 models |
6826 | | |
6827 | | > hide #!29 models |
6828 | | |
6829 | | > hide #!30 models |
6830 | | |
6831 | | > show #!30 models |
6832 | | |
6833 | | > hide #!30 models |
6834 | | |
6835 | | > show #!29 models |
6836 | | |
6837 | | > hide #!29 models |
6838 | | |
6839 | | > show #!30 models |
6840 | | |
6841 | | > hide #!30 models |
6842 | | |
6843 | | > show #!30.1 models |
6844 | | |
6845 | | > show #!30.2 models |
6846 | | |
6847 | | > show #!30.3 models |
6848 | | |
6849 | | > show #!30.4 models |
6850 | | |
6851 | | > show #!29 models |
6852 | | |
6853 | | > hide #!29 models |
6854 | | |
6855 | | > hide #!30 models |
6856 | | |
6857 | | > show #!30 models |
6858 | | |
6859 | | > hide #!30 models |
6860 | | |
6861 | | > show #!30 models |
6862 | | |
6863 | | > hide #!30 models |
6864 | | |
6865 | | > show #!30 models |
6866 | | |
6867 | | > hide #!30 models |
6868 | | |
6869 | | > show #!29.1 models |
6870 | | |
6871 | | > show #!29.2 models |
6872 | | |
6873 | | > show #!29.3 models |
6874 | | |
6875 | | > show #!29.4 models |
6876 | | |
6877 | | > hide #!29 models |
6878 | | |
6879 | | > show #!31 models |
6880 | | |
6881 | | > show #!31.1 models |
6882 | | |
6883 | | > show #!31.2 models |
6884 | | |
6885 | | > show #!31.3 models |
6886 | | |
6887 | | > show #!31.4 models |
6888 | | |
6889 | | > hide #!31 models |
6890 | | |
6891 | | > show #!31 models |
6892 | | |
6893 | | > hide #!31 models |
6894 | | |
6895 | | > show #!31 models |
6896 | | |
6897 | | > hide #!31 models |
6898 | | |
6899 | | > show #!32 models |
6900 | | |
6901 | | > show #!32.1 models |
6902 | | |
6903 | | > show #!32.2 models |
6904 | | |
6905 | | > show #!32.3 models |
6906 | | |
6907 | | > show #!32.4 models |
6908 | | |
6909 | | > hide #!32 models |
6910 | | |
6911 | | > select add #32 |
6912 | | |
6913 | | 9 models selected |
6914 | | |
6915 | | > select subtract #32 |
6916 | | |
6917 | | Nothing selected |
6918 | | |
6919 | | > show #!32 models |
6920 | | |
6921 | | > hide #!32 models |
6922 | | |
6923 | | > show #!33 models |
6924 | | |
6925 | | > show #!33.1 models |
6926 | | |
6927 | | > show #!33.2 models |
6928 | | |
6929 | | > show #!33.3 models |
6930 | | |
6931 | | > show #!33.4 models |
6932 | | |
6933 | | > hide #!33 models |
6934 | | |
6935 | | > show #!32 models |
6936 | | |
6937 | | > hide #!32 models |
6938 | | |
6939 | | > show #!33 models |
6940 | | |
6941 | | > hide #!33 models |
6942 | | |
6943 | | > show #!42 models |
6944 | | |
6945 | | > hide #!42 models |
6946 | | |
6947 | | > show #!42 models |
6948 | | |
6949 | | > show #!43 models |
6950 | | |
6951 | | > hide #!43 models |
6952 | | |
6953 | | > select add #42 |
6954 | | |
6955 | | 2 models selected |
6956 | | |
6957 | | > view matrix models |
6958 | | > #42,0.91621,0.25591,-0.30833,6.5795,-0.21484,0.96327,0.16109,11.618,0.33823,-0.081345,0.93754,-14.738 |
6959 | | |
6960 | | > ui mousemode right "rotate selected models" |
6961 | | |
6962 | | > view matrix models |
6963 | | > #42,-0.20075,-0.93591,0.28944,250.09,-0.75521,0.33604,0.5628,121.07,-0.62399,-0.10561,-0.77426,360.31 |
6964 | | |
6965 | | > ui mousemode right "translate selected models" |
6966 | | |
6967 | | > view matrix models |
6968 | | > #42,-0.20075,-0.93591,0.28944,258.45,-0.75521,0.33604,0.5628,111.09,-0.62399,-0.10561,-0.77426,373.71 |
6969 | | |
6970 | | > ui mousemode right "rotate selected models" |
6971 | | |
6972 | | > view matrix models |
6973 | | > #42,-0.33018,-0.9414,-0.068949,327.26,-0.81305,0.24654,0.52742,137.15,-0.47951,0.2302,-0.8468,314.67 |
6974 | | |
6975 | | > fitmap #42 inMap #1 |
6976 | | |
6977 | | Fit map W20_J140_MB_joined in map W20_J3623_consensus_structure_sharp.mrc |
6978 | | using 130669 points |
6979 | | correlation = 0.8803, correlation about mean = 0.3684, overlap = 1189 |
6980 | | steps = 172, shift = 19.9, angle = 7.77 degrees |
6981 | | |
6982 | | Position of W20_J140_MB_joined (#42) relative to |
6983 | | W20_J3623_consensus_structure_sharp.mrc (#1) coordinates: |
6984 | | Matrix rotation and translation |
6985 | | -0.35155267 -0.92578085 -0.13907028 347.82271473 |
6986 | | -0.76197836 0.19666262 0.61701928 136.35497869 |
6987 | | -0.54387471 0.32288331 -0.77456224 286.91060622 |
6988 | | Axis -0.55865111 0.76884323 0.31110933 |
6989 | | Axis point 248.45663259 0.00000000 85.56946066 |
6990 | | Rotation angle (degrees) 164.73667959 |
6991 | | Shift along axis -0.21537821 |
6992 | | |
6993 | | |
6994 | | > select subtract #42 |
6995 | | |
6996 | | Nothing selected |
6997 | | |
6998 | | > hide #!42 models |
6999 | | |
7000 | | > show #!43 models |
7001 | | |
7002 | | > select add #43 |
7003 | | |
7004 | | 2 models selected |
7005 | | |
7006 | | > view matrix models |
7007 | | > #43,-0.71667,-0.59905,-0.3571,362.32,-0.66464,0.43155,0.60993,81.644,-0.21127,0.67446,-0.70744,174.56 |
7008 | | |
7009 | | > view matrix models |
7010 | | > #43,-0.22267,-0.96408,0.14484,277.38,-0.843,0.26503,0.46809,150.16,-0.48966,-0.017866,-0.87173,336.37 |
7011 | | |
7012 | | > ui mousemode right "translate selected models" |
7013 | | |
7014 | | > view matrix models |
7015 | | > #43,-0.22267,-0.96408,0.14484,278.5,-0.843,0.26503,0.46809,152.18,-0.48966,-0.017866,-0.87173,341.6 |
7016 | | |
7017 | | > fitmap #43 inMap #1 |
7018 | | |
7019 | | Fit map W20_J145_MB_joined in map W20_J3623_consensus_structure_sharp.mrc |
7020 | | using 142902 points |
7021 | | correlation = 0.8839, correlation about mean = 0.3912, overlap = 1241 |
7022 | | steps = 176, shift = 16.1, angle = 20.5 degrees |
7023 | | |
7024 | | Position of W20_J145_MB_joined (#43) relative to |
7025 | | W20_J3623_consensus_structure_sharp.mrc (#1) coordinates: |
7026 | | Matrix rotation and translation |
7027 | | -0.33273365 -0.93244330 -0.14084678 345.90650730 |
7028 | | -0.76625576 0.18027360 0.61672809 139.19380919 |
7029 | | -0.54967302 0.31313085 -0.77447314 289.28503932 |
7030 | | Axis -0.56677541 0.76322385 0.31024988 |
7031 | | Axis point 250.48919740 0.00000000 85.44391549 |
7032 | | Rotation angle (degrees) 164.46490776 |
7033 | | Shift along axis -0.06461673 |
7034 | | |
7035 | | |
7036 | | > select subtract #43 |
7037 | | |
7038 | | Nothing selected |
7039 | | |
7040 | | > hide #!43 models |
7041 | | |
7042 | | > show #!44 models |
7043 | | |
7044 | | > select add #44 |
7045 | | |
7046 | | 2 models selected |
7047 | | |
7048 | | > ui mousemode right "rotate selected models" |
7049 | | |
7050 | | > view matrix models |
7051 | | > #44,-0.64334,-0.25066,-0.72339,371.34,-0.44115,0.89362,0.082686,67.718,0.62571,0.37232,-0.68547,101.18 |
7052 | | |
7053 | | > view matrix models |
7054 | | > #44,-0.33974,-0.91968,0.1969,294.7,-0.6214,0.37665,0.68702,81.003,-0.706,0.11106,-0.69945,324.73 |
7055 | | |
7056 | | > view matrix models |
7057 | | > #44,-0.34685,-0.93744,0.029929,322.46,-0.77694,0.30504,0.55074,132.64,-0.52542,0.16777,-0.83414,311.06 |
7058 | | |
7059 | | > ui mousemode right "translate selected models" |
7060 | | |
7061 | | > view matrix models |
7062 | | > #44,-0.34685,-0.93744,0.029929,328.6,-0.77694,0.30504,0.55074,126.41,-0.52542,0.16777,-0.83414,322.03 |
7063 | | |
7064 | | > fitmap #44 inMap #1 |
7065 | | |
7066 | | Fit map W20_J150_MB_joined in map W20_J3623_consensus_structure_sharp.mrc |
7067 | | using 100519 points |
7068 | | correlation = 0.8806, correlation about mean = 0.2563, overlap = 1060 |
7069 | | steps = 104, shift = 9.91, angle = 11.1 degrees |
7070 | | |
7071 | | Position of W20_J150_MB_joined (#44) relative to |
7072 | | W20_J3623_consensus_structure_sharp.mrc (#1) coordinates: |
7073 | | Matrix rotation and translation |
7074 | | -0.34988200 -0.92650940 -0.13843016 347.22501351 |
7075 | | -0.75857847 0.19350448 0.62218544 135.60290867 |
7076 | | -0.54967381 0.32270162 -0.77053382 287.77852170 |
7077 | | Axis -0.55901416 0.76762419 0.31345858 |
7078 | | Axis point 248.55561653 0.00000000 85.33747140 |
7079 | | Rotation angle (degrees) 164.46257054 |
7080 | | Shift along axis 0.19501983 |
7081 | | |
7082 | | |
7083 | | > select subtract #44 |
7084 | | |
7085 | | Nothing selected |
7086 | | |
7087 | | > hide #!44 models |
7088 | | |
7089 | | > show #!45 models |
7090 | | |
7091 | | > ui mousemode right "rotate selected models" |
7092 | | |
7093 | | > select add #45 |
7094 | | |
7095 | | 2 models selected |
7096 | | |
7097 | | > view matrix models |
7098 | | > #45,-0.93945,-0.28966,-0.18313,341.27,-0.33735,0.87568,0.34552,26.538,0.060286,0.38637,-0.92037,203.81 |
7099 | | |
7100 | | > view matrix models |
7101 | | > #45,-0.39594,-0.91199,0.10724,318.12,-0.72625,0.38247,0.57121,120.46,-0.56195,0.14828,-0.81377,308.66 |
7102 | | |
7103 | | > fitmap #45 inMap #1 |
7104 | | |
7105 | | Fit map W20_J155_MB_joined in map W20_J3623_consensus_structure_sharp.mrc |
7106 | | using 138129 points |
7107 | | correlation = 0.8776, correlation about mean = 0.3767, overlap = 1250 |
7108 | | steps = 140, shift = 15.4, angle = 15.9 degrees |
7109 | | |
7110 | | Position of W20_J155_MB_joined (#45) relative to |
7111 | | W20_J3623_consensus_structure_sharp.mrc (#1) coordinates: |
7112 | | Matrix rotation and translation |
7113 | | -0.35675611 -0.91947462 -0.16520144 351.40689174 |
7114 | | -0.78155048 0.19688467 0.59195886 143.25471986 |
7115 | | -0.51176551 0.34029820 -0.78885563 282.02499467 |
7116 | | Axis -0.55929726 0.77021311 0.30652624 |
7117 | | Axis point 248.57307844 0.00000000 86.50174383 |
7118 | | Rotation angle (degrees) 166.99833687 |
7119 | | Shift along axis 0.24381166 |
7120 | | |
7121 | | |
7122 | | > select subtract #45 |
7123 | | |
7124 | | Nothing selected |
7125 | | |
7126 | | > hide #!45 models |
7127 | | |
7128 | | > show #!46 models |
7129 | | |
7130 | | > select add #46 |
7131 | | |
7132 | | 2 models selected |
7133 | | |
7134 | | > view matrix models |
7135 | | > #46,-0.25068,-0.94965,-0.18794,340.4,-0.93649,0.1887,0.29561,209.1,-0.24527,0.25011,-0.93664,265.98 |
7136 | | |
7137 | | > view matrix models |
7138 | | > #46,-0.071284,-0.98257,0.17165,270.25,-0.5959,0.17996,0.78264,95.4,-0.79989,-0.046498,-0.59834,338.43 |
7139 | | |
7140 | | > ui mousemode right "translate selected models" |
7141 | | |
7142 | | > view matrix models |
7143 | | > #46,-0.071284,-0.98257,0.17165,275.5,-0.5959,0.17996,0.78264,90.643,-0.79989,-0.046498,-0.59834,355.66 |
7144 | | |
7145 | | > view matrix models |
7146 | | > #46,-0.071284,-0.98257,0.17165,285.03,-0.5959,0.17996,0.78264,91.932,-0.79989,-0.046498,-0.59834,362.22 |
7147 | | |
7148 | | > fitmap #46 inMap #1 |
7149 | | |
7150 | | Fit map W20_J160_MB_joined in map W20_J3623_consensus_structure_sharp.mrc |
7151 | | using 121938 points |
7152 | | correlation = 0.8667, correlation about mean = 0.3087, overlap = 1157 |
7153 | | steps = 208, shift = 18.2, angle = 31.4 degrees |
7154 | | |
7155 | | Position of W20_J160_MB_joined (#46) relative to |
7156 | | W20_J3623_consensus_structure_sharp.mrc (#1) coordinates: |
7157 | | Matrix rotation and translation |
7158 | | -0.39178850 -0.90130925 -0.18477932 357.17449457 |
7159 | | -0.76159854 0.20502417 0.61476235 135.64467847 |
7160 | | -0.51620677 0.38158448 -0.76676193 274.65181077 |
7161 | | Axis -0.54399036 0.77320089 0.32593693 |
7162 | | Axis point 245.55803639 0.00000000 83.69005707 |
7163 | | Rotation angle (degrees) 167.62426420 |
7164 | | Shift along axis 0.10027404 |
7165 | | |
7166 | | |
7167 | | > select subtract #46 |
7168 | | |
7169 | | Nothing selected |
7170 | | |
7171 | | > hide #!46 models |
7172 | | |
7173 | | > show #!46 models |
7174 | | |
7175 | | > hide #!46 models |
7176 | | |
7177 | | > save "/Users/becca/Desktop/Postdoc/COPI notebooks and |
7178 | | > resources/Processing/W20_MM_cyto_COPI/20250701_W20_COPI_cyto_alignments.cxs" |
7179 | | |
7180 | | ——— End of log from Wed Jul 2 15:03:33 2025 ——— |
7181 | | |
7182 | | > view name session-start |
7183 | | |
7184 | | opened ChimeraX session |
7185 | | |
7186 | | > hide #!1 models |
7187 | | |
7188 | | > show #!46 models |
7189 | | |
7190 | | > show #!45 models |
7191 | | |
7192 | | > show #!25 models |
7193 | | |
7194 | | > color #25 #ff2600ff models |
7195 | | |
7196 | | > color #46 #ff9300ff models |
7197 | | |
7198 | | > color #45 #fffb00ff models |
7199 | | |
7200 | | > show #!9 models |
7201 | | |
7202 | | > color #9 #8efa00ff models |
7203 | | |
7204 | | > show #!27 models |
7205 | | |
7206 | | > color #27 #00fdffff models |
7207 | | |
7208 | | > show #!23 models |
7209 | | |
7210 | | > color #23 #0433ffff models |
7211 | | |
7212 | | > hide #!27 models |
7213 | | |
7214 | | > show #!27 models |
7215 | | |
7216 | | > hide #!27 models |
7217 | | |
7218 | | > hide #!25 models |
7219 | | |
7220 | | > hide #!23 models |
7221 | | |
7222 | | > hide #!9 models |
7223 | | |
7224 | | > hide #!45 models |
7225 | | |
7226 | | > hide #!46 models |
7227 | | |
7228 | | > show #!46 models |
7229 | | |
7230 | | > show #!23 models |
7231 | | |
7232 | | > hide #!46 models |
7233 | | |
7234 | | > hide #!23 models |
7235 | | |
7236 | | > show #!46 models |
7237 | | |
7238 | | > hide #!1 models |
7239 | | |
7240 | | > save "/Users/becca/Desktop/Postdoc/COPI notebooks and |
7241 | | > resources/Processing/W20_MM_cyto_COPI/20250701_W20_COPI_cyto_alignments.cxs" |
7242 | | |
7243 | | ——— End of log from Wed Jul 2 17:43:06 2025 ——— |
7244 | | |
7245 | | opened ChimeraX session |
7246 | | |
7247 | | > color list |
7248 | | |
7249 | | 28 custom colors: alphav1 |
7250 | | |
7251 | | , alphav2 |
7252 | | |
7253 | | , alphav3 |
7254 | | |
7255 | | , arf1v1 |
7256 | | |
7257 | | , arf1v2 |
7258 | | |
7259 | | , arf1v3 |
7260 | | |
7261 | | , betapv1 |
7262 | | |
7263 | | , betapv2 |
7264 | | |
7265 | | , betapv3 |
7266 | | |
7267 | | , betav1 |
7268 | | |
7269 | | , betav2 |
7270 | | |
7271 | | , betav3 |
7272 | | |
7273 | | , deltav1 |
7274 | | |
7275 | | , deltav2 |
7276 | | |
7277 | | , deltav3 |
7278 | | |
7279 | | , epsilonv1 |
7280 | | |
7281 | | , epsilonv3 |
7282 | | |
7283 | | , gammav1 |
7284 | | |
7285 | | , gammav2 |
7286 | | |
7287 | | , gammav3 |
7288 | | |
7289 | | , label_purple |
7290 | | |
7291 | | , label_purple_v3 |
7292 | | |
7293 | | , label_red |
7294 | | |
7295 | | , label_red_v3 |
7296 | | |
7297 | | , lable_purple_v4 |
7298 | | |
7299 | | , zetav1 |
7300 | | |
7301 | | , zetav2 |
7302 | | |
7303 | | , and zetav3 |
7304 | | |
7305 | | . |
7306 | | 248 builtin colors: alice blue |
7307 | | |
7308 | | , aliceblue |
7309 | | |
7310 | | , antique white |
7311 | | |
7312 | | , antiquewhite |
7313 | | |
7314 | | , aqua |
7315 | | |
7316 | | , aquamarine |
7317 | | |
7318 | | , azure |
7319 | | |
7320 | | , beige |
7321 | | |
7322 | | , bisque |
7323 | | |
7324 | | , black |
7325 | | |
7326 | | , blanched almond |
7327 | | |
7328 | | , blanchedalmond |
7329 | | |
7330 | | , blue |
7331 | | |
7332 | | , blue violet |
7333 | | |
7334 | | , blueviolet |
7335 | | |
7336 | | , brown |
7337 | | |
7338 | | , burly wood |
7339 | | |
7340 | | , burlywood |
7341 | | |
7342 | | , cadet blue |
7343 | | |
7344 | | , cadetblue |
7345 | | |
7346 | | , chartreuse |
7347 | | |
7348 | | , chocolate |
7349 | | |
7350 | | , coral |
7351 | | |
7352 | | , cornflower blue |
7353 | | |
7354 | | , cornflowerblue |
7355 | | |
7356 | | , cornsilk |
7357 | | |
7358 | | , crimson |
7359 | | |
7360 | | , cyan |
7361 | | |
7362 | | , dark blue |
7363 | | |
7364 | | , dark cyan |
7365 | | |
7366 | | , dark goldenrod |
7367 | | |
7368 | | , dark gray |
7369 | | |
7370 | | , dark green |
7371 | | |
7372 | | , dark grey |
7373 | | |
7374 | | , dark khaki |
7375 | | |
7376 | | , dark magenta |
7377 | | |
7378 | | , dark olive green |
7379 | | |
7380 | | , dark orange |
7381 | | |
7382 | | , dark orchid |
7383 | | |
7384 | | , dark red |
7385 | | |
7386 | | , dark salmon |
7387 | | |
7388 | | , dark sea green |
7389 | | |
7390 | | , dark seagreen |
7391 | | |
7392 | | , dark slate blue |
7393 | | |
7394 | | , dark slate gray |
7395 | | |
7396 | | , dark slate grey |
7397 | | |
7398 | | , dark turquoise |
7399 | | |
7400 | | , dark violet |
7401 | | |
7402 | | , darkblue |
7403 | | |
7404 | | , darkcyan |
7405 | | |
7406 | | , darkgoldenrod |
7407 | | |
7408 | | , darkgray |
7409 | | |
7410 | | , darkgreen |
7411 | | |
7412 | | , darkgrey |
7413 | | |
7414 | | , darkkhaki |
7415 | | |
7416 | | , darkmagenta |
7417 | | |
7418 | | , darkolivegreen |
7419 | | |
7420 | | , darkorange |
7421 | | |
7422 | | , darkorchid |
7423 | | |
7424 | | , darkred |
7425 | | |
7426 | | , darksalmon |
7427 | | |
7428 | | , darkseagreen |
7429 | | |
7430 | | , darkslateblue |
7431 | | |
7432 | | , darkslategray |
7433 | | |
7434 | | , darkslategrey |
7435 | | |
7436 | | , darkturquoise |
7437 | | |
7438 | | , darkviolet |
7439 | | |
7440 | | , deep pink |
7441 | | |
7442 | | , deep sky blue |
7443 | | |
7444 | | , deep skyblue |
7445 | | |
7446 | | , deeppink |
7447 | | |
7448 | | , deepskyblue |
7449 | | |
7450 | | , dim gray |
7451 | | |
7452 | | , dim grey |
7453 | | |
7454 | | , dimgray |
7455 | | |
7456 | | , dimgrey |
7457 | | |
7458 | | , dodger blue |
7459 | | |
7460 | | , dodgerblue |
7461 | | |
7462 | | , fire brick |
7463 | | |
7464 | | , firebrick |
7465 | | |
7466 | | , floral white |
7467 | | |
7468 | | , floralwhite |
7469 | | |
7470 | | , forest green |
7471 | | |
7472 | | , forestgreen |
7473 | | |
7474 | | , fuchsia |
7475 | | |
7476 | | , gainsboro |
7477 | | |
7478 | | , ghost white |
7479 | | |
7480 | | , ghostwhite |
7481 | | |
7482 | | , gold |
7483 | | |
7484 | | , goldenrod |
7485 | | |
7486 | | , gray |
7487 | | |
7488 | | , green |
7489 | | |
7490 | | , green yellow |
7491 | | |
7492 | | , greenyellow |
7493 | | |
7494 | | , grey |
7495 | | |
7496 | | , honeydew |
7497 | | |
7498 | | , hot pink |
7499 | | |
7500 | | , hotpink |
7501 | | |
7502 | | , indian red |
7503 | | |
7504 | | , indianred |
7505 | | |
7506 | | , indigo |
7507 | | |
7508 | | , ivory |
7509 | | |
7510 | | , khaki |
7511 | | |
7512 | | , lavender |
7513 | | |
7514 | | , lavender blush |
7515 | | |
7516 | | , lavenderblush |
7517 | | |
7518 | | , lawn green |
7519 | | |
7520 | | , lawngreen |
7521 | | |
7522 | | , lemon chiffon |
7523 | | |
7524 | | , lemonchiffon |
7525 | | |
7526 | | , light blue |
7527 | | |
7528 | | , light coral |
7529 | | |
7530 | | , light cyan |
7531 | | |
7532 | | , light goldenrod yellow |
7533 | | |
7534 | | , light gray |
7535 | | |
7536 | | , light green |
7537 | | |
7538 | | , light grey |
7539 | | |
7540 | | , light pink |
7541 | | |
7542 | | , light salmon |
7543 | | |
7544 | | , light sea green |
7545 | | |
7546 | | , light seagreen |
7547 | | |
7548 | | , light sky blue |
7549 | | |
7550 | | , light skyblue |
7551 | | |
7552 | | , light slate gray |
7553 | | |
7554 | | , light slate grey |
7555 | | |
7556 | | , light steel blue |
7557 | | |
7558 | | , light yellow |
7559 | | |
7560 | | , lightblue |
7561 | | |
7562 | | , lightcoral |
7563 | | |
7564 | | , lightcyan |
7565 | | |
7566 | | , lightgoldenrodyellow |
7567 | | |
7568 | | , lightgray |
7569 | | |
7570 | | , lightgreen |
7571 | | |
7572 | | , lightgrey |
7573 | | |
7574 | | , lightpink |
7575 | | |
7576 | | , lightsalmon |
7577 | | |
7578 | | , lightseagreen |
7579 | | |
7580 | | , lightskyblue |
7581 | | |
7582 | | , lightslategray |
7583 | | |
7584 | | , lightslategrey |
7585 | | |
7586 | | , lightsteelblue |
7587 | | |
7588 | | , lightyellow |
7589 | | |
7590 | | , lime |
7591 | | |
7592 | | , lime green |
7593 | | |
7594 | | , limegreen |
7595 | | |
7596 | | , linen |
7597 | | |
7598 | | , magenta |
7599 | | |
7600 | | , maroon |
7601 | | |
7602 | | , medium aquamarine |
7603 | | |
7604 | | , medium blue |
7605 | | |
7606 | | , medium orchid |
7607 | | |
7608 | | , medium purple |
7609 | | |
7610 | | , medium sea green |
7611 | | |
7612 | | , medium seagreen |
7613 | | |
7614 | | , medium slate blue |
7615 | | |
7616 | | , medium spring green |
7617 | | |
7618 | | , medium turquoise |
7619 | | |
7620 | | , medium violet red |
7621 | | |
7622 | | , mediumaquamarine |
7623 | | |
7624 | | , mediumblue |
7625 | | |
7626 | | , mediumorchid |
7627 | | |
7628 | | , mediumpurple |
7629 | | |
7630 | | , mediumseagreen |
7631 | | |
7632 | | , mediumslateblue |
7633 | | |
7634 | | , mediumspringgreen |
7635 | | |
7636 | | , mediumturquoise |
7637 | | |
7638 | | , mediumvioletred |
7639 | | |
7640 | | , midnight blue |
7641 | | |
7642 | | , midnightblue |
7643 | | |
7644 | | , mint cream |
7645 | | |
7646 | | , mintcream |
7647 | | |
7648 | | , misty rose |
7649 | | |
7650 | | , mistyrose |
7651 | | |
7652 | | , moccasin |
7653 | | |
7654 | | , navajo white |
7655 | | |
7656 | | , navajowhite |
7657 | | |
7658 | | , navy |
7659 | | |
7660 | | , old lace |
7661 | | |
7662 | | , oldlace |
7663 | | |
7664 | | , olive |
7665 | | |
7666 | | , olive drab |
7667 | | |
7668 | | , olivedrab |
7669 | | |
7670 | | , orange |
7671 | | |
7672 | | , orange red |
7673 | | |
7674 | | , orangered |
7675 | | |
7676 | | , orchid |
7677 | | |
7678 | | , pale goldenrod |
7679 | | |
7680 | | , pale green |
7681 | | |
7682 | | , pale turquoise |
7683 | | |
7684 | | , pale violet red |
7685 | | |
7686 | | , palegoldenrod |
7687 | | |
7688 | | , palegreen |
7689 | | |
7690 | | , paleturquoise |
7691 | | |
7692 | | , palevioletred |
7693 | | |
7694 | | , papaya whip |
7695 | | |
7696 | | , papayawhip |
7697 | | |
7698 | | , peach puff |
7699 | | |
7700 | | , peachpuff |
7701 | | |
7702 | | , peru |
7703 | | |
7704 | | , pink |
7705 | | |
7706 | | , plum |
7707 | | |
7708 | | , powder blue |
7709 | | |
7710 | | , powderblue |
7711 | | |
7712 | | , purple |
7713 | | |
7714 | | , rebecca purple |
7715 | | |
7716 | | , rebeccapurple |
7717 | | |
7718 | | , red |
7719 | | |
7720 | | , rosy brown |
7721 | | |
7722 | | , rosybrown |
7723 | | |
7724 | | , royal blue |
7725 | | |
7726 | | , royalblue |
7727 | | |
7728 | | , saddle brown |
7729 | | |
7730 | | , saddlebrown |
7731 | | |
7732 | | , salmon |
7733 | | |
7734 | | , sandy brown |
7735 | | |
7736 | | , sandybrown |
7737 | | |
7738 | | , sea green |
7739 | | |
7740 | | , seagreen |
7741 | | |
7742 | | , seashell |
7743 | | |
7744 | | , sienna |
7745 | | |
7746 | | , silver |
7747 | | |
7748 | | , sky blue |
7749 | | |
7750 | | , skyblue |
7751 | | |
7752 | | , slate blue |
7753 | | |
7754 | | , slate gray |
7755 | | |
7756 | | , slate grey |
7757 | | |
7758 | | , slateblue |
7759 | | |
7760 | | , slategray |
7761 | | |
7762 | | , slategrey |
7763 | | |
7764 | | , snow |
7765 | | |
7766 | | , spring green |
7767 | | |
7768 | | , springgreen |
7769 | | |
7770 | | , steel blue |
7771 | | |
7772 | | , steelblue |
7773 | | |
7774 | | , tan |
7775 | | |
7776 | | , teal |
7777 | | |
7778 | | , thistle |
7779 | | |
7780 | | , tomato |
7781 | | |
7782 | | , transparent |
7783 | | |
7784 | | , turquoise |
7785 | | |
7786 | | , violet |
7787 | | |
7788 | | , wheat |
7789 | | |
7790 | | , white |
7791 | | |
7792 | | , white smoke |
7793 | | |
7794 | | , whitesmoke |
7795 | | |
7796 | | , yellow |
7797 | | |
7798 | | , yellow green |
7799 | | |
7800 | | , and yellowgreen |
7801 | | |
7802 | | . |
7803 | | |
7804 | | > color name gamma_1 #5EAF5A |
7805 | | |
7806 | | Color 'gamma_1' is opaque: rgb(36.9%, 68.6%, 35.3%) hex: #5eaf5a |
7807 | | |
7808 | | |
7809 | | |
7810 | | > color name gamma_2 #83CA6B |
7811 | | |
7812 | | Color 'gamma_2' is opaque: rgb(51.4%, 79.2%, 42%) hex: #83ca6b |
7813 | | |
7814 | | |
7815 | | |
7816 | | > color name gamma_3 #ADE57C |
7817 | | |
7818 | | Color 'gamma_3' is opaque: rgb(67.8%, 89.8%, 48.6%) hex: #ade57c |
7819 | | |
7820 | | |
7821 | | |
7822 | | > color name gamma_4 #C9EA8C |
7823 | | |
7824 | | Color 'gamma_4' is opaque: rgb(78.8%, 91.8%, 54.9%) hex: #c9ea8c |
7825 | | |
7826 | | |
7827 | | |
7828 | | > color name gamma_5 #E1EF9C |
7829 | | |
7830 | | Color 'gamma_5' is opaque: rgb(88.2%, 93.7%, 61.2%) hex: #e1ef9c |
7831 | | |
7832 | | |
7833 | | |
7834 | | > color name gamma_6 #F3FIAD |
7835 | | |
7836 | | Missing or invalid "color" argument: expected hexadecimal digits after # |
7837 | | |
7838 | | > color name gamma_6 #F3F1AD |
7839 | | |
7840 | | Color 'gamma_6' is opaque: rgb(95.3%, 94.5%, 67.8%) hex: #f3f1ad |
7841 | | |
7842 | | |
7843 | | |
7844 | | > show #!45 models |
7845 | | |
7846 | | > hide #!45 models |
7847 | | |
7848 | | > hide #!46 models |
7849 | | |
7850 | | > show #!46 models |
7851 | | |
7852 | | > show #!45 models |
7853 | | |
7854 | | > show #!25 models |
7855 | | |
7856 | | > hide #!46 models |
7857 | | |
7858 | | > hide #!45 models |
7859 | | |
7860 | | > show #!38 models |
7861 | | |
7862 | | > show #35 models |
7863 | | |
7864 | | > color #25 silver models |
7865 | | |
7866 | | > color #25 #c0c0c074 models |
7867 | | |
7868 | | > open "/Users/becca/Desktop/Postdoc/COPI notebooks and |
7869 | | > resources/Processing/W20_MM_cyto_COPI/Icknield_workshop_files/W20_models/W20_C1_initial_model_v3.pdb" |
7870 | | |
7871 | | Chain information for W20_C1_initial_model_v3.pdb #47 |
7872 | | --- |
7873 | | Chain | Description |
7874 | | D E | No description available |
7875 | | F | No description available |
7876 | | G | No description available |
7877 | | H | No description available |
7878 | | I | No description available |
7879 | | J | No description available |
7880 | | K | No description available |
7881 | | L | No description available |
7882 | | |
7883 | | |
7884 | | > select add #47 |
7885 | | |
7886 | | 28705 atoms, 29239 bonds, 3 pseudobonds, 3615 residues, 2 models selected |
7887 | | |
7888 | | > hide sel atoms |
7889 | | |
7890 | | > show sel cartoons |
7891 | | |
7892 | | > hide #!38 models |
7893 | | |
7894 | | > hide #35 models |
7895 | | |
7896 | | > view matrix models #47,1,0,0,-79.232,0,1,0,36.623,0,0,1,9.1376 |
7897 | | |
7898 | | > view matrix models #47,1,0,0,-13.804,0,1,0,-20.445,0,0,1,10.191 |
7899 | | |
7900 | | > view matrix models #47,1,0,0,0.72332,0,1,0,-4.6589,0,0,1,-2.6371 |
7901 | | |
7902 | | > fitmap #47 inMap #1 |
7903 | | |
7904 | | Fit molecule W20_C1_initial_model_v3.pdb (#47) to map |
7905 | | W20_J3623_consensus_structure_sharp.mrc (#1) using 28705 atoms |
7906 | | average map value = 0.301, steps = 80 |
7907 | | shifted from previous position = 5.02 |
7908 | | rotated from previous position = 0.504 degrees |
7909 | | atoms outside contour = 16132, contour level = 0.26383 |
7910 | | |
7911 | | Position of W20_C1_initial_model_v3.pdb (#47) relative to |
7912 | | W20_J3623_consensus_structure_sharp.mrc (#1) coordinates: |
7913 | | Matrix rotation and translation |
7914 | | 0.99996809 -0.00526689 0.00600723 0.01730039 |
7915 | | 0.00528904 0.99997925 -0.00367710 -0.57665128 |
7916 | | -0.00598774 0.00370875 0.99997520 0.14412341 |
7917 | | Axis 0.41958417 0.68142506 0.59967410 |
7918 | | Axis point 54.13790036 0.00000000 -23.49447330 |
7919 | | Rotation angle (degrees) 0.50428904 |
7920 | | Shift along axis -0.29925859 |
7921 | | |
7922 | | |
7923 | | > select subtract #47 |
7924 | | |
7925 | | Nothing selected |
7926 | | |
7927 | | > hide #!47 models |
7928 | | |
7929 | | > show #!47 models |
7930 | | |
7931 | | > split #47 chains |
7932 | | |
7933 | | Split W20_C1_initial_model_v3.pdb (#47) into 9 models |
7934 | | Chain information for W20_C1_initial_model_v3.pdb D #47.1 |
7935 | | --- |
7936 | | Chain | Description |
7937 | | D | No description available |
7938 | | |
7939 | | Chain information for W20_C1_initial_model_v3.pdb E #47.2 |
7940 | | --- |
7941 | | Chain | Description |
7942 | | E | No description available |
7943 | | |
7944 | | Chain information for W20_C1_initial_model_v3.pdb F #47.3 |
7945 | | --- |
7946 | | Chain | Description |
7947 | | F | No description available |
7948 | | |
7949 | | Chain information for W20_C1_initial_model_v3.pdb G #47.4 |
7950 | | --- |
7951 | | Chain | Description |
7952 | | G | No description available |
7953 | | |
7954 | | Chain information for W20_C1_initial_model_v3.pdb H #47.5 |
7955 | | --- |
7956 | | Chain | Description |
7957 | | H | No description available |
7958 | | |
7959 | | Chain information for W20_C1_initial_model_v3.pdb I #47.6 |
7960 | | --- |
7961 | | Chain | Description |
7962 | | I | No description available |
7963 | | |
7964 | | Chain information for W20_C1_initial_model_v3.pdb J #47.7 |
7965 | | --- |
7966 | | Chain | Description |
7967 | | J | No description available |
7968 | | |
7969 | | Chain information for W20_C1_initial_model_v3.pdb K #47.8 |
7970 | | --- |
7971 | | Chain | Description |
7972 | | K | No description available |
7973 | | |
7974 | | Chain information for W20_C1_initial_model_v3.pdb L #47.9 |
7975 | | --- |
7976 | | Chain | Description |
7977 | | L | No description available |
7978 | | |
7979 | | |
7980 | | > save "/Users/becca/Desktop/Postdoc/COPI notebooks and |
7981 | | > resources/Processing/W20_MM_cyto_COPI/20250703_W20_COPI_cyto_alignments.cxs" |
7982 | | |
7983 | | ——— End of log from Thu Jul 3 17:41:19 2025 ——— |
7984 | | |
7985 | | opened ChimeraX session |
7986 | | |
7987 | | > open "/Users/becca/Desktop/Postdoc/COPI notebooks and |
7988 | | > resources/Processing/W20_MM_cyto_COPI/Icknield_workshop_files/W20_models/FlexFit_W20_C1_initial_model_v3/W20_C1_initial_model_v3_no_wat_lig.pdb" |
7989 | | |
7990 | | Chain information for W20_C1_initial_model_v3_no_wat_lig.pdb #48 |
7991 | | --- |
7992 | | Chain | Description |
7993 | | D E | No description available |
7994 | | F | No description available |
7995 | | G | No description available |
7996 | | H | No description available |
7997 | | I | No description available |
7998 | | J | No description available |
7999 | | K | No description available |
8000 | | L | No description available |
8001 | | |
8002 | | |
8003 | | > hide #47.1-5,9#!48#!47.6-8 atoms |
8004 | | |
8005 | | > show #47.1-5,9#!48#!47.6-8 cartoons |
8006 | | |
8007 | | > hide #!47 models |
8008 | | |
8009 | | > hide #!48 models |
8010 | | |
8011 | | > open "/Users/becca/Desktop/Postdoc/COPI notebooks and |
8012 | | > resources/Processing/W20_MM_cyto_COPI/Icknield_workshop_files/W20_models/RIBFIND_W20_C1_initial_model_v3/W20_C1_initial_model_v3_no_wat_lig.pdb" |
8013 | | |
8014 | | Chain information for W20_C1_initial_model_v3_no_wat_lig.pdb #49 |
8015 | | --- |
8016 | | Chain | Description |
8017 | | D E | No description available |
8018 | | F | No description available |
8019 | | G | No description available |
8020 | | H | No description available |
8021 | | I | No description available |
8022 | | J | No description available |
8023 | | K | No description available |
8024 | | L | No description available |
8025 | | |
8026 | | |
8027 | | > hide #!49 atoms |
8028 | | |
8029 | | > show #!49 cartoons |
8030 | | |
8031 | | > show #!48 models |
8032 | | |
8033 | | > hide #!48 models |
8034 | | |
8035 | | > show #!47 models |
8036 | | |
8037 | | > hide #!47 models |
8038 | | |
8039 | | > hide #!49 models |
8040 | | |
8041 | | > open "/Users/becca/Desktop/Postdoc/COPI notebooks and |
8042 | | > resources/Processing/W20_MM_cyto_COPI/Icknield_workshop_files/W20_models/FlexFit_W20_C1_initial_model_TEMPyReff_flexfit/W20_C1_initial_model_no_wat_lig.pdb" |
8043 | | |
8044 | | Chain information for W20_C1_initial_model_no_wat_lig.pdb #50 |
8045 | | --- |
8046 | | Chain | Description |
8047 | | A0 | No description available |
8048 | | A1 | No description available |
8049 | | A2 | No description available |
8050 | | A3 | No description available |
8051 | | A4 | No description available |
8052 | | A5 | No description available |
8053 | | A6 | No description available |
8054 | | A7 | No description available |
8055 | | A8 | No description available |
8056 | | A9 | No description available |
8057 | | BA | No description available |
8058 | | |
8059 | | |
8060 | | > hide #!50 atoms |
8061 | | |
8062 | | > show #!50 cartoons |
8063 | | |
8064 | | > select add #50 |
8065 | | |
8066 | | 25890 atoms, 26364 bonds, 22 pseudobonds, 3243 residues, 2 models selected |
8067 | | |
8068 | | > select subtract #50 |
8069 | | |
8070 | | Nothing selected |
8071 | | |
8072 | | > ui tool show Matchmaker |
8073 | | |
8074 | | > matchmaker #!50 to #34 |
8075 | | |
8076 | | Computing secondary structure |
8077 | | Parameters |
8078 | | --- |
8079 | | Chain pairing | bb |
8080 | | Alignment algorithm | Needleman-Wunsch |
8081 | | Similarity matrix | BLOSUM-62 |
8082 | | SS fraction | 0.3 |
8083 | | Gap open (HH/SS/other) | 18/18/6 |
8084 | | Gap extend | 1 |
8085 | | SS matrix | | | H | S | O |
8086 | | ---|---|---|--- |
8087 | | H | 6 | -9 | -6 |
8088 | | S | | 6 | -6 |
8089 | | O | | | 4 |
8090 | | Iteration cutoff | 2 |
8091 | | |
8092 | | Matchmaker CopA-F8WHL2.pdb, chain A (#34) with |
8093 | | W20_C1_initial_model_no_wat_lig.pdb, chain A2 (#50), sequence alignment score |
8094 | | = 2196.3 |
8095 | | RMSD between 303 pruned atom pairs is 0.597 angstroms; (across all 445 pairs: |
8096 | | 4.918) |
8097 | | |
8098 | | |
8099 | | > hide #!25 models |
8100 | | |
8101 | | > show #!7 models |
8102 | | |
8103 | | > hide #!7 models |
8104 | | |
8105 | | > show #!25 models |
8106 | | |
8107 | | > show #!7 models |
8108 | | |
8109 | | > hide #!7 models |
8110 | | |
8111 | | > show #!7 models |
8112 | | |
8113 | | > hide #!7 models |
8114 | | |
8115 | | > show #!7 models |
8116 | | |
8117 | | > hide #!7 models |
8118 | | |
8119 | | > show #!8 models |
8120 | | |
8121 | | > hide #!8 models |
8122 | | |
8123 | | > show #!7 models |
8124 | | |
8125 | | > hide #!7 models |
8126 | | |
8127 | | > open |
8128 | | > /Users/becca/Desktop/Postdoc/Phenix/W20_MM_cyto_COPI/PredictAndBuildCryoEM_1/PredictAndBuild_1/local_dock_and_rebuild_6_1/run_mr/A_fixed_model_2_A.pdb |
8129 | | |
8130 | | Summary of feedback from opening |
8131 | | /Users/becca/Desktop/Postdoc/Phenix/W20_MM_cyto_COPI/PredictAndBuildCryoEM_1/PredictAndBuild_1/local_dock_and_rebuild_6_1/run_mr/A_fixed_model_2_A.pdb |
8132 | | --- |
8133 | | warning | Ignored bad PDB record found on line 9748 |
8134 | | |
8135 | | Chain information for A_fixed_model_2_A.pdb #51 |
8136 | | --- |
8137 | | Chain | Description |
8138 | | A | No description available |
8139 | | |
8140 | | Computing secondary structure |
8141 | | |
8142 | | > open |
8143 | | > /Users/becca/Desktop/Postdoc/Phenix/W20_MM_cyto_COPI/PredictAndBuildCryoEM_1/PredictAndBuild_1/local_dock_and_rebuild_6_1/run_mr/B_fixed_model_2_B.pdb |
8144 | | |
8145 | | Summary of feedback from opening |
8146 | | /Users/becca/Desktop/Postdoc/Phenix/W20_MM_cyto_COPI/PredictAndBuildCryoEM_1/PredictAndBuild_1/local_dock_and_rebuild_6_1/run_mr/B_fixed_model_2_B.pdb |
8147 | | --- |
8148 | | warning | Ignored bad PDB record found on line 7507 |
8149 | | |
8150 | | Chain information for B_fixed_model_2_B.pdb #52 |
8151 | | --- |
8152 | | Chain | Description |
8153 | | B | No description available |
8154 | | |
8155 | | Computing secondary structure |
8156 | | |
8157 | | > open |
8158 | | > /Users/becca/Desktop/Postdoc/Phenix/W20_MM_cyto_COPI/PredictAndBuildCryoEM_1/PredictAndBuild_1/local_dock_and_rebuild_6_1/run_mr/C1_fixed_model_2_C1.pdb |
8159 | | |
8160 | | Summary of feedback from opening |
8161 | | /Users/becca/Desktop/Postdoc/Phenix/W20_MM_cyto_COPI/PredictAndBuildCryoEM_1/PredictAndBuild_1/local_dock_and_rebuild_6_1/run_mr/C1_fixed_model_2_C1.pdb |
8162 | | --- |
8163 | | warning | Ignored bad PDB record found on line 3967 |
8164 | | |
8165 | | Chain information for C1_fixed_model_2_C1.pdb #53 |
8166 | | --- |
8167 | | Chain | Description |
8168 | | C1 | No description available |
8169 | | |
8170 | | Computing secondary structure |
8171 | | |
8172 | | > open |
8173 | | > /Users/becca/Desktop/Postdoc/Phenix/W20_MM_cyto_COPI/PredictAndBuildCryoEM_1/PredictAndBuild_1/local_dock_and_rebuild_6_1/run_mr/D1_fixed_model_2_D1.pdb |
8174 | | |
8175 | | Summary of feedback from opening |
8176 | | /Users/becca/Desktop/Postdoc/Phenix/W20_MM_cyto_COPI/PredictAndBuildCryoEM_1/PredictAndBuild_1/local_dock_and_rebuild_6_1/run_mr/D1_fixed_model_2_D1.pdb |
8177 | | --- |
8178 | | warning | Ignored bad PDB record found on line 3307 |
8179 | | |
8180 | | Chain information for D1_fixed_model_2_D1.pdb #54 |
8181 | | --- |
8182 | | Chain | Description |
8183 | | D1 | No description available |
8184 | | |
8185 | | Computing secondary structure |
8186 | | |
8187 | | > open |
8188 | | > /Users/becca/Desktop/Postdoc/Phenix/W20_MM_cyto_COPI/PredictAndBuildCryoEM_1/PredictAndBuild_1/local_dock_and_rebuild_6_1/run_mr/E2_full_shifted_sm_1.pdb |
8189 | | |
8190 | | Summary of feedback from opening |
8191 | | /Users/becca/Desktop/Postdoc/Phenix/W20_MM_cyto_COPI/PredictAndBuildCryoEM_1/PredictAndBuild_1/local_dock_and_rebuild_6_1/run_mr/E2_full_shifted_sm_1.pdb |
8192 | | --- |
8193 | | warning | Ignored bad PDB record found on line 4486 |
8194 | | |
8195 | | Chain information for E2_full_shifted_sm_1.pdb #55 |
8196 | | --- |
8197 | | Chain | Description |
8198 | | E2 | No description available |
8199 | | |
8200 | | Computing secondary structure |
8201 | | |
8202 | | > open |
8203 | | > /Users/becca/Desktop/Postdoc/Phenix/W20_MM_cyto_COPI/PredictAndBuildCryoEM_1/PredictAndBuild_1/local_dock_and_rebuild_6_1/run_mr/F_fixed_model_2_F.pdb |
8204 | | |
8205 | | Summary of feedback from opening |
8206 | | /Users/becca/Desktop/Postdoc/Phenix/W20_MM_cyto_COPI/PredictAndBuildCryoEM_1/PredictAndBuild_1/local_dock_and_rebuild_6_1/run_mr/F_fixed_model_2_F.pdb |
8207 | | --- |
8208 | | warning | Ignored bad PDB record found on line 1426 |
8209 | | |
8210 | | Chain information for F_fixed_model_2_F.pdb #56 |
8211 | | --- |
8212 | | Chain | Description |
8213 | | F | No description available |
8214 | | |
8215 | | Computing secondary structure |
8216 | | |
8217 | | > hide #!25 models |
8218 | | |
8219 | | > show #!1 models |
8220 | | |
8221 | | > hide #56 models |
8222 | | |
8223 | | > hide #!55 models |
8224 | | |
8225 | | > hide #!54 models |
8226 | | |
8227 | | > hide #!53 models |
8228 | | |
8229 | | > hide #52 models |
8230 | | |
8231 | | > hide #51 models |
8232 | | |
8233 | | > hide #!1 models |
8234 | | |
8235 | | > show #!2 models |
8236 | | |
8237 | | > color #2 darkgrey models |
8238 | | |
8239 | | > color #2 #a9a9a980 models |
8240 | | |
8241 | | > hide #!50 models |
8242 | | |
8243 | | > show #!47 models |
8244 | | |
8245 | | > fitmap #47 inMap #2 |
8246 | | |
8247 | | Fit molecules W20_C1_initial_model_v3.pdb D (#47.1), |
8248 | | W20_C1_initial_model_v3.pdb E (#47.2), W20_C1_initial_model_v3.pdb F (#47.3), |
8249 | | W20_C1_initial_model_v3.pdb G (#47.4), W20_C1_initial_model_v3.pdb H (#47.5), |
8250 | | W20_C1_initial_model_v3.pdb I (#47.6), W20_C1_initial_model_v3.pdb J (#47.7), |
8251 | | W20_C1_initial_model_v3.pdb K (#47.8), W20_C1_initial_model_v3.pdb L (#47.9) |
8252 | | to map W20_J3623_consensus_structure.mrc (#2) using 28705 atoms |
8253 | | average map value = 0.26, steps = 44 |
8254 | | shifted from previous position = 0.0505 |
8255 | | rotated from previous position = 0.167 degrees |
8256 | | atoms outside contour = 16402, contour level = 0.25746 |
8257 | | |
8258 | | Position of W20_C1_initial_model_v3.pdb D (#47.1) relative to |
8259 | | W20_J3623_consensus_structure.mrc (#2) coordinates: |
8260 | | Matrix rotation and translation |
8261 | | 0.99995987 -0.00698720 0.00560699 0.30640707 |
8262 | | 0.00699483 0.99997464 -0.00134153 -1.12673692 |
8263 | | -0.00559747 0.00138070 0.99998338 0.47678248 |
8264 | | Axis 0.15020785 0.61824110 0.77150214 |
8265 | | Axis point 136.27032262 49.14800743 0.00000000 |
8266 | | Rotation angle (degrees) 0.51919628 |
8267 | | Shift along axis -0.28273161 |
8268 | | |
8269 | | Position of W20_C1_initial_model_v3.pdb E (#47.2) relative to |
8270 | | W20_J3623_consensus_structure.mrc (#2) coordinates: |
8271 | | Matrix rotation and translation |
8272 | | 0.99995987 -0.00698720 0.00560699 0.30640707 |
8273 | | 0.00699483 0.99997464 -0.00134153 -1.12673692 |
8274 | | -0.00559747 0.00138070 0.99998338 0.47678248 |
8275 | | Axis 0.15020785 0.61824110 0.77150214 |
8276 | | Axis point 136.27032262 49.14800743 0.00000000 |
8277 | | Rotation angle (degrees) 0.51919628 |
8278 | | Shift along axis -0.28273161 |
8279 | | |
8280 | | Position of W20_C1_initial_model_v3.pdb F (#47.3) relative to |
8281 | | W20_J3623_consensus_structure.mrc (#2) coordinates: |
8282 | | Matrix rotation and translation |
8283 | | 0.99995987 -0.00698720 0.00560699 0.30640707 |
8284 | | 0.00699483 0.99997464 -0.00134153 -1.12673692 |
8285 | | -0.00559747 0.00138070 0.99998338 0.47678248 |
8286 | | Axis 0.15020785 0.61824110 0.77150214 |
8287 | | Axis point 136.27032262 49.14800743 0.00000000 |
8288 | | Rotation angle (degrees) 0.51919628 |
8289 | | Shift along axis -0.28273161 |
8290 | | |
8291 | | Position of W20_C1_initial_model_v3.pdb G (#47.4) relative to |
8292 | | W20_J3623_consensus_structure.mrc (#2) coordinates: |
8293 | | Matrix rotation and translation |
8294 | | 0.99995987 -0.00698720 0.00560699 0.30640707 |
8295 | | 0.00699483 0.99997464 -0.00134153 -1.12673692 |
8296 | | -0.00559747 0.00138070 0.99998338 0.47678248 |
8297 | | Axis 0.15020785 0.61824110 0.77150214 |
8298 | | Axis point 136.27032262 49.14800743 0.00000000 |
8299 | | Rotation angle (degrees) 0.51919628 |
8300 | | Shift along axis -0.28273161 |
8301 | | |
8302 | | Position of W20_C1_initial_model_v3.pdb H (#47.5) relative to |
8303 | | W20_J3623_consensus_structure.mrc (#2) coordinates: |
8304 | | Matrix rotation and translation |
8305 | | 0.99995987 -0.00698720 0.00560699 0.30640707 |
8306 | | 0.00699483 0.99997464 -0.00134153 -1.12673692 |
8307 | | -0.00559747 0.00138070 0.99998338 0.47678248 |
8308 | | Axis 0.15020785 0.61824110 0.77150214 |
8309 | | Axis point 136.27032262 49.14800743 0.00000000 |
8310 | | Rotation angle (degrees) 0.51919628 |
8311 | | Shift along axis -0.28273161 |
8312 | | |
8313 | | Position of W20_C1_initial_model_v3.pdb I (#47.6) relative to |
8314 | | W20_J3623_consensus_structure.mrc (#2) coordinates: |
8315 | | Matrix rotation and translation |
8316 | | 0.99995987 -0.00698720 0.00560699 0.30640707 |
8317 | | 0.00699483 0.99997464 -0.00134153 -1.12673692 |
8318 | | -0.00559747 0.00138070 0.99998338 0.47678248 |
8319 | | Axis 0.15020785 0.61824110 0.77150214 |
8320 | | Axis point 136.27032262 49.14800743 0.00000000 |
8321 | | Rotation angle (degrees) 0.51919628 |
8322 | | Shift along axis -0.28273161 |
8323 | | |
8324 | | Position of W20_C1_initial_model_v3.pdb J (#47.7) relative to |
8325 | | W20_J3623_consensus_structure.mrc (#2) coordinates: |
8326 | | Matrix rotation and translation |
8327 | | 0.99995987 -0.00698720 0.00560699 0.30640707 |
8328 | | 0.00699483 0.99997464 -0.00134153 -1.12673692 |
8329 | | -0.00559747 0.00138070 0.99998338 0.47678248 |
8330 | | Axis 0.15020785 0.61824110 0.77150214 |
8331 | | Axis point 136.27032262 49.14800743 0.00000000 |
8332 | | Rotation angle (degrees) 0.51919628 |
8333 | | Shift along axis -0.28273161 |
8334 | | |
8335 | | Position of W20_C1_initial_model_v3.pdb K (#47.8) relative to |
8336 | | W20_J3623_consensus_structure.mrc (#2) coordinates: |
8337 | | Matrix rotation and translation |
8338 | | 0.99995987 -0.00698720 0.00560699 0.30640707 |
8339 | | 0.00699483 0.99997464 -0.00134153 -1.12673692 |
8340 | | -0.00559747 0.00138070 0.99998338 0.47678248 |
8341 | | Axis 0.15020785 0.61824110 0.77150214 |
8342 | | Axis point 136.27032262 49.14800743 0.00000000 |
8343 | | Rotation angle (degrees) 0.51919628 |
8344 | | Shift along axis -0.28273161 |
8345 | | |
8346 | | Position of W20_C1_initial_model_v3.pdb L (#47.9) relative to |
8347 | | W20_J3623_consensus_structure.mrc (#2) coordinates: |
8348 | | Matrix rotation and translation |
8349 | | 0.99995987 -0.00698720 0.00560699 0.30640707 |
8350 | | 0.00699483 0.99997464 -0.00134153 -1.12673692 |
8351 | | -0.00559747 0.00138070 0.99998338 0.47678248 |
8352 | | Axis 0.15020785 0.61824110 0.77150214 |
8353 | | Axis point 136.27032262 49.14800743 0.00000000 |
8354 | | Rotation angle (degrees) 0.51919628 |
8355 | | Shift along axis -0.28273161 |
8356 | | |
8357 | | |
8358 | | > fitmap #47.1 inMap #2 |
8359 | | |
8360 | | Fit molecule W20_C1_initial_model_v3.pdb D (#47.1) to map |
8361 | | W20_J3623_consensus_structure.mrc (#2) using 4763 atoms |
8362 | | average map value = 0.3391, steps = 60 |
8363 | | shifted from previous position = 0.277 |
8364 | | rotated from previous position = 0.317 degrees |
8365 | | atoms outside contour = 1269, contour level = 0.25746 |
8366 | | |
8367 | | Position of W20_C1_initial_model_v3.pdb D (#47.1) relative to |
8368 | | W20_J3623_consensus_structure.mrc (#2) coordinates: |
8369 | | Matrix rotation and translation |
8370 | | 0.99993645 -0.00554192 0.00981798 -0.36993206 |
8371 | | 0.00552327 0.99998289 0.00192574 -1.44337504 |
8372 | | -0.00982849 -0.00187139 0.99994995 1.45151156 |
8373 | | Axis -0.16606190 0.85921136 0.48392075 |
8374 | | Axis point 170.89777041 0.00000000 46.84174593 |
8375 | | Rotation angle (degrees) 0.65506824 |
8376 | | Shift along axis -0.47631604 |
8377 | | |
8378 | | |
8379 | | > fitmap #47.2 inMap #2 |
8380 | | |
8381 | | Fit molecule W20_C1_initial_model_v3.pdb E (#47.2) to map |
8382 | | W20_J3623_consensus_structure.mrc (#2) using 1856 atoms |
8383 | | average map value = 0.298, steps = 52 |
8384 | | shifted from previous position = 0.74 |
8385 | | rotated from previous position = 0.516 degrees |
8386 | | atoms outside contour = 807, contour level = 0.25746 |
8387 | | |
8388 | | Position of W20_C1_initial_model_v3.pdb E (#47.2) relative to |
8389 | | W20_J3623_consensus_structure.mrc (#2) coordinates: |
8390 | | Matrix rotation and translation |
8391 | | 0.99985158 -0.01068491 0.01351472 -0.71801951 |
8392 | | 0.01073315 0.99993626 -0.00350238 -1.71551668 |
8393 | | -0.01347643 0.00364691 0.99990254 2.22722625 |
8394 | | Axis 0.20315994 0.76700206 0.60863280 |
8395 | | Axis point 169.67451772 0.00000000 53.39691400 |
8396 | | Rotation angle (degrees) 1.00818411 |
8397 | | Shift along axis -0.10611466 |
8398 | | |
8399 | | |
8400 | | > fitmap #47.3 inMap #2 |
8401 | | |
8402 | | Fit molecule W20_C1_initial_model_v3.pdb F (#47.3) to map |
8403 | | W20_J3623_consensus_structure.mrc (#2) using 7214 atoms |
8404 | | average map value = 0.1919, steps = 56 |
8405 | | shifted from previous position = 0.863 |
8406 | | rotated from previous position = 1.3 degrees |
8407 | | atoms outside contour = 5840, contour level = 0.25746 |
8408 | | |
8409 | | Position of W20_C1_initial_model_v3.pdb F (#47.3) relative to |
8410 | | W20_J3623_consensus_structure.mrc (#2) coordinates: |
8411 | | Matrix rotation and translation |
8412 | | 0.99983282 -0.01767194 -0.00469422 4.64975550 |
8413 | | 0.01774465 0.99971568 0.01592750 -6.16380107 |
8414 | | 0.00441141 -0.01600813 0.99986213 2.74940793 |
8415 | | Axis -0.65778440 -0.18755047 0.72948236 |
8416 | | Axis point 350.49529384 263.63816940 0.00000000 |
8417 | | Rotation angle (degrees) 1.39100037 |
8418 | | Shift along axis 0.10313173 |
8419 | | |
8420 | | |
8421 | | > fitmap #47.4 inMap #2 |
8422 | | |
8423 | | Fit molecule W20_C1_initial_model_v3.pdb G (#47.4) to map |
8424 | | W20_J3623_consensus_structure.mrc (#2) using 1872 atoms |
8425 | | average map value = 0.2684, steps = 48 |
8426 | | shifted from previous position = 0.694 |
8427 | | rotated from previous position = 1.52 degrees |
8428 | | atoms outside contour = 902, contour level = 0.25746 |
8429 | | |
8430 | | Position of W20_C1_initial_model_v3.pdb G (#47.4) relative to |
8431 | | W20_J3623_consensus_structure.mrc (#2) coordinates: |
8432 | | Matrix rotation and translation |
8433 | | 0.99999905 0.00004391 -0.00137935 0.08316312 |
8434 | | -0.00001180 0.99972908 0.02327568 -3.69665287 |
8435 | | 0.00138000 -0.02327564 0.99972813 3.29847865 |
8436 | | Axis -0.99824711 -0.05917154 -0.00119461 |
8437 | | Axis point 0.00000000 139.85035535 160.11317726 |
8438 | | Rotation angle (degrees) 1.33605990 |
8439 | | Shift along axis 0.13177890 |
8440 | | |
8441 | | |
8442 | | > fitmap #47.5 inMap #2 |
8443 | | |
8444 | | Fit molecule W20_C1_initial_model_v3.pdb H (#47.5) to map |
8445 | | W20_J3623_consensus_structure.mrc (#2) using 4752 atoms |
8446 | | average map value = 0.2052, steps = 56 |
8447 | | shifted from previous position = 0.558 |
8448 | | rotated from previous position = 2.67 degrees |
8449 | | atoms outside contour = 3635, contour level = 0.25746 |
8450 | | |
8451 | | Position of W20_C1_initial_model_v3.pdb H (#47.5) relative to |
8452 | | W20_J3623_consensus_structure.mrc (#2) coordinates: |
8453 | | Matrix rotation and translation |
8454 | | 0.99873328 -0.02416117 0.04413700 -1.58450683 |
8455 | | 0.02335616 0.99955296 0.01866451 -4.67495439 |
8456 | | -0.04456822 -0.01760999 0.99885112 6.98195313 |
8457 | | Axis -0.33911247 0.82926157 0.44421615 |
8458 | | Axis point 157.93980007 0.00000000 42.26069084 |
8459 | | Rotation angle (degrees) 3.06589704 |
8460 | | Shift along axis -0.23793767 |
8461 | | |
8462 | | |
8463 | | > fitmap #47.6 inMap #2 |
8464 | | |
8465 | | Fit molecule W20_C1_initial_model_v3.pdb I (#47.6) to map |
8466 | | W20_J3623_consensus_structure.mrc (#2) using 2488 atoms |
8467 | | average map value = 0.3942, steps = 48 |
8468 | | shifted from previous position = 0.0985 |
8469 | | rotated from previous position = 0.351 degrees |
8470 | | atoms outside contour = 688, contour level = 0.25746 |
8471 | | |
8472 | | Position of W20_C1_initial_model_v3.pdb I (#47.6) relative to |
8473 | | W20_J3623_consensus_structure.mrc (#2) coordinates: |
8474 | | Matrix rotation and translation |
8475 | | 0.99997623 -0.00687548 -0.00051167 1.19535071 |
8476 | | 0.00687477 0.99997543 -0.00137176 -1.17275950 |
8477 | | 0.00052109 0.00136821 0.99999893 -0.25085656 |
8478 | | Axis 0.19489694 -0.07346086 0.97806886 |
8479 | | Axis point 170.41255683 171.17704205 0.00000000 |
8480 | | Rotation angle (degrees) 0.40275148 |
8481 | | Shift along axis 0.07376713 |
8482 | | |
8483 | | |
8484 | | > fitmap #47.7 inMap #2 |
8485 | | |
8486 | | Fit molecule W20_C1_initial_model_v3.pdb J (#47.7) to map |
8487 | | W20_J3623_consensus_structure.mrc (#2) using 2879 atoms |
8488 | | average map value = 0.2974, steps = 40 |
8489 | | shifted from previous position = 0.0947 |
8490 | | rotated from previous position = 0.56 degrees |
8491 | | atoms outside contour = 1205, contour level = 0.25746 |
8492 | | |
8493 | | Position of W20_C1_initial_model_v3.pdb J (#47.7) relative to |
8494 | | W20_J3623_consensus_structure.mrc (#2) coordinates: |
8495 | | Matrix rotation and translation |
8496 | | 0.99998391 0.00266199 0.00500985 -1.32620675 |
8497 | | -0.00264770 0.99999241 -0.00285677 0.77420871 |
8498 | | -0.00501741 0.00284346 0.99998337 0.17173811 |
8499 | | Axis 0.44891807 0.78969202 -0.41816155 |
8500 | | Axis point 28.71795251 0.00000000 259.81340923 |
8501 | | Rotation angle (degrees) 0.36376436 |
8502 | | Shift along axis -0.05578601 |
8503 | | |
8504 | | |
8505 | | > fitmap #47.8 inMap #2 |
8506 | | |
8507 | | Fit molecule W20_C1_initial_model_v3.pdb K (#47.8) to map |
8508 | | W20_J3623_consensus_structure.mrc (#2) using 1681 atoms |
8509 | | average map value = 0.2582, steps = 2000 |
8510 | | shifted from previous position = 3.05 |
8511 | | rotated from previous position = 19.1 degrees |
8512 | | atoms outside contour = 877, contour level = 0.25746 |
8513 | | |
8514 | | Position of W20_C1_initial_model_v3.pdb K (#47.8) relative to |
8515 | | W20_J3623_consensus_structure.mrc (#2) coordinates: |
8516 | | Matrix rotation and translation |
8517 | | 0.94200137 -0.31946272 0.10284450 49.35608426 |
8518 | | 0.31956519 0.94743045 0.01592570 -41.58693728 |
8519 | | -0.10252568 0.01786349 0.99456995 13.43084868 |
8520 | | Axis 0.00288696 0.30596518 0.95203833 |
8521 | | Axis point 151.23068995 127.03929522 0.00000000 |
8522 | | Rotation angle (degrees) 19.60965973 |
8523 | | Shift along axis 0.20501701 |
8524 | | |
8525 | | |
8526 | | > fitmap #47.9 inMap #2 |
8527 | | |
8528 | | Fit molecule W20_C1_initial_model_v3.pdb L (#47.9) to map |
8529 | | W20_J3623_consensus_structure.mrc (#2) using 1200 atoms |
8530 | | average map value = 0.225, steps = 104 |
8531 | | shifted from previous position = 7.72 |
8532 | | rotated from previous position = 16.8 degrees |
8533 | | atoms outside contour = 923, contour level = 0.25746 |
8534 | | |
8535 | | Position of W20_C1_initial_model_v3.pdb L (#47.9) relative to |
8536 | | W20_J3623_consensus_structure.mrc (#2) coordinates: |
8537 | | Matrix rotation and translation |
8538 | | 0.95622273 0.06913903 0.28435522 -32.79890580 |
8539 | | -0.07184973 0.99741506 -0.00090016 15.80706570 |
8540 | | -0.28368241 -0.01957009 0.95871857 38.27014130 |
8541 | | Axis -0.03188330 0.97005801 -0.24077150 |
8542 | | Axis point 121.59404774 0.00000000 133.26118376 |
8543 | | Rotation angle (degrees) 17.02478724 |
8544 | | Shift along axis 7.16514893 |
8545 | | |
8546 | | |
8547 | | > hide #!47 models |
8548 | | |
8549 | | > show #!48 models |
8550 | | |
8551 | | > split #48 chains |
8552 | | |
8553 | | Split W20_C1_initial_model_v3_no_wat_lig.pdb (#48) into 9 models |
8554 | | Chain information for W20_C1_initial_model_v3_no_wat_lig.pdb D #48.1 |
8555 | | --- |
8556 | | Chain | Description |
8557 | | D | No description available |
8558 | | |
8559 | | Chain information for W20_C1_initial_model_v3_no_wat_lig.pdb E #48.2 |
8560 | | --- |
8561 | | Chain | Description |
8562 | | E | No description available |
8563 | | |
8564 | | Chain information for W20_C1_initial_model_v3_no_wat_lig.pdb F #48.3 |
8565 | | --- |
8566 | | Chain | Description |
8567 | | F | No description available |
8568 | | |
8569 | | Chain information for W20_C1_initial_model_v3_no_wat_lig.pdb G #48.4 |
8570 | | --- |
8571 | | Chain | Description |
8572 | | G | No description available |
8573 | | |
8574 | | Chain information for W20_C1_initial_model_v3_no_wat_lig.pdb H #48.5 |
8575 | | --- |
8576 | | Chain | Description |
8577 | | H | No description available |
8578 | | |
8579 | | Chain information for W20_C1_initial_model_v3_no_wat_lig.pdb I #48.6 |
8580 | | --- |
8581 | | Chain | Description |
8582 | | I | No description available |
8583 | | |
8584 | | Chain information for W20_C1_initial_model_v3_no_wat_lig.pdb J #48.7 |
8585 | | --- |
8586 | | Chain | Description |
8587 | | J | No description available |
8588 | | |
8589 | | Chain information for W20_C1_initial_model_v3_no_wat_lig.pdb K #48.8 |
8590 | | --- |
8591 | | Chain | Description |
8592 | | K | No description available |
8593 | | |
8594 | | Chain information for W20_C1_initial_model_v3_no_wat_lig.pdb L #48.9 |
8595 | | --- |
8596 | | Chain | Description |
8597 | | L | No description available |
8598 | | |
8599 | | |
8600 | | > fitmap #48.9 inMap #2 |
8601 | | |
8602 | | Fit molecule W20_C1_initial_model_v3_no_wat_lig.pdb L (#48.9) to map |
8603 | | W20_J3623_consensus_structure.mrc (#2) using 1200 atoms |
8604 | | average map value = 0.225, steps = 104 |
8605 | | shifted from previous position = 7.35 |
8606 | | rotated from previous position = 17 degrees |
8607 | | atoms outside contour = 923, contour level = 0.25746 |
8608 | | |
8609 | | Position of W20_C1_initial_model_v3_no_wat_lig.pdb L (#48.9) relative to |
8610 | | W20_J3623_consensus_structure.mrc (#2) coordinates: |
8611 | | Matrix rotation and translation |
8612 | | 0.95618362 0.06935484 0.28443415 -32.83561380 |
8613 | | -0.07187151 0.99741263 -0.00159273 15.88500353 |
8614 | | -0.28380868 -0.01891977 0.95869426 38.21611444 |
8615 | | Axis -0.02957914 0.97005222 -0.24108871 |
8616 | | Axis point 121.32947981 0.00000000 133.38706461 |
8617 | | Rotation angle (degrees) 17.03122986 |
8618 | | Shift along axis 7.16705836 |
8619 | | |
8620 | | |
8621 | | > fitmap #48.8 inMap #2 |
8622 | | |
8623 | | Fit molecule W20_C1_initial_model_v3_no_wat_lig.pdb K (#48.8) to map |
8624 | | W20_J3623_consensus_structure.mrc (#2) using 1681 atoms |
8625 | | average map value = 0.2582, steps = 2000 |
8626 | | shifted from previous position = 3 |
8627 | | rotated from previous position = 19.6 degrees |
8628 | | atoms outside contour = 877, contour level = 0.25746 |
8629 | | |
8630 | | Position of W20_C1_initial_model_v3_no_wat_lig.pdb K (#48.8) relative to |
8631 | | W20_J3623_consensus_structure.mrc (#2) coordinates: |
8632 | | Matrix rotation and translation |
8633 | | 0.94200226 -0.31945879 0.10284856 49.35474105 |
8634 | | 0.31956109 0.94743180 0.01592780 -41.58682474 |
8635 | | -0.10253028 0.01786237 0.99456949 13.43178853 |
8636 | | Axis 0.00288219 0.30598035 0.95203347 |
8637 | | Axis point 151.23135956 127.03695546 0.00000000 |
8638 | | Rotation angle (degrees) 19.60950766 |
8639 | | Shift along axis 0.20501096 |
8640 | | |
8641 | | |
8642 | | > fitmap #48.7 inMap #2 |
8643 | | |
8644 | | Fit molecule W20_C1_initial_model_v3_no_wat_lig.pdb J (#48.7) to map |
8645 | | W20_J3623_consensus_structure.mrc (#2) using 2879 atoms |
8646 | | average map value = 0.2974, steps = 60 |
8647 | | shifted from previous position = 0.232 |
8648 | | rotated from previous position = 0.38 degrees |
8649 | | atoms outside contour = 1205, contour level = 0.25746 |
8650 | | |
8651 | | Position of W20_C1_initial_model_v3_no_wat_lig.pdb J (#48.7) relative to |
8652 | | W20_J3623_consensus_structure.mrc (#2) coordinates: |
8653 | | Matrix rotation and translation |
8654 | | 0.99998287 0.00312090 0.00495157 -1.40345048 |
8655 | | -0.00310539 0.99999025 -0.00313821 0.88665991 |
8656 | | -0.00496131 0.00312278 0.99998282 0.10130440 |
8657 | | Axis 0.47162964 0.74671971 -0.46901508 |
8658 | | Axis point 14.97777640 0.00000000 278.97871593 |
8659 | | Rotation angle (degrees) 0.38031035 |
8660 | | Shift along axis -0.04733570 |
8661 | | |
8662 | | |
8663 | | > fitmap #48.6 inMap #2 |
8664 | | |
8665 | | Fit molecule W20_C1_initial_model_v3_no_wat_lig.pdb I (#48.6) to map |
8666 | | W20_J3623_consensus_structure.mrc (#2) using 2488 atoms |
8667 | | average map value = 0.3942, steps = 68 |
8668 | | shifted from previous position = 0.638 |
8669 | | rotated from previous position = 0.407 degrees |
8670 | | atoms outside contour = 688, contour level = 0.25746 |
8671 | | |
8672 | | Position of W20_C1_initial_model_v3_no_wat_lig.pdb I (#48.6) relative to |
8673 | | W20_J3623_consensus_structure.mrc (#2) coordinates: |
8674 | | Matrix rotation and translation |
8675 | | 0.99997568 -0.00695350 -0.00053467 1.20906358 |
8676 | | 0.00695275 0.99997488 -0.00137680 -1.17749870 |
8677 | | 0.00054423 0.00137305 0.99999891 -0.25259458 |
8678 | | Axis 0.19342635 -0.07589115 0.97817523 |
8679 | | Axis point 169.14439112 171.16881708 0.00000000 |
8680 | | Rotation angle (degrees) 0.40727684 |
8681 | | Shift along axis 0.07614472 |
8682 | | |
8683 | | |
8684 | | > fitmap #48.5 inMap #2 |
8685 | | |
8686 | | Fit molecule W20_C1_initial_model_v3_no_wat_lig.pdb H (#48.5) to map |
8687 | | W20_J3623_consensus_structure.mrc (#2) using 4752 atoms |
8688 | | average map value = 0.2052, steps = 56 |
8689 | | shifted from previous position = 0.398 |
8690 | | rotated from previous position = 3.04 degrees |
8691 | | atoms outside contour = 3633, contour level = 0.25746 |
8692 | | |
8693 | | Position of W20_C1_initial_model_v3_no_wat_lig.pdb H (#48.5) relative to |
8694 | | W20_J3623_consensus_structure.mrc (#2) coordinates: |
8695 | | Matrix rotation and translation |
8696 | | 0.99875256 -0.02376142 0.04391712 -1.59501966 |
8697 | | 0.02297566 0.99956832 0.01831081 -4.59906082 |
8698 | | -0.04433325 -0.01727895 0.99886736 6.92788561 |
8699 | | Axis -0.33570594 0.83243532 0.44085481 |
8700 | | Axis point 157.55390321 0.00000000 42.61926354 |
8701 | | Rotation angle (degrees) 3.03852113 |
8702 | | Shift along axis -0.23877143 |
8703 | | |
8704 | | |
8705 | | > fitmap #48.4 inMap #2 |
8706 | | |
8707 | | Fit molecule W20_C1_initial_model_v3_no_wat_lig.pdb G (#48.4) to map |
8708 | | W20_J3623_consensus_structure.mrc (#2) using 1872 atoms |
8709 | | average map value = 0.2684, steps = 52 |
8710 | | shifted from previous position = 0.66 |
8711 | | rotated from previous position = 1.34 degrees |
8712 | | atoms outside contour = 904, contour level = 0.25746 |
8713 | | |
8714 | | Position of W20_C1_initial_model_v3_no_wat_lig.pdb G (#48.4) relative to |
8715 | | W20_J3623_consensus_structure.mrc (#2) coordinates: |
8716 | | Matrix rotation and translation |
8717 | | 0.99999668 -0.00055774 -0.00251381 0.32378285 |
8718 | | 0.00061619 0.99972807 0.02331118 -3.79263384 |
8719 | | 0.00250012 -0.02331266 0.99972510 3.13991886 |
8720 | | Axis -0.99395584 -0.10688996 0.02502646 |
8721 | | Axis point 0.00000000 132.58526090 163.49903175 |
8722 | | Rotation angle (degrees) 1.34391993 |
8723 | | Shift along axis 0.16214970 |
8724 | | |
8725 | | |
8726 | | > fitmap #48.3 inMap #2 |
8727 | | |
8728 | | Fit molecule W20_C1_initial_model_v3_no_wat_lig.pdb F (#48.3) to map |
8729 | | W20_J3623_consensus_structure.mrc (#2) using 7214 atoms |
8730 | | average map value = 0.1919, steps = 52 |
8731 | | shifted from previous position = 1.21 |
8732 | | rotated from previous position = 1.38 degrees |
8733 | | atoms outside contour = 5841, contour level = 0.25746 |
8734 | | |
8735 | | Position of W20_C1_initial_model_v3_no_wat_lig.pdb F (#48.3) relative to |
8736 | | W20_J3623_consensus_structure.mrc (#2) coordinates: |
8737 | | Matrix rotation and translation |
8738 | | 0.99983183 -0.01777810 -0.00449952 4.61473624 |
8739 | | 0.01784647 0.99971839 0.01564141 -6.12537351 |
8740 | | 0.00422018 -0.01571908 0.99986754 2.73144245 |
8741 | | Axis -0.64988004 -0.18069744 0.73824411 |
8742 | | Axis point 346.08368056 260.84380532 -0.00000000 |
8743 | | Rotation angle (degrees) 1.38256144 |
8744 | | Shift along axis 0.12428563 |
8745 | | |
8746 | | |
8747 | | > fitmap #48.2 inMap #2 |
8748 | | |
8749 | | Fit molecule W20_C1_initial_model_v3_no_wat_lig.pdb E (#48.2) to map |
8750 | | W20_J3623_consensus_structure.mrc (#2) using 1856 atoms |
8751 | | average map value = 0.298, steps = 48 |
8752 | | shifted from previous position = 0.513 |
8753 | | rotated from previous position = 0.756 degrees |
8754 | | atoms outside contour = 811, contour level = 0.25746 |
8755 | | |
8756 | | Position of W20_C1_initial_model_v3_no_wat_lig.pdb E (#48.2) relative to |
8757 | | W20_J3623_consensus_structure.mrc (#2) coordinates: |
8758 | | Matrix rotation and translation |
8759 | | 0.99991922 -0.00558717 0.01141652 -1.06360279 |
8760 | | 0.00562764 0.99997799 -0.00351551 -0.81089288 |
8761 | | -0.01139663 0.00357947 0.99992865 1.85466537 |
8762 | | Axis 0.26882817 0.86438839 0.42492838 |
8763 | | Axis point 169.58714833 0.00000000 89.68331202 |
8764 | | Rotation angle (degrees) 0.75610423 |
8765 | | Shift along axis -0.19875283 |
8766 | | |
8767 | | |
8768 | | > fitmap #48.1 inMap #2 |
8769 | | |
8770 | | Fit molecule W20_C1_initial_model_v3_no_wat_lig.pdb D (#48.1) to map |
8771 | | W20_J3623_consensus_structure.mrc (#2) using 4763 atoms |
8772 | | average map value = 0.3391, steps = 52 |
8773 | | shifted from previous position = 0.556 |
8774 | | rotated from previous position = 0.656 degrees |
8775 | | atoms outside contour = 1271, contour level = 0.25746 |
8776 | | |
8777 | | Position of W20_C1_initial_model_v3_no_wat_lig.pdb D (#48.1) relative to |
8778 | | W20_J3623_consensus_structure.mrc (#2) coordinates: |
8779 | | Matrix rotation and translation |
8780 | | 0.99993657 -0.00557361 0.00978726 -0.36204539 |
8781 | | 0.00555366 0.99998245 0.00206434 -1.46156620 |
8782 | | -0.00979859 -0.00200985 0.99994997 1.46464333 |
8783 | | Axis -0.17797856 0.85559501 0.48608724 |
8784 | | Axis point 172.75674812 0.00000000 46.73299778 |
8785 | | Rotation angle (degrees) 0.65580741 |
8786 | | Shift along axis -0.47412801 |
8787 | | |
8788 | | |
8789 | | > hide #!48 models |
8790 | | |
8791 | | > show #!49 models |
8792 | | |
8793 | | > hide #!49 models |
8794 | | |
8795 | | > show #!50 models |
8796 | | |
8797 | | > fitmap #50 inMap #2 |
8798 | | |
8799 | | Fit molecule W20_C1_initial_model_no_wat_lig.pdb (#50) to map |
8800 | | W20_J3623_consensus_structure.mrc (#2) using 25890 atoms |
8801 | | average map value = 0.2892, steps = 92 |
8802 | | shifted from previous position = 6.37 |
8803 | | rotated from previous position = 6 degrees |
8804 | | atoms outside contour = 13101, contour level = 0.25746 |
8805 | | |
8806 | | Position of W20_C1_initial_model_no_wat_lig.pdb (#50) relative to |
8807 | | W20_J3623_consensus_structure.mrc (#2) coordinates: |
8808 | | Matrix rotation and translation |
8809 | | -0.92962183 0.23756050 -0.28172373 363.07149255 |
8810 | | -0.02176229 -0.79853926 -0.60154921 515.96615164 |
8811 | | -0.36787180 -0.55308232 0.74750939 166.87632524 |
8812 | | Axis 0.17464117 0.31041809 -0.93442018 |
8813 | | Axis point 215.59521690 272.59770300 0.00000000 |
8814 | | Rotation angle (degrees) 172.02382323 |
8815 | | Shift along axis 67.63985482 |
8816 | | |
8817 | | |
8818 | | > show #51 models |
8819 | | |
8820 | | > hide #51 models |
8821 | | |
8822 | | > hide #!50 models |
8823 | | |
8824 | | > show #!50 models |
8825 | | |
8826 | | > split #50 chains |
8827 | | |
8828 | | Split W20_C1_initial_model_no_wat_lig.pdb (#50) into 11 models |
8829 | | Chain information for W20_C1_initial_model_no_wat_lig.pdb A0 #50.1 |
8830 | | --- |
8831 | | Chain | Description |
8832 | | A0 | No description available |
8833 | | |
8834 | | Chain information for W20_C1_initial_model_no_wat_lig.pdb A1 #50.2 |
8835 | | --- |
8836 | | Chain | Description |
8837 | | A1 | No description available |
8838 | | |
8839 | | Chain information for W20_C1_initial_model_no_wat_lig.pdb A2 #50.3 |
8840 | | --- |
8841 | | Chain | Description |
8842 | | A2 | No description available |
8843 | | |
8844 | | Chain information for W20_C1_initial_model_no_wat_lig.pdb A3 #50.4 |
8845 | | --- |
8846 | | Chain | Description |
8847 | | A3 | No description available |
8848 | | |
8849 | | Chain information for W20_C1_initial_model_no_wat_lig.pdb A4 #50.5 |
8850 | | --- |
8851 | | Chain | Description |
8852 | | A4 | No description available |
8853 | | |
8854 | | Chain information for W20_C1_initial_model_no_wat_lig.pdb A5 #50.6 |
8855 | | --- |
8856 | | Chain | Description |
8857 | | A5 | No description available |
8858 | | |
8859 | | Chain information for W20_C1_initial_model_no_wat_lig.pdb A6 #50.7 |
8860 | | --- |
8861 | | Chain | Description |
8862 | | A6 | No description available |
8863 | | |
8864 | | Chain information for W20_C1_initial_model_no_wat_lig.pdb A7 #50.8 |
8865 | | --- |
8866 | | Chain | Description |
8867 | | A7 | No description available |
8868 | | |
8869 | | Chain information for W20_C1_initial_model_no_wat_lig.pdb A8 #50.9 |
8870 | | --- |
8871 | | Chain | Description |
8872 | | A8 | No description available |
8873 | | |
8874 | | Chain information for W20_C1_initial_model_no_wat_lig.pdb A9 #50.10 |
8875 | | --- |
8876 | | Chain | Description |
8877 | | A9 | No description available |
8878 | | |
8879 | | Chain information for W20_C1_initial_model_no_wat_lig.pdb BA #50.11 |
8880 | | --- |
8881 | | Chain | Description |
8882 | | BA | No description available |
8883 | | |
8884 | | |
8885 | | > hide #50.1 models |
8886 | | |
8887 | | > show #50.1 models |
8888 | | |
8889 | | > hide #50.1 models |
8890 | | |
8891 | | > show #50.1 models |
8892 | | |
8893 | | > hide #!50.2 models |
8894 | | |
8895 | | > show #!50.2 models |
8896 | | |
8897 | | > hide #!50.2 models |
8898 | | |
8899 | | > show #!50.2 models |
8900 | | |
8901 | | > hide #!50.3 models |
8902 | | |
8903 | | > show #!50.3 models |
8904 | | |
8905 | | > hide #!50.4 models |
8906 | | |
8907 | | > show #!50.4 models |
8908 | | |
8909 | | > hide #!50.3 models |
8910 | | |
8911 | | > show #!50.3 models |
8912 | | |
8913 | | > ui tool show Matchmaker |
8914 | | |
8915 | | > matchmaker #51 to #50.3 |
8916 | | |
8917 | | Computing secondary structure |
8918 | | Parameters |
8919 | | --- |
8920 | | Chain pairing | bb |
8921 | | Alignment algorithm | Needleman-Wunsch |
8922 | | Similarity matrix | BLOSUM-62 |
8923 | | SS fraction | 0.3 |
8924 | | Gap open (HH/SS/other) | 18/18/6 |
8925 | | Gap extend | 1 |
8926 | | SS matrix | | | H | S | O |
8927 | | ---|---|---|--- |
8928 | | H | 6 | -9 | -6 |
8929 | | S | | 6 | -6 |
8930 | | O | | | 4 |
8931 | | Iteration cutoff | 2 |
8932 | | |
8933 | | Matchmaker W20_C1_initial_model_no_wat_lig.pdb A2, chain A2 (#50.3) with |
8934 | | A_fixed_model_2_A.pdb, chain A (#51), sequence alignment score = 2192.7 |
8935 | | RMSD between 285 pruned atom pairs is 0.343 angstroms; (across all 445 pairs: |
8936 | | 7.570) |
8937 | | |
8938 | | |
8939 | | > show #51 models |
8940 | | |
8941 | | > hide #!50.7 models |
8942 | | |
8943 | | > show #!50.7 models |
8944 | | |
8945 | | > ui tool show Matchmaker |
8946 | | |
8947 | | > show #52 models |
8948 | | |
8949 | | > hide #52 models |
8950 | | |
8951 | | > show #!53 models |
8952 | | |
8953 | | > matchmaker #!53 to #50.7 |
8954 | | |
8955 | | Computing secondary structure |
8956 | | Parameters |
8957 | | --- |
8958 | | Chain pairing | bb |
8959 | | Alignment algorithm | Needleman-Wunsch |
8960 | | Similarity matrix | BLOSUM-62 |
8961 | | SS fraction | 0.3 |
8962 | | Gap open (HH/SS/other) | 18/18/6 |
8963 | | Gap extend | 1 |
8964 | | SS matrix | | | H | S | O |
8965 | | ---|---|---|--- |
8966 | | H | 6 | -9 | -6 |
8967 | | S | | 6 | -6 |
8968 | | O | | | 4 |
8969 | | Iteration cutoff | 2 |
8970 | | |
8971 | | Matchmaker W20_C1_initial_model_no_wat_lig.pdb A6, chain A6 (#50.7) with |
8972 | | C1_fixed_model_2_C1.pdb, chain C1 (#53), sequence alignment score = 1136.3 |
8973 | | RMSD between 188 pruned atom pairs is 0.567 angstroms; (across all 446 pairs: |
8974 | | 42.070) |
8975 | | |
8976 | | |
8977 | | > hide #!53 models |
8978 | | |
8979 | | > show #!54 models |
8980 | | |
8981 | | > hide #!50.8 models |
8982 | | |
8983 | | > show #!50.8 models |
8984 | | |
8985 | | > hide #!50.8 models |
8986 | | |
8987 | | > show #!50.8 models |
8988 | | |
8989 | | > ui tool show Matchmaker |
8990 | | |
8991 | | > hide #50.6 models |
8992 | | |
8993 | | > show #50.6 models |
8994 | | |
8995 | | > hide #!50.4 models |
8996 | | |
8997 | | > show #!50.4 models |
8998 | | |
8999 | | > matchmaker #52 to #50.4 |
9000 | | |
9001 | | Computing secondary structure |
9002 | | Parameters |
9003 | | --- |
9004 | | Chain pairing | bb |
9005 | | Alignment algorithm | Needleman-Wunsch |
9006 | | Similarity matrix | BLOSUM-62 |
9007 | | SS fraction | 0.3 |
9008 | | Gap open (HH/SS/other) | 18/18/6 |
9009 | | Gap extend | 1 |
9010 | | SS matrix | | | H | S | O |
9011 | | ---|---|---|--- |
9012 | | H | 6 | -9 | -6 |
9013 | | S | | 6 | -6 |
9014 | | O | | | 4 |
9015 | | Iteration cutoff | 2 |
9016 | | |
9017 | | Matchmaker W20_C1_initial_model_no_wat_lig.pdb A3, chain A3 (#50.4) with |
9018 | | B_fixed_model_2_B.pdb, chain B (#52), sequence alignment score = 2854.3 |
9019 | | RMSD between 172 pruned atom pairs is 0.254 angstroms; (across all 567 pairs: |
9020 | | 4.295) |
9021 | | |
9022 | | |
9023 | | > show #52 models |
9024 | | |
9025 | | > hide #52 models |
9026 | | |
9027 | | > ui tool show Matchmaker |
9028 | | |
9029 | | > matchmaker #!54 to #50.8 |
9030 | | |
9031 | | Computing secondary structure |
9032 | | Parameters |
9033 | | --- |
9034 | | Chain pairing | bb |
9035 | | Alignment algorithm | Needleman-Wunsch |
9036 | | Similarity matrix | BLOSUM-62 |
9037 | | SS fraction | 0.3 |
9038 | | Gap open (HH/SS/other) | 18/18/6 |
9039 | | Gap extend | 1 |
9040 | | SS matrix | | | H | S | O |
9041 | | ---|---|---|--- |
9042 | | H | 6 | -9 | -6 |
9043 | | S | | 6 | -6 |
9044 | | O | | | 4 |
9045 | | Iteration cutoff | 2 |
9046 | | |
9047 | | Matchmaker W20_C1_initial_model_no_wat_lig.pdb A7, chain A7 (#50.8) with |
9048 | | D1_fixed_model_2_D1.pdb, chain D1 (#54), sequence alignment score = 660 |
9049 | | RMSD between 129 pruned atom pairs is 0.312 angstroms; (across all 131 pairs: |
9050 | | 0.453) |
9051 | | |
9052 | | |
9053 | | > hide #!54 models |
9054 | | |
9055 | | > show #!55 models |
9056 | | |
9057 | | > hide #!55 models |
9058 | | |
9059 | | > show #!55 models |
9060 | | |
9061 | | > hide #!50.10 models |
9062 | | |
9063 | | > show #!50.10 models |
9064 | | |
9065 | | > hide #!50.10 models |
9066 | | |
9067 | | > show #!50.10 models |
9068 | | |
9069 | | > ui tool show Matchmaker |
9070 | | |
9071 | | > matchmaker #!55 to #50.10 |
9072 | | |
9073 | | Computing secondary structure |
9074 | | Parameters |
9075 | | --- |
9076 | | Chain pairing | bb |
9077 | | Alignment algorithm | Needleman-Wunsch |
9078 | | Similarity matrix | BLOSUM-62 |
9079 | | SS fraction | 0.3 |
9080 | | Gap open (HH/SS/other) | 18/18/6 |
9081 | | Gap extend | 1 |
9082 | | SS matrix | | | H | S | O |
9083 | | ---|---|---|--- |
9084 | | H | 6 | -9 | -6 |
9085 | | S | | 6 | -6 |
9086 | | O | | | 4 |
9087 | | Iteration cutoff | 2 |
9088 | | |
9089 | | Matchmaker W20_C1_initial_model_no_wat_lig.pdb A9, chain A9 (#50.10) with |
9090 | | E2_full_shifted_sm_1.pdb, chain E2 (#55), sequence alignment score = 1394.2 |
9091 | | RMSD between 277 pruned atom pairs is 0.670 angstroms; (across all 277 pairs: |
9092 | | 0.670) |
9093 | | |
9094 | | |
9095 | | > hide #!55 models |
9096 | | |
9097 | | > show #!55 models |
9098 | | |
9099 | | > hide #!55 models |
9100 | | |
9101 | | > show #!55 models |
9102 | | |
9103 | | > hide #!50.10 models |
9104 | | |
9105 | | > show #!50.10 models |
9106 | | |
9107 | | > hide #!55 models |
9108 | | |
9109 | | > show #56 models |
9110 | | |
9111 | | > hide #50.11 models |
9112 | | |
9113 | | > show #50.11 models |
9114 | | |
9115 | | > ui tool show Matchmaker |
9116 | | |
9117 | | > matchmaker #56 to #50.11 |
9118 | | |
9119 | | Computing secondary structure |
9120 | | Parameters |
9121 | | --- |
9122 | | Chain pairing | bb |
9123 | | Alignment algorithm | Needleman-Wunsch |
9124 | | Similarity matrix | BLOSUM-62 |
9125 | | SS fraction | 0.3 |
9126 | | Gap open (HH/SS/other) | 18/18/6 |
9127 | | Gap extend | 1 |
9128 | | SS matrix | | | H | S | O |
9129 | | ---|---|---|--- |
9130 | | H | 6 | -9 | -6 |
9131 | | S | | 6 | -6 |
9132 | | O | | | 4 |
9133 | | Iteration cutoff | 2 |
9134 | | |
9135 | | Matchmaker W20_C1_initial_model_no_wat_lig.pdb BA, chain BA (#50.11) with |
9136 | | F_fixed_model_2_F.pdb, chain F (#56), sequence alignment score = 725 |
9137 | | RMSD between 144 pruned atom pairs is 0.244 angstroms; (across all 144 pairs: |
9138 | | 0.244) |
9139 | | |
9140 | | |
9141 | | > hide #56 models |
9142 | | |
9143 | | > hide #51 models |
9144 | | |
9145 | | > show #36 models |
9146 | | |
9147 | | > hide #36 models |
9148 | | |
9149 | | > show #36 models |
9150 | | |
9151 | | > hide #36 models |
9152 | | |
9153 | | > show #36 models |
9154 | | |
9155 | | > hide #36 models |
9156 | | |
9157 | | > show #35 models |
9158 | | |
9159 | | > hide #35 models |
9160 | | |
9161 | | > show #52 models |
9162 | | |
9163 | | > fitmap #52 inMap #2 |
9164 | | |
9165 | | Fit molecule B_fixed_model_2_B.pdb (#52) to map |
9166 | | W20_J3623_consensus_structure.mrc (#2) using 7500 atoms |
9167 | | average map value = 0.316, steps = 88 |
9168 | | shifted from previous position = 2.73 |
9169 | | rotated from previous position = 5.83 degrees |
9170 | | atoms outside contour = 2940, contour level = 0.25746 |
9171 | | |
9172 | | Position of B_fixed_model_2_B.pdb (#52) relative to |
9173 | | W20_J3623_consensus_structure.mrc (#2) coordinates: |
9174 | | Matrix rotation and translation |
9175 | | -0.33624269 -0.92088355 -0.19726715 276.34525406 |
9176 | | -0.77887628 0.15416253 0.60793557 158.89643495 |
9177 | | -0.52942667 0.35806060 -0.76909038 243.62727331 |
9178 | | Axis -0.56887772 0.75621078 0.32330079 |
9179 | | Axis point 214.85544208 0.00000000 65.79075689 |
9180 | | Rotation angle (degrees) 167.31322736 |
9181 | | Shift along axis 41.71742887 |
9182 | | |
9183 | | |
9184 | | > fitmap #50.112 |
9185 | | |
9186 | | Missing required "in_map" argument |
9187 | | |
9188 | | > fitmap #50.11 inMap #2 |
9189 | | |
9190 | | Fit molecule W20_C1_initial_model_no_wat_lig.pdb BA (#50.11) to map |
9191 | | W20_J3623_consensus_structure.mrc (#2) using 1158 atoms |
9192 | | average map value = 0.2425, steps = 216 |
9193 | | shifted from previous position = 24 |
9194 | | rotated from previous position = 39.5 degrees |
9195 | | atoms outside contour = 674, contour level = 0.25746 |
9196 | | |
9197 | | Position of W20_C1_initial_model_no_wat_lig.pdb BA (#50.11) relative to |
9198 | | W20_J3623_consensus_structure.mrc (#2) coordinates: |
9199 | | Matrix rotation and translation |
9200 | | -0.96490741 -0.23836579 -0.11016098 456.74587096 |
9201 | | 0.21274833 -0.46373391 -0.86005174 403.93698444 |
9202 | | 0.15392153 -0.85330687 0.49817221 176.52114975 |
9203 | | Axis 0.01290218 -0.50515953 0.86292954 |
9204 | | Axis point 197.70794601 288.88132577 0.00000000 |
9205 | | Rotation angle (degrees) 164.84772556 |
9206 | | Shift along axis -45.83428628 |
9207 | | |
9208 | | |
9209 | | > show #56 models |
9210 | | |
9211 | | > hide #56 models |
9212 | | |
9213 | | > show #56 models |
9214 | | |
9215 | | > hide #56 models |
9216 | | |
9217 | | > ui tool show Matchmaker |
9218 | | |
9219 | | > matchmaker #50.11 to #56 |
9220 | | |
9221 | | Computing secondary structure |
9222 | | Parameters |
9223 | | --- |
9224 | | Chain pairing | bb |
9225 | | Alignment algorithm | Needleman-Wunsch |
9226 | | Similarity matrix | BLOSUM-62 |
9227 | | SS fraction | 0.3 |
9228 | | Gap open (HH/SS/other) | 18/18/6 |
9229 | | Gap extend | 1 |
9230 | | SS matrix | | | H | S | O |
9231 | | ---|---|---|--- |
9232 | | H | 6 | -9 | -6 |
9233 | | S | | 6 | -6 |
9234 | | O | | | 4 |
9235 | | Iteration cutoff | 2 |
9236 | | |
9237 | | Matchmaker F_fixed_model_2_F.pdb, chain F (#56) with |
9238 | | W20_C1_initial_model_no_wat_lig.pdb BA, chain BA (#50.11), sequence alignment |
9239 | | score = 725 |
9240 | | RMSD between 144 pruned atom pairs is 0.244 angstroms; (across all 144 pairs: |
9241 | | 0.244) |
9242 | | |
9243 | | |
9244 | | > save "/Users/becca/Desktop/Postdoc/COPI notebooks and |
9245 | | > resources/Processing/W20_MM_cyto_COPI/20250704_W20_COPI_cyto_alignments.cxs" |
9246 | | |
9247 | | > hide #!2 models |
9248 | | |
9249 | | > hide #!50 models |
9250 | | |
9251 | | > show #!50 models |
9252 | | |
9253 | | > hide #52 models |
9254 | | |
9255 | | > show #!53 models |
9256 | | |
9257 | | > hide #!53 models |
9258 | | |
9259 | | > show #!54 models |
9260 | | |
9261 | | > hide #!54 models |
9262 | | |
9263 | | > show #!54 models |
9264 | | |
9265 | | > hide #!54 models |
9266 | | |
9267 | | > show #!55 models |
9268 | | |
9269 | | > hide #!55 models |
9270 | | |
9271 | | > show #52 models |
9272 | | |
9273 | | > show #!33 models |
9274 | | |
9275 | | > color #33 #b2b2b290 models |
9276 | | |
9277 | | > color #33 #b2b2b28f models |
9278 | | |
9279 | | > hide #52 models |
9280 | | |
9281 | | > show #52 models |
9282 | | |
9283 | | > hide #!50.8 models |
9284 | | |
9285 | | > show #!50.8 models |
9286 | | |
9287 | | > hide #50.6 models |
9288 | | |
9289 | | > show #50.6 models |
9290 | | |
9291 | | > hide #!50.4 models |
9292 | | |
9293 | | > hide #!50.8 models |
9294 | | |
9295 | | > show #!50.8 models |
9296 | | |
9297 | | > fitmap #50.8 inMap #2 |
9298 | | |
9299 | | Fit molecule W20_C1_initial_model_no_wat_lig.pdb A7 (#50.8) to map |
9300 | | W20_J3623_consensus_structure.mrc (#2) using 1057 atoms |
9301 | | average map value = 0.2692, steps = 56 |
9302 | | shifted from previous position = 0.944 |
9303 | | rotated from previous position = 4.88 degrees |
9304 | | atoms outside contour = 542, contour level = 0.25746 |
9305 | | |
9306 | | Position of W20_C1_initial_model_no_wat_lig.pdb A7 (#50.8) relative to |
9307 | | W20_J3623_consensus_structure.mrc (#2) coordinates: |
9308 | | Matrix rotation and translation |
9309 | | -0.93105828 0.16643564 -0.32469934 390.48149308 |
9310 | | 0.06181231 -0.80509185 -0.58992064 497.62568127 |
9311 | | -0.35959661 -0.56932091 0.73929966 170.58533102 |
9312 | | Axis 0.18360289 0.31103515 -0.93249510 |
9313 | | Axis point 219.20897097 271.57117220 0.00000000 |
9314 | | Rotation angle (degrees) 176.78409911 |
9315 | | Shift along axis 67.40262593 |
9316 | | |
9317 | | Must specify one map, got 4 |
9318 | | |
9319 | | > hide #!50.8 models |
9320 | | |
9321 | | > show #!50.8 models |
9322 | | |
9323 | | > hide #!33 models |
9324 | | |
9325 | | > show #!46 models |
9326 | | |
9327 | | > show #!33 models |
9328 | | |
9329 | | > hide #!46 models |
9330 | | |
9331 | | > hide #!33.4 models |
9332 | | |
9333 | | > show #!33.4 models |
9334 | | |
9335 | | > hide #!33.3 models |
9336 | | |
9337 | | > show #!33.3 models |
9338 | | |
9339 | | > hide #!33.2 models |
9340 | | |
9341 | | > show #!33.2 models |
9342 | | |
9343 | | > hide #!33.1 models |
9344 | | |
9345 | | > show #!33.1 models |
9346 | | |
9347 | | > fitmap #50.8 inMap #33.1 |
9348 | | |
9349 | | Fit molecule W20_C1_initial_model_no_wat_lig.pdb A7 (#50.8) to map |
9350 | | W20_J160_run_ct6_body001.mrc (#33.1) using 1057 atoms |
9351 | | average map value = 0.01137, steps = 52 |
9352 | | shifted from previous position = 0.606 |
9353 | | rotated from previous position = 3.76 degrees |
9354 | | atoms outside contour = 692, contour level = 0.014811 |
9355 | | |
9356 | | Position of W20_C1_initial_model_no_wat_lig.pdb A7 (#50.8) relative to |
9357 | | W20_J160_run_ct6_body001.mrc (#33.1) coordinates: |
9358 | | Matrix rotation and translation |
9359 | | -0.93056714 0.15394973 -0.33218109 390.44774590 |
9360 | | 0.14816644 -0.67132819 -0.72619912 477.44064772 |
9361 | | -0.33480069 -0.72499513 0.60190577 233.60614941 |
9362 | | Axis 0.18631674 0.40538097 -0.89495941 |
9363 | | Axis point 219.95045580 291.28468198 0.00000000 |
9364 | | Rotation angle (degrees) 179.81487490 |
9365 | | Shift along axis 57.22428050 |
9366 | | |
9367 | | |
9368 | | > hide #!50.8 models |
9369 | | |
9370 | | > show #!50.8 models |
9371 | | |
9372 | | > hide #50.9 models |
9373 | | |
9374 | | > show #50.9 models |
9375 | | |
9376 | | > hide #!50.8 models |
9377 | | |
9378 | | > show #!50.8 models |
9379 | | |
9380 | | > select add #50.8 |
9381 | | |
9382 | | 1057 atoms, 1072 bonds, 1 pseudobond, 131 residues, 2 models selected |
9383 | | |
9384 | | > view matrix models |
9385 | | > #50.8,-0.90846,0.21715,-0.35712,384.12,0.037606,-0.80851,-0.58728,501.46,-0.41627,-0.54695,0.72634,175.24 |
9386 | | |
9387 | | > ui mousemode right "rotate selected models" |
9388 | | |
9389 | | > view matrix models |
9390 | | > #50.8,-0.98593,-0.16588,0.020652,396.52,0.059686,-0.46474,-0.88344,489.6,0.15614,-0.86977,0.4681,195.56 |
9391 | | |
9392 | | > fitmap #50.8 inMap #33.1 |
9393 | | |
9394 | | Fit molecule W20_C1_initial_model_no_wat_lig.pdb A7 (#50.8) to map |
9395 | | W20_J160_run_ct6_body001.mrc (#33.1) using 1057 atoms |
9396 | | average map value = 0.01486, steps = 88 |
9397 | | shifted from previous position = 2.12 |
9398 | | rotated from previous position = 17.4 degrees |
9399 | | atoms outside contour = 497, contour level = 0.014811 |
9400 | | |
9401 | | Position of W20_C1_initial_model_no_wat_lig.pdb A7 (#50.8) relative to |
9402 | | W20_J160_run_ct6_body001.mrc (#33.1) coordinates: |
9403 | | Matrix rotation and translation |
9404 | | -0.98685430 0.03145837 0.15852120 311.01536433 |
9405 | | -0.16062302 -0.29929277 -0.94054457 509.24173521 |
9406 | | 0.01785625 -0.95364260 0.30041129 284.21437212 |
9407 | | Axis -0.05493227 0.58993953 -0.80557668 |
9408 | | Axis point 163.53469015 347.02960176 0.00000000 |
9409 | | Rotation angle (degrees) 173.15292285 |
9410 | | Shift along axis 54.38058021 |
9411 | | |
9412 | | |
9413 | | > select subtract #50.8 |
9414 | | |
9415 | | Nothing selected |
9416 | | |
9417 | | > fitmap #50.7 inMap #33.1 |
9418 | | |
9419 | | Fit molecule W20_C1_initial_model_no_wat_lig.pdb A6 (#50.7) to map |
9420 | | W20_J160_run_ct6_body001.mrc (#33.1) using 4264 atoms |
9421 | | average map value = 0.005768, steps = 160 |
9422 | | shifted from previous position = 2.43 |
9423 | | rotated from previous position = 3.77 degrees |
9424 | | atoms outside contour = 3553, contour level = 0.014811 |
9425 | | |
9426 | | Position of W20_C1_initial_model_no_wat_lig.pdb A6 (#50.7) relative to |
9427 | | W20_J160_run_ct6_body001.mrc (#33.1) coordinates: |
9428 | | Matrix rotation and translation |
9429 | | -0.95014016 0.15038437 -0.27316336 382.66549579 |
9430 | | 0.08966442 -0.70725915 -0.70124517 487.55197868 |
9431 | | -0.29865360 -0.69077423 0.65851119 202.15694868 |
9432 | | Axis 0.15703122 0.38227356 -0.91060865 |
9433 | | Axis point 213.00898151 282.79480162 0.00000000 |
9434 | | Rotation angle (degrees) 178.08938676 |
9435 | | Shift along axis 62.38279471 |
9436 | | |
9437 | | |
9438 | | > hide #50.11 models |
9439 | | |
9440 | | > show #50.11 models |
9441 | | |
9442 | | > fitmap #50.11inMap #33.3 |
9443 | | |
9444 | | Missing or invalid "atomsOrMap" argument: only initial part "#50.11" of atom |
9445 | | specifier valid |
9446 | | |
9447 | | > fitmap #50.11 inMap #33.3 |
9448 | | |
9449 | | Fit molecule W20_C1_initial_model_no_wat_lig.pdb BA (#50.11) to map |
9450 | | W20_J160_run_ct6_body003.mrc (#33.3) using 1158 atoms |
9451 | | average map value = 0.0132, steps = 104 |
9452 | | shifted from previous position = 5.13 |
9453 | | rotated from previous position = 28.7 degrees |
9454 | | atoms outside contour = 711, contour level = 0.015167 |
9455 | | |
9456 | | Position of W20_C1_initial_model_no_wat_lig.pdb BA (#50.11) relative to |
9457 | | W20_J160_run_ct6_body003.mrc (#33.3) coordinates: |
9458 | | Matrix rotation and translation |
9459 | | -0.92321344 -0.13158780 -0.36105621 489.23124927 |
9460 | | 0.28319718 -0.86806041 -0.40776278 396.62096334 |
9461 | | -0.25976200 -0.47870218 0.83867033 84.55689237 |
9462 | | Axis -0.16389770 -0.23402916 0.95831513 |
9463 | | Axis point 230.41567989 235.72565549 0.00000000 |
9464 | | Rotation angle (degrees) 167.50152307 |
9465 | | Shift along axis -91.97259645 |
9466 | | |
9467 | | |
9468 | | > hide #50.11 models |
9469 | | |
9470 | | > show #39 models |
9471 | | |
9472 | | > fitmap #39 inMap #33.3 |
9473 | | |
9474 | | Fit molecule CopZ1_P61924.pdb (#39) to map W20_J160_run_ct6_body003.mrc |
9475 | | (#33.3) using 1420 atoms |
9476 | | average map value = 0.01141, steps = 92 |
9477 | | shifted from previous position = 7.91 |
9478 | | rotated from previous position = 10.3 degrees |
9479 | | atoms outside contour = 963, contour level = 0.015167 |
9480 | | |
9481 | | Position of CopZ1_P61924.pdb (#39) relative to W20_J160_run_ct6_body003.mrc |
9482 | | (#33.3) coordinates: |
9483 | | Matrix rotation and translation |
9484 | | -0.50106153 0.84740615 0.17561366 128.97282199 |
9485 | | -0.08865073 -0.25211715 0.96362752 130.68154277 |
9486 | | 0.86085911 0.46726840 0.20144938 92.25448210 |
9487 | | Axis -0.39337422 -0.54307030 -0.74184323 |
9488 | | Axis point 46.59255051 18.60088904 0.00000000 |
9489 | | Rotation angle (degrees) 140.88349075 |
9490 | | Shift along axis -190.14221075 |
9491 | | |
9492 | | |
9493 | | > select add #39 |
9494 | | |
9495 | | 1420 atoms, 1441 bonds, 177 residues, 1 model selected |
9496 | | |
9497 | | > view matrix models |
9498 | | > #39,-0.038392,0.25438,0.96634,124.66,0.50314,-0.8306,0.23864,135.53,0.86335,0.49537,-0.096103,99.479 |
9499 | | |
9500 | | > fitmap #39 inMap #33.3 |
9501 | | |
9502 | | Fit molecule CopZ1_P61924.pdb (#39) to map W20_J160_run_ct6_body003.mrc |
9503 | | (#33.3) using 1420 atoms |
9504 | | average map value = 0.0138, steps = 140 |
9505 | | shifted from previous position = 3.19 |
9506 | | rotated from previous position = 29.8 degrees |
9507 | | atoms outside contour = 768, contour level = 0.015167 |
9508 | | |
9509 | | Position of CopZ1_P61924.pdb (#39) relative to W20_J160_run_ct6_body003.mrc |
9510 | | (#33.3) coordinates: |
9511 | | Matrix rotation and translation |
9512 | | -0.59910220 0.35688559 0.71673513 128.54412804 |
9513 | | 0.33901457 -0.69789676 0.63087974 127.78779026 |
9514 | | 0.72535901 0.62094508 0.29712207 93.14122293 |
9515 | | Axis -0.44768773 -0.38862007 -0.80532611 |
9516 | | Axis point 38.57930905 41.25889004 0.00000000 |
9517 | | Rotation angle (degrees) 179.36426033 |
9518 | | Shift along axis -182.21758707 |
9519 | | |
9520 | | |
9521 | | > select subtract #39 |
9522 | | |
9523 | | Nothing selected |
9524 | | |
9525 | | > show #!34 models |
9526 | | |
9527 | | > hide #!34 models |
9528 | | |
9529 | | > show #!34 models |
9530 | | |
9531 | | > hide #!34 models |
9532 | | |
9533 | | > hide #39 models |
9534 | | |
9535 | | > hide #!50 models |
9536 | | |
9537 | | > show #!50 models |
9538 | | |
9539 | | > hide #!50 models |
9540 | | |
9541 | | > hide #50.1 models |
9542 | | |
9543 | | > show #!50 models |
9544 | | |
9545 | | > hide #!50 models |
9546 | | |
9547 | | > hide #52 models |
9548 | | |
9549 | | > show #!34 models |
9550 | | |
9551 | | > fitmap #33 inMap #33.3 |
9552 | | |
9553 | | Multiple maps for #33 |
9554 | | |
9555 | | > fitmap #34 inMap #33.2 |
9556 | | |
9557 | | Fit molecule CopA-F8WHL2.pdb (#34) to map W20_J160_run_ct6_body002.mrc (#33.2) |
9558 | | using 6696 atoms |
9559 | | average map value = 0.01229, steps = 144 |
9560 | | shifted from previous position = 0.768 |
9561 | | rotated from previous position = 0.619 degrees |
9562 | | atoms outside contour = 4035, contour level = 0.014809 |
9563 | | |
9564 | | Position of CopA-F8WHL2.pdb (#34) relative to W20_J160_run_ct6_body002.mrc |
9565 | | (#33.2) coordinates: |
9566 | | Matrix rotation and translation |
9567 | | -0.57247775 -0.57456895 -0.58492714 151.44699314 |
9568 | | 0.70447239 -0.70969024 0.00764404 125.42515832 |
9569 | | -0.41950911 -0.40768897 0.81104982 121.20707602 |
9570 | | Axis -0.30653667 -0.12208683 0.94399686 |
9571 | | Axis point 68.14146734 105.20078411 -0.00000000 |
9572 | | Rotation angle (degrees) 137.35447863 |
9573 | | Shift along axis 52.68228363 |
9574 | | |
9575 | | |
9576 | | > show #50.1 models |
9577 | | |
9578 | | > hide #50.1 models |
9579 | | |
9580 | | > show #50.1 models |
9581 | | |
9582 | | > hide #!50 models |
9583 | | |
9584 | | > hide #50.1 models |
9585 | | |
9586 | | > show #!50 models |
9587 | | |
9588 | | > show #50.1 models |
9589 | | |
9590 | | > hide #!50.3 models |
9591 | | |
9592 | | > show #!50.3 models |
9593 | | |
9594 | | > hide #!50.5 models |
9595 | | |
9596 | | > hide #!50.2 models |
9597 | | |
9598 | | > hide #!50.3 models |
9599 | | |
9600 | | > hide #50.6 models |
9601 | | |
9602 | | > hide #!50.8 models |
9603 | | |
9604 | | > hide #50.9 models |
9605 | | |
9606 | | > hide #!50.7 models |
9607 | | |
9608 | | > hide #!50.10 models |
9609 | | |
9610 | | > hide #50.1 models |
9611 | | |
9612 | | > show #!50.2 models |
9613 | | |
9614 | | > show #!50.3 models |
9615 | | |
9616 | | > fitmap #50.2 inMap #33.2 |
9617 | | |
9618 | | Fit molecule W20_C1_initial_model_no_wat_lig.pdb A1 (#50.2) to map |
9619 | | W20_J160_run_ct6_body002.mrc (#33.2) using 2510 atoms |
9620 | | average map value = 0.01804, steps = 44 |
9621 | | shifted from previous position = 0.476 |
9622 | | rotated from previous position = 0.223 degrees |
9623 | | atoms outside contour = 1048, contour level = 0.014809 |
9624 | | |
9625 | | Position of W20_C1_initial_model_no_wat_lig.pdb A1 (#50.2) relative to |
9626 | | W20_J160_run_ct6_body002.mrc (#33.2) coordinates: |
9627 | | Matrix rotation and translation |
9628 | | -0.95004228 0.18207545 -0.25351172 368.13646707 |
9629 | | 0.07472194 -0.65592625 -0.75111742 494.19899480 |
9630 | | -0.30304504 -0.73253620 0.60955264 227.59862474 |
9631 | | Axis 0.15525602 0.41387719 -0.89699567 |
9632 | | Axis point 211.61727753 293.61074195 0.00000000 |
9633 | | Rotation angle (degrees) 176.56933670 |
9634 | | Shift along axis 57.53811301 |
9635 | | |
9636 | | |
9637 | | > fitmap #50.3 inMap #33.2 |
9638 | | |
9639 | | Fit molecule W20_C1_initial_model_no_wat_lig.pdb A2 (#50.3) to map |
9640 | | W20_J160_run_ct6_body002.mrc (#33.2) using 3559 atoms |
9641 | | average map value = 0.01022, steps = 184 |
9642 | | shifted from previous position = 2.7 |
9643 | | rotated from previous position = 7.42 degrees |
9644 | | atoms outside contour = 2357, contour level = 0.014809 |
9645 | | |
9646 | | Position of W20_C1_initial_model_no_wat_lig.pdb A2 (#50.3) relative to |
9647 | | W20_J160_run_ct6_body002.mrc (#33.2) coordinates: |
9648 | | Matrix rotation and translation |
9649 | | -0.93274721 0.18386833 -0.31012106 379.03416947 |
9650 | | 0.07603460 -0.74050966 -0.66773063 496.69481023 |
9651 | | -0.35242216 -0.64640381 0.67672796 193.41619316 |
9652 | | Axis 0.18107201 0.35915085 -0.91554552 |
9653 | | Axis point 216.23338293 280.38752423 0.00000000 |
9654 | | Rotation angle (degrees) 176.62387365 |
9655 | | Shift along axis 69.93951212 |
9656 | | |
9657 | | |
9658 | | > hide #!33 models |
9659 | | |
9660 | | > hide #!34 models |
9661 | | |
9662 | | > show #!34 models |
9663 | | |
9664 | | > hide #!50.2 models |
9665 | | |
9666 | | > show #!50.2 models |
9667 | | |
9668 | | > hide #!50.2 models |
9669 | | |
9670 | | > ui mousemode right select |
9671 | | |
9672 | | Drag select of 223 residues |
9673 | | |
9674 | | > select up |
9675 | | |
9676 | | 2109 atoms, 2166 bonds, 262 residues, 1 model selected |
9677 | | |
9678 | | > select up |
9679 | | |
9680 | | 6696 atoms, 6852 bonds, 834 residues, 1 model selected |
9681 | | |
9682 | | > select down |
9683 | | |
9684 | | 2109 atoms, 2166 bonds, 262 residues, 1 model selected |
9685 | | |
9686 | | > select #34/A:127 |
9687 | | |
9688 | | 6 atoms, 5 bonds, 1 residue, 1 model selected |
9689 | | |
9690 | | > select clear |
9691 | | |
9692 | | Drag select of 1 residues |
9693 | | |
9694 | | > select up |
9695 | | |
9696 | | 6696 atoms, 6852 bonds, 834 residues, 1 model selected |
9697 | | |
9698 | | > select down |
9699 | | |
9700 | | 8 atoms, 1 residue, 1 model selected |
9701 | | Drag select of 190 residues |
9702 | | |
9703 | | > select up |
9704 | | |
9705 | | 1664 atoms, 1710 bonds, 206 residues, 1 model selected |
9706 | | |
9707 | | > select up |
9708 | | |
9709 | | 6696 atoms, 6852 bonds, 834 residues, 1 model selected |
9710 | | |
9711 | | > select down |
9712 | | |
9713 | | 1664 atoms, 1710 bonds, 206 residues, 1 model selected |
9714 | | |
9715 | | > delete sel |
9716 | | |
9717 | | Drag select of 63 residues, 2 pseudobonds |
9718 | | |
9719 | | > select up |
9720 | | |
9721 | | 788 atoms, 803 bonds, 2 pseudobonds, 96 residues, 2 models selected |
9722 | | |
9723 | | > delete sel |
9724 | | |
9725 | | Drag select of 19 residues, 2 pseudobonds |
9726 | | |
9727 | | > delete sel |
9728 | | |
9729 | | Drag select of 4 residues |
9730 | | |
9731 | | > delete sel |
9732 | | |
9733 | | > show #!50.2 models |
9734 | | |
9735 | | > show #!33 models |
9736 | | |
9737 | | > fitmap #34 inMap #33.2 |
9738 | | |
9739 | | Fit molecule CopA-F8WHL2.pdb (#34) to map W20_J160_run_ct6_body002.mrc (#33.2) |
9740 | | using 4046 atoms |
9741 | | average map value = 0.009675, steps = 164 |
9742 | | shifted from previous position = 0.697 |
9743 | | rotated from previous position = 2.08 degrees |
9744 | | atoms outside contour = 2782, contour level = 0.014809 |
9745 | | |
9746 | | Position of CopA-F8WHL2.pdb (#34) relative to W20_J160_run_ct6_body002.mrc |
9747 | | (#33.2) coordinates: |
9748 | | Matrix rotation and translation |
9749 | | -0.58668811 -0.55339986 -0.59122387 151.25489618 |
9750 | | 0.67908972 -0.73393831 0.01310416 124.24263215 |
9751 | | -0.44117369 -0.39380599 0.80640103 120.81607944 |
9752 | | Axis -0.31143421 -0.11484294 0.94330262 |
9753 | | Axis point 69.89652487 102.51139715 0.00000000 |
9754 | | Rotation angle (degrees) 139.21031634 |
9755 | | Shift along axis 52.59178621 |
9756 | | |
9757 | | |
9758 | | > fitmap #50.3 inMap #33.2 |
9759 | | |
9760 | | Fit molecule W20_C1_initial_model_no_wat_lig.pdb A2 (#50.3) to map |
9761 | | W20_J160_run_ct6_body002.mrc (#33.2) using 3559 atoms |
9762 | | average map value = 0.01022, steps = 160 |
9763 | | shifted from previous position = 0.0132 |
9764 | | rotated from previous position = 0.015 degrees |
9765 | | atoms outside contour = 2353, contour level = 0.014809 |
9766 | | |
9767 | | Position of W20_C1_initial_model_no_wat_lig.pdb A2 (#50.3) relative to |
9768 | | W20_J160_run_ct6_body002.mrc (#33.2) coordinates: |
9769 | | Matrix rotation and translation |
9770 | | -0.93280742 0.18368258 -0.31005003 379.08438259 |
9771 | | 0.07605879 -0.74062741 -0.66759726 496.69733667 |
9772 | | -0.35225754 -0.64632171 0.67689207 193.32199255 |
9773 | | Axis 0.18099805 0.35907301 -0.91559067 |
9774 | | Axis point 216.22488098 280.37147530 0.00000000 |
9775 | | Rotation angle (degrees) 176.63062081 |
9776 | | Shift along axis 69.96032790 |
9777 | | |
9778 | | |
9779 | | > combine #34 |
9780 | | |
9781 | | > combine #50.3 |
9782 | | |
9783 | | > hide #!50.3 models |
9784 | | |
9785 | | > hide #!34 models |
9786 | | |
9787 | | > hide #!33.4 models |
9788 | | |
9789 | | > show #!33.4 models |
9790 | | |
9791 | | > hide #!33 models |
9792 | | |
9793 | | > hide #!58 models |
9794 | | |
9795 | | > select clear |
9796 | | |
9797 | | > hide #!50.2 models |
9798 | | |
9799 | | Drag select of 243 residues |
9800 | | |
9801 | | > select up |
9802 | | |
9803 | | 2056 atoms, 2095 bonds, 259 residues, 1 model selected |
9804 | | |
9805 | | > select up |
9806 | | |
9807 | | 4046 atoms, 4125 bonds, 509 residues, 1 model selected |
9808 | | |
9809 | | > select up |
9810 | | |
9811 | | 101979 atoms, 103831 bonds, 12855 residues, 91 models selected |
9812 | | |
9813 | | > select clear |
9814 | | |
9815 | | Drag select of 274 residues |
9816 | | |
9817 | | > select up |
9818 | | |
9819 | | 2192 atoms, 2235 bonds, 276 residues, 1 model selected |
9820 | | |
9821 | | > delete sel |
9822 | | |
9823 | | Drag select of 2 residues, 1 pseudobonds |
9824 | | |
9825 | | > delete sel |
9826 | | |
9827 | | > show #!33 models |
9828 | | |
9829 | | > fitmap #57 inMap #33.1 |
9830 | | |
9831 | | Fit molecule copy of CopA-F8WHL2.pdb (#57) to map W20_J160_run_ct6_body001.mrc |
9832 | | (#33.1) using 1842 atoms |
9833 | | average map value = 0.01399, steps = 76 |
9834 | | shifted from previous position = 7.58 |
9835 | | rotated from previous position = 11.9 degrees |
9836 | | atoms outside contour = 1005, contour level = 0.014811 |
9837 | | |
9838 | | Position of copy of CopA-F8WHL2.pdb (#57) relative to |
9839 | | W20_J160_run_ct6_body001.mrc (#33.1) coordinates: |
9840 | | Matrix rotation and translation |
9841 | | -0.66070189 -0.61639558 -0.42840344 148.88789464 |
9842 | | 0.70166098 -0.70992235 -0.06068050 124.32932168 |
9843 | | -0.26672999 -0.34068570 0.90154776 127.22528478 |
9844 | | Axis -0.20632088 -0.11912853 0.97120548 |
9845 | | Axis point 61.04505270 103.19655495 0.00000000 |
9846 | | Rotation angle (degrees) 137.26821223 |
9847 | | Shift along axis 78.03204323 |
9848 | | |
9849 | | |
9850 | | > hide #!57 models |
9851 | | |
9852 | | > hide #!33.4 models |
9853 | | |
9854 | | > show #!33.4 models |
9855 | | |
9856 | | > hide #!33 models |
9857 | | |
9858 | | > show #!58 models |
9859 | | |
9860 | | Drag select of 104 residues, 1 pseudobonds |
9861 | | |
9862 | | > select clear |
9863 | | |
9864 | | Drag select of 282 residues, 6 pseudobonds |
9865 | | |
9866 | | > delete sel |
9867 | | |
9868 | | > fitmap #87 inMap #33.1 |
9869 | | |
9870 | | No atoms or maps for #87 |
9871 | | |
9872 | | > fitmap #58 inMap #33.1 |
9873 | | |
9874 | | Fit molecule copy of W20_C1_initial_model_no_wat_lig.pdb A2 (#58) to map |
9875 | | W20_J160_run_ct6_body001.mrc (#33.1) using 1297 atoms |
9876 | | average map value = 0.01501, steps = 108 |
9877 | | shifted from previous position = 10.8 |
9878 | | rotated from previous position = 12.4 degrees |
9879 | | atoms outside contour = 664, contour level = 0.014811 |
9880 | | |
9881 | | Position of copy of W20_C1_initial_model_no_wat_lig.pdb A2 (#58) relative to |
9882 | | W20_J160_run_ct6_body001.mrc (#33.1) coordinates: |
9883 | | Matrix rotation and translation |
9884 | | -0.95774575 0.09731996 -0.27065088 396.29244001 |
9885 | | 0.15737130 -0.61033953 -0.77635039 474.71597461 |
9886 | | -0.24074332 -0.78613897 0.56923472 242.75449330 |
9887 | | Axis -0.14438058 -0.44113353 0.88575135 |
9888 | | Axis point 213.60576536 301.52927607 0.00000000 |
9889 | | Rotation angle (degrees) 178.05738481 |
9890 | | Shift along axis -51.60994703 |
9891 | | |
9892 | | |
9893 | | > show #!33 models |
9894 | | |
9895 | | > show #!57 models |
9896 | | |
9897 | | > hide #!58 models |
9898 | | |
9899 | | > show #!58 models |
9900 | | |
9901 | | > hide #!57 models |
9902 | | |
9903 | | > show #!57 models |
9904 | | |
9905 | | > hide #!33 models |
9906 | | |
9907 | | > show #!34 models |
9908 | | |
9909 | | > hide #!57 models |
9910 | | |
9911 | | > hide #!58 models |
9912 | | |
9913 | | > show #!58 models |
9914 | | |
9915 | | > hide #!58 models |
9916 | | |
9917 | | > show #!58 models |
9918 | | |
9919 | | > hide #!58 models |
9920 | | |
9921 | | Drag select of 144 residues |
9922 | | |
9923 | | > select clear |
9924 | | |
9925 | | [Repeated 1 time(s)] |
9926 | | |
9927 | | > combine #34 |
9928 | | |
9929 | | > hide #!34 models |
9930 | | |
9931 | | > show #!58 models |
9932 | | |
9933 | | > hide #!58 models |
9934 | | |
9935 | | > show #!58 models |
9936 | | |
9937 | | > hide #!58 models |
9938 | | |
9939 | | > show #!58 models |
9940 | | |
9941 | | > hide #!58 models |
9942 | | |
9943 | | > show #!58 models |
9944 | | |
9945 | | > hide #!58 models |
9946 | | |
9947 | | Drag select of 161 residues |
9948 | | |
9949 | | > select up |
9950 | | |
9951 | | 1359 atoms, 1382 bonds, 172 residues, 1 model selected |
9952 | | |
9953 | | > show #!58 models |
9954 | | |
9955 | | > hide #!58 models |
9956 | | |
9957 | | > delete sel |
9958 | | |
9959 | | Drag select of 9 residues |
9960 | | |
9961 | | > delete sel |
9962 | | |
9963 | | > fitmap #59 inMap #33.1 |
9964 | | |
9965 | | Fit molecule copy of CopA-F8WHL2.pdb (#59) to map W20_J160_run_ct6_body001.mrc |
9966 | | (#33.1) using 2618 atoms |
9967 | | average map value = 0.00517, steps = 108 |
9968 | | shifted from previous position = 1.61 |
9969 | | rotated from previous position = 4.74 degrees |
9970 | | atoms outside contour = 2287, contour level = 0.014811 |
9971 | | |
9972 | | Position of copy of CopA-F8WHL2.pdb (#59) relative to |
9973 | | W20_J160_run_ct6_body001.mrc (#33.1) coordinates: |
9974 | | Matrix rotation and translation |
9975 | | -0.61097397 -0.53341027 -0.58496522 151.41100776 |
9976 | | 0.68780030 -0.72352924 -0.05861913 123.94831007 |
9977 | | -0.39197139 -0.43815401 0.80893726 122.28836749 |
9978 | | Axis -0.29346029 -0.14922482 0.94425262 |
9979 | | Axis point 69.10859861 104.04335802 0.00000000 |
9980 | | Rotation angle (degrees) 139.71015631 |
9981 | | Shift along axis 52.54182884 |
9982 | | |
9983 | | |
9984 | | > fitmap #59 inMap #33.2 |
9985 | | |
9986 | | Fit molecule copy of CopA-F8WHL2.pdb (#59) to map W20_J160_run_ct6_body002.mrc |
9987 | | (#33.2) using 2618 atoms |
9988 | | average map value = 0.0148, steps = 72 |
9989 | | shifted from previous position = 1.57 |
9990 | | rotated from previous position = 4.66 degrees |
9991 | | atoms outside contour = 1354, contour level = 0.014809 |
9992 | | |
9993 | | Position of copy of CopA-F8WHL2.pdb (#59) relative to |
9994 | | W20_J160_run_ct6_body002.mrc (#33.2) coordinates: |
9995 | | Matrix rotation and translation |
9996 | | -0.58463217 -0.55176650 -0.59477640 151.19300478 |
9997 | | 0.68006639 -0.73305905 0.01158206 124.19731133 |
9998 | | -0.44239681 -0.39771619 0.80380775 120.82904766 |
9999 | | Axis -0.31316702 -0.11659043 0.94251424 |
10000 | | Axis point 69.96688260 102.62111999 0.00000000 |
10001 | | Rotation angle (degrees) 139.19532441 |
10002 | | Shift along axis 52.05421740 |
10003 | | |
10004 | | |
10005 | | > show #!33 models |
10006 | | |
10007 | | > show #!58 models |
10008 | | |
10009 | | > show #!50.2 models |
10010 | | |
10011 | | > hide #!33 models |
10012 | | |
10013 | | > show #!34 models |
10014 | | |
10015 | | > hide #!34 models |
10016 | | |
10017 | | > show #!34 models |
10018 | | |
10019 | | > hide #!34 models |
10020 | | |
10021 | | > show #!34 models |
10022 | | |
10023 | | > hide #!34 models |
10024 | | |
10025 | | > show #!34 models |
10026 | | |
10027 | | > hide #!50.2 models |
10028 | | |
10029 | | > show #!50.2 models |
10030 | | |
10031 | | > hide #!34 models |
10032 | | |
10033 | | > show #!34 models |
10034 | | |
10035 | | > hide #!34 models |
10036 | | |
10037 | | > show #!34 models |
10038 | | |
10039 | | > hide #!34 models |
10040 | | |
10041 | | > hide #!59 models |
10042 | | |
10043 | | > show #!59 models |
10044 | | |
10045 | | > show #!57 models |
10046 | | |
10047 | | > hide #!57 models |
10048 | | |
10049 | | > show #!57 models |
10050 | | |
10051 | | > hide #!57 models |
10052 | | |
10053 | | > hide #!59 models |
10054 | | |
10055 | | > show #!57 models |
10056 | | |
10057 | | > hide #!58 models |
10058 | | |
10059 | | > hide #!57 models |
10060 | | |
10061 | | > show #!33 models |
10062 | | |
10063 | | > show #!57 models |
10064 | | |
10065 | | > show #!58 models |
10066 | | |
10067 | | > hide #!58 models |
10068 | | |
10069 | | > show #!59 models |
10070 | | |
10071 | | > hide #!59 models |
10072 | | |
10073 | | > show #!59 models |
10074 | | |
10075 | | > hide #!57 models |
10076 | | |
10077 | | > show #!58 models |
10078 | | |
10079 | | > hide #!33 models |
10080 | | |
10081 | | > show #51 models |
10082 | | |
10083 | | > combine #51 |
10084 | | |
10085 | | > hide #51 models |
10086 | | |
10087 | | > hide #!59 models |
10088 | | |
10089 | | > hide #!58 models |
10090 | | |
10091 | | > hide #!50.2 models |
10092 | | |
10093 | | Drag select of 667 residues |
10094 | | |
10095 | | > select up |
10096 | | |
10097 | | 6183 atoms, 6300 bonds, 784 residues, 1 model selected |
10098 | | |
10099 | | > delete sel |
10100 | | |
10101 | | Drag select of 87 residues, 10 pseudobonds |
10102 | | |
10103 | | > select clear |
10104 | | |
10105 | | Drag select of 61 residues, 5 pseudobonds |
10106 | | |
10107 | | > select up |
10108 | | |
10109 | | 758 atoms, 752 bonds, 5 pseudobonds, 95 residues, 2 models selected |
10110 | | |
10111 | | > delete sel |
10112 | | |
10113 | | Drag select of 27 residues, 3 pseudobonds |
10114 | | |
10115 | | > select up |
10116 | | |
10117 | | 2800 atoms, 2874 bonds, 3 pseudobonds, 345 residues, 2 models selected |
10118 | | |
10119 | | > select down |
10120 | | |
10121 | | 210 atoms, 3 pseudobonds, 27 residues, 2 models selected |
10122 | | |
10123 | | > delete sel |
10124 | | |
10125 | | > fitmap #60 inMap #33.2 |
10126 | | |
10127 | | Fit molecule copy of A_fixed_model_2_A.pdb (#60) to map |
10128 | | W20_J160_run_ct6_body002.mrc (#33.2) using 2590 atoms |
10129 | | average map value = 0.01604, steps = 212 |
10130 | | shifted from previous position = 8.36 |
10131 | | rotated from previous position = 37.7 degrees |
10132 | | atoms outside contour = 1297, contour level = 0.014809 |
10133 | | |
10134 | | Position of copy of A_fixed_model_2_A.pdb (#60) relative to |
10135 | | W20_J160_run_ct6_body002.mrc (#33.2) coordinates: |
10136 | | Matrix rotation and translation |
10137 | | -0.12666898 -0.97960527 -0.15597590 240.27472629 |
10138 | | 0.03811656 -0.16193319 0.98606530 35.82542257 |
10139 | | -0.99121244 0.11895862 0.05785109 200.76320490 |
10140 | | Axis -0.55003121 0.52981504 0.64557082 |
10141 | | Axis point 195.41347917 29.75172699 0.00000000 |
10142 | | Rotation angle (degrees) 127.97921254 |
10143 | | Shift along axis 16.42911600 |
10144 | | |
10145 | | |
10146 | | > show #!33 models |
10147 | | |
10148 | | > show #50.1 models |
10149 | | |
10150 | | > hide #50.1 models |
10151 | | |
10152 | | > show #!50.2 models |
10153 | | |
10154 | | > select add #60 |
10155 | | |
10156 | | 2590 atoms, 2665 bonds, 318 residues, 1 model selected |
10157 | | |
10158 | | > ui mousemode right "rotate selected models" |
10159 | | |
10160 | | > view matrix models |
10161 | | > #60,-0.3575,-0.93077,-0.076565,262.87,-0.90319,0.36543,-0.22518,271.82,0.23757,-0.011349,-0.9713,201.17 |
10162 | | |
10163 | | > ui tool show Matchmaker |
10164 | | |
10165 | | > matchmaker #!60 to #50.2 |
10166 | | |
10167 | | Computing secondary structure |
10168 | | Parameters |
10169 | | --- |
10170 | | Chain pairing | bb |
10171 | | Alignment algorithm | Needleman-Wunsch |
10172 | | Similarity matrix | BLOSUM-62 |
10173 | | SS fraction | 0.3 |
10174 | | Gap open (HH/SS/other) | 18/18/6 |
10175 | | Gap extend | 1 |
10176 | | SS matrix | | | H | S | O |
10177 | | ---|---|---|--- |
10178 | | H | 6 | -9 | -6 |
10179 | | S | | 6 | -6 |
10180 | | O | | | 4 |
10181 | | Iteration cutoff | 2 |
10182 | | |
10183 | | Matchmaker W20_C1_initial_model_no_wat_lig.pdb A1, chain A1 (#50.2) with copy |
10184 | | of A_fixed_model_2_A.pdb, chain A (#60), sequence alignment score = 1588.3 |
10185 | | RMSD between 296 pruned atom pairs is 0.254 angstroms; (across all 296 pairs: |
10186 | | 0.254) |
10187 | | |
10188 | | |
10189 | | > hide #!50.2 models |
10190 | | |
10191 | | > hide #!50 models |
10192 | | |
10193 | | > show #!50 models |
10194 | | |
10195 | | > hide #!50 models |
10196 | | |
10197 | | > select subtract #60 |
10198 | | |
10199 | | Nothing selected |
10200 | | |
10201 | | > show #!50.2 models |
10202 | | |
10203 | | > show #51 models |
10204 | | |
10205 | | > hide #51 models |
10206 | | |
10207 | | > show #51 models |
10208 | | |
10209 | | > hide #51 models |
10210 | | |
10211 | | > combine #51 |
10212 | | |
10213 | | > hide #!33 models |
10214 | | |
10215 | | > hide #!50.2 models |
10216 | | |
10217 | | > hide #!60 models |
10218 | | |
10219 | | > ui mousemode right select |
10220 | | |
10221 | | Drag select of 103 residues |
10222 | | |
10223 | | > select up |
10224 | | |
10225 | | 1168 atoms, 1193 bonds, 145 residues, 1 model selected |
10226 | | |
10227 | | > delete sel |
10228 | | |
10229 | | > hide #!61 models |
10230 | | |
10231 | | > show #!60 models |
10232 | | |
10233 | | > hide #!60 models |
10234 | | |
10235 | | > show #!61 models |
10236 | | |
10237 | | > select #61/A:318 |
10238 | | |
10239 | | 9 atoms, 8 bonds, 1 residue, 1 model selected |
10240 | | |
10241 | | > select up |
10242 | | |
10243 | | 47 atoms, 47 bonds, 6 residues, 1 model selected |
10244 | | |
10245 | | > select up |
10246 | | |
10247 | | 7736 atoms, 7896 bonds, 978 residues, 1 model selected |
10248 | | |
10249 | | > select down |
10250 | | |
10251 | | 47 atoms, 47 bonds, 6 residues, 1 model selected |
10252 | | |
10253 | | > delete sel |
10254 | | |
10255 | | Drag select of 100 residues, 3 pseudobonds |
10256 | | |
10257 | | > select up |
10258 | | |
10259 | | 1091 atoms, 1121 bonds, 3 pseudobonds, 134 residues, 2 models selected |
10260 | | |
10261 | | > select down |
10262 | | |
10263 | | 793 atoms, 3 pseudobonds, 100 residues, 2 models selected |
10264 | | |
10265 | | > select clear |
10266 | | |
10267 | | > select #61/A:1 |
10268 | | |
10269 | | 8 atoms, 7 bonds, 1 residue, 1 model selected |
10270 | | |
10271 | | > select up |
10272 | | |
10273 | | 533 atoms, 549 bonds, 64 residues, 1 model selected |
10274 | | |
10275 | | > delete sel |
10276 | | |
10277 | | Drag select of 9 residues, 1 pseudobonds |
10278 | | |
10279 | | > delete sel |
10280 | | |
10281 | | Drag select of 26 residues, 1 pseudobonds |
10282 | | |
10283 | | > select up |
10284 | | |
10285 | | 258 atoms, 264 bonds, 1 pseudobond, 33 residues, 2 models selected |
10286 | | |
10287 | | > delete sel |
10288 | | |
10289 | | Drag select of 7 residues |
10290 | | |
10291 | | > select clear |
10292 | | |
10293 | | [Repeated 1 time(s)]Drag select of 13 residues |
10294 | | Drag select of 48 residues |
10295 | | |
10296 | | > select up |
10297 | | |
10298 | | 507 atoms, 517 bonds, 62 residues, 1 model selected |
10299 | | |
10300 | | > select down |
10301 | | |
10302 | | 384 atoms, 48 residues, 1 model selected |
10303 | | |
10304 | | > select clear |
10305 | | |
10306 | | Drag select of 50 residues |
10307 | | |
10308 | | > select up |
10309 | | |
10310 | | 507 atoms, 517 bonds, 62 residues, 1 model selected |
10311 | | |
10312 | | > select clear |
10313 | | |
10314 | | Drag select of 1 residues |
10315 | | |
10316 | | > select up |
10317 | | |
10318 | | 42 atoms, 42 bonds, 6 residues, 1 model selected |
10319 | | |
10320 | | > delete sel |
10321 | | |
10322 | | > select #61/A:263 |
10323 | | |
10324 | | 8 atoms, 7 bonds, 1 residue, 1 model selected |
10325 | | |
10326 | | > select up |
10327 | | |
10328 | | 44 atoms, 43 bonds, 6 residues, 1 model selected |
10329 | | |
10330 | | > delete sel |
10331 | | |
10332 | | > select clear |
10333 | | |
10334 | | > select #61/A:258 |
10335 | | |
10336 | | 10 atoms, 10 bonds, 1 residue, 1 model selected |
10337 | | |
10338 | | > select clear |
10339 | | |
10340 | | Drag select of 4 residues |
10341 | | |
10342 | | > select clear |
10343 | | |
10344 | | Drag select of 4 residues |
10345 | | |
10346 | | > select clear |
10347 | | |
10348 | | Drag select of 5 residues |
10349 | | |
10350 | | > select up |
10351 | | |
10352 | | 121 atoms, 122 bonds, 14 residues, 1 model selected |
10353 | | |
10354 | | > delete sel |
10355 | | |
10356 | | > select clear |
10357 | | |
10358 | | > select #61/A:273 |
10359 | | |
10360 | | 8 atoms, 7 bonds, 1 residue, 1 model selected |
10361 | | |
10362 | | > select up |
10363 | | |
10364 | | 54 atoms, 55 bonds, 6 residues, 1 model selected |
10365 | | |
10366 | | > delete sel |
10367 | | |
10368 | | > select #61/A:278 |
10369 | | |
10370 | | 8 atoms, 7 bonds, 1 residue, 1 model selected |
10371 | | |
10372 | | > select clear |
10373 | | |
10374 | | Drag select of 1 residues |
10375 | | Drag select of 6 residues |
10376 | | |
10377 | | > select up |
10378 | | |
10379 | | 90 atoms, 90 bonds, 11 residues, 1 model selected |
10380 | | |
10381 | | > fitmap #60 inMap #33.2 |
10382 | | |
10383 | | Fit molecule copy of A_fixed_model_2_A.pdb (#60) to map |
10384 | | W20_J160_run_ct6_body002.mrc (#33.2) using 2590 atoms |
10385 | | average map value = 0.01743, steps = 48 |
10386 | | shifted from previous position = 0.0339 |
10387 | | rotated from previous position = 0.278 degrees |
10388 | | atoms outside contour = 1142, contour level = 0.014809 |
10389 | | |
10390 | | Position of copy of A_fixed_model_2_A.pdb (#60) relative to |
10391 | | W20_J160_run_ct6_body002.mrc (#33.2) coordinates: |
10392 | | Matrix rotation and translation |
10393 | | -0.44176945 -0.87418787 -0.20158210 278.02704219 |
10394 | | -0.65633376 0.16174616 0.73692889 133.70992844 |
10395 | | -0.61160916 0.45785781 -0.64521352 224.98469771 |
10396 | | Axis -0.51515506 0.75689501 0.40215074 |
10397 | | Axis point 203.25110647 0.00000000 49.34812669 |
10398 | | Rotation angle (degrees) 164.28447736 |
10399 | | Shift along axis 48.45510328 |
10400 | | |
10401 | | |
10402 | | > delete sel |
10403 | | |
10404 | | Drag select of 16 residues, 1 pseudobonds |
10405 | | |
10406 | | > select up |
10407 | | |
10408 | | 205 atoms, 209 bonds, 1 pseudobond, 25 residues, 2 models selected |
10409 | | |
10410 | | > select down |
10411 | | |
10412 | | 126 atoms, 1 pseudobond, 16 residues, 2 models selected |
10413 | | |
10414 | | > delete sel |
10415 | | |
10416 | | Drag select of 2 residues |
10417 | | |
10418 | | > delete sel |
10419 | | |
10420 | | > show #!60 models |
10421 | | |
10422 | | > show #!33 models |
10423 | | |
10424 | | > fitmap #61 inMap #33.1 |
10425 | | |
10426 | | Fit molecule copy of A_fixed_model_2_A.pdb (#61) to map |
10427 | | W20_J160_run_ct6_body001.mrc (#33.1) using 7151 atoms |
10428 | | average map value = 0.004441, steps = 228 |
10429 | | shifted from previous position = 5.35 |
10430 | | rotated from previous position = 8.58 degrees |
10431 | | atoms outside contour = 6297, contour level = 0.014811 |
10432 | | |
10433 | | Position of copy of A_fixed_model_2_A.pdb (#61) relative to |
10434 | | W20_J160_run_ct6_body001.mrc (#33.1) coordinates: |
10435 | | Matrix rotation and translation |
10436 | | -0.45222649 -0.82892764 -0.32919625 288.15586650 |
10437 | | -0.57376739 -0.01220253 0.81892739 121.44819917 |
10438 | | -0.68284858 0.55922273 -0.47009335 215.98047971 |
10439 | | Axis -0.51166679 0.69676128 0.50271344 |
10440 | | Axis point 206.53531672 0.00000000 35.33484489 |
10441 | | Rotation angle (degrees) 165.29851626 |
10442 | | Shift along axis 45.75690645 |
10443 | | |
10444 | | |
10445 | | > fitmap #61 inMap #33.2 |
10446 | | |
10447 | | Fit molecule copy of A_fixed_model_2_A.pdb (#61) to map |
10448 | | W20_J160_run_ct6_body002.mrc (#33.2) using 7151 atoms |
10449 | | average map value = 0.005548, steps = 2000 |
10450 | | shifted from previous position = 4.46 |
10451 | | rotated from previous position = 9.55 degrees |
10452 | | atoms outside contour = 5855, contour level = 0.014809 |
10453 | | |
10454 | | Position of copy of A_fixed_model_2_A.pdb (#61) relative to |
10455 | | W20_J160_run_ct6_body002.mrc (#33.2) coordinates: |
10456 | | Matrix rotation and translation |
10457 | | -0.32348189 -0.90777241 -0.26703693 271.47554220 |
10458 | | -0.61054188 -0.01536719 0.79183487 128.17226278 |
10459 | | -0.72290946 0.41918147 -0.54926207 231.58959567 |
10460 | | Axis -0.56499205 0.69116330 0.45064096 |
10461 | | Axis point 214.35725794 0.00000000 37.95145878 |
10462 | | Rotation angle (degrees) 160.74418801 |
10463 | | Shift along axis 39.57019701 |
10464 | | |
10465 | | |
10466 | | > combine #61 |
10467 | | |
10468 | | > hide #!61 models |
10469 | | |
10470 | | > hide #!60 models |
10471 | | |
10472 | | > hide #!50 models |
10473 | | |
10474 | | > show #!50 models |
10475 | | |
10476 | | > hide #!33 models |
10477 | | |
10478 | | Drag select of 1 residues |
10479 | | |
10480 | | > select up |
10481 | | |
10482 | | 7151 atoms, 7294 bonds, 906 residues, 1 model selected |
10483 | | |
10484 | | > select clear |
10485 | | |
10486 | | Drag select of 169 residues |
10487 | | |
10488 | | > select up |
10489 | | |
10490 | | 1541 atoms, 1568 bonds, 193 residues, 1 model selected |
10491 | | |
10492 | | > delete sel |
10493 | | |
10494 | | Drag select of 51 residues, 1 pseudobonds |
10495 | | |
10496 | | > select up |
10497 | | |
10498 | | 646 atoms, 657 bonds, 1 pseudobond, 82 residues, 2 models selected |
10499 | | |
10500 | | > delete sel |
10501 | | |
10502 | | Drag select of 4 residues |
10503 | | |
10504 | | > select clear |
10505 | | |
10506 | | Drag select of 3 residues |
10507 | | |
10508 | | > select clear |
10509 | | |
10510 | | [Repeated 2 time(s)]Drag select of 20 residues, 2 pseudobonds |
10511 | | |
10512 | | > select up |
10513 | | |
10514 | | 796 atoms, 812 bonds, 2 pseudobonds, 108 residues, 2 models selected |
10515 | | |
10516 | | > delete sel |
10517 | | |
10518 | | Drag select of 8 residues, 1 pseudobonds |
10519 | | |
10520 | | > select clear |
10521 | | |
10522 | | Drag select of 15 residues |
10523 | | |
10524 | | > delete sel |
10525 | | |
10526 | | > select clear |
10527 | | |
10528 | | > delete sel |
10529 | | |
10530 | | > select clear |
10531 | | |
10532 | | > delete sel |
10533 | | |
10534 | | > close #62 |
10535 | | |
10536 | | > combine #61 |
10537 | | |
10538 | | Drag select of 269 residues |
10539 | | |
10540 | | > select up |
10541 | | |
10542 | | 2282 atoms, 2326 bonds, 286 residues, 1 model selected |
10543 | | |
10544 | | > delete sel |
10545 | | |
10546 | | Drag select of 37 residues, 2 pseudobonds |
10547 | | |
10548 | | > select up |
10549 | | |
10550 | | 738 atoms, 753 bonds, 2 pseudobonds, 101 residues, 2 models selected |
10551 | | |
10552 | | > delete sel |
10553 | | |
10554 | | Drag select of 10 residues |
10555 | | |
10556 | | > delete sel |
10557 | | |
10558 | | > close #62 |
10559 | | |
10560 | | > combine #61 |
10561 | | |
10562 | | > select clear |
10563 | | |
10564 | | [Repeated 1 time(s)] |
10565 | | |
10566 | | > select #62/A:873 |
10567 | | |
10568 | | 4 atoms, 3 bonds, 1 residue, 1 model selected |
10569 | | |
10570 | | > select clear |
10571 | | |
10572 | | Drag select of 1 residues |
10573 | | |
10574 | | > select up |
10575 | | |
10576 | | 645 atoms, 657 bonds, 90 residues, 1 model selected |
10577 | | |
10578 | | > delete sel |
10579 | | |
10580 | | Drag select of 309 residues |
10581 | | |
10582 | | > delete sel |
10583 | | |
10584 | | > fitmap #62 inMap #33.1 |
10585 | | |
10586 | | Fit molecule copy of copy of A_fixed_model_2_A.pdb (#62) to map |
10587 | | W20_J160_run_ct6_body001.mrc (#33.1) using 4038 atoms |
10588 | | average map value = 0.007876, steps = 128 |
10589 | | shifted from previous position = 3.44 |
10590 | | rotated from previous position = 10.8 degrees |
10591 | | atoms outside contour = 3167, contour level = 0.014811 |
10592 | | |
10593 | | Position of copy of copy of A_fixed_model_2_A.pdb (#62) relative to |
10594 | | W20_J160_run_ct6_body001.mrc (#33.1) coordinates: |
10595 | | Matrix rotation and translation |
10596 | | -0.49233635 -0.83165726 -0.25680949 286.92215484 |
10597 | | -0.53267730 0.05454857 0.84455867 111.52201588 |
10598 | | -0.68837476 0.55260352 -0.46986119 217.04968179 |
10599 | | Axis -0.48600137 0.71840251 0.49769518 |
10600 | | Axis point 201.81367843 0.00000000 36.66306761 |
10601 | | Rotation angle (degrees) 162.52050103 |
10602 | | Shift along axis 48.69771641 |
10603 | | |
10604 | | |
10605 | | > show #!33 models |
10606 | | |
10607 | | > hide #!62 models |
10608 | | |
10609 | | > show #!62 models |
10610 | | |
10611 | | > hide #!62 models |
10612 | | |
10613 | | > show #!60 models |
10614 | | |
10615 | | > show #!61 models |
10616 | | |
10617 | | > hide #!61 models |
10618 | | |
10619 | | > show #!61 models |
10620 | | |
10621 | | > show #!62 models |
10622 | | |
10623 | | > hide #!62 models |
10624 | | |
10625 | | > show #!62 models |
10626 | | |
10627 | | > hide #!62 models |
10628 | | |
10629 | | > close #61 |
10630 | | |
10631 | | > show #!62 models |
10632 | | |
10633 | | > combine #62 |
10634 | | |
10635 | | > hide #!62 models |
10636 | | |
10637 | | > hide #!60 models |
10638 | | |
10639 | | > fitmap #61 inMap #33.2 |
10640 | | |
10641 | | Fit molecule copy of copy of copy of A_fixed_model_2_A.pdb (#61) to map |
10642 | | W20_J160_run_ct6_body002.mrc (#33.2) using 4038 atoms |
10643 | | average map value = 0.009974, steps = 196 |
10644 | | shifted from previous position = 3.26 |
10645 | | rotated from previous position = 10.9 degrees |
10646 | | atoms outside contour = 2744, contour level = 0.014809 |
10647 | | |
10648 | | Position of copy of copy of copy of A_fixed_model_2_A.pdb (#61) relative to |
10649 | | W20_J160_run_ct6_body002.mrc (#33.2) coordinates: |
10650 | | Matrix rotation and translation |
10651 | | -0.32106500 -0.91005872 -0.26212669 270.65425094 |
10652 | | -0.60680985 -0.01482211 0.79470882 127.10234227 |
10653 | | -0.72711696 0.41421424 -0.54747373 232.51552841 |
10654 | | Axis -0.56535652 0.69090415 0.45058125 |
10655 | | Axis point 214.25644692 0.00000000 38.06080511 |
10656 | | Rotation angle (degrees) 160.33570313 |
10657 | | Shift along axis 39.56652768 |
10658 | | |
10659 | | |
10660 | | > hide #!33 models |
10661 | | |
10662 | | > show #!33 models |
10663 | | |
10664 | | > hide #!33 models |
10665 | | |
10666 | | Drag select of 10 residues |
10667 | | |
10668 | | > select up |
10669 | | |
10670 | | 388 atoms, 400 bonds, 50 residues, 1 model selected |
10671 | | |
10672 | | > delete sel |
10673 | | |
10674 | | Drag select of 88 residues |
10675 | | |
10676 | | > select clear |
10677 | | |
10678 | | Drag select of 57 residues |
10679 | | |
10680 | | > select clear |
10681 | | |
10682 | | Drag select of 78 residues |
10683 | | |
10684 | | > select up |
10685 | | |
10686 | | 719 atoms, 728 bonds, 89 residues, 1 model selected |
10687 | | |
10688 | | > delete sel |
10689 | | |
10690 | | Drag select of 3 residues |
10691 | | |
10692 | | > select up |
10693 | | |
10694 | | 125 atoms, 126 bonds, 16 residues, 1 model selected |
10695 | | |
10696 | | > delete sel |
10697 | | |
10698 | | > select clear |
10699 | | |
10700 | | > select #61/A:670 |
10701 | | |
10702 | | 8 atoms, 7 bonds, 1 residue, 1 model selected |
10703 | | |
10704 | | > select clear |
10705 | | |
10706 | | Drag select of 2 residues |
10707 | | |
10708 | | > select clear |
10709 | | |
10710 | | Drag select of 28 residues |
10711 | | |
10712 | | > fitmap #62 inMap #33.1 |
10713 | | |
10714 | | Fit molecule copy of copy of A_fixed_model_2_A.pdb (#62) to map |
10715 | | W20_J160_run_ct6_body001.mrc (#33.1) using 4038 atoms |
10716 | | average map value = 0.007876, steps = 40 |
10717 | | shifted from previous position = 0.0144 |
10718 | | rotated from previous position = 0.0178 degrees |
10719 | | atoms outside contour = 3169, contour level = 0.014811 |
10720 | | |
10721 | | Position of copy of copy of A_fixed_model_2_A.pdb (#62) relative to |
10722 | | W20_J160_run_ct6_body001.mrc (#33.1) coordinates: |
10723 | | Matrix rotation and translation |
10724 | | -0.49227524 -0.83177636 -0.25654079 286.90681543 |
10725 | | -0.53253090 0.05466159 0.84464368 111.49096886 |
10726 | | -0.68853172 0.55241307 -0.46985516 217.06478146 |
10727 | | Axis -0.48599732 0.71842724 0.49766344 |
10728 | | Axis point 201.81610476 0.00000000 36.65534481 |
10729 | | Rotation angle (degrees) 162.50332601 |
10730 | | Shift along axis 48.68741004 |
10731 | | |
10732 | | |
10733 | | > delete sel |
10734 | | |
10735 | | > select clear |
10736 | | |
10737 | | > fitmap #61 inMap #33.2 |
10738 | | |
10739 | | Fit molecule copy of copy of copy of A_fixed_model_2_A.pdb (#61) to map |
10740 | | W20_J160_run_ct6_body002.mrc (#33.2) using 2593 atoms |
10741 | | average map value = 0.01536, steps = 44 |
10742 | | shifted from previous position = 0.0309 |
10743 | | rotated from previous position = 0.286 degrees |
10744 | | atoms outside contour = 1295, contour level = 0.014809 |
10745 | | |
10746 | | Position of copy of copy of copy of A_fixed_model_2_A.pdb (#61) relative to |
10747 | | W20_J160_run_ct6_body002.mrc (#33.2) coordinates: |
10748 | | Matrix rotation and translation |
10749 | | -0.31750805 -0.91177971 -0.26047341 270.11347052 |
10750 | | -0.60893731 -0.01451577 0.79308552 127.53984411 |
10751 | | -0.72690026 0.41042302 -0.55060781 232.93839308 |
10752 | | Axis -0.56686193 0.69094730 0.44861941 |
10753 | | Axis point 214.45195639 0.00000000 38.26217280 |
10754 | | Rotation angle (degrees) 160.27371795 |
10755 | | Shift along axis 39.50695274 |
10756 | | |
10757 | | |
10758 | | > show #!33 models |
10759 | | |
10760 | | > hide #!33 models |
10761 | | |
10762 | | > hide #!61 models |
10763 | | |
10764 | | > show #!61 models |
10765 | | |
10766 | | Drag select of 8 residues |
10767 | | |
10768 | | > select up |
10769 | | |
10770 | | 138 atoms, 140 bonds, 17 residues, 1 model selected |
10771 | | |
10772 | | > select clear |
10773 | | |
10774 | | Drag select of 19 residues |
10775 | | |
10776 | | > select up |
10777 | | |
10778 | | 196 atoms, 200 bonds, 25 residues, 1 model selected |
10779 | | |
10780 | | > select clear |
10781 | | |
10782 | | Drag select of 3 residues |
10783 | | |
10784 | | > select up |
10785 | | |
10786 | | 41 atoms, 42 bonds, 5 residues, 1 model selected |
10787 | | |
10788 | | > delete sel |
10789 | | |
10790 | | > select #61/A:637 |
10791 | | |
10792 | | 7 atoms, 6 bonds, 1 residue, 1 model selected |
10793 | | |
10794 | | > delete sel |
10795 | | |
10796 | | Drag select of 2 residues |
10797 | | |
10798 | | > delete sel |
10799 | | |
10800 | | Drag select of 9 residues |
10801 | | |
10802 | | > delete sel |
10803 | | |
10804 | | > select #61/A:625 |
10805 | | |
10806 | | 8 atoms, 7 bonds, 1 residue, 1 model selected |
10807 | | |
10808 | | > delete sel |
10809 | | |
10810 | | > select #61/A:624 |
10811 | | |
10812 | | 6 atoms, 5 bonds, 1 residue, 1 model selected |
10813 | | |
10814 | | > delete sel |
10815 | | |
10816 | | > select #61/A:623 |
10817 | | |
10818 | | 9 atoms, 8 bonds, 1 residue, 1 model selected |
10819 | | |
10820 | | > delete sel |
10821 | | |
10822 | | > select clear |
10823 | | |
10824 | | [Repeated 1 time(s)] |
10825 | | |
10826 | | > select #61/A:622 |
10827 | | |
10828 | | 4 atoms, 3 bonds, 1 residue, 1 model selected |
10829 | | |
10830 | | > delete sel |
10831 | | |
10832 | | > fitmap #61 inMap #33.2 |
10833 | | |
10834 | | Fit molecule copy of copy of copy of A_fixed_model_2_A.pdb (#61) to map |
10835 | | W20_J160_run_ct6_body002.mrc (#33.2) using 2426 atoms |
10836 | | average map value = 0.01573, steps = 64 |
10837 | | shifted from previous position = 0.0485 |
10838 | | rotated from previous position = 0.404 degrees |
10839 | | atoms outside contour = 1173, contour level = 0.014809 |
10840 | | |
10841 | | Position of copy of copy of copy of A_fixed_model_2_A.pdb (#61) relative to |
10842 | | W20_J160_run_ct6_body002.mrc (#33.2) coordinates: |
10843 | | Matrix rotation and translation |
10844 | | -0.31268611 -0.91441000 -0.25706370 269.24111705 |
10845 | | -0.61103650 -0.01356040 0.79148626 127.91459578 |
10846 | | -0.72722884 0.40456206 -0.55449774 233.52795858 |
10847 | | Axis -0.56875957 0.69111966 0.44594414 |
10848 | | Axis point 214.64229202 0.00000000 38.50676211 |
10849 | | Rotation angle (degrees) 160.11414397 |
10850 | | Shift along axis 39.41125523 |
10851 | | |
10852 | | |
10853 | | > show #!33 models |
10854 | | |
10855 | | > show #!62 models |
10856 | | |
10857 | | > hide #!62 models |
10858 | | |
10859 | | > show #!60 models |
10860 | | |
10861 | | > hide #!61 models |
10862 | | |
10863 | | > hide #!60 models |
10864 | | |
10865 | | > show #!62 models |
10866 | | |
10867 | | > hide #!33 models |
10868 | | |
10869 | | Drag select of 2 residues |
10870 | | |
10871 | | > select up |
10872 | | |
10873 | | 60 atoms, 60 bonds, 7 residues, 1 model selected |
10874 | | |
10875 | | > select up |
10876 | | |
10877 | | 4038 atoms, 4115 bonds, 507 residues, 1 model selected |
10878 | | |
10879 | | > select down |
10880 | | |
10881 | | 60 atoms, 60 bonds, 7 residues, 1 model selected |
10882 | | |
10883 | | > select clear |
10884 | | |
10885 | | Drag select of 245 residues |
10886 | | |
10887 | | > select up |
10888 | | |
10889 | | 2197 atoms, 2238 bonds, 276 residues, 1 model selected |
10890 | | |
10891 | | > delete sel |
10892 | | |
10893 | | Drag select of 6 residues, 2 pseudobonds |
10894 | | |
10895 | | > select clear |
10896 | | |
10897 | | Drag select of 4 residues |
10898 | | |
10899 | | > select up |
10900 | | |
10901 | | 1841 atoms, 1872 bonds, 231 residues, 1 model selected |
10902 | | |
10903 | | > select down |
10904 | | |
10905 | | 36 atoms, 4 residues, 1 model selected |
10906 | | |
10907 | | > delete sel |
10908 | | |
10909 | | Drag select of 6 residues |
10910 | | |
10911 | | > delete sel |
10912 | | |
10913 | | Drag select of 12 residues |
10914 | | |
10915 | | > select up |
10916 | | |
10917 | | 116 atoms, 117 bonds, 13 residues, 1 model selected |
10918 | | |
10919 | | > delete sel |
10920 | | |
10921 | | > select clear |
10922 | | |
10923 | | [Repeated 2 time(s)] |
10924 | | |
10925 | | > select #62/A:618 |
10926 | | |
10927 | | 5 atoms, 4 bonds, 1 residue, 1 model selected |
10928 | | |
10929 | | > delete sel |
10930 | | |
10931 | | > select clear |
10932 | | |
10933 | | > select #62/A:619 |
10934 | | |
10935 | | 9 atoms, 8 bonds, 1 residue, 1 model selected |
10936 | | |
10937 | | > delete sel |
10938 | | |
10939 | | > select clear |
10940 | | |
10941 | | > select #62/A:620 |
10942 | | |
10943 | | 8 atoms, 7 bonds, 1 residue, 1 model selected |
10944 | | |
10945 | | > delete sel |
10946 | | |
10947 | | > select #62/A:621 |
10948 | | |
10949 | | 7 atoms, 6 bonds, 1 residue, 1 model selected |
10950 | | |
10951 | | > delete sel |
10952 | | |
10953 | | > fitmap #62 inMap #33.1 |
10954 | | |
10955 | | Fit molecule copy of copy of A_fixed_model_2_A.pdb (#62) to map |
10956 | | W20_J160_run_ct6_body001.mrc (#33.1) using 1612 atoms |
10957 | | average map value = 0.01422, steps = 100 |
10958 | | shifted from previous position = 1.05 |
10959 | | rotated from previous position = 7.03 degrees |
10960 | | atoms outside contour = 871, contour level = 0.014811 |
10961 | | |
10962 | | Position of copy of copy of A_fixed_model_2_A.pdb (#62) relative to |
10963 | | W20_J160_run_ct6_body001.mrc (#33.1) coordinates: |
10964 | | Matrix rotation and translation |
10965 | | -0.56465803 -0.76120461 -0.31894961 292.13379963 |
10966 | | -0.55687251 0.06616556 0.82795841 113.21112709 |
10967 | | -0.60914228 0.64512763 -0.46125484 203.93497656 |
10968 | | Axis -0.45795004 0.72686749 0.51180603 |
10969 | | Axis point 193.99705310 0.00000000 40.16949466 |
10970 | | Rotation angle (degrees) 168.48533814 |
10971 | | Shift along axis 52.88195186 |
10972 | | |
10973 | | |
10974 | | > show #!33 models |
10975 | | |
10976 | | > show #!61 models |
10977 | | |
10978 | | > show #!60 models |
10979 | | |
10980 | | > save "/Users/becca/Desktop/Postdoc/COPI notebooks and |
10981 | | > resources/Processing/W20_MM_cyto_COPI/20250704_W20_COPI_cyto_alignments.cxs" |
10982 | | |
10983 | | ——— End of log from Fri Jul 4 09:53:55 2025 ——— |
10984 | | |
10985 | | opened ChimeraX session |
10986 | | |
10987 | | > hide #!33 models |
10988 | | |
10989 | | > show #!33 models |
10990 | | |
10991 | | > open |
10992 | | > /Users/becca/Desktop/Postdoc/Phenix/W20_MM_cyto_COPI/PredictAndBuildCryoEM_1/PredictAndBuild_1_overall_best_map.ccp4 |
10993 | | |
10994 | | Opened PredictAndBuild_1_overall_best_map.ccp4 as #63, grid size 138,147,182, |
10995 | | pixel 1.2, shown at level 0.422, step 1, values float32 |
10996 | | |
10997 | | > select add #63 |
10998 | | |
10999 | | 2 models selected |
11000 | | |
11001 | | > select subtract #63 |
11002 | | |
11003 | | Nothing selected |
11004 | | |
11005 | | > hide #!33 models |
11006 | | |
11007 | | > volume #63 level 0.2752 |
11008 | | |
11009 | | > toolshed show |
11010 | | |
11011 | | Downloading bundle ChimeraX_locscalesurfer-0.1.1-py3-none-any.whl |
11012 | | Downloading bundle ChimeraX_ISOLDE-1.9-cp311-cp311-macosx_10_13_universal2.whl |
11013 | | [Repeated 1 time(s)] |
11014 | | |
11015 | | > select add #63 |
11016 | | |
11017 | | 2 models selected |
11018 | | |
11019 | | > show #!1 models |
11020 | | |
11021 | | > ui mousemode right "rotate selected models" |
11022 | | |
11023 | | > view matrix models |
11024 | | > #63,-0.58537,-0.79697,-0.1489,345.68,-0.68073,0.38338,0.6242,89.496,-0.44039,0.46675,-0.76694,243.81 |
11025 | | |
11026 | | > fitmap #63 inMap #33.2 |
11027 | | |
11028 | | Fit map PredictAndBuild_1_overall_best_map.ccp4 in map |
11029 | | W20_J160_run_ct6_body002.mrc using 134378 points |
11030 | | correlation = 0.4, correlation about mean = 0.0294, overlap = 163.4 |
11031 | | steps = 156, shift = 19.7, angle = 10.1 degrees |
11032 | | |
11033 | | Position of PredictAndBuild_1_overall_best_map.ccp4 (#63) relative to |
11034 | | W20_J160_run_ct6_body002.mrc (#33.2) coordinates: |
11035 | | Matrix rotation and translation |
11036 | | -0.49730946 -0.85158343 -0.16579799 356.32016493 |
11037 | | -0.56399341 0.17211906 0.80764255 80.81400003 |
11038 | | -0.65923801 0.49515725 -0.56588387 229.61138609 |
11039 | | Axis -0.47998711 0.75793917 0.44174720 |
11040 | | Axis point 229.49905252 0.00000000 52.36918700 |
11041 | | Rotation angle (degrees) 161.00326601 |
11042 | | Shift along axis -8.34680262 |
11043 | | |
11044 | | |
11045 | | > fitmap #63 inMap #2 |
11046 | | |
11047 | | Fit map PredictAndBuild_1_overall_best_map.ccp4 in map |
11048 | | W20_J3623_consensus_structure.mrc using 134378 points |
11049 | | correlation = 0.9819, correlation about mean = 0.7973, overlap = 2.007e+04 |
11050 | | steps = 112, shift = 10.7, angle = 7.31 degrees |
11051 | | |
11052 | | Position of PredictAndBuild_1_overall_best_map.ccp4 (#63) relative to |
11053 | | W20_J3623_consensus_structure.mrc (#2) coordinates: |
11054 | | Matrix rotation and translation |
11055 | | -0.34010494 -0.91904138 -0.19922745 353.84532994 |
11056 | | -0.77653019 0.15497541 0.61072375 146.03986464 |
11057 | | -0.53040504 0.36241629 -0.76637128 277.65026005 |
11058 | | Axis -0.56720681 0.75650641 0.32553722 |
11059 | | Axis point 251.99725576 0.00000000 81.48732765 |
11060 | | Rotation angle (degrees) 167.35637881 |
11061 | | Shift along axis 0.16210555 |
11062 | | |
11063 | | |
11064 | | > select subtract #63 |
11065 | | |
11066 | | Nothing selected |
11067 | | |
11068 | | > hide #!1 models |
11069 | | |
11070 | | > show #!11 models |
11071 | | |
11072 | | > hide #!11 models |
11073 | | |
11074 | | > show #!11 models |
11075 | | |
11076 | | > hide #!63 models |
11077 | | |
11078 | | > show #!63 models |
11079 | | |
11080 | | > hide #!11 models |
11081 | | |
11082 | | > show #!8 models |
11083 | | |
11084 | | > hide #!8 models |
11085 | | |
11086 | | > show #!7 models |
11087 | | |
11088 | | > hide #!63 models |
11089 | | |
11090 | | > show #!63 models |
11091 | | |
11092 | | > hide #!7 models |
11093 | | |
11094 | | > show #!7 models |
11095 | | |
11096 | | > hide #!7 models |
11097 | | |
11098 | | > show #!7 models |
11099 | | |
11100 | | > close #63 |
11101 | | |
11102 | | > open |
11103 | | > /Users/becca/Desktop/Postdoc/Phenix/W20_MM_cyto_COPI/PredictAndBuildCryoEM_1/PredictAndBuild_1_overall_best_superposed_predicted_models.pdb |
11104 | | |
11105 | | Chain information for |
11106 | | PredictAndBuild_1_overall_best_superposed_predicted_models.pdb #63 |
11107 | | --- |
11108 | | Chain | Description |
11109 | | A | No description available |
11110 | | B | No description available |
11111 | | F | No description available |
11112 | | |
11113 | | Computing secondary structure |
11114 | | |
11115 | | > open |
11116 | | > /Users/becca/Desktop/Postdoc/Phenix/W20_MM_cyto_COPI/PredictAndBuildCryoEM_1/PredictAndBuild_1_overall_best.pdb |
11117 | | |
11118 | | Chain information for PredictAndBuild_1_overall_best.pdb #64 |
11119 | | --- |
11120 | | Chain | Description |
11121 | | A | No description available |
11122 | | B | No description available |
11123 | | C | No description available |
11124 | | D | No description available |
11125 | | F | No description available |
11126 | | |
11127 | | |
11128 | | > open |
11129 | | > /Users/becca/Desktop/Postdoc/Phenix/W20_MM_cyto_COPI/PredictAndBuildCryoEM_1/PredictAndBuild_1_superposed_predicted_untrimmed_cycle_1.pdb |
11130 | | |
11131 | | Chain information for |
11132 | | PredictAndBuild_1_superposed_predicted_untrimmed_cycle_1.pdb #65 |
11133 | | --- |
11134 | | Chain | Description |
11135 | | A | No description available |
11136 | | B | No description available |
11137 | | F | No description available |
11138 | | |
11139 | | Computing secondary structure |
11140 | | |
11141 | | > select add #63 |
11142 | | |
11143 | | 13443 atoms, 13688 bonds, 16 pseudobonds, 1678 residues, 2 models selected |
11144 | | |
11145 | | > select add #64 |
11146 | | |
11147 | | 34205 atoms, 34848 bonds, 30 pseudobonds, 4274 residues, 4 models selected |
11148 | | |
11149 | | > select add #65 |
11150 | | |
11151 | | 52865 atoms, 53866 bonds, 30 pseudobonds, 6628 residues, 5 models selected |
11152 | | |
11153 | | > hide sel atoms |
11154 | | |
11155 | | > show sel cartoons |
11156 | | |
11157 | | Computing secondary structure |
11158 | | |
11159 | | > select subtract #65 |
11160 | | |
11161 | | 34205 atoms, 34848 bonds, 30 pseudobonds, 4274 residues, 4 models selected |
11162 | | |
11163 | | > select subtract #64 |
11164 | | |
11165 | | 13443 atoms, 13688 bonds, 16 pseudobonds, 1678 residues, 2 models selected |
11166 | | |
11167 | | > select subtract #63 |
11168 | | |
11169 | | Nothing selected |
11170 | | |
11171 | | > select add #63 |
11172 | | |
11173 | | 13443 atoms, 13688 bonds, 16 pseudobonds, 1678 residues, 2 models selected |
11174 | | |
11175 | | > select add #64 |
11176 | | |
11177 | | 34205 atoms, 34848 bonds, 30 pseudobonds, 4274 residues, 4 models selected |
11178 | | |
11179 | | > select add #65 |
11180 | | |
11181 | | 52865 atoms, 53866 bonds, 30 pseudobonds, 6628 residues, 5 models selected |
11182 | | |
11183 | | > view matrix models |
11184 | | > #63,-0.079462,-0.96928,-0.23276,330.08,-0.99279,0.055943,0.10597,250.15,-0.089693,0.2395,-0.96674,313.02,#64,-0.079462,-0.96928,-0.23276,330.08,-0.99279,0.055943,0.10597,250.15,-0.089693,0.2395,-0.96674,313.02,#65,-0.079462,-0.96928,-0.23276,330.08,-0.99279,0.055943,0.10597,250.15,-0.089693,0.2395,-0.96674,313.02 |
11185 | | |
11186 | | > ui mousemode right "translate selected models" |
11187 | | |
11188 | | > view matrix models |
11189 | | > #63,-0.079462,-0.96928,-0.23276,336.58,-0.99279,0.055943,0.10597,275.32,-0.089693,0.2395,-0.96674,259.8,#64,-0.079462,-0.96928,-0.23276,336.58,-0.99279,0.055943,0.10597,275.32,-0.089693,0.2395,-0.96674,259.8,#65,-0.079462,-0.96928,-0.23276,336.58,-0.99279,0.055943,0.10597,275.32,-0.089693,0.2395,-0.96674,259.8 |
11190 | | |
11191 | | > view matrix models |
11192 | | > #63,-0.079462,-0.96928,-0.23276,335,-0.99279,0.055943,0.10597,254.69,-0.089693,0.2395,-0.96674,251.24,#64,-0.079462,-0.96928,-0.23276,335,-0.99279,0.055943,0.10597,254.69,-0.089693,0.2395,-0.96674,251.24,#65,-0.079462,-0.96928,-0.23276,335,-0.99279,0.055943,0.10597,254.69,-0.089693,0.2395,-0.96674,251.24 |
11193 | | |
11194 | | > ui mousemode right "rotate selected models" |
11195 | | |
11196 | | > view matrix models |
11197 | | > #63,-0.095152,-0.88164,0.46222,203.93,-0.95238,0.21575,0.21547,206.02,-0.28969,-0.41971,-0.86019,359.38,#64,-0.095152,-0.88164,0.46222,203.93,-0.95238,0.21575,0.21547,206.02,-0.28969,-0.41971,-0.86019,359.38,#65,-0.095152,-0.88164,0.46222,203.93,-0.95238,0.21575,0.21547,206.02,-0.28969,-0.41971,-0.86019,359.38 |
11198 | | |
11199 | | > ui mousemode right "translate selected models" |
11200 | | |
11201 | | > view matrix models |
11202 | | > #63,-0.095152,-0.88164,0.46222,227.08,-0.95238,0.21575,0.21547,215.24,-0.28969,-0.41971,-0.86019,381.48,#64,-0.095152,-0.88164,0.46222,227.08,-0.95238,0.21575,0.21547,215.24,-0.28969,-0.41971,-0.86019,381.48,#65,-0.095152,-0.88164,0.46222,227.08,-0.95238,0.21575,0.21547,215.24,-0.28969,-0.41971,-0.86019,381.48 |
11203 | | |
11204 | | > hide #!7 models |
11205 | | |
11206 | | > show #!33 models |
11207 | | |
11208 | | > select subtract #65 |
11209 | | |
11210 | | 34205 atoms, 34848 bonds, 30 pseudobonds, 4274 residues, 4 models selected |
11211 | | |
11212 | | > select subtract #64 |
11213 | | |
11214 | | 13443 atoms, 13688 bonds, 16 pseudobonds, 1678 residues, 2 models selected |
11215 | | |
11216 | | > select subtract #63 |
11217 | | |
11218 | | Nothing selected |
11219 | | |
11220 | | > hide #65 models |
11221 | | |
11222 | | > hide #!64 models |
11223 | | |
11224 | | > fitmap #63 inMap #2 |
11225 | | |
11226 | | Fit molecule PredictAndBuild_1_overall_best_superposed_predicted_models.pdb |
11227 | | (#63) to map W20_J3623_consensus_structure.mrc (#2) using 13443 atoms |
11228 | | average map value = 0.3311, steps = 268 |
11229 | | shifted from previous position = 24.1 |
11230 | | rotated from previous position = 48.9 degrees |
11231 | | atoms outside contour = 4479, contour level = 0.25746 |
11232 | | |
11233 | | Position of PredictAndBuild_1_overall_best_superposed_predicted_models.pdb |
11234 | | (#63) relative to W20_J3623_consensus_structure.mrc (#2) coordinates: |
11235 | | Matrix rotation and translation |
11236 | | -0.33968216 -0.91923759 -0.19904344 353.65205126 |
11237 | | -0.77596080 0.15430364 0.61161690 145.90271071 |
11238 | | -0.53150812 0.36220526 -0.76570653 277.79017130 |
11239 | | Axis -0.56733253 0.75625188 0.32590934 |
11240 | | Axis point 251.98315191 0.00000000 81.43051773 |
11241 | | Rotation angle (degrees) 167.30207874 |
11242 | | Shift along axis 0.23529698 |
11243 | | |
11244 | | |
11245 | | > hide #!61 models |
11246 | | |
11247 | | > hide #!62 models |
11248 | | |
11249 | | > hide #!60 models |
11250 | | |
11251 | | > hide #!63 models |
11252 | | |
11253 | | > show #!63 models |
11254 | | |
11255 | | > hide #!63 models |
11256 | | |
11257 | | > show #!64 models |
11258 | | |
11259 | | > fitmap #64 inMap #2 |
11260 | | |
11261 | | Fit molecule PredictAndBuild_1_overall_best.pdb (#64) to map |
11262 | | W20_J3623_consensus_structure.mrc (#2) using 20762 atoms |
11263 | | average map value = 0.2661, steps = 552 |
11264 | | shifted from previous position = 15.4 |
11265 | | rotated from previous position = 48.8 degrees |
11266 | | atoms outside contour = 10565, contour level = 0.25746 |
11267 | | |
11268 | | Position of PredictAndBuild_1_overall_best.pdb (#64) relative to |
11269 | | W20_J3623_consensus_structure.mrc (#2) coordinates: |
11270 | | Matrix rotation and translation |
11271 | | -0.33968228 -0.91946019 -0.19801243 353.57410808 |
11272 | | -0.77672233 0.15550766 0.61034400 145.97075470 |
11273 | | -0.53039456 0.36112372 -0.76698845 277.98078879 |
11274 | | Axis -0.56734428 0.75666030 0.32493947 |
11275 | | Axis point 251.92912923 0.00000000 81.66482425 |
11276 | | Rotation angle (degrees) 167.31225097 |
11277 | | Shift along axis 0.17895697 |
11278 | | |
11279 | | |
11280 | | > hide #!64 models |
11281 | | |
11282 | | > show #65 models |
11283 | | |
11284 | | > fitmap #65 inMap #2 |
11285 | | |
11286 | | Fit molecule PredictAndBuild_1_superposed_predicted_untrimmed_cycle_1.pdb |
11287 | | (#65) to map W20_J3623_consensus_structure.mrc (#2) using 18660 atoms |
11288 | | average map value = 0.2436, steps = 340 |
11289 | | shifted from previous position = 22.1 |
11290 | | rotated from previous position = 48.8 degrees |
11291 | | atoms outside contour = 9859, contour level = 0.25746 |
11292 | | |
11293 | | Position of PredictAndBuild_1_superposed_predicted_untrimmed_cycle_1.pdb (#65) |
11294 | | relative to W20_J3623_consensus_structure.mrc (#2) coordinates: |
11295 | | Matrix rotation and translation |
11296 | | -0.34475277 -0.91893726 -0.19157204 353.38290459 |
11297 | | -0.77178767 0.16132165 0.61507653 143.76727401 |
11298 | | -0.53431202 0.35990227 -0.76484053 278.30475415 |
11299 | | Axis -0.56463658 0.75839755 0.32560511 |
11300 | | Axis point 251.20855212 0.00000000 81.61803612 |
11301 | | Rotation angle (degrees) 166.94047161 |
11302 | | Shift along axis 0.11728580 |
11303 | | |
11304 | | |
11305 | | > hide #65 models |
11306 | | |
11307 | | Downloading bundle ChimeraX_ISOLDE-1.9-cp311-cp311-macosx_10_13_universal2.whl |
11308 | | |
11309 | | > save "/Users/becca/Desktop/Postdoc/COPI notebooks and |
11310 | | > resources/Processing/W20_MM_cyto_COPI/20250704_W20_COPI_cyto_alignments.cxs" |
11311 | | |
11312 | | ——— End of log from Fri Jul 4 10:54:24 2025 ——— |
11313 | | |
11314 | | opened ChimeraX session |
11315 | | |
11316 | | > show #!46 models |
11317 | | |
11318 | | > hide #!46 models |
11319 | | |
11320 | | > hide #!50 models |
11321 | | |
11322 | | > show #!50 models |
11323 | | |
11324 | | > show #!60 models |
11325 | | |
11326 | | > hide #!60 models |
11327 | | |
11328 | | > show #!60 models |
11329 | | |
11330 | | > show #!61 models |
11331 | | |
11332 | | > hide #!61 models |
11333 | | |
11334 | | > show #!61 models |
11335 | | |
11336 | | > show #!62 models |
11337 | | |
11338 | | > color #60 #61 #62 navy |
11339 | | |
11340 | | > hide #!62 models |
11341 | | |
11342 | | > hide #!61 models |
11343 | | |
11344 | | > hide #!60 models |
11345 | | |
11346 | | > show #!60 models |
11347 | | |
11348 | | > show #!61 models |
11349 | | |
11350 | | > show #!62 models |
11351 | | |
11352 | | > close #57 |
11353 | | |
11354 | | > close #58 |
11355 | | |
11356 | | > close #59 |
11357 | | |
11358 | | > show #51 models |
11359 | | |
11360 | | > hide #51 models |
11361 | | |
11362 | | > close #51 |
11363 | | |
11364 | | > show #52 models |
11365 | | |
11366 | | > hide #!62 models |
11367 | | |
11368 | | > hide #!61 models |
11369 | | |
11370 | | > hide #!60 models |
11371 | | |
11372 | | > hide #!33 models |
11373 | | |
11374 | | > show #35 models |
11375 | | |
11376 | | > hide #35 models |
11377 | | |
11378 | | > combine #52 |
11379 | | |
11380 | | > hide #52 models |
11381 | | |
11382 | | > ui mousemode right select |
11383 | | |
11384 | | > select clear |
11385 | | |
11386 | | > select #51/B:953 |
11387 | | |
11388 | | 8 atoms, 7 bonds, 1 residue, 1 model selected |
11389 | | |
11390 | | > select up |
11391 | | |
11392 | | 54 atoms, 53 bonds, 7 residues, 1 model selected |
11393 | | |
11394 | | > select up |
11395 | | |
11396 | | 7500 atoms, 7618 bonds, 953 residues, 1 model selected |
11397 | | |
11398 | | > select down |
11399 | | |
11400 | | 54 atoms, 53 bonds, 7 residues, 1 model selected |
11401 | | |
11402 | | > select clear |
11403 | | |
11404 | | Drag select of 76 residues |
11405 | | |
11406 | | > select up |
11407 | | |
11408 | | 756 atoms, 763 bonds, 99 residues, 1 model selected |
11409 | | |
11410 | | > select clear |
11411 | | |
11412 | | Drag select of 58 residues |
11413 | | |
11414 | | > select up |
11415 | | |
11416 | | 773 atoms, 785 bonds, 99 residues, 1 model selected |
11417 | | |
11418 | | > delete sel |
11419 | | |
11420 | | Drag select of 7 residues |
11421 | | |
11422 | | > delete sel |
11423 | | |
11424 | | > select clear |
11425 | | |
11426 | | Drag select of 10 residues, 2 pseudobonds |
11427 | | |
11428 | | > select up |
11429 | | |
11430 | | 110 atoms, 109 bonds, 2 pseudobonds, 15 residues, 2 models selected |
11431 | | |
11432 | | > delete sel |
11433 | | |
11434 | | Drag select of 62 residues |
11435 | | |
11436 | | > select up |
11437 | | |
11438 | | 883 atoms, 897 bonds, 115 residues, 1 model selected |
11439 | | |
11440 | | > delete sel |
11441 | | |
11442 | | Drag select of 24 residues, 1 pseudobonds |
11443 | | |
11444 | | > delete sel |
11445 | | |
11446 | | Drag select of 2 residues |
11447 | | |
11448 | | > delete sel |
11449 | | |
11450 | | > show #!33 models |
11451 | | |
11452 | | > hide #!33 models |
11453 | | |
11454 | | Drag select of 13 residues |
11455 | | |
11456 | | > select up |
11457 | | |
11458 | | 126 atoms, 126 bonds, 17 residues, 1 model selected |
11459 | | |
11460 | | > delete sel |
11461 | | |
11462 | | Drag select of 7 residues |
11463 | | |
11464 | | > select up |
11465 | | |
11466 | | 69 atoms, 69 bonds, 9 residues, 1 model selected |
11467 | | |
11468 | | > delete sel |
11469 | | |
11470 | | > show #!33 models |
11471 | | |
11472 | | > select #51/B:664 |
11473 | | |
11474 | | 7 atoms, 6 bonds, 1 residue, 1 model selected |
11475 | | |
11476 | | > select up |
11477 | | |
11478 | | 215 atoms, 217 bonds, 27 residues, 1 model selected |
11479 | | |
11480 | | > delete sel |
11481 | | |
11482 | | > select clear |
11483 | | |
11484 | | > select #51/B:521 |
11485 | | |
11486 | | 12 atoms, 12 bonds, 1 residue, 1 model selected |
11487 | | |
11488 | | > select up |
11489 | | |
11490 | | 334 atoms, 338 bonds, 44 residues, 1 model selected |
11491 | | |
11492 | | > delete sel |
11493 | | |
11494 | | > fitmap #51 inMap #33.1 |
11495 | | |
11496 | | Fit molecule copy of B_fixed_model_2_B.pdb (#51) to map |
11497 | | W20_J160_run_ct6_body001.mrc (#33.1) using 4745 atoms |
11498 | | average map value = 0.01558, steps = 60 |
11499 | | shifted from previous position = 0.386 |
11500 | | rotated from previous position = 0.444 degrees |
11501 | | atoms outside contour = 2213, contour level = 0.014811 |
11502 | | |
11503 | | Position of copy of B_fixed_model_2_B.pdb (#51) relative to |
11504 | | W20_J160_run_ct6_body001.mrc (#33.1) coordinates: |
11505 | | Matrix rotation and translation |
11506 | | -0.39489665 -0.88949565 -0.22990026 277.26110723 |
11507 | | -0.63858809 0.08584769 0.76474533 131.14250865 |
11508 | | -0.66050124 0.44880693 -0.60192230 226.00577301 |
11509 | | Axis -0.53542046 0.72973902 0.42521276 |
11510 | | Axis point 208.19952618 0.00000000 43.73317487 |
11511 | | Rotation angle (degrees) 162.84020444 |
11512 | | Shift along axis 43.34907536 |
11513 | | |
11514 | | |
11515 | | > hide #!51 models |
11516 | | |
11517 | | > show #52 models |
11518 | | |
11519 | | > hide #!50 models |
11520 | | |
11521 | | > show #!50 models |
11522 | | |
11523 | | > hide #!33 models |
11524 | | |
11525 | | Drag select of 161 residues |
11526 | | |
11527 | | > select up |
11528 | | |
11529 | | 1472 atoms, 1497 bonds, 183 residues, 1 model selected |
11530 | | |
11531 | | > delete sel |
11532 | | |
11533 | | Drag select of 68 residues, 3 pseudobonds |
11534 | | |
11535 | | > select clear |
11536 | | |
11537 | | Drag select of 90 residues, 3 pseudobonds |
11538 | | |
11539 | | > select up |
11540 | | |
11541 | | 934 atoms, 946 bonds, 3 pseudobonds, 118 residues, 2 models selected |
11542 | | |
11543 | | > delete sel |
11544 | | |
11545 | | Drag select of 208 residues |
11546 | | |
11547 | | > select up |
11548 | | |
11549 | | 2197 atoms, 2217 bonds, 276 residues, 1 model selected |
11550 | | |
11551 | | > delete sel |
11552 | | |
11553 | | Drag select of 109 residues, 2 pseudobonds |
11554 | | |
11555 | | > select clear |
11556 | | |
11557 | | Drag select of 98 residues, 2 pseudobonds |
11558 | | |
11559 | | > select clear |
11560 | | |
11561 | | > select #52/B:624 |
11562 | | |
11563 | | 6 atoms, 5 bonds, 1 residue, 1 model selected |
11564 | | Drag select of 109 residues, 2 pseudobonds |
11565 | | |
11566 | | > select up |
11567 | | |
11568 | | 854 atoms, 863 bonds, 2 pseudobonds, 110 residues, 2 models selected |
11569 | | |
11570 | | > select down |
11571 | | |
11572 | | 844 atoms, 2 pseudobonds, 109 residues, 2 models selected |
11573 | | |
11574 | | > select clear |
11575 | | |
11576 | | Drag select of 109 residues, 2 pseudobonds |
11577 | | |
11578 | | > select up |
11579 | | |
11580 | | 854 atoms, 863 bonds, 2 pseudobonds, 110 residues, 2 models selected |
11581 | | |
11582 | | > delete sel |
11583 | | |
11584 | | > show #!33 models |
11585 | | |
11586 | | > show #!37 models |
11587 | | |
11588 | | > hide #!37 models |
11589 | | |
11590 | | > show #!37 models |
11591 | | |
11592 | | > hide #!37 models |
11593 | | |
11594 | | > hide #!33 models |
11595 | | |
11596 | | Drag select of 3 residues |
11597 | | |
11598 | | > select up |
11599 | | |
11600 | | 69 atoms, 69 bonds, 9 residues, 1 model selected |
11601 | | |
11602 | | > delete sel |
11603 | | |
11604 | | Drag select of 1 residues |
11605 | | |
11606 | | > select clear |
11607 | | |
11608 | | [Repeated 1 time(s)]Drag select of 2 residues |
11609 | | |
11610 | | > select up |
11611 | | |
11612 | | 98 atoms, 98 bonds, 13 residues, 1 model selected |
11613 | | |
11614 | | > select down |
11615 | | |
11616 | | 12 atoms, 2 residues, 1 model selected |
11617 | | |
11618 | | > select up |
11619 | | |
11620 | | 98 atoms, 98 bonds, 13 residues, 1 model selected |
11621 | | |
11622 | | > delete sel |
11623 | | |
11624 | | > show #!33 models |
11625 | | |
11626 | | > fitmap #62 inMap #33.1 |
11627 | | |
11628 | | Fit molecule copy of copy of A_fixed_model_2_A.pdb (#62) to map |
11629 | | W20_J160_run_ct6_body001.mrc (#33.1) using 1612 atoms |
11630 | | average map value = 0.01422, steps = 44 |
11631 | | shifted from previous position = 0.0171 |
11632 | | rotated from previous position = 0.0202 degrees |
11633 | | atoms outside contour = 870, contour level = 0.014811 |
11634 | | |
11635 | | Position of copy of copy of A_fixed_model_2_A.pdb (#62) relative to |
11636 | | W20_J160_run_ct6_body001.mrc (#33.1) coordinates: |
11637 | | Matrix rotation and translation |
11638 | | -0.56449972 -0.76141548 -0.31872643 292.10636189 |
11639 | | -0.55678517 0.06618546 0.82801555 113.19196963 |
11640 | | -0.60936880 0.64487670 -0.46130652 203.97357809 |
11641 | | Axis -0.45800977 0.72686404 0.51175748 |
11642 | | Axis point 194.00483060 0.00000000 40.16620251 |
11643 | | Rotation angle (degrees) 168.46719367 |
11644 | | Shift along axis 52.87260780 |
11645 | | |
11646 | | |
11647 | | > fitmap #52 inMap #33.1 |
11648 | | |
11649 | | Fit molecule B_fixed_model_2_B.pdb (#52) to map W20_J160_run_ct6_body001.mrc |
11650 | | (#33.1) using 1876 atoms |
11651 | | average map value = 0.01465, steps = 68 |
11652 | | shifted from previous position = 1.95 |
11653 | | rotated from previous position = 6.84 degrees |
11654 | | atoms outside contour = 924, contour level = 0.014811 |
11655 | | |
11656 | | Position of B_fixed_model_2_B.pdb (#52) relative to |
11657 | | W20_J160_run_ct6_body001.mrc (#33.1) coordinates: |
11658 | | Matrix rotation and translation |
11659 | | -0.32428809 -0.92759140 -0.18550319 265.85498427 |
11660 | | -0.58447784 0.04228657 0.81030704 122.87471915 |
11661 | | -0.74378954 0.37119543 -0.55586965 237.11232737 |
11662 | | Axis -0.55667228 0.70775295 0.43497325 |
11663 | | Axis point 213.01982007 0.00000000 38.79142480 |
11664 | | Rotation angle (degrees) 156.77096429 |
11665 | | Shift along axis 42.10836361 |
11666 | | |
11667 | | |
11668 | | > show #!51 models |
11669 | | |
11670 | | > show #!60 models |
11671 | | |
11672 | | > show #!61 models |
11673 | | |
11674 | | > show #!62 models |
11675 | | |
11676 | | > hide #!62 models |
11677 | | |
11678 | | > hide #!61 models |
11679 | | |
11680 | | > hide #!60 models |
11681 | | |
11682 | | > color #52 betav3 |
11683 | | |
11684 | | > color #53 betav3 |
11685 | | |
11686 | | > color #52 betav3 |
11687 | | |
11688 | | > color #52 betav1 |
11689 | | |
11690 | | > color #51 betav1 |
11691 | | |
11692 | | > show #!62 models |
11693 | | |
11694 | | > show #!61 models |
11695 | | |
11696 | | > show #!60 models |
11697 | | |
11698 | | > hide #!52 models |
11699 | | |
11700 | | > hide #!51 models |
11701 | | |
11702 | | > show #!53 models |
11703 | | |
11704 | | > color #53 betapv3 |
11705 | | |
11706 | | > hide #!53 models |
11707 | | |
11708 | | > show #50.1 models |
11709 | | |
11710 | | > hide #50.1 models |
11711 | | |
11712 | | > show #!50.2 models |
11713 | | |
11714 | | > hide #!50.2 models |
11715 | | |
11716 | | > show #!50.2 models |
11717 | | |
11718 | | > hide #!50.2 models |
11719 | | |
11720 | | > show #!50.3 models |
11721 | | |
11722 | | > hide #!50.3 models |
11723 | | |
11724 | | > show #!50.4 models |
11725 | | |
11726 | | > hide #!50.4 models |
11727 | | |
11728 | | > show #!50.5 models |
11729 | | |
11730 | | > hide #!50.5 models |
11731 | | |
11732 | | > show #50.6 models |
11733 | | |
11734 | | > show #!50.7 models |
11735 | | |
11736 | | > show #36 models |
11737 | | |
11738 | | > hide #36 models |
11739 | | |
11740 | | > show #36 models |
11741 | | |
11742 | | > ui tool show Matchmaker |
11743 | | |
11744 | | > matchmaker #50.6#!50.7 to #36 |
11745 | | |
11746 | | Parameters |
11747 | | --- |
11748 | | Chain pairing | bb |
11749 | | Alignment algorithm | Needleman-Wunsch |
11750 | | Similarity matrix | BLOSUM-62 |
11751 | | SS fraction | 0.3 |
11752 | | Gap open (HH/SS/other) | 18/18/6 |
11753 | | Gap extend | 1 |
11754 | | SS matrix | | | H | S | O |
11755 | | ---|---|---|--- |
11756 | | H | 6 | -9 | -6 |
11757 | | S | | 6 | -6 |
11758 | | O | | | 4 |
11759 | | Iteration cutoff | 2 |
11760 | | |
11761 | | Matchmaker CopBprime_O55029.pdb, chain A (#36) with |
11762 | | W20_C1_initial_model_no_wat_lig.pdb A5, chain A5 (#50.6), sequence alignment |
11763 | | score = 1599.3 |
11764 | | RMSD between 300 pruned atom pairs is 0.001 angstroms; (across all 300 pairs: |
11765 | | 0.001) |
11766 | | |
11767 | | Matchmaker CopBprime_O55029.pdb, chain A (#36) with |
11768 | | W20_C1_initial_model_no_wat_lig.pdb A6, chain A6 (#50.7), sequence alignment |
11769 | | score = 2777.6 |
11770 | | RMSD between 535 pruned atom pairs is 0.000 angstroms; (across all 535 pairs: |
11771 | | 0.000) |
11772 | | |
11773 | | |
11774 | | > hide #!60 models |
11775 | | |
11776 | | > hide #!61 models |
11777 | | |
11778 | | > hide #!62 models |
11779 | | |
11780 | | > hide #!33 models |
11781 | | |
11782 | | > show #!33 models |
11783 | | |
11784 | | > show #!29 models |
11785 | | |
11786 | | > hide #!33 models |
11787 | | |
11788 | | > hide #!29 models |
11789 | | |
11790 | | > show #!29 models |
11791 | | |
11792 | | > hide #!29 models |
11793 | | |
11794 | | > show #!7 models |
11795 | | |
11796 | | > hide #!7 models |
11797 | | |
11798 | | > show #!14 models |
11799 | | |
11800 | | > hide #!14 models |
11801 | | |
11802 | | > show #!15 models |
11803 | | |
11804 | | > hide #!15 models |
11805 | | |
11806 | | > show #!13 models |
11807 | | |
11808 | | > hide #!13 models |
11809 | | |
11810 | | > show #!29 models |
11811 | | |
11812 | | > hide #!29 models |
11813 | | |
11814 | | > show #!30 models |
11815 | | |
11816 | | > hide #!30 models |
11817 | | |
11818 | | > show #!31 models |
11819 | | |
11820 | | > hide #!31 models |
11821 | | |
11822 | | > show #!29 models |
11823 | | |
11824 | | > color #29 silver models |
11825 | | |
11826 | | > color #29 #c0c0c0bb models |
11827 | | |
11828 | | > fitmap #36 inMap #29.1 |
11829 | | |
11830 | | Fit molecule CopBprime_O55029.pdb (#36) to map W20_J140_run_body001.mrc |
11831 | | (#29.1) using 7214 atoms |
11832 | | average map value = 0.004094, steps = 196 |
11833 | | shifted from previous position = 6.85 |
11834 | | rotated from previous position = 8.52 degrees |
11835 | | atoms outside contour = 6338, contour level = 0.01322 |
11836 | | |
11837 | | Position of CopBprime_O55029.pdb (#36) relative to W20_J140_run_body001.mrc |
11838 | | (#29.1) coordinates: |
11839 | | Matrix rotation and translation |
11840 | | 0.34093560 0.24287995 0.90816972 145.36424309 |
11841 | | 0.84442936 0.34545023 -0.40939370 186.54646641 |
11842 | | -0.41316096 0.90646206 -0.08731868 180.11258285 |
11843 | | Axis 0.67156017 0.67435435 0.30700677 |
11844 | | Axis point 37.00439488 0.00000000 72.89161043 |
11845 | | Rotation angle (degrees) 101.56423540 |
11846 | | Shift along axis 278.71503775 |
11847 | | |
11848 | | |
11849 | | > fitmap #50.7 inMap #29.1 |
11850 | | |
11851 | | Fit molecule W20_C1_initial_model_no_wat_lig.pdb A6 (#50.7) to map |
11852 | | W20_J140_run_body001.mrc (#29.1) using 4264 atoms |
11853 | | average map value = 0.006635, steps = 664 |
11854 | | shifted from previous position = 6.45 |
11855 | | rotated from previous position = 16.7 degrees |
11856 | | atoms outside contour = 3295, contour level = 0.01322 |
11857 | | |
11858 | | Position of W20_C1_initial_model_no_wat_lig.pdb A6 (#50.7) relative to |
11859 | | W20_J140_run_body001.mrc (#29.1) coordinates: |
11860 | | Matrix rotation and translation |
11861 | | -0.98916440 0.14663035 -0.00730224 336.07347063 |
11862 | | -0.09208236 -0.65838724 -0.74702549 537.00653368 |
11863 | | -0.11434431 -0.73825862 0.66475529 165.63484820 |
11864 | | Axis 0.03349195 0.40893115 -0.91195044 |
11865 | | Axis point 189.25291418 293.62591224 0.00000000 |
11866 | | Rotation angle (degrees) 172.47953493 |
11867 | | Shift along axis 79.80368535 |
11868 | | |
11869 | | |
11870 | | > fitmap #50.6 inMap #29.4 |
11871 | | |
11872 | | Fit molecule W20_C1_initial_model_no_wat_lig.pdb A5 (#50.6) to map |
11873 | | W20_J140_run_body004.mrc (#29.4) using 2406 atoms |
11874 | | average map value = 0.01284, steps = 124 |
11875 | | shifted from previous position = 5.65 |
11876 | | rotated from previous position = 34.4 degrees |
11877 | | atoms outside contour = 1273, contour level = 0.012962 |
11878 | | |
11879 | | Position of W20_C1_initial_model_no_wat_lig.pdb A5 (#50.6) relative to |
11880 | | W20_J140_run_body004.mrc (#29.4) coordinates: |
11881 | | Matrix rotation and translation |
11882 | | -0.88088930 -0.47324067 0.00879232 419.53745111 |
11883 | | 0.15156939 -0.29963122 -0.94193835 456.11372933 |
11884 | | 0.44839799 -0.82841077 0.33567073 130.22033922 |
11885 | | Axis 0.14698922 -0.56917708 0.80896948 |
11886 | | Axis point 147.85180587 327.64605342 0.00000000 |
11887 | | Rotation angle (degrees) 157.28319935 |
11888 | | Shift along axis -92.59771834 |
11889 | | |
11890 | | |
11891 | | > hide #36 models |
11892 | | |
11893 | | > show #36 models |
11894 | | |
11895 | | > hide #36 models |
11896 | | |
11897 | | > hide #50.6 models |
11898 | | |
11899 | | > show #50.6 models |
11900 | | |
11901 | | > hide #50.6 models |
11902 | | |
11903 | | > hide #!29 models |
11904 | | |
11905 | | > show #50.6 models |
11906 | | |
11907 | | > show #36 models |
11908 | | |
11909 | | > hide #!50.7 models |
11910 | | |
11911 | | > hide #36 models |
11912 | | |
11913 | | > show #36 models |
11914 | | |
11915 | | > show #!53 models |
11916 | | |
11917 | | > hide #!53 models |
11918 | | |
11919 | | > close #53 |
11920 | | |
11921 | | > show #!63 models |
11922 | | |
11923 | | > hide #!63 models |
11924 | | |
11925 | | > close #63 |
11926 | | |
11927 | | > close #64 |
11928 | | |
11929 | | > close #65 |
11930 | | |
11931 | | > show #!50.7 models |
11932 | | |
11933 | | > combine #36 |
11934 | | |
11935 | | [Repeated 1 time(s)] |
11936 | | |
11937 | | > combine #50.7 |
11938 | | |
11939 | | > hide #!58 models |
11940 | | |
11941 | | > hide #!50.7 models |
11942 | | |
11943 | | > hide #57 models |
11944 | | |
11945 | | > hide #53 models |
11946 | | |
11947 | | > hide #36 models |
11948 | | |
11949 | | > show #!50.7 models |
11950 | | |
11951 | | > show #!58 models |
11952 | | |
11953 | | > hide #!58 models |
11954 | | |
11955 | | > show #!58 models |
11956 | | |
11957 | | > hide #!50.7 models |
11958 | | |
11959 | | > hide #!58 models |
11960 | | |
11961 | | > show #!58 models |
11962 | | |
11963 | | > hide #!58 models |
11964 | | |
11965 | | > combine #50.7 |
11966 | | |
11967 | | > hide #!59 models |
11968 | | |
11969 | | > show #!58 models |
11970 | | |
11971 | | > hide #50.6 models |
11972 | | |
11973 | | Drag select of 156 residues |
11974 | | |
11975 | | > select up |
11976 | | |
11977 | | 1543 atoms, 1574 bonds, 191 residues, 1 model selected |
11978 | | |
11979 | | > delete sel |
11980 | | |
11981 | | Drag select of 37 residues, 1 pseudobonds |
11982 | | |
11983 | | > select up |
11984 | | |
11985 | | 516 atoms, 519 bonds, 1 pseudobond, 65 residues, 2 models selected |
11986 | | |
11987 | | > delete sel |
11988 | | |
11989 | | Drag select of 7 residues, 1 pseudobonds |
11990 | | |
11991 | | > select clear |
11992 | | |
11993 | | Drag select of 6 residues, 1 pseudobonds |
11994 | | |
11995 | | > select up |
11996 | | |
11997 | | 54 atoms, 51 bonds, 1 pseudobond, 9 residues, 2 models selected |
11998 | | |
11999 | | > delete sel |
12000 | | |
12001 | | Drag select of 7 residues, 2 pseudobonds |
12002 | | |
12003 | | > select up |
12004 | | |
12005 | | 93 atoms, 93 bonds, 2 pseudobonds, 12 residues, 2 models selected |
12006 | | |
12007 | | > delete sel |
12008 | | |
12009 | | Drag select of 4 residues |
12010 | | |
12011 | | > delete sel |
12012 | | |
12013 | | > show #!29 models |
12014 | | |
12015 | | > fitmap #58 inMap #29.1 |
12016 | | |
12017 | | Fit molecule copy of W20_C1_initial_model_no_wat_lig.pdb A6 (#58) to map |
12018 | | W20_J140_run_body001.mrc (#29.1) using 2026 atoms |
12019 | | average map value = 0.01347, steps = 40 |
12020 | | shifted from previous position = 0.0954 |
12021 | | rotated from previous position = 0.431 degrees |
12022 | | atoms outside contour = 1055, contour level = 0.01322 |
12023 | | |
12024 | | Position of copy of W20_C1_initial_model_no_wat_lig.pdb A6 (#58) relative to |
12025 | | W20_J140_run_body001.mrc (#29.1) coordinates: |
12026 | | Matrix rotation and translation |
12027 | | -0.98886569 0.14811277 -0.01439634 337.90273979 |
12028 | | -0.08759046 -0.65752799 -0.74832136 536.28441247 |
12029 | | -0.12030195 -0.73872833 0.66318013 167.31953501 |
12030 | | Axis 0.03709874 0.40956482 -0.91152638 |
12031 | | Axis point 190.29117406 293.80476850 0.00000000 |
12032 | | Rotation angle (degrees) 172.57141027 |
12033 | | Shift along axis 79.66282889 |
12034 | | |
12035 | | |
12036 | | > show #!60 models |
12037 | | |
12038 | | > show #!61 models |
12039 | | |
12040 | | > show #!62 models |
12041 | | |
12042 | | > hide #!62 models |
12043 | | |
12044 | | > hide #!61 models |
12045 | | |
12046 | | > hide #!60 models |
12047 | | |
12048 | | > hide #!58 models |
12049 | | |
12050 | | > hide #!29 models |
12051 | | |
12052 | | > show #!59 models |
12053 | | |
12054 | | Drag select of 143 residues, 1 pseudobonds |
12055 | | |
12056 | | > select up |
12057 | | |
12058 | | 1146 atoms, 1169 bonds, 1 pseudobond, 145 residues, 2 models selected |
12059 | | |
12060 | | > delete sel |
12061 | | |
12062 | | > hide #!59 models |
12063 | | |
12064 | | > show #!58 models |
12065 | | |
12066 | | > hide #!58 models |
12067 | | |
12068 | | > show #!59 models |
12069 | | |
12070 | | Drag select of 51 residues |
12071 | | |
12072 | | > select up |
12073 | | |
12074 | | 444 atoms, 452 bonds, 55 residues, 1 model selected |
12075 | | |
12076 | | > delete sel |
12077 | | |
12078 | | Drag select of 27 residues |
12079 | | |
12080 | | > delete sel |
12081 | | |
12082 | | Drag select of 17 residues |
12083 | | |
12084 | | > select up |
12085 | | |
12086 | | 199 atoms, 201 bonds, 25 residues, 1 model selected |
12087 | | |
12088 | | > delete sel |
12089 | | |
12090 | | > select #59/A6:588 |
12091 | | |
12092 | | 8 atoms, 7 bonds, 1 residue, 1 model selected |
12093 | | |
12094 | | > select #59/A6:588 |
12095 | | |
12096 | | 8 atoms, 7 bonds, 1 residue, 1 model selected |
12097 | | |
12098 | | > show #!58 models |
12099 | | |
12100 | | > show #!29 models |
12101 | | |
12102 | | > hide #!29 models |
12103 | | |
12104 | | > hide #!58 models |
12105 | | |
12106 | | > delete sel |
12107 | | |
12108 | | > select #59/A6:587 |
12109 | | |
12110 | | 8 atoms, 7 bonds, 1 residue, 1 model selected |
12111 | | |
12112 | | > delete sel |
12113 | | |
12114 | | > show #!58 models |
12115 | | |
12116 | | > fitmap #59 inMap #29.4 |
12117 | | |
12118 | | Fit molecule copy of W20_C1_initial_model_no_wat_lig.pdb A6 (#59) to map |
12119 | | W20_J140_run_body004.mrc (#29.4) using 2238 atoms |
12120 | | average map value = 0.01488, steps = 128 |
12121 | | shifted from previous position = 9.04 |
12122 | | rotated from previous position = 24.7 degrees |
12123 | | atoms outside contour = 1046, contour level = 0.012962 |
12124 | | |
12125 | | Position of copy of W20_C1_initial_model_no_wat_lig.pdb A6 (#59) relative to |
12126 | | W20_J140_run_body004.mrc (#29.4) coordinates: |
12127 | | Matrix rotation and translation |
12128 | | -0.85413410 0.51616797 -0.06344729 245.49771496 |
12129 | | -0.20546679 -0.44701353 -0.87061031 558.08387697 |
12130 | | -0.47774295 -0.73058164 0.48786487 300.68201418 |
12131 | | Axis 0.16594922 0.49098549 -0.85521582 |
12132 | | Axis point 220.46254144 334.83017754 0.00000000 |
12133 | | Rotation angle (degrees) 155.04526429 |
12134 | | Shift along axis 57.60322791 |
12135 | | |
12136 | | |
12137 | | > show #!29 models |
12138 | | |
12139 | | > show #50.6 models |
12140 | | |
12141 | | > hide #!59 models |
12142 | | |
12143 | | > hide #!58 models |
12144 | | |
12145 | | > show #53 models |
12146 | | |
12147 | | > select #29.2 |
12148 | | |
12149 | | 2 models selected |
12150 | | |
12151 | | > select #29.2 |
12152 | | |
12153 | | 2 models selected |
12154 | | |
12155 | | > select clear |
12156 | | |
12157 | | [Repeated 1 time(s)] |
12158 | | |
12159 | | > select #53/A:876 |
12160 | | |
12161 | | 8 atoms, 7 bonds, 1 residue, 1 model selected |
12162 | | |
12163 | | > select up |
12164 | | |
12165 | | 519 atoms, 525 bonds, 67 residues, 1 model selected |
12166 | | |
12167 | | > delete sel |
12168 | | |
12169 | | > show #!58 models |
12170 | | |
12171 | | > hide #!58 models |
12172 | | |
12173 | | > select clear |
12174 | | |
12175 | | > select #53/A:640 |
12176 | | |
12177 | | 8 atoms, 7 bonds, 1 residue, 1 model selected |
12178 | | |
12179 | | > hide #!29 models |
12180 | | |
12181 | | Drag select of 184 residues |
12182 | | |
12183 | | > select up |
12184 | | |
12185 | | 1682 atoms, 1717 bonds, 208 residues, 1 model selected |
12186 | | |
12187 | | > delete sel |
12188 | | |
12189 | | > hide #50.6 models |
12190 | | |
12191 | | Drag select of 419 residues, 4 pseudobonds |
12192 | | |
12193 | | > select up |
12194 | | |
12195 | | 3440 atoms, 3516 bonds, 4 pseudobonds, 432 residues, 2 models selected |
12196 | | |
12197 | | > delete sel |
12198 | | |
12199 | | > fitmap #53 inMap #29.1 |
12200 | | |
12201 | | Fit molecule copy of CopBprime_O55029.pdb (#53) to map |
12202 | | W20_J140_run_body001.mrc (#29.1) using 1573 atoms |
12203 | | average map value = 0.0152, steps = 92 |
12204 | | shifted from previous position = 4.75 |
12205 | | rotated from previous position = 20.8 degrees |
12206 | | atoms outside contour = 682, contour level = 0.01322 |
12207 | | |
12208 | | Position of copy of CopBprime_O55029.pdb (#53) relative to |
12209 | | W20_J140_run_body001.mrc (#29.1) coordinates: |
12210 | | Matrix rotation and translation |
12211 | | 0.22399917 0.35238232 0.90865344 142.70226714 |
12212 | | 0.67056970 0.62083889 -0.40607308 181.66322382 |
12213 | | -0.70722037 0.70027550 -0.09722951 169.81132058 |
12214 | | Axis 0.55763236 0.81444812 0.16037584 |
12215 | | Axis point 78.89411363 0.00000000 66.67498198 |
12216 | | Rotation angle (degrees) 97.24981271 |
12217 | | Shift along axis 254.76430584 |
12218 | | |
12219 | | |
12220 | | > show #!29 models |
12221 | | |
12222 | | > hide #!53 models |
12223 | | |
12224 | | > show #!53 models |
12225 | | |
12226 | | > show #!58 models |
12227 | | |
12228 | | > hide #!58 models |
12229 | | |
12230 | | > hide #!53 models |
12231 | | |
12232 | | > show #!53 models |
12233 | | |
12234 | | > hide #!53 models |
12235 | | |
12236 | | > show #!53 models |
12237 | | |
12238 | | > hide #!53 models |
12239 | | |
12240 | | > show #!53 models |
12241 | | |
12242 | | > show #57 models |
12243 | | |
12244 | | > hide #57 models |
12245 | | |
12246 | | > hide #!29 models |
12247 | | |
12248 | | > hide #!53 models |
12249 | | |
12250 | | > show #57 models |
12251 | | |
12252 | | Drag select of 6 residues |
12253 | | |
12254 | | > select up |
12255 | | |
12256 | | 519 atoms, 525 bonds, 67 residues, 1 model selected |
12257 | | |
12258 | | > delete sel |
12259 | | |
12260 | | Drag select of 159 residues |
12261 | | |
12262 | | > select up |
12263 | | |
12264 | | 1356 atoms, 1383 bonds, 171 residues, 1 model selected |
12265 | | |
12266 | | > delete sel |
12267 | | |
12268 | | Drag select of 13 residues, 1 pseudobonds |
12269 | | |
12270 | | > select up |
12271 | | |
12272 | | 109 atoms, 108 bonds, 1 pseudobond, 14 residues, 2 models selected |
12273 | | |
12274 | | > delete sel |
12275 | | |
12276 | | Drag select of 5 residues |
12277 | | |
12278 | | > select clear |
12279 | | |
12280 | | Drag select of 8 residues |
12281 | | |
12282 | | > select up |
12283 | | |
12284 | | 108 atoms, 110 bonds, 13 residues, 1 model selected |
12285 | | |
12286 | | > delete sel |
12287 | | |
12288 | | > fitmap #57 inMap #29.4 |
12289 | | |
12290 | | Fit molecule copy of CopBprime_O55029.pdb (#57) to map |
12291 | | W20_J140_run_body004.mrc (#29.4) using 5122 atoms |
12292 | | average map value = 0.01184, steps = 144 |
12293 | | shifted from previous position = 8.94 |
12294 | | rotated from previous position = 13.8 degrees |
12295 | | atoms outside contour = 3092, contour level = 0.012962 |
12296 | | |
12297 | | Position of copy of CopBprime_O55029.pdb (#57) relative to |
12298 | | W20_J140_run_body004.mrc (#29.4) coordinates: |
12299 | | Matrix rotation and translation |
12300 | | 0.19336342 0.06063554 0.97925171 138.80893393 |
12301 | | 0.87031534 0.45017771 -0.19972793 186.94884028 |
12302 | | -0.45294790 0.89087786 0.03427586 177.78973972 |
12303 | | Axis 0.55251909 0.72557622 0.41019729 |
12304 | | Axis point 33.82238390 0.00000000 47.06660425 |
12305 | | Rotation angle (degrees) 99.27025680 |
12306 | | Shift along axis 285.26908646 |
12307 | | |
12308 | | |
12309 | | > show #!29 models |
12310 | | |
12311 | | > ui tool show "Build Structure" |
12312 | | |
12313 | | > toolshed show |
12314 | | |
12315 | | > show #!53 models |
12316 | | |
12317 | | > hide #!53 models |
12318 | | |
12319 | | > fitmap #57 inMap #29.4 |
12320 | | |
12321 | | Fit molecule copy of CopBprime_O55029.pdb (#57) to map |
12322 | | W20_J140_run_body004.mrc (#29.4) using 5122 atoms |
12323 | | average map value = 0.01184, steps = 104 |
12324 | | shifted from previous position = 0.0162 |
12325 | | rotated from previous position = 0.0152 degrees |
12326 | | atoms outside contour = 3088, contour level = 0.012962 |
12327 | | |
12328 | | Position of copy of CopBprime_O55029.pdb (#57) relative to |
12329 | | W20_J140_run_body004.mrc (#29.4) coordinates: |
12330 | | Matrix rotation and translation |
12331 | | 0.19340630 0.06050533 0.97925130 138.80565926 |
12332 | | 0.87021654 0.45038133 -0.19969933 186.94266371 |
12333 | | -0.45311938 0.89078379 0.03445385 177.80765427 |
12334 | | Axis 0.55243756 0.72563743 0.41019882 |
12335 | | Axis point 33.85424725 0.00000000 47.07180428 |
12336 | | Rotation angle (degrees) 99.25793540 |
12337 | | Shift along axis 285.27054234 |
12338 | | |
12339 | | |
12340 | | > combine #57 |
12341 | | |
12342 | | > hide #!29 models |
12343 | | |
12344 | | > hide #!63 models |
12345 | | |
12346 | | Drag select of 2 residues |
12347 | | |
12348 | | > select up |
12349 | | |
12350 | | 50 atoms, 50 bonds, 6 residues, 1 model selected |
12351 | | Drag select of 293 residues |
12352 | | |
12353 | | > select up |
12354 | | |
12355 | | 2400 atoms, 2461 bonds, 299 residues, 1 model selected |
12356 | | |
12357 | | > delete sel |
12358 | | |
12359 | | > fitmap #57 inMap #29.4 |
12360 | | |
12361 | | Fit molecule copy of CopBprime_O55029.pdb (#57) to map |
12362 | | W20_J140_run_body004.mrc (#29.4) using 2722 atoms |
12363 | | average map value = 0.01488, steps = 60 |
12364 | | shifted from previous position = 0.588 |
12365 | | rotated from previous position = 4.72 degrees |
12366 | | atoms outside contour = 1280, contour level = 0.012962 |
12367 | | |
12368 | | Position of copy of CopBprime_O55029.pdb (#57) relative to |
12369 | | W20_J140_run_body004.mrc (#29.4) coordinates: |
12370 | | Matrix rotation and translation |
12371 | | 0.24483069 0.00098871 0.96956534 140.64125601 |
12372 | | 0.85587662 0.46963714 -0.21660139 186.15669395 |
12373 | | -0.45555806 0.88285898 0.11413537 178.37372881 |
12374 | | Axis 0.55176004 0.71519280 0.42902227 |
12375 | | Axis point 33.88188669 0.00000000 44.81013344 |
12376 | | Rotation angle (degrees) 94.91618649 |
12377 | | Shift along axis 287.26445575 |
12378 | | |
12379 | | |
12380 | | > show #!29 models |
12381 | | |
12382 | | > select add #57 |
12383 | | |
12384 | | 2722 atoms, 2779 bonds, 341 residues, 1 model selected |
12385 | | |
12386 | | > select clear |
12387 | | |
12388 | | > select add #57 |
12389 | | |
12390 | | 2722 atoms, 2779 bonds, 341 residues, 1 model selected |
12391 | | |
12392 | | > ui mousemode right "translate selected models" |
12393 | | |
12394 | | > view matrix models |
12395 | | > #57,0.50755,-0.10762,0.85488,130.21,0.68026,0.65898,-0.32092,190.13,-0.52881,0.74442,0.40768,177.92 |
12396 | | |
12397 | | > fitmap #57 inMap #29.4 |
12398 | | |
12399 | | Fit molecule copy of CopBprime_O55029.pdb (#57) to map |
12400 | | W20_J140_run_body004.mrc (#29.4) using 2722 atoms |
12401 | | average map value = 0.01488, steps = 64 |
12402 | | shifted from previous position = 2.72 |
12403 | | rotated from previous position = 0.042 degrees |
12404 | | atoms outside contour = 1279, contour level = 0.012962 |
12405 | | |
12406 | | Position of copy of CopBprime_O55029.pdb (#57) relative to |
12407 | | W20_J140_run_body004.mrc (#29.4) coordinates: |
12408 | | Matrix rotation and translation |
12409 | | 0.24476466 0.00100564 0.96958200 140.62777342 |
12410 | | 0.85622153 0.46899298 -0.21663398 186.17961813 |
12411 | | -0.45494501 0.88320132 0.11393185 178.36605751 |
12412 | | Axis 0.55197003 0.71492178 0.42920384 |
12413 | | Axis point 33.76576678 0.00000000 44.80708945 |
12414 | | Rotation angle (degrees) 94.94245961 |
12415 | | Shift along axis 287.28157686 |
12416 | | |
12417 | | |
12418 | | > select subtract #57 |
12419 | | |
12420 | | Nothing selected |
12421 | | |
12422 | | > hide #!57 models |
12423 | | |
12424 | | > show #!63 models |
12425 | | |
12426 | | > hide #!29 models |
12427 | | |
12428 | | > ui mousemode right select |
12429 | | |
12430 | | Drag select of 285 residues |
12431 | | |
12432 | | > select up |
12433 | | |
12434 | | 2450 atoms, 2494 bonds, 306 residues, 1 model selected |
12435 | | |
12436 | | > select up |
12437 | | |
12438 | | 5122 atoms, 5241 bonds, 640 residues, 1 model selected |
12439 | | |
12440 | | > select clear |
12441 | | |
12442 | | Drag select of 338 residues |
12443 | | |
12444 | | > select up |
12445 | | |
12446 | | 2722 atoms, 2779 bonds, 341 residues, 1 model selected |
12447 | | |
12448 | | > select clear |
12449 | | |
12450 | | Drag select of 321 residues |
12451 | | |
12452 | | > select up |
12453 | | |
12454 | | 2643 atoms, 2694 bonds, 331 residues, 1 model selected |
12455 | | |
12456 | | > select clear |
12457 | | |
12458 | | Drag select of 193 residues |
12459 | | |
12460 | | > select up |
12461 | | |
12462 | | 2186 atoms, 2221 bonds, 272 residues, 1 model selected |
12463 | | |
12464 | | > delete sel |
12465 | | |
12466 | | > show #!57 models |
12467 | | |
12468 | | > hide #!63 models |
12469 | | |
12470 | | > hide #!57 models |
12471 | | |
12472 | | > show #!63 models |
12473 | | |
12474 | | > select #63/A:10 |
12475 | | |
12476 | | 9 atoms, 8 bonds, 1 residue, 1 model selected |
12477 | | |
12478 | | > select #63/A:577 |
12479 | | |
12480 | | 8 atoms, 7 bonds, 1 residue, 1 model selected |
12481 | | |
12482 | | > select up |
12483 | | |
12484 | | 219 atoms, 213 bonds, 27 residues, 1 model selected |
12485 | | |
12486 | | > delete sel |
12487 | | |
12488 | | > select clear |
12489 | | |
12490 | | > select #63/A:430 |
12491 | | |
12492 | | 5 atoms, 4 bonds, 1 residue, 1 model selected |
12493 | | Drag select of 14 residues, 1 pseudobonds |
12494 | | |
12495 | | > select clear |
12496 | | |
12497 | | Drag select of 16 residues, 3 pseudobonds |
12498 | | |
12499 | | > select up |
12500 | | |
12501 | | 125 atoms, 125 bonds, 3 pseudobonds, 17 residues, 2 models selected |
12502 | | |
12503 | | > delete sel |
12504 | | |
12505 | | Drag select of 3 residues |
12506 | | |
12507 | | > delete sel |
12508 | | |
12509 | | Drag select of 3 residues |
12510 | | |
12511 | | > select up |
12512 | | |
12513 | | 42 atoms, 41 bonds, 6 residues, 1 model selected |
12514 | | |
12515 | | > delete sel |
12516 | | |
12517 | | > select clear |
12518 | | |
12519 | | > select #63/A:353 |
12520 | | |
12521 | | 8 atoms, 7 bonds, 1 residue, 1 model selected |
12522 | | |
12523 | | > select up |
12524 | | |
12525 | | 124 atoms, 124 bonds, 16 residues, 1 model selected |
12526 | | |
12527 | | > select clear |
12528 | | |
12529 | | [Repeated 1 time(s)]Drag select of 1 residues |
12530 | | |
12531 | | > select down |
12532 | | |
12533 | | 4 atoms, 1 residue, 1 model selected |
12534 | | |
12535 | | > select up |
12536 | | |
12537 | | 124 atoms, 124 bonds, 16 residues, 1 model selected |
12538 | | |
12539 | | > select clear |
12540 | | |
12541 | | > select #63/A:373 |
12542 | | |
12543 | | 4 atoms, 3 bonds, 1 residue, 1 model selected |
12544 | | |
12545 | | > delete sel |
12546 | | |
12547 | | > select clear |
12548 | | |
12549 | | > select #63/A:354 |
12550 | | |
12551 | | 12 atoms, 12 bonds, 1 residue, 1 model selected |
12552 | | |
12553 | | > delete sel |
12554 | | |
12555 | | > select clear |
12556 | | |
12557 | | > select #63/A:356 |
12558 | | |
12559 | | 9 atoms, 8 bonds, 1 residue, 1 model selected |
12560 | | |
12561 | | > \ |
12562 | | |
12563 | | Unknown command: \ |
12564 | | |
12565 | | > select clear |
12566 | | |
12567 | | > select #63/A:355 |
12568 | | |
12569 | | 7 atoms, 7 bonds, 1 residue, 1 model selected |
12570 | | |
12571 | | > \ |
12572 | | |
12573 | | Unknown command: \ |
12574 | | |
12575 | | > delete sel |
12576 | | |
12577 | | > select clear |
12578 | | |
12579 | | [Repeated 1 time(s)]Drag select of 3 residues, 2 pseudobonds |
12580 | | |
12581 | | > delete sel |
12582 | | |
12583 | | > select #63/A:320 |
12584 | | |
12585 | | 6 atoms, 5 bonds, 1 residue, 1 model selected |
12586 | | Drag select of 1 residues, 2 pseudobonds |
12587 | | |
12588 | | > select up |
12589 | | |
12590 | | 78 atoms, 76 bonds, 2 pseudobonds, 10 residues, 2 models selected |
12591 | | |
12592 | | > close #63 |
12593 | | |
12594 | | > combine #36 |
12595 | | |
12596 | | Drag select of 280 residues |
12597 | | |
12598 | | > select up |
12599 | | |
12600 | | 3003 atoms, 3050 bonds, 379 residues, 1 model selected |
12601 | | |
12602 | | > delete sel |
12603 | | |
12604 | | Drag select of 41 residues, 2 pseudobonds |
12605 | | |
12606 | | > delete sel |
12607 | | |
12608 | | Drag select of 122 residues, 3 pseudobonds |
12609 | | |
12610 | | > select up |
12611 | | |
12612 | | 1221 atoms, 1239 bonds, 3 pseudobonds, 152 residues, 2 models selected |
12613 | | |
12614 | | > delete sel |
12615 | | |
12616 | | Drag select of 9 residues, 2 pseudobonds |
12617 | | |
12618 | | > select up |
12619 | | |
12620 | | 91 atoms, 90 bonds, 2 pseudobonds, 12 residues, 2 models selected |
12621 | | |
12622 | | > delete sel |
12623 | | |
12624 | | > select #63/A:373 |
12625 | | |
12626 | | 4 atoms, 3 bonds, 1 residue, 1 model selected |
12627 | | |
12628 | | > delete sel |
12629 | | |
12630 | | > select #63/A:356 |
12631 | | |
12632 | | 9 atoms, 8 bonds, 1 residue, 1 model selected |
12633 | | |
12634 | | > delete sel |
12635 | | |
12636 | | > close #63 |
12637 | | |
12638 | | > show #!53 models |
12639 | | |
12640 | | > hide #!53 models |
12641 | | |
12642 | | > show #!57 models |
12643 | | |
12644 | | > hide #!57 models |
12645 | | |
12646 | | > show #50.1 models |
12647 | | |
12648 | | > hide #50.1 models |
12649 | | |
12650 | | > show #!50.2 models |
12651 | | |
12652 | | > hide #!50.2 models |
12653 | | |
12654 | | > combine #36 |
12655 | | |
12656 | | Drag select of 289 residues |
12657 | | |
12658 | | > select up |
12659 | | |
12660 | | 2306 atoms, 2348 bonds, 291 residues, 1 model selected |
12661 | | |
12662 | | > delete sel |
12663 | | |
12664 | | Drag select of 218 residues |
12665 | | |
12666 | | > select up |
12667 | | |
12668 | | 1888 atoms, 1925 bonds, 237 residues, 1 model selected |
12669 | | |
12670 | | > delete sel |
12671 | | |
12672 | | Drag select of 15 residues, 1 pseudobonds |
12673 | | |
12674 | | > select up |
12675 | | |
12676 | | 152 atoms, 153 bonds, 1 pseudobond, 18 residues, 2 models selected |
12677 | | |
12678 | | > delete sel |
12679 | | |
12680 | | Drag select of 4 residues |
12681 | | |
12682 | | > select up |
12683 | | |
12684 | | 42 atoms, 41 bonds, 5 residues, 1 model selected |
12685 | | |
12686 | | > delete sel |
12687 | | |
12688 | | Drag select of 29 residues, 2 pseudobonds |
12689 | | |
12690 | | > select up |
12691 | | |
12692 | | 302 atoms, 302 bonds, 2 pseudobonds, 39 residues, 2 models selected |
12693 | | |
12694 | | > delete sel |
12695 | | |
12696 | | > select clear |
12697 | | |
12698 | | [Repeated 1 time(s)] |
12699 | | |
12700 | | > select #63/A:305 |
12701 | | |
12702 | | 5 atoms, 4 bonds, 1 residue, 1 model selected |
12703 | | |
12704 | | > delete sel |
12705 | | |
12706 | | > select clear |
12707 | | |
12708 | | Drag select of 8 residues, 1 pseudobonds |
12709 | | |
12710 | | > select up |
12711 | | |
12712 | | 119 atoms, 119 bonds, 1 pseudobond, 15 residues, 2 models selected |
12713 | | |
12714 | | > show #!57 models |
12715 | | |
12716 | | > delete sel |
12717 | | |
12718 | | > fitmap #63 inMap #29.4 |
12719 | | |
12720 | | Fit molecule copy of CopBprime_O55029.pdb (#63) to map |
12721 | | W20_J140_run_body004.mrc (#29.4) using 2400 atoms |
12722 | | average map value = 0.01282, steps = 148 |
12723 | | shifted from previous position = 15.9 |
12724 | | rotated from previous position = 27.5 degrees |
12725 | | atoms outside contour = 1275, contour level = 0.012962 |
12726 | | |
12727 | | Position of copy of CopBprime_O55029.pdb (#63) relative to |
12728 | | W20_J140_run_body004.mrc (#29.4) coordinates: |
12729 | | Matrix rotation and translation |
12730 | | 0.53194142 0.55442248 0.64004222 123.33487031 |
12731 | | 0.70035644 0.13679328 -0.70056295 183.98600531 |
12732 | | -0.47596132 0.82091614 -0.31552735 169.31259792 |
12733 | | Axis 0.80394040 0.58968956 0.07711063 |
12734 | | Axis point 0.00000000 -20.96531712 102.68367487 |
12735 | | Rotation angle (degrees) 108.86844509 |
12736 | | Shift along axis 220.70431310 |
12737 | | |
12738 | | |
12739 | | > show #!29 models |
12740 | | |
12741 | | > show #!53 models |
12742 | | |
12743 | | > show #!58 models |
12744 | | |
12745 | | > hide #!58 models |
12746 | | |
12747 | | > show #!58 models |
12748 | | |
12749 | | > hide #!53 models |
12750 | | |
12751 | | > show #!53 models |
12752 | | |
12753 | | > hide #!57 models |
12754 | | |
12755 | | > show #!57 models |
12756 | | |
12757 | | > hide #!57 models |
12758 | | |
12759 | | > show #!59 models |
12760 | | |
12761 | | > hide #!53 models |
12762 | | |
12763 | | > hide #!63 models |
12764 | | |
12765 | | > show #!63 models |
12766 | | |
12767 | | > hide #!63 models |
12768 | | |
12769 | | > show #!57 models |
12770 | | |
12771 | | > hide #!57 models |
12772 | | |
12773 | | > show #!57 models |
12774 | | |
12775 | | > hide #!57 models |
12776 | | |
12777 | | > show #!53 models |
12778 | | |
12779 | | > hide #!53 models |
12780 | | |
12781 | | > show #!53 models |
12782 | | |
12783 | | > hide #!53 models |
12784 | | |
12785 | | > show #!63 models |
12786 | | |
12787 | | > hide #!63 models |
12788 | | |
12789 | | > show #50.6 models |
12790 | | |
12791 | | > show #!63 models |
12792 | | |
12793 | | > hide #!63 models |
12794 | | |
12795 | | > show #!53 models |
12796 | | |
12797 | | > hide #!53 models |
12798 | | |
12799 | | > show #!53 models |
12800 | | |
12801 | | > hide #!53 models |
12802 | | |
12803 | | > show #!57 models |
12804 | | |
12805 | | > hide #!57 models |
12806 | | |
12807 | | > show #!57 models |
12808 | | |
12809 | | > hide #!57 models |
12810 | | |
12811 | | > combine #57 |
12812 | | |
12813 | | > hide #!58 models |
12814 | | |
12815 | | > hide #!59 models |
12816 | | |
12817 | | > hide #50.6 models |
12818 | | |
12819 | | > show #!63 models |
12820 | | |
12821 | | > hide #!63 models |
12822 | | |
12823 | | > show #!63 models |
12824 | | |
12825 | | > hide #!63 models |
12826 | | |
12827 | | > show #!63 models |
12828 | | |
12829 | | > hide #!29 models |
12830 | | |
12831 | | > show #50.6 models |
12832 | | |
12833 | | > hide #50.6 models |
12834 | | |
12835 | | > hide #!63 models |
12836 | | |
12837 | | > show #!57 models |
12838 | | |
12839 | | > hide #!57 models |
12840 | | |
12841 | | > show #!57 models |
12842 | | |
12843 | | > hide #!57 models |
12844 | | |
12845 | | Drag select of 26 residues |
12846 | | |
12847 | | > select up |
12848 | | |
12849 | | 229 atoms, 232 bonds, 28 residues, 1 model selected |
12850 | | |
12851 | | > delete sel |
12852 | | |
12853 | | Drag select of 9 residues |
12854 | | |
12855 | | > select up |
12856 | | |
12857 | | 76 atoms, 77 bonds, 10 residues, 1 model selected |
12858 | | |
12859 | | > delete sel |
12860 | | |
12861 | | Drag select of 6 residues |
12862 | | |
12863 | | > select clear |
12864 | | |
12865 | | Drag select of 10 residues |
12866 | | |
12867 | | > select up |
12868 | | |
12869 | | 115 atoms, 115 bonds, 14 residues, 1 model selected |
12870 | | |
12871 | | > combine #57 |
12872 | | |
12873 | | > select add #64 |
12874 | | |
12875 | | 2417 atoms, 2468 bonds, 303 residues, 1 model selected |
12876 | | |
12877 | | > close #64 |
12878 | | |
12879 | | > hide #!65 models |
12880 | | |
12881 | | > show #!65 models |
12882 | | |
12883 | | > show #!63 models |
12884 | | |
12885 | | > hide #!63 models |
12886 | | |
12887 | | > show #!63 models |
12888 | | |
12889 | | > hide #!63 models |
12890 | | |
12891 | | > show #!57 models |
12892 | | |
12893 | | > hide #!57 models |
12894 | | |
12895 | | > show #!57 models |
12896 | | |
12897 | | > hide #!65 models |
12898 | | |
12899 | | Drag select of 31 residues |
12900 | | |
12901 | | > select up |
12902 | | |
12903 | | 305 atoms, 310 bonds, 38 residues, 1 model selected |
12904 | | |
12905 | | > delete sel |
12906 | | |
12907 | | Drag select of 13 residues |
12908 | | |
12909 | | > select up |
12910 | | |
12911 | | 115 atoms, 115 bonds, 14 residues, 1 model selected |
12912 | | |
12913 | | > delete sel |
12914 | | |
12915 | | > hide #!57 models |
12916 | | |
12917 | | > show #!53 models |
12918 | | |
12919 | | > show #!57 models |
12920 | | |
12921 | | > fitmap #57 inMap #29.4 |
12922 | | |
12923 | | Fit molecule copy of CopBprime_O55029.pdb (#57) to map |
12924 | | W20_J140_run_body004.mrc (#29.4) using 2302 atoms |
12925 | | average map value = 0.01478, steps = 84 |
12926 | | shifted from previous position = 0.608 |
12927 | | rotated from previous position = 2.11 degrees |
12928 | | atoms outside contour = 1083, contour level = 0.012962 |
12929 | | |
12930 | | Position of copy of CopBprime_O55029.pdb (#57) relative to |
12931 | | W20_J140_run_body004.mrc (#29.4) coordinates: |
12932 | | Matrix rotation and translation |
12933 | | 0.26430656 -0.02399441 0.96414019 140.91312010 |
12934 | | 0.84653335 0.48474763 -0.22000233 186.26213690 |
12935 | | -0.46208585 0.87432489 0.14843404 179.06445534 |
12936 | | Axis 0.54788377 0.71405160 0.43583676 |
12937 | | Axis point 34.54414646 0.00000000 44.00500794 |
12938 | | Rotation angle (degrees) 92.93803311 |
12939 | | Shift along axis 288.24766132 |
12940 | | |
12941 | | |
12942 | | > show #!63 models |
12943 | | |
12944 | | > show #!65 models |
12945 | | |
12946 | | > hide #!65 models |
12947 | | |
12948 | | > show #!65 models |
12949 | | |
12950 | | > hide #!65 models |
12951 | | |
12952 | | > show #!65 models |
12953 | | |
12954 | | > hide #!57 models |
12955 | | |
12956 | | > hide #!63 models |
12957 | | |
12958 | | Drag select of 243 residues |
12959 | | |
12960 | | > select up |
12961 | | |
12962 | | 2119 atoms, 2164 bonds, 266 residues, 1 model selected |
12963 | | |
12964 | | > delete sel |
12965 | | |
12966 | | > hide #!65 models |
12967 | | |
12968 | | > show #!57 models |
12969 | | |
12970 | | > show #!63 models |
12971 | | |
12972 | | > hide #!63 models |
12973 | | |
12974 | | > hide #!57 models |
12975 | | |
12976 | | > show #!65 models |
12977 | | |
12978 | | Drag select of 17 residues, 1 pseudobonds |
12979 | | |
12980 | | > select up |
12981 | | |
12982 | | 159 atoms, 159 bonds, 1 pseudobond, 19 residues, 2 models selected |
12983 | | Drag select of 22 residues, 2 pseudobonds |
12984 | | |
12985 | | > delete sel |
12986 | | |
12987 | | > show #!57 models |
12988 | | |
12989 | | > select #57/A:547 |
12990 | | |
12991 | | 7 atoms, 6 bonds, 1 residue, 1 model selected |
12992 | | |
12993 | | > select clear |
12994 | | |
12995 | | > show #!29 models |
12996 | | |
12997 | | > hide #!29 models |
12998 | | |
12999 | | > hide #!57 models |
13000 | | |
13001 | | > select #65/A:588 |
13002 | | |
13003 | | 8 atoms, 7 bonds, 1 residue, 1 model selected |
13004 | | |
13005 | | > delete sel |
13006 | | |
13007 | | > show #!57 models |
13008 | | |
13009 | | > fitmap #64 inMap #29.4 |
13010 | | |
13011 | | No atoms or maps for #64 |
13012 | | |
13013 | | > fitmap #65 inMap #29.4 |
13014 | | |
13015 | | Fit molecule copy of copy of CopBprime_O55029.pdb (#65) to map |
13016 | | W20_J140_run_body004.mrc (#29.4) using 420 atoms |
13017 | | average map value = 0.02083, steps = 56 |
13018 | | shifted from previous position = 2.51 |
13019 | | rotated from previous position = 13.6 degrees |
13020 | | atoms outside contour = 145, contour level = 0.012962 |
13021 | | |
13022 | | Position of copy of copy of CopBprime_O55029.pdb (#65) relative to |
13023 | | W20_J140_run_body004.mrc (#29.4) coordinates: |
13024 | | Matrix rotation and translation |
13025 | | 0.25505166 0.23428436 0.93811486 140.41746282 |
13026 | | 0.81508493 0.46984578 -0.33894174 184.56399264 |
13027 | | -0.52017805 0.85109094 -0.07112670 178.31682392 |
13028 | | Axis 0.60413780 0.74032411 0.29485206 |
13029 | | Axis point 50.19505778 0.00000000 66.66205089 |
13030 | | Rotation angle (degrees) 99.96896003 |
13031 | | Shift along axis 274.04575231 |
13032 | | |
13033 | | |
13034 | | > show #!29 models |
13035 | | |
13036 | | > show #!63 models |
13037 | | |
13038 | | > show #!60 models |
13039 | | |
13040 | | > show #!61 models |
13041 | | |
13042 | | > show #!62 models |
13043 | | |
13044 | | > show #!51 models |
13045 | | |
13046 | | > show #!52 models |
13047 | | |
13048 | | > save "/Users/becca/Desktop/Postdoc/COPI notebooks and |
13049 | | > resources/Processing/W20_MM_cyto_COPI/20250704_W20_COPI_cyto_alignments.cxs" |
13050 | | |
13051 | | ——— End of log from Fri Jul 4 14:29:17 2025 ——— |
13052 | | |
13053 | | > view name session-start |
13054 | | |
13055 | | opened ChimeraX session |
13056 | | |
13057 | | > hide #!65 models |
13058 | | |
13059 | | > hide #!63 models |
13060 | | |
13061 | | > show #!65 models |
13062 | | |
13063 | | > hide #!65 models |
13064 | | |
13065 | | > show #!63 models |
13066 | | |
13067 | | > show #!65 models |
13068 | | |
13069 | | > combine #60 #61 #62 |
13070 | | |
13071 | | Remapping chain ID 'A' in copy of copy of copy of A_fixed_model_2_A.pdb #61 to |
13072 | | 'B' |
13073 | | Remapping chain ID 'A' in copy of copy of A_fixed_model_2_A.pdb #62 to 'C' |
13074 | | |
13075 | | > rename #64 alpha-COPI |
13076 | | |
13077 | | > hide #!64 models |
13078 | | |
13079 | | > show #!64 models |
13080 | | |
13081 | | > hide #!64 models |
13082 | | |
13083 | | > show #!64 models |
13084 | | |
13085 | | > hide #!64 models |
13086 | | |
13087 | | > hide #!62 models |
13088 | | |
13089 | | > hide #!61 models |
13090 | | |
13091 | | > hide #!60 models |
13092 | | |
13093 | | > show #!64 models |
13094 | | |
13095 | | > combine #51 #52 |
13096 | | |
13097 | | Remapping chain ID 'B' in B_fixed_model_2_B.pdb #52 to 'C' |
13098 | | |
13099 | | > rename #66 beta-COPI |
13100 | | |
13101 | | > hide #!66 models |
13102 | | |
13103 | | > show #!66 models |
13104 | | |
13105 | | > hide #!66 models |
13106 | | |
13107 | | > show #!66 models |
13108 | | |
13109 | | > hide #!66 models |
13110 | | |
13111 | | > hide #!64 models |
13112 | | |
13113 | | > show #!64 models |
13114 | | |
13115 | | > hide #!52 models |
13116 | | |
13117 | | > hide #!51 models |
13118 | | |
13119 | | > hide #!65 models |
13120 | | |
13121 | | > hide #!63 models |
13122 | | |
13123 | | > hide #!57 models |
13124 | | |
13125 | | > hide #!53 models |
13126 | | |
13127 | | > show #!53 models |
13128 | | |
13129 | | > hide #!53 models |
13130 | | |
13131 | | > show #!65 models |
13132 | | |
13133 | | > hide #!65 models |
13134 | | |
13135 | | > show #!63 models |
13136 | | |
13137 | | > show #!53 models |
13138 | | |
13139 | | > hide #!53 models |
13140 | | |
13141 | | > show #!57 models |
13142 | | |
13143 | | > show #!53 models |
13144 | | |
13145 | | > hide #!53 models |
13146 | | |
13147 | | > show #!65 models |
13148 | | |
13149 | | > show #!53 models |
13150 | | |
13151 | | > combine #63 #57 #65 #53 |
13152 | | |
13153 | | Remapping chain ID 'A' in copy of CopBprime_O55029.pdb #57 to 'B' |
13154 | | Remapping chain ID 'A' in copy of copy of CopBprime_O55029.pdb #65 to 'C' |
13155 | | Remapping chain ID 'A' in copy of CopBprime_O55029.pdb #53 to 'D' |
13156 | | |
13157 | | > rename #67 betaprime-COPI |
13158 | | |
13159 | | > hide #!53 models |
13160 | | |
13161 | | > hide #!57 models |
13162 | | |
13163 | | > hide #!63 models |
13164 | | |
13165 | | > hide #!65 models |
13166 | | |
13167 | | > show #!66 models |
13168 | | |
13169 | | > save "/Users/becca/Desktop/Postdoc/COPI notebooks and |
13170 | | > resources/Processing/W20_MM_cyto_COPI/20250704_W20_COPI_cyto_alignments.cxs" |
13171 | | |
13172 | | > show #!37 models |
13173 | | |
13174 | | > show #!38 models |
13175 | | |
13176 | | > hide #!38 models |
13177 | | |
13178 | | > hide #!37 models |
13179 | | |
13180 | | > show #!50.8 models |
13181 | | |
13182 | | > show #50.9 models |
13183 | | |
13184 | | > hide #!64 models |
13185 | | |
13186 | | > hide #!66 models |
13187 | | |
13188 | | > hide #!67 models |
13189 | | |
13190 | | > select add #50.9 |
13191 | | |
13192 | | 319 atoms, 320 bonds, 37 residues, 1 model selected |
13193 | | |
13194 | | > fitmap #50.9 inMap #29.4 |
13195 | | |
13196 | | Fit molecule W20_C1_initial_model_no_wat_lig.pdb A8 (#50.9) to map |
13197 | | W20_J140_run_body004.mrc (#29.4) using 319 atoms |
13198 | | average map value = 0.01618, steps = 332 |
13199 | | shifted from previous position = 5.67 |
13200 | | rotated from previous position = 32.6 degrees |
13201 | | atoms outside contour = 181, contour level = 0.012962 |
13202 | | |
13203 | | Position of W20_C1_initial_model_no_wat_lig.pdb A8 (#50.9) relative to |
13204 | | W20_J140_run_body004.mrc (#29.4) coordinates: |
13205 | | Matrix rotation and translation |
13206 | | -0.96507080 0.08189036 0.24886207 278.79886608 |
13207 | | -0.17377809 -0.91094231 -0.37414608 529.42927332 |
13208 | | 0.19606003 -0.40432423 0.89335457 -19.89239692 |
13209 | | Axis -0.11483207 0.20091912 -0.97285410 |
13210 | | Axis point 157.88995888 252.84037243 0.00000000 |
13211 | | Rotation angle (degrees) 172.44942844 |
13212 | | Shift along axis 93.70981152 |
13213 | | |
13214 | | |
13215 | | > select subtract #50.9 |
13216 | | |
13217 | | Nothing selected |
13218 | | |
13219 | | > combine #37 |
13220 | | |
13221 | | [Repeated 1 time(s)] |
13222 | | |
13223 | | > hide #!69 models |
13224 | | |
13225 | | > hide #50.9 models |
13226 | | |
13227 | | > hide #!29 models |
13228 | | |
13229 | | > ui mousemode right select |
13230 | | |
13231 | | Drag select of 11 residues |
13232 | | |
13233 | | > select up |
13234 | | |
13235 | | 319 atoms, 320 bonds, 37 residues, 1 model selected |
13236 | | |
13237 | | > delete sel |
13238 | | |
13239 | | > fitmap #68 inMap #29.1 |
13240 | | |
13241 | | Fit molecule copy of CopD_full_Q5XJY5.pdb (#68) to map |
13242 | | W20_J140_run_body001.mrc (#29.1) using 1112 atoms |
13243 | | average map value = 0.01419, steps = 64 |
13244 | | shifted from previous position = 0.82 |
13245 | | rotated from previous position = 2.64 degrees |
13246 | | atoms outside contour = 514, contour level = 0.01322 |
13247 | | |
13248 | | Position of copy of CopD_full_Q5XJY5.pdb (#68) relative to |
13249 | | W20_J140_run_body001.mrc (#29.1) coordinates: |
13250 | | Matrix rotation and translation |
13251 | | -0.11662970 -0.98563516 0.12215086 180.96364645 |
13252 | | -0.16309929 -0.10231295 -0.98129031 185.65988338 |
13253 | | 0.97969185 -0.13437031 -0.14882367 140.80476056 |
13254 | | Axis 0.58040695 -0.58768567 0.56369614 |
13255 | | Axis point 145.90273812 0.00000000 210.04228211 |
13256 | | Rotation angle (degrees) 133.14783703 |
13257 | | Shift along axis 75.29400436 |
13258 | | |
13259 | | |
13260 | | > hide #!68 models |
13261 | | |
13262 | | > hide #!50.8 models |
13263 | | |
13264 | | > show #50.9 models |
13265 | | |
13266 | | > show #!69 models |
13267 | | |
13268 | | > ui tool show Matchmaker |
13269 | | |
13270 | | > matchmaker #!69 to #50.9 |
13271 | | |
13272 | | Parameters |
13273 | | --- |
13274 | | Chain pairing | bb |
13275 | | Alignment algorithm | Needleman-Wunsch |
13276 | | Similarity matrix | BLOSUM-62 |
13277 | | SS fraction | 0.3 |
13278 | | Gap open (HH/SS/other) | 18/18/6 |
13279 | | Gap extend | 1 |
13280 | | SS matrix | | | H | S | O |
13281 | | ---|---|---|--- |
13282 | | H | 6 | -9 | -6 |
13283 | | S | | 6 | -6 |
13284 | | O | | | 4 |
13285 | | Iteration cutoff | 2 |
13286 | | |
13287 | | Matchmaker W20_C1_initial_model_no_wat_lig.pdb A8, chain A8 (#50.9) with copy |
13288 | | of CopD_full_Q5XJY5.pdb, chain A (#69), sequence alignment score = 189.1 |
13289 | | RMSD between 37 pruned atom pairs is 0.000 angstroms; (across all 37 pairs: |
13290 | | 0.000) |
13291 | | |
13292 | | Drag select of 135 residues |
13293 | | |
13294 | | > select up |
13295 | | |
13296 | | 1112 atoms, 1129 bonds, 138 residues, 1 model selected |
13297 | | |
13298 | | > delete sel |
13299 | | |
13300 | | > show #!68 models |
13301 | | |
13302 | | > hide #50.9 models |
13303 | | |
13304 | | > select clear |
13305 | | |
13306 | | [Repeated 1 time(s)] |
13307 | | |
13308 | | > select #68/A:138 |
13309 | | |
13310 | | 6 atoms, 5 bonds, 1 residue, 1 model selected |
13311 | | |
13312 | | > show sel atoms |
13313 | | |
13314 | | > style sel stick |
13315 | | |
13316 | | Changed 6 atom styles |
13317 | | |
13318 | | > hide sel cartoons |
13319 | | |
13320 | | > color sel byhetero |
13321 | | |
13322 | | > select #69/A:139 |
13323 | | |
13324 | | 10 atoms, 10 bonds, 1 residue, 1 model selected |
13325 | | |
13326 | | > show sel atoms |
13327 | | |
13328 | | > hide sel cartoons |
13329 | | |
13330 | | > color sel byhetero |
13331 | | |
13332 | | > select clear |
13333 | | |
13334 | | > ui tool show "Build Structure" |
13335 | | |
13336 | | > select #68/A:138@C |
13337 | | |
13338 | | 1 atom, 1 residue, 1 model selected |
13339 | | |
13340 | | > select add #69/A:139@N |
13341 | | |
13342 | | 2 atoms, 2 residues, 2 models selected |
13343 | | |
13344 | | > build join peptide sel length 1.33 omega 180 phi -120 move small |
13345 | | |
13346 | | > undo |
13347 | | |
13348 | | Undo failed, probably because structures have been modified. |
13349 | | |
13350 | | > show #!29 models |
13351 | | |
13352 | | > build join peptide sel length 1.33 omega 90 phi -120 move small |
13353 | | |
13354 | | No chain-terminal carbons in atoms |
13355 | | |
13356 | | > hide #!29 models |
13357 | | |
13358 | | Must select exactly 3 atoms in graphics window |
13359 | | |
13360 | | > select #68/A:138@CA |
13361 | | |
13362 | | 1 atom, 1 residue, 1 model selected |
13363 | | |
13364 | | > select #68/A:138@C |
13365 | | |
13366 | | 1 atom, 1 residue, 1 model selected |
13367 | | |
13368 | | > select add #68/A:138@CA |
13369 | | |
13370 | | 2 atoms, 1 residue, 1 model selected |
13371 | | |
13372 | | > select add #68/A:139@N |
13373 | | |
13374 | | 3 atoms, 2 residues, 1 model selected |
13375 | | Cannot set the angle if the end atoms have a connection that does not pass |
13376 | | through the center atom |
13377 | | |
13378 | | > select clear |
13379 | | |
13380 | | > select #68/A:138@CA |
13381 | | |
13382 | | 1 atom, 1 residue, 1 model selected |
13383 | | |
13384 | | > select add #68/A:138@C |
13385 | | |
13386 | | 2 atoms, 1 residue, 1 model selected |
13387 | | |
13388 | | > select add #68/A:139@N |
13389 | | |
13390 | | 3 atoms, 2 residues, 1 model selected |
13391 | | |
13392 | | > show #!29 models |
13393 | | |
13394 | | > angle #68:138@CA@C:139@N 137.7 |
13395 | | |
13396 | | > angle #68:138@CA@C:139@N 91 |
13397 | | |
13398 | | > angle #68:138@CA@C:139@N 67.2 |
13399 | | |
13400 | | > angle #68:138@CA@C:139@N 110.2 |
13401 | | |
13402 | | > select clear |
13403 | | |
13404 | | > select #68/A:152 |
13405 | | |
13406 | | 9 atoms, 8 bonds, 1 residue, 1 model selected |
13407 | | |
13408 | | > select up |
13409 | | |
13410 | | 319 atoms, 320 bonds, 37 residues, 1 model selected |
13411 | | |
13412 | | > ui mousemode right "translate selected atoms" |
13413 | | |
13414 | | > select add #68 |
13415 | | |
13416 | | 1431 atoms, 1450 bonds, 175 residues, 1 model selected |
13417 | | |
13418 | | > select subtract #68 |
13419 | | |
13420 | | Nothing selected |
13421 | | |
13422 | | > ui mousemode right tug |
13423 | | |
13424 | | > ui mousemode right select |
13425 | | |
13426 | | > select #68/A:155 |
13427 | | |
13428 | | 5 atoms, 4 bonds, 1 residue, 1 model selected |
13429 | | |
13430 | | > select up |
13431 | | |
13432 | | 319 atoms, 320 bonds, 37 residues, 1 model selected |
13433 | | |
13434 | | > ui mousemode right tug |
13435 | | |
13436 | | > ui mousemode right "bond rotation" |
13437 | | |
13438 | | > ui mousemode right select |
13439 | | |
13440 | | > ui mousemode right "play coordinates" |
13441 | | |
13442 | | > select add #68 |
13443 | | |
13444 | | 1431 atoms, 1450 bonds, 175 residues, 1 model selected |
13445 | | |
13446 | | > select subtract #68 |
13447 | | |
13448 | | Nothing selected |
13449 | | |
13450 | | > hide #!68 models |
13451 | | |
13452 | | > close #68 |
13453 | | |
13454 | | > combine 37 |
13455 | | |
13456 | | Expected a keyword |
13457 | | |
13458 | | > combine #37 |
13459 | | |
13460 | | > delete sel |
13461 | | |
13462 | | > combine #37 |
13463 | | |
13464 | | > hide #!69 models |
13465 | | |
13466 | | > hide #!29 models |
13467 | | |
13468 | | > ui mousemode right select |
13469 | | |
13470 | | Drag select of 7 residues |
13471 | | |
13472 | | > select up |
13473 | | |
13474 | | 319 atoms, 320 bonds, 37 residues, 1 model selected |
13475 | | |
13476 | | > combine #37 |
13477 | | |
13478 | | > delete sel |
13479 | | |
13480 | | > close #70 |
13481 | | |
13482 | | > hide #!68 models |
13483 | | |
13484 | | > ui tool show Matchmaker |
13485 | | |
13486 | | > matchmaker #!69 to #50.9 |
13487 | | |
13488 | | Parameters |
13489 | | --- |
13490 | | Chain pairing | bb |
13491 | | Alignment algorithm | Needleman-Wunsch |
13492 | | Similarity matrix | BLOSUM-62 |
13493 | | SS fraction | 0.3 |
13494 | | Gap open (HH/SS/other) | 18/18/6 |
13495 | | Gap extend | 1 |
13496 | | SS matrix | | | H | S | O |
13497 | | ---|---|---|--- |
13498 | | H | 6 | -9 | -6 |
13499 | | S | | 6 | -6 |
13500 | | O | | | 4 |
13501 | | Iteration cutoff | 2 |
13502 | | |
13503 | | Matchmaker W20_C1_initial_model_no_wat_lig.pdb A8, chain A8 (#50.9) with copy |
13504 | | of CopD_full_Q5XJY5.pdb, chain A (#69), sequence alignment score = 189.1 |
13505 | | RMSD between 37 pruned atom pairs is 0.000 angstroms; (across all 37 pairs: |
13506 | | 0.000) |
13507 | | |
13508 | | |
13509 | | > show #!69 models |
13510 | | |
13511 | | Drag select of 103 residues |
13512 | | |
13513 | | > select up |
13514 | | |
13515 | | 936 atoms, 951 bonds, 116 residues, 1 model selected |
13516 | | Drag select of 135 residues |
13517 | | |
13518 | | > select up |
13519 | | |
13520 | | 1112 atoms, 1129 bonds, 138 residues, 1 model selected |
13521 | | |
13522 | | > delete sel |
13523 | | |
13524 | | > show #!29 models |
13525 | | |
13526 | | > show #!68 models |
13527 | | |
13528 | | > hide #!29 models |
13529 | | |
13530 | | > select #69/A:168 |
13531 | | |
13532 | | 9 atoms, 8 bonds, 1 residue, 1 model selected |
13533 | | |
13534 | | > select clear |
13535 | | |
13536 | | > select #69/A:165 |
13537 | | |
13538 | | 9 atoms, 8 bonds, 1 residue, 1 model selected |
13539 | | |
13540 | | > select clear |
13541 | | |
13542 | | > select #69/A:162 |
13543 | | |
13544 | | 9 atoms, 8 bonds, 1 residue, 1 model selected |
13545 | | |
13546 | | > show sel atoms |
13547 | | |
13548 | | > select #69/A:164 |
13549 | | |
13550 | | 9 atoms, 8 bonds, 1 residue, 1 model selected |
13551 | | |
13552 | | > show sel atoms |
13553 | | |
13554 | | > show #!29 models |
13555 | | |
13556 | | > hide #!29 models |
13557 | | |
13558 | | > show #!30 models |
13559 | | |
13560 | | > select #69/A:165 |
13561 | | |
13562 | | 9 atoms, 8 bonds, 1 residue, 1 model selected |
13563 | | |
13564 | | > show sel atoms |
13565 | | |
13566 | | > color #30.1 #92929280 models |
13567 | | |
13568 | | > color #30.4 #92929263 models |
13569 | | |
13570 | | > show #!67 models |
13571 | | |
13572 | | > select add #69 |
13573 | | |
13574 | | 319 atoms, 320 bonds, 37 residues, 1 model selected |
13575 | | |
13576 | | > hide sel atoms |
13577 | | |
13578 | | > select subtract #69 |
13579 | | |
13580 | | Nothing selected |
13581 | | |
13582 | | > combine #68 #69 |
13583 | | |
13584 | | Remapping chain ID 'A' in copy of CopD_full_Q5XJY5.pdb #69 to 'B' |
13585 | | |
13586 | | > rename #70 delta-COPI |
13587 | | |
13588 | | > hide #!69 models |
13589 | | |
13590 | | > hide #!68 models |
13591 | | |
13592 | | > hide #!30.1 models |
13593 | | |
13594 | | > show #!30.1 models |
13595 | | |
13596 | | > hide #!30 models |
13597 | | |
13598 | | > show #!29 models |
13599 | | |
13600 | | > show #!66 models |
13601 | | |
13602 | | > show #!64 models |
13603 | | |
13604 | | > show #39 models |
13605 | | |
13606 | | > hide #!29 models |
13607 | | |
13608 | | > show #!33 models |
13609 | | |
13610 | | > hide #!33 models |
13611 | | |
13612 | | > show #!32 models |
13613 | | |
13614 | | > hide #!32 models |
13615 | | |
13616 | | > show #!33 models |
13617 | | |
13618 | | > fitmap #39 inMap #33.3 |
13619 | | |
13620 | | Fit molecule CopZ1_P61924.pdb (#39) to map W20_J160_run_ct6_body003.mrc |
13621 | | (#33.3) using 1420 atoms |
13622 | | average map value = 0.0138, steps = 80 |
13623 | | shifted from previous position = 0.00922 |
13624 | | rotated from previous position = 0.0243 degrees |
13625 | | atoms outside contour = 767, contour level = 0.015167 |
13626 | | |
13627 | | Position of CopZ1_P61924.pdb (#39) relative to W20_J160_run_ct6_body003.mrc |
13628 | | (#33.3) coordinates: |
13629 | | Matrix rotation and translation |
13630 | | -0.59905768 0.35670401 0.71686272 128.55067540 |
13631 | | 0.33940715 -0.69775395 0.63082663 127.78309606 |
13632 | | 0.72521218 0.62120986 0.29692697 93.14263724 |
13633 | | Axis -0.44771433 -0.38871406 -0.80526596 |
13634 | | Axis point 38.57235221 41.25427436 0.00000000 |
13635 | | Rotation angle (degrees) 179.38464020 |
13636 | | Shift along axis -182.22965962 |
13637 | | |
13638 | | |
13639 | | > hide #39 models |
13640 | | |
13641 | | > show #39 models |
13642 | | |
13643 | | > combine #39 |
13644 | | |
13645 | | > rename #71 zeta-COPI |
13646 | | |
13647 | | > show #56 models |
13648 | | |
13649 | | > hide #56 models |
13650 | | |
13651 | | > show #!55 models |
13652 | | |
13653 | | > hide #!55 models |
13654 | | |
13655 | | > show #!38 models |
13656 | | |
13657 | | > save "/Users/becca/Desktop/Postdoc/COPI notebooks and |
13658 | | > resources/Processing/W20_MM_cyto_COPI/20250704_W20_COPI_cyto_alignments.cxs" |
13659 | | |
13660 | | > hide #!38 models |
13661 | | |
13662 | | > show #!50.4 models |
13663 | | |
13664 | | > hide #!50.4 models |
13665 | | |
13666 | | > show #!50.4 models |
13667 | | |
13668 | | > hide #!50.4 models |
13669 | | |
13670 | | > show #!50.4 models |
13671 | | |
13672 | | > hide #!50.4 models |
13673 | | |
13674 | | > show #50.1 models |
13675 | | |
13676 | | > show #!50.2 models |
13677 | | |
13678 | | > hide #!50.2 models |
13679 | | |
13680 | | > show #!50.3 models |
13681 | | |
13682 | | > hide #!50.3 models |
13683 | | |
13684 | | > show #!50.4 models |
13685 | | |
13686 | | > hide #!50.4 models |
13687 | | |
13688 | | > show #!50.5 models |
13689 | | |
13690 | | > hide #!50.5 models |
13691 | | |
13692 | | > show #50.6 models |
13693 | | |
13694 | | > hide #50.6 models |
13695 | | |
13696 | | > show #!50.7 models |
13697 | | |
13698 | | > hide #!50.7 models |
13699 | | |
13700 | | > show #!50.8 models |
13701 | | |
13702 | | > hide #!50.8 models |
13703 | | |
13704 | | > show #50.9 models |
13705 | | |
13706 | | > hide #50.9 models |
13707 | | |
13708 | | > show #!50.10 models |
13709 | | |
13710 | | > select add #50.10 |
13711 | | |
13712 | | 4143 atoms, 4205 bonds, 2 pseudobonds, 528 residues, 2 models selected |
13713 | | Drag select of 33.3 W20_J160_run_ct6_body003.mrc , 4 residues |
13714 | | |
13715 | | > select add #50.10 |
13716 | | |
13717 | | 4143 atoms, 4205 bonds, 2 pseudobonds, 528 residues, 4 models selected |
13718 | | |
13719 | | > ui mousemode right "translate selected models" |
13720 | | |
13721 | | > view matrix models |
13722 | | > #33.3,0.99304,0.059881,0.10144,5.194,-0.064889,0.99679,0.046802,13.668,-0.098315,-0.053059,0.99374,40.329,#50.10,-0.92962,0.23756,-0.28172,353.7,-0.021762,-0.79854,-0.60155,498.49,-0.36787,-0.55308,0.74751,143.01 |
13723 | | |
13724 | | > undo |
13725 | | |
13726 | | [Repeated 1 time(s)] |
13727 | | |
13728 | | > ui mousemode right select |
13729 | | |
13730 | | > select clear |
13731 | | |
13732 | | > open "/Users/becca/Desktop/Postdoc/COPI notebooks and resources/Structure |
13733 | | > files/alphafold/CopG1_ Q9QZE5.pdb" |
13734 | | |
13735 | | CopG1_ Q9QZE5.pdb title: |
13736 | | Alphafold monomer V2.0 prediction for coatomer subunit γ-1 (Q9QZE5) [more |
13737 | | info...] |
13738 | | |
13739 | | Chain information for CopG1_ Q9QZE5.pdb #72 |
13740 | | --- |
13741 | | Chain | Description | UniProt |
13742 | | A | coatomer subunit γ-1 | COPG1_MOUSE 1-874 |
13743 | | |
13744 | | Computing secondary structure |
13745 | | |
13746 | | > select add #72 |
13747 | | |
13748 | | 6830 atoms, 6947 bonds, 874 residues, 1 model selected |
13749 | | |
13750 | | > ui tool show Matchmaker |
13751 | | |
13752 | | > matchmaker #72 to #50.10 |
13753 | | |
13754 | | Parameters |
13755 | | --- |
13756 | | Chain pairing | bb |
13757 | | Alignment algorithm | Needleman-Wunsch |
13758 | | Similarity matrix | BLOSUM-62 |
13759 | | SS fraction | 0.3 |
13760 | | Gap open (HH/SS/other) | 18/18/6 |
13761 | | Gap extend | 1 |
13762 | | SS matrix | | | H | S | O |
13763 | | ---|---|---|--- |
13764 | | H | 6 | -9 | -6 |
13765 | | S | | 6 | -6 |
13766 | | O | | | 4 |
13767 | | Iteration cutoff | 2 |
13768 | | |
13769 | | Matchmaker W20_C1_initial_model_no_wat_lig.pdb A9, chain A9 (#50.10) with |
13770 | | CopG1_ Q9QZE5.pdb, chain A (#72), sequence alignment score = 2728.3 |
13771 | | RMSD between 527 pruned atom pairs is 0.000 angstroms; (across all 528 pairs: |
13772 | | 0.471) |
13773 | | |
13774 | | |
13775 | | > select subtract #72 |
13776 | | |
13777 | | Nothing selected |
13778 | | |
13779 | | > hide #!64 models |
13780 | | |
13781 | | > hide #!66 models |
13782 | | |
13783 | | > hide #!67 models |
13784 | | |
13785 | | > hide #!70 models |
13786 | | |
13787 | | > hide #71 models |
13788 | | |
13789 | | > hide #39 models |
13790 | | |
13791 | | > color #72 gammav3 |
13792 | | |
13793 | | > fitmap #72 inMap #33.3 |
13794 | | |
13795 | | Fit molecule CopG1_ Q9QZE5.pdb (#72) to map W20_J160_run_ct6_body003.mrc |
13796 | | (#33.3) using 6830 atoms |
13797 | | average map value = 0.008592, steps = 128 |
13798 | | shifted from previous position = 5.94 |
13799 | | rotated from previous position = 20.6 degrees |
13800 | | atoms outside contour = 5149, contour level = 0.015167 |
13801 | | |
13802 | | Position of CopG1_ Q9QZE5.pdb (#72) relative to W20_J160_run_ct6_body003.mrc |
13803 | | (#33.3) coordinates: |
13804 | | Matrix rotation and translation |
13805 | | -0.21262269 0.89025762 0.40278153 115.23719127 |
13806 | | -0.39923844 0.29708069 -0.86738211 108.04417936 |
13807 | | -0.89185215 -0.34523099 0.29225897 114.80605117 |
13808 | | Axis 0.27475861 0.68124291 -0.67853946 |
13809 | | Axis point 106.74618671 0.00000000 53.93689380 |
13810 | | Rotation angle (degrees) 108.15818379 |
13811 | | Shift along axis 27.36630527 |
13812 | | |
13813 | | |
13814 | | > hide #!50.10 models |
13815 | | |
13816 | | > hide #50.1 models |
13817 | | |
13818 | | > hide #72 models |
13819 | | |
13820 | | > show #!41 models |
13821 | | |
13822 | | > show #40 models |
13823 | | |
13824 | | > hide #40 models |
13825 | | |
13826 | | > hide #!41 models |
13827 | | |
13828 | | > open "/Users/becca/Desktop/Postdoc/COPI notebooks and resources/Alphafold |
13829 | | > predictions/alphafold 3 |
13830 | | > /fold_20240724_copbdg1z1_try2/fold_20240724_copbdg1z1_try2_model_0.cif" |
13831 | | |
13832 | | Chain information for fold_20240724_copbdg1z1_try2_model_0.cif #73 |
13833 | | --- |
13834 | | Chain | Description |
13835 | | A | . |
13836 | | B | . |
13837 | | C | . |
13838 | | D | . |
13839 | | |
13840 | | Computing secondary structure |
13841 | | |
13842 | | > ui tool show Matchmaker |
13843 | | |
13844 | | > matchmaker #73 to #72 |
13845 | | |
13846 | | Parameters |
13847 | | --- |
13848 | | Chain pairing | bb |
13849 | | Alignment algorithm | Needleman-Wunsch |
13850 | | Similarity matrix | BLOSUM-62 |
13851 | | SS fraction | 0.3 |
13852 | | Gap open (HH/SS/other) | 18/18/6 |
13853 | | Gap extend | 1 |
13854 | | SS matrix | | | H | S | O |
13855 | | ---|---|---|--- |
13856 | | H | 6 | -9 | -6 |
13857 | | S | | 6 | -6 |
13858 | | O | | | 4 |
13859 | | Iteration cutoff | 2 |
13860 | | |
13861 | | Matchmaker CopG1_ Q9QZE5.pdb, chain A (#72) with |
13862 | | fold_20240724_copbdg1z1_try2_model_0.cif, chain C (#73), sequence alignment |
13863 | | score = 4461 |
13864 | | RMSD between 219 pruned atom pairs is 1.225 angstroms; (across all 874 pairs: |
13865 | | 21.353) |
13866 | | |
13867 | | |
13868 | | > hide #!50 models |
13869 | | |
13870 | | > show #!50 models |
13871 | | |
13872 | | > hide #!33 models |
13873 | | |
13874 | | > select clear |
13875 | | |
13876 | | > select #73/B:154 |
13877 | | |
13878 | | 9 atoms, 8 bonds, 1 residue, 1 model selected |
13879 | | |
13880 | | > select up |
13881 | | |
13882 | | 319 atoms, 320 bonds, 37 residues, 1 model selected |
13883 | | |
13884 | | > select up |
13885 | | |
13886 | | 4014 atoms, 4083 bonds, 511 residues, 1 model selected |
13887 | | |
13888 | | > delete sel |
13889 | | |
13890 | | > select #73/A:242 |
13891 | | |
13892 | | 11 atoms, 10 bonds, 1 residue, 1 model selected |
13893 | | |
13894 | | > select up |
13895 | | |
13896 | | 49 atoms, 49 bonds, 6 residues, 1 model selected |
13897 | | |
13898 | | > select up |
13899 | | |
13900 | | 7501 atoms, 7619 bonds, 953 residues, 1 model selected |
13901 | | |
13902 | | > delete sel |
13903 | | |
13904 | | > fitmap #73 inMap #33.3 |
13905 | | |
13906 | | Fit molecule fold_20240724_copbdg1z1_try2_model_0.cif (#73) to map |
13907 | | W20_J160_run_ct6_body003.mrc (#33.3) using 8250 atoms |
13908 | | average map value = 0.008274, steps = 184 |
13909 | | shifted from previous position = 16.4 |
13910 | | rotated from previous position = 27.6 degrees |
13911 | | atoms outside contour = 6288, contour level = 0.015167 |
13912 | | |
13913 | | Position of fold_20240724_copbdg1z1_try2_model_0.cif (#73) relative to |
13914 | | W20_J160_run_ct6_body003.mrc (#33.3) coordinates: |
13915 | | Matrix rotation and translation |
13916 | | 0.89001705 -0.00769161 -0.45586235 140.75280440 |
13917 | | 0.39379749 -0.49091643 0.77712585 125.72122596 |
13918 | | -0.22976767 -0.87117271 -0.43389506 123.79108229 |
13919 | | Axis -0.96307771 -0.13210395 0.23458445 |
13920 | | Axis point 0.00000000 97.90843398 46.99023466 |
13921 | | Rotation angle (degrees) 121.15782367 |
13922 | | Shift along axis -123.12469702 |
13923 | | |
13924 | | |
13925 | | > show #!33 models |
13926 | | |
13927 | | > show #71 models |
13928 | | |
13929 | | > hide #71 models |
13930 | | |
13931 | | > show #71 models |
13932 | | |
13933 | | > hide #71 models |
13934 | | |
13935 | | > show #71 models |
13936 | | |
13937 | | > hide #71 models |
13938 | | |
13939 | | > hide #!33 models |
13940 | | |
13941 | | > select #73/D:47 |
13942 | | |
13943 | | 8 atoms, 7 bonds, 1 residue, 1 model selected |
13944 | | |
13945 | | > select up |
13946 | | |
13947 | | 128 atoms, 129 bonds, 15 residues, 1 model selected |
13948 | | |
13949 | | > select up |
13950 | | |
13951 | | 1420 atoms, 1441 bonds, 177 residues, 1 model selected |
13952 | | |
13953 | | > delete sel |
13954 | | |
13955 | | > show #!33 models |
13956 | | |
13957 | | > fitmap #73 inMap #33.3 |
13958 | | |
13959 | | Fit molecule fold_20240724_copbdg1z1_try2_model_0.cif (#73) to map |
13960 | | W20_J160_run_ct6_body003.mrc (#33.3) using 6830 atoms |
13961 | | average map value = 0.007849, steps = 80 |
13962 | | shifted from previous position = 1.21 |
13963 | | rotated from previous position = 2.15 degrees |
13964 | | atoms outside contour = 5293, contour level = 0.015167 |
13965 | | |
13966 | | Position of fold_20240724_copbdg1z1_try2_model_0.cif (#73) relative to |
13967 | | W20_J160_run_ct6_body003.mrc (#33.3) coordinates: |
13968 | | Matrix rotation and translation |
13969 | | 0.89436621 -0.03216667 -0.44617753 141.60252778 |
13970 | | 0.36827977 -0.51322863 0.77522022 126.38957440 |
13971 | | -0.25392733 -0.85764892 -0.44716801 122.18980164 |
13972 | | Axis -0.96493218 -0.11360886 0.23664092 |
13973 | | Axis point 0.00000000 98.06597121 46.27860492 |
13974 | | Rotation angle (degrees) 122.20940815 |
13975 | | Shift along axis -122.08070325 |
13976 | | |
13977 | | |
13978 | | > show #35 models |
13979 | | |
13980 | | > close #73 |
13981 | | |
13982 | | > open "/Users/becca/Desktop/Postdoc/COPI notebooks and resources/Alphafold |
13983 | | > predictions/alphafold 3 |
13984 | | > /fold_20240711_copi_golph3/fold_20240711_copi_golph3_model_0.cif" |
13985 | | |
13986 | | Chain information for fold_20240711_copi_golph3_model_0.cif #73 |
13987 | | --- |
13988 | | Chain | Description |
13989 | | A | . |
13990 | | B C | . |
13991 | | D | . |
13992 | | E | . |
13993 | | F | . |
13994 | | G | . |
13995 | | H | . |
13996 | | I | . |
13997 | | |
13998 | | |
13999 | | > ui tool show Matchmaker |
14000 | | |
14001 | | > matchmaker #73 to #72 |
14002 | | |
14003 | | Computing secondary structure |
14004 | | Parameters |
14005 | | --- |
14006 | | Chain pairing | bb |
14007 | | Alignment algorithm | Needleman-Wunsch |
14008 | | Similarity matrix | BLOSUM-62 |
14009 | | SS fraction | 0.3 |
14010 | | Gap open (HH/SS/other) | 18/18/6 |
14011 | | Gap extend | 1 |
14012 | | SS matrix | | | H | S | O |
14013 | | ---|---|---|--- |
14014 | | H | 6 | -9 | -6 |
14015 | | S | | 6 | -6 |
14016 | | O | | | 4 |
14017 | | Iteration cutoff | 2 |
14018 | | |
14019 | | Matchmaker CopG1_ Q9QZE5.pdb, chain A (#72) with |
14020 | | fold_20240711_copi_golph3_model_0.cif, chain H (#73), sequence alignment score |
14021 | | = 3086.5 |
14022 | | RMSD between 216 pruned atom pairs is 1.190 angstroms; (across all 609 pairs: |
14023 | | 15.751) |
14024 | | |
14025 | | |
14026 | | > hide #35,73 atoms |
14027 | | |
14028 | | > hide #35,73 cartoons |
14029 | | |
14030 | | > show #35,73 cartoons |
14031 | | |
14032 | | > hide #35 models |
14033 | | |
14034 | | > hide #!33 models |
14035 | | |
14036 | | > select #73/C:142 |
14037 | | |
14038 | | 9 atoms, 8 bonds, 1 residue, 1 model selected |
14039 | | |
14040 | | > select up |
14041 | | |
14042 | | 59 atoms, 58 bonds, 8 residues, 1 model selected |
14043 | | |
14044 | | > select up |
14045 | | |
14046 | | 1457 atoms, 1483 bonds, 181 residues, 1 model selected |
14047 | | |
14048 | | > delete sel |
14049 | | |
14050 | | > split #73 chains |
14051 | | |
14052 | | Split fold_20240711_copi_golph3_model_0.cif (#73) into 8 models |
14053 | | Chain information for fold_20240711_copi_golph3_model_0.cif A #73.1 |
14054 | | --- |
14055 | | Chain | Description |
14056 | | A | No description available |
14057 | | |
14058 | | Chain information for fold_20240711_copi_golph3_model_0.cif B #73.2 |
14059 | | --- |
14060 | | Chain | Description |
14061 | | B | No description available |
14062 | | |
14063 | | Chain information for fold_20240711_copi_golph3_model_0.cif D #73.3 |
14064 | | --- |
14065 | | Chain | Description |
14066 | | D | No description available |
14067 | | |
14068 | | Chain information for fold_20240711_copi_golph3_model_0.cif E #73.4 |
14069 | | --- |
14070 | | Chain | Description |
14071 | | E | No description available |
14072 | | |
14073 | | Chain information for fold_20240711_copi_golph3_model_0.cif F #73.5 |
14074 | | --- |
14075 | | Chain | Description |
14076 | | F | No description available |
14077 | | |
14078 | | Chain information for fold_20240711_copi_golph3_model_0.cif G #73.6 |
14079 | | --- |
14080 | | Chain | Description |
14081 | | G | No description available |
14082 | | |
14083 | | Chain information for fold_20240711_copi_golph3_model_0.cif H #73.7 |
14084 | | --- |
14085 | | Chain | Description |
14086 | | H | No description available |
14087 | | |
14088 | | Chain information for fold_20240711_copi_golph3_model_0.cif I #73.8 |
14089 | | --- |
14090 | | Chain | Description |
14091 | | I | No description available |
14092 | | |
14093 | | |
14094 | | > hide #73.1 models |
14095 | | |
14096 | | > show #73.1 models |
14097 | | |
14098 | | > close #73.1 |
14099 | | |
14100 | | > hide #!73 models |
14101 | | |
14102 | | > show #!73 models |
14103 | | |
14104 | | > hide #73.2 models |
14105 | | |
14106 | | > show #73.2 models |
14107 | | |
14108 | | > close #73.2 |
14109 | | |
14110 | | > hide #73.3 models |
14111 | | |
14112 | | > show #73.3 models |
14113 | | |
14114 | | > close #73.3 |
14115 | | |
14116 | | > hide #73.4 models |
14117 | | |
14118 | | > show #73.4 models |
14119 | | |
14120 | | > hide #73.5 models |
14121 | | |
14122 | | > show #73.5 models |
14123 | | |
14124 | | > close #73.5 |
14125 | | |
14126 | | > close #73.6 |
14127 | | |
14128 | | > show #!33 models |
14129 | | |
14130 | | > close #73.4 |
14131 | | |
14132 | | > show #!50.10 models |
14133 | | |
14134 | | > hide #!50.10 models |
14135 | | |
14136 | | > show #50.9 models |
14137 | | |
14138 | | > hide #50.9 models |
14139 | | |
14140 | | > show #50.9 models |
14141 | | |
14142 | | > hide #50.9 models |
14143 | | |
14144 | | > show #50.1 models |
14145 | | |
14146 | | > hide #50.1 models |
14147 | | |
14148 | | > show #71 models |
14149 | | |
14150 | | > close #73.8 |
14151 | | |
14152 | | > combine #73.7 |
14153 | | |
14154 | | > hide #73.7 models |
14155 | | |
14156 | | > hide #!73 models |
14157 | | |
14158 | | > hide #71 models |
14159 | | |
14160 | | > hide #!33 models |
14161 | | |
14162 | | > show #!33 models |
14163 | | |
14164 | | > select #74/H:609 |
14165 | | |
14166 | | 12 atoms, 11 bonds, 1 residue, 1 model selected |
14167 | | |
14168 | | > select up |
14169 | | |
14170 | | 227 atoms, 230 bonds, 31 residues, 1 model selected |
14171 | | |
14172 | | > delete sel |
14173 | | |
14174 | | > select #74/H:564 |
14175 | | |
14176 | | 9 atoms, 8 bonds, 1 residue, 1 model selected |
14177 | | |
14178 | | > select up |
14179 | | |
14180 | | 103 atoms, 104 bonds, 13 residues, 1 model selected |
14181 | | |
14182 | | > select up |
14183 | | |
14184 | | 4525 atoms, 4596 bonds, 578 residues, 1 model selected |
14185 | | |
14186 | | > select clear |
14187 | | |
14188 | | > select #74/H:353 |
14189 | | |
14190 | | 6 atoms, 5 bonds, 1 residue, 1 model selected |
14191 | | |
14192 | | > hide #!33 models |
14193 | | |
14194 | | Drag select of 109 residues |
14195 | | |
14196 | | > select up |
14197 | | |
14198 | | 939 atoms, 952 bonds, 119 residues, 1 model selected |
14199 | | |
14200 | | > delete sel |
14201 | | |
14202 | | Drag select of 27 residues, 2 pseudobonds |
14203 | | |
14204 | | > delete sel |
14205 | | |
14206 | | > fitmap #74 inMap #33.3 |
14207 | | |
14208 | | Fit molecule copy of fold_20240711_copi_golph3_model_0.cif H (#74) to map |
14209 | | W20_J160_run_ct6_body003.mrc (#33.3) using 3367 atoms |
14210 | | average map value = 0.01504, steps = 104 |
14211 | | shifted from previous position = 1.23 |
14212 | | rotated from previous position = 8.87 degrees |
14213 | | atoms outside contour = 1844, contour level = 0.015167 |
14214 | | |
14215 | | Position of copy of fold_20240711_copi_golph3_model_0.cif H (#74) relative to |
14216 | | W20_J160_run_ct6_body003.mrc (#33.3) coordinates: |
14217 | | Matrix rotation and translation |
14218 | | -0.99039171 0.12782412 -0.05277549 148.12639654 |
14219 | | 0.13807487 0.93531006 -0.32577664 131.22576391 |
14220 | | 0.00771933 -0.32993345 -0.94397263 135.30516062 |
14221 | | Axis -0.06759286 -0.98369069 0.16668480 |
14222 | | Axis point 68.34524384 0.00000000 79.86683576 |
14223 | | Rotation angle (degrees) 178.23793985 |
14224 | | Shift along axis -116.54453594 |
14225 | | |
14226 | | |
14227 | | > show #!33 models |
14228 | | |
14229 | | > hide #!33 models |
14230 | | |
14231 | | Drag select of 37 residues |
14232 | | |
14233 | | > select up |
14234 | | |
14235 | | 343 atoms, 348 bonds, 42 residues, 1 model selected |
14236 | | |
14237 | | > delete sel |
14238 | | |
14239 | | > fitmap #74 inMap #33.3 |
14240 | | |
14241 | | Fit molecule copy of fold_20240711_copi_golph3_model_0.cif H (#74) to map |
14242 | | W20_J160_run_ct6_body003.mrc (#33.3) using 3024 atoms |
14243 | | average map value = 0.01541, steps = 84 |
14244 | | shifted from previous position = 0.341 |
14245 | | rotated from previous position = 2.61 degrees |
14246 | | atoms outside contour = 1604, contour level = 0.015167 |
14247 | | |
14248 | | Position of copy of fold_20240711_copi_golph3_model_0.cif H (#74) relative to |
14249 | | W20_J160_run_ct6_body003.mrc (#33.3) coordinates: |
14250 | | Matrix rotation and translation |
14251 | | -0.99016649 0.13961225 -0.00887325 145.93626541 |
14252 | | 0.13499683 0.93694534 -0.32234963 131.14942473 |
14253 | | -0.03669021 -0.32037766 -0.94657909 136.23220850 |
14254 | | Axis 0.06976430 0.98410929 -0.16328456 |
14255 | | Axis point 68.87722497 0.00000000 78.50166031 |
14256 | | Rotation angle (degrees) 179.19020792 |
14257 | | Shift along axis 117.00189352 |
14258 | | |
14259 | | |
14260 | | > show #!33 models |
14261 | | |
14262 | | > hide #!33 models |
14263 | | |
14264 | | Drag select of 7 residues |
14265 | | |
14266 | | > select up |
14267 | | |
14268 | | 130 atoms, 130 bonds, 17 residues, 1 model selected |
14269 | | Drag select of 5 residues |
14270 | | |
14271 | | > select down |
14272 | | |
14273 | | 44 atoms, 5 residues, 1 model selected |
14274 | | |
14275 | | > select up |
14276 | | |
14277 | | 142 atoms, 143 bonds, 18 residues, 1 model selected |
14278 | | |
14279 | | > delete sel |
14280 | | |
14281 | | > fitmap #74 inMap #33.3 |
14282 | | |
14283 | | Fit molecule copy of fold_20240711_copi_golph3_model_0.cif H (#74) to map |
14284 | | W20_J160_run_ct6_body003.mrc (#33.3) using 2882 atoms |
14285 | | average map value = 0.01543, steps = 60 |
14286 | | shifted from previous position = 0.199 |
14287 | | rotated from previous position = 0.43 degrees |
14288 | | atoms outside contour = 1520, contour level = 0.015167 |
14289 | | |
14290 | | Position of copy of fold_20240711_copi_golph3_model_0.cif H (#74) relative to |
14291 | | W20_J160_run_ct6_body003.mrc (#33.3) coordinates: |
14292 | | Matrix rotation and translation |
14293 | | -0.99012237 0.14014794 -0.00403046 145.70159327 |
14294 | | 0.13418777 0.93889954 -0.31695629 130.92767817 |
14295 | | -0.04063657 -0.31436635 -0.94843158 136.20145596 |
14296 | | Axis 0.06966230 0.98460506 -0.16031230 |
14297 | | Axis point 68.95076353 0.00000000 78.11025829 |
14298 | | Rotation angle (degrees) 178.93485378 |
14299 | | Shift along axis 117.22719466 |
14300 | | |
14301 | | |
14302 | | > show #!33 models |
14303 | | |
14304 | | > show #!50.10 models |
14305 | | |
14306 | | > hide #!50 models |
14307 | | |
14308 | | > show #!50 models |
14309 | | |
14310 | | > hide #!33 models |
14311 | | |
14312 | | > combine #50.10 |
14313 | | |
14314 | | > hide #!50.10 models |
14315 | | |
14316 | | > hide #!74 models |
14317 | | |
14318 | | > show #!74 models |
14319 | | |
14320 | | > hide #!74 models |
14321 | | |
14322 | | Drag select of 113 residues |
14323 | | |
14324 | | > select up |
14325 | | |
14326 | | 1028 atoms, 1042 bonds, 130 residues, 1 model selected |
14327 | | |
14328 | | > delete sel |
14329 | | |
14330 | | > fitmap #75 inMap #33.3 |
14331 | | |
14332 | | Fit molecule copy of W20_C1_initial_model_no_wat_lig.pdb A9 (#75) to map |
14333 | | W20_J160_run_ct6_body003.mrc (#33.3) using 3115 atoms |
14334 | | average map value = 0.01389, steps = 132 |
14335 | | shifted from previous position = 1.68 |
14336 | | rotated from previous position = 15.8 degrees |
14337 | | atoms outside contour = 1847, contour level = 0.015167 |
14338 | | |
14339 | | Position of copy of W20_C1_initial_model_no_wat_lig.pdb A9 (#75) relative to |
14340 | | W20_J160_run_ct6_body003.mrc (#33.3) coordinates: |
14341 | | Matrix rotation and translation |
14342 | | -0.96975860 0.09758407 -0.22370877 404.94223752 |
14343 | | 0.05855198 -0.79680528 -0.60139256 482.79146988 |
14344 | | -0.23693866 -0.59630420 0.76699503 131.62487193 |
14345 | | Axis 0.12253380 0.31859145 -0.93993880 |
14346 | | Axis point 213.57688590 261.52023160 0.00000000 |
14347 | | Rotation angle (degrees) 178.81027642 |
14348 | | Shift along axis 79.71302152 |
14349 | | |
14350 | | |
14351 | | > show #!33 models |
14352 | | |
14353 | | > open "/Users/becca/Desktop/Postdoc/Grants and paper drafts/Golph3 |
14354 | | > paper/Final maps and model/Model_files/20250328_COPI_GOLPH3_model_v2.cif" |
14355 | | |
14356 | | Chain information for 20250328_COPI_GOLPH3_model_v2.cif #76 |
14357 | | --- |
14358 | | Chain | Description |
14359 | | A | No description available |
14360 | | B | No description available |
14361 | | D | No description available |
14362 | | E | No description available |
14363 | | F | No description available |
14364 | | G | No description available |
14365 | | H | No description available |
14366 | | I | No description available |
14367 | | J | No description available |
14368 | | K | No description available |
14369 | | L | No description available |
14370 | | M | No description available |
14371 | | N | No description available |
14372 | | O | No description available |
14373 | | P | No description available |
14374 | | R S T | No description available |
14375 | | |
14376 | | |
14377 | | > hide #76#!75 atoms |
14378 | | |
14379 | | > show #76#!75 cartoons |
14380 | | |
14381 | | > ui tool show Matchmaker |
14382 | | |
14383 | | > matchmaker #76 to #66 |
14384 | | |
14385 | | Parameters |
14386 | | --- |
14387 | | Chain pairing | bb |
14388 | | Alignment algorithm | Needleman-Wunsch |
14389 | | Similarity matrix | BLOSUM-62 |
14390 | | SS fraction | 0.3 |
14391 | | Gap open (HH/SS/other) | 18/18/6 |
14392 | | Gap extend | 1 |
14393 | | SS matrix | | | H | S | O |
14394 | | ---|---|---|--- |
14395 | | H | 6 | -9 | -6 |
14396 | | S | | 6 | -6 |
14397 | | O | | | 4 |
14398 | | Iteration cutoff | 2 |
14399 | | |
14400 | | Matchmaker beta-COPI, chain B (#66) with 20250328_COPI_GOLPH3_model_v2.cif, |
14401 | | chain J (#76), sequence alignment score = 1591 |
14402 | | RMSD between 180 pruned atom pairs is 0.463 angstroms; (across all 289 pairs: |
14403 | | 3.827) |
14404 | | |
14405 | | |
14406 | | > show #!66 models |
14407 | | |
14408 | | > hide #!66 models |
14409 | | |
14410 | | > show #!66 models |
14411 | | |
14412 | | > hide #76 models |
14413 | | |
14414 | | > show #76 models |
14415 | | |
14416 | | > hide #76 models |
14417 | | |
14418 | | > show #76 models |
14419 | | |
14420 | | > select add #76 |
14421 | | |
14422 | | 17013 atoms, 16995 bonds, 4252 residues, 1 model selected |
14423 | | |
14424 | | > select subtract #76 |
14425 | | |
14426 | | Nothing selected |
14427 | | |
14428 | | > hide #76 models |
14429 | | |
14430 | | > hide #!75 models |
14431 | | |
14432 | | > show #76 models |
14433 | | |
14434 | | > select add #76 |
14435 | | |
14436 | | 17013 atoms, 16995 bonds, 4252 residues, 1 model selected |
14437 | | |
14438 | | > ui mousemode right "translate selected models" |
14439 | | |
14440 | | > view matrix models |
14441 | | > #76,-0.94328,0.33002,0.036298,249.11,-0.21754,-0.53176,-0.81848,562.73,-0.25081,-0.77995,0.57339,257.94 |
14442 | | |
14443 | | > fitmap #76 inMap #33.1 |
14444 | | |
14445 | | Fit molecule 20250328_COPI_GOLPH3_model_v2.cif (#76) to map |
14446 | | W20_J160_run_ct6_body001.mrc (#33.1) using 17013 atoms |
14447 | | average map value = 0.005784, steps = 248 |
14448 | | shifted from previous position = 23.7 |
14449 | | rotated from previous position = 25.1 degrees |
14450 | | atoms outside contour = 13846, contour level = 0.014811 |
14451 | | |
14452 | | Position of 20250328_COPI_GOLPH3_model_v2.cif (#76) relative to |
14453 | | W20_J160_run_ct6_body001.mrc (#33.1) coordinates: |
14454 | | Matrix rotation and translation |
14455 | | -0.95309148 0.15118983 -0.26221799 379.07308450 |
14456 | | 0.09927921 -0.66223841 -0.74268696 486.62957335 |
14457 | | -0.28593754 -0.73388141 0.61616378 222.17176604 |
14458 | | Axis 0.15248160 0.41074018 -0.89891149 |
14459 | | Axis point 212.09731096 291.09961203 0.00000000 |
14460 | | Rotation angle (degrees) 178.34540300 |
14461 | | Shift along axis 57.96723338 |
14462 | | |
14463 | | |
14464 | | > select subtract #76 |
14465 | | |
14466 | | Nothing selected |
14467 | | |
14468 | | > ui tool show Matchmaker |
14469 | | |
14470 | | > matchmaker #76 to #66 |
14471 | | |
14472 | | Parameters |
14473 | | --- |
14474 | | Chain pairing | bb |
14475 | | Alignment algorithm | Needleman-Wunsch |
14476 | | Similarity matrix | BLOSUM-62 |
14477 | | SS fraction | 0.3 |
14478 | | Gap open (HH/SS/other) | 18/18/6 |
14479 | | Gap extend | 1 |
14480 | | SS matrix | | | H | S | O |
14481 | | ---|---|---|--- |
14482 | | H | 6 | -9 | -6 |
14483 | | S | | 6 | -6 |
14484 | | O | | | 4 |
14485 | | Iteration cutoff | 2 |
14486 | | |
14487 | | Matchmaker beta-COPI, chain B (#66) with 20250328_COPI_GOLPH3_model_v2.cif, |
14488 | | chain J (#76), sequence alignment score = 1591 |
14489 | | RMSD between 180 pruned atom pairs is 0.463 angstroms; (across all 289 pairs: |
14490 | | 3.827) |
14491 | | |
14492 | | |
14493 | | > undo |
14494 | | |
14495 | | > view matrix models |
14496 | | > #76,-0.94328,0.33002,0.036298,269.75,-0.21754,-0.53176,-0.81848,549.29,-0.25081,-0.77995,0.57339,234.77 |
14497 | | |
14498 | | > select subtract #76 |
14499 | | |
14500 | | Nothing selected |
14501 | | |
14502 | | > select add #76 |
14503 | | |
14504 | | 17013 atoms, 16995 bonds, 4252 residues, 1 model selected |
14505 | | |
14506 | | > view matrix models |
14507 | | > #76,-0.94328,0.33002,0.036298,244.4,-0.21754,-0.53176,-0.81848,557.07,-0.25081,-0.77995,0.57339,249.76 |
14508 | | |
14509 | | > show #!51 models |
14510 | | |
14511 | | > hide #!51 models |
14512 | | |
14513 | | > show #!52 models |
14514 | | |
14515 | | > hide #!52 models |
14516 | | |
14517 | | > hide #!66 models |
14518 | | |
14519 | | > show #!52 models |
14520 | | |
14521 | | > hide #!52 models |
14522 | | |
14523 | | > show #!51 models |
14524 | | |
14525 | | > hide #!51 models |
14526 | | |
14527 | | > ui tool show Matchmaker |
14528 | | |
14529 | | > matchmaker #76 to #51 |
14530 | | |
14531 | | Parameters |
14532 | | --- |
14533 | | Chain pairing | bb |
14534 | | Alignment algorithm | Needleman-Wunsch |
14535 | | Similarity matrix | BLOSUM-62 |
14536 | | SS fraction | 0.3 |
14537 | | Gap open (HH/SS/other) | 18/18/6 |
14538 | | Gap extend | 1 |
14539 | | SS matrix | | | H | S | O |
14540 | | ---|---|---|--- |
14541 | | H | 6 | -9 | -6 |
14542 | | S | | 6 | -6 |
14543 | | O | | | 4 |
14544 | | Iteration cutoff | 2 |
14545 | | |
14546 | | Matchmaker copy of B_fixed_model_2_B.pdb, chain B (#51) with |
14547 | | 20250328_COPI_GOLPH3_model_v2.cif, chain J (#76), sequence alignment score = |
14548 | | 1591 |
14549 | | RMSD between 180 pruned atom pairs is 0.463 angstroms; (across all 289 pairs: |
14550 | | 3.827) |
14551 | | |
14552 | | |
14553 | | > view matrix models |
14554 | | > #76,-0.94328,0.33002,0.036298,250.65,-0.21754,-0.53176,-0.81848,564.67,-0.25081,-0.77995,0.57339,248.23 |
14555 | | |
14556 | | > fitmap #76 inMap #33.1 |
14557 | | |
14558 | | Fit molecule 20250328_COPI_GOLPH3_model_v2.cif (#76) to map |
14559 | | W20_J160_run_ct6_body001.mrc (#33.1) using 17013 atoms |
14560 | | average map value = 0.005784, steps = 256 |
14561 | | shifted from previous position = 20.4 |
14562 | | rotated from previous position = 25.2 degrees |
14563 | | atoms outside contour = 13847, contour level = 0.014811 |
14564 | | |
14565 | | Position of 20250328_COPI_GOLPH3_model_v2.cif (#76) relative to |
14566 | | W20_J160_run_ct6_body001.mrc (#33.1) coordinates: |
14567 | | Matrix rotation and translation |
14568 | | -0.95306184 0.15114897 -0.26234924 379.12122640 |
14569 | | 0.09940762 -0.66223994 -0.74266842 486.60244827 |
14570 | | -0.28599171 -0.73388844 0.61613026 222.19665492 |
14571 | | Axis 0.15253453 0.41074061 -0.89890231 |
14572 | | Axis point 212.11797169 291.10158267 0.00000000 |
14573 | | Rotation angle (degrees) 178.35078282 |
14574 | | Shift along axis 57.96337940 |
14575 | | |
14576 | | |
14577 | | > select subtract #76 |
14578 | | |
14579 | | Nothing selected |
14580 | | |
14581 | | > hide #!50 models |
14582 | | |
14583 | | > show #!50 models |
14584 | | |
14585 | | > hide #!33 models |
14586 | | |
14587 | | > ui mousemode right select |
14588 | | |
14589 | | > select clear |
14590 | | |
14591 | | > select #76/F:486 |
14592 | | |
14593 | | 4 atoms, 3 bonds, 1 residue, 1 model selected |
14594 | | |
14595 | | > select up |
14596 | | |
14597 | | 24 atoms, 23 bonds, 6 residues, 1 model selected |
14598 | | |
14599 | | > select up |
14600 | | |
14601 | | 2344 atoms, 2343 bonds, 586 residues, 1 model selected |
14602 | | |
14603 | | > select up |
14604 | | |
14605 | | 17013 atoms, 16995 bonds, 4252 residues, 1 model selected |
14606 | | |
14607 | | > select down |
14608 | | |
14609 | | 2344 atoms, 2343 bonds, 586 residues, 1 model selected |
14610 | | |
14611 | | > delete sel |
14612 | | |
14613 | | > select #76/L:158 |
14614 | | |
14615 | | 4 atoms, 3 bonds, 1 residue, 1 model selected |
14616 | | |
14617 | | > select clear |
14618 | | |
14619 | | Drag select of 234 residues |
14620 | | |
14621 | | > select clear |
14622 | | |
14623 | | Drag select of 67 residues |
14624 | | |
14625 | | > select up |
14626 | | |
14627 | | 544 atoms, 538 bonds, 136 residues, 1 model selected |
14628 | | |
14629 | | > select up |
14630 | | |
14631 | | 1940 atoms, 1938 bonds, 485 residues, 1 model selected |
14632 | | |
14633 | | > delete sel |
14634 | | |
14635 | | > select #76/T:111 |
14636 | | |
14637 | | 4 atoms, 3 bonds, 1 residue, 1 model selected |
14638 | | |
14639 | | > select up |
14640 | | |
14641 | | 48 atoms, 47 bonds, 12 residues, 1 model selected |
14642 | | |
14643 | | > select up |
14644 | | |
14645 | | 669 atoms, 668 bonds, 167 residues, 1 model selected |
14646 | | |
14647 | | > delete sel |
14648 | | |
14649 | | > select clear |
14650 | | |
14651 | | [Repeated 1 time(s)] |
14652 | | |
14653 | | > select #76/L:243 |
14654 | | |
14655 | | 4 atoms, 3 bonds, 1 residue, 1 model selected |
14656 | | |
14657 | | > select up |
14658 | | |
14659 | | 48 atoms, 47 bonds, 12 residues, 1 model selected |
14660 | | |
14661 | | > select up |
14662 | | |
14663 | | 804 atoms, 803 bonds, 201 residues, 1 model selected |
14664 | | |
14665 | | > delete sel |
14666 | | |
14667 | | > select #76/K:689 |
14668 | | |
14669 | | 4 atoms, 3 bonds, 1 residue, 1 model selected |
14670 | | |
14671 | | > select up |
14672 | | |
14673 | | 40 atoms, 39 bonds, 10 residues, 1 model selected |
14674 | | |
14675 | | > select up |
14676 | | |
14677 | | 204 atoms, 203 bonds, 51 residues, 1 model selected |
14678 | | |
14679 | | > delete sel |
14680 | | |
14681 | | > select clear |
14682 | | |
14683 | | > select #76/J:133 |
14684 | | |
14685 | | 4 atoms, 3 bonds, 1 residue, 1 model selected |
14686 | | Drag select of 60 residues |
14687 | | |
14688 | | > select clear |
14689 | | |
14690 | | Drag select of 34 residues |
14691 | | |
14692 | | > select up |
14693 | | |
14694 | | 204 atoms, 200 bonds, 51 residues, 1 model selected |
14695 | | |
14696 | | > select up |
14697 | | |
14698 | | 1244 atoms, 1243 bonds, 311 residues, 1 model selected |
14699 | | |
14700 | | > delete sel |
14701 | | |
14702 | | > select clear |
14703 | | |
14704 | | > select #76/S:91 |
14705 | | |
14706 | | 4 atoms, 3 bonds, 1 residue, 1 model selected |
14707 | | |
14708 | | > select up |
14709 | | |
14710 | | 28 atoms, 27 bonds, 7 residues, 1 model selected |
14711 | | |
14712 | | > select up |
14713 | | |
14714 | | 669 atoms, 668 bonds, 167 residues, 1 model selected |
14715 | | |
14716 | | > delete sel |
14717 | | |
14718 | | > select #76/R:138 |
14719 | | |
14720 | | 4 atoms, 3 bonds, 1 residue, 1 model selected |
14721 | | |
14722 | | > select up |
14723 | | |
14724 | | 32 atoms, 31 bonds, 8 residues, 1 model selected |
14725 | | |
14726 | | > select up |
14727 | | |
14728 | | 669 atoms, 668 bonds, 167 residues, 1 model selected |
14729 | | |
14730 | | > delete sel |
14731 | | |
14732 | | > select clear |
14733 | | |
14734 | | > select #76/H:729 |
14735 | | |
14736 | | 4 atoms, 3 bonds, 1 residue, 1 model selected |
14737 | | |
14738 | | > select #76/H:727 |
14739 | | |
14740 | | 4 atoms, 3 bonds, 1 residue, 1 model selected |
14741 | | |
14742 | | > select clear |
14743 | | |
14744 | | Drag select of 12 residues |
14745 | | |
14746 | | > select clear |
14747 | | |
14748 | | Drag select of 63 residues |
14749 | | |
14750 | | > select up |
14751 | | |
14752 | | 432 atoms, 427 bonds, 108 residues, 1 model selected |
14753 | | |
14754 | | > select up |
14755 | | |
14756 | | 3337 atoms, 3335 bonds, 834 residues, 1 model selected |
14757 | | |
14758 | | > delete sel |
14759 | | |
14760 | | Drag select of 61 residues |
14761 | | |
14762 | | > select up |
14763 | | |
14764 | | 509 atoms, 503 bonds, 127 residues, 1 model selected |
14765 | | |
14766 | | > select up |
14767 | | |
14768 | | 977 atoms, 976 bonds, 244 residues, 1 model selected |
14769 | | |
14770 | | > delete sel |
14771 | | |
14772 | | > show #!33 models |
14773 | | |
14774 | | > hide #76 models |
14775 | | |
14776 | | > show #76 models |
14777 | | |
14778 | | > show #!75 models |
14779 | | |
14780 | | > hide #!75 models |
14781 | | |
14782 | | > show #!74 models |
14783 | | |
14784 | | > hide #76 models |
14785 | | |
14786 | | > show #76 models |
14787 | | |
14788 | | > hide #!74 models |
14789 | | |
14790 | | > show #!74 models |
14791 | | |
14792 | | > hide #76 models |
14793 | | |
14794 | | > hide #!50 models |
14795 | | |
14796 | | > show #76 models |
14797 | | |
14798 | | > hide #!33 models |
14799 | | |
14800 | | > select clear |
14801 | | |
14802 | | > select #76/I:400 |
14803 | | |
14804 | | 4 atoms, 3 bonds, 1 residue, 1 model selected |
14805 | | |
14806 | | > select up |
14807 | | |
14808 | | 64 atoms, 63 bonds, 16 residues, 1 model selected |
14809 | | |
14810 | | > select up |
14811 | | |
14812 | | 1252 atoms, 1251 bonds, 313 residues, 1 model selected |
14813 | | |
14814 | | > delete sel |
14815 | | |
14816 | | > show #50.9 models |
14817 | | |
14818 | | > hide #50.9 models |
14819 | | |
14820 | | > show #!50.10 models |
14821 | | |
14822 | | > hide #!50.10 models |
14823 | | |
14824 | | > show #!50.10 models |
14825 | | |
14826 | | > hide #!50.10 models |
14827 | | |
14828 | | > show #50.9 models |
14829 | | |
14830 | | > hide #50.9 models |
14831 | | |
14832 | | > show #!50.8 models |
14833 | | |
14834 | | > hide #!50.8 models |
14835 | | |
14836 | | > show #72 models |
14837 | | |
14838 | | > hide #72 models |
14839 | | |
14840 | | > show #72 models |
14841 | | |
14842 | | > hide #72 models |
14843 | | |
14844 | | > show #!75 models |
14845 | | |
14846 | | > hide #!75 models |
14847 | | |
14848 | | > hide #76 models |
14849 | | |
14850 | | > show #76 models |
14851 | | |
14852 | | > hide #!74 models |
14853 | | |
14854 | | > show #!75 models |
14855 | | |
14856 | | > hide #!75 models |
14857 | | |
14858 | | > select #76/A:21 |
14859 | | |
14860 | | 4 atoms, 3 bonds, 1 residue, 1 model selected |
14861 | | |
14862 | | > select up |
14863 | | |
14864 | | 36 atoms, 35 bonds, 9 residues, 1 model selected |
14865 | | |
14866 | | > select up |
14867 | | |
14868 | | 64 atoms, 63 bonds, 16 residues, 1 model selected |
14869 | | |
14870 | | > delete sel |
14871 | | |
14872 | | Drag select of 15 residues |
14873 | | |
14874 | | > select up |
14875 | | |
14876 | | 116 atoms, 112 bonds, 29 residues, 1 model selected |
14877 | | |
14878 | | > select up |
14879 | | |
14880 | | 580 atoms, 579 bonds, 145 residues, 1 model selected |
14881 | | |
14882 | | > delete sel |
14883 | | |
14884 | | > show #!75 models |
14885 | | |
14886 | | > hide #!75 models |
14887 | | |
14888 | | > show #!74 models |
14889 | | |
14890 | | > show #!33 models |
14891 | | |
14892 | | > select clear |
14893 | | |
14894 | | > select #76/O:107 |
14895 | | |
14896 | | 4 atoms, 3 bonds, 1 residue, 1 model selected |
14897 | | |
14898 | | > select up |
14899 | | |
14900 | | 48 atoms, 47 bonds, 12 residues, 1 model selected |
14901 | | |
14902 | | > select up |
14903 | | |
14904 | | 1132 atoms, 1131 bonds, 283 residues, 1 model selected |
14905 | | |
14906 | | > delete sel |
14907 | | |
14908 | | > hide #!33 models |
14909 | | |
14910 | | > hide #!74 models |
14911 | | |
14912 | | > show #!74 models |
14913 | | |
14914 | | > hide #76 models |
14915 | | |
14916 | | > hide #!74 models |
14917 | | |
14918 | | > show #76 models |
14919 | | |
14920 | | > select #76/M:371 |
14921 | | |
14922 | | 4 atoms, 3 bonds, 1 residue, 1 model selected |
14923 | | Drag select of 16 residues |
14924 | | |
14925 | | > select up |
14926 | | |
14927 | | 144 atoms, 142 bonds, 36 residues, 1 model selected |
14928 | | Drag select of 48 residues |
14929 | | |
14930 | | > select up |
14931 | | |
14932 | | 216 atoms, 215 bonds, 54 residues, 1 model selected |
14933 | | |
14934 | | > delete sel |
14935 | | |
14936 | | > show #!74 models |
14937 | | |
14938 | | > show #!33 models |
14939 | | |
14940 | | > show #!66 models |
14941 | | |
14942 | | > hide #76 models |
14943 | | |
14944 | | > hide #!66 models |
14945 | | |
14946 | | > show #76 models |
14947 | | |
14948 | | > hide #!33 models |
14949 | | |
14950 | | > show #!33 models |
14951 | | |
14952 | | > hide #!33 models |
14953 | | |
14954 | | > hide #76 models |
14955 | | |
14956 | | > show #!33 models |
14957 | | |
14958 | | > show #76 models |
14959 | | |
14960 | | > hide #!33 models |
14961 | | |
14962 | | > hide #!74 models |
14963 | | |
14964 | | > select #76/M:354 |
14965 | | |
14966 | | 4 atoms, 3 bonds, 1 residue, 1 model selected |
14967 | | |
14968 | | > select up |
14969 | | |
14970 | | 12 atoms, 11 bonds, 3 residues, 1 model selected |
14971 | | |
14972 | | > select up |
14973 | | |
14974 | | 380 atoms, 379 bonds, 95 residues, 1 model selected |
14975 | | |
14976 | | > select #76/M:363 |
14977 | | |
14978 | | 4 atoms, 3 bonds, 1 residue, 1 model selected |
14979 | | Drag select of 8 residues |
14980 | | |
14981 | | > select up |
14982 | | |
14983 | | 52 atoms, 51 bonds, 13 residues, 1 model selected |
14984 | | |
14985 | | > delete sel |
14986 | | |
14987 | | Drag select of 4 residues |
14988 | | |
14989 | | > select clear |
14990 | | |
14991 | | Drag select of 5 residues |
14992 | | |
14993 | | > delete sel |
14994 | | |
14995 | | > show #!74 models |
14996 | | |
14997 | | > show #!33 models |
14998 | | |
14999 | | > save "/Users/becca/Desktop/Postdoc/COPI notebooks and |
15000 | | > resources/Processing/W20_MM_cyto_COPI/20250704_W20_COPI_cyto_alignments_v2.cxs" |
15001 | | |
15002 | | [Repeated 1 time(s)] |
15003 | | |
15004 | | > save "/Users/becca/Desktop/Postdoc/COPI notebooks and |
15005 | | > resources/Processing/W20_MM_cyto_COPI/20250705_W20_COPI_cyto_alignments.cxs" |
15006 | | |
15007 | | ——— End of log from Sat Jul 5 20:47:49 2025 ——— |
15008 | | |
15009 | | > view name session-start |
15010 | | |
15011 | | opened ChimeraX session |
15012 | | |
15013 | | > open |
15014 | | > /Users/becca/Desktop/Postdoc/Phenix/W20_MM_cyto_COPI/PredictAndBuildCryoEM_7/PredictAndBuild_7_overall_best_superposed_predicted_models.pdb |
15015 | | |
15016 | | Chain information for |
15017 | | PredictAndBuild_7_overall_best_superposed_predicted_models.pdb #77 |
15018 | | --- |
15019 | | Chain | Description |
15020 | | A | No description available |
15021 | | |
15022 | | |
15023 | | > open |
15024 | | > /Users/becca/Desktop/Postdoc/Phenix/W20_MM_cyto_COPI/PredictAndBuildCryoEM_7/PredictAndBuild_7_overall_best.pdb |
15025 | | |
15026 | | Chain information for PredictAndBuild_7_overall_best.pdb #78 |
15027 | | --- |
15028 | | Chain | Description |
15029 | | A | No description available |
15030 | | |
15031 | | |
15032 | | > open |
15033 | | > /Users/becca/Desktop/Postdoc/Phenix/W20_MM_cyto_COPI/PredictAndBuildCryoEM_7/PredictAndBuild_7_superposed_predicted_untrimmed_cycle_1.pdb |
15034 | | |
15035 | | Chain information for |
15036 | | PredictAndBuild_7_superposed_predicted_untrimmed_cycle_1.pdb #79 |
15037 | | --- |
15038 | | Chain | Description |
15039 | | A | No description available |
15040 | | |
15041 | | |
15042 | | > open |
15043 | | > /Users/becca/Desktop/Postdoc/Phenix/W20_MM_cyto_COPI/PredictAndBuildCryoEM_7/PredictAndBuild_7_superposed_predicted_untrimmed_cycle_2.pdb |
15044 | | |
15045 | | Chain information for |
15046 | | PredictAndBuild_7_superposed_predicted_untrimmed_cycle_2.pdb #80 |
15047 | | --- |
15048 | | Chain | Description |
15049 | | A | No description available |
15050 | | |
15051 | | Computing secondary structure |
15052 | | [Repeated 3 time(s)] |
15053 | | |
15054 | | > ui tool show Matchmaker |
15055 | | |
15056 | | > hide #!74 models |
15057 | | |
15058 | | > show #!74 models |
15059 | | |
15060 | | > hide #!74 models |
15061 | | |
15062 | | > show #!74 models |
15063 | | |
15064 | | > matchmaker #78-80#!77 to #74 |
15065 | | |
15066 | | Parameters |
15067 | | --- |
15068 | | Chain pairing | bb |
15069 | | Alignment algorithm | Needleman-Wunsch |
15070 | | Similarity matrix | BLOSUM-62 |
15071 | | SS fraction | 0.3 |
15072 | | Gap open (HH/SS/other) | 18/18/6 |
15073 | | Gap extend | 1 |
15074 | | SS matrix | | | H | S | O |
15075 | | ---|---|---|--- |
15076 | | H | 6 | -9 | -6 |
15077 | | S | | 6 | -6 |
15078 | | O | | | 4 |
15079 | | Iteration cutoff | 2 |
15080 | | |
15081 | | Matchmaker copy of fold_20240711_copi_golph3_model_0.cif H, chain H (#74) with |
15082 | | PredictAndBuild_7_overall_best.pdb, chain A (#78), sequence alignment score = |
15083 | | 2265.6 |
15084 | | RMSD between 256 pruned atom pairs is 1.119 angstroms; (across all 355 pairs: |
15085 | | 2.263) |
15086 | | |
15087 | | Matchmaker copy of fold_20240711_copi_golph3_model_0.cif H, chain H (#74) with |
15088 | | PredictAndBuild_7_superposed_predicted_untrimmed_cycle_1.pdb, chain A (#79), |
15089 | | sequence alignment score = 2735.5 |
15090 | | RMSD between 235 pruned atom pairs is 0.805 angstroms; (across all 372 pairs: |
15091 | | 5.897) |
15092 | | |
15093 | | Matchmaker copy of fold_20240711_copi_golph3_model_0.cif H, chain H (#74) with |
15094 | | PredictAndBuild_7_superposed_predicted_untrimmed_cycle_2.pdb, chain A (#80), |
15095 | | sequence alignment score = 2729.5 |
15096 | | RMSD between 327 pruned atom pairs is 0.888 angstroms; (across all 372 pairs: |
15097 | | 4.838) |
15098 | | |
15099 | | Matchmaker copy of fold_20240711_copi_golph3_model_0.cif H, chain H (#74) with |
15100 | | PredictAndBuild_7_overall_best_superposed_predicted_models.pdb, chain A (#77), |
15101 | | sequence alignment score = 2526.8 |
15102 | | RMSD between 255 pruned atom pairs is 0.833 angstroms; (across all 351 pairs: |
15103 | | 2.256) |
15104 | | |
15105 | | |
15106 | | > hide #!74 models |
15107 | | |
15108 | | > hide #76 models |
15109 | | |
15110 | | > hide #79 models |
15111 | | |
15112 | | > hide #80 models |
15113 | | |
15114 | | > hide #78 models |
15115 | | |
15116 | | > show #76 models |
15117 | | |
15118 | | > hide #76 models |
15119 | | |
15120 | | > show #76 models |
15121 | | |
15122 | | > hide #76 models |
15123 | | |
15124 | | > show #!74 models |
15125 | | |
15126 | | > hide #!74 models |
15127 | | |
15128 | | > show #!74 models |
15129 | | |
15130 | | > hide #!33 models |
15131 | | |
15132 | | > show #!33 models |
15133 | | |
15134 | | > show #!64 models |
15135 | | |
15136 | | > show #!66 models |
15137 | | |
15138 | | > color #77 gammav3 |
15139 | | |
15140 | | > hide #!74 models |
15141 | | |
15142 | | > show #76 models |
15143 | | |
15144 | | > hide #76 models |
15145 | | |
15146 | | > show #76 models |
15147 | | |
15148 | | > hide #!77 models |
15149 | | |
15150 | | > show #!77 models |
15151 | | |
15152 | | > hide #!77 models |
15153 | | |
15154 | | > show #!77 models |
15155 | | |
15156 | | > hide #!77 models |
15157 | | |
15158 | | > show #!77 models |
15159 | | |
15160 | | > hide #!33 models |
15161 | | |
15162 | | > hide #!64 models |
15163 | | |
15164 | | > ui mousemode right select |
15165 | | |
15166 | | Drag select of 31 residues |
15167 | | |
15168 | | > select up |
15169 | | |
15170 | | 390 atoms, 396 bonds, 49 residues, 1 model selected |
15171 | | |
15172 | | > select up |
15173 | | |
15174 | | 2085 atoms, 2126 bonds, 265 residues, 1 model selected |
15175 | | |
15176 | | > select down |
15177 | | |
15178 | | 390 atoms, 396 bonds, 49 residues, 1 model selected |
15179 | | |
15180 | | > select up |
15181 | | |
15182 | | 2085 atoms, 2126 bonds, 265 residues, 1 model selected |
15183 | | |
15184 | | > show #!33 models |
15185 | | |
15186 | | > delete sel |
15187 | | |
15188 | | > hide #!33 models |
15189 | | |
15190 | | > hide #!66 models |
15191 | | |
15192 | | > show #78 models |
15193 | | |
15194 | | > hide #78 models |
15195 | | |
15196 | | > combine #77 |
15197 | | |
15198 | | [Repeated 1 time(s)] |
15199 | | |
15200 | | > hide #!77 models |
15201 | | |
15202 | | > hide #!82 models |
15203 | | |
15204 | | > hide #76 models |
15205 | | |
15206 | | > show #76 models |
15207 | | |
15208 | | > show #78 models |
15209 | | |
15210 | | > hide #!81 models |
15211 | | |
15212 | | > show #!82 models |
15213 | | |
15214 | | > hide #!82 models |
15215 | | |
15216 | | > show #80 models |
15217 | | |
15218 | | > hide #80 models |
15219 | | |
15220 | | > hide #78 models |
15221 | | |
15222 | | > show #78 models |
15223 | | |
15224 | | > hide #78 models |
15225 | | |
15226 | | > show #79 models |
15227 | | |
15228 | | > show #78 models |
15229 | | |
15230 | | > hide #78 models |
15231 | | |
15232 | | > hide #79 models |
15233 | | |
15234 | | > show #!81 models |
15235 | | |
15236 | | Drag select of 111 residues |
15237 | | |
15238 | | > select up |
15239 | | |
15240 | | 1038 atoms, 1052 bonds, 134 residues, 1 model selected |
15241 | | |
15242 | | > delete sel |
15243 | | |
15244 | | Drag select of 57 residues, 2 pseudobonds |
15245 | | |
15246 | | > select clear |
15247 | | |
15248 | | Drag select of 53 residues, 2 pseudobonds |
15249 | | |
15250 | | > delete sel |
15251 | | |
15252 | | Drag select of 69 residues, 3 pseudobonds |
15253 | | |
15254 | | > select up |
15255 | | |
15256 | | 582 atoms, 590 bonds, 3 pseudobonds, 73 residues, 2 models selected |
15257 | | |
15258 | | > delete sel |
15259 | | |
15260 | | > fitmap #81 inMap 33.3 |
15261 | | |
15262 | | Invalid "inMap" argument: invalid density map specifier |
15263 | | |
15264 | | > fitmap #81 inMap #33.3 |
15265 | | |
15266 | | Fit molecule copy of |
15267 | | PredictAndBuild_7_overall_best_superposed_predicted_models.pdb (#81) to map |
15268 | | W20_J160_run_ct6_body003.mrc (#33.3) using 2348 atoms |
15269 | | average map value = 0.0134, steps = 120 |
15270 | | shifted from previous position = 3.48 |
15271 | | rotated from previous position = 4.21 degrees |
15272 | | atoms outside contour = 1408, contour level = 0.015167 |
15273 | | |
15274 | | Position of copy of |
15275 | | PredictAndBuild_7_overall_best_superposed_predicted_models.pdb (#81) relative |
15276 | | to W20_J160_run_ct6_body003.mrc (#33.3) coordinates: |
15277 | | Matrix rotation and translation |
15278 | | -0.20129859 -0.97948015 -0.00987448 314.24082722 |
15279 | | -0.86511694 0.17304913 0.47077242 170.18293950 |
15280 | | -0.45940347 0.10330841 -0.88219942 322.78012223 |
15281 | | Axis -0.62096341 0.75964188 0.19325802 |
15282 | | Axis point 258.89532999 0.00000000 108.65698241 |
15283 | | Rotation angle (degrees) 162.78955563 |
15284 | | Shift along axis -3.47411885 |
15285 | | |
15286 | | |
15287 | | > show #!33 models |
15288 | | |
15289 | | > fitmap #81 inMap #33.1 |
15290 | | |
15291 | | Fit molecule copy of |
15292 | | PredictAndBuild_7_overall_best_superposed_predicted_models.pdb (#81) to map |
15293 | | W20_J160_run_ct6_body001.mrc (#33.1) using 2348 atoms |
15294 | | average map value = 0.01071, steps = 112 |
15295 | | shifted from previous position = 3.4 |
15296 | | rotated from previous position = 15.1 degrees |
15297 | | atoms outside contour = 1585, contour level = 0.014811 |
15298 | | |
15299 | | Position of copy of |
15300 | | PredictAndBuild_7_overall_best_superposed_predicted_models.pdb (#81) relative |
15301 | | to W20_J160_run_ct6_body001.mrc (#33.1) coordinates: |
15302 | | Matrix rotation and translation |
15303 | | -0.22739783 -0.93674263 -0.26608921 334.89658150 |
15304 | | -0.70727752 -0.02894807 0.70634306 146.59781389 |
15305 | | -0.66936442 0.34881979 -0.65595428 290.22146917 |
15306 | | Axis -0.61036394 0.68847171 0.39174298 |
15307 | | Axis point 263.55345573 -0.00000000 66.31084802 |
15308 | | Rotation angle (degrees) 162.96971650 |
15309 | | Shift along axis 10.21187265 |
15310 | | |
15311 | | |
15312 | | > hide #!33 models |
15313 | | |
15314 | | > select add #81 |
15315 | | |
15316 | | 2348 atoms, 2383 bonds, 301 residues, 1 model selected |
15317 | | Drag select of 14 residues |
15318 | | |
15319 | | > select add #81 |
15320 | | |
15321 | | 2384 atoms, 2383 bonds, 310 residues, 2 models selected |
15322 | | |
15323 | | > select subtract #81 |
15324 | | |
15325 | | 36 atoms, 9 residues, 1 model selected |
15326 | | |
15327 | | > select add #81 |
15328 | | |
15329 | | 2384 atoms, 2383 bonds, 310 residues, 2 models selected |
15330 | | |
15331 | | > ui mousemode right "translate selected models" |
15332 | | |
15333 | | > view matrix models |
15334 | | > #76,-0.93321,0.21477,-0.28809,372.34,1.5257e-05,-0.80171,-0.59771,509.2,-0.35934,-0.5578,0.74816,166.22,#81,-0.16626,-0.95754,-0.23551,330.74,-0.84489,0.015188,0.53472,191.15,-0.50844,0.28789,-0.81155,305.14 |
15335 | | |
15336 | | > view matrix models |
15337 | | > #76,-0.93321,0.21477,-0.28809,369.46,1.5257e-05,-0.80171,-0.59771,509.45,-0.35934,-0.5578,0.74816,167.85,#81,-0.16626,-0.95754,-0.23551,327.86,-0.84489,0.015188,0.53472,191.4,-0.50844,0.28789,-0.81155,306.78 |
15338 | | |
15339 | | > view matrix models |
15340 | | > #76,-0.93321,0.21477,-0.28809,362.81,1.5257e-05,-0.80171,-0.59771,510.16,-0.35934,-0.5578,0.74816,162.77,#81,-0.16626,-0.95754,-0.23551,321.21,-0.84489,0.015188,0.53472,192.11,-0.50844,0.28789,-0.81155,301.69 |
15341 | | |
15342 | | > select add #76 |
15343 | | |
15344 | | 3188 atoms, 3221 bonds, 511 residues, 2 models selected |
15345 | | |
15346 | | > show #!33 models |
15347 | | |
15348 | | > view matrix models |
15349 | | > #76,-0.93321,0.21477,-0.28809,370.55,1.5257e-05,-0.80171,-0.59771,509.34,-0.35934,-0.5578,0.74816,168.09,#81,-0.16626,-0.95754,-0.23551,328.95,-0.84489,0.015188,0.53472,191.29,-0.50844,0.28789,-0.81155,307.01 |
15350 | | |
15351 | | > select subtract #81 |
15352 | | |
15353 | | 840 atoms, 838 bonds, 210 residues, 1 model selected |
15354 | | |
15355 | | > fitmap #76 inMap #33.1 |
15356 | | |
15357 | | Fit molecule 20250328_COPI_GOLPH3_model_v2.cif (#76) to map |
15358 | | W20_J160_run_ct6_body001.mrc (#33.1) using 840 atoms |
15359 | | average map value = 0.02202, steps = 60 |
15360 | | shifted from previous position = 2.45 |
15361 | | rotated from previous position = 5.43 degrees |
15362 | | atoms outside contour = 170, contour level = 0.014811 |
15363 | | |
15364 | | Position of 20250328_COPI_GOLPH3_model_v2.cif (#76) relative to |
15365 | | W20_J160_run_ct6_body001.mrc (#33.1) coordinates: |
15366 | | Matrix rotation and translation |
15367 | | -0.96070020 0.05782811 -0.27149776 408.93422016 |
15368 | | 0.16204598 -0.67727713 -0.71766064 466.60813032 |
15369 | | -0.22538019 -0.73345184 0.64128946 199.29795302 |
15370 | | Axis -0.13724964 -0.40083218 0.90581239 |
15371 | | Axis point 212.55034942 283.76140105 0.00000000 |
15372 | | Rotation angle (degrees) 176.70210838 |
15373 | | Shift along axis -62.63107629 |
15374 | | |
15375 | | |
15376 | | > fitmap #81 inMap #33.1 |
15377 | | |
15378 | | Fit molecule copy of |
15379 | | PredictAndBuild_7_overall_best_superposed_predicted_models.pdb (#81) to map |
15380 | | W20_J160_run_ct6_body001.mrc (#33.1) using 2348 atoms |
15381 | | average map value = 0.01071, steps = 56 |
15382 | | shifted from previous position = 1.86 |
15383 | | rotated from previous position = 0.0322 degrees |
15384 | | atoms outside contour = 1584, contour level = 0.014811 |
15385 | | |
15386 | | Position of copy of |
15387 | | PredictAndBuild_7_overall_best_superposed_predicted_models.pdb (#81) relative |
15388 | | to W20_J160_run_ct6_body001.mrc (#33.1) coordinates: |
15389 | | Matrix rotation and translation |
15390 | | -0.22745563 -0.93688214 -0.26554811 334.85796779 |
15391 | | -0.70718029 -0.02854799 0.70645669 146.50571825 |
15392 | | -0.66944752 0.34847794 -0.65605118 290.29252721 |
15393 | | Axis -0.61030750 0.68859628 0.39161196 |
15394 | | Axis point 263.52285147 0.00000000 66.35287841 |
15395 | | Rotation angle (degrees) 162.94573059 |
15396 | | Shift along axis 10.19898967 |
15397 | | |
15398 | | |
15399 | | > hide #!81 models |
15400 | | |
15401 | | > hide #76 models |
15402 | | |
15403 | | > show #!81 models |
15404 | | |
15405 | | > hide #!33 models |
15406 | | |
15407 | | > view matrix models |
15408 | | > #76,-0.94628,0.12198,-0.29946,442.05,0.074145,-0.8196,-0.56813,481.51,-0.31474,-0.55981,0.76652,186.98 |
15409 | | |
15410 | | > ui mousemode right select |
15411 | | |
15412 | | Drag select of 123 residues |
15413 | | |
15414 | | > select up |
15415 | | |
15416 | | 983 atoms, 995 bonds, 129 residues, 1 model selected |
15417 | | |
15418 | | > delete sel |
15419 | | |
15420 | | > show #!33 models |
15421 | | |
15422 | | > fitmap #81 inMap #33.1 |
15423 | | |
15424 | | Fit molecule copy of |
15425 | | PredictAndBuild_7_overall_best_superposed_predicted_models.pdb (#81) to map |
15426 | | W20_J160_run_ct6_body001.mrc (#33.1) using 1365 atoms |
15427 | | average map value = 0.01415, steps = 116 |
15428 | | shifted from previous position = 2.3 |
15429 | | rotated from previous position = 16.3 degrees |
15430 | | atoms outside contour = 753, contour level = 0.014811 |
15431 | | |
15432 | | Position of copy of |
15433 | | PredictAndBuild_7_overall_best_superposed_predicted_models.pdb (#81) relative |
15434 | | to W20_J160_run_ct6_body001.mrc (#33.1) coordinates: |
15435 | | Matrix rotation and translation |
15436 | | 0.01747338 -0.99098181 -0.13285229 281.52273337 |
15437 | | -0.71306863 -0.10549261 0.69311214 162.28311421 |
15438 | | -0.70087645 0.08262179 -0.70848136 343.51081858 |
15439 | | Axis -0.69455315 0.64623949 0.31618104 |
15440 | | Axis point 0.00000000 268.41792558 210.72025848 |
15441 | | Rotation angle (degrees) 153.92902085 |
15442 | | Shift along axis 17.95286420 |
15443 | | |
15444 | | |
15445 | | > show #!74 models |
15446 | | |
15447 | | > hide #!74 models |
15448 | | |
15449 | | > show #!75 models |
15450 | | |
15451 | | > hide #!75 models |
15452 | | |
15453 | | > show #76 models |
15454 | | |
15455 | | > select add #76 |
15456 | | |
15457 | | 840 atoms, 838 bonds, 210 residues, 1 model selected |
15458 | | Drag select of 33.1 W20_J160_run_ct6_body001.mrc , 2 residues |
15459 | | |
15460 | | > select clear |
15461 | | |
15462 | | > select add #76 |
15463 | | |
15464 | | 840 atoms, 838 bonds, 210 residues, 1 model selected |
15465 | | Drag select of 33.1 W20_J160_run_ct6_body001.mrc , 5 residues |
15466 | | |
15467 | | > select add #76 |
15468 | | |
15469 | | 840 atoms, 838 bonds, 210 residues, 3 models selected |
15470 | | |
15471 | | > select subtract #76 |
15472 | | |
15473 | | 2 models selected |
15474 | | |
15475 | | > select clear |
15476 | | |
15477 | | > select add #76 |
15478 | | |
15479 | | 840 atoms, 838 bonds, 210 residues, 1 model selected |
15480 | | |
15481 | | > ui mousemode right "translate selected models" |
15482 | | |
15483 | | > view matrix models |
15484 | | > #76,-0.94628,0.12198,-0.29946,406.27,0.074145,-0.8196,-0.56813,495.91,-0.31474,-0.55981,0.76652,151.84 |
15485 | | |
15486 | | > fitmap #76 inMap #33.1 |
15487 | | |
15488 | | Fit molecule 20250328_COPI_GOLPH3_model_v2.cif (#76) to map |
15489 | | W20_J160_run_ct6_body001.mrc (#33.1) using 840 atoms |
15490 | | average map value = 0.02201, steps = 112 |
15491 | | shifted from previous position = 10.6 |
15492 | | rotated from previous position = 0.0236 degrees |
15493 | | atoms outside contour = 169, contour level = 0.014811 |
15494 | | |
15495 | | Position of 20250328_COPI_GOLPH3_model_v2.cif (#76) relative to |
15496 | | W20_J160_run_ct6_body001.mrc (#33.1) coordinates: |
15497 | | Matrix rotation and translation |
15498 | | -0.96080554 0.05790187 -0.27110898 408.84449350 |
15499 | | 0.16172293 -0.67722645 -0.71778133 466.70494589 |
15500 | | -0.22516305 -0.73349282 0.64131886 199.25734435 |
15501 | | Axis -0.13708008 -0.40087044 0.90582114 |
15502 | | Axis point 212.50907521 283.77894704 0.00000000 |
15503 | | Rotation angle (degrees) 176.71471075 |
15504 | | Shift along axis -62.64113752 |
15505 | | |
15506 | | |
15507 | | > select subtract #76 |
15508 | | |
15509 | | Nothing selected |
15510 | | |
15511 | | > hide #76 models |
15512 | | |
15513 | | > hide #!33 models |
15514 | | |
15515 | | > ui mousemode right select |
15516 | | |
15517 | | Drag select of 66 residues |
15518 | | |
15519 | | > select up |
15520 | | |
15521 | | 1365 atoms, 1387 bonds, 172 residues, 1 model selected |
15522 | | |
15523 | | > select down |
15524 | | |
15525 | | 533 atoms, 66 residues, 1 model selected |
15526 | | |
15527 | | > delete sel |
15528 | | |
15529 | | > fitmap #81 inMap #33.1 |
15530 | | |
15531 | | Fit molecule copy of |
15532 | | PredictAndBuild_7_overall_best_superposed_predicted_models.pdb (#81) to map |
15533 | | W20_J160_run_ct6_body001.mrc (#33.1) using 832 atoms |
15534 | | average map value = 0.01316, steps = 84 |
15535 | | shifted from previous position = 3.31 |
15536 | | rotated from previous position = 11.8 degrees |
15537 | | atoms outside contour = 498, contour level = 0.014811 |
15538 | | |
15539 | | Position of copy of |
15540 | | PredictAndBuild_7_overall_best_superposed_predicted_models.pdb (#81) relative |
15541 | | to W20_J160_run_ct6_body001.mrc (#33.1) coordinates: |
15542 | | Matrix rotation and translation |
15543 | | 0.14691503 -0.96385323 -0.22226771 264.04905282 |
15544 | | -0.78499351 -0.25032946 0.56667482 218.34222261 |
15545 | | -0.60183151 0.09122566 -0.79339568 335.48317698 |
15546 | | Axis -0.74977491 0.59856539 0.28205861 |
15547 | | Axis point 0.00000000 250.13903908 195.48982431 |
15548 | | Rotation angle (degrees) 161.51470465 |
15549 | | Shift along axis 27.34066185 |
15550 | | |
15551 | | |
15552 | | > show #!33 models |
15553 | | |
15554 | | > show #76 models |
15555 | | |
15556 | | > hide #!33 models |
15557 | | |
15558 | | > hide #76 models |
15559 | | |
15560 | | Drag select of 75 residues |
15561 | | |
15562 | | > delete sel |
15563 | | |
15564 | | > fitmap #81 inMap #33.1 |
15565 | | |
15566 | | Fit molecule copy of |
15567 | | PredictAndBuild_7_overall_best_superposed_predicted_models.pdb (#81) to map |
15568 | | W20_J160_run_ct6_body001.mrc (#33.1) using 239 atoms |
15569 | | average map value = 0.01661, steps = 68 |
15570 | | shifted from previous position = 0.894 |
15571 | | rotated from previous position = 22.1 degrees |
15572 | | atoms outside contour = 87, contour level = 0.014811 |
15573 | | |
15574 | | Position of copy of |
15575 | | PredictAndBuild_7_overall_best_superposed_predicted_models.pdb (#81) relative |
15576 | | to W20_J160_run_ct6_body001.mrc (#33.1) coordinates: |
15577 | | Matrix rotation and translation |
15578 | | 0.01560880 -0.84592101 -0.53307975 307.86268532 |
15579 | | -0.85667939 -0.28624403 0.42914424 255.66225987 |
15580 | | -0.51561303 0.44998001 -0.72915101 245.26898996 |
15581 | | Axis 0.71258549 -0.59736389 -0.36793789 |
15582 | | Axis point 0.00000000 255.65895561 203.35484992 |
15583 | | Rotation angle (degrees) 179.16231543 |
15584 | | Shift along axis -23.58867527 |
15585 | | |
15586 | | |
15587 | | > show #76 models |
15588 | | |
15589 | | > show #!33 models |
15590 | | |
15591 | | > hide #!33 models |
15592 | | |
15593 | | > show #!82 models |
15594 | | |
15595 | | > hide #76 models |
15596 | | |
15597 | | > hide #!81 models |
15598 | | |
15599 | | Drag select of 9 residues |
15600 | | |
15601 | | > select add #82 |
15602 | | |
15603 | | 4394 atoms, 4464 bonds, 1 pseudobond, 561 residues, 2 models selected |
15604 | | |
15605 | | > select subtract #82 |
15606 | | |
15607 | | Nothing selected |
15608 | | |
15609 | | > show #!81 models |
15610 | | |
15611 | | > hide #!82 models |
15612 | | |
15613 | | > hide #!81 models |
15614 | | |
15615 | | > show #!74 models |
15616 | | |
15617 | | > hide #!74 models |
15618 | | |
15619 | | > show #76 models |
15620 | | |
15621 | | > show #!77 models |
15622 | | |
15623 | | > hide #!77 models |
15624 | | |
15625 | | > show #78 models |
15626 | | |
15627 | | > show #!33 models |
15628 | | |
15629 | | > combine #78 |
15630 | | |
15631 | | > hide #78 models |
15632 | | |
15633 | | > hide #!33 models |
15634 | | |
15635 | | > hide #76 models |
15636 | | |
15637 | | > show #76 models |
15638 | | |
15639 | | > hide #76 models |
15640 | | |
15641 | | > show #76 models |
15642 | | |
15643 | | > hide #76 models |
15644 | | |
15645 | | Drag select of 49 residues |
15646 | | |
15647 | | > select up |
15648 | | |
15649 | | 441 atoms, 448 bonds, 56 residues, 1 model selected |
15650 | | |
15651 | | > delete sel |
15652 | | |
15653 | | > show #!74 models |
15654 | | |
15655 | | > hide #!74 models |
15656 | | |
15657 | | > show #76 models |
15658 | | |
15659 | | > fitmap #83 inMap #33.3 |
15660 | | |
15661 | | Fit molecule copy of PredictAndBuild_7_overall_best.pdb (#83) to map |
15662 | | W20_J160_run_ct6_body003.mrc (#33.3) using 3367 atoms |
15663 | | average map value = 0.01915, steps = 88 |
15664 | | shifted from previous position = 1.31 |
15665 | | rotated from previous position = 3.07 degrees |
15666 | | atoms outside contour = 1342, contour level = 0.015167 |
15667 | | |
15668 | | Position of copy of PredictAndBuild_7_overall_best.pdb (#83) relative to |
15669 | | W20_J160_run_ct6_body003.mrc (#33.3) coordinates: |
15670 | | Matrix rotation and translation |
15671 | | -0.19901990 -0.97974509 -0.02215055 315.72725675 |
15672 | | -0.86258557 0.16440368 0.47845331 170.01428108 |
15673 | | -0.46512065 0.11432847 -0.87783357 321.74102683 |
15674 | | Axis -0.62215321 0.75687028 0.20018181 |
15675 | | Axis point 259.60135583 0.00000000 107.39223084 |
15676 | | Rotation angle (degrees) 162.98435754 |
15677 | | Shift along axis -3.34526606 |
15678 | | |
15679 | | |
15680 | | > show #!33 models |
15681 | | |
15682 | | > save "/Users/becca/Desktop/Postdoc/COPI notebooks and |
15683 | | > resources/Processing/W20_MM_cyto_COPI/20250705_W20_COPI_cyto_alignments.cxs" |
15684 | | |
15685 | | > hide #!33 models |
15686 | | |
15687 | | > show #!9 models |
15688 | | |
15689 | | > hide #!9 models |
15690 | | |
15691 | | > show #!45 models |
15692 | | |
15693 | | > show #!46 models |
15694 | | |
15695 | | > hide #!46 models |
15696 | | |
15697 | | > hide #!45 models |
15698 | | |
15699 | | > show #!23 models |
15700 | | |
15701 | | > hide #!23 models |
15702 | | |
15703 | | > show #!25 models |
15704 | | |
15705 | | > fitmap #76 inMap #25 |
15706 | | |
15707 | | Fit molecule 20250328_COPI_GOLPH3_model_v2.cif (#76) to map |
15708 | | W20_J4027_005_volume_map_gammazeta_local.mrc (#25) using 840 atoms |
15709 | | average map value = 0.4915, steps = 56 |
15710 | | shifted from previous position = 1.01 |
15711 | | rotated from previous position = 4.93 degrees |
15712 | | atoms outside contour = 19, contour level = 0.2532 |
15713 | | |
15714 | | Position of 20250328_COPI_GOLPH3_model_v2.cif (#76) relative to |
15715 | | W20_J4027_005_volume_map_gammazeta_local.mrc (#25) coordinates: |
15716 | | Matrix rotation and translation |
15717 | | -0.95361160 0.05892580 -0.29521631 421.68865993 |
15718 | | 0.13475176 -0.79337176 -0.59363560 471.58547391 |
15719 | | -0.26919673 -0.60587871 0.74862815 156.24096699 |
15720 | | Axis -0.15097143 -0.32085083 0.93501999 |
15721 | | Axis point 218.58287062 267.06046758 0.00000000 |
15722 | | Rotation angle (degrees) 177.67614662 |
15723 | | Shift along axis -68.88310337 |
15724 | | |
15725 | | |
15726 | | > fitmap #77 inMap #25 |
15727 | | |
15728 | | Fit molecule PredictAndBuild_7_overall_best_superposed_predicted_models.pdb |
15729 | | (#77) to map W20_J4027_005_volume_map_gammazeta_local.mrc (#25) using 4394 |
15730 | | atoms |
15731 | | average map value = 0.2705, steps = 120 |
15732 | | shifted from previous position = 6.58 |
15733 | | rotated from previous position = 13.2 degrees |
15734 | | atoms outside contour = 2142, contour level = 0.2532 |
15735 | | |
15736 | | Position of PredictAndBuild_7_overall_best_superposed_predicted_models.pdb |
15737 | | (#77) relative to W20_J4027_005_volume_map_gammazeta_local.mrc (#25) |
15738 | | coordinates: |
15739 | | Matrix rotation and translation |
15740 | | -0.08911154 -0.97740547 -0.19167078 312.61104116 |
15741 | | -0.86513544 -0.01940313 0.50116283 207.38914458 |
15742 | | -0.49355830 0.21048058 -0.84385907 317.01597391 |
15743 | | Axis -0.66998680 0.69581356 0.25876858 |
15744 | | Axis point 280.81289084 0.00000000 94.39286792 |
15745 | | Rotation angle (degrees) 167.47114130 |
15746 | | Shift along axis 16.89268056 |
15747 | | |
15748 | | |
15749 | | > fitmap #78 inMap #25 |
15750 | | |
15751 | | Fit molecule PredictAndBuild_7_overall_best.pdb (#78) to map |
15752 | | W20_J4027_005_volume_map_gammazeta_local.mrc (#25) using 3808 atoms |
15753 | | average map value = 0.2752, steps = 92 |
15754 | | shifted from previous position = 5.3 |
15755 | | rotated from previous position = 5.46 degrees |
15756 | | atoms outside contour = 1850, contour level = 0.2532 |
15757 | | |
15758 | | Position of PredictAndBuild_7_overall_best.pdb (#78) relative to |
15759 | | W20_J4027_005_volume_map_gammazeta_local.mrc (#25) coordinates: |
15760 | | Matrix rotation and translation |
15761 | | -0.22705266 -0.96732791 -0.11279981 326.50448848 |
15762 | | -0.84045401 0.13610962 0.52451047 175.87350921 |
15763 | | -0.49202048 0.21389455 -0.84389867 316.29589561 |
15764 | | Axis -0.61344445 0.74893397 0.25056699 |
15765 | | Axis point 262.22373167 0.00000000 100.11436660 |
15766 | | Rotation angle (degrees) 165.33460884 |
15767 | | Shift along axis 10.67858762 |
15768 | | |
15769 | | |
15770 | | > fitmap #79 inMap #25 |
15771 | | |
15772 | | Fit molecule PredictAndBuild_7_superposed_predicted_untrimmed_cycle_1.pdb |
15773 | | (#79) to map W20_J4027_005_volume_map_gammazeta_local.mrc (#25) using 6818 |
15774 | | atoms |
15775 | | average map value = 0.2107, steps = 172 |
15776 | | shifted from previous position = 11.4 |
15777 | | rotated from previous position = 5.55 degrees |
15778 | | atoms outside contour = 4196, contour level = 0.2532 |
15779 | | |
15780 | | Position of PredictAndBuild_7_superposed_predicted_untrimmed_cycle_1.pdb (#79) |
15781 | | relative to W20_J4027_005_volume_map_gammazeta_local.mrc (#25) coordinates: |
15782 | | Matrix rotation and translation |
15783 | | -0.68234026 -0.66974665 -0.29300373 396.66689712 |
15784 | | -0.70556204 0.49846977 0.50369643 102.82972208 |
15785 | | -0.19129550 0.55042467 -0.81267381 199.71422066 |
15786 | | Axis 0.39762109 -0.86545829 -0.30476124 |
15787 | | Axis point 218.52168169 0.00000000 88.43752525 |
15788 | | Rotation angle (degrees) 176.63137373 |
15789 | | Shift along axis 7.86313588 |
15790 | | |
15791 | | |
15792 | | > fitmap #80 inMap #25 |
15793 | | |
15794 | | Fit molecule PredictAndBuild_7_superposed_predicted_untrimmed_cycle_2.pdb |
15795 | | (#80) to map W20_J4027_005_volume_map_gammazeta_local.mrc (#25) using 6818 |
15796 | | atoms |
15797 | | average map value = 0.2002, steps = 128 |
15798 | | shifted from previous position = 3.93 |
15799 | | rotated from previous position = 13.2 degrees |
15800 | | atoms outside contour = 4363, contour level = 0.2532 |
15801 | | |
15802 | | Position of PredictAndBuild_7_superposed_predicted_untrimmed_cycle_2.pdb (#80) |
15803 | | relative to W20_J4027_005_volume_map_gammazeta_local.mrc (#25) coordinates: |
15804 | | Matrix rotation and translation |
15805 | | -0.61808214 -0.74524170 -0.25017850 390.14177001 |
15806 | | -0.76859940 0.50608871 0.39131723 125.96407520 |
15807 | | -0.16501341 0.43415324 -0.88559671 224.77237006 |
15808 | | Axis 0.43643009 -0.86769550 -0.23797752 |
15809 | | Axis point 223.54405179 0.00000000 100.58607108 |
15810 | | Rotation angle (degrees) 177.18705406 |
15811 | | Shift along axis 7.48037378 |
15812 | | |
15813 | | |
15814 | | > fitmap #81 inMap #25 |
15815 | | |
15816 | | Fit molecule copy of |
15817 | | PredictAndBuild_7_overall_best_superposed_predicted_models.pdb (#81) to map |
15818 | | W20_J4027_005_volume_map_gammazeta_local.mrc (#25) using 239 atoms |
15819 | | average map value = 0.2847, steps = 56 |
15820 | | shifted from previous position = 2.17 |
15821 | | rotated from previous position = 9.62 degrees |
15822 | | atoms outside contour = 73, contour level = 0.2532 |
15823 | | |
15824 | | Position of copy of |
15825 | | PredictAndBuild_7_overall_best_superposed_predicted_models.pdb (#81) relative |
15826 | | to W20_J4027_005_volume_map_gammazeta_local.mrc (#25) coordinates: |
15827 | | Matrix rotation and translation |
15828 | | -0.00169329 -0.79343761 -0.60864924 319.68781006 |
15829 | | -0.93882519 -0.20835191 0.27422025 280.49121917 |
15830 | | -0.34438989 0.57187957 -0.74454641 207.08413091 |
15831 | | Axis 0.70242609 -0.62360775 -0.34309032 |
15832 | | Axis point 0.00000000 266.91836814 201.99199878 |
15833 | | Rotation angle (degrees) 167.76748361 |
15834 | | Shift along axis -21.40799989 |
15835 | | |
15836 | | |
15837 | | > fitmap #82 inMap #25 |
15838 | | |
15839 | | Fit molecule copy of |
15840 | | PredictAndBuild_7_overall_best_superposed_predicted_models.pdb (#82) to map |
15841 | | W20_J4027_005_volume_map_gammazeta_local.mrc (#25) using 4394 atoms |
15842 | | average map value = 0.2705, steps = 120 |
15843 | | shifted from previous position = 6.58 |
15844 | | rotated from previous position = 13.2 degrees |
15845 | | atoms outside contour = 2142, contour level = 0.2532 |
15846 | | |
15847 | | Position of copy of |
15848 | | PredictAndBuild_7_overall_best_superposed_predicted_models.pdb (#82) relative |
15849 | | to W20_J4027_005_volume_map_gammazeta_local.mrc (#25) coordinates: |
15850 | | Matrix rotation and translation |
15851 | | -0.08911154 -0.97740547 -0.19167078 312.61104116 |
15852 | | -0.86513544 -0.01940313 0.50116283 207.38914458 |
15853 | | -0.49355830 0.21048058 -0.84385907 317.01597391 |
15854 | | Axis -0.66998680 0.69581356 0.25876858 |
15855 | | Axis point 280.81289084 0.00000000 94.39286792 |
15856 | | Rotation angle (degrees) 167.47114130 |
15857 | | Shift along axis 16.89268056 |
15858 | | |
15859 | | |
15860 | | > fitmap #83 inMap #25 |
15861 | | |
15862 | | Fit molecule copy of PredictAndBuild_7_overall_best.pdb (#83) to map |
15863 | | W20_J4027_005_volume_map_gammazeta_local.mrc (#25) using 3367 atoms |
15864 | | average map value = 0.2736, steps = 104 |
15865 | | shifted from previous position = 4.19 |
15866 | | rotated from previous position = 7.76 degrees |
15867 | | atoms outside contour = 1660, contour level = 0.2532 |
15868 | | |
15869 | | Position of copy of PredictAndBuild_7_overall_best.pdb (#83) relative to |
15870 | | W20_J4027_005_volume_map_gammazeta_local.mrc (#25) coordinates: |
15871 | | Matrix rotation and translation |
15872 | | -0.20610544 -0.97397844 -0.09426847 320.31886481 |
15873 | | -0.84612639 0.12899838 0.51713592 179.53786545 |
15874 | | -0.49151875 0.18634757 -0.85069613 321.39018439 |
15875 | | Axis -0.62117839 0.74598543 0.24008987 |
15876 | | Axis point 263.85010261 0.00000000 102.01189567 |
15877 | | Rotation angle (degrees) 164.55823923 |
15878 | | Shift along axis 12.12000484 |
15879 | | |
15880 | | |
15881 | | > hide #83 models |
15882 | | |
15883 | | > hide #76 models |
15884 | | |
15885 | | > open |
15886 | | > /Users/becca/Desktop/Postdoc/Phenix/W20_MM_cyto_COPI/PredictAndBuildCryoEM_4/PredictAndBuild_4_overall_best_superposed_predicted_models.pdb |
15887 | | |
15888 | | Chain information for |
15889 | | PredictAndBuild_4_overall_best_superposed_predicted_models.pdb #84 |
15890 | | --- |
15891 | | Chain | Description |
15892 | | A | No description available |
15893 | | |
15894 | | |
15895 | | > open |
15896 | | > /Users/becca/Desktop/Postdoc/Phenix/W20_MM_cyto_COPI/PredictAndBuildCryoEM_4/PredictAndBuild_4_overall_best.pdb |
15897 | | |
15898 | | Chain information for PredictAndBuild_4_overall_best.pdb #85 |
15899 | | --- |
15900 | | Chain | Description |
15901 | | A | No description available |
15902 | | |
15903 | | |
15904 | | > open |
15905 | | > /Users/becca/Desktop/Postdoc/Phenix/W20_MM_cyto_COPI/PredictAndBuildCryoEM_4/PredictAndBuild_4_superposed_predicted_untrimmed_cycle_1.pdb |
15906 | | |
15907 | | Chain information for |
15908 | | PredictAndBuild_4_superposed_predicted_untrimmed_cycle_1.pdb #86 |
15909 | | --- |
15910 | | Chain | Description |
15911 | | A | No description available |
15912 | | |
15913 | | |
15914 | | > open |
15915 | | > /Users/becca/Desktop/Postdoc/Phenix/W20_MM_cyto_COPI/PredictAndBuildCryoEM_4/PredictAndBuild_4_superposed_predicted_untrimmed_cycle_2.pdb |
15916 | | |
15917 | | Chain information for |
15918 | | PredictAndBuild_4_superposed_predicted_untrimmed_cycle_2.pdb #87 |
15919 | | --- |
15920 | | Chain | Description |
15921 | | A | No description available |
15922 | | |
15923 | | |
15924 | | > open |
15925 | | > /Users/becca/Desktop/Postdoc/Phenix/W20_MM_cyto_COPI/PredictAndBuildCryoEM_4/PredictAndBuild_4_superposed_predicted_untrimmed_cycle_3.pdb |
15926 | | |
15927 | | Chain information for |
15928 | | PredictAndBuild_4_superposed_predicted_untrimmed_cycle_3.pdb #88 |
15929 | | --- |
15930 | | Chain | Description |
15931 | | A | No description available |
15932 | | |
15933 | | Computing secondary structure |
15934 | | [Repeated 4 time(s)] |
15935 | | |
15936 | | > ui tool show Matchmaker |
15937 | | |
15938 | | > matchmaker #86-88#!84-85 to #83 |
15939 | | |
15940 | | Parameters |
15941 | | --- |
15942 | | Chain pairing | bb |
15943 | | Alignment algorithm | Needleman-Wunsch |
15944 | | Similarity matrix | BLOSUM-62 |
15945 | | SS fraction | 0.3 |
15946 | | Gap open (HH/SS/other) | 18/18/6 |
15947 | | Gap extend | 1 |
15948 | | SS matrix | | | H | S | O |
15949 | | ---|---|---|--- |
15950 | | H | 6 | -9 | -6 |
15951 | | S | | 6 | -6 |
15952 | | O | | | 4 |
15953 | | Iteration cutoff | 2 |
15954 | | |
15955 | | Matchmaker copy of PredictAndBuild_7_overall_best.pdb, chain A (#83) with |
15956 | | PredictAndBuild_4_superposed_predicted_untrimmed_cycle_1.pdb, chain A (#86), |
15957 | | sequence alignment score = 2398.8 |
15958 | | RMSD between 245 pruned atom pairs is 0.859 angstroms; (across all 431 pairs: |
15959 | | 6.247) |
15960 | | |
15961 | | Matchmaker copy of PredictAndBuild_7_overall_best.pdb, chain A (#83) with |
15962 | | PredictAndBuild_4_superposed_predicted_untrimmed_cycle_2.pdb, chain A (#87), |
15963 | | sequence alignment score = 2398.8 |
15964 | | RMSD between 253 pruned atom pairs is 0.822 angstroms; (across all 431 pairs: |
15965 | | 5.398) |
15966 | | |
15967 | | Matchmaker copy of PredictAndBuild_7_overall_best.pdb, chain A (#83) with |
15968 | | PredictAndBuild_4_superposed_predicted_untrimmed_cycle_3.pdb, chain A (#88), |
15969 | | sequence alignment score = 2398.8 |
15970 | | RMSD between 255 pruned atom pairs is 0.834 angstroms; (across all 431 pairs: |
15971 | | 5.572) |
15972 | | |
15973 | | Matchmaker copy of PredictAndBuild_7_overall_best.pdb, chain A (#83) with |
15974 | | PredictAndBuild_4_overall_best_superposed_predicted_models.pdb, chain A (#84), |
15975 | | sequence alignment score = 2353 |
15976 | | RMSD between 261 pruned atom pairs is 0.933 angstroms; (across all 426 pairs: |
15977 | | 5.270) |
15978 | | |
15979 | | Matchmaker copy of PredictAndBuild_7_overall_best.pdb, chain A (#83) with |
15980 | | PredictAndBuild_4_overall_best.pdb, chain A (#85), sequence alignment score = |
15981 | | 2185.7 |
15982 | | RMSD between 274 pruned atom pairs is 0.698 angstroms; (across all 415 pairs: |
15983 | | 4.042) |
15984 | | |
15985 | | |
15986 | | > open "/Users/becca/Desktop/Postdoc/COPI notebooks and resources/Alphafold |
15987 | | > predictions/fold_20250704_copi_abbprimedgz_trucadg/fold_20250704_copi_abbprimedgz_trucadg_model_0.cif" |
15988 | | |
15989 | | Chain information for fold_20250704_copi_abbprimedgz_trucadg_model_0.cif #89 |
15990 | | --- |
15991 | | Chain | Description |
15992 | | A | . |
15993 | | B | . |
15994 | | C | . |
15995 | | D | . |
15996 | | E | . |
15997 | | F | . |
15998 | | |
15999 | | |
16000 | | > open "/Users/becca/Desktop/Postdoc/COPI notebooks and resources/Alphafold |
16001 | | > predictions/fold_20250704_copi_abbprimedgz_trucadg/fold_20250704_copi_abbprimedgz_trucadg_model_1.cif" |
16002 | | |
16003 | | Chain information for fold_20250704_copi_abbprimedgz_trucadg_model_1.cif #90 |
16004 | | --- |
16005 | | Chain | Description |
16006 | | A | . |
16007 | | B | . |
16008 | | C | . |
16009 | | D | . |
16010 | | E | . |
16011 | | F | . |
16012 | | |
16013 | | |
16014 | | > open "/Users/becca/Desktop/Postdoc/COPI notebooks and resources/Alphafold |
16015 | | > predictions/fold_20250704_copi_abbprimedgz_trucadg/fold_20250704_copi_abbprimedgz_trucadg_model_2.cif" |
16016 | | |
16017 | | Chain information for fold_20250704_copi_abbprimedgz_trucadg_model_2.cif #91 |
16018 | | --- |
16019 | | Chain | Description |
16020 | | A | . |
16021 | | B | . |
16022 | | C | . |
16023 | | D | . |
16024 | | E | . |
16025 | | F | . |
16026 | | |
16027 | | |
16028 | | > open "/Users/becca/Desktop/Postdoc/COPI notebooks and resources/Alphafold |
16029 | | > predictions/fold_20250704_copi_abbprimedgz_trucadg/fold_20250704_copi_abbprimedgz_trucadg_model_3.cif" |
16030 | | |
16031 | | Chain information for fold_20250704_copi_abbprimedgz_trucadg_model_3.cif #92 |
16032 | | --- |
16033 | | Chain | Description |
16034 | | A | . |
16035 | | B | . |
16036 | | C | . |
16037 | | D | . |
16038 | | E | . |
16039 | | F | . |
16040 | | |
16041 | | |
16042 | | > open "/Users/becca/Desktop/Postdoc/COPI notebooks and resources/Alphafold |
16043 | | > predictions/fold_20250704_copi_abbprimedgz_trucadg/fold_20250704_copi_abbprimedgz_trucadg_model_4.cif" |
16044 | | |
16045 | | Chain information for fold_20250704_copi_abbprimedgz_trucadg_model_4.cif #93 |
16046 | | --- |
16047 | | Chain | Description |
16048 | | A | . |
16049 | | B | . |
16050 | | C | . |
16051 | | D | . |
16052 | | E | . |
16053 | | F | . |
16054 | | |
16055 | | |
16056 | | > open "/Users/becca/Desktop/Postdoc/COPI notebooks and resources/Structure |
16057 | | > files/COPI_soluble_job160_dhelix.pdb" |
16058 | | |
16059 | | Chain information for COPI_soluble_job160_dhelix.pdb #94 |
16060 | | --- |
16061 | | Chain | Description |
16062 | | D | No description available |
16063 | | E | No description available |
16064 | | F | No description available |
16065 | | G | No description available |
16066 | | H | No description available |
16067 | | I | No description available |
16068 | | J | No description available |
16069 | | K | No description available |
16070 | | L | No description available |
16071 | | M | No description available |
16072 | | N | No description available |
16073 | | O | No description available |
16074 | | P | No description available |
16075 | | Q | No description available |
16076 | | |
16077 | | |
16078 | | > open "/Users/becca/Desktop/Postdoc/COPI notebooks and resources/Structure |
16079 | | > files/COPI_soluble_job155_dhelix.pdb" |
16080 | | |
16081 | | Chain information for COPI_soluble_job155_dhelix.pdb #95 |
16082 | | --- |
16083 | | Chain | Description |
16084 | | D | No description available |
16085 | | E | No description available |
16086 | | F | No description available |
16087 | | G | No description available |
16088 | | H | No description available |
16089 | | I | No description available |
16090 | | J | No description available |
16091 | | K | No description available |
16092 | | L | No description available |
16093 | | M | No description available |
16094 | | N | No description available |
16095 | | O | No description available |
16096 | | P | No description available |
16097 | | Q | No description available |
16098 | | |
16099 | | |
16100 | | > open "/Users/becca/Desktop/Postdoc/COPI notebooks and resources/Structure |
16101 | | > files/COPI_soluble_job140_dhelix.pdb" |
16102 | | |
16103 | | Chain information for COPI_soluble_job140_dhelix.pdb #96 |
16104 | | --- |
16105 | | Chain | Description |
16106 | | D | No description available |
16107 | | E | No description available |
16108 | | F | No description available |
16109 | | G | No description available |
16110 | | H | No description available |
16111 | | I | No description available |
16112 | | J | No description available |
16113 | | K | No description available |
16114 | | L | No description available |
16115 | | M | No description available |
16116 | | N | No description available |
16117 | | O | No description available |
16118 | | P | No description available |
16119 | | Q | No description available |
16120 | | |
16121 | | |
16122 | | > open "/Users/becca/Desktop/Postdoc/COPI notebooks and resources/Structure |
16123 | | > files/COPI_soluble_job150_dhelix.pdb" |
16124 | | |
16125 | | Chain information for COPI_soluble_job150_dhelix.pdb #97 |
16126 | | --- |
16127 | | Chain | Description |
16128 | | D | No description available |
16129 | | E | No description available |
16130 | | F | No description available |
16131 | | G | No description available |
16132 | | H | No description available |
16133 | | I | No description available |
16134 | | J | No description available |
16135 | | K | No description available |
16136 | | L | No description available |
16137 | | M | No description available |
16138 | | N | No description available |
16139 | | O | No description available |
16140 | | P | No description available |
16141 | | Q | No description available |
16142 | | |
16143 | | |
16144 | | > open "/Users/becca/Desktop/Postdoc/COPI notebooks and resources/Structure |
16145 | | > files/COPI_soluble_job145_dhelix.pdb" |
16146 | | |
16147 | | Chain information for COPI_soluble_job145_dhelix.pdb #98 |
16148 | | --- |
16149 | | Chain | Description |
16150 | | D | No description available |
16151 | | E | No description available |
16152 | | F | No description available |
16153 | | G | No description available |
16154 | | H | No description available |
16155 | | I | No description available |
16156 | | J | No description available |
16157 | | K | No description available |
16158 | | L | No description available |
16159 | | M | No description available |
16160 | | N | No description available |
16161 | | O | No description available |
16162 | | P | No description available |
16163 | | Q | No description available |
16164 | | |
16165 | | |
16166 | | > ui tool show Matchmaker |
16167 | | |
16168 | | > matchmaker #89-93#!94-98 to #83 |
16169 | | |
16170 | | Computing secondary structure |
16171 | | [Repeated 4 time(s)] Parameters |
16172 | | --- |
16173 | | Chain pairing | bb |
16174 | | Alignment algorithm | Needleman-Wunsch |
16175 | | Similarity matrix | BLOSUM-62 |
16176 | | SS fraction | 0.3 |
16177 | | Gap open (HH/SS/other) | 18/18/6 |
16178 | | Gap extend | 1 |
16179 | | SS matrix | | | H | S | O |
16180 | | ---|---|---|--- |
16181 | | H | 6 | -9 | -6 |
16182 | | S | | 6 | -6 |
16183 | | O | | | 4 |
16184 | | Iteration cutoff | 2 |
16185 | | |
16186 | | Matchmaker copy of PredictAndBuild_7_overall_best.pdb, chain A (#83) with |
16187 | | fold_20250704_copi_abbprimedgz_trucadg_model_0.cif, chain E (#89), sequence |
16188 | | alignment score = 2394.6 |
16189 | | RMSD between 254 pruned atom pairs is 1.108 angstroms; (across all 431 pairs: |
16190 | | 2.715) |
16191 | | |
16192 | | Matchmaker copy of PredictAndBuild_7_overall_best.pdb, chain A (#83) with |
16193 | | fold_20250704_copi_abbprimedgz_trucadg_model_1.cif, chain E (#90), sequence |
16194 | | alignment score = 2394.6 |
16195 | | RMSD between 252 pruned atom pairs is 1.085 angstroms; (across all 431 pairs: |
16196 | | 2.724) |
16197 | | |
16198 | | Matchmaker copy of PredictAndBuild_7_overall_best.pdb, chain A (#83) with |
16199 | | fold_20250704_copi_abbprimedgz_trucadg_model_2.cif, chain E (#91), sequence |
16200 | | alignment score = 2391 |
16201 | | RMSD between 245 pruned atom pairs is 1.089 angstroms; (across all 431 pairs: |
16202 | | 2.930) |
16203 | | |
16204 | | Matchmaker copy of PredictAndBuild_7_overall_best.pdb, chain A (#83) with |
16205 | | fold_20250704_copi_abbprimedgz_trucadg_model_3.cif, chain E (#92), sequence |
16206 | | alignment score = 2401.8 |
16207 | | RMSD between 243 pruned atom pairs is 1.065 angstroms; (across all 431 pairs: |
16208 | | 3.641) |
16209 | | |
16210 | | Matchmaker copy of PredictAndBuild_7_overall_best.pdb, chain A (#83) with |
16211 | | fold_20250704_copi_abbprimedgz_trucadg_model_4.cif, chain E (#93), sequence |
16212 | | alignment score = 2401.8 |
16213 | | RMSD between 241 pruned atom pairs is 1.095 angstroms; (across all 431 pairs: |
16214 | | 2.725) |
16215 | | |
16216 | | Matchmaker copy of PredictAndBuild_7_overall_best.pdb, chain A (#83) with |
16217 | | COPI_soluble_job160_dhelix.pdb, chain M (#94), sequence alignment score = |
16218 | | 1452.2 |
16219 | | RMSD between 239 pruned atom pairs is 1.088 angstroms; (across all 282 pairs: |
16220 | | 1.700) |
16221 | | |
16222 | | Matchmaker copy of PredictAndBuild_7_overall_best.pdb, chain A (#83) with |
16223 | | COPI_soluble_job155_dhelix.pdb, chain M (#95), sequence alignment score = |
16224 | | 1452.2 |
16225 | | RMSD between 239 pruned atom pairs is 1.088 angstroms; (across all 282 pairs: |
16226 | | 1.700) |
16227 | | |
16228 | | Matchmaker copy of PredictAndBuild_7_overall_best.pdb, chain A (#83) with |
16229 | | COPI_soluble_job140_dhelix.pdb, chain M (#96), sequence alignment score = |
16230 | | 1452.2 |
16231 | | RMSD between 239 pruned atom pairs is 1.088 angstroms; (across all 282 pairs: |
16232 | | 1.700) |
16233 | | |
16234 | | Matchmaker copy of PredictAndBuild_7_overall_best.pdb, chain A (#83) with |
16235 | | COPI_soluble_job150_dhelix.pdb, chain M (#97), sequence alignment score = |
16236 | | 1452.2 |
16237 | | RMSD between 239 pruned atom pairs is 1.088 angstroms; (across all 282 pairs: |
16238 | | 1.700) |
16239 | | |
16240 | | Matchmaker copy of PredictAndBuild_7_overall_best.pdb, chain A (#83) with |
16241 | | COPI_soluble_job145_dhelix.pdb, chain M (#98), sequence alignment score = |
16242 | | 1452.2 |
16243 | | RMSD between 239 pruned atom pairs is 1.088 angstroms; (across all 282 pairs: |
16244 | | 1.700) |
16245 | | |
16246 | | |
16247 | | > hide #86-93#!84-85,94-98 atoms |
16248 | | |
16249 | | > show #86-93#!84-85,94-98 cartoons |
16250 | | |
16251 | | > hide #93 models |
16252 | | |
16253 | | > hide #92 models |
16254 | | |
16255 | | > hide #91 models |
16256 | | |
16257 | | > hide #90 models |
16258 | | |
16259 | | > hide #89 models |
16260 | | |
16261 | | > hide #88 models |
16262 | | |
16263 | | > hide #87 models |
16264 | | |
16265 | | > hide #86 models |
16266 | | |
16267 | | > hide #!85 models |
16268 | | |
16269 | | > hide #!84 models |
16270 | | |
16271 | | > hide #!98 models |
16272 | | |
16273 | | > hide #!97 models |
16274 | | |
16275 | | > hide #!96 models |
16276 | | |
16277 | | > hide #!95 models |
16278 | | |
16279 | | > hide #!94 models |
16280 | | |
16281 | | > show #89 models |
16282 | | |
16283 | | > fitmap #89 inMap #25 |
16284 | | |
16285 | | Fit molecule fold_20250704_copi_abbprimedgz_trucadg_model_0.cif (#89) to map |
16286 | | W20_J4027_005_volume_map_gammazeta_local.mrc (#25) using 31307 atoms |
16287 | | average map value = 0.1729, steps = 144 |
16288 | | shifted from previous position = 3.16 |
16289 | | rotated from previous position = 14.3 degrees |
16290 | | atoms outside contour = 21652, contour level = 0.2532 |
16291 | | |
16292 | | Position of fold_20250704_copi_abbprimedgz_trucadg_model_0.cif (#89) relative |
16293 | | to W20_J4027_005_volume_map_gammazeta_local.mrc (#25) coordinates: |
16294 | | Matrix rotation and translation |
16295 | | 0.05328171 -0.76106194 -0.64648727 140.92255080 |
16296 | | 0.28101753 0.63267016 -0.72163538 147.85576557 |
16297 | | 0.95822242 -0.14322428 0.24758149 143.21536141 |
16298 | | Axis 0.28936539 -0.80279829 0.52132770 |
16299 | | Axis point 9.80405567 0.00000000 205.08316869 |
16300 | | Rotation angle (degrees) 91.90447951 |
16301 | | Shift along axis -3.25811224 |
16302 | | |
16303 | | |
16304 | | > hide #89 models |
16305 | | |
16306 | | > show #!94 models |
16307 | | |
16308 | | > fitmap #94 inMap #25 |
16309 | | |
16310 | | Fit molecule COPI_soluble_job160_dhelix.pdb (#94) to map |
16311 | | W20_J4027_005_volume_map_gammazeta_local.mrc (#25) using 27657 atoms |
16312 | | average map value = 0.2718, steps = 204 |
16313 | | shifted from previous position = 15.7 |
16314 | | rotated from previous position = 16.6 degrees |
16315 | | atoms outside contour = 14003, contour level = 0.2532 |
16316 | | |
16317 | | Position of COPI_soluble_job160_dhelix.pdb (#94) relative to |
16318 | | W20_J4027_005_volume_map_gammazeta_local.mrc (#25) coordinates: |
16319 | | Matrix rotation and translation |
16320 | | -0.94072620 0.21431989 -0.26287109 429.26780955 |
16321 | | 0.04392373 -0.69153884 -0.72100259 611.48757588 |
16322 | | -0.33631076 -0.68981230 0.64113497 251.13731297 |
16323 | | Axis 0.16577200 0.39032157 -0.90563167 |
16324 | | Axis point 252.69703805 349.04589066 0.00000000 |
16325 | | Rotation angle (degrees) 174.60186768 |
16326 | | Shift along axis 82.39947160 |
16327 | | |
16328 | | |
16329 | | > fitmap #95 inMap #25 |
16330 | | |
16331 | | Fit molecule COPI_soluble_job155_dhelix.pdb (#95) to map |
16332 | | W20_J4027_005_volume_map_gammazeta_local.mrc (#25) using 27657 atoms |
16333 | | average map value = 0.2423, steps = 164 |
16334 | | shifted from previous position = 15.2 |
16335 | | rotated from previous position = 11.8 degrees |
16336 | | atoms outside contour = 15830, contour level = 0.2532 |
16337 | | |
16338 | | Position of COPI_soluble_job155_dhelix.pdb (#95) relative to |
16339 | | W20_J4027_005_volume_map_gammazeta_local.mrc (#25) coordinates: |
16340 | | Matrix rotation and translation |
16341 | | -0.93495608 0.23558445 -0.26524914 421.51639111 |
16342 | | 0.02686846 -0.69850918 -0.71509651 616.29442709 |
16343 | | -0.35374457 -0.67571066 0.64674561 248.96083246 |
16344 | | Axis 0.17116994 0.38459904 -0.90707466 |
16345 | | Axis point 252.83551002 347.97043032 0.00000000 |
16346 | | Rotation angle (degrees) 173.39355126 |
16347 | | Shift along axis 83.35111726 |
16348 | | |
16349 | | |
16350 | | > fitmap #96 inMap #25 |
16351 | | |
16352 | | Fit molecule COPI_soluble_job140_dhelix.pdb (#96) to map |
16353 | | W20_J4027_005_volume_map_gammazeta_local.mrc (#25) using 27657 atoms |
16354 | | average map value = 0.2622, steps = 256 |
16355 | | shifted from previous position = 18.3 |
16356 | | rotated from previous position = 12.3 degrees |
16357 | | atoms outside contour = 14721, contour level = 0.2532 |
16358 | | |
16359 | | Position of COPI_soluble_job140_dhelix.pdb (#96) relative to |
16360 | | W20_J4027_005_volume_map_gammazeta_local.mrc (#25) coordinates: |
16361 | | Matrix rotation and translation |
16362 | | -0.93956714 0.21928330 -0.26292285 427.34338098 |
16363 | | 0.03882046 -0.69477206 -0.71818156 612.97301969 |
16364 | | -0.34015668 -0.68498658 0.64427232 249.37443237 |
16365 | | Axis 0.16673989 0.38794901 -0.90647304 |
16366 | | Axis point 252.59063007 348.46957852 0.00000000 |
16367 | | Rotation angle (degrees) 174.28724830 |
16368 | | Shift along axis 83.00626481 |
16369 | | |
16370 | | |
16371 | | > fitmap #97 inMap #25 |
16372 | | |
16373 | | Fit molecule COPI_soluble_job150_dhelix.pdb (#97) to map |
16374 | | W20_J4027_005_volume_map_gammazeta_local.mrc (#25) using 27657 atoms |
16375 | | average map value = 0.2628, steps = 212 |
16376 | | shifted from previous position = 21 |
16377 | | rotated from previous position = 15.7 degrees |
16378 | | atoms outside contour = 14726, contour level = 0.2532 |
16379 | | |
16380 | | Position of COPI_soluble_job150_dhelix.pdb (#97) relative to |
16381 | | W20_J4027_005_volume_map_gammazeta_local.mrc (#25) coordinates: |
16382 | | Matrix rotation and translation |
16383 | | -0.94025104 0.21735184 -0.26208044 427.88998347 |
16384 | | 0.04057084 -0.69272872 -0.72005619 612.48408539 |
16385 | | -0.33805619 -0.68766641 0.64252076 250.33040167 |
16386 | | Axis 0.16599686 0.38937389 -0.90599836 |
16387 | | Axis point 252.53671335 348.85846385 0.00000000 |
16388 | | Rotation angle (degrees) 174.40123581 |
16389 | | Shift along axis 82.71476836 |
16390 | | |
16391 | | |
16392 | | > fitmap #98 inMap #25 |
16393 | | |
16394 | | Fit molecule COPI_soluble_job145_dhelix.pdb (#98) to map |
16395 | | W20_J4027_005_volume_map_gammazeta_local.mrc (#25) using 27657 atoms |
16396 | | average map value = 0.2587, steps = 272 |
16397 | | shifted from previous position = 18 |
16398 | | rotated from previous position = 11.9 degrees |
16399 | | atoms outside contour = 15067, contour level = 0.2532 |
16400 | | |
16401 | | Position of COPI_soluble_job145_dhelix.pdb (#98) relative to |
16402 | | W20_J4027_005_volume_map_gammazeta_local.mrc (#25) coordinates: |
16403 | | Matrix rotation and translation |
16404 | | -0.93993457 0.21619613 -0.26416328 429.04174114 |
16405 | | 0.04215368 -0.69443036 -0.71832412 612.05358105 |
16406 | | -0.33874190 -0.68631312 0.64360564 249.64587097 |
16407 | | Axis 0.16669352 0.38835946 -0.90630580 |
16408 | | Axis point 252.87215354 348.49166546 0.00000000 |
16409 | | Rotation angle (degrees) 174.49011360 |
16410 | | Shift along axis 82.95977526 |
16411 | | |
16412 | | |
16413 | | > hide #!94 models |
16414 | | |
16415 | | > show #!94 models |
16416 | | |
16417 | | > hide #!94 models |
16418 | | |
16419 | | > show #!95 models |
16420 | | |
16421 | | > hide #!95 models |
16422 | | |
16423 | | > show #!94 models |
16424 | | |
16425 | | > hide #!50 models |
16426 | | |
16427 | | > show #!33 models |
16428 | | |
16429 | | > hide #!25 models |
16430 | | |
16431 | | > hide #!33 models |
16432 | | |
16433 | | > show #!25 models |
16434 | | |
16435 | | > close #1 |
16436 | | |
16437 | | > close #4 |
16438 | | |
16439 | | > close #6 |
16440 | | |
16441 | | > close #8 |
16442 | | |
16443 | | > close #10 |
16444 | | |
16445 | | > close #12 |
16446 | | |
16447 | | > close #14 |
16448 | | |
16449 | | > close #16 |
16450 | | |
16451 | | > close #18 |
16452 | | |
16453 | | > close #20 |
16454 | | |
16455 | | > close #22 |
16456 | | |
16457 | | > close #24 |
16458 | | |
16459 | | > close #26 |
16460 | | |
16461 | | > close #28 |
16462 | | |
16463 | | > close #41 |
16464 | | |
16465 | | > hide #!25 models |
16466 | | |
16467 | | > hide #!94 models |
16468 | | |
16469 | | > show #!96 models |
16470 | | |
16471 | | > show #!95 models |
16472 | | |
16473 | | > hide #!95 models |
16474 | | |
16475 | | > show #!94 models |
16476 | | |
16477 | | > hide #!94 models |
16478 | | |
16479 | | > show #!97 models |
16480 | | |
16481 | | > hide #!97 models |
16482 | | |
16483 | | > show #!98 models |
16484 | | |
16485 | | > hide #!98 models |
16486 | | |
16487 | | > hide #!96 models |
16488 | | |
16489 | | > show #!94 models |
16490 | | |
16491 | | > split #94 chains |
16492 | | |
16493 | | Split COPI_soluble_job160_dhelix.pdb (#94) into 14 models |
16494 | | Chain information for COPI_soluble_job160_dhelix.pdb D #94.1 |
16495 | | --- |
16496 | | Chain | Description |
16497 | | D | No description available |
16498 | | |
16499 | | Chain information for COPI_soluble_job160_dhelix.pdb E #94.2 |
16500 | | --- |
16501 | | Chain | Description |
16502 | | E | No description available |
16503 | | |
16504 | | Chain information for COPI_soluble_job160_dhelix.pdb F #94.3 |
16505 | | --- |
16506 | | Chain | Description |
16507 | | F | No description available |
16508 | | |
16509 | | Chain information for COPI_soluble_job160_dhelix.pdb G #94.4 |
16510 | | --- |
16511 | | Chain | Description |
16512 | | G | No description available |
16513 | | |
16514 | | Chain information for COPI_soluble_job160_dhelix.pdb H #94.5 |
16515 | | --- |
16516 | | Chain | Description |
16517 | | H | No description available |
16518 | | |
16519 | | Chain information for COPI_soluble_job160_dhelix.pdb I #94.6 |
16520 | | --- |
16521 | | Chain | Description |
16522 | | I | No description available |
16523 | | |
16524 | | Chain information for COPI_soluble_job160_dhelix.pdb J #94.7 |
16525 | | --- |
16526 | | Chain | Description |
16527 | | J | No description available |
16528 | | |
16529 | | Chain information for COPI_soluble_job160_dhelix.pdb K #94.8 |
16530 | | --- |
16531 | | Chain | Description |
16532 | | K | No description available |
16533 | | |
16534 | | Chain information for COPI_soluble_job160_dhelix.pdb L #94.9 |
16535 | | --- |
16536 | | Chain | Description |
16537 | | L | No description available |
16538 | | |
16539 | | Chain information for COPI_soluble_job160_dhelix.pdb M #94.10 |
16540 | | --- |
16541 | | Chain | Description |
16542 | | M | No description available |
16543 | | |
16544 | | Chain information for COPI_soluble_job160_dhelix.pdb N #94.11 |
16545 | | --- |
16546 | | Chain | Description |
16547 | | N | No description available |
16548 | | |
16549 | | Chain information for COPI_soluble_job160_dhelix.pdb O #94.12 |
16550 | | --- |
16551 | | Chain | Description |
16552 | | O | No description available |
16553 | | |
16554 | | Chain information for COPI_soluble_job160_dhelix.pdb P #94.13 |
16555 | | --- |
16556 | | Chain | Description |
16557 | | P | No description available |
16558 | | |
16559 | | Chain information for COPI_soluble_job160_dhelix.pdb Q #94.14 |
16560 | | --- |
16561 | | Chain | Description |
16562 | | Q | No description available |
16563 | | |
16564 | | |
16565 | | > show #!95 models |
16566 | | |
16567 | | > hide #!95 models |
16568 | | |
16569 | | > close #95 |
16570 | | |
16571 | | > close #96 |
16572 | | |
16573 | | > close #97 |
16574 | | |
16575 | | > close #98 |
16576 | | |
16577 | | > hide #94.1 models |
16578 | | |
16579 | | > hide #94.2 models |
16580 | | |
16581 | | > hide #94.3 models |
16582 | | |
16583 | | > hide #94.4 models |
16584 | | |
16585 | | > hide #94.5 models |
16586 | | |
16587 | | > hide #!94.6 models |
16588 | | |
16589 | | > hide #!94.7 models |
16590 | | |
16591 | | > hide #94.8 models |
16592 | | |
16593 | | > show #94.8 models |
16594 | | |
16595 | | > hide #94.8 models |
16596 | | |
16597 | | > hide #94.12 models |
16598 | | |
16599 | | > hide #!94.13 models |
16600 | | |
16601 | | > hide #!94.14 models |
16602 | | |
16603 | | > show #89 models |
16604 | | |
16605 | | > show #!25 models |
16606 | | |
16607 | | > hide #89 models |
16608 | | |
16609 | | > show #90 models |
16610 | | |
16611 | | > show #89 models |
16612 | | |
16613 | | > hide #89 models |
16614 | | |
16615 | | > hide #90 models |
16616 | | |
16617 | | > show #91 models |
16618 | | |
16619 | | > hide #91 models |
16620 | | |
16621 | | > show #92 models |
16622 | | |
16623 | | > hide #92 models |
16624 | | |
16625 | | > show #93 models |
16626 | | |
16627 | | > hide #93 models |
16628 | | |
16629 | | > show #88 models |
16630 | | |
16631 | | > hide #88 models |
16632 | | |
16633 | | > show #78 models |
16634 | | |
16635 | | > hide #78 models |
16636 | | |
16637 | | > show #!81 models |
16638 | | |
16639 | | > hide #!81 models |
16640 | | |
16641 | | > show #!81 models |
16642 | | |
16643 | | > hide #!81 models |
16644 | | |
16645 | | > show #!82 models |
16646 | | |
16647 | | > hide #!82 models |
16648 | | |
16649 | | > show #79 models |
16650 | | |
16651 | | > hide #79 models |
16652 | | |
16653 | | > show #80 models |
16654 | | |
16655 | | > fitmap #80 inMap #25 |
16656 | | |
16657 | | Fit molecule PredictAndBuild_7_superposed_predicted_untrimmed_cycle_2.pdb |
16658 | | (#80) to map W20_J4027_005_volume_map_gammazeta_local.mrc (#25) using 6818 |
16659 | | atoms |
16660 | | average map value = 0.2002, steps = 28 |
16661 | | shifted from previous position = 0.0204 |
16662 | | rotated from previous position = 0.023 degrees |
16663 | | atoms outside contour = 4363, contour level = 0.2532 |
16664 | | |
16665 | | Position of PredictAndBuild_7_superposed_predicted_untrimmed_cycle_2.pdb (#80) |
16666 | | relative to W20_J4027_005_volume_map_gammazeta_local.mrc (#25) coordinates: |
16667 | | Matrix rotation and translation |
16668 | | -0.61827994 -0.74501686 -0.25035934 390.17808029 |
16669 | | -0.76844646 0.50614879 0.39153982 125.89539509 |
16670 | | -0.16498469 0.43446897 -0.88544721 224.70859005 |
16671 | | Axis 0.43631396 -0.86771240 -0.23812878 |
16672 | | Axis point 223.52898003 0.00000000 100.56670382 |
16673 | | Rotation angle (degrees) 177.18018194 |
16674 | | Shift along axis 7.48956636 |
16675 | | |
16676 | | |
16677 | | > ui tool show Matchmaker |
16678 | | |
16679 | | > matchmaker #80 to #94.9 |
16680 | | |
16681 | | Parameters |
16682 | | --- |
16683 | | Chain pairing | bb |
16684 | | Alignment algorithm | Needleman-Wunsch |
16685 | | Similarity matrix | BLOSUM-62 |
16686 | | SS fraction | 0.3 |
16687 | | Gap open (HH/SS/other) | 18/18/6 |
16688 | | Gap extend | 1 |
16689 | | SS matrix | | | H | S | O |
16690 | | ---|---|---|--- |
16691 | | H | 6 | -9 | -6 |
16692 | | S | | 6 | -6 |
16693 | | O | | | 4 |
16694 | | Iteration cutoff | 2 |
16695 | | |
16696 | | Matchmaker COPI_soluble_job160_dhelix.pdb L, chain L (#94.9) with |
16697 | | PredictAndBuild_7_superposed_predicted_untrimmed_cycle_2.pdb, chain A (#80), |
16698 | | sequence alignment score = 751 |
16699 | | RMSD between 111 pruned atom pairs is 0.761 angstroms; (across all 149 pairs: |
16700 | | 2.111) |
16701 | | |
16702 | | |
16703 | | > ui tool show Matchmaker |
16704 | | |
16705 | | > matchmaker #80 to #94.10 |
16706 | | |
16707 | | Parameters |
16708 | | --- |
16709 | | Chain pairing | bb |
16710 | | Alignment algorithm | Needleman-Wunsch |
16711 | | Similarity matrix | BLOSUM-62 |
16712 | | SS fraction | 0.3 |
16713 | | Gap open (HH/SS/other) | 18/18/6 |
16714 | | Gap extend | 1 |
16715 | | SS matrix | | | H | S | O |
16716 | | ---|---|---|--- |
16717 | | H | 6 | -9 | -6 |
16718 | | S | | 6 | -6 |
16719 | | O | | | 4 |
16720 | | Iteration cutoff | 2 |
16721 | | |
16722 | | Matchmaker COPI_soluble_job160_dhelix.pdb M, chain M (#94.10) with |
16723 | | PredictAndBuild_7_superposed_predicted_untrimmed_cycle_2.pdb, chain A (#80), |
16724 | | sequence alignment score = 1461.2 |
16725 | | RMSD between 282 pruned atom pairs is 0.718 angstroms; (across all 283 pairs: |
16726 | | 0.728) |
16727 | | |
16728 | | |
16729 | | > save "/Users/becca/Desktop/Postdoc/COPI notebooks and |
16730 | | > resources/Processing/W20_MM_cyto_COPI/20250706_W20_COPI_cyto_alignments.cxs" |
16731 | | |
16732 | | > show #89 models |
16733 | | |
16734 | | > split #89 chains |
16735 | | |
16736 | | Split fold_20250704_copi_abbprimedgz_trucadg_model_0.cif (#89) into 6 models |
16737 | | Chain information for fold_20250704_copi_abbprimedgz_trucadg_model_0.cif A |
16738 | | #89.1 |
16739 | | --- |
16740 | | Chain | Description |
16741 | | A | No description available |
16742 | | |
16743 | | Chain information for fold_20250704_copi_abbprimedgz_trucadg_model_0.cif B |
16744 | | #89.2 |
16745 | | --- |
16746 | | Chain | Description |
16747 | | B | No description available |
16748 | | |
16749 | | Chain information for fold_20250704_copi_abbprimedgz_trucadg_model_0.cif C |
16750 | | #89.3 |
16751 | | --- |
16752 | | Chain | Description |
16753 | | C | No description available |
16754 | | |
16755 | | Chain information for fold_20250704_copi_abbprimedgz_trucadg_model_0.cif D |
16756 | | #89.4 |
16757 | | --- |
16758 | | Chain | Description |
16759 | | D | No description available |
16760 | | |
16761 | | Chain information for fold_20250704_copi_abbprimedgz_trucadg_model_0.cif E |
16762 | | #89.5 |
16763 | | --- |
16764 | | Chain | Description |
16765 | | E | No description available |
16766 | | |
16767 | | Chain information for fold_20250704_copi_abbprimedgz_trucadg_model_0.cif F |
16768 | | #89.6 |
16769 | | --- |
16770 | | Chain | Description |
16771 | | F | No description available |
16772 | | |
16773 | | |
16774 | | > show #90 models |
16775 | | |
16776 | | > hide #90 models |
16777 | | |
16778 | | > close #90 |
16779 | | |
16780 | | > close #91-93 |
16781 | | |
16782 | | > hide #89.1 models |
16783 | | |
16784 | | > hide #89.2 models |
16785 | | |
16786 | | > show #89.2 models |
16787 | | |
16788 | | > hide #89.3 models |
16789 | | |
16790 | | > hide #89.4 models |
16791 | | |
16792 | | > hide #89.6 models |
16793 | | |
16794 | | > hide #80 models |
16795 | | |
16796 | | > hide #89.2 models |
16797 | | |
16798 | | > hide #!94 models |
16799 | | |
16800 | | > show #!94 models |
16801 | | |
16802 | | > hide #!94 models |
16803 | | |
16804 | | > show #!94 models |
16805 | | |
16806 | | > hide #!94 models |
16807 | | |
16808 | | > show #!94 models |
16809 | | |
16810 | | > hide #!94 models |
16811 | | |
16812 | | > combine #89.5 |
16813 | | |
16814 | | [Repeated 1 time(s)] |
16815 | | |
16816 | | > hide #!89 models |
16817 | | |
16818 | | > hide #!25 models |
16819 | | |
16820 | | > hide #4 models |
16821 | | |
16822 | | Drag select of 2 residues |
16823 | | |
16824 | | > select up |
16825 | | |
16826 | | 41 atoms, 40 bonds, 5 residues, 1 model selected |
16827 | | |
16828 | | > delete sel |
16829 | | |
16830 | | Drag select of 23 residues |
16831 | | |
16832 | | > select up |
16833 | | |
16834 | | 293 atoms, 299 bonds, 37 residues, 1 model selected |
16835 | | |
16836 | | > delete sel |
16837 | | |
16838 | | Drag select of 11 residues, 1 pseudobonds |
16839 | | |
16840 | | > delete sel |
16841 | | |
16842 | | > show #!94 models |
16843 | | |
16844 | | > hide #!94 models |
16845 | | |
16846 | | > show #!94 models |
16847 | | |
16848 | | Drag select of 14 residues |
16849 | | |
16850 | | > select up |
16851 | | |
16852 | | 308 atoms, 312 bonds, 41 residues, 1 model selected |
16853 | | |
16854 | | > delete sel |
16855 | | |
16856 | | > hide #!94 models |
16857 | | |
16858 | | > show #!94 models |
16859 | | |
16860 | | > hide #!94 models |
16861 | | |
16862 | | Drag select of 113 residues |
16863 | | |
16864 | | > select up |
16865 | | |
16866 | | 1028 atoms, 1043 bonds, 131 residues, 1 model selected |
16867 | | |
16868 | | > delete sel |
16869 | | |
16870 | | > fitmap #1 inMap #25 |
16871 | | |
16872 | | Fit molecule copy of fold_20250704_copi_abbprimedgz_trucadg_model_0.cif E (#1) |
16873 | | to map W20_J4027_005_volume_map_gammazeta_local.mrc (#25) using 3497 atoms |
16874 | | average map value = 0.3397, steps = 120 |
16875 | | shifted from previous position = 9.43 |
16876 | | rotated from previous position = 15.4 degrees |
16877 | | atoms outside contour = 1097, contour level = 0.2532 |
16878 | | |
16879 | | Position of copy of fold_20250704_copi_abbprimedgz_trucadg_model_0.cif E (#1) |
16880 | | relative to W20_J4027_005_volume_map_gammazeta_local.mrc (#25) coordinates: |
16881 | | Matrix rotation and translation |
16882 | | 0.28192072 -0.63906244 -0.71562553 141.48853049 |
16883 | | 0.25178912 0.76901734 -0.58754964 138.75926341 |
16884 | | 0.92580935 -0.01454430 0.37771089 139.40543708 |
16885 | | Axis 0.29331865 -0.84024256 0.45602259 |
16886 | | Axis point -12.05331597 0.00000000 214.52876942 |
16887 | | Rotation angle (degrees) 77.62410125 |
16888 | | Shift along axis -11.51818437 |
16889 | | |
16890 | | |
16891 | | > show #!25 models |
16892 | | |
16893 | | > hide #!1 models |
16894 | | |
16895 | | > show #!1 models |
16896 | | |
16897 | | > hide #!1 models |
16898 | | |
16899 | | > show #4 models |
16900 | | |
16901 | | > hide #!25 models |
16902 | | |
16903 | | > hide #4 models |
16904 | | |
16905 | | > show #!1 models |
16906 | | |
16907 | | > show #4 models |
16908 | | |
16909 | | > show #!25 models |
16910 | | |
16911 | | > hide #!1 models |
16912 | | |
16913 | | > hide #!25 models |
16914 | | |
16915 | | > show #!1 models |
16916 | | |
16917 | | > hide #!1 models |
16918 | | |
16919 | | > show #!1 models |
16920 | | |
16921 | | > hide #!1 models |
16922 | | |
16923 | | > hide #4 models |
16924 | | |
16925 | | > show #!1 models |
16926 | | |
16927 | | > hide #!1 models |
16928 | | |
16929 | | > show #4 models |
16930 | | |
16931 | | Drag select of 17 residues |
16932 | | |
16933 | | > select up |
16934 | | |
16935 | | 334 atoms, 340 bonds, 42 residues, 1 model selected |
16936 | | |
16937 | | > delete sel |
16938 | | |
16939 | | Drag select of 9 residues |
16940 | | |
16941 | | > select up |
16942 | | |
16943 | | 183 atoms, 186 bonds, 22 residues, 1 model selected |
16944 | | |
16945 | | > delete sel |
16946 | | |
16947 | | Drag select of 19 residues |
16948 | | |
16949 | | > select up |
16950 | | |
16951 | | 215 atoms, 218 bonds, 30 residues, 1 model selected |
16952 | | |
16953 | | > select clear |
16954 | | |
16955 | | Drag select of 9 residues |
16956 | | |
16957 | | > delete sel |
16958 | | |
16959 | | Drag select of 7 residues |
16960 | | |
16961 | | > select clear |
16962 | | |
16963 | | Drag select of 13 residues |
16964 | | |
16965 | | > select clear |
16966 | | |
16967 | | Drag select of 15 residues |
16968 | | |
16969 | | > delete sel |
16970 | | |
16971 | | > show #!25 models |
16972 | | |
16973 | | > fitmap #4 inMap #25 |
16974 | | |
16975 | | Fit molecule copy of fold_20250704_copi_abbprimedgz_trucadg_model_0.cif E (#4) |
16976 | | to map W20_J4027_005_volume_map_gammazeta_local.mrc (#25) using 4566 atoms |
16977 | | average map value = 0.3172, steps = 112 |
16978 | | shifted from previous position = 8.14 |
16979 | | rotated from previous position = 15.5 degrees |
16980 | | atoms outside contour = 1647, contour level = 0.2532 |
16981 | | |
16982 | | Position of copy of fold_20250704_copi_abbprimedgz_trucadg_model_0.cif E (#4) |
16983 | | relative to W20_J4027_005_volume_map_gammazeta_local.mrc (#25) coordinates: |
16984 | | Matrix rotation and translation |
16985 | | 0.28339787 -0.63805488 -0.71594107 141.54232791 |
16986 | | 0.25944422 0.76971940 -0.58328444 139.21027357 |
16987 | | 0.92324122 -0.02044520 0.38367649 139.06277017 |
16988 | | Axis 0.28838113 -0.83986547 0.45985032 |
16989 | | Axis point -12.84705545 0.00000000 215.45471328 |
16990 | | Rotation angle (degrees) 77.38510832 |
16991 | | Shift along axis -12.15170567 |
16992 | | |
16993 | | |
16994 | | > select #4/E:584 |
16995 | | |
16996 | | 7 atoms, 6 bonds, 1 residue, 1 model selected |
16997 | | |
16998 | | > delete sel |
16999 | | |
17000 | | > select #4/E:583 |
17001 | | |
17002 | | 7 atoms, 6 bonds, 1 residue, 1 model selected |
17003 | | |
17004 | | > delete sel |
17005 | | |
17006 | | > hide #!4 models |
17007 | | |
17008 | | > show #!4 models |
17009 | | |
17010 | | > combine #4 |
17011 | | |
17012 | | > hide #!4 models |
17013 | | |
17014 | | > rename #4 gamma-COPI_v1 |
17015 | | |
17016 | | > combine #64 #66 #67 #70 #71 #4 |
17017 | | |
17018 | | Remapping chain ID 'B' in beta-COPI #66 to 'D' |
17019 | | Remapping chain ID 'C' in beta-COPI #66 to 'E' |
17020 | | Remapping chain ID 'A' in betaprime-COPI #67 to 'F' |
17021 | | Remapping chain ID 'B' in betaprime-COPI #67 to 'G' |
17022 | | Remapping chain ID 'C' in betaprime-COPI #67 to 'H' |
17023 | | Remapping chain ID 'D' in betaprime-COPI #67 to 'I' |
17024 | | Remapping chain ID 'A' in delta-COPI #70 to 'J' |
17025 | | Remapping chain ID 'B' in delta-COPI #70 to 'K' |
17026 | | Remapping chain ID 'A' in zeta-COPI #71 to 'L' |
17027 | | Remapping chain ID 'E' in gamma-COPI_v1 #4 to 'M' |
17028 | | |
17029 | | > hide #!6 models |
17030 | | |
17031 | | > fitmap #4 inMap #25 |
17032 | | |
17033 | | Fit molecule gamma-COPI_v1 (#4) to map |
17034 | | W20_J4027_005_volume_map_gammazeta_local.mrc (#25) using 4552 atoms |
17035 | | average map value = 0.3176, steps = 40 |
17036 | | shifted from previous position = 0.0254 |
17037 | | rotated from previous position = 0.0198 degrees |
17038 | | atoms outside contour = 1637, contour level = 0.2532 |
17039 | | |
17040 | | Position of gamma-COPI_v1 (#4) relative to |
17041 | | W20_J4027_005_volume_map_gammazeta_local.mrc (#25) coordinates: |
17042 | | Matrix rotation and translation |
17043 | | 0.28348444 -0.63781373 -0.71612165 141.56919510 |
17044 | | 0.25922020 0.76992233 -0.58311620 139.19563791 |
17045 | | 0.92327756 -0.02032883 0.38359520 139.07316286 |
17046 | | Axis 0.28836144 -0.83999668 0.45962296 |
17047 | | Axis point -12.85164212 0.00000000 215.45181715 |
17048 | | Rotation angle (degrees) 77.37899637 |
17049 | | Shift along axis -12.17955787 |
17050 | | |
17051 | | |
17052 | | > fitmap #4 inMap #25 |
17053 | | |
17054 | | Fit molecule gamma-COPI_v1 (#4) to map |
17055 | | W20_J4027_005_volume_map_gammazeta_local.mrc (#25) using 4552 atoms |
17056 | | average map value = 0.3176, steps = 40 |
17057 | | shifted from previous position = 0.0216 |
17058 | | rotated from previous position = 0.0256 degrees |
17059 | | atoms outside contour = 1634, contour level = 0.2532 |
17060 | | |
17061 | | Position of gamma-COPI_v1 (#4) relative to |
17062 | | W20_J4027_005_volume_map_gammazeta_local.mrc (#25) coordinates: |
17063 | | Matrix rotation and translation |
17064 | | 0.28351557 -0.63806429 -0.71588608 141.55271505 |
17065 | | 0.25957296 0.76971101 -0.58323823 139.22244867 |
17066 | | 0.92316889 -0.02046756 0.38384929 139.05660502 |
17067 | | Axis 0.28835533 -0.83982741 0.45993599 |
17068 | | Axis point -12.85662194 0.00000000 215.49138830 |
17069 | | Rotation angle (degrees) 77.37682658 |
17070 | | Shift along axis -12.14821127 |
17071 | | |
17072 | | |
17073 | | > fitmap #64 inMap #25 |
17074 | | |
17075 | | Fit molecule alpha-COPI (#64) to map |
17076 | | W20_J4027_005_volume_map_gammazeta_local.mrc (#25) using 6628 atoms |
17077 | | average map value = 0.3537, steps = 72 |
17078 | | shifted from previous position = 0.413 |
17079 | | rotated from previous position = 1.38 degrees |
17080 | | atoms outside contour = 1857, contour level = 0.2532 |
17081 | | |
17082 | | Position of alpha-COPI (#64) relative to |
17083 | | W20_J4027_005_volume_map_gammazeta_local.mrc (#25) coordinates: |
17084 | | Matrix rotation and translation |
17085 | | -0.36586447 -0.91732918 -0.15700440 275.35878138 |
17086 | | -0.79670780 0.22151549 0.56230559 163.87536925 |
17087 | | -0.48104042 0.33081427 -0.81188796 242.97769193 |
17088 | | Axis -0.55633921 0.77875030 0.28988732 |
17089 | | Axis point 211.74531088 0.00000000 70.70797620 |
17090 | | Rotation angle (degrees) 167.99197292 |
17091 | | Shift along axis 44.86125869 |
17092 | | |
17093 | | |
17094 | | > fitmap #66 inMap #25 |
17095 | | |
17096 | | Fit molecule beta-COPI (#66) to map |
17097 | | W20_J4027_005_volume_map_gammazeta_local.mrc (#25) using 6621 atoms |
17098 | | average map value = 0.2922, steps = 64 |
17099 | | shifted from previous position = 0.113 |
17100 | | rotated from previous position = 1.01 degrees |
17101 | | atoms outside contour = 2335, contour level = 0.2532 |
17102 | | |
17103 | | Position of beta-COPI (#66) relative to |
17104 | | W20_J4027_005_volume_map_gammazeta_local.mrc (#25) coordinates: |
17105 | | Matrix rotation and translation |
17106 | | -0.33217082 -0.91999498 -0.20801872 277.50726921 |
17107 | | -0.77605969 0.14122581 0.61464349 158.87975673 |
17108 | | -0.53609132 0.36560157 -0.76088211 242.10904879 |
17109 | | Axis -0.57078454 0.75191666 0.32988838 |
17110 | | Axis point 215.84062714 0.00000000 64.11235965 |
17111 | | Rotation angle (degrees) 167.39915972 |
17112 | | Shift along axis 40.93643893 |
17113 | | |
17114 | | |
17115 | | > fitmap #70 inMap #25 |
17116 | | |
17117 | | Fit molecule delta-COPI (#70) to map |
17118 | | W20_J4027_005_volume_map_gammazeta_local.mrc (#25) using 1431 atoms |
17119 | | average map value = 0.2517, steps = 140 |
17120 | | shifted from previous position = 0.914 |
17121 | | rotated from previous position = 5.42 degrees |
17122 | | atoms outside contour = 770, contour level = 0.2532 |
17123 | | |
17124 | | Position of delta-COPI (#70) relative to |
17125 | | W20_J4027_005_volume_map_gammazeta_local.mrc (#25) coordinates: |
17126 | | Matrix rotation and translation |
17127 | | -0.34798043 -0.93749415 -0.00378617 175.64116001 |
17128 | | 0.05522224 -0.01646557 -0.99833831 178.10409974 |
17129 | | 0.93587399 -0.34761128 0.05750022 154.89993905 |
17130 | | Axis 0.42983493 -0.62068846 0.65573452 |
17131 | | Axis point -39.53177108 213.69242364 0.00000000 |
17132 | | Rotation angle (degrees) 130.80395777 |
17133 | | Shift along axis 66.52278419 |
17134 | | |
17135 | | |
17136 | | > fitmap #71 inMap #25 |
17137 | | |
17138 | | Fit molecule zeta-COPI (#71) to map |
17139 | | W20_J4027_005_volume_map_gammazeta_local.mrc (#25) using 1420 atoms |
17140 | | average map value = 0.2522, steps = 180 |
17141 | | shifted from previous position = 10.5 |
17142 | | rotated from previous position = 19.5 degrees |
17143 | | atoms outside contour = 661, contour level = 0.2532 |
17144 | | |
17145 | | Position of zeta-COPI (#71) relative to |
17146 | | W20_J4027_005_volume_map_gammazeta_local.mrc (#25) coordinates: |
17147 | | Matrix rotation and translation |
17148 | | -0.32469740 0.40326773 0.85553887 117.00818860 |
17149 | | 0.20978479 -0.85131177 0.48089357 145.18762928 |
17150 | | 0.92225916 0.33562393 0.19181929 99.65953140 |
17151 | | Axis -0.57881168 -0.26584003 -0.77091253 |
17152 | | Axis point 25.03383553 54.96126454 0.00000000 |
17153 | | Rotation angle (degrees) 172.79097233 |
17154 | | Shift along axis -183.15117020 |
17155 | | |
17156 | | |
17157 | | > show #71 models |
17158 | | |
17159 | | > hide #71 models |
17160 | | |
17161 | | > show #71 models |
17162 | | |
17163 | | > hide #71 models |
17164 | | |
17165 | | > close #8 |
17166 | | |
17167 | | > combine #64 #66 #67 #70 #71 #4 |
17168 | | |
17169 | | Remapping chain ID 'B' in beta-COPI #66 to 'D' |
17170 | | Remapping chain ID 'C' in beta-COPI #66 to 'E' |
17171 | | Remapping chain ID 'A' in betaprime-COPI #67 to 'F' |
17172 | | Remapping chain ID 'B' in betaprime-COPI #67 to 'G' |
17173 | | Remapping chain ID 'C' in betaprime-COPI #67 to 'H' |
17174 | | Remapping chain ID 'D' in betaprime-COPI #67 to 'I' |
17175 | | Remapping chain ID 'A' in delta-COPI #70 to 'J' |
17176 | | Remapping chain ID 'B' in delta-COPI #70 to 'K' |
17177 | | Remapping chain ID 'A' in zeta-COPI #71 to 'L' |
17178 | | Remapping chain ID 'E' in gamma-COPI_v1 #4 to 'M' |
17179 | | |
17180 | | > rename #8 COPI_cyto_for_J4027_v1 |
17181 | | |
17182 | | > cd "/Users/becca/Desktop/Postdoc/COPI notebooks and |
17183 | | > resources/Processing/W20_MM_cyto_COPI" |
17184 | | |
17185 | | Current working directory is: /Users/becca/Desktop/Postdoc/COPI notebooks and |
17186 | | resources/Processing/W20_MM_cyto_COPI |
17187 | | |
17188 | | > save COPI_cyto_for_J4027_v1.pdb #8 |
17189 | | |
17190 | | > open |
17191 | | > /Users/becca/Desktop/Postdoc/Phenix/W20_MM_cyto_COPI/CombineFocusedMaps_8/cc_map.ccp4 |
17192 | | |
17193 | | Opened cc_map.ccp4 as #10, grid size 240,240,240, pixel 1.19, shown at level |
17194 | | 0.697, step 1, values float32 |
17195 | | |
17196 | | > open |
17197 | | > /Users/becca/Desktop/Postdoc/Phenix/W20_MM_cyto_COPI/CombineFocusedMaps_8/composite_map.ccp4 |
17198 | | |
17199 | | Opened composite_map.ccp4 as #12, grid size 240,240,240, pixel 1.19, shown at |
17200 | | level 5.35, step 1, values float32 |
17201 | | |
17202 | | > open |
17203 | | > /Users/becca/Desktop/Postdoc/Phenix/W20_MM_cyto_COPI/CombineFocusedMaps_8/composite_map.pdb |
17204 | | |
17205 | | Summary of feedback from opening |
17206 | | /Users/becca/Desktop/Postdoc/Phenix/W20_MM_cyto_COPI/CombineFocusedMaps_8/composite_map.pdb |
17207 | | --- |
17208 | | warning | Ignored bad PDB record found on line 27366 |
17209 | | |
17210 | | Chain information for composite_map.pdb #14 |
17211 | | --- |
17212 | | Chain | Description |
17213 | | A | No description available |
17214 | | B | No description available |
17215 | | C | No description available |
17216 | | D | No description available |
17217 | | E | No description available |
17218 | | F | No description available |
17219 | | G | No description available |
17220 | | H | No description available |
17221 | | I | No description available |
17222 | | J | No description available |
17223 | | K | No description available |
17224 | | L | No description available |
17225 | | M | No description available |
17226 | | |
17227 | | |
17228 | | > open |
17229 | | > /Users/becca/Desktop/Postdoc/Phenix/W20_MM_cyto_COPI/CombineFocusedMaps_8/contribution_map_1.ccp4 |
17230 | | |
17231 | | Opened contribution_map_1.ccp4 as #16, grid size 240,240,240, pixel 1.19, |
17232 | | shown at level 1, step 1, values float32 |
17233 | | |
17234 | | > open |
17235 | | > /Users/becca/Desktop/Postdoc/Phenix/W20_MM_cyto_COPI/CombineFocusedMaps_8/contribution_map_2.ccp4 |
17236 | | |
17237 | | Opened contribution_map_2.ccp4 as #18, grid size 240,240,240, pixel 1.19, |
17238 | | shown at level 0.47, step 1, values float32 |
17239 | | |
17240 | | > hide #!8 models |
17241 | | |
17242 | | > hide #!10 models |
17243 | | |
17244 | | > hide #!12 models |
17245 | | |
17246 | | > hide #!14 models |
17247 | | |
17248 | | > hide #!16 models |
17249 | | |
17250 | | > hide #!18 models |
17251 | | |
17252 | | > hide #!25 models |
17253 | | |
17254 | | > show #!10 models |
17255 | | |
17256 | | > show #!12 models |
17257 | | |
17258 | | > show #!14 models |
17259 | | |
17260 | | > show #!16 models |
17261 | | |
17262 | | > show #!18 models |
17263 | | |
17264 | | > close #18 |
17265 | | |
17266 | | > hide #!14 models |
17267 | | |
17268 | | > close #10 |
17269 | | |
17270 | | > close #12 |
17271 | | |
17272 | | > show #!14 models |
17273 | | |
17274 | | > hide #!14 models |
17275 | | |
17276 | | > hide #!16 models |
17277 | | |
17278 | | > show #!16 models |
17279 | | |
17280 | | > close #16 |
17281 | | |
17282 | | > close #14 |
17283 | | |
17284 | | > show #!4 models |
17285 | | |
17286 | | > show #!25 models |
17287 | | |
17288 | | > hide #!25 models |
17289 | | |
17290 | | > show #!23 models |
17291 | | |
17292 | | > show #!27 models |
17293 | | |
17294 | | > show #!25 models |
17295 | | |
17296 | | > hide #!27 models |
17297 | | |
17298 | | > show #!32 models |
17299 | | |
17300 | | > hide #!32 models |
17301 | | |
17302 | | > show #!33 models |
17303 | | |
17304 | | > hide #!25 models |
17305 | | |
17306 | | > hide #!23 models |
17307 | | |
17308 | | > hide #!33 models |
17309 | | |
17310 | | > show #!2 models |
17311 | | |
17312 | | > hide #!2 models |
17313 | | |
17314 | | > show #!3 models |
17315 | | |
17316 | | > close #3 |
17317 | | |
17318 | | > show #!5 models |
17319 | | |
17320 | | > close #5 |
17321 | | |
17322 | | > show #!7 models |
17323 | | |
17324 | | > hide #!7 models |
17325 | | |
17326 | | > show #!9 models |
17327 | | |
17328 | | > show #!23 models |
17329 | | |
17330 | | > show #!25 models |
17331 | | |
17332 | | > show #!27 models |
17333 | | |
17334 | | > hide #!9 models |
17335 | | |
17336 | | > show #!9 models |
17337 | | |
17338 | | > hide #!9 models |
17339 | | |
17340 | | > hide #!23 models |
17341 | | |
17342 | | > hide #!27 models |
17343 | | |
17344 | | > hide #!25 models |
17345 | | |
17346 | | > show #!7 models |
17347 | | |
17348 | | > hide #!7 models |
17349 | | |
17350 | | > show #!9 models |
17351 | | |
17352 | | > hide #!9 models |
17353 | | |
17354 | | > show #!11 models |
17355 | | |
17356 | | > close #11 |
17357 | | |
17358 | | > show #!13 models |
17359 | | |
17360 | | > show #!15 models |
17361 | | |
17362 | | > hide #!15 models |
17363 | | |
17364 | | > show #!17 models |
17365 | | |
17366 | | > hide #!17 models |
17367 | | |
17368 | | > show #!19 models |
17369 | | |
17370 | | > show #!17 models |
17371 | | |
17372 | | > show #!15 models |
17373 | | |
17374 | | > show #!21 models |
17375 | | |
17376 | | > hide #!21 models |
17377 | | |
17378 | | > hide #!19 models |
17379 | | |
17380 | | > hide #!17 models |
17381 | | |
17382 | | > hide #!15 models |
17383 | | |
17384 | | > hide #!13 models |
17385 | | |
17386 | | > show #!29 models |
17387 | | |
17388 | | > hide #!29 models |
17389 | | |
17390 | | > show #!30 models |
17391 | | |
17392 | | > show #!29 models |
17393 | | |
17394 | | > show #!31 models |
17395 | | |
17396 | | > hide #!31 models |
17397 | | |
17398 | | > hide #!30 models |
17399 | | |
17400 | | > hide #!29 models |
17401 | | |
17402 | | > show #!32 models |
17403 | | |
17404 | | > hide #!4 models |
17405 | | |
17406 | | > hide #!32 models |
17407 | | |
17408 | | > show #!13 models |
17409 | | |
17410 | | > show #!15 models |
17411 | | |
17412 | | > hide #!15 models |
17413 | | |
17414 | | > show #!17 models |
17415 | | |
17416 | | > hide #!17 models |
17417 | | |
17418 | | > show #!19 models |
17419 | | |
17420 | | > show #!21 models |
17421 | | |
17422 | | > hide #!21 models |
17423 | | |
17424 | | > hide #!19 models |
17425 | | |
17426 | | > hide #!13 models |
17427 | | |
17428 | | > hide #!2 models |
17429 | | |
17430 | | > show #!2 models |
17431 | | |
17432 | | > hide #!2 models |
17433 | | |
17434 | | > show #!1 models |
17435 | | |
17436 | | > hide #!1 models |
17437 | | |
17438 | | > show #!4 models |
17439 | | |
17440 | | > show #!7 models |
17441 | | |
17442 | | > hide #!7 models |
17443 | | |
17444 | | > close #1 |
17445 | | |
17446 | | > hide #!4 models |
17447 | | |
17448 | | > open "/Users/becca/Desktop/Postdoc/COPI notebooks and |
17449 | | > resources/Processing/W20_MM_cyto_COPI/cryosparc_P126_J3623_008_volume_map_half_A.mrc" |
17450 | | > "/Users/becca/Desktop/Postdoc/COPI notebooks and |
17451 | | > resources/Processing/W20_MM_cyto_COPI/cryosparc_P126_J3623_008_volume_map_half_B.mrc" |
17452 | | |
17453 | | Opened cryosparc_P126_J3623_008_volume_map_half_A.mrc as #1.1, grid size |
17454 | | 240,240,240, pixel 1.19, shown at level 0.256, step 1, values float32 |
17455 | | Opened cryosparc_P126_J3623_008_volume_map_half_B.mrc as #1.2, grid size |
17456 | | 240,240,240, pixel 1.19, shown at level 0.257, step 1, values float32 |
17457 | | |
17458 | | > hide #!1.1 models |
17459 | | |
17460 | | > show #!1.1 models |
17461 | | |
17462 | | > hide #!1.2 models |
17463 | | |
17464 | | > show #!1.2 models |
17465 | | |
17466 | | > show #!2 models |
17467 | | |
17468 | | > hide #!2 models |
17469 | | |
17470 | | > show #!2 models |
17471 | | |
17472 | | > volume #1.1 level 0.2608 |
17473 | | |
17474 | | > volume #1.2 level 0.2582 |
17475 | | |
17476 | | > hide #!1 models |
17477 | | |
17478 | | > close #1 |
17479 | | |
17480 | | > open "/Users/becca/Desktop/Postdoc/COPI notebooks and |
17481 | | > resources/Processing/W20_MM_cyto_COPI/J3623_auto_sharpened_map.ccp4" |
17482 | | |
17483 | | Opened J3623_auto_sharpened_map.ccp4 as #1, grid size 240,240,240, pixel 1.19, |
17484 | | shown at level 3.17, step 1, values float32 |
17485 | | |
17486 | | > hide #!2 models |
17487 | | |
17488 | | > volume #1 level 2.641 |
17489 | | |
17490 | | > hide #!1 models |
17491 | | |
17492 | | > show #!1 models |
17493 | | |
17494 | | > show #!2 models |
17495 | | |
17496 | | > hide #!1 models |
17497 | | |
17498 | | > show #!1 models |
17499 | | |
17500 | | > open |
17501 | | > /Users/becca/Desktop/Postdoc/Phenix/W20_MM_cyto_COPI/ResolveCryoEM_22/denmod_resolution_map.ccp4 |
17502 | | |
17503 | | Opened denmod_resolution_map.ccp4 as #3, grid size 104,126,128, pixel 1.19, |
17504 | | shown at level 11.3, step 1, values float32 |
17505 | | |
17506 | | > hide #!3 models |
17507 | | |
17508 | | > show #!3 models |
17509 | | |
17510 | | > hide #!2 models |
17511 | | |
17512 | | > hide #!1 models |
17513 | | |
17514 | | > hide #!3 models |
17515 | | |
17516 | | > close #3 |
17517 | | |
17518 | | > open |
17519 | | > /Users/becca/Desktop/Postdoc/Phenix/W20_MM_cyto_COPI/ResolveCryoEM_22/J3623_denmod_map.ccp4 |
17520 | | |
17521 | | Opened J3623_denmod_map.ccp4 as #3, grid size 104,126,128, pixel 1.19, shown |
17522 | | at level 0.531, step 1, values float32 |
17523 | | |
17524 | | > volume #3 level 0.2174 |
17525 | | |
17526 | | > show #!66 models |
17527 | | |
17528 | | > show #!64 models |
17529 | | |
17530 | | > hide #!64 models |
17531 | | |
17532 | | > show #!64 models |
17533 | | |
17534 | | > hide #!66 models |
17535 | | |
17536 | | > hide #!64 models |
17537 | | |
17538 | | > show #!70 models |
17539 | | |
17540 | | > volume #3 level 0.1325 |
17541 | | |
17542 | | > volume #3 level 0.25 |
17543 | | |
17544 | | > show #!66 models |
17545 | | |
17546 | | > color #5 gammav3 |
17547 | | |
17548 | | > color #4 gammav3 |
17549 | | |
17550 | | > hide #!3 models |
17551 | | |
17552 | | > close #3 |
17553 | | |
17554 | | > close #2 |
17555 | | |
17556 | | > close #1 |
17557 | | |
17558 | | > open "/Users/becca/Desktop/Postdoc/COPI notebooks and |
17559 | | > resources/Processing/W20_MM_cyto_COPI/W20_J3623_consensus_structure.mrc" |
17560 | | |
17561 | | Opened W20_J3623_consensus_structure.mrc as #1, grid size 240,240,240, pixel |
17562 | | 1.19, shown at level 0.257, step 1, values float32 |
17563 | | |
17564 | | > open "/Users/becca/Desktop/Postdoc/COPI notebooks and |
17565 | | > resources/Processing/W20_MM_cyto_COPI/J3623_auto_sharpened_map.ccp4" |
17566 | | |
17567 | | Opened J3623_auto_sharpened_map.ccp4 as #2, grid size 240,240,240, pixel 1.19, |
17568 | | shown at level 3.17, step 1, values float32 |
17569 | | |
17570 | | > hide #!70 models |
17571 | | |
17572 | | > hide #!66 models |
17573 | | |
17574 | | > open "/Users/becca/Desktop/Postdoc/COPI notebooks and |
17575 | | > resources/Processing/W20_MM_cyto_COPI/cryosparc_P126_J3623_008_volume_map_half_A.mrc" |
17576 | | > "/Users/becca/Desktop/Postdoc/COPI notebooks and |
17577 | | > resources/Processing/W20_MM_cyto_COPI/cryosparc_P126_J3623_008_volume_map_half_B.mrc" |
17578 | | |
17579 | | Opened cryosparc_P126_J3623_008_volume_map_half_A.mrc as #3.1, grid size |
17580 | | 240,240,240, pixel 1.19, shown at level 0.256, step 1, values float32 |
17581 | | Opened cryosparc_P126_J3623_008_volume_map_half_B.mrc as #3.2, grid size |
17582 | | 240,240,240, pixel 1.19, shown at level 0.257, step 1, values float32 |
17583 | | |
17584 | | > rename #3 J3623_half_maps |
17585 | | |
17586 | | > combine #4 |
17587 | | |
17588 | | > close #4 |
17589 | | |
17590 | | > combine #5 |
17591 | | |
17592 | | [Repeated 1 time(s)] |
17593 | | |
17594 | | > close #4 |
17595 | | |
17596 | | > close #5 |
17597 | | |
17598 | | > show #!6 models |
17599 | | |
17600 | | > hide #!6 models |
17601 | | |
17602 | | > close #6 |
17603 | | |
17604 | | > show #!7 models |
17605 | | |
17606 | | > hide #!7 models |
17607 | | |
17608 | | > close #7 |
17609 | | |
17610 | | > open "/Users/becca/Desktop/Postdoc/COPI notebooks and |
17611 | | > resources/Processing/W20_MM_cyto_COPI/W20_J3987_004_volume_map_csparc_refine_of_relion_job125_bprime_mask.mrc" |
17612 | | |
17613 | | Opened W20_J3987_004_volume_map_csparc_refine_of_relion_job125_bprime_mask.mrc |
17614 | | as #4, grid size 240,240,240, pixel 1.2, shown at level 0.273, step 1, values |
17615 | | float32 |
17616 | | |
17617 | | > select add #4 |
17618 | | |
17619 | | 2 models selected |
17620 | | |
17621 | | > ui mousemode right "rotate selected models" |
17622 | | |
17623 | | > view matrix models |
17624 | | > #4,-0.25307,-0.96623,-0.048581,315.98,-0.86086,0.202,0.46702,167.97,-0.44144,0.16001,-0.88291,294.43 |
17625 | | |
17626 | | > view matrix models |
17627 | | > #4,-0.14459,-0.98817,-0.051169,305.09,-0.67606,0.060895,0.73433,127.29,-0.72252,0.14077,-0.67686,306.41 |
17628 | | |
17629 | | > ui mousemode right "translate selected models" |
17630 | | |
17631 | | > view matrix models |
17632 | | > #4,-0.14459,-0.98817,-0.051169,320.79,-0.67606,0.060895,0.73433,129.24,-0.72252,0.14077,-0.67686,326.78 |
17633 | | |
17634 | | > fitmap #4 inMap #1 |
17635 | | |
17636 | | Fit map |
17637 | | W20_J3987_004_volume_map_csparc_refine_of_relion_job125_bprime_mask.mrc in map |
17638 | | W20_J3623_consensus_structure.mrc using 138206 points |
17639 | | correlation = 0.982, correlation about mean = 0.7994, overlap = 2.043e+04 |
17640 | | steps = 156, shift = 6.09, angle = 17.9 degrees |
17641 | | |
17642 | | Position of |
17643 | | W20_J3987_004_volume_map_csparc_refine_of_relion_job125_bprime_mask.mrc (#4) |
17644 | | relative to W20_J3623_consensus_structure.mrc (#1) coordinates: |
17645 | | Matrix rotation and translation |
17646 | | -0.33998516 -0.91904572 -0.19941175 353.84995809 |
17647 | | -0.77678119 0.15490951 0.61042118 146.12290386 |
17648 | | -0.53011420 0.36243344 -0.76656437 277.61520820 |
17649 | | Axis -0.56728131 0.75649433 0.32543548 |
17650 | | Axis point 252.00716027 0.00000000 81.49850043 |
17651 | | Rotation angle (degrees) 167.37461303 |
17652 | | Shift along axis 0.15451903 |
17653 | | |
17654 | | |
17655 | | > volume resample #4 onGrid #1 |
17656 | | |
17657 | | Opened W20_J3987_004_volume_map_csparc_refine_of_relion_job125_bprime_mask.mrc |
17658 | | resampled as #5, grid size 240,240,240, pixel 1.19, shown at step 1, values |
17659 | | float32 |
17660 | | |
17661 | | > select subtract #4 |
17662 | | |
17663 | | Nothing selected |
17664 | | |
17665 | | > close #4 |
17666 | | |
17667 | | > cd "/Users/becca/Desktop/Postdoc/COPI notebooks and |
17668 | | > resources/Processing/W20_MM_cyto_COPI" |
17669 | | |
17670 | | Current working directory is: /Users/becca/Desktop/Postdoc/COPI notebooks and |
17671 | | resources/Processing/W20_MM_cyto_COPI |
17672 | | |
17673 | | > save W20_J3987_consensus_resampled.mrc #5 |
17674 | | |
17675 | | > open "/Users/becca/Desktop/Postdoc/COPI notebooks and |
17676 | | > resources/Processing/W20_MM_cyto_COPI/W20_J3987_consensus_resampled.mrc" |
17677 | | |
17678 | | Opened W20_J3987_consensus_resampled.mrc as #4, grid size 240,240,240, pixel |
17679 | | 1.19, shown at level 0.277, step 1, values float32 |
17680 | | |
17681 | | > hide #!5 models |
17682 | | |
17683 | | > fitmap #4 inMap #1 |
17684 | | |
17685 | | Fit map W20_J3987_consensus_resampled.mrc in map |
17686 | | W20_J3623_consensus_structure.mrc using 138117 points |
17687 | | correlation = 0.9803, correlation about mean = 0.7915, overlap = 2.075e+04 |
17688 | | steps = 28, shift = 0.021, angle = 0.00696 degrees |
17689 | | |
17690 | | Position of W20_J3987_consensus_resampled.mrc (#4) relative to |
17691 | | W20_J3623_consensus_structure.mrc (#1) coordinates: |
17692 | | Matrix rotation and translation |
17693 | | 1.00000000 0.00006999 -0.00003626 -0.00018519 |
17694 | | -0.00006999 0.99999999 -0.00009239 0.04429622 |
17695 | | 0.00003626 0.00009240 1.00000000 -0.01684920 |
17696 | | Axis 0.76076686 -0.29856000 -0.57627746 |
17697 | | Axis point 0.00000000 205.18615312 467.59873778 |
17698 | | Rotation angle (degrees) 0.00695857 |
17699 | | Shift along axis -0.00365615 |
17700 | | |
17701 | | |
17702 | | > close #5 |
17703 | | |
17704 | | > open "/Users/becca/Desktop/Postdoc/COPI notebooks and |
17705 | | > resources/Processing/W20_MM_cyto_COPI/cryosparc_P126_J3987_004_volume_map_half_B.mrc" |
17706 | | > "/Users/becca/Desktop/Postdoc/COPI notebooks and |
17707 | | > resources/Processing/W20_MM_cyto_COPI/cryosparc_P126_J3987_004_volume_map_half_A.mrc" |
17708 | | |
17709 | | Opened cryosparc_P126_J3987_004_volume_map_half_B.mrc as #5.1, grid size |
17710 | | 240,240,240, pixel 1.2, shown at level 0.274, step 1, values float32 |
17711 | | Opened cryosparc_P126_J3987_004_volume_map_half_A.mrc as #5.2, grid size |
17712 | | 240,240,240, pixel 1.2, shown at level 0.274, step 1, values float32 |
17713 | | |
17714 | | > select add #5.1 |
17715 | | |
17716 | | 2 models selected |
17717 | | |
17718 | | > select add #5.2 |
17719 | | |
17720 | | 4 models selected |
17721 | | |
17722 | | > ui mousemode right "rotate selected models" |
17723 | | |
17724 | | > view matrix models |
17725 | | > #5.1,-0.38208,-0.71776,-0.5821,371.56,-0.90525,0.41738,0.079546,196.1,0.18586,0.55733,-0.80922,143.66,#5.2,-0.38208,-0.71776,-0.5821,371.56,-0.90525,0.41738,0.079546,196.1,0.18586,0.55733,-0.80922,143.66 |
17726 | | |
17727 | | > view matrix models |
17728 | | > #5.1,-0.42487,-0.88152,-0.20596,349.25,-0.72637,0.19619,0.6587,124.33,-0.54025,0.42946,-0.72366,248.21,#5.2,-0.42487,-0.88152,-0.20596,349.25,-0.72637,0.19619,0.6587,124.33,-0.54025,0.42946,-0.72366,248.21 |
17729 | | |
17730 | | > ui mousemode right "translate selected models" |
17731 | | |
17732 | | > view matrix models |
17733 | | > #5.1,-0.42487,-0.88152,-0.20596,359.35,-0.72637,0.19619,0.6587,126.62,-0.54025,0.42946,-0.72366,262.21,#5.2,-0.42487,-0.88152,-0.20596,359.35,-0.72637,0.19619,0.6587,126.62,-0.54025,0.42946,-0.72366,262.21 |
17734 | | |
17735 | | > fitmap #5.1 inMap #1 |
17736 | | |
17737 | | Fit map cryosparc_P126_J3987_004_volume_map_half_B.mrc in map |
17738 | | W20_J3623_consensus_structure.mrc using 138219 points |
17739 | | correlation = 0.9769, correlation about mean = 0.7541, overlap = 2.056e+04 |
17740 | | steps = 80, shift = 1.82, angle = 5.92 degrees |
17741 | | |
17742 | | Position of cryosparc_P126_J3987_004_volume_map_half_B.mrc (#5.1) relative to |
17743 | | W20_J3623_consensus_structure.mrc (#1) coordinates: |
17744 | | Matrix rotation and translation |
17745 | | -0.34088191 -0.91883476 -0.19885219 353.85897435 |
17746 | | -0.77626012 0.15578649 0.61086069 145.85323816 |
17747 | | -0.53030155 0.36259239 -0.76635958 277.59067344 |
17748 | | Axis -0.56685311 0.75677442 0.32553039 |
17749 | | Axis point 251.87895379 0.00000000 81.50551240 |
17750 | | Rotation angle (degrees) 167.35038692 |
17751 | | Shift along axis 0.15614218 |
17752 | | |
17753 | | |
17754 | | > fitmap #5.2 inMap #1 |
17755 | | |
17756 | | Fit map cryosparc_P126_J3987_004_volume_map_half_A.mrc in map |
17757 | | W20_J3623_consensus_structure.mrc using 138231 points |
17758 | | correlation = 0.9769, correlation about mean = 0.7539, overlap = 2.061e+04 |
17759 | | steps = 80, shift = 1.81, angle = 6.04 degrees |
17760 | | |
17761 | | Position of cryosparc_P126_J3987_004_volume_map_half_A.mrc (#5.2) relative to |
17762 | | W20_J3623_consensus_structure.mrc (#1) coordinates: |
17763 | | Matrix rotation and translation |
17764 | | -0.33893365 -0.91937774 -0.19967107 353.79911788 |
17765 | | -0.77742964 0.15417077 0.60978235 146.41544867 |
17766 | | -0.52983688 0.36190597 -0.76700517 277.70182392 |
17767 | | Axis -0.56776952 0.75625634 0.32513709 |
17768 | | Axis point 252.14174450 0.00000000 81.52863602 |
17769 | | Rotation angle (degrees) 167.39140634 |
17770 | | Shift along axis 0.14242009 |
17771 | | |
17772 | | |
17773 | | > select subtract #5.2 |
17774 | | |
17775 | | 2 models selected |
17776 | | |
17777 | | > select subtract #5.1 |
17778 | | |
17779 | | Nothing selected |
17780 | | |
17781 | | > volume resample #5.1 #5.2 onGrid #1 |
17782 | | |
17783 | | Opened cryosparc_P126_J3987_004_volume_map_half_B.mrc resampled as #6, grid |
17784 | | size 240,240,240, pixel 1.19, shown at step 1, values float32 |
17785 | | Opened cryosparc_P126_J3987_004_volume_map_half_A.mrc resampled as #7, grid |
17786 | | size 240,240,240, pixel 1.19, shown at step 1, values float32 |
17787 | | |
17788 | | > save W20_J3987_halfA_consensus_resampled.mrc #7 |
17789 | | |
17790 | | > save W20_J3987_halfB_consensus_resampled.mrc #6 |
17791 | | |
17792 | | > close #5-7#5.1-2 |
17793 | | |
17794 | | > open "/Users/becca/Desktop/Postdoc/COPI notebooks and |
17795 | | > resources/Processing/W20_MM_cyto_COPI/W20_J3987_halfA_consensus_resampled.mrc" |
17796 | | > "/Users/becca/Desktop/Postdoc/COPI notebooks and |
17797 | | > resources/Processing/W20_MM_cyto_COPI/W20_J3987_halfB_consensus_resampled.mrc" |
17798 | | |
17799 | | Opened W20_J3987_halfA_consensus_resampled.mrc as #5.1, grid size 240,240,240, |
17800 | | pixel 1.19, shown at level 0.276, step 1, values float32 |
17801 | | Opened W20_J3987_halfB_consensus_resampled.mrc as #5.2, grid size 240,240,240, |
17802 | | pixel 1.19, shown at level 0.276, step 1, values float32 |
17803 | | |
17804 | | > rename #5 J3987_half_maps |
17805 | | |
17806 | | > show #!8 models |
17807 | | |
17808 | | > hide #!8 models |
17809 | | |
17810 | | > combine #8 |
17811 | | |
17812 | | [Repeated 2 time(s)] |
17813 | | |
17814 | | > close #6-8 |
17815 | | |
17816 | | > open "/Users/becca/Desktop/Postdoc/COPI notebooks and |
17817 | | > resources/Processing/W20_MM_cyto_COPI/W20_J4021_005_volume_map_bprime_local.mrc" |
17818 | | |
17819 | | Opened W20_J4021_005_volume_map_bprime_local.mrc as #6, grid size 240,240,240, |
17820 | | pixel 1.19, shown at level 0.26, step 1, values float32 |
17821 | | |
17822 | | > open "/Users/becca/Desktop/Postdoc/COPI notebooks and |
17823 | | > resources/Processing/W20_MM_cyto_COPI/cryosparc_P126_J4021_005_volume_map_half_B.mrc" |
17824 | | |
17825 | | Opened cryosparc_P126_J4021_005_volume_map_half_B.mrc as #7, grid size |
17826 | | 240,240,240, pixel 1.19, shown at level 0.263, step 1, values float32 |
17827 | | |
17828 | | > open "/Users/becca/Desktop/Postdoc/COPI notebooks and |
17829 | | > resources/Processing/W20_MM_cyto_COPI/cryosparc_P126_J4021_005_volume_map_half_A.mrc" |
17830 | | |
17831 | | Opened cryosparc_P126_J4021_005_volume_map_half_A.mrc as #8, grid size |
17832 | | 240,240,240, pixel 1.19, shown at level 0.262, step 1, values float32 |
17833 | | |
17834 | | > volume resample #6 #7 #8 onGrid #1 |
17835 | | |
17836 | | Opened W20_J4021_005_volume_map_bprime_local.mrc resampled as #12, grid size |
17837 | | 240,240,240, pixel 1.19, shown at step 1, values float32 |
17838 | | Opened cryosparc_P126_J4021_005_volume_map_half_B.mrc resampled as #14, grid |
17839 | | size 240,240,240, pixel 1.19, shown at step 1, values float32 |
17840 | | Opened cryosparc_P126_J4021_005_volume_map_half_A.mrc resampled as #16, grid |
17841 | | size 240,240,240, pixel 1.19, shown at step 1, values float32 |
17842 | | |
17843 | | > hide #!16 models |
17844 | | |
17845 | | > show #!16 models |
17846 | | |
17847 | | > hide #!16 models |
17848 | | |
17849 | | > show #!16 models |
17850 | | |
17851 | | > hide #!16 models |
17852 | | |
17853 | | > show #!16 models |
17854 | | |
17855 | | > close #16 |
17856 | | |
17857 | | > close #14 |
17858 | | |
17859 | | > close #12 |
17860 | | |
17861 | | > hide #!5 models |
17862 | | |
17863 | | > hide #!4 models |
17864 | | |
17865 | | > hide #!3 models |
17866 | | |
17867 | | > hide #!2 models |
17868 | | |
17869 | | > fitmap #6 #7 #8 inMap #1 |
17870 | | |
17871 | | Multiple maps for #6#7#8 |
17872 | | |
17873 | | > show #!6 models |
17874 | | |
17875 | | > show #!7 models |
17876 | | |
17877 | | > show #!8 models |
17878 | | |
17879 | | > fitmap #6 inMap #1 |
17880 | | |
17881 | | Fit map W20_J4021_005_volume_map_bprime_local.mrc in map |
17882 | | W20_J3623_consensus_structure.mrc using 138166 points |
17883 | | correlation = 0.9132, correlation about mean = 0.2137, overlap = 1.684e+04 |
17884 | | steps = 52, shift = 0.553, angle = 0.278 degrees |
17885 | | |
17886 | | Position of W20_J4021_005_volume_map_bprime_local.mrc (#6) relative to |
17887 | | W20_J3623_consensus_structure.mrc (#1) coordinates: |
17888 | | Matrix rotation and translation |
17889 | | 0.99999230 0.00368913 -0.00133441 -0.32621193 |
17890 | | -0.00369294 0.99998910 -0.00285852 0.49731748 |
17891 | | 0.00132385 0.00286342 0.99999502 -0.37762592 |
17892 | | Axis 0.58922795 -0.27373864 -0.76018260 |
17893 | | Axis point 131.36464990 82.10529237 0.00000000 |
17894 | | Rotation angle (degrees) 0.27819836 |
17895 | | Shift along axis -0.04128355 |
17896 | | |
17897 | | |
17898 | | > fitmap #7 inMap #1 |
17899 | | |
17900 | | Fit map cryosparc_P126_J4021_005_volume_map_half_B.mrc in map |
17901 | | W20_J3623_consensus_structure.mrc using 138066 points |
17902 | | correlation = 0.907, correlation about mean = 0.2359, overlap = 1.707e+04 |
17903 | | steps = 68, shift = 0.546, angle = 0.314 degrees |
17904 | | |
17905 | | Position of cryosparc_P126_J4021_005_volume_map_half_B.mrc (#7) relative to |
17906 | | W20_J3623_consensus_structure.mrc (#1) coordinates: |
17907 | | Matrix rotation and translation |
17908 | | 0.99999065 0.00386110 -0.00194507 -0.25775142 |
17909 | | -0.00386765 0.99998683 -0.00337455 0.61622992 |
17910 | | 0.00193201 0.00338205 0.99999241 -0.53165616 |
17911 | | Axis 0.61572178 -0.35331456 -0.70431208 |
17912 | | Axis point 158.92151900 66.82584882 0.00000000 |
17913 | | Rotation angle (degrees) 0.31436809 |
17914 | | Shift along axis -0.00197431 |
17915 | | |
17916 | | |
17917 | | > fitmap #8 inMap #1 |
17918 | | |
17919 | | Fit map cryosparc_P126_J4021_005_volume_map_half_A.mrc in map |
17920 | | W20_J3623_consensus_structure.mrc using 138185 points |
17921 | | correlation = 0.9063, correlation about mean = 0.2362, overlap = 1.703e+04 |
17922 | | steps = 44, shift = 0.534, angle = 0.288 degrees |
17923 | | |
17924 | | Position of cryosparc_P126_J4021_005_volume_map_half_A.mrc (#8) relative to |
17925 | | W20_J3623_consensus_structure.mrc (#1) coordinates: |
17926 | | Matrix rotation and translation |
17927 | | 0.99999293 0.00344362 -0.00151137 -0.26561114 |
17928 | | -0.00344864 0.99998850 -0.00333361 0.55833592 |
17929 | | 0.00149988 0.00333880 0.99999330 -0.46926499 |
17930 | | Axis 0.66362765 -0.29949438 -0.68549359 |
17931 | | Axis point 159.76195443 73.25690596 0.00000000 |
17932 | | Rotation angle (degrees) 0.28804014 |
17933 | | Shift along axis -0.02180722 |
17934 | | |
17935 | | |
17936 | | > volume resample #6 #7 #8 onGrid #1 |
17937 | | |
17938 | | Opened W20_J4021_005_volume_map_bprime_local.mrc resampled as #12, grid size |
17939 | | 240,240,240, pixel 1.19, shown at step 1, values float32 |
17940 | | Opened cryosparc_P126_J4021_005_volume_map_half_B.mrc resampled as #14, grid |
17941 | | size 240,240,240, pixel 1.19, shown at step 1, values float32 |
17942 | | Opened cryosparc_P126_J4021_005_volume_map_half_A.mrc resampled as #16, grid |
17943 | | size 240,240,240, pixel 1.19, shown at step 1, values float32 |
17944 | | |
17945 | | > hide #!10 models |
17946 | | |
17947 | | > hide #!11 models |
17948 | | |
17949 | | > close #13 |
17950 | | |
17951 | | > save W20_J4021_halfA_consensus_resampled.mrc #16 |
17952 | | |
17953 | | > save W20_J4021_halfB_consensus_resampled.mrc #14 |
17954 | | |
17955 | | > save W20_J4021_consensus_resampled.mrc #12 |
17956 | | |
17957 | | > close #6-8 |
17958 | | |
17959 | | > open "/Users/becca/Desktop/Postdoc/COPI notebooks and |
17960 | | > resources/Processing/W20_MM_cyto_COPI/W20_J4021_consensus_resampled.mrc" |
17961 | | |
17962 | | Opened W20_J4021_consensus_resampled.mrc as #6, grid size 240,240,240, pixel |
17963 | | 1.19, shown at level 0.259, step 1, values float32 |
17964 | | |
17965 | | > open "/Users/becca/Desktop/Postdoc/COPI notebooks and |
17966 | | > resources/Processing/W20_MM_cyto_COPI/W20_J4021_halfA_consensus_resampled.mrc" |
17967 | | > "/Users/becca/Desktop/Postdoc/COPI notebooks and |
17968 | | > resources/Processing/W20_MM_cyto_COPI/W20_J4021_halfB_consensus_resampled.mrc" |
17969 | | |
17970 | | Opened W20_J4021_halfA_consensus_resampled.mrc as #7.1, grid size 240,240,240, |
17971 | | pixel 1.19, shown at level 0.26, step 1, values float32 |
17972 | | Opened W20_J4021_halfB_consensus_resampled.mrc as #7.2, grid size 240,240,240, |
17973 | | pixel 1.19, shown at level 0.261, step 1, values float32 |
17974 | | |
17975 | | > rename #7 J4021_half_maps |
17976 | | |
17977 | | > combine #10 |
17978 | | |
17979 | | [Repeated 1 time(s)] |
17980 | | |
17981 | | > combine #11 |
17982 | | |
17983 | | > close #8 |
17984 | | |
17985 | | > close #9 |
17986 | | |
17987 | | > hide #!18 models |
17988 | | |
17989 | | > hide #!16 models |
17990 | | |
17991 | | > hide #!14 models |
17992 | | |
17993 | | > hide #!13 models |
17994 | | |
17995 | | > hide #!12 models |
17996 | | |
17997 | | > hide #!7 models |
17998 | | |
17999 | | > hide #!6 models |
18000 | | |
18001 | | > open "/Users/becca/Desktop/Postdoc/COPI notebooks and |
18002 | | > resources/Processing/W20_MM_cyto_COPI/W20_J4022_005_volume_map_bprime_local.mrc" |
18003 | | |
18004 | | Opened W20_J4022_005_volume_map_bprime_local.mrc as #8, grid size 240,240,240, |
18005 | | pixel 1.19, shown at level 0.256, step 1, values float32 |
18006 | | |
18007 | | > open "/Users/becca/Desktop/Postdoc/COPI notebooks and |
18008 | | > resources/Processing/W20_MM_cyto_COPI/cryosparc_P126_J4022_005_volume_map_half_A.mrc" |
18009 | | |
18010 | | Opened cryosparc_P126_J4022_005_volume_map_half_A.mrc as #9, grid size |
18011 | | 240,240,240, pixel 1.19, shown at level 0.259, step 1, values float32 |
18012 | | |
18013 | | > open "/Users/becca/Desktop/Postdoc/COPI notebooks and |
18014 | | > resources/Processing/W20_MM_cyto_COPI/cryosparc_P126_J4022_005_volume_map_half_B.mrc" |
18015 | | |
18016 | | Opened cryosparc_P126_J4022_005_volume_map_half_B.mrc as #20, grid size |
18017 | | 240,240,240, pixel 1.19, shown at level 0.257, step 1, values float32 |
18018 | | |
18019 | | > open "/Users/becca/Desktop/Postdoc/COPI notebooks and |
18020 | | > resources/Processing/W20_MM_cyto_COPI/cryosparc_P126_J4022_005_volume_map_half_B.mrc" |
18021 | | |
18022 | | Opened cryosparc_P126_J4022_005_volume_map_half_B.mrc as #22, grid size |
18023 | | 240,240,240, pixel 1.19, shown at level 0.257, step 1, values float32 |
18024 | | |
18025 | | > close #22 |
18026 | | |
18027 | | > close #20 |
18028 | | |
18029 | | > close #10 |
18030 | | |
18031 | | > close #11 |
18032 | | |
18033 | | > open "/Users/becca/Desktop/Postdoc/COPI notebooks and |
18034 | | > resources/Processing/W20_MM_cyto_COPI/cryosparc_P126_J4022_005_volume_map_half_B.mrc" |
18035 | | |
18036 | | Opened cryosparc_P126_J4022_005_volume_map_half_B.mrc as #10, grid size |
18037 | | 240,240,240, pixel 1.19, shown at level 0.257, step 1, values float32 |
18038 | | |
18039 | | > fitmap #8 inMap #1 |
18040 | | |
18041 | | Fit map W20_J4022_005_volume_map_bprime_local.mrc in map |
18042 | | W20_J3623_consensus_structure.mrc using 138200 points |
18043 | | correlation = 0.9195, correlation about mean = 0.2263, overlap = 1.667e+04 |
18044 | | steps = 64, shift = 0.329, angle = 0.376 degrees |
18045 | | |
18046 | | Position of W20_J4022_005_volume_map_bprime_local.mrc (#8) relative to |
18047 | | W20_J3623_consensus_structure.mrc (#1) coordinates: |
18048 | | Matrix rotation and translation |
18049 | | 0.99997966 0.00237299 0.00592048 -1.40324868 |
18050 | | -0.00238223 0.99999595 0.00155423 -0.17561136 |
18051 | | -0.00591677 -0.00156830 0.99998127 1.18884605 |
18052 | | Axis -0.23775685 0.90131776 -0.36207456 |
18053 | | Axis point 184.53246236 0.00000000 247.89429522 |
18054 | | Rotation angle (degrees) 0.37624341 |
18055 | | Shift along axis -0.25510056 |
18056 | | |
18057 | | |
18058 | | > fitmap #9 inMap #1 |
18059 | | |
18060 | | Fit map cryosparc_P126_J4022_005_volume_map_half_A.mrc in map |
18061 | | W20_J3623_consensus_structure.mrc using 138165 points |
18062 | | correlation = 0.9121, correlation about mean = 0.2433, overlap = 1.687e+04 |
18063 | | steps = 52, shift = 0.339, angle = 0.357 degrees |
18064 | | |
18065 | | Position of cryosparc_P126_J4022_005_volume_map_half_A.mrc (#9) relative to |
18066 | | W20_J3623_consensus_structure.mrc (#1) coordinates: |
18067 | | Matrix rotation and translation |
18068 | | 0.99998304 0.00160770 0.00559706 -1.23594018 |
18069 | | -0.00161999 0.99999628 0.00219278 -0.39387001 |
18070 | | -0.00559351 -0.00220181 0.99998193 1.22871953 |
18071 | | Axis -0.35302911 0.89896616 -0.25928803 |
18072 | | Axis point 207.94947596 0.00000000 236.35689026 |
18073 | | Rotation angle (degrees) 0.35661877 |
18074 | | Shift along axis -0.23634521 |
18075 | | |
18076 | | |
18077 | | > fitmap #10 inMap #1 |
18078 | | |
18079 | | Fit map cryosparc_P126_J4022_005_volume_map_half_B.mrc in map |
18080 | | W20_J3623_consensus_structure.mrc using 138076 points |
18081 | | correlation = 0.9128, correlation about mean = 0.2508, overlap = 1.688e+04 |
18082 | | steps = 64, shift = 0.335, angle = 0.335 degrees |
18083 | | |
18084 | | Position of cryosparc_P126_J4022_005_volume_map_half_B.mrc (#10) relative to |
18085 | | W20_J3623_consensus_structure.mrc (#1) coordinates: |
18086 | | Matrix rotation and translation |
18087 | | 0.99998354 0.00229208 0.00525924 -1.29178101 |
18088 | | -0.00229810 0.99999671 0.00113989 -0.13676617 |
18089 | | -0.00525661 -0.00115196 0.99998552 0.99455043 |
18090 | | Axis -0.19587427 0.89873969 -0.39230121 |
18091 | | Axis point 169.08755660 -0.00000000 255.83594172 |
18092 | | Rotation angle (degrees) 0.33520093 |
18093 | | Shift along axis -0.26005386 |
18094 | | |
18095 | | |
18096 | | > volume resample #8 #9 #10 onGrid #1 |
18097 | | |
18098 | | Opened W20_J4022_005_volume_map_bprime_local.mrc resampled as #11, grid size |
18099 | | 240,240,240, pixel 1.19, shown at step 1, values float32 |
18100 | | Opened cryosparc_P126_J4022_005_volume_map_half_A.mrc resampled as #20, grid |
18101 | | size 240,240,240, pixel 1.19, shown at step 1, values float32 |
18102 | | Opened cryosparc_P126_J4022_005_volume_map_half_B.mrc resampled as #22, grid |
18103 | | size 240,240,240, pixel 1.19, shown at step 1, values float32 |
18104 | | |
18105 | | > save W20_J4022_halfB_consensus_resampled.mrc #22 |
18106 | | |
18107 | | > save W20_J4022_half1_consensus_resampled.mrc #20 |
18108 | | |
18109 | | > save W20_J4022_halfA_consensus_resampled.mrc #20 |
18110 | | |
18111 | | > save W20_J4022_consensus_resampled.mrc #11 |
18112 | | |
18113 | | > close #8-10 |
18114 | | |
18115 | | > open "/Users/becca/Desktop/Postdoc/COPI notebooks and |
18116 | | > resources/Processing/W20_MM_cyto_COPI/W20_J4022_consensus_resampled.mrc" |
18117 | | |
18118 | | Opened W20_J4022_consensus_resampled.mrc as #8, grid size 240,240,240, pixel |
18119 | | 1.19, shown at level 0.256, step 1, values float32 |
18120 | | |
18121 | | > open "/Users/becca/Desktop/Postdoc/COPI notebooks and |
18122 | | > resources/Processing/W20_MM_cyto_COPI/W20_J4022_halfA_consensus_resampled.mrc" |
18123 | | > "/Users/becca/Desktop/Postdoc/COPI notebooks and |
18124 | | > resources/Processing/W20_MM_cyto_COPI/W20_J4022_halfB_consensus_resampled.mrc" |
18125 | | |
18126 | | Opened W20_J4022_halfA_consensus_resampled.mrc as #9.1, grid size 240,240,240, |
18127 | | pixel 1.19, shown at level 0.257, step 1, values float32 |
18128 | | Opened W20_J4022_halfB_consensus_resampled.mrc as #9.2, grid size 240,240,240, |
18129 | | pixel 1.19, shown at level 0.255, step 1, values float32 |
18130 | | |
18131 | | > rename #9 J4022_half_maps |
18132 | | |
18133 | | > combine #13 |
18134 | | |
18135 | | [Repeated 1 time(s)] |
18136 | | |
18137 | | > combine #18 |
18138 | | |
18139 | | > hide #!10 models |
18140 | | |
18141 | | > close #10-17 |
18142 | | |
18143 | | > hide #!9 models |
18144 | | |
18145 | | > hide #!8 models |
18146 | | |
18147 | | > show #!25 models |
18148 | | |
18149 | | > hide #!24 models |
18150 | | |
18151 | | > hide #!26 models |
18152 | | |
18153 | | > hide #!25 models |
18154 | | |
18155 | | > hide #!22 models |
18156 | | |
18157 | | > hide #!20 models |
18158 | | |
18159 | | > open "/Users/becca/Desktop/Postdoc/COPI notebooks and |
18160 | | > resources/Processing/W20_MM_cyto_COPI/W20_J4023_003_volume_map_bprime_local.mrc" |
18161 | | |
18162 | | Opened W20_J4023_003_volume_map_bprime_local.mrc as #10, grid size |
18163 | | 240,240,240, pixel 1.19, shown at level 0.26, step 1, values float32 |
18164 | | |
18165 | | > open "/Users/becca/Desktop/Postdoc/COPI notebooks and |
18166 | | > resources/Processing/W20_MM_cyto_COPI/cryosparc_P126_J4023_003_volume_map_half_A.mrc" |
18167 | | |
18168 | | Opened cryosparc_P126_J4023_003_volume_map_half_A.mrc as #11, grid size |
18169 | | 240,240,240, pixel 1.19, shown at level 0.26, step 1, values float32 |
18170 | | |
18171 | | > open "/Users/becca/Desktop/Postdoc/COPI notebooks and |
18172 | | > resources/Processing/W20_MM_cyto_COPI/cryosparc_P126_J4023_003_volume_map_half_B.mrc" |
18173 | | |
18174 | | Opened cryosparc_P126_J4023_003_volume_map_half_B.mrc as #12, grid size |
18175 | | 240,240,240, pixel 1.19, shown at level 0.261, step 1, values float32 |
18176 | | |
18177 | | > fitmap #10 inMap #1 |
18178 | | |
18179 | | Fit map W20_J4023_003_volume_map_bprime_local.mrc in map |
18180 | | W20_J3623_consensus_structure.mrc using 138098 points |
18181 | | correlation = 0.9217, correlation about mean = 0.2228, overlap = 1.65e+04 |
18182 | | steps = 52, shift = 0.377, angle = 0.32 degrees |
18183 | | |
18184 | | Position of W20_J4023_003_volume_map_bprime_local.mrc (#10) relative to |
18185 | | W20_J3623_consensus_structure.mrc (#1) coordinates: |
18186 | | Matrix rotation and translation |
18187 | | 0.99998445 0.00551741 0.00081396 -1.06075152 |
18188 | | -0.00551761 0.99998475 0.00023719 0.41218042 |
18189 | | -0.00081264 -0.00024168 0.99999964 0.27797318 |
18190 | | Axis -0.04289152 0.14569399 -0.98839950 |
18191 | | Axis point 78.63485140 193.79218176 0.00000000 |
18192 | | Rotation angle (degrees) 0.31984208 |
18193 | | Shift along axis -0.16919909 |
18194 | | |
18195 | | |
18196 | | > fitmap #11 inMap #1 |
18197 | | |
18198 | | Fit map cryosparc_P126_J4023_003_volume_map_half_A.mrc in map |
18199 | | W20_J3623_consensus_structure.mrc using 138113 points |
18200 | | correlation = 0.9179, correlation about mean = 0.2457, overlap = 1.663e+04 |
18201 | | steps = 52, shift = 0.377, angle = 0.301 degrees |
18202 | | |
18203 | | Position of cryosparc_P126_J4023_003_volume_map_half_A.mrc (#11) relative to |
18204 | | W20_J3623_consensus_structure.mrc (#1) coordinates: |
18205 | | Matrix rotation and translation |
18206 | | 0.99998625 0.00523529 0.00031389 -0.97109463 |
18207 | | -0.00523523 0.99998627 -0.00021220 0.44237207 |
18208 | | -0.00031500 0.00021056 0.99999993 0.12645487 |
18209 | | Axis 0.04027075 0.05990654 -0.99739133 |
18210 | | Axis point 85.60249694 184.64779847 0.00000000 |
18211 | | Rotation angle (degrees) 0.30074416 |
18212 | | Shift along axis -0.13873071 |
18213 | | |
18214 | | |
18215 | | > fitmap #11 inMap #1 |
18216 | | |
18217 | | Fit map cryosparc_P126_J4023_003_volume_map_half_A.mrc in map |
18218 | | W20_J3623_consensus_structure.mrc using 138113 points |
18219 | | correlation = 0.9179, correlation about mean = 0.2458, overlap = 1.664e+04 |
18220 | | steps = 28, shift = 0.0171, angle = 0.0164 degrees |
18221 | | |
18222 | | Position of cryosparc_P126_J4023_003_volume_map_half_A.mrc (#11) relative to |
18223 | | W20_J3623_consensus_structure.mrc (#1) coordinates: |
18224 | | Matrix rotation and translation |
18225 | | 0.99998502 0.00545441 0.00046410 -1.02505042 |
18226 | | -0.00545426 0.99998508 -0.00031696 0.50618634 |
18227 | | -0.00046583 0.00031443 0.99999984 0.13601178 |
18228 | | Axis 0.05757496 0.08479798 -0.99473334 |
18229 | | Axis point 94.64872752 186.59259606 0.00000000 |
18230 | | Rotation angle (degrees) 0.31416658 |
18231 | | Shift along axis -0.15138911 |
18232 | | |
18233 | | |
18234 | | > volume resample #11 #12 #10 onGrid #1 |
18235 | | |
18236 | | Opened cryosparc_P126_J4023_003_volume_map_half_A.mrc resampled as #13, grid |
18237 | | size 240,240,240, pixel 1.19, shown at step 1, values float32 |
18238 | | Opened cryosparc_P126_J4023_003_volume_map_half_B.mrc resampled as #14, grid |
18239 | | size 240,240,240, pixel 1.19, shown at step 1, values float32 |
18240 | | Opened W20_J4023_003_volume_map_bprime_local.mrc resampled as #15, grid size |
18241 | | 240,240,240, pixel 1.19, shown at step 1, values float32 |
18242 | | |
18243 | | > save W20_J4022_halfA_consensus_resampled.mrc #13 |
18244 | | |
18245 | | > save W20_J4022_halfB_consensus_resampled.mrc #14 |
18246 | | |
18247 | | > save W20_J4022_consensus_resampled.mrc #15 |
18248 | | |
18249 | | > close #10-15 |
18250 | | |
18251 | | > open "/Users/becca/Desktop/Postdoc/COPI notebooks and |
18252 | | > resources/Processing/W20_MM_cyto_COPI/W20_J4022_consensus_resampled.mrc" |
18253 | | |
18254 | | Opened W20_J4022_consensus_resampled.mrc as #10, grid size 240,240,240, pixel |
18255 | | 1.19, shown at level 0.256, step 1, values float32 |
18256 | | |
18257 | | > show #!8 models |
18258 | | |
18259 | | > hide #!10 models |
18260 | | |
18261 | | > show #!10 models |
18262 | | |
18263 | | > hide #!10 models |
18264 | | |
18265 | | > show #!10 models |
18266 | | |
18267 | | > hide #!10 models |
18268 | | |
18269 | | > show #!10 models |
18270 | | |
18271 | | > hide #!10 models |
18272 | | |
18273 | | > show #!10 models |
18274 | | |
18275 | | > hide #!10 models |
18276 | | |
18277 | | > close #8-10 |
18278 | | |
18279 | | > open "/Users/becca/Desktop/Postdoc/COPI notebooks and |
18280 | | > resources/Processing/W20_MM_cyto_COPI/W20_J4022_005_volume_map_bprime_local.mrc" |
18281 | | |
18282 | | Opened W20_J4022_005_volume_map_bprime_local.mrc as #8, grid size 240,240,240, |
18283 | | pixel 1.19, shown at level 0.256, step 1, values float32 |
18284 | | |
18285 | | > open "/Users/becca/Desktop/Postdoc/COPI notebooks and |
18286 | | > resources/Processing/W20_MM_cyto_COPI/cryosparc_P126_J4022_005_volume_map_half_A.mrc" |
18287 | | |
18288 | | Opened cryosparc_P126_J4022_005_volume_map_half_A.mrc as #9, grid size |
18289 | | 240,240,240, pixel 1.19, shown at level 0.259, step 1, values float32 |
18290 | | |
18291 | | > open "/Users/becca/Desktop/Postdoc/COPI notebooks and |
18292 | | > resources/Processing/W20_MM_cyto_COPI/cryosparc_P126_J4022_005_volume_map_half_B.mrc" |
18293 | | |
18294 | | Opened cryosparc_P126_J4022_005_volume_map_half_B.mrc as #10, grid size |
18295 | | 240,240,240, pixel 1.19, shown at level 0.257, step 1, values float32 |
18296 | | |
18297 | | > fitmap #8 inMap #1 |
18298 | | |
18299 | | Fit map W20_J4022_005_volume_map_bprime_local.mrc in map |
18300 | | W20_J3623_consensus_structure.mrc using 138200 points |
18301 | | correlation = 0.9195, correlation about mean = 0.2263, overlap = 1.667e+04 |
18302 | | steps = 64, shift = 0.329, angle = 0.376 degrees |
18303 | | |
18304 | | Position of W20_J4022_005_volume_map_bprime_local.mrc (#8) relative to |
18305 | | W20_J3623_consensus_structure.mrc (#1) coordinates: |
18306 | | Matrix rotation and translation |
18307 | | 0.99997966 0.00237299 0.00592048 -1.40324868 |
18308 | | -0.00238223 0.99999595 0.00155423 -0.17561136 |
18309 | | -0.00591677 -0.00156830 0.99998127 1.18884605 |
18310 | | Axis -0.23775685 0.90131776 -0.36207456 |
18311 | | Axis point 184.53246236 0.00000000 247.89429522 |
18312 | | Rotation angle (degrees) 0.37624341 |
18313 | | Shift along axis -0.25510056 |
18314 | | |
18315 | | |
18316 | | > fitmap #9 inMap #1 |
18317 | | |
18318 | | Fit map cryosparc_P126_J4022_005_volume_map_half_A.mrc in map |
18319 | | W20_J3623_consensus_structure.mrc using 138165 points |
18320 | | correlation = 0.9121, correlation about mean = 0.2433, overlap = 1.687e+04 |
18321 | | steps = 52, shift = 0.339, angle = 0.357 degrees |
18322 | | |
18323 | | Position of cryosparc_P126_J4022_005_volume_map_half_A.mrc (#9) relative to |
18324 | | W20_J3623_consensus_structure.mrc (#1) coordinates: |
18325 | | Matrix rotation and translation |
18326 | | 0.99998304 0.00160770 0.00559706 -1.23594018 |
18327 | | -0.00161999 0.99999628 0.00219278 -0.39387001 |
18328 | | -0.00559351 -0.00220181 0.99998193 1.22871953 |
18329 | | Axis -0.35302911 0.89896616 -0.25928803 |
18330 | | Axis point 207.94947596 0.00000000 236.35689026 |
18331 | | Rotation angle (degrees) 0.35661877 |
18332 | | Shift along axis -0.23634521 |
18333 | | |
18334 | | |
18335 | | > fitmap #10 inMap #1 |
18336 | | |
18337 | | Fit map cryosparc_P126_J4022_005_volume_map_half_B.mrc in map |
18338 | | W20_J3623_consensus_structure.mrc using 138076 points |
18339 | | correlation = 0.9128, correlation about mean = 0.2508, overlap = 1.688e+04 |
18340 | | steps = 64, shift = 0.335, angle = 0.335 degrees |
18341 | | |
18342 | | Position of cryosparc_P126_J4022_005_volume_map_half_B.mrc (#10) relative to |
18343 | | W20_J3623_consensus_structure.mrc (#1) coordinates: |
18344 | | Matrix rotation and translation |
18345 | | 0.99998354 0.00229208 0.00525924 -1.29178101 |
18346 | | -0.00229810 0.99999671 0.00113989 -0.13676617 |
18347 | | -0.00525661 -0.00115196 0.99998552 0.99455043 |
18348 | | Axis -0.19587427 0.89873969 -0.39230121 |
18349 | | Axis point 169.08755660 -0.00000000 255.83594172 |
18350 | | Rotation angle (degrees) 0.33520093 |
18351 | | Shift along axis -0.26005386 |
18352 | | |
18353 | | |
18354 | | > volume resample #8 #9 #10 onGrid #1 |
18355 | | |
18356 | | Opened W20_J4022_005_volume_map_bprime_local.mrc resampled as #11, grid size |
18357 | | 240,240,240, pixel 1.19, shown at step 1, values float32 |
18358 | | Opened cryosparc_P126_J4022_005_volume_map_half_A.mrc resampled as #12, grid |
18359 | | size 240,240,240, pixel 1.19, shown at step 1, values float32 |
18360 | | Opened cryosparc_P126_J4022_005_volume_map_half_B.mrc resampled as #13, grid |
18361 | | size 240,240,240, pixel 1.19, shown at step 1, values float32 |
18362 | | |
18363 | | > save W20_J4022_halfA_consensus_resampled.mrc #12 |
18364 | | |
18365 | | > save W20_J4022_halfb_consensus_resampled.mrc #12 |
18366 | | |
18367 | | > save W20_J4022_halfB_consensus_resampled.mrc #12 |
18368 | | |
18369 | | > save W20_J4022_halfB_consensus_resampled.mrc #13 |
18370 | | |
18371 | | > save W20_J4022_halfA_consensus_resampled.mrc #12 |
18372 | | |
18373 | | > save W20_J4022_consensus_resampled.mrc #11 |
18374 | | |
18375 | | > close #8-10 |
18376 | | |
18377 | | > open "/Users/becca/Desktop/Postdoc/COPI notebooks and |
18378 | | > resources/Processing/W20_MM_cyto_COPI/W20_J4022_consensus_resampled.mrc" |
18379 | | |
18380 | | Opened W20_J4022_consensus_resampled.mrc as #8, grid size 240,240,240, pixel |
18381 | | 1.19, shown at level 0.256, step 1, values float32 |
18382 | | |
18383 | | > open "/Users/becca/Desktop/Postdoc/COPI notebooks and |
18384 | | > resources/Processing/W20_MM_cyto_COPI/W20_J4022_halfA_consensus_resampled.mrc" |
18385 | | > "/Users/becca/Desktop/Postdoc/COPI notebooks and |
18386 | | > resources/Processing/W20_MM_cyto_COPI/W20_J4022_halfB_consensus_resampled.mrc" |
18387 | | |
18388 | | Opened W20_J4022_halfA_consensus_resampled.mrc as #9.1, grid size 240,240,240, |
18389 | | pixel 1.19, shown at level 0.257, step 1, values float32 |
18390 | | Opened W20_J4022_halfB_consensus_resampled.mrc as #9.2, grid size 240,240,240, |
18391 | | pixel 1.19, shown at level 0.255, step 1, values float32 |
18392 | | |
18393 | | > rename #9 J4022_half_maps |
18394 | | |
18395 | | > close #11-13 |
18396 | | |
18397 | | > hide #!9 models |
18398 | | |
18399 | | > hide #!8 models |
18400 | | |
18401 | | > open "/Users/becca/Desktop/Postdoc/COPI notebooks and |
18402 | | > resources/Processing/W20_MM_cyto_COPI/cryosparc_P126_J4023_003_volume_map_half_A.mrc" |
18403 | | |
18404 | | Opened cryosparc_P126_J4023_003_volume_map_half_A.mrc as #10, grid size |
18405 | | 240,240,240, pixel 1.19, shown at level 0.26, step 1, values float32 |
18406 | | |
18407 | | > open "/Users/becca/Desktop/Postdoc/COPI notebooks and |
18408 | | > resources/Processing/W20_MM_cyto_COPI/cryosparc_P126_J4023_003_volume_map_half_B.mrc" |
18409 | | |
18410 | | Opened cryosparc_P126_J4023_003_volume_map_half_B.mrc as #11, grid size |
18411 | | 240,240,240, pixel 1.19, shown at level 0.261, step 1, values float32 |
18412 | | |
18413 | | > open "/Users/becca/Desktop/Postdoc/COPI notebooks and |
18414 | | > resources/Processing/W20_MM_cyto_COPI/W20_J4023_003_volume_map_bprime_local.mrc" |
18415 | | |
18416 | | Opened W20_J4023_003_volume_map_bprime_local.mrc as #12, grid size |
18417 | | 240,240,240, pixel 1.19, shown at level 0.26, step 1, values float32 |
18418 | | |
18419 | | > fitmap #10 inMap #1 |
18420 | | |
18421 | | Fit map cryosparc_P126_J4023_003_volume_map_half_A.mrc in map |
18422 | | W20_J3623_consensus_structure.mrc using 138113 points |
18423 | | correlation = 0.9179, correlation about mean = 0.2457, overlap = 1.663e+04 |
18424 | | steps = 52, shift = 0.377, angle = 0.301 degrees |
18425 | | |
18426 | | Position of cryosparc_P126_J4023_003_volume_map_half_A.mrc (#10) relative to |
18427 | | W20_J3623_consensus_structure.mrc (#1) coordinates: |
18428 | | Matrix rotation and translation |
18429 | | 0.99998625 0.00523529 0.00031389 -0.97109463 |
18430 | | -0.00523523 0.99998627 -0.00021220 0.44237207 |
18431 | | -0.00031500 0.00021056 0.99999993 0.12645487 |
18432 | | Axis 0.04027075 0.05990654 -0.99739133 |
18433 | | Axis point 85.60249694 184.64779847 0.00000000 |
18434 | | Rotation angle (degrees) 0.30074416 |
18435 | | Shift along axis -0.13873071 |
18436 | | |
18437 | | |
18438 | | > fitmap #11 inMap #1 |
18439 | | |
18440 | | Fit map cryosparc_P126_J4023_003_volume_map_half_B.mrc in map |
18441 | | W20_J3623_consensus_structure.mrc using 138029 points |
18442 | | correlation = 0.9185, correlation about mean = 0.2483, overlap = 1.668e+04 |
18443 | | steps = 68, shift = 0.416, angle = 0.319 degrees |
18444 | | |
18445 | | Position of cryosparc_P126_J4023_003_volume_map_half_B.mrc (#11) relative to |
18446 | | W20_J3623_consensus_structure.mrc (#1) coordinates: |
18447 | | Matrix rotation and translation |
18448 | | 0.99998492 0.00536357 0.00117882 -1.09568335 |
18449 | | -0.00536466 0.99998519 0.00091977 0.25675329 |
18450 | | -0.00117387 -0.00092608 0.99999888 0.47324489 |
18451 | | Axis -0.16573766 0.21124575 -0.96327891 |
18452 | | Axis point 55.94119463 211.23890617 0.00000000 |
18453 | | Rotation angle (degrees) 0.31905878 |
18454 | | Shift along axis -0.22003278 |
18455 | | |
18456 | | |
18457 | | > fitmap #12 inMap #1 |
18458 | | |
18459 | | Fit map W20_J4023_003_volume_map_bprime_local.mrc in map |
18460 | | W20_J3623_consensus_structure.mrc using 138098 points |
18461 | | correlation = 0.9217, correlation about mean = 0.2228, overlap = 1.65e+04 |
18462 | | steps = 52, shift = 0.377, angle = 0.32 degrees |
18463 | | |
18464 | | Position of W20_J4023_003_volume_map_bprime_local.mrc (#12) relative to |
18465 | | W20_J3623_consensus_structure.mrc (#1) coordinates: |
18466 | | Matrix rotation and translation |
18467 | | 0.99998445 0.00551741 0.00081396 -1.06075152 |
18468 | | -0.00551761 0.99998475 0.00023719 0.41218042 |
18469 | | -0.00081264 -0.00024168 0.99999964 0.27797318 |
18470 | | Axis -0.04289152 0.14569399 -0.98839950 |
18471 | | Axis point 78.63485140 193.79218176 0.00000000 |
18472 | | Rotation angle (degrees) 0.31984208 |
18473 | | Shift along axis -0.16919909 |
18474 | | |
18475 | | |
18476 | | > volume resample #11 #12 #10 onGrid #1 |
18477 | | |
18478 | | Opened cryosparc_P126_J4023_003_volume_map_half_B.mrc resampled as #13, grid |
18479 | | size 240,240,240, pixel 1.19, shown at step 1, values float32 |
18480 | | Opened W20_J4023_003_volume_map_bprime_local.mrc resampled as #14, grid size |
18481 | | 240,240,240, pixel 1.19, shown at step 1, values float32 |
18482 | | Opened cryosparc_P126_J4023_003_volume_map_half_A.mrc resampled as #15, grid |
18483 | | size 240,240,240, pixel 1.19, shown at step 1, values float32 |
18484 | | |
18485 | | > save W20_J4023_halfA_consensus_resampled.mrc #15 |
18486 | | |
18487 | | > save W20_J4023_halfB_consensus_resampled.mrc #13 |
18488 | | |
18489 | | > save W20_J4023_consensus_resampled.mrc #14 |
18490 | | |
18491 | | > close #10-12 |
18492 | | |
18493 | | > open "/Users/becca/Desktop/Postdoc/COPI notebooks and |
18494 | | > resources/Processing/W20_MM_cyto_COPI/W20_J4023_consensus_resampled.mrc" |
18495 | | |
18496 | | Opened W20_J4023_consensus_resampled.mrc as #10, grid size 240,240,240, pixel |
18497 | | 1.19, shown at level 0.259, step 1, values float32 |
18498 | | |
18499 | | > open "/Users/becca/Desktop/Postdoc/COPI notebooks and |
18500 | | > resources/Processing/W20_MM_cyto_COPI/W20_J4023_halfA_consensus_resampled.mrc" |
18501 | | > "/Users/becca/Desktop/Postdoc/COPI notebooks and |
18502 | | > resources/Processing/W20_MM_cyto_COPI/W20_J4023_halfB_consensus_resampled.mrc" |
18503 | | |
18504 | | Opened W20_J4023_halfA_consensus_resampled.mrc as #11.1, grid size |
18505 | | 240,240,240, pixel 1.19, shown at level 0.258, step 1, values float32 |
18506 | | Opened W20_J4023_halfB_consensus_resampled.mrc as #11.2, grid size |
18507 | | 240,240,240, pixel 1.19, shown at level 0.259, step 1, values float32 |
18508 | | |
18509 | | > rename #11 J4023_half_maps |
18510 | | |
18511 | | > close #13-15 |
18512 | | |
18513 | | > open "/Users/becca/Desktop/Postdoc/COPI notebooks and |
18514 | | > resources/Processing/W20_MM_cyto_COPI/cryosparc_P126_J4024_005_volume_map_half_B.mrc" |
18515 | | |
18516 | | Opened cryosparc_P126_J4024_005_volume_map_half_B.mrc as #12, grid size |
18517 | | 240,240,240, pixel 1.19, shown at level 0.258, step 1, values float32 |
18518 | | |
18519 | | > open "/Users/becca/Desktop/Postdoc/COPI notebooks and |
18520 | | > resources/Processing/W20_MM_cyto_COPI/cryosparc_P126_J4024_005_volume_map_half_A.mrc" |
18521 | | |
18522 | | Opened cryosparc_P126_J4024_005_volume_map_half_A.mrc as #13, grid size |
18523 | | 240,240,240, pixel 1.19, shown at level 0.259, step 1, values float32 |
18524 | | |
18525 | | > open "/Users/becca/Desktop/Postdoc/COPI notebooks and |
18526 | | > resources/Processing/W20_MM_cyto_COPI/W20_J4024_005_volume_map_bprime_local.mrc" |
18527 | | |
18528 | | Opened W20_J4024_005_volume_map_bprime_local.mrc as #14, grid size |
18529 | | 240,240,240, pixel 1.19, shown at level 0.256, step 1, values float32 |
18530 | | |
18531 | | > hide #!10 models |
18532 | | |
18533 | | > hide #!11 models |
18534 | | |
18535 | | > fitmap #12 inMap #1 |
18536 | | |
18537 | | Fit map cryosparc_P126_J4024_005_volume_map_half_B.mrc in map |
18538 | | W20_J3623_consensus_structure.mrc using 138074 points |
18539 | | correlation = 0.9152, correlation about mean = 0.3069, overlap = 1.694e+04 |
18540 | | steps = 48, shift = 0.275, angle = 0.182 degrees |
18541 | | |
18542 | | Position of cryosparc_P126_J4024_005_volume_map_half_B.mrc (#12) relative to |
18543 | | W20_J3623_consensus_structure.mrc (#1) coordinates: |
18544 | | Matrix rotation and translation |
18545 | | 0.99999497 0.00227004 0.00221345 -0.56219185 |
18546 | | -0.00226948 0.99999739 -0.00025704 0.14204421 |
18547 | | -0.00221402 0.00025201 0.99999752 0.19043665 |
18548 | | Axis 0.08002006 0.69597786 -0.71359065 |
18549 | | Axis point 87.46071279 244.95972740 0.00000000 |
18550 | | Rotation angle (degrees) 0.18224416 |
18551 | | Shift along axis -0.08202081 |
18552 | | |
18553 | | |
18554 | | > fitmap #13 inMap #1 |
18555 | | |
18556 | | Fit map cryosparc_P126_J4024_005_volume_map_half_A.mrc in map |
18557 | | W20_J3623_consensus_structure.mrc using 138160 points |
18558 | | correlation = 0.9177, correlation about mean = 0.3098, overlap = 1.706e+04 |
18559 | | steps = 52, shift = 0.291, angle = 0.229 degrees |
18560 | | |
18561 | | Position of cryosparc_P126_J4024_005_volume_map_half_A.mrc (#13) relative to |
18562 | | W20_J3623_consensus_structure.mrc (#1) coordinates: |
18563 | | Matrix rotation and translation |
18564 | | 0.99999333 0.00148868 0.00333575 -0.58679763 |
18565 | | -0.00149415 0.99999754 0.00163794 -0.27610320 |
18566 | | -0.00333331 -0.00164291 0.99999309 0.70324776 |
18567 | | Axis -0.40966600 0.83273727 -0.37245458 |
18568 | | Axis point 182.44969494 0.00000000 207.15832332 |
18569 | | Rotation angle (degrees) 0.22943002 |
18570 | | Shift along axis -0.25145824 |
18571 | | |
18572 | | |
18573 | | > fitmap #14 inMap #1 |
18574 | | |
18575 | | Fit map W20_J4024_005_volume_map_bprime_local.mrc in map |
18576 | | W20_J3623_consensus_structure.mrc using 138193 points |
18577 | | correlation = 0.9249, correlation about mean = 0.3066, overlap = 1.679e+04 |
18578 | | steps = 64, shift = 0.289, angle = 0.207 degrees |
18579 | | |
18580 | | Position of W20_J4024_005_volume_map_bprime_local.mrc (#14) relative to |
18581 | | W20_J3623_consensus_structure.mrc (#1) coordinates: |
18582 | | Matrix rotation and translation |
18583 | | 0.99999384 0.00210499 0.00280839 -0.61107104 |
18584 | | -0.00210740 0.99999741 0.00085537 -0.06363233 |
18585 | | -0.00280658 -0.00086128 0.99999569 0.47576775 |
18586 | | Axis -0.23755676 0.77702082 -0.58292833 |
18587 | | Axis point 131.43847496 0.00000000 233.23904124 |
18588 | | Rotation angle (degrees) 0.20701789 |
18589 | | Shift along axis -0.18161809 |
18590 | | |
18591 | | |
18592 | | > volume resample #13 #12 #14 onGrid #1 |
18593 | | |
18594 | | Opened cryosparc_P126_J4024_005_volume_map_half_A.mrc resampled as #15, grid |
18595 | | size 240,240,240, pixel 1.19, shown at step 1, values float32 |
18596 | | Opened cryosparc_P126_J4024_005_volume_map_half_B.mrc resampled as #16, grid |
18597 | | size 240,240,240, pixel 1.19, shown at step 1, values float32 |
18598 | | Opened W20_J4024_005_volume_map_bprime_local.mrc resampled as #17, grid size |
18599 | | 240,240,240, pixel 1.19, shown at step 1, values float32 |
18600 | | |
18601 | | > save W20_J4024_halfA_consensus_resampled.mrc #15 |
18602 | | |
18603 | | > save W20_J4024_halfB_consensus_resampled.mrc #16 |
18604 | | |
18605 | | > save W20_J4024_consensus_resampled.mrc #17 |
18606 | | |
18607 | | > combine #18 |
18608 | | |
18609 | | > hide #!28 models |
18610 | | |
18611 | | > close #12-14 |
18612 | | |
18613 | | > open "/Users/becca/Desktop/Postdoc/COPI notebooks and |
18614 | | > resources/Processing/W20_MM_cyto_COPI/W20_J4024_consensus_resampled.mrc" |
18615 | | |
18616 | | Opened W20_J4024_consensus_resampled.mrc as #12, grid size 240,240,240, pixel |
18617 | | 1.19, shown at level 0.256, step 1, values float32 |
18618 | | |
18619 | | > open "/Users/becca/Desktop/Postdoc/COPI notebooks and |
18620 | | > resources/Processing/W20_MM_cyto_COPI/W20_J4024_halfA_consensus_resampled.mrc" |
18621 | | > "/Users/becca/Desktop/Postdoc/COPI notebooks and |
18622 | | > resources/Processing/W20_MM_cyto_COPI/W20_J4024_halfB_consensus_resampled.mrc" |
18623 | | |
18624 | | Opened W20_J4024_halfA_consensus_resampled.mrc as #13.1, grid size |
18625 | | 240,240,240, pixel 1.19, shown at level 0.257, step 1, values float32 |
18626 | | Opened W20_J4024_halfB_consensus_resampled.mrc as #13.2, grid size |
18627 | | 240,240,240, pixel 1.19, shown at level 0.257, step 1, values float32 |
18628 | | |
18629 | | > rename #13 J4024_half_maps |
18630 | | |
18631 | | > hide #!13 models |
18632 | | |
18633 | | > hide #!12 models |
18634 | | |
18635 | | > close #15-17 |
18636 | | |
18637 | | > open "/Users/becca/Desktop/Postdoc/COPI notebooks and |
18638 | | > resources/Processing/W20_MM_cyto_COPI/W20_J4025_004_volume_map_bprime_local.mrc" |
18639 | | |
18640 | | Opened W20_J4025_004_volume_map_bprime_local.mrc as #14, grid size |
18641 | | 240,240,240, pixel 1.19, shown at level 0.261, step 1, values float32 |
18642 | | |
18643 | | > open "/Users/becca/Desktop/Postdoc/COPI notebooks and |
18644 | | > resources/Processing/W20_MM_cyto_COPI/cryosparc_P126_J4025_004_volume_map_half_A.mrc" |
18645 | | |
18646 | | Opened cryosparc_P126_J4025_004_volume_map_half_A.mrc as #15, grid size |
18647 | | 240,240,240, pixel 1.19, shown at level 0.261, step 1, values float32 |
18648 | | |
18649 | | > open "/Users/becca/Desktop/Postdoc/COPI notebooks and |
18650 | | > resources/Processing/W20_MM_cyto_COPI/cryosparc_P126_J4025_004_volume_map_half_B.mrc" |
18651 | | |
18652 | | Opened cryosparc_P126_J4025_004_volume_map_half_B.mrc as #16, grid size |
18653 | | 240,240,240, pixel 1.19, shown at level 0.262, step 1, values float32 |
18654 | | |
18655 | | > fitmap #14 inMap #1 |
18656 | | |
18657 | | Fit map W20_J4025_004_volume_map_bprime_local.mrc in map |
18658 | | W20_J3623_consensus_structure.mrc using 138084 points |
18659 | | correlation = 0.9112, correlation about mean = 0.1801, overlap = 1.617e+04 |
18660 | | steps = 56, shift = 0.729, angle = 0.309 degrees |
18661 | | |
18662 | | Position of W20_J4025_004_volume_map_bprime_local.mrc (#14) relative to |
18663 | | W20_J3623_consensus_structure.mrc (#1) coordinates: |
18664 | | Matrix rotation and translation |
18665 | | 0.99999126 0.00417664 -0.00019252 -0.45232897 |
18666 | | -0.00417727 0.99998545 -0.00341415 0.60739960 |
18667 | | 0.00017826 0.00341492 0.99999415 -0.09374688 |
18668 | | Axis 0.63253451 -0.03434313 -0.77377041 |
18669 | | Axis point 143.22381791 73.09529688 0.00000000 |
18670 | | Rotation angle (degrees) 0.30929457 |
18671 | | Shift along axis -0.23443512 |
18672 | | |
18673 | | |
18674 | | > fitmap #15 inMap #1 |
18675 | | |
18676 | | Fit map cryosparc_P126_J4025_004_volume_map_half_A.mrc in map |
18677 | | W20_J3623_consensus_structure.mrc using 138098 points |
18678 | | correlation = 0.9052, correlation about mean = 0.2004, overlap = 1.632e+04 |
18679 | | steps = 56, shift = 0.719, angle = 0.323 degrees |
18680 | | |
18681 | | Position of cryosparc_P126_J4025_004_volume_map_half_A.mrc (#15) relative to |
18682 | | W20_J3623_consensus_structure.mrc (#1) coordinates: |
18683 | | Matrix rotation and translation |
18684 | | 0.99998806 0.00478731 -0.00097621 -0.44930386 |
18685 | | -0.00479005 0.99998454 -0.00282388 0.63846920 |
18686 | | 0.00096268 0.00282852 0.99999554 -0.08426557 |
18687 | | Axis 0.50071333 -0.17175476 -0.84840230 |
18688 | | Axis point 123.64037299 66.63895829 0.00000000 |
18689 | | Rotation angle (degrees) 0.32339898 |
18690 | | Shift along axis -0.26314146 |
18691 | | |
18692 | | |
18693 | | > fitmap #16 inMap #1 |
18694 | | |
18695 | | Fit map cryosparc_P126_J4025_004_volume_map_half_B.mrc in map |
18696 | | W20_J3623_consensus_structure.mrc using 138173 points |
18697 | | correlation = 0.9079, correlation about mean = 0.2131, overlap = 1.642e+04 |
18698 | | steps = 52, shift = 0.742, angle = 0.262 degrees |
18699 | | |
18700 | | Position of cryosparc_P126_J4025_004_volume_map_half_B.mrc (#16) relative to |
18701 | | W20_J3623_consensus_structure.mrc (#1) coordinates: |
18702 | | Matrix rotation and translation |
18703 | | 0.99999486 0.00315412 0.00057190 -0.38968890 |
18704 | | -0.00315224 0.99998971 -0.00326115 0.40412911 |
18705 | | -0.00058218 0.00325933 0.99999452 0.02235804 |
18706 | | Axis 0.71306302 0.12620638 -0.68964707 |
18707 | | Axis point 0.00000000 44.63041208 133.15812471 |
18708 | | Rotation angle (degrees) 0.26196640 |
18709 | | Shift along axis -0.24228822 |
18710 | | |
18711 | | |
18712 | | > volume resample #15 #16 #14 onGrid #1 |
18713 | | |
18714 | | Opened cryosparc_P126_J4025_004_volume_map_half_A.mrc resampled as #17, grid |
18715 | | size 240,240,240, pixel 1.19, shown at step 1, values float32 |
18716 | | Opened cryosparc_P126_J4025_004_volume_map_half_B.mrc resampled as #41, grid |
18717 | | size 240,240,240, pixel 1.19, shown at step 1, values float32 |
18718 | | Opened W20_J4025_004_volume_map_bprime_local.mrc resampled as #90, grid size |
18719 | | 240,240,240, pixel 1.19, shown at step 1, values float32 |
18720 | | |
18721 | | > save W20_J4024_halfA_consensus_resampled.mrc #17 |
18722 | | |
18723 | | > hide #!13.2 models |
18724 | | |
18725 | | > hide #!13.1 models |
18726 | | |
18727 | | > show #!13.1 models |
18728 | | |
18729 | | > hide #!13.1 models |
18730 | | |
18731 | | > hide #!13 models |
18732 | | |
18733 | | > hide #!41 models |
18734 | | |
18735 | | > hide #!90 models |
18736 | | |
18737 | | > show #!13.1 models |
18738 | | |
18739 | | > hide #!13.1 models |
18740 | | |
18741 | | > show #!13.1 models |
18742 | | |
18743 | | > hide #!13.1 models |
18744 | | |
18745 | | > show #!13.1 models |
18746 | | |
18747 | | > hide #!13.1 models |
18748 | | |
18749 | | > show #!13.2 models |
18750 | | |
18751 | | > show #!13.1 models |
18752 | | |
18753 | | > hide #!13.1 models |
18754 | | |
18755 | | > hide #!13.2 models |
18756 | | |
18757 | | > save W20_J4024_halfA_consensus_resampled.mrc #13.1 |
18758 | | |
18759 | | > save W20_J4025_halfA_consensus_resampled.mrc #17 |
18760 | | |
18761 | | > rename #17 W20_J4025_halfA_consensus_resampled.mrc |
18762 | | |
18763 | | > show #!90 models |
18764 | | |
18765 | | > show #!41 models |
18766 | | |
18767 | | > save W20_J4025_halfB_consensus_resampled.mrc #41 |
18768 | | |
18769 | | > save W20_J4025_consensus_resampled.mrc #90 |
18770 | | |
18771 | | > hide #!13 models |
18772 | | |
18773 | | > close #14-16 |
18774 | | |
18775 | | > open "/Users/becca/Desktop/Postdoc/COPI notebooks and |
18776 | | > resources/Processing/W20_MM_cyto_COPI/W20_J4025_consensus_resampled.mrc" |
18777 | | |
18778 | | Opened W20_J4025_consensus_resampled.mrc as #14, grid size 240,240,240, pixel |
18779 | | 1.19, shown at level 0.26, step 1, values float32 |
18780 | | |
18781 | | > hide #!17 models |
18782 | | |
18783 | | > open "/Users/becca/Desktop/Postdoc/COPI notebooks and |
18784 | | > resources/Processing/W20_MM_cyto_COPI/W20_J4025_halfA_consensus_resampled.mrc" |
18785 | | > "/Users/becca/Desktop/Postdoc/COPI notebooks and |
18786 | | > resources/Processing/W20_MM_cyto_COPI/W20_J4025_halfB_consensus_resampled.mrc" |
18787 | | |
18788 | | Opened W20_J4025_halfA_consensus_resampled.mrc as #15.1, grid size |
18789 | | 240,240,240, pixel 1.19, shown at level 0.26, step 1, values float32 |
18790 | | Opened W20_J4025_halfB_consensus_resampled.mrc as #15.2, grid size |
18791 | | 240,240,240, pixel 1.19, shown at level 0.26, step 1, values float32 |
18792 | | |
18793 | | > save W20_J4024_halfA_consensus_resampled.mrc #13.1 |
18794 | | |
18795 | | > rename #15 J4025_half_maps |
18796 | | |
18797 | | > hide #!15 models |
18798 | | |
18799 | | > hide #!14 models |
18800 | | |
18801 | | > close #17 |
18802 | | |
18803 | | > close #18 |
18804 | | |
18805 | | > close #19 |
18806 | | |
18807 | | > close #20 |
18808 | | |
18809 | | > close #21 |
18810 | | |
18811 | | > close #22 |
18812 | | |
18813 | | > close #23 |
18814 | | |
18815 | | > hide #!1 models |
18816 | | |
18817 | | > show #!1 models |
18818 | | |
18819 | | > hide #!1 models |
18820 | | |
18821 | | > show #!1 models |
18822 | | |
18823 | | > hide #!1 models |
18824 | | |
18825 | | > show #!2 models |
18826 | | |
18827 | | > hide #!2 models |
18828 | | |
18829 | | > show #!2 models |
18830 | | |
18831 | | > hide #!2 models |
18832 | | |
18833 | | > show #!3 models |
18834 | | |
18835 | | > hide #!3 models |
18836 | | |
18837 | | > open "/Users/becca/Desktop/Postdoc/COPI notebooks and |
18838 | | > resources/Processing/W20_MM_cyto_COPI/cryosparc_P126_J4026_004_volume_map_half_A.mrc" |
18839 | | |
18840 | | Opened cryosparc_P126_J4026_004_volume_map_half_A.mrc as #16, grid size |
18841 | | 240,240,240, pixel 1.19, shown at level 0.266, step 1, values float32 |
18842 | | |
18843 | | > open "/Users/becca/Desktop/Postdoc/COPI notebooks and |
18844 | | > resources/Processing/W20_MM_cyto_COPI/cryosparc_P126_J4026_004_volume_map_half_B.mrc" |
18845 | | |
18846 | | Opened cryosparc_P126_J4026_004_volume_map_half_B.mrc as #17, grid size |
18847 | | 240,240,240, pixel 1.19, shown at level 0.265, step 1, values float32 |
18848 | | |
18849 | | > open "/Users/becca/Desktop/Postdoc/COPI notebooks and |
18850 | | > resources/Processing/W20_MM_cyto_COPI/W20_J4026_004_volume_map_gammazeta_local.mrc" |
18851 | | |
18852 | | Opened W20_J4026_004_volume_map_gammazeta_local.mrc as #18, grid size |
18853 | | 240,240,240, pixel 1.19, shown at level 0.26, step 1, values float32 |
18854 | | |
18855 | | > hide #!17 models |
18856 | | |
18857 | | > show #!17 models |
18858 | | |
18859 | | > hide #!17 models |
18860 | | |
18861 | | > show #!17 models |
18862 | | |
18863 | | > hide #!18 models |
18864 | | |
18865 | | > hide #!41 models |
18866 | | |
18867 | | > hide #!90 models |
18868 | | |
18869 | | > hide #!17 models |
18870 | | |
18871 | | > show #!17 models |
18872 | | |
18873 | | > hide #!17 models |
18874 | | |
18875 | | > hide #!16 models |
18876 | | |
18877 | | > show #!18 models |
18878 | | |
18879 | | > hide #!18 models |
18880 | | |
18881 | | > show #!18 models |
18882 | | |
18883 | | > show #!17 models |
18884 | | |
18885 | | > hide #!17 models |
18886 | | |
18887 | | > show #!16 models |
18888 | | |
18889 | | > hide #!16 models |
18890 | | |
18891 | | > show #!16 models |
18892 | | |
18893 | | > show #!17 models |
18894 | | |
18895 | | > hide #!16 models |
18896 | | |
18897 | | > show #!16 models |
18898 | | |
18899 | | > hide #!17 models |
18900 | | |
18901 | | > show #!17 models |
18902 | | |
18903 | | > fitmap #16 inMap #1 |
18904 | | |
18905 | | Fit map cryosparc_P126_J4026_004_volume_map_half_A.mrc in map |
18906 | | W20_J3623_consensus_structure.mrc using 138044 points |
18907 | | correlation = 0.9061, correlation about mean = 0.2969, overlap = 1.877e+04 |
18908 | | steps = 52, shift = 0.633, angle = 0.336 degrees |
18909 | | |
18910 | | Position of cryosparc_P126_J4026_004_volume_map_half_A.mrc (#16) relative to |
18911 | | W20_J3623_consensus_structure.mrc (#1) coordinates: |
18912 | | Matrix rotation and translation |
18913 | | 0.99999262 0.00086300 -0.00374493 -0.00440355 |
18914 | | -0.00084638 0.99998980 0.00443601 -0.04656357 |
18915 | | 0.00374872 -0.00443280 0.99998315 0.35139305 |
18916 | | Axis -0.75569566 -0.63852116 -0.14565300 |
18917 | | Axis point 0.00000000 78.62510597 13.28594619 |
18918 | | Rotation angle (degrees) 0.33621225 |
18919 | | Shift along axis -0.01812188 |
18920 | | |
18921 | | |
18922 | | > fitmap #17 inMap #1 |
18923 | | |
18924 | | Fit map cryosparc_P126_J4026_004_volume_map_half_B.mrc in map |
18925 | | W20_J3623_consensus_structure.mrc using 138078 points |
18926 | | correlation = 0.906, correlation about mean = 0.2947, overlap = 1.874e+04 |
18927 | | steps = 56, shift = 0.632, angle = 0.326 degrees |
18928 | | |
18929 | | Position of cryosparc_P126_J4026_004_volume_map_half_B.mrc (#17) relative to |
18930 | | W20_J3623_consensus_structure.mrc (#1) coordinates: |
18931 | | Matrix rotation and translation |
18932 | | 0.99999516 0.00097527 -0.00295606 -0.10060790 |
18933 | | -0.00096120 0.99998823 0.00475526 -0.05419298 |
18934 | | 0.00296066 -0.00475240 0.99998432 0.51671634 |
18935 | | Axis -0.83660673 -0.52062903 -0.17039538 |
18936 | | Axis point 0.00000000 109.57038951 9.00298594 |
18937 | | Rotation angle (degrees) 0.32557225 |
18938 | | Shift along axis 0.02433761 |
18939 | | |
18940 | | |
18941 | | > fitmap #18 inMap #1 |
18942 | | |
18943 | | Fit map W20_J4026_004_volume_map_gammazeta_local.mrc in map |
18944 | | W20_J3623_consensus_structure.mrc using 138172 points |
18945 | | correlation = 0.917, correlation about mean = 0.2905, overlap = 1.854e+04 |
18946 | | steps = 52, shift = 0.635, angle = 0.343 degrees |
18947 | | |
18948 | | Position of W20_J4026_004_volume_map_gammazeta_local.mrc (#18) relative to |
18949 | | W20_J3623_consensus_structure.mrc (#1) coordinates: |
18950 | | Matrix rotation and translation |
18951 | | 0.99999273 0.00094755 -0.00369338 -0.01379933 |
18952 | | -0.00093047 0.99998888 0.00462325 -0.05119869 |
18953 | | 0.00369772 -0.00461978 0.99998249 0.38867466 |
18954 | | Axis -0.77135465 -0.61680564 -0.15672526 |
18955 | | Axis point 0.00000000 83.44645675 13.76857176 |
18956 | | Rotation angle (degrees) 0.34328547 |
18957 | | Shift along axis -0.01869132 |
18958 | | |
18959 | | |
18960 | | > volume resample #17 #16 #18 onGrid #1 |
18961 | | |
18962 | | Opened cryosparc_P126_J4026_004_volume_map_half_B.mrc resampled as #19, grid |
18963 | | size 240,240,240, pixel 1.19, shown at step 1, values float32 |
18964 | | Opened cryosparc_P126_J4026_004_volume_map_half_A.mrc resampled as #20, grid |
18965 | | size 240,240,240, pixel 1.19, shown at step 1, values float32 |
18966 | | Opened W20_J4026_004_volume_map_gammazeta_local.mrc resampled as #21, grid |
18967 | | size 240,240,240, pixel 1.19, shown at step 1, values float32 |
18968 | | |
18969 | | > save W20_J4026_halfA_consensus_resampled.mrc #20 |
18970 | | |
18971 | | > save W20_J4026_halfB_consensus_resampled.mrc #19 |
18972 | | |
18973 | | > save W20_J4026_consensus_resampled.mrc #21 |
18974 | | |
18975 | | > close #16-18 |
18976 | | |
18977 | | > open "/Users/becca/Desktop/Postdoc/COPI notebooks and |
18978 | | > resources/Processing/W20_MM_cyto_COPI/W20_J4026_consensus_resampled.mrc" |
18979 | | |
18980 | | Opened W20_J4026_consensus_resampled.mrc as #16, grid size 240,240,240, pixel |
18981 | | 1.19, shown at level 0.26, step 1, values float32 |
18982 | | |
18983 | | > hide #!21 models |
18984 | | |
18985 | | > hide #!20 models |
18986 | | |
18987 | | > hide #!19 models |
18988 | | |
18989 | | > open "/Users/becca/Desktop/Postdoc/COPI notebooks and |
18990 | | > resources/Processing/W20_MM_cyto_COPI/W20_J4026_halfA_consensus_resampled.mrc" |
18991 | | > "/Users/becca/Desktop/Postdoc/COPI notebooks and |
18992 | | > resources/Processing/W20_MM_cyto_COPI/W20_J4026_halfB_consensus_resampled.mrc" |
18993 | | |
18994 | | Opened W20_J4026_halfA_consensus_resampled.mrc as #17.1, grid size |
18995 | | 240,240,240, pixel 1.19, shown at level 0.261, step 1, values float32 |
18996 | | Opened W20_J4026_halfB_consensus_resampled.mrc as #17.2, grid size |
18997 | | 240,240,240, pixel 1.19, shown at level 0.26, step 1, values float32 |
18998 | | |
18999 | | > rename #17 J4026_half_maps |
19000 | | |
19001 | | > close #19-21 |
19002 | | |
19003 | | > open "/Users/becca/Desktop/Postdoc/COPI notebooks and |
19004 | | > resources/Processing/W20_MM_cyto_COPI/cryosparc_P126_J4027_005_volume_map_half_A.mrc" |
19005 | | |
19006 | | Opened cryosparc_P126_J4027_005_volume_map_half_A.mrc as #18, grid size |
19007 | | 240,240,240, pixel 1.19, shown at level 0.26, step 1, values float32 |
19008 | | |
19009 | | > open "/Users/becca/Desktop/Postdoc/COPI notebooks and |
19010 | | > resources/Processing/W20_MM_cyto_COPI/cryosparc_P126_J4027_005_volume_map_half_B.mrc" |
19011 | | |
19012 | | Opened cryosparc_P126_J4027_005_volume_map_half_B.mrc as #19, grid size |
19013 | | 240,240,240, pixel 1.19, shown at level 0.259, step 1, values float32 |
19014 | | |
19015 | | > open "/Users/becca/Desktop/Postdoc/COPI notebooks and |
19016 | | > resources/Processing/W20_MM_cyto_COPI/W20_J4027_005_volume_map_gammazeta_local.mrc" |
19017 | | |
19018 | | Opened W20_J4027_005_volume_map_gammazeta_local.mrc as #20, grid size |
19019 | | 240,240,240, pixel 1.19, shown at level 0.253, step 1, values float32 |
19020 | | |
19021 | | > hide #!17 models |
19022 | | |
19023 | | > hide #!16 models |
19024 | | |
19025 | | > fitmap #18 inMap #1 |
19026 | | |
19027 | | Fit map cryosparc_P126_J4027_005_volume_map_half_A.mrc in map |
19028 | | W20_J3623_consensus_structure.mrc using 138063 points |
19029 | | correlation = 0.9242, correlation about mean = 0.4068, overlap = 1.895e+04 |
19030 | | steps = 64, shift = 0.865, angle = 0.418 degrees |
19031 | | |
19032 | | Position of cryosparc_P126_J4027_005_volume_map_half_A.mrc (#18) relative to |
19033 | | W20_J3623_consensus_structure.mrc (#1) coordinates: |
19034 | | Matrix rotation and translation |
19035 | | 0.99997669 -0.00229559 0.00643030 -1.04477946 |
19036 | | 0.00227893 0.99999403 0.00259782 -0.33276084 |
19037 | | -0.00643622 -0.00258311 0.99997595 1.91301880 |
19038 | | Axis -0.35472898 0.88094699 0.31320880 |
19039 | | Axis point 263.82795379 0.00000000 126.10679381 |
19040 | | Rotation angle (degrees) 0.41841556 |
19041 | | Shift along axis 0.67664322 |
19042 | | |
19043 | | |
19044 | | > fitmap #19 inMap #1 |
19045 | | |
19046 | | Fit map cryosparc_P126_J4027_005_volume_map_half_B.mrc in map |
19047 | | W20_J3623_consensus_structure.mrc using 138195 points |
19048 | | correlation = 0.9253, correlation about mean = 0.4118, overlap = 1.9e+04 |
19049 | | steps = 68, shift = 0.852, angle = 0.431 degrees |
19050 | | |
19051 | | Position of cryosparc_P126_J4027_005_volume_map_half_B.mrc (#19) relative to |
19052 | | W20_J3623_consensus_structure.mrc (#1) coordinates: |
19053 | | Matrix rotation and translation |
19054 | | 0.99997498 -0.00277336 0.00650725 -0.98066980 |
19055 | | 0.00275654 0.99999284 0.00259285 -0.39125472 |
19056 | | -0.00651439 -0.00257484 0.99997547 1.90493875 |
19057 | | Axis -0.34310644 0.86456626 0.36715549 |
19058 | | Axis point 252.66913251 0.00000000 114.89251767 |
19059 | | Rotation angle (degrees) 0.43148350 |
19060 | | Shift along axis 0.69761723 |
19061 | | |
19062 | | |
19063 | | > fitmap #20 inMap #1 |
19064 | | |
19065 | | Fit map W20_J4027_005_volume_map_gammazeta_local.mrc in map |
19066 | | W20_J3623_consensus_structure.mrc using 138160 points |
19067 | | correlation = 0.9373, correlation about mean = 0.4299, overlap = 1.864e+04 |
19068 | | steps = 64, shift = 0.871, angle = 0.411 degrees |
19069 | | |
19070 | | Position of W20_J4027_005_volume_map_gammazeta_local.mrc (#20) relative to |
19071 | | W20_J3623_consensus_structure.mrc (#1) coordinates: |
19072 | | Matrix rotation and translation |
19073 | | 0.99997680 -0.00234496 0.00639584 -1.04246365 |
19074 | | 0.00233059 0.99999474 0.00225441 -0.28336984 |
19075 | | -0.00640109 -0.00223945 0.99997701 1.85486702 |
19076 | | Axis -0.31324184 0.89200052 0.32590584 |
19077 | | Axis point 254.76967129 0.00000000 130.69543046 |
19078 | | Rotation angle (degrees) 0.41099531 |
19079 | | Shift along axis 0.67828918 |
19080 | | |
19081 | | |
19082 | | > volume resample #19 #20 #18 onGrid #1 |
19083 | | |
19084 | | Opened cryosparc_P126_J4027_005_volume_map_half_B.mrc resampled as #21, grid |
19085 | | size 240,240,240, pixel 1.19, shown at step 1, values float32 |
19086 | | Opened W20_J4027_005_volume_map_gammazeta_local.mrc resampled as #22, grid |
19087 | | size 240,240,240, pixel 1.19, shown at step 1, values float32 |
19088 | | Opened cryosparc_P126_J4027_005_volume_map_half_A.mrc resampled as #23, grid |
19089 | | size 240,240,240, pixel 1.19, shown at step 1, values float32 |
19090 | | |
19091 | | > save W20_J4027_halfB_consensus_resampled.mrc #21 |
19092 | | |
19093 | | > save W20_J4027_halfA_consensus_resampled.mrc #23 |
19094 | | |
19095 | | > save W20_J4027_consensus_resampled.mrc #22 |
19096 | | |
19097 | | > close #18-20 |
19098 | | |
19099 | | > open "/Users/becca/Desktop/Postdoc/COPI notebooks and |
19100 | | > resources/Processing/W20_MM_cyto_COPI/W20_J4027_consensus_resampled.mrc" |
19101 | | |
19102 | | Opened W20_J4027_consensus_resampled.mrc as #18, grid size 240,240,240, pixel |
19103 | | 1.19, shown at level 0.253, step 1, values float32 |
19104 | | |
19105 | | > open "/Users/becca/Desktop/Postdoc/COPI notebooks and |
19106 | | > resources/Processing/W20_MM_cyto_COPI/W20_J4027_halfA_consensus_resampled.mrc" |
19107 | | > "/Users/becca/Desktop/Postdoc/COPI notebooks and |
19108 | | > resources/Processing/W20_MM_cyto_COPI/W20_J4027_halfB_consensus_resampled.mrc" |
19109 | | |
19110 | | Opened W20_J4027_halfA_consensus_resampled.mrc as #19.1, grid size |
19111 | | 240,240,240, pixel 1.19, shown at level 0.254, step 1, values float32 |
19112 | | Opened W20_J4027_halfB_consensus_resampled.mrc as #19.2, grid size |
19113 | | 240,240,240, pixel 1.19, shown at level 0.253, step 1, values float32 |
19114 | | |
19115 | | > rename #19 J4017_half_maps |
19116 | | |
19117 | | > rename #19 J4027_half_maps |
19118 | | |
19119 | | > close #21-23 |
19120 | | |
19121 | | > open "/Users/becca/Desktop/Postdoc/COPI notebooks and |
19122 | | > resources/Processing/W20_MM_cyto_COPI/cryosparc_P126_J4028_004_volume_map_half_A.mrc" |
19123 | | |
19124 | | Opened cryosparc_P126_J4028_004_volume_map_half_A.mrc as #20, grid size |
19125 | | 240,240,240, pixel 1.19, shown at level 0.261, step 1, values float32 |
19126 | | |
19127 | | > open "/Users/becca/Desktop/Postdoc/COPI notebooks and |
19128 | | > resources/Processing/W20_MM_cyto_COPI/cryosparc_P126_J4028_004_volume_map_half_B.mrc" |
19129 | | |
19130 | | Opened cryosparc_P126_J4028_004_volume_map_half_B.mrc as #21, grid size |
19131 | | 240,240,240, pixel 1.19, shown at level 0.261, step 1, values float32 |
19132 | | |
19133 | | > open "/Users/becca/Desktop/Postdoc/COPI notebooks and |
19134 | | > resources/Processing/W20_MM_cyto_COPI/W20_J4028_004_volume_map_gammazeta_local.mrc" |
19135 | | |
19136 | | Opened W20_J4028_004_volume_map_gammazeta_local.mrc as #22, grid size |
19137 | | 240,240,240, pixel 1.19, shown at level 0.255, step 1, values float32 |
19138 | | |
19139 | | > hide #!19 models |
19140 | | |
19141 | | > hide #!18 models |
19142 | | |
19143 | | > fitmap #20 inMap #1 |
19144 | | |
19145 | | Fit map cryosparc_P126_J4028_004_volume_map_half_A.mrc in map |
19146 | | W20_J3623_consensus_structure.mrc using 138211 points |
19147 | | correlation = 0.9192, correlation about mean = 0.3816, overlap = 1.899e+04 |
19148 | | steps = 52, shift = 0.522, angle = 0.296 degrees |
19149 | | |
19150 | | Position of cryosparc_P126_J4028_004_volume_map_half_A.mrc (#20) relative to |
19151 | | W20_J3623_consensus_structure.mrc (#1) coordinates: |
19152 | | Matrix rotation and translation |
19153 | | 0.99998706 0.00198963 -0.00468116 0.25998221 |
19154 | | -0.00198544 0.99999762 0.00090051 0.55733772 |
19155 | | 0.00468294 -0.00089120 0.99998864 -0.22070790 |
19156 | | Axis -0.17345663 -0.90654298 -0.38482804 |
19157 | | Axis point 85.46846238 0.00000000 38.05634596 |
19158 | | Rotation angle (degrees) 0.29591860 |
19159 | | Shift along axis -0.46541164 |
19160 | | |
19161 | | |
19162 | | > fitmap #21 inMap #1 |
19163 | | |
19164 | | Fit map cryosparc_P126_J4028_004_volume_map_half_B.mrc in map |
19165 | | W20_J3623_consensus_structure.mrc using 138125 points |
19166 | | correlation = 0.9186, correlation about mean = 0.3805, overlap = 1.898e+04 |
19167 | | steps = 48, shift = 0.516, angle = 0.21 degrees |
19168 | | |
19169 | | Position of cryosparc_P126_J4028_004_volume_map_half_B.mrc (#21) relative to |
19170 | | W20_J3623_consensus_structure.mrc (#1) coordinates: |
19171 | | Matrix rotation and translation |
19172 | | 0.99999365 0.00064252 -0.00350470 0.30153938 |
19173 | | -0.00063949 0.99999942 0.00086460 0.34856397 |
19174 | | 0.00350526 -0.00086235 0.99999348 -0.04103359 |
19175 | | Axis -0.23552090 -0.95601319 -0.17483906 |
19176 | | Axis point 31.62231369 0.00000000 59.29711678 |
19177 | | Rotation angle (degrees) 0.21006100 |
19178 | | Shift along axis -0.39707630 |
19179 | | |
19180 | | |
19181 | | > fitmap #22 inMap #1 |
19182 | | |
19183 | | Fit map W20_J4028_004_volume_map_gammazeta_local.mrc in map |
19184 | | W20_J3623_consensus_structure.mrc using 138218 points |
19185 | | correlation = 0.9302, correlation about mean = 0.3927, overlap = 1.873e+04 |
19186 | | steps = 52, shift = 0.535, angle = 0.265 degrees |
19187 | | |
19188 | | Position of W20_J4028_004_volume_map_gammazeta_local.mrc (#22) relative to |
19189 | | W20_J3623_consensus_structure.mrc (#1) coordinates: |
19190 | | Matrix rotation and translation |
19191 | | 0.99998992 0.00112578 -0.00434648 0.33637900 |
19192 | | -0.00112111 0.99999879 0.00107657 0.40418343 |
19193 | | 0.00434769 -0.00107168 0.99998997 -0.12603288 |
19194 | | Axis -0.23266548 -0.94161994 -0.24334886 |
19195 | | Axis point 53.07995958 0.00000000 54.34764883 |
19196 | | Rotation angle (degrees) 0.26451275 |
19197 | | Shift along axis -0.42818100 |
19198 | | |
19199 | | |
19200 | | > volume resample #21 #20 #22 onGrid #1 |
19201 | | |
19202 | | Opened cryosparc_P126_J4028_004_volume_map_half_B.mrc resampled as #23, grid |
19203 | | size 240,240,240, pixel 1.19, shown at step 1, values float32 |
19204 | | Opened cryosparc_P126_J4028_004_volume_map_half_A.mrc resampled as #91, grid |
19205 | | size 240,240,240, pixel 1.19, shown at step 1, values float32 |
19206 | | Opened W20_J4028_004_volume_map_gammazeta_local.mrc resampled as #92, grid |
19207 | | size 240,240,240, pixel 1.19, shown at step 1, values float32 |
19208 | | |
19209 | | > save W20_J4028_halfA_consensus_resampled.mrc #91 |
19210 | | |
19211 | | > save W20_J4028_halfB_consensus_resampled.mrc #23 |
19212 | | |
19213 | | > save W20_J4028_consensus_resampled.mrc #92 |
19214 | | |
19215 | | > close #20-22 |
19216 | | |
19217 | | > open "/Users/becca/Desktop/Postdoc/COPI notebooks and |
19218 | | > resources/Processing/W20_MM_cyto_COPI/W20_J4028_consensus_resampled.mrc" |
19219 | | |
19220 | | Opened W20_J4028_consensus_resampled.mrc as #20, grid size 240,240,240, pixel |
19221 | | 1.19, shown at level 0.255, step 1, values float32 |
19222 | | |
19223 | | > open "/Users/becca/Desktop/Postdoc/COPI notebooks and |
19224 | | > resources/Processing/W20_MM_cyto_COPI/W20_J4028_halfA_consensus_resampled.mrc" |
19225 | | > "/Users/becca/Desktop/Postdoc/COPI notebooks and |
19226 | | > resources/Processing/W20_MM_cyto_COPI/W20_J4028_halfB_consensus_resampled.mrc" |
19227 | | |
19228 | | Opened W20_J4028_halfA_consensus_resampled.mrc as #21.1, grid size |
19229 | | 240,240,240, pixel 1.19, shown at level 0.256, step 1, values float32 |
19230 | | Opened W20_J4028_halfB_consensus_resampled.mrc as #21.2, grid size |
19231 | | 240,240,240, pixel 1.19, shown at level 0.256, step 1, values float32 |
19232 | | |
19233 | | > rename #21 J4028_half_maps |
19234 | | |
19235 | | > combine #24 |
19236 | | |
19237 | | [Repeated 1 time(s)] |
19238 | | |
19239 | | > close #22-24 |
19240 | | |
19241 | | > close #25 |
19242 | | |
19243 | | > close #26 |
19244 | | |
19245 | | > close #27 |
19246 | | |
19247 | | > hide #!93 models |
19248 | | |
19249 | | > hide #!92 models |
19250 | | |
19251 | | > open "/Users/becca/Desktop/Postdoc/COPI notebooks and |
19252 | | > resources/Processing/W20_MM_cyto_COPI/cryosparc_P126_J3988_003_volume_map_half_A.mrc" |
19253 | | |
19254 | | Opened cryosparc_P126_J3988_003_volume_map_half_A.mrc as #22, grid size |
19255 | | 240,240,240, pixel 1.2, shown at level 0.291, step 1, values float32 |
19256 | | |
19257 | | > open "/Users/becca/Desktop/Postdoc/COPI notebooks and |
19258 | | > resources/Processing/W20_MM_cyto_COPI/cryosparc_P126_J3988_003_volume_map_half_B.mrc" |
19259 | | |
19260 | | Opened cryosparc_P126_J3988_003_volume_map_half_B.mrc as #23, grid size |
19261 | | 240,240,240, pixel 1.2, shown at level 0.291, step 1, values float32 |
19262 | | |
19263 | | > select add #22 |
19264 | | |
19265 | | 2 models selected |
19266 | | |
19267 | | > select add #23 |
19268 | | |
19269 | | 4 models selected |
19270 | | |
19271 | | > hide #!21 models |
19272 | | |
19273 | | > hide #!20 models |
19274 | | |
19275 | | > ui mousemode right "rotate selected models" |
19276 | | |
19277 | | > view matrix models |
19278 | | > #22,-0.85576,-0.11016,-0.5055,355.25,-0.33846,0.85818,0.38596,14.029,0.3913,0.50138,-0.77169,124.47,#23,-0.85576,-0.11016,-0.5055,355.25,-0.33846,0.85818,0.38596,14.029,0.3913,0.50138,-0.77169,124.47 |
19279 | | |
19280 | | > view matrix models |
19281 | | > #22,-0.53192,-0.84561,0.044824,336.27,-0.68771,0.46227,0.55979,96.534,-0.49408,0.26694,-0.82742,293.75,#23,-0.53192,-0.84561,0.044824,336.27,-0.68771,0.46227,0.55979,96.534,-0.49408,0.26694,-0.82742,293.75 |
19282 | | |
19283 | | > ui mousemode right "translate selected models" |
19284 | | |
19285 | | > view matrix models |
19286 | | > #22,-0.53192,-0.84561,0.044824,335.35,-0.68771,0.46227,0.55979,94.798,-0.49408,0.26694,-0.82742,289.5,#23,-0.53192,-0.84561,0.044824,335.35,-0.68771,0.46227,0.55979,94.798,-0.49408,0.26694,-0.82742,289.5 |
19287 | | |
19288 | | > view matrix models |
19289 | | > #22,-0.53192,-0.84561,0.044824,337.81,-0.68771,0.46227,0.55979,89.918,-0.49408,0.26694,-0.82742,289.61,#23,-0.53192,-0.84561,0.044824,337.81,-0.68771,0.46227,0.55979,89.918,-0.49408,0.26694,-0.82742,289.61 |
19290 | | |
19291 | | > hide #!91 models |
19292 | | |
19293 | | > open "/Users/becca/Desktop/Postdoc/COPI notebooks and |
19294 | | > resources/Processing/W20_MM_cyto_COPI/W20_J3988_004_volume_map_csparc_refine_of_relion_job126_gammazeta_mask.mrc" |
19295 | | |
19296 | | Opened |
19297 | | W20_J3988_004_volume_map_csparc_refine_of_relion_job126_gammazeta_mask.mrc as |
19298 | | #24, grid size 240,240,240, pixel 1.2, shown at level 0.282, step 1, values |
19299 | | float32 |
19300 | | |
19301 | | > select add #24 |
19302 | | |
19303 | | 6 models selected |
19304 | | |
19305 | | > select subtract #23 |
19306 | | |
19307 | | 4 models selected |
19308 | | |
19309 | | > select subtract #22 |
19310 | | |
19311 | | 2 models selected |
19312 | | |
19313 | | > view matrix models #24,1,0,0,4.4068,0,1,0,-1.5627,0,0,1,7.0623 |
19314 | | |
19315 | | > show #!1 models |
19316 | | |
19317 | | > ui mousemode right "rotate selected models" |
19318 | | |
19319 | | > view matrix models |
19320 | | > #24,-0.59721,0.29169,0.74717,82.383,0.43729,-0.66251,0.60816,89.121,0.6724,0.68993,0.2681,-83.296 |
19321 | | |
19322 | | > view matrix models |
19323 | | > #24,0.58998,-0.6462,-0.4841,224.91,-0.8066,-0.49879,-0.31719,374.29,-0.036497,0.57761,-0.8155,188.31 |
19324 | | |
19325 | | > view matrix models |
19326 | | > #24,-0.46477,-0.87473,-0.13728,358.04,-0.68335,0.25576,0.68383,105.04,-0.56305,0.41163,-0.71661,272.86 |
19327 | | |
19328 | | > fitmap #22 inMap #1 |
19329 | | |
19330 | | Fit map cryosparc_P126_J3988_003_volume_map_half_A.mrc in map |
19331 | | W20_J3623_consensus_structure.mrc using 138175 points |
19332 | | correlation = 0.9519, correlation about mean = 0.5985, overlap = 2.143e+04 |
19333 | | steps = 320, shift = 8.89, angle = 19.2 degrees |
19334 | | |
19335 | | Position of cryosparc_P126_J3988_003_volume_map_half_A.mrc (#22) relative to |
19336 | | W20_J3623_consensus_structure.mrc (#1) coordinates: |
19337 | | Matrix rotation and translation |
19338 | | -0.33642694 -0.92057260 -0.19840110 353.49844057 |
19339 | | -0.77937135 0.15392648 0.60736063 147.09880294 |
19340 | | -0.52858037 0.35896062 -0.76925292 278.05341217 |
19341 | | Axis -0.56888343 0.75617354 0.32337782 |
19342 | | Axis point 252.39557005 0.00000000 81.74420969 |
19343 | | Rotation angle (degrees) 167.38948043 |
19344 | | Shift along axis 0.04912198 |
19345 | | |
19346 | | |
19347 | | > fitmap #23 inMap #1 |
19348 | | |
19349 | | Fit map cryosparc_P126_J3988_003_volume_map_half_B.mrc in map |
19350 | | W20_J3623_consensus_structure.mrc using 138208 points |
19351 | | correlation = 0.9526, correlation about mean = 0.6, overlap = 2.142e+04 |
19352 | | steps = 324, shift = 8.99, angle = 19.2 degrees |
19353 | | |
19354 | | Position of cryosparc_P126_J3988_003_volume_map_half_B.mrc (#23) relative to |
19355 | | W20_J3623_consensus_structure.mrc (#1) coordinates: |
19356 | | Matrix rotation and translation |
19357 | | -0.33657596 -0.92064163 -0.19782722 353.45179614 |
19358 | | -0.77865335 0.15396136 0.60827203 146.98371640 |
19359 | | -0.52954280 0.35876857 -0.76868038 278.15909277 |
19360 | | Axis -0.56874816 0.75615237 0.32366516 |
19361 | | Axis point 252.42080897 0.00000000 81.66752530 |
19362 | | Rotation angle (degrees) 167.32947007 |
19363 | | Shift along axis 0.14743379 |
19364 | | |
19365 | | |
19366 | | > fitmap #24 inMap #1 |
19367 | | |
19368 | | Fit map |
19369 | | W20_J3988_004_volume_map_csparc_refine_of_relion_job126_gammazeta_mask.mrc in |
19370 | | map W20_J3623_consensus_structure.mrc using 138204 points |
19371 | | correlation = 0.9643, correlation about mean = 0.6577, overlap = 2.107e+04 |
19372 | | steps = 104, shift = 5.31, angle = 9.46 degrees |
19373 | | |
19374 | | Position of |
19375 | | W20_J3988_004_volume_map_csparc_refine_of_relion_job126_gammazeta_mask.mrc |
19376 | | (#24) relative to W20_J3623_consensus_structure.mrc (#1) coordinates: |
19377 | | Matrix rotation and translation |
19378 | | -0.33645113 -0.92061038 -0.19818457 353.46943026 |
19379 | | -0.77924371 0.15400594 0.60750421 147.10114448 |
19380 | | -0.52875310 0.35882956 -0.76919534 278.08170115 |
19381 | | Axis -0.56885571 0.75619205 0.32338333 |
19382 | | Axis point 252.40251661 0.00000000 81.72856043 |
19383 | | Rotation angle (degrees) 167.37468234 |
19384 | | Shift along axis 0.09059859 |
19385 | | |
19386 | | |
19387 | | > select subtract #24 |
19388 | | |
19389 | | Nothing selected |
19390 | | |
19391 | | > volume resample #24 #23 #22 onGrid #1 |
19392 | | |
19393 | | Opened |
19394 | | W20_J3988_004_volume_map_csparc_refine_of_relion_job126_gammazeta_mask.mrc |
19395 | | resampled as #25, grid size 240,240,240, pixel 1.19, shown at step 1, values |
19396 | | float32 |
19397 | | Opened cryosparc_P126_J3988_003_volume_map_half_B.mrc resampled as #26, grid |
19398 | | size 240,240,240, pixel 1.19, shown at step 1, values float32 |
19399 | | Opened cryosparc_P126_J3988_003_volume_map_half_A.mrc resampled as #27, grid |
19400 | | size 240,240,240, pixel 1.19, shown at step 1, values float32 |
19401 | | |
19402 | | > save W20_J3988_halfB_consensus_resampled.mrc #26 |
19403 | | |
19404 | | > save W20_J3988_halfA_consensus_resampled.mrc #27 |
19405 | | |
19406 | | > save W20_J3988_consensus_resampled.mrc #25 |
19407 | | |
19408 | | > close #22-24 |
19409 | | |
19410 | | > hide #!25 models |
19411 | | |
19412 | | > hide #!26 models |
19413 | | |
19414 | | > hide #!27 models |
19415 | | |
19416 | | > open "/Users/becca/Desktop/Postdoc/COPI notebooks and |
19417 | | > resources/Processing/W20_MM_cyto_COPI/W20_J3988_consensus_resampled.mrc" |
19418 | | |
19419 | | Opened W20_J3988_consensus_resampled.mrc as #22, grid size 240,240,240, pixel |
19420 | | 1.19, shown at level 0.286, step 1, values float32 |
19421 | | |
19422 | | > open "/Users/becca/Desktop/Postdoc/COPI notebooks and |
19423 | | > resources/Processing/W20_MM_cyto_COPI/W20_J3988_halfA_consensus_resampled.mrc" |
19424 | | > "/Users/becca/Desktop/Postdoc/COPI notebooks and |
19425 | | > resources/Processing/W20_MM_cyto_COPI/W20_J3988_halfB_consensus_resampled.mrc" |
19426 | | |
19427 | | Opened W20_J3988_halfA_consensus_resampled.mrc as #23.1, grid size |
19428 | | 240,240,240, pixel 1.19, shown at level 0.29, step 1, values float32 |
19429 | | Opened W20_J3988_halfB_consensus_resampled.mrc as #23.2, grid size |
19430 | | 240,240,240, pixel 1.19, shown at level 0.29, step 1, values float32 |
19431 | | |
19432 | | > rename #23 J3988_half_maps |
19433 | | |
19434 | | > close #25-27 |
19435 | | |
19436 | | > show #!28 models |
19437 | | |
19438 | | > hide #!28 models |
19439 | | |
19440 | | > rename #28 COPI_cyto_for_J4027_v1 |
19441 | | |
19442 | | > hide #!23 models |
19443 | | |
19444 | | > hide #!22 models |
19445 | | |
19446 | | > save "/Users/becca/Desktop/Postdoc/COPI notebooks and |
19447 | | > resources/Processing/W20_MM_cyto_COPI/20250707_W20_COPI_cyto_alignments.cxs" |
19448 | | |
19449 | | > open |
19450 | | > /Users/becca/Desktop/Postdoc/Phenix/W20_MM_cyto_COPI/LocalResolution_21/W20_J3623_consensus_structure_full.ccp4 |
19451 | | |
19452 | | Opened W20_J3623_consensus_structure_full.ccp4 as #24, grid size 240,240,240, |
19453 | | pixel 1.19, shown at level 0.257, step 1, values float32 |
19454 | | |
19455 | | > open |
19456 | | > /Users/becca/Desktop/Postdoc/Phenix/W20_MM_cyto_COPI/LocalResolution_21/W20_J3623_consensus_structure_local_resolution.ccp4 |
19457 | | |
19458 | | Opened W20_J3623_consensus_structure_local_resolution.ccp4 as #25, grid size |
19459 | | 240,240,240, pixel 1.19, shown at level 14, step 1, values float32 |
19460 | | |
19461 | | > hide #!25 models |
19462 | | |
19463 | | > show #!25 models |
19464 | | |
19465 | | > hide #!25 models |
19466 | | |
19467 | | > hide #!24 models |
19468 | | |
19469 | | > show #!24 models |
19470 | | |
19471 | | > hide #!1 models |
19472 | | |
19473 | | > color sample #24 map #2 palette |
19474 | | > 2.0,purple:2.3,indigo:2.5,blue:2.8,cyan:3.0,lime:3.2, yellow:3.5, |
19475 | | > red:4.0,salmon:5.0,pink |
19476 | | |
19477 | | Invalid "palette" argument: Missing color name or specifier |
19478 | | |
19479 | | > color sample #24 map #2 |
19480 | | |
19481 | | Map values for surface "surface": minimum -19.22, mean 2.61, maximum 22.06 |
19482 | | |
19483 | | > close #24-25 |
19484 | | |
19485 | | > save "/Users/becca/Desktop/Postdoc/COPI notebooks and |
19486 | | > resources/Processing/W20_MM_cyto_COPI/20250707_W20_COPI_cyto_alignments.cxs" |
19487 | | |
19488 | | > combine #27 |
19489 | | |
19490 | | No structures specified |
19491 | | |
19492 | | > combine #29 |
19493 | | |
19494 | | No structures specified |
19495 | | |
19496 | | > combine #28 |
19497 | | |
19498 | | > rename #24 COPI_cyto_for_J4027_v1 |
19499 | | |
19500 | | > split #24 chains |
19501 | | |
19502 | | Split COPI_cyto_for_J4027_v1 (#24) into 13 models |
19503 | | Chain information for COPI_cyto_for_J4027_v1 A #24.1 |
19504 | | --- |
19505 | | Chain | Description |
19506 | | A | No description available |
19507 | | |
19508 | | Chain information for COPI_cyto_for_J4027_v1 B #24.2 |
19509 | | --- |
19510 | | Chain | Description |
19511 | | B | No description available |
19512 | | |
19513 | | Chain information for COPI_cyto_for_J4027_v1 C #24.3 |
19514 | | --- |
19515 | | Chain | Description |
19516 | | C | No description available |
19517 | | |
19518 | | Chain information for COPI_cyto_for_J4027_v1 D #24.4 |
19519 | | --- |
19520 | | Chain | Description |
19521 | | D | No description available |
19522 | | |
19523 | | Chain information for COPI_cyto_for_J4027_v1 E #24.5 |
19524 | | --- |
19525 | | Chain | Description |
19526 | | E | No description available |
19527 | | |
19528 | | Chain information for COPI_cyto_for_J4027_v1 F #24.6 |
19529 | | --- |
19530 | | Chain | Description |
19531 | | F | No description available |
19532 | | |
19533 | | Chain information for COPI_cyto_for_J4027_v1 G #24.7 |
19534 | | --- |
19535 | | Chain | Description |
19536 | | G | No description available |
19537 | | |
19538 | | Chain information for COPI_cyto_for_J4027_v1 H #24.8 |
19539 | | --- |
19540 | | Chain | Description |
19541 | | H | No description available |
19542 | | |
19543 | | Chain information for COPI_cyto_for_J4027_v1 I #24.9 |
19544 | | --- |
19545 | | Chain | Description |
19546 | | I | No description available |
19547 | | |
19548 | | Chain information for COPI_cyto_for_J4027_v1 J #24.10 |
19549 | | --- |
19550 | | Chain | Description |
19551 | | J | No description available |
19552 | | |
19553 | | Chain information for COPI_cyto_for_J4027_v1 K #24.11 |
19554 | | --- |
19555 | | Chain | Description |
19556 | | K | No description available |
19557 | | |
19558 | | Chain information for COPI_cyto_for_J4027_v1 L #24.12 |
19559 | | --- |
19560 | | Chain | Description |
19561 | | L | No description available |
19562 | | |
19563 | | Chain information for COPI_cyto_for_J4027_v1 M #24.13 |
19564 | | --- |
19565 | | Chain | Description |
19566 | | M | No description available |
19567 | | |
19568 | | |
19569 | | > show #!1 models |
19570 | | |
19571 | | > color #1 darkgrey models |
19572 | | |
19573 | | > color #1 #a9a9a9c0 models |
19574 | | |
19575 | | > rename #24 COPI_cyto_J3623_model |
19576 | | |
19577 | | > hide #!24 models |
19578 | | |
19579 | | > show #!24 models |
19580 | | |
19581 | | > hide #!1 models |
19582 | | |
19583 | | > hide #!24 models |
19584 | | |
19585 | | > combine #28 |
19586 | | |
19587 | | > split #25 chains |
19588 | | |
19589 | | Split copy of COPI_cyto_for_J4027_v1 (#25) into 13 models |
19590 | | Chain information for copy of COPI_cyto_for_J4027_v1 A #25.1 |
19591 | | --- |
19592 | | Chain | Description |
19593 | | A | No description available |
19594 | | |
19595 | | Chain information for copy of COPI_cyto_for_J4027_v1 B #25.2 |
19596 | | --- |
19597 | | Chain | Description |
19598 | | B | No description available |
19599 | | |
19600 | | Chain information for copy of COPI_cyto_for_J4027_v1 C #25.3 |
19601 | | --- |
19602 | | Chain | Description |
19603 | | C | No description available |
19604 | | |
19605 | | Chain information for copy of COPI_cyto_for_J4027_v1 D #25.4 |
19606 | | --- |
19607 | | Chain | Description |
19608 | | D | No description available |
19609 | | |
19610 | | Chain information for copy of COPI_cyto_for_J4027_v1 E #25.5 |
19611 | | --- |
19612 | | Chain | Description |
19613 | | E | No description available |
19614 | | |
19615 | | Chain information for copy of COPI_cyto_for_J4027_v1 F #25.6 |
19616 | | --- |
19617 | | Chain | Description |
19618 | | F | No description available |
19619 | | |
19620 | | Chain information for copy of COPI_cyto_for_J4027_v1 G #25.7 |
19621 | | --- |
19622 | | Chain | Description |
19623 | | G | No description available |
19624 | | |
19625 | | Chain information for copy of COPI_cyto_for_J4027_v1 H #25.8 |
19626 | | --- |
19627 | | Chain | Description |
19628 | | H | No description available |
19629 | | |
19630 | | Chain information for copy of COPI_cyto_for_J4027_v1 I #25.9 |
19631 | | --- |
19632 | | Chain | Description |
19633 | | I | No description available |
19634 | | |
19635 | | Chain information for copy of COPI_cyto_for_J4027_v1 J #25.10 |
19636 | | --- |
19637 | | Chain | Description |
19638 | | J | No description available |
19639 | | |
19640 | | Chain information for copy of COPI_cyto_for_J4027_v1 K #25.11 |
19641 | | --- |
19642 | | Chain | Description |
19643 | | K | No description available |
19644 | | |
19645 | | Chain information for copy of COPI_cyto_for_J4027_v1 L #25.12 |
19646 | | --- |
19647 | | Chain | Description |
19648 | | L | No description available |
19649 | | |
19650 | | Chain information for copy of COPI_cyto_for_J4027_v1 M #25.13 |
19651 | | --- |
19652 | | Chain | Description |
19653 | | M | No description available |
19654 | | |
19655 | | |
19656 | | > show #!4 models |
19657 | | |
19658 | | > rename #25 COPI_cyto_for_J4027_v1 |
19659 | | |
19660 | | > rename #25 COPI_cyto_J3987_model |
19661 | | |
19662 | | > fitmap #25.1 inMap #4 |
19663 | | |
19664 | | Fit molecule copy of COPI_cyto_for_J4027_v1 A (#25.1) to map |
19665 | | W20_J3987_consensus_resampled.mrc (#4) using 2590 atoms |
19666 | | average map value = 0.3221, steps = 104 |
19667 | | shifted from previous position = 0.721 |
19668 | | rotated from previous position = 1.24 degrees |
19669 | | atoms outside contour = 795, contour level = 0.27665 |
19670 | | |
19671 | | Position of copy of COPI_cyto_for_J4027_v1 A (#25.1) relative to |
19672 | | W20_J3987_consensus_resampled.mrc (#4) coordinates: |
19673 | | Matrix rotation and translation |
19674 | | -0.38689637 -0.90569538 -0.17328327 278.18188435 |
19675 | | -0.79467842 0.23216071 0.56088111 163.52034472 |
19676 | | -0.46775786 0.35470734 -0.80955871 239.74100561 |
19677 | | Axis -0.54800188 0.78270203 0.29507876 |
19678 | | Axis point 210.04111237 0.00000000 70.64408205 |
19679 | | Rotation angle (degrees) 169.15725379 |
19680 | | Shift along axis 46.28598736 |
19681 | | |
19682 | | |
19683 | | > fitmap #25.2 inMap #4 |
19684 | | |
19685 | | Fit molecule copy of COPI_cyto_for_J4027_v1 B (#25.2) to map |
19686 | | W20_J3987_consensus_resampled.mrc (#4) using 2426 atoms |
19687 | | average map value = 0.3153, steps = 72 |
19688 | | shifted from previous position = 0.757 |
19689 | | rotated from previous position = 2.26 degrees |
19690 | | atoms outside contour = 839, contour level = 0.27665 |
19691 | | |
19692 | | Position of copy of COPI_cyto_for_J4027_v1 B (#25.2) relative to |
19693 | | W20_J3987_consensus_resampled.mrc (#4) coordinates: |
19694 | | Matrix rotation and translation |
19695 | | -0.39471401 -0.89888974 -0.19025799 280.36384015 |
19696 | | -0.79142130 0.22743083 0.56738660 162.69216971 |
19697 | | -0.46674746 0.37452966 -0.80117062 238.28935068 |
19698 | | Axis -0.54506838 0.78143761 0.30373627 |
19699 | | Axis point 209.70723896 0.00000000 70.08028018 |
19700 | | Rotation angle (degrees) 169.81012722 |
19701 | | Shift along axis 46.69343412 |
19702 | | |
19703 | | |
19704 | | > fitmap #25.3 inMap #4 |
19705 | | |
19706 | | Fit molecule copy of COPI_cyto_for_J4027_v1 C (#25.3) to map |
19707 | | W20_J3987_consensus_resampled.mrc (#4) using 1612 atoms |
19708 | | average map value = 0.3176, steps = 56 |
19709 | | shifted from previous position = 0.728 |
19710 | | rotated from previous position = 2.04 degrees |
19711 | | atoms outside contour = 641, contour level = 0.27665 |
19712 | | |
19713 | | Position of copy of COPI_cyto_for_J4027_v1 C (#25.3) relative to |
19714 | | W20_J3987_consensus_resampled.mrc (#4) coordinates: |
19715 | | Matrix rotation and translation |
19716 | | -0.39605343 -0.89988782 -0.18260232 278.68660008 |
19717 | | -0.79444663 0.23610267 0.55956242 163.22266002 |
19718 | | -0.46043050 0.36668441 -0.80842211 238.93411170 |
19719 | | Axis -0.54443563 0.78422400 0.29762824 |
19720 | | Axis point 208.79769571 0.00000000 71.16300785 |
19721 | | Rotation angle (degrees) 169.79703161 |
19722 | | Shift along axis 47.38975067 |
19723 | | |
19724 | | |
19725 | | > fitmap #25.4 inMap #4 |
19726 | | |
19727 | | Fit molecule copy of COPI_cyto_for_J4027_v1 D (#25.4) to map |
19728 | | W20_J3987_consensus_resampled.mrc (#4) using 4745 atoms |
19729 | | average map value = 0.3393, steps = 64 |
19730 | | shifted from previous position = 0.197 |
19731 | | rotated from previous position = 0.999 degrees |
19732 | | atoms outside contour = 1721, contour level = 0.27665 |
19733 | | |
19734 | | Position of copy of COPI_cyto_for_J4027_v1 D (#25.4) relative to |
19735 | | W20_J3987_consensus_resampled.mrc (#4) coordinates: |
19736 | | Matrix rotation and translation |
19737 | | -0.36755215 -0.91697806 -0.15510211 274.92468941 |
19738 | | -0.80291601 0.22872446 0.55046437 165.41983596 |
19739 | | -0.46928810 0.32685833 -0.82032452 243.67714165 |
19740 | | Axis -0.55602986 0.78127043 0.28363236 |
19741 | | Axis point 211.32871948 0.00000000 72.29578138 |
19742 | | Rotation angle (degrees) 168.40024254 |
19743 | | Shift along axis 45.48601304 |
19744 | | |
19745 | | |
19746 | | > fitmap #25.5 inMap #4 |
19747 | | |
19748 | | Fit molecule copy of COPI_cyto_for_J4027_v1 E (#25.5) to map |
19749 | | W20_J3987_consensus_resampled.mrc (#4) using 1876 atoms |
19750 | | average map value = 0.3141, steps = 48 |
19751 | | shifted from previous position = 0.53 |
19752 | | rotated from previous position = 0.888 degrees |
19753 | | atoms outside contour = 674, contour level = 0.27665 |
19754 | | |
19755 | | Position of copy of COPI_cyto_for_J4027_v1 E (#25.5) relative to |
19756 | | W20_J3987_consensus_resampled.mrc (#4) coordinates: |
19757 | | Matrix rotation and translation |
19758 | | -0.38334136 -0.90913799 -0.16284208 277.17803202 |
19759 | | -0.79171318 0.23265338 0.56485632 162.82482750 |
19760 | | -0.47564657 0.34545700 -0.80896218 242.10131222 |
19761 | | Axis -0.54889072 0.78257074 0.29377205 |
19762 | | Axis point 210.42677765 0.00000000 70.96904172 |
19763 | | Rotation angle (degrees) 168.47140268 |
19764 | | Shift along axis 46.40409379 |
19765 | | |
19766 | | |
19767 | | > fitmap #25.6 inMap #4 |
19768 | | |
19769 | | Fit molecule copy of COPI_cyto_for_J4027_v1 F (#25.6) to map |
19770 | | W20_J3987_consensus_resampled.mrc (#4) using 2400 atoms |
19771 | | average map value = 0.2626, steps = 84 |
19772 | | shifted from previous position = 2.71 |
19773 | | rotated from previous position = 2.99 degrees |
19774 | | atoms outside contour = 1310, contour level = 0.27665 |
19775 | | |
19776 | | Position of copy of COPI_cyto_for_J4027_v1 F (#25.6) relative to |
19777 | | W20_J3987_consensus_resampled.mrc (#4) coordinates: |
19778 | | Matrix rotation and translation |
19779 | | -0.40199255 -0.90304968 -0.15133829 274.52161881 |
19780 | | -0.76596507 0.24109454 0.59596221 157.65702153 |
19781 | | -0.50169664 0.35549220 -0.78862272 240.80158217 |
19782 | | Axis -0.53855753 0.78466390 0.30701522 |
19783 | | Axis point 207.61107533 0.00000000 67.84938526 |
19784 | | Rotation angle (degrees) 167.09978512 |
19785 | | Shift along axis 49.79183984 |
19786 | | |
19787 | | |
19788 | | > fitmap #25.7 inMap #4 |
19789 | | |
19790 | | Fit molecule copy of COPI_cyto_for_J4027_v1 G (#25.7) to map |
19791 | | W20_J3987_consensus_resampled.mrc (#4) using 2302 atoms |
19792 | | average map value = 0.2759, steps = 60 |
19793 | | shifted from previous position = 1.78 |
19794 | | rotated from previous position = 1.99 degrees |
19795 | | atoms outside contour = 1124, contour level = 0.27665 |
19796 | | |
19797 | | Position of copy of COPI_cyto_for_J4027_v1 G (#25.7) relative to |
19798 | | W20_J3987_consensus_resampled.mrc (#4) coordinates: |
19799 | | Matrix rotation and translation |
19800 | | -0.37472415 -0.90717861 -0.19133425 277.62448174 |
19801 | | -0.79104565 0.20520230 0.57631485 166.08473990 |
19802 | | -0.48355827 0.36731321 -0.79451395 238.83413932 |
19803 | | Axis -0.55353568 0.77394812 0.30757528 |
19804 | | Axis point 211.98709934 0.00000000 67.68536340 |
19805 | | Rotation angle (degrees) 169.11794633 |
19806 | | Shift along axis 48.32539250 |
19807 | | |
19808 | | |
19809 | | > fitmap #25.8 inMap #4 |
19810 | | |
19811 | | Fit molecule copy of COPI_cyto_for_J4027_v1 H (#25.8) to map |
19812 | | W20_J3987_consensus_resampled.mrc (#4) using 420 atoms |
19813 | | average map value = 0.339, steps = 56 |
19814 | | shifted from previous position = 1.73 |
19815 | | rotated from previous position = 4.37 degrees |
19816 | | atoms outside contour = 139, contour level = 0.27665 |
19817 | | |
19818 | | Position of copy of COPI_cyto_for_J4027_v1 H (#25.8) relative to |
19819 | | W20_J3987_consensus_resampled.mrc (#4) coordinates: |
19820 | | Matrix rotation and translation |
19821 | | -0.30262332 -0.93808653 -0.16856100 274.79297984 |
19822 | | -0.83162161 0.17348487 0.52754953 176.28282750 |
19823 | | -0.46564432 0.29982776 -0.83263359 248.60795718 |
19824 | | Axis -0.58515271 0.76338380 0.27357170 |
19825 | | Axis point 220.21870033 0.00000000 73.46391590 |
19826 | | Rotation angle (degrees) 168.77962388 |
19827 | | Shift along axis 41.78769794 |
19828 | | |
19829 | | |
19830 | | > fitmap #25.9 inMap #4 |
19831 | | |
19832 | | Fit molecule copy of COPI_cyto_for_J4027_v1 I (#25.9) to map |
19833 | | W20_J3987_consensus_resampled.mrc (#4) using 1573 atoms |
19834 | | average map value = 0.3297, steps = 60 |
19835 | | shifted from previous position = 0.384 |
19836 | | rotated from previous position = 0.597 degrees |
19837 | | atoms outside contour = 540, contour level = 0.27665 |
19838 | | |
19839 | | Position of copy of COPI_cyto_for_J4027_v1 I (#25.9) relative to |
19840 | | W20_J3987_consensus_resampled.mrc (#4) coordinates: |
19841 | | Matrix rotation and translation |
19842 | | -0.37451788 -0.91088738 -0.17326443 276.29637371 |
19843 | | -0.79727848 0.22096017 0.56171491 164.46884657 |
19844 | | -0.47337448 0.34851227 -0.80898443 241.62397254 |
19845 | | Axis -0.55339354 0.77897237 0.29488579 |
19846 | | Axis point 210.79260550 0.00000000 70.83395311 |
19847 | | Rotation angle (degrees) 168.89357390 |
19848 | | Shift along axis 46.46753453 |
19849 | | |
19850 | | |
19851 | | > fitmap #25.10 inMap #4 |
19852 | | |
19853 | | Fit molecule copy of COPI_cyto_for_J4027_v1 J (#25.10) to map |
19854 | | W20_J3987_consensus_resampled.mrc (#4) using 1112 atoms |
19855 | | average map value = 0.3174, steps = 56 |
19856 | | shifted from previous position = 0.808 |
19857 | | rotated from previous position = 4.24 degrees |
19858 | | atoms outside contour = 445, contour level = 0.27665 |
19859 | | |
19860 | | Position of copy of COPI_cyto_for_J4027_v1 J (#25.10) relative to |
19861 | | W20_J3987_consensus_resampled.mrc (#4) coordinates: |
19862 | | Matrix rotation and translation |
19863 | | -0.37184146 -0.92295368 -0.09945074 269.60235343 |
19864 | | -0.79986239 0.26418072 0.53891439 164.53018199 |
19865 | | -0.47112005 0.27993762 -0.83646927 249.12759996 |
19866 | | Axis -0.55169100 0.79175677 0.26221794 |
19867 | | Axis point 209.92414105 0.00000000 75.11235729 |
19868 | | Rotation angle (degrees) 166.42536541 |
19869 | | Shift along axis 46.85641909 |
19870 | | |
19871 | | |
19872 | | > fitmap #25.11 inMap #4 |
19873 | | |
19874 | | Fit molecule copy of COPI_cyto_for_J4027_v1 K (#25.11) to map |
19875 | | W20_J3987_consensus_resampled.mrc (#4) using 319 atoms |
19876 | | average map value = 0.3247, steps = 356 |
19877 | | shifted from previous position = 18 |
19878 | | rotated from previous position = 29.8 degrees |
19879 | | atoms outside contour = 122, contour level = 0.27665 |
19880 | | |
19881 | | Position of copy of COPI_cyto_for_J4027_v1 K (#25.11) relative to |
19882 | | W20_J3987_consensus_resampled.mrc (#4) coordinates: |
19883 | | Matrix rotation and translation |
19884 | | -0.11497818 -0.99056474 0.07457564 250.16493380 |
19885 | | -0.55287660 0.12618381 0.82365352 113.16758699 |
19886 | | -0.82529237 0.05347106 -0.56216845 259.79352847 |
19887 | | Axis -0.60992918 0.71263081 0.34661756 |
19888 | | Axis point 236.76411941 0.00000000 37.20193482 |
19889 | | Rotation angle (degrees) 140.84869902 |
19890 | | Shift along axis 18.11281583 |
19891 | | |
19892 | | |
19893 | | > fitmap #25.12 inMap #4 |
19894 | | |
19895 | | Fit molecule copy of COPI_cyto_for_J4027_v1 L (#25.12) to map |
19896 | | W20_J3987_consensus_resampled.mrc (#4) using 1420 atoms |
19897 | | average map value = 0.2216, steps = 244 |
19898 | | shifted from previous position = 20 |
19899 | | rotated from previous position = 43.1 degrees |
19900 | | atoms outside contour = 1209, contour level = 0.27665 |
19901 | | |
19902 | | Position of copy of COPI_cyto_for_J4027_v1 L (#25.12) relative to |
19903 | | W20_J3987_consensus_resampled.mrc (#4) coordinates: |
19904 | | Matrix rotation and translation |
19905 | | -0.33704271 -0.93889900 0.06979181 244.25606438 |
19906 | | -0.21172527 0.14781713 0.96608619 12.69558044 |
19907 | | -0.91737377 0.31083561 -0.24860932 233.11919004 |
19908 | | Axis -0.47133855 0.71009352 0.52307472 |
19909 | | Axis point 190.36197930 0.00000000 40.21086330 |
19910 | | Rotation angle (degrees) 135.96517505 |
19911 | | Shift along axis 15.82650607 |
19912 | | |
19913 | | |
19914 | | > close #25 |
19915 | | |
19916 | | > combine #28 |
19917 | | |
19918 | | > color #4 darkgrey models |
19919 | | |
19920 | | > color #4 #a9a9a9bf models |
19921 | | |
19922 | | > rename #25 COPI_cyto_J3987_model |
19923 | | |
19924 | | > fitmap #25 inMap #4 |
19925 | | |
19926 | | Fit molecule COPI_cyto_J3987_model (#25) to map |
19927 | | W20_J3987_consensus_resampled.mrc (#4) using 27347 atoms |
19928 | | average map value = 0.2864, steps = 40 |
19929 | | shifted from previous position = 0.228 |
19930 | | rotated from previous position = 0.219 degrees |
19931 | | atoms outside contour = 13455, contour level = 0.27665 |
19932 | | |
19933 | | Position of COPI_cyto_J3987_model (#25) relative to |
19934 | | W20_J3987_consensus_resampled.mrc (#4) coordinates: |
19935 | | Matrix rotation and translation |
19936 | | -0.37179442 -0.91394156 -0.16272598 275.88087564 |
19937 | | -0.79687792 0.22429417 0.56096143 164.32455600 |
19938 | | -0.47618747 0.33823507 -0.81169116 242.44716299 |
19939 | | Axis -0.55410562 0.77983932 0.29123460 |
19940 | | Axis point 211.23166983 0.00000000 70.92651391 |
19941 | | Rotation angle (degrees) 168.40582967 |
19942 | | Shift along axis 45.88860967 |
19943 | | |
19944 | | |
19945 | | > hide #!25 models |
19946 | | |
19947 | | > show #!24 models |
19948 | | |
19949 | | > combine #28 |
19950 | | |
19951 | | > hide #!4 models |
19952 | | |
19953 | | > show #!6 models |
19954 | | |
19955 | | > color #6 darkgrey models |
19956 | | |
19957 | | > color #6 #a9a9a9bf models |
19958 | | |
19959 | | > fitmap #26 inMap #6 |
19960 | | |
19961 | | Fit molecule copy of COPI_cyto_for_J4027_v1 (#26) to map |
19962 | | W20_J4021_consensus_resampled.mrc (#6) using 27347 atoms |
19963 | | average map value = 0.2574, steps = 88 |
19964 | | shifted from previous position = 0.804 |
19965 | | rotated from previous position = 1.33 degrees |
19966 | | atoms outside contour = 13999, contour level = 0.25941 |
19967 | | |
19968 | | Position of copy of COPI_cyto_for_J4027_v1 (#26) relative to |
19969 | | W20_J4021_consensus_resampled.mrc (#6) coordinates: |
19970 | | Matrix rotation and translation |
19971 | | -0.39044300 -0.90597356 -0.16360374 277.32294036 |
19972 | | -0.78887242 0.23762979 0.56675603 162.18357201 |
19973 | | -0.47458885 0.35034840 -0.80747844 241.34338581 |
19974 | | Axis -0.54571767 0.78421483 0.29529532 |
19975 | | Axis point 209.61127190 0.00000000 70.79010036 |
19976 | | Rotation angle (degrees) 168.56372015 |
19977 | | Shift along axis 47.11430470 |
19978 | | |
19979 | | |
19980 | | > rename #26 COPI_cyto_J4021_model |
19981 | | |
19982 | | > hide #!26 models |
19983 | | |
19984 | | > combine #28 |
19985 | | |
19986 | | > close #29-33 |
19987 | | |
19988 | | > rename #27 COPI_cyto_J4022_model |
19989 | | |
19990 | | > rename #28 COPI_cyto_J4023_model |
19991 | | |
19992 | | > combine #28 |
19993 | | |
19994 | | > hide #!27 models |
19995 | | |
19996 | | > hide #!24 models |
19997 | | |
19998 | | > rename #29 COPI_cyto_J4024_model |
19999 | | |
20000 | | > combine #29 |
20001 | | |
20002 | | [Repeated 1 time(s)] |
20003 | | |
20004 | | > rename #30 COPI_cyto_J4025_model |
20005 | | |
20006 | | > rename #31 COPI_cyto_J4026_model |
20007 | | |
20008 | | > combine #29 |
20009 | | |
20010 | | > rename #32 COPI_cyto_J4027_model |
20011 | | |
20012 | | > combine #29 |
20013 | | |
20014 | | > rename #33 COPI_cyto_J4028_model |
20015 | | |
20016 | | > hide #!29 models |
20017 | | |
20018 | | > hide #!30 models |
20019 | | |
20020 | | > hide #!31 models |
20021 | | |
20022 | | > hide #!32 models |
20023 | | |
20024 | | > hide #!33 models |
20025 | | |
20026 | | > combine #29 |
20027 | | |
20028 | | > close #35-36,39#34,37-38 |
20029 | | |
20030 | | > combine #29 |
20031 | | |
20032 | | > rename #34 COPI_cyto_J3988_model |
20033 | | |
20034 | | > hide #!34 models |
20035 | | |
20036 | | > close #40 |
20037 | | |
20038 | | > close #41 |
20039 | | |
20040 | | > close #42-46 |
20041 | | |
20042 | | > hide #!6 models |
20043 | | |
20044 | | > show #!6 models |
20045 | | |
20046 | | > hide #!6 models |
20047 | | |
20048 | | > show #!1 models |
20049 | | |
20050 | | > fitmap #24.1 inMap #1 |
20051 | | |
20052 | | Fit molecule COPI_cyto_for_J4027_v1 A (#24.1) to map |
20053 | | W20_J3623_consensus_structure.mrc (#1) using 2590 atoms |
20054 | | average map value = 0.3549, steps = 116 |
20055 | | shifted from previous position = 0.529 |
20056 | | rotated from previous position = 1.66 degrees |
20057 | | atoms outside contour = 606, contour level = 0.25746 |
20058 | | |
20059 | | Position of COPI_cyto_for_J4027_v1 A (#24.1) relative to |
20060 | | W20_J3623_consensus_structure.mrc (#1) coordinates: |
20061 | | Matrix rotation and translation |
20062 | | -0.39542896 -0.90304867 -0.16774700 278.17983361 |
20063 | | -0.78821131 0.23986960 0.56673233 161.55778287 |
20064 | | -0.47154946 0.35632245 -0.80664455 240.52362588 |
20065 | | Axis -0.54371860 0.78505369 0.29675038 |
20066 | | Axis point 209.13140963 0.00000000 70.83505847 |
20067 | | Rotation angle (degrees) 168.84338449 |
20068 | | Shift along axis 46.95546224 |
20069 | | |
20070 | | |
20071 | | > fitmap #24.2 inMap #1 |
20072 | | |
20073 | | Fit molecule COPI_cyto_for_J4027_v1 B (#24.2) to map |
20074 | | W20_J3623_consensus_structure.mrc (#1) using 2426 atoms |
20075 | | average map value = 0.3443, steps = 84 |
20076 | | shifted from previous position = 1.05 |
20077 | | rotated from previous position = 2.23 degrees |
20078 | | atoms outside contour = 613, contour level = 0.25746 |
20079 | | |
20080 | | Position of COPI_cyto_for_J4027_v1 B (#24.2) relative to |
20081 | | W20_J3623_consensus_structure.mrc (#1) coordinates: |
20082 | | Matrix rotation and translation |
20083 | | -0.39499091 -0.89904452 -0.18894748 279.87559115 |
20084 | | -0.79097752 0.22820260 0.56769548 163.08646468 |
20085 | | -0.46726520 0.37368776 -0.80126194 238.60471247 |
20086 | | Axis -0.54487475 0.78166114 0.30350844 |
20087 | | Axis point 209.64905720 0.00000000 70.09351394 |
20088 | | Rotation angle (degrees) 169.74498652 |
20089 | | Shift along axis 47.39975428 |
20090 | | |
20091 | | |
20092 | | > fitmap #24.3 inMap #1 |
20093 | | |
20094 | | Fit molecule COPI_cyto_for_J4027_v1 C (#24.3) to map |
20095 | | W20_J3623_consensus_structure.mrc (#1) using 1612 atoms |
20096 | | average map value = 0.3398, steps = 68 |
20097 | | shifted from previous position = 0.582 |
20098 | | rotated from previous position = 1.57 degrees |
20099 | | atoms outside contour = 545, contour level = 0.25746 |
20100 | | |
20101 | | Position of COPI_cyto_for_J4027_v1 C (#24.3) relative to |
20102 | | W20_J3623_consensus_structure.mrc (#1) coordinates: |
20103 | | Matrix rotation and translation |
20104 | | -0.38289473 -0.90536973 -0.18356823 277.48749747 |
20105 | | -0.80205073 0.22720768 0.55235071 164.98384273 |
20106 | | -0.45837350 0.35872321 -0.81314907 239.04481293 |
20107 | | Axis -0.55056634 0.78138966 0.29378038 |
20108 | | Axis point 209.64934014 0.00000000 71.32622789 |
20109 | | Rotation angle (degrees) 169.87222630 |
20110 | | Shift along axis 46.36806823 |
20111 | | |
20112 | | |
20113 | | > fitmap #24.4 inMap #1 |
20114 | | |
20115 | | Fit molecule COPI_cyto_for_J4027_v1 D (#24.4) to map |
20116 | | W20_J3623_consensus_structure.mrc (#1) using 4745 atoms |
20117 | | average map value = 0.3686, steps = 104 |
20118 | | shifted from previous position = 0.253 |
20119 | | rotated from previous position = 1.26 degrees |
20120 | | atoms outside contour = 1389, contour level = 0.25746 |
20121 | | |
20122 | | Position of COPI_cyto_for_J4027_v1 D (#24.4) relative to |
20123 | | W20_J3623_consensus_structure.mrc (#1) coordinates: |
20124 | | Matrix rotation and translation |
20125 | | -0.36437524 -0.91895662 -0.15082912 274.51036554 |
20126 | | -0.80384056 0.22859535 0.54916713 165.68273324 |
20127 | | -0.47018193 0.32134547 -0.82198909 244.27756191 |
20128 | | Axis -0.55725502 0.78114152 0.28157552 |
20129 | | Axis point 211.66245571 0.00000000 72.46008698 |
20130 | | Rotation angle (degrees) 168.20479189 |
20131 | | Shift along axis 45.23196284 |
20132 | | |
20133 | | |
20134 | | > fitmap #24.5 inMap #1 |
20135 | | |
20136 | | Fit molecule COPI_cyto_for_J4027_v1 E (#24.5) to map |
20137 | | W20_J3623_consensus_structure.mrc (#1) using 1876 atoms |
20138 | | average map value = 0.352, steps = 52 |
20139 | | shifted from previous position = 0.551 |
20140 | | rotated from previous position = 2.49 degrees |
20141 | | atoms outside contour = 518, contour level = 0.25746 |
20142 | | |
20143 | | Position of COPI_cyto_for_J4027_v1 E (#24.5) relative to |
20144 | | W20_J3623_consensus_structure.mrc (#1) coordinates: |
20145 | | Matrix rotation and translation |
20146 | | -0.40106793 -0.89989636 -0.17126318 279.30609803 |
20147 | | -0.77603258 0.23443438 0.58550318 159.37835572 |
20148 | | -0.48674220 0.36773235 -0.79237300 240.44712243 |
20149 | | Axis -0.54056671 0.78310515 0.30746375 |
20150 | | Axis point 209.32932986 0.00000000 69.11212793 |
20151 | | Rotation angle (degrees) 168.37950772 |
20152 | | Shift along axis 47.75520439 |
20153 | | |
20154 | | |
20155 | | > fitmap #24.6 inMap #1 |
20156 | | |
20157 | | Fit molecule COPI_cyto_for_J4027_v1 F (#24.6) to map |
20158 | | W20_J3623_consensus_structure.mrc (#1) using 2400 atoms |
20159 | | average map value = 0.1995, steps = 32 |
20160 | | shifted from previous position = 0.88 |
20161 | | rotated from previous position = 2.72 degrees |
20162 | | atoms outside contour = 2236, contour level = 0.25746 |
20163 | | |
20164 | | Position of COPI_cyto_for_J4027_v1 F (#24.6) relative to |
20165 | | W20_J3623_consensus_structure.mrc (#1) coordinates: |
20166 | | Matrix rotation and translation |
20167 | | -0.34705513 -0.92807037 -0.13504869 272.53895474 |
20168 | | -0.78593629 0.20923862 0.58182761 162.32498204 |
20169 | | -0.51171956 0.30806592 -0.80202151 247.95535590 |
20170 | | Axis -0.56230557 0.77368067 0.29194291 |
20171 | | Axis point 214.01677259 0.00000000 69.57811076 |
20172 | | Rotation angle (degrees) 165.91105545 |
20173 | | Shift along axis 44.72633565 |
20174 | | |
20175 | | |
20176 | | > fitmap #24.7 inMap #1 |
20177 | | |
20178 | | Fit molecule COPI_cyto_for_J4027_v1 G (#24.7) to map |
20179 | | W20_J3623_consensus_structure.mrc (#1) using 2302 atoms |
20180 | | average map value = 0.2042, steps = 56 |
20181 | | shifted from previous position = 0.732 |
20182 | | rotated from previous position = 2.42 degrees |
20183 | | atoms outside contour = 1956, contour level = 0.25746 |
20184 | | |
20185 | | Position of COPI_cyto_for_J4027_v1 G (#24.7) relative to |
20186 | | W20_J3623_consensus_structure.mrc (#1) coordinates: |
20187 | | Matrix rotation and translation |
20188 | | -0.40089736 -0.90460310 -0.14482596 274.53357578 |
20189 | | -0.79035024 0.26156664 0.55401210 159.12795064 |
20190 | | -0.46327942 0.33656522 -0.81981464 242.17863151 |
20191 | | Axis -0.54066936 0.79181649 0.28408325 |
20192 | | Axis point 206.28449602 0.00000000 73.32567888 |
20193 | | Rotation angle (degrees) 168.39926749 |
20194 | | Shift along axis 46.36713644 |
20195 | | |
20196 | | |
20197 | | > fitmap #24.8 inMap #1 |
20198 | | |
20199 | | Fit molecule COPI_cyto_for_J4027_v1 H (#24.8) to map |
20200 | | W20_J3623_consensus_structure.mrc (#1) using 420 atoms |
20201 | | average map value = 0.2665, steps = 72 |
20202 | | shifted from previous position = 1.63 |
20203 | | rotated from previous position = 6.63 degrees |
20204 | | atoms outside contour = 172, contour level = 0.25746 |
20205 | | |
20206 | | Position of COPI_cyto_for_J4027_v1 H (#24.8) relative to |
20207 | | W20_J3623_consensus_structure.mrc (#1) coordinates: |
20208 | | Matrix rotation and translation |
20209 | | -0.32134690 -0.93539737 -0.14753964 274.57265625 |
20210 | | -0.85481390 0.21949425 0.47022919 174.99288921 |
20211 | | -0.40746704 0.27722563 -0.87012446 247.94129870 |
20212 | | Axis -0.57850272 0.77909809 0.24153833 |
20213 | | Axis point 215.13355776 0.00000000 80.87806975 |
20214 | | Rotation angle (degrees) 170.39742774 |
20215 | | Shift along axis 37.38292387 |
20216 | | |
20217 | | |
20218 | | > fitmap #24.9 inMap #1 |
20219 | | |
20220 | | Fit molecule COPI_cyto_for_J4027_v1 I (#24.9) to map |
20221 | | W20_J3623_consensus_structure.mrc (#1) using 1573 atoms |
20222 | | average map value = 0.3464, steps = 64 |
20223 | | shifted from previous position = 0.478 |
20224 | | rotated from previous position = 0.943 degrees |
20225 | | atoms outside contour = 463, contour level = 0.25746 |
20226 | | |
20227 | | Position of COPI_cyto_for_J4027_v1 I (#24.9) relative to |
20228 | | W20_J3623_consensus_structure.mrc (#1) coordinates: |
20229 | | Matrix rotation and translation |
20230 | | -0.36293127 -0.91488242 -0.17683625 275.64058476 |
20231 | | -0.80647349 0.21333854 0.55144102 166.30863857 |
20232 | | -0.46677771 0.34274894 -0.81525564 240.90103613 |
20233 | | Axis -0.55901039 0.77664797 0.29038821 |
20234 | | Axis point 211.48290973 0.00000000 71.12426755 |
20235 | | Rotation angle (degrees) 169.24194914 |
20236 | | Shift along axis 45.03213770 |
20237 | | |
20238 | | |
20239 | | > fitmap #24.10 inMap #1 |
20240 | | |
20241 | | Fit molecule COPI_cyto_for_J4027_v1 J (#24.10) to map |
20242 | | W20_J3623_consensus_structure.mrc (#1) using 1112 atoms |
20243 | | average map value = 0.3459, steps = 52 |
20244 | | shifted from previous position = 0.38 |
20245 | | rotated from previous position = 3.4 degrees |
20246 | | atoms outside contour = 351, contour level = 0.25746 |
20247 | | |
20248 | | Position of COPI_cyto_for_J4027_v1 J (#24.10) relative to |
20249 | | W20_J3623_consensus_structure.mrc (#1) coordinates: |
20250 | | Matrix rotation and translation |
20251 | | -0.36982106 -0.92265040 -0.10930983 270.39934908 |
20252 | | -0.79564879 0.25374928 0.55004938 163.22079540 |
20253 | | -0.47976598 0.29039208 -0.82794750 248.56734521 |
20254 | | Axis -0.55260110 0.78840254 0.27028405 |
20255 | | Axis point 210.23381150 0.00000000 73.93598950 |
20256 | | Rotation angle (degrees) 166.41185550 |
20257 | | Shift along axis 46.44450154 |
20258 | | |
20259 | | |
20260 | | > fitmap #24.11 inMap #1 |
20261 | | |
20262 | | Fit molecule COPI_cyto_for_J4027_v1 K (#24.11) to map |
20263 | | W20_J3623_consensus_structure.mrc (#1) using 319 atoms |
20264 | | average map value = 0.2981, steps = 324 |
20265 | | shifted from previous position = 19.5 |
20266 | | rotated from previous position = 32.5 degrees |
20267 | | atoms outside contour = 138, contour level = 0.25746 |
20268 | | |
20269 | | Position of COPI_cyto_for_J4027_v1 K (#24.11) relative to |
20270 | | W20_J3623_consensus_structure.mrc (#1) coordinates: |
20271 | | Matrix rotation and translation |
20272 | | -0.06351642 -0.99614584 0.06049082 250.07246282 |
20273 | | -0.53379297 0.08512482 0.84131971 112.08368631 |
20274 | | -0.84322639 0.02114804 -0.53714247 260.93738590 |
20275 | | Axis -0.62845977 0.69247687 0.35427971 |
20276 | | Axis point 244.61405927 0.00000000 32.59441219 |
20277 | | Rotation angle (degrees) 139.26773764 |
20278 | | Shift along axis 12.89969989 |
20279 | | |
20280 | | |
20281 | | > split #25 chains |
20282 | | |
20283 | | Split COPI_cyto_J3987_model (#25) into 13 models |
20284 | | Chain information for COPI_cyto_J3987_model A #25.1 |
20285 | | --- |
20286 | | Chain | Description |
20287 | | A | No description available |
20288 | | |
20289 | | Chain information for COPI_cyto_J3987_model B #25.2 |
20290 | | --- |
20291 | | Chain | Description |
20292 | | B | No description available |
20293 | | |
20294 | | Chain information for COPI_cyto_J3987_model C #25.3 |
20295 | | --- |
20296 | | Chain | Description |
20297 | | C | No description available |
20298 | | |
20299 | | Chain information for COPI_cyto_J3987_model D #25.4 |
20300 | | --- |
20301 | | Chain | Description |
20302 | | D | No description available |
20303 | | |
20304 | | Chain information for COPI_cyto_J3987_model E #25.5 |
20305 | | --- |
20306 | | Chain | Description |
20307 | | E | No description available |
20308 | | |
20309 | | Chain information for COPI_cyto_J3987_model F #25.6 |
20310 | | --- |
20311 | | Chain | Description |
20312 | | F | No description available |
20313 | | |
20314 | | Chain information for COPI_cyto_J3987_model G #25.7 |
20315 | | --- |
20316 | | Chain | Description |
20317 | | G | No description available |
20318 | | |
20319 | | Chain information for COPI_cyto_J3987_model H #25.8 |
20320 | | --- |
20321 | | Chain | Description |
20322 | | H | No description available |
20323 | | |
20324 | | Chain information for COPI_cyto_J3987_model I #25.9 |
20325 | | --- |
20326 | | Chain | Description |
20327 | | I | No description available |
20328 | | |
20329 | | Chain information for COPI_cyto_J3987_model J #25.10 |
20330 | | --- |
20331 | | Chain | Description |
20332 | | J | No description available |
20333 | | |
20334 | | Chain information for COPI_cyto_J3987_model K #25.11 |
20335 | | --- |
20336 | | Chain | Description |
20337 | | K | No description available |
20338 | | |
20339 | | Chain information for COPI_cyto_J3987_model L #25.12 |
20340 | | --- |
20341 | | Chain | Description |
20342 | | L | No description available |
20343 | | |
20344 | | Chain information for COPI_cyto_J3987_model M #25.13 |
20345 | | --- |
20346 | | Chain | Description |
20347 | | M | No description available |
20348 | | |
20349 | | |
20350 | | > hide #!1 models |
20351 | | |
20352 | | > show #!4 models |
20353 | | |
20354 | | > fitmap #25.1 inMap #4 |
20355 | | |
20356 | | Fit molecule COPI_cyto_J3987_model A (#25.1) to map |
20357 | | W20_J3987_consensus_resampled.mrc (#4) using 2590 atoms |
20358 | | average map value = 0.3221, steps = 80 |
20359 | | shifted from previous position = 0.776 |
20360 | | rotated from previous position = 1.16 degrees |
20361 | | atoms outside contour = 793, contour level = 0.27665 |
20362 | | |
20363 | | Position of COPI_cyto_J3987_model A (#25.1) relative to |
20364 | | W20_J3987_consensus_resampled.mrc (#4) coordinates: |
20365 | | Matrix rotation and translation |
20366 | | -0.38698629 -0.90565681 -0.17328409 278.18136001 |
20367 | | -0.79448095 0.23210684 0.56118308 163.47572576 |
20368 | | -0.46801885 0.35484106 -0.80934924 239.73459315 |
20369 | | Axis -0.54794991 0.78268057 0.29523216 |
20370 | | Axis point 210.03344096 0.00000000 70.61465245 |
20371 | | Rotation angle (degrees) 169.14725684 |
20372 | | Shift along axis 46.29718384 |
20373 | | |
20374 | | |
20375 | | > fitmap #25.2 inMap #4 |
20376 | | |
20377 | | Fit molecule COPI_cyto_J3987_model B (#25.2) to map |
20378 | | W20_J3987_consensus_resampled.mrc (#4) using 2426 atoms |
20379 | | average map value = 0.3153, steps = 72 |
20380 | | shifted from previous position = 0.76 |
20381 | | rotated from previous position = 2.24 degrees |
20382 | | atoms outside contour = 837, contour level = 0.27665 |
20383 | | |
20384 | | Position of COPI_cyto_J3987_model B (#25.2) relative to |
20385 | | W20_J3987_consensus_resampled.mrc (#4) coordinates: |
20386 | | Matrix rotation and translation |
20387 | | -0.39446310 -0.89902146 -0.19015597 280.33015308 |
20388 | | -0.79147162 0.22726378 0.56738336 162.70699196 |
20389 | | -0.46687424 0.37431485 -0.80119714 238.32585966 |
20390 | | Axis -0.54517505 0.78138014 0.30369267 |
20391 | | Axis point 209.72584131 0.00000000 70.08251673 |
20392 | | Rotation angle (degrees) 169.80084660 |
20393 | | Shift along axis 46.68482501 |
20394 | | |
20395 | | |
20396 | | > fitmap #25.3 inMap #4 |
20397 | | |
20398 | | Fit molecule COPI_cyto_J3987_model C (#25.3) to map |
20399 | | W20_J3987_consensus_resampled.mrc (#4) using 1612 atoms |
20400 | | average map value = 0.3176, steps = 88 |
20401 | | shifted from previous position = 0.713 |
20402 | | rotated from previous position = 1.99 degrees |
20403 | | atoms outside contour = 638, contour level = 0.27665 |
20404 | | |
20405 | | Position of COPI_cyto_J3987_model C (#25.3) relative to |
20406 | | W20_J3987_consensus_resampled.mrc (#4) coordinates: |
20407 | | Matrix rotation and translation |
20408 | | -0.39593352 -0.89983916 -0.18310146 278.71276949 |
20409 | | -0.79448375 0.23569092 0.55968328 163.24766694 |
20410 | | -0.46046958 0.36706850 -0.80822553 238.88829135 |
20411 | | Axis -0.54450663 0.78409758 0.29783135 |
20412 | | Axis point 208.81546281 0.00000000 71.13107453 |
20413 | | Rotation angle (degrees) 169.81244748 |
20414 | | Shift along axis 47.38957070 |
20415 | | |
20416 | | |
20417 | | > fitmap #25.4 inMap #4 |
20418 | | |
20419 | | Fit molecule COPI_cyto_J3987_model D (#25.4) to map |
20420 | | W20_J3987_consensus_resampled.mrc (#4) using 4745 atoms |
20421 | | average map value = 0.3393, steps = 64 |
20422 | | shifted from previous position = 0.187 |
20423 | | rotated from previous position = 0.91 degrees |
20424 | | atoms outside contour = 1720, contour level = 0.27665 |
20425 | | |
20426 | | Position of COPI_cyto_J3987_model D (#25.4) relative to |
20427 | | W20_J3987_consensus_resampled.mrc (#4) coordinates: |
20428 | | Matrix rotation and translation |
20429 | | -0.36779496 -0.91688768 -0.15506087 274.91861534 |
20430 | | -0.80287248 0.22897373 0.55042423 165.38152260 |
20431 | | -0.46917233 0.32693736 -0.82035926 243.65468775 |
20432 | | Axis -0.55592398 0.78135279 0.28361301 |
20433 | | Axis point 211.28631778 0.00000000 72.30612718 |
20434 | | Rotation angle (degrees) 168.40427173 |
20435 | | Shift along axis 45.49110059 |
20436 | | |
20437 | | |
20438 | | > hide #!95 models |
20439 | | |
20440 | | > show #25.1 models |
20441 | | |
20442 | | > show #25.2 models |
20443 | | |
20444 | | > show #25.3 models |
20445 | | |
20446 | | > show #!25.4 models |
20447 | | |
20448 | | > show #25.5 models |
20449 | | |
20450 | | > show #25.6 models |
20451 | | |
20452 | | > show #25.7 models |
20453 | | |
20454 | | > show #25.8 models |
20455 | | |
20456 | | > show #25.9 models |
20457 | | |
20458 | | > show #25.10 models |
20459 | | |
20460 | | > show #25.11 models |
20461 | | |
20462 | | > show #25.12 models |
20463 | | |
20464 | | > show #25.13 models |
20465 | | |
20466 | | > fitmap #25.5 inMap #4 |
20467 | | |
20468 | | Fit molecule COPI_cyto_J3987_model E (#25.5) to map |
20469 | | W20_J3987_consensus_resampled.mrc (#4) using 1876 atoms |
20470 | | average map value = 0.3141, steps = 60 |
20471 | | shifted from previous position = 0.493 |
20472 | | rotated from previous position = 0.732 degrees |
20473 | | atoms outside contour = 674, contour level = 0.27665 |
20474 | | |
20475 | | Position of COPI_cyto_J3987_model E (#25.5) relative to |
20476 | | W20_J3987_consensus_resampled.mrc (#4) coordinates: |
20477 | | Matrix rotation and translation |
20478 | | -0.38331200 -0.90908551 -0.16320372 277.20038029 |
20479 | | -0.79186725 0.23250965 0.56469952 162.86818551 |
20480 | | -0.47541371 0.34569178 -0.80899877 242.05560365 |
20481 | | Axis -0.54892849 0.78253380 0.29379988 |
20482 | | Axis point 210.42960898 0.00000000 70.96821393 |
20483 | | Rotation angle (degrees) 168.49306276 |
20484 | | Shift along axis 46.40258047 |
20485 | | |
20486 | | |
20487 | | > fitmap #25.6 inMap #4 |
20488 | | |
20489 | | Fit molecule COPI_cyto_J3987_model F (#25.6) to map |
20490 | | W20_J3987_consensus_resampled.mrc (#4) using 2400 atoms |
20491 | | average map value = 0.2626, steps = 52 |
20492 | | shifted from previous position = 2.64 |
20493 | | rotated from previous position = 2.91 degrees |
20494 | | atoms outside contour = 1308, contour level = 0.27665 |
20495 | | |
20496 | | Position of COPI_cyto_J3987_model F (#25.6) relative to |
20497 | | W20_J3987_consensus_resampled.mrc (#4) coordinates: |
20498 | | Matrix rotation and translation |
20499 | | -0.40208359 -0.90303670 -0.15117378 274.51102549 |
20500 | | -0.76578539 0.24117028 0.59616243 157.61497455 |
20501 | | -0.50189793 0.35547381 -0.78850292 240.80443864 |
20502 | | Axis -0.53849728 0.78468186 0.30707499 |
20503 | | Axis point 207.60053656 0.00000000 67.83219255 |
20504 | | Rotation angle (degrees) 167.08638464 |
20505 | | Shift along axis 49.79919045 |
20506 | | |
20507 | | |
20508 | | > fitmap #25.7 inMap #4 |
20509 | | |
20510 | | Fit molecule COPI_cyto_J3987_model G (#25.7) to map |
20511 | | W20_J3987_consensus_resampled.mrc (#4) using 2302 atoms |
20512 | | average map value = 0.2759, steps = 68 |
20513 | | shifted from previous position = 1.66 |
20514 | | rotated from previous position = 2.11 degrees |
20515 | | atoms outside contour = 1125, contour level = 0.27665 |
20516 | | |
20517 | | Position of COPI_cyto_J3987_model G (#25.7) relative to |
20518 | | W20_J3987_consensus_resampled.mrc (#4) coordinates: |
20519 | | Matrix rotation and translation |
20520 | | -0.37470678 -0.90717007 -0.19140875 277.62743359 |
20521 | | -0.79108106 0.20516374 0.57627998 166.08595678 |
20522 | | -0.48351381 0.36735586 -0.79452130 238.82922597 |
20523 | | Axis -0.55354811 0.77393742 0.30757985 |
20524 | | Axis point 211.98563964 0.00000000 67.68895792 |
20525 | | Rotation angle (degrees) 169.12227850 |
20526 | | Shift along axis 48.31905400 |
20527 | | |
20528 | | |
20529 | | > fitmap #25.8 inMap #4 |
20530 | | |
20531 | | Fit molecule COPI_cyto_J3987_model H (#25.8) to map |
20532 | | W20_J3987_consensus_resampled.mrc (#4) using 420 atoms |
20533 | | average map value = 0.3391, steps = 52 |
20534 | | shifted from previous position = 1.47 |
20535 | | rotated from previous position = 4.51 degrees |
20536 | | atoms outside contour = 138, contour level = 0.27665 |
20537 | | |
20538 | | Position of COPI_cyto_J3987_model H (#25.8) relative to |
20539 | | W20_J3987_consensus_resampled.mrc (#4) coordinates: |
20540 | | Matrix rotation and translation |
20541 | | -0.30273203 -0.93833428 -0.16697938 274.74649750 |
20542 | | -0.83167276 0.17451603 0.52712864 176.16874621 |
20543 | | -0.46548229 0.29845092 -0.83321864 248.80226785 |
20544 | | Axis -0.58505795 0.76370144 0.27288698 |
20545 | | Axis point 220.19027093 0.00000000 73.60291153 |
20546 | | Rotation angle (degrees) 168.73005781 |
20547 | | Shift along axis 41.69260200 |
20548 | | |
20549 | | |
20550 | | > fitmap #25.9 inMap #4 |
20551 | | |
20552 | | Fit molecule COPI_cyto_J3987_model I (#25.9) to map |
20553 | | W20_J3987_consensus_resampled.mrc (#4) using 1573 atoms |
20554 | | average map value = 0.3297, steps = 60 |
20555 | | shifted from previous position = 0.422 |
20556 | | rotated from previous position = 0.637 degrees |
20557 | | atoms outside contour = 541, contour level = 0.27665 |
20558 | | |
20559 | | Position of COPI_cyto_J3987_model I (#25.9) relative to |
20560 | | W20_J3987_consensus_resampled.mrc (#4) coordinates: |
20561 | | Matrix rotation and translation |
20562 | | -0.37486313 -0.91082017 -0.17287068 276.29172892 |
20563 | | -0.79723061 0.22153165 0.56155775 164.41773716 |
20564 | | -0.47318179 0.34832509 -0.80917775 241.63479140 |
20565 | | Axis -0.55323087 0.77915540 0.29470744 |
20566 | | Axis point 210.74543880 0.00000000 70.87207510 |
20567 | | Rotation angle (degrees) 168.88868220 |
20568 | | Shift along axis 46.46542504 |
20569 | | |
20570 | | |
20571 | | > fitmap #25.10 inMap #4 |
20572 | | |
20573 | | Fit molecule COPI_cyto_J3987_model J (#25.10) to map |
20574 | | W20_J3987_consensus_resampled.mrc (#4) using 1112 atoms |
20575 | | average map value = 0.3174, steps = 68 |
20576 | | shifted from previous position = 0.699 |
20577 | | rotated from previous position = 4.11 degrees |
20578 | | atoms outside contour = 446, contour level = 0.27665 |
20579 | | |
20580 | | Position of COPI_cyto_J3987_model J (#25.10) relative to |
20581 | | W20_J3987_consensus_resampled.mrc (#4) coordinates: |
20582 | | Matrix rotation and translation |
20583 | | -0.37167949 -0.92301396 -0.09949677 269.60077643 |
20584 | | -0.80025326 0.26421818 0.53831543 164.62224104 |
20585 | | -0.47058379 0.27970342 -0.83684940 249.14141931 |
20586 | | Axis -0.55179431 0.79177956 0.26193160 |
20587 | | Axis point 209.93718651 0.00000000 75.17215989 |
20588 | | Rotation angle (degrees) 166.44743618 |
20589 | | Shift along axis 46.83836222 |
20590 | | |
20591 | | |
20592 | | > fitmap #25.11 inMap #4 |
20593 | | |
20594 | | Fit molecule COPI_cyto_J3987_model K (#25.11) to map |
20595 | | W20_J3987_consensus_resampled.mrc (#4) using 319 atoms |
20596 | | average map value = 0.3247, steps = 352 |
20597 | | shifted from previous position = 17.9 |
20598 | | rotated from previous position = 29.5 degrees |
20599 | | atoms outside contour = 122, contour level = 0.27665 |
20600 | | |
20601 | | Position of COPI_cyto_J3987_model K (#25.11) relative to |
20602 | | W20_J3987_consensus_resampled.mrc (#4) coordinates: |
20603 | | Matrix rotation and translation |
20604 | | -0.11943694 -0.99009806 0.07376081 250.46546690 |
20605 | | -0.55633477 0.12827455 0.82099774 113.52890835 |
20606 | | -0.82232990 0.05702176 -0.56614669 259.81159734 |
20607 | | Axis -0.60879152 0.71407012 0.34565408 |
20608 | | Axis point 236.23455332 0.00000000 37.79456885 |
20609 | | Rotation angle (degrees) 141.13755065 |
20610 | | Shift along axis 18.39128867 |
20611 | | |
20612 | | |
20613 | | > select add #25.11 |
20614 | | |
20615 | | 319 atoms, 320 bonds, 37 residues, 1 model selected |
20616 | | |
20617 | | > view matrix models |
20618 | | > #25.11,-0.13737,-0.98867,0.060447,253.17,-0.58792,0.1305,0.79832,118.22,-0.79717,0.074131,-0.59919,261.16 |
20619 | | |
20620 | | > ui tool show Matchmaker |
20621 | | |
20622 | | > matchmaker #25.11 to #26 |
20623 | | |
20624 | | Parameters |
20625 | | --- |
20626 | | Chain pairing | bb |
20627 | | Alignment algorithm | Needleman-Wunsch |
20628 | | Similarity matrix | BLOSUM-62 |
20629 | | SS fraction | 0.3 |
20630 | | Gap open (HH/SS/other) | 18/18/6 |
20631 | | Gap extend | 1 |
20632 | | SS matrix | | | H | S | O |
20633 | | ---|---|---|--- |
20634 | | H | 6 | -9 | -6 |
20635 | | S | | 6 | -6 |
20636 | | O | | | 4 |
20637 | | Iteration cutoff | 2 |
20638 | | |
20639 | | Matchmaker COPI_cyto_J4021_model, chain K (#26) with COPI_cyto_J3987_model K, |
20640 | | chain K (#25.11), sequence alignment score = 192.1 |
20641 | | RMSD between 37 pruned atom pairs is 0.000 angstroms; (across all 37 pairs: |
20642 | | 0.000) |
20643 | | |
20644 | | |
20645 | | > select subtract #25.11 |
20646 | | |
20647 | | Nothing selected |
20648 | | |
20649 | | > hide #!25 models |
20650 | | |
20651 | | > show #!26 models |
20652 | | |
20653 | | > split #26 chains |
20654 | | |
20655 | | Split COPI_cyto_J4021_model (#26) into 13 models |
20656 | | Chain information for COPI_cyto_J4021_model A #26.1 |
20657 | | --- |
20658 | | Chain | Description |
20659 | | A | No description available |
20660 | | |
20661 | | Chain information for COPI_cyto_J4021_model B #26.2 |
20662 | | --- |
20663 | | Chain | Description |
20664 | | B | No description available |
20665 | | |
20666 | | Chain information for COPI_cyto_J4021_model C #26.3 |
20667 | | --- |
20668 | | Chain | Description |
20669 | | C | No description available |
20670 | | |
20671 | | Chain information for COPI_cyto_J4021_model D #26.4 |
20672 | | --- |
20673 | | Chain | Description |
20674 | | D | No description available |
20675 | | |
20676 | | Chain information for COPI_cyto_J4021_model E #26.5 |
20677 | | --- |
20678 | | Chain | Description |
20679 | | E | No description available |
20680 | | |
20681 | | Chain information for COPI_cyto_J4021_model F #26.6 |
20682 | | --- |
20683 | | Chain | Description |
20684 | | F | No description available |
20685 | | |
20686 | | Chain information for COPI_cyto_J4021_model G #26.7 |
20687 | | --- |
20688 | | Chain | Description |
20689 | | G | No description available |
20690 | | |
20691 | | Chain information for COPI_cyto_J4021_model H #26.8 |
20692 | | --- |
20693 | | Chain | Description |
20694 | | H | No description available |
20695 | | |
20696 | | Chain information for COPI_cyto_J4021_model I #26.9 |
20697 | | --- |
20698 | | Chain | Description |
20699 | | I | No description available |
20700 | | |
20701 | | Chain information for COPI_cyto_J4021_model J #26.10 |
20702 | | --- |
20703 | | Chain | Description |
20704 | | J | No description available |
20705 | | |
20706 | | Chain information for COPI_cyto_J4021_model K #26.11 |
20707 | | --- |
20708 | | Chain | Description |
20709 | | K | No description available |
20710 | | |
20711 | | Chain information for COPI_cyto_J4021_model L #26.12 |
20712 | | --- |
20713 | | Chain | Description |
20714 | | L | No description available |
20715 | | |
20716 | | Chain information for COPI_cyto_J4021_model M #26.13 |
20717 | | --- |
20718 | | Chain | Description |
20719 | | M | No description available |
20720 | | |
20721 | | |
20722 | | > hide #!4 models |
20723 | | |
20724 | | > show #!6 models |
20725 | | |
20726 | | > fitmap #26.1 inMap #6 |
20727 | | |
20728 | | Fit molecule COPI_cyto_J4021_model A (#26.1) to map |
20729 | | W20_J4021_consensus_resampled.mrc (#6) using 2590 atoms |
20730 | | average map value = 0.2703, steps = 84 |
20731 | | shifted from previous position = 0.378 |
20732 | | rotated from previous position = 1.1 degrees |
20733 | | atoms outside contour = 937, contour level = 0.25941 |
20734 | | |
20735 | | Position of COPI_cyto_J4021_model A (#26.1) relative to |
20736 | | W20_J4021_consensus_resampled.mrc (#6) coordinates: |
20737 | | Matrix rotation and translation |
20738 | | -0.37414892 -0.91220652 -0.16700861 276.47462167 |
20739 | | -0.79157778 0.22032122 0.56996772 162.80224157 |
20740 | | -0.48313272 0.34545310 -0.80451534 242.25699208 |
20741 | | Axis -0.55289681 0.77849727 0.29706416 |
20742 | | Axis point 211.11351512 0.00000000 70.18074399 |
20743 | | Rotation angle (degrees) 168.28551633 |
20744 | | Shift along axis 45.84503360 |
20745 | | |
20746 | | |
20747 | | > fitmap #26.2 inMap #6 |
20748 | | |
20749 | | Fit molecule COPI_cyto_J4021_model B (#26.2) to map |
20750 | | W20_J4021_consensus_resampled.mrc (#6) using 2426 atoms |
20751 | | average map value = 0.2468, steps = 64 |
20752 | | shifted from previous position = 0.762 |
20753 | | rotated from previous position = 1.85 degrees |
20754 | | atoms outside contour = 1379, contour level = 0.25941 |
20755 | | |
20756 | | Position of COPI_cyto_J4021_model B (#26.2) relative to |
20757 | | W20_J4021_consensus_resampled.mrc (#6) coordinates: |
20758 | | Matrix rotation and translation |
20759 | | -0.37049992 -0.90946831 -0.18867225 277.51418546 |
20760 | | -0.80121018 0.21016989 0.56025966 165.92440083 |
20761 | | -0.46988517 0.35874228 -0.80654319 240.14558920 |
20762 | | Axis -0.55590253 0.77574936 0.29863910 |
20763 | | Axis point 211.54042372 0.00000000 70.27796042 |
20764 | | Rotation angle (degrees) 169.55728062 |
20765 | | Shift along axis 46.16177391 |
20766 | | |
20767 | | |
20768 | | > fitmap #26.3 inMap #6 |
20769 | | |
20770 | | Fit molecule COPI_cyto_J4021_model C (#26.3) to map |
20771 | | W20_J4021_consensus_resampled.mrc (#6) using 1612 atoms |
20772 | | average map value = 0.268, steps = 88 |
20773 | | shifted from previous position = 1.42 |
20774 | | rotated from previous position = 8.83 degrees |
20775 | | atoms outside contour = 658, contour level = 0.25941 |
20776 | | |
20777 | | Position of COPI_cyto_J4021_model C (#26.3) relative to |
20778 | | W20_J4021_consensus_resampled.mrc (#6) coordinates: |
20779 | | Matrix rotation and translation |
20780 | | -0.45746641 -0.88778349 -0.05064549 275.77079696 |
20781 | | -0.69608083 0.32207858 0.64166727 143.83899735 |
20782 | | -0.55334978 0.32879458 -0.76530919 246.76254892 |
20783 | | Axis -0.50270755 0.80771908 0.30801786 |
20784 | | Axis point 205.38970331 0.00000000 66.05855727 |
20785 | | Rotation angle (degrees) 161.86918570 |
20786 | | Shift along axis 53.55671210 |
20787 | | |
20788 | | |
20789 | | > fitmap #26.4 inMap #6 |
20790 | | |
20791 | | Fit molecule COPI_cyto_J4021_model D (#26.4) to map |
20792 | | W20_J4021_consensus_resampled.mrc (#6) using 4745 atoms |
20793 | | average map value = 0.3102, steps = 84 |
20794 | | shifted from previous position = 1.18 |
20795 | | rotated from previous position = 1.93 degrees |
20796 | | atoms outside contour = 1692, contour level = 0.25941 |
20797 | | |
20798 | | Position of COPI_cyto_J4021_model D (#26.4) relative to |
20799 | | W20_J4021_consensus_resampled.mrc (#6) coordinates: |
20800 | | Matrix rotation and translation |
20801 | | -0.36883139 -0.91668768 -0.15377619 275.04474343 |
20802 | | -0.80433110 0.23185065 0.54708023 165.03207524 |
20803 | | -0.46584859 0.32546733 -0.82283420 243.51890015 |
20804 | | Axis -0.55555262 0.78232196 0.28166228 |
20805 | | Axis point 210.98432459 0.00000000 72.73645940 |
20806 | | Rotation angle (degrees) 168.49504583 |
20807 | | Shift along axis 44.89647919 |
20808 | | |
20809 | | |
20810 | | > fitmap #26.5 inMap #6 |
20811 | | |
20812 | | Fit molecule COPI_cyto_J4021_model E (#26.5) to map |
20813 | | W20_J4021_consensus_resampled.mrc (#6) using 1876 atoms |
20814 | | average map value = 0.2655, steps = 44 |
20815 | | shifted from previous position = 0.37 |
20816 | | rotated from previous position = 1.44 degrees |
20817 | | atoms outside contour = 812, contour level = 0.25941 |
20818 | | |
20819 | | Position of COPI_cyto_J4021_model E (#26.5) relative to |
20820 | | W20_J4021_consensus_resampled.mrc (#6) coordinates: |
20821 | | Matrix rotation and translation |
20822 | | -0.37950572 -0.91098051 -0.16152380 276.55156047 |
20823 | | -0.78154710 0.22222838 0.58292255 160.94019650 |
20824 | | -0.49513590 0.34746090 -0.79631111 243.08456807 |
20825 | | Axis -0.54968179 0.77881257 0.30216041 |
20826 | | Axis point 210.99674193 0.00000000 69.29658337 |
20827 | | Rotation angle (degrees) 167.63257816 |
20828 | | Shift along axis 46.77742440 |
20829 | | |
20830 | | |
20831 | | > fitmap #26.6 inMap #6 |
20832 | | |
20833 | | Fit molecule COPI_cyto_J4021_model F (#26.6) to map |
20834 | | W20_J4021_consensus_resampled.mrc (#6) using 2400 atoms |
20835 | | average map value = 0.3003, steps = 72 |
20836 | | shifted from previous position = 3.77 |
20837 | | rotated from previous position = 7.24 degrees |
20838 | | atoms outside contour = 749, contour level = 0.25941 |
20839 | | |
20840 | | Position of COPI_cyto_J4021_model F (#26.6) relative to |
20841 | | W20_J4021_consensus_resampled.mrc (#6) coordinates: |
20842 | | Matrix rotation and translation |
20843 | | -0.50121599 -0.84817893 -0.17139150 277.75765218 |
20844 | | -0.73837669 0.31593590 0.59580566 148.29415474 |
20845 | | -0.45120108 0.42517881 -0.78462767 230.60293751 |
20846 | | Axis -0.49366217 0.80955254 0.31768278 |
20847 | | Axis point 195.53368048 0.00000000 69.77740358 |
20848 | | Rotation angle (degrees) 170.04832921 |
20849 | | Shift along axis 56.19204834 |
20850 | | |
20851 | | |
20852 | | > fitmap #26.7 inMap #6 |
20853 | | |
20854 | | Fit molecule COPI_cyto_J4021_model G (#26.7) to map |
20855 | | W20_J4021_consensus_resampled.mrc (#6) using 2302 atoms |
20856 | | average map value = 0.3136, steps = 76 |
20857 | | shifted from previous position = 3.03 |
20858 | | rotated from previous position = 3.18 degrees |
20859 | | atoms outside contour = 723, contour level = 0.25941 |
20860 | | |
20861 | | Position of COPI_cyto_J4021_model G (#26.7) relative to |
20862 | | W20_J4021_consensus_resampled.mrc (#6) coordinates: |
20863 | | Matrix rotation and translation |
20864 | | -0.43399059 -0.88593113 -0.16364048 278.30290158 |
20865 | | -0.75953045 0.26210802 0.59532587 156.65606797 |
20866 | | -0.48452624 0.38265575 -0.78664408 237.63722363 |
20867 | | Axis -0.52487405 0.79195238 0.31195938 |
20868 | | Axis point 205.37019802 0.00000000 68.21146617 |
20869 | | Rotation angle (degrees) 168.31145195 |
20870 | | Shift along axis 52.12333508 |
20871 | | |
20872 | | |
20873 | | > fitmap #26.8 inMap #6 |
20874 | | |
20875 | | Fit molecule COPI_cyto_J4021_model H (#26.8) to map |
20876 | | W20_J4021_consensus_resampled.mrc (#6) using 420 atoms |
20877 | | average map value = 0.4011, steps = 52 |
20878 | | shifted from previous position = 2.32 |
20879 | | rotated from previous position = 1.55 degrees |
20880 | | atoms outside contour = 112, contour level = 0.25941 |
20881 | | |
20882 | | Position of COPI_cyto_J4021_model H (#26.8) relative to |
20883 | | W20_J4021_consensus_resampled.mrc (#6) coordinates: |
20884 | | Matrix rotation and translation |
20885 | | -0.41519620 -0.89510830 -0.16246005 278.00922432 |
20886 | | -0.77921085 0.25774528 0.57131239 160.68655904 |
20887 | | -0.46951315 0.36379736 -0.80449295 240.32587092 |
20888 | | Axis -0.53442036 0.79076406 0.29847457 |
20889 | | Axis point 207.26688590 0.00000000 70.94580099 |
20890 | | Rotation angle (degrees) 168.80494855 |
20891 | | Shift along axis 50.22252640 |
20892 | | |
20893 | | |
20894 | | > fitmap #26.9 inMap #6 |
20895 | | |
20896 | | Fit molecule COPI_cyto_J4021_model I (#26.9) to map |
20897 | | W20_J4021_consensus_resampled.mrc (#6) using 1573 atoms |
20898 | | average map value = 0.2888, steps = 112 |
20899 | | shifted from previous position = 1.44 |
20900 | | rotated from previous position = 3.06 degrees |
20901 | | atoms outside contour = 702, contour level = 0.25941 |
20902 | | |
20903 | | Position of COPI_cyto_J4021_model I (#26.9) relative to |
20904 | | W20_J4021_consensus_resampled.mrc (#6) coordinates: |
20905 | | Matrix rotation and translation |
20906 | | -0.37846625 -0.91010040 -0.16876189 276.33040233 |
20907 | | -0.76836367 0.20723976 0.60553197 162.64450565 |
20908 | | -0.51612071 0.35884392 -0.77772133 243.67694950 |
20909 | | Axis -0.54941463 0.77362488 0.31567087 |
20910 | | Axis point 212.64433272 0.00000000 66.29298345 |
20911 | | Rotation angle (degrees) 167.02647454 |
20912 | | Shift along axis 50.92758584 |
20913 | | |
20914 | | |
20915 | | > fitmap #26.10 inMap #6 |
20916 | | |
20917 | | Fit molecule COPI_cyto_J4021_model J (#26.10) to map |
20918 | | W20_J4021_consensus_resampled.mrc (#6) using 1112 atoms |
20919 | | average map value = 0.3374, steps = 68 |
20920 | | shifted from previous position = 0.981 |
20921 | | rotated from previous position = 4.81 degrees |
20922 | | atoms outside contour = 408, contour level = 0.25941 |
20923 | | |
20924 | | Position of COPI_cyto_J4021_model J (#26.10) relative to |
20925 | | W20_J4021_consensus_resampled.mrc (#6) coordinates: |
20926 | | Matrix rotation and translation |
20927 | | -0.36533925 -0.92641223 -0.09103639 268.10331410 |
20928 | | -0.79596275 0.26018434 0.54657793 163.12428402 |
20929 | | -0.48267023 0.27214795 -0.83244517 250.25943922 |
20930 | | Axis -0.55363524 0.79008238 0.26316888 |
20931 | | Axis point 210.17105433 0.00000000 74.56081239 |
20932 | | Rotation angle (degrees) 165.65005163 |
20933 | | Shift along axis 46.31067629 |
20934 | | |
20935 | | |
20936 | | > hide #!26 models |
20937 | | |
20938 | | > split #27 chains |
20939 | | |
20940 | | Split COPI_cyto_J4022_model (#27) into 13 models |
20941 | | Chain information for COPI_cyto_J4022_model A #27.1 |
20942 | | --- |
20943 | | Chain | Description |
20944 | | A | No description available |
20945 | | |
20946 | | Chain information for COPI_cyto_J4022_model B #27.2 |
20947 | | --- |
20948 | | Chain | Description |
20949 | | B | No description available |
20950 | | |
20951 | | Chain information for COPI_cyto_J4022_model C #27.3 |
20952 | | --- |
20953 | | Chain | Description |
20954 | | C | No description available |
20955 | | |
20956 | | Chain information for COPI_cyto_J4022_model D #27.4 |
20957 | | --- |
20958 | | Chain | Description |
20959 | | D | No description available |
20960 | | |
20961 | | Chain information for COPI_cyto_J4022_model E #27.5 |
20962 | | --- |
20963 | | Chain | Description |
20964 | | E | No description available |
20965 | | |
20966 | | Chain information for COPI_cyto_J4022_model F #27.6 |
20967 | | --- |
20968 | | Chain | Description |
20969 | | F | No description available |
20970 | | |
20971 | | Chain information for COPI_cyto_J4022_model G #27.7 |
20972 | | --- |
20973 | | Chain | Description |
20974 | | G | No description available |
20975 | | |
20976 | | Chain information for COPI_cyto_J4022_model H #27.8 |
20977 | | --- |
20978 | | Chain | Description |
20979 | | H | No description available |
20980 | | |
20981 | | Chain information for COPI_cyto_J4022_model I #27.9 |
20982 | | --- |
20983 | | Chain | Description |
20984 | | I | No description available |
20985 | | |
20986 | | Chain information for COPI_cyto_J4022_model J #27.10 |
20987 | | --- |
20988 | | Chain | Description |
20989 | | J | No description available |
20990 | | |
20991 | | Chain information for COPI_cyto_J4022_model K #27.11 |
20992 | | --- |
20993 | | Chain | Description |
20994 | | K | No description available |
20995 | | |
20996 | | Chain information for COPI_cyto_J4022_model L #27.12 |
20997 | | --- |
20998 | | Chain | Description |
20999 | | L | No description available |
21000 | | |
21001 | | Chain information for COPI_cyto_J4022_model M #27.13 |
21002 | | --- |
21003 | | Chain | Description |
21004 | | M | No description available |
21005 | | |
21006 | | |
21007 | | > hide #!6 models |
21008 | | |
21009 | | > show #!8 models |
21010 | | |
21011 | | > color #8 darkgrey models |
21012 | | |
21013 | | > color #8 #a9a9a9c0 models |
21014 | | |
21015 | | > color #8 #a9a9a9bf models |
21016 | | |
21017 | | > hide #!27 models |
21018 | | |
21019 | | > show #!27 models |
21020 | | |
21021 | | > show #27.1 models |
21022 | | |
21023 | | > show #27.2 models |
21024 | | |
21025 | | > show #27.3 models |
21026 | | |
21027 | | > show #!27.4 models |
21028 | | |
21029 | | > show #27.5 models |
21030 | | |
21031 | | > show #27.6 models |
21032 | | |
21033 | | > show #27.7 models |
21034 | | |
21035 | | > show #27.8 models |
21036 | | |
21037 | | > show #27.9 models |
21038 | | |
21039 | | > show #27.10 models |
21040 | | |
21041 | | > show #27.11 models |
21042 | | |
21043 | | > show #27.12 models |
21044 | | |
21045 | | > show #27.13 models |
21046 | | |
21047 | | > fitmap #27.1 inMap #8 |
21048 | | |
21049 | | Fit molecule COPI_cyto_J4022_model A (#27.1) to map |
21050 | | W20_J4022_consensus_resampled.mrc (#8) using 2590 atoms |
21051 | | average map value = 0.2646, steps = 56 |
21052 | | shifted from previous position = 1.25 |
21053 | | rotated from previous position = 2.42 degrees |
21054 | | atoms outside contour = 957, contour level = 0.25565 |
21055 | | |
21056 | | Position of COPI_cyto_J4022_model A (#27.1) relative to |
21057 | | W20_J4022_consensus_resampled.mrc (#8) coordinates: |
21058 | | Matrix rotation and translation |
21059 | | -0.39256776 -0.89898632 -0.19420133 280.18889645 |
21060 | | -0.78978108 0.22129853 0.57207763 162.30513777 |
21061 | | -0.47131349 0.37795577 -0.79687705 238.21825154 |
21062 | | Axis -0.54600989 0.77943814 0.30716346 |
21063 | | Axis point 209.81036508 0.00000000 69.55927246 |
21064 | | Rotation angle (degrees) 169.76045090 |
21065 | | Shift along axis 46.69284574 |
21066 | | |
21067 | | |
21068 | | > fitmap #27.2 inMap #8 |
21069 | | |
21070 | | Fit molecule COPI_cyto_J4022_model B (#27.2) to map |
21071 | | W20_J4022_consensus_resampled.mrc (#8) using 2426 atoms |
21072 | | average map value = 0.2446, steps = 52 |
21073 | | shifted from previous position = 1.11 |
21074 | | rotated from previous position = 2.79 degrees |
21075 | | atoms outside contour = 1336, contour level = 0.25565 |
21076 | | |
21077 | | Position of COPI_cyto_J4022_model B (#27.2) relative to |
21078 | | W20_J4022_consensus_resampled.mrc (#8) coordinates: |
21079 | | Matrix rotation and translation |
21080 | | -0.38976486 -0.89769297 -0.20550111 280.56757169 |
21081 | | -0.80102851 0.22038063 0.55658397 165.91242827 |
21082 | | -0.45435305 0.38154912 -0.80497428 236.80345949 |
21083 | | Axis -0.54830136 0.77953535 0.30280399 |
21084 | | Axis point 210.09627043 0.00000000 70.38992448 |
21085 | | Rotation angle (degrees) 170.81541733 |
21086 | | Shift along axis 47.20405452 |
21087 | | |
21088 | | |
21089 | | > fitmap #27.3 inMap #8 |
21090 | | |
21091 | | Fit molecule COPI_cyto_J4022_model C (#27.3) to map |
21092 | | W20_J4022_consensus_resampled.mrc (#8) using 1612 atoms |
21093 | | average map value = 0.2655, steps = 76 |
21094 | | shifted from previous position = 1.78 |
21095 | | rotated from previous position = 8.48 degrees |
21096 | | atoms outside contour = 633, contour level = 0.25565 |
21097 | | |
21098 | | Position of COPI_cyto_J4022_model C (#27.3) relative to |
21099 | | W20_J4022_consensus_resampled.mrc (#8) coordinates: |
21100 | | Matrix rotation and translation |
21101 | | -0.44384488 -0.89370270 -0.06555325 276.26301732 |
21102 | | -0.70850239 0.30519488 0.63630219 146.54842255 |
21103 | | -0.54865846 0.32886410 -0.76864967 245.76532786 |
21104 | | Axis -0.51083267 0.80271747 0.30772494 |
21105 | | Axis point 206.80155193 0.00000000 65.73198008 |
21106 | | Rotation angle (degrees) 162.48721631 |
21107 | | Shift along axis 52.14092594 |
21108 | | |
21109 | | |
21110 | | > fitmap #27.4 inMap #8 |
21111 | | |
21112 | | Fit molecule COPI_cyto_J4022_model D (#27.4) to map |
21113 | | W20_J4022_consensus_resampled.mrc (#8) using 4745 atoms |
21114 | | average map value = 0.3044, steps = 100 |
21115 | | shifted from previous position = 0.615 |
21116 | | rotated from previous position = 1.41 degrees |
21117 | | atoms outside contour = 1677, contour level = 0.25565 |
21118 | | |
21119 | | Position of COPI_cyto_J4022_model D (#27.4) relative to |
21120 | | W20_J4022_consensus_resampled.mrc (#8) coordinates: |
21121 | | Matrix rotation and translation |
21122 | | -0.37672030 -0.91435784 -0.14843034 275.26026826 |
21123 | | -0.79965882 0.24012360 0.55035120 163.64023290 |
21124 | | -0.46757631 0.32602210 -0.82163374 244.18290661 |
21125 | | Axis -0.55170270 0.78489010 0.28208449 |
21126 | | Axis point 210.28195260 0.00000000 73.03172771 |
21127 | | Rotation angle (degrees) 168.26963934 |
21128 | | Shift along axis 45.45797518 |
21129 | | |
21130 | | |
21131 | | > fitmap #27.5 inMap #8 |
21132 | | |
21133 | | Fit molecule COPI_cyto_J4022_model E (#27.5) to map |
21134 | | W20_J4022_consensus_resampled.mrc (#8) using 1876 atoms |
21135 | | average map value = 0.2625, steps = 52 |
21136 | | shifted from previous position = 0.552 |
21137 | | rotated from previous position = 1.61 degrees |
21138 | | atoms outside contour = 794, contour level = 0.25565 |
21139 | | |
21140 | | Position of COPI_cyto_J4022_model E (#27.5) relative to |
21141 | | W20_J4022_consensus_resampled.mrc (#8) coordinates: |
21142 | | Matrix rotation and translation |
21143 | | -0.39012142 -0.90485684 -0.17040949 278.02588034 |
21144 | | -0.78445694 0.22972243 0.57606852 161.46943730 |
21145 | | -0.48211265 0.35841557 -0.79944086 241.20120653 |
21146 | | Axis -0.54579308 0.78163622 0.30191841 |
21147 | | Axis point 210.00611855 0.00000000 69.84237754 |
21148 | | Rotation angle (degrees) 168.49862507 |
21149 | | Shift along axis 47.28884646 |
21150 | | |
21151 | | |
21152 | | > fitmap #27.6 inMap #8 |
21153 | | |
21154 | | Fit molecule COPI_cyto_J4022_model F (#27.6) to map |
21155 | | W20_J4022_consensus_resampled.mrc (#8) using 2400 atoms |
21156 | | average map value = 0.2992, steps = 68 |
21157 | | shifted from previous position = 0.833 |
21158 | | rotated from previous position = 1.23 degrees |
21159 | | atoms outside contour = 836, contour level = 0.25565 |
21160 | | |
21161 | | Position of COPI_cyto_J4022_model F (#27.6) relative to |
21162 | | W20_J4022_consensus_resampled.mrc (#8) coordinates: |
21163 | | Matrix rotation and translation |
21164 | | -0.37627915 -0.91501112 -0.14549456 274.06344417 |
21165 | | -0.78910526 0.23420927 0.56785467 161.94404988 |
21166 | | -0.48551716 0.32848239 -0.81016814 243.51929054 |
21167 | | Axis -0.55094864 0.78260937 0.28978988 |
21168 | | Axis point 210.19577001 0.00000000 70.73622595 |
21169 | | Rotation angle (degrees) 167.45323080 |
21170 | | Shift along axis 46.31347447 |
21171 | | |
21172 | | |
21173 | | > fitmap #27.7 inMap #8 |
21174 | | |
21175 | | Fit molecule COPI_cyto_J4022_model G (#27.7) to map |
21176 | | W20_J4022_consensus_resampled.mrc (#8) using 2302 atoms |
21177 | | average map value = 0.2826, steps = 96 |
21178 | | shifted from previous position = 2.92 |
21179 | | rotated from previous position = 2.79 degrees |
21180 | | atoms outside contour = 976, contour level = 0.25565 |
21181 | | |
21182 | | Position of COPI_cyto_J4022_model G (#27.7) relative to |
21183 | | W20_J4022_consensus_resampled.mrc (#8) coordinates: |
21184 | | Matrix rotation and translation |
21185 | | -0.33451130 -0.92639130 -0.17292012 274.85479282 |
21186 | | -0.79442923 0.17849847 0.58053467 167.37506321 |
21187 | | -0.50693629 0.33156820 -0.79566208 241.26568780 |
21188 | | Axis -0.56972315 0.76434688 0.30197578 |
21189 | | Axis point 216.29332055 0.00000000 65.86543953 |
21190 | | Rotation angle (degrees) 167.37918527 |
21191 | | Shift along axis 44.19786245 |
21192 | | |
21193 | | |
21194 | | > fitmap #27.8 inMap #8 |
21195 | | |
21196 | | Fit molecule COPI_cyto_J4022_model H (#27.8) to map |
21197 | | W20_J4022_consensus_resampled.mrc (#8) using 420 atoms |
21198 | | average map value = 0.3956, steps = 60 |
21199 | | shifted from previous position = 3.43 |
21200 | | rotated from previous position = 4.58 degrees |
21201 | | atoms outside contour = 107, contour level = 0.25565 |
21202 | | |
21203 | | Position of COPI_cyto_J4022_model H (#27.8) relative to |
21204 | | W20_J4022_consensus_resampled.mrc (#8) coordinates: |
21205 | | Matrix rotation and translation |
21206 | | -0.29409810 -0.94159516 -0.16402708 273.35842395 |
21207 | | -0.82182141 0.16150890 0.54637392 173.87777684 |
21208 | | -0.48797119 0.29548850 -0.82132252 245.93686122 |
21209 | | Axis -0.58771482 0.75885938 0.28057750 |
21210 | | Axis point 220.70161074 0.00000000 69.69363620 |
21211 | | Rotation angle (degrees) 167.67589275 |
21212 | | Shift along axis 40.29633569 |
21213 | | |
21214 | | |
21215 | | > fitmap #27.9 inMap #8 |
21216 | | |
21217 | | Fit molecule COPI_cyto_J4022_model I (#27.9) to map |
21218 | | W20_J4022_consensus_resampled.mrc (#8) using 1573 atoms |
21219 | | average map value = 0.2808, steps = 104 |
21220 | | shifted from previous position = 1.41 |
21221 | | rotated from previous position = 3.01 degrees |
21222 | | atoms outside contour = 658, contour level = 0.25565 |
21223 | | |
21224 | | Position of COPI_cyto_J4022_model I (#27.9) relative to |
21225 | | W20_J4022_consensus_resampled.mrc (#8) coordinates: |
21226 | | Matrix rotation and translation |
21227 | | -0.32433234 -0.92767253 -0.18501954 273.90297834 |
21228 | | -0.82148476 0.17924210 0.54132715 171.93921965 |
21229 | | -0.46901103 0.32756063 -0.82020284 242.13807924 |
21230 | | Axis -0.57622748 0.76552540 0.28623898 |
21231 | | Axis point 215.70475315 0.00000000 70.66930942 |
21232 | | Rotation angle (degrees) 169.31041717 |
21233 | | Shift along axis 43.10277500 |
21234 | | |
21235 | | |
21236 | | > fitmap #27.10 inMap #8 |
21237 | | |
21238 | | Fit molecule COPI_cyto_J4022_model J (#27.10) to map |
21239 | | W20_J4022_consensus_resampled.mrc (#8) using 1112 atoms |
21240 | | average map value = 0.33, steps = 88 |
21241 | | shifted from previous position = 0.662 |
21242 | | rotated from previous position = 3.39 degrees |
21243 | | atoms outside contour = 391, contour level = 0.25565 |
21244 | | |
21245 | | Position of COPI_cyto_J4022_model J (#27.10) relative to |
21246 | | W20_J4022_consensus_resampled.mrc (#8) coordinates: |
21247 | | Matrix rotation and translation |
21248 | | -0.38171857 -0.91794952 -0.10797977 270.82825471 |
21249 | | -0.78627841 0.26109129 0.55999787 160.73450027 |
21250 | | -0.48585720 0.29866375 -0.82142727 248.07112012 |
21251 | | Axis -0.54679573 0.79064209 0.27549866 |
21252 | | Axis point 209.01509765 0.00000000 73.28198391 |
21253 | | Rotation angle (degrees) 166.17432003 |
21254 | | Shift along axis 47.33898908 |
21255 | | |
21256 | | |
21257 | | > hide #!27 models |
21258 | | |
21259 | | > show #!28 models |
21260 | | |
21261 | | > split #28 chains |
21262 | | |
21263 | | Split COPI_cyto_J4023_model (#28) into 13 models |
21264 | | Chain information for COPI_cyto_J4023_model A #28.1 |
21265 | | --- |
21266 | | Chain | Description |
21267 | | A | No description available |
21268 | | |
21269 | | Chain information for COPI_cyto_J4023_model B #28.2 |
21270 | | --- |
21271 | | Chain | Description |
21272 | | B | No description available |
21273 | | |
21274 | | Chain information for COPI_cyto_J4023_model C #28.3 |
21275 | | --- |
21276 | | Chain | Description |
21277 | | C | No description available |
21278 | | |
21279 | | Chain information for COPI_cyto_J4023_model D #28.4 |
21280 | | --- |
21281 | | Chain | Description |
21282 | | D | No description available |
21283 | | |
21284 | | Chain information for COPI_cyto_J4023_model E #28.5 |
21285 | | --- |
21286 | | Chain | Description |
21287 | | E | No description available |
21288 | | |
21289 | | Chain information for COPI_cyto_J4023_model F #28.6 |
21290 | | --- |
21291 | | Chain | Description |
21292 | | F | No description available |
21293 | | |
21294 | | Chain information for COPI_cyto_J4023_model G #28.7 |
21295 | | --- |
21296 | | Chain | Description |
21297 | | G | No description available |
21298 | | |
21299 | | Chain information for COPI_cyto_J4023_model H #28.8 |
21300 | | --- |
21301 | | Chain | Description |
21302 | | H | No description available |
21303 | | |
21304 | | Chain information for COPI_cyto_J4023_model I #28.9 |
21305 | | --- |
21306 | | Chain | Description |
21307 | | I | No description available |
21308 | | |
21309 | | Chain information for COPI_cyto_J4023_model J #28.10 |
21310 | | --- |
21311 | | Chain | Description |
21312 | | J | No description available |
21313 | | |
21314 | | Chain information for COPI_cyto_J4023_model K #28.11 |
21315 | | --- |
21316 | | Chain | Description |
21317 | | K | No description available |
21318 | | |
21319 | | Chain information for COPI_cyto_J4023_model L #28.12 |
21320 | | --- |
21321 | | Chain | Description |
21322 | | L | No description available |
21323 | | |
21324 | | Chain information for COPI_cyto_J4023_model M #28.13 |
21325 | | --- |
21326 | | Chain | Description |
21327 | | M | No description available |
21328 | | |
21329 | | |
21330 | | > hide #!8 models |
21331 | | |
21332 | | > show #!10 models |
21333 | | |
21334 | | > color #10 darkgrey models |
21335 | | |
21336 | | > color #10 #a9a9a9c3 models |
21337 | | |
21338 | | > color #10 #a9a9a9c1 models |
21339 | | |
21340 | | > color #10 #a9a9a9c0 models |
21341 | | |
21342 | | > color #10 #a9a9a9bf models |
21343 | | |
21344 | | > fitmap #28.1 inMap #10 |
21345 | | |
21346 | | Fit molecule COPI_cyto_J4023_model A (#28.1) to map |
21347 | | W20_J4023_consensus_resampled.mrc (#10) using 2590 atoms |
21348 | | average map value = 0.2661, steps = 80 |
21349 | | shifted from previous position = 0.302 |
21350 | | rotated from previous position = 1.64 degrees |
21351 | | atoms outside contour = 989, contour level = 0.259 |
21352 | | |
21353 | | Position of COPI_cyto_J4023_model A (#28.1) relative to |
21354 | | W20_J4023_consensus_resampled.mrc (#10) coordinates: |
21355 | | Matrix rotation and translation |
21356 | | -0.38904601 -0.90521909 -0.17094331 278.16947599 |
21357 | | -0.78384179 0.22779200 0.57767021 160.52070200 |
21358 | | -0.48397858 0.35873280 -0.79817011 240.89357120 |
21359 | | Axis -0.54622301 0.78098606 0.30282204 |
21360 | | Axis point 209.92132129 0.00000000 69.65657344 |
21361 | | Rotation angle (degrees) 168.43904812 |
21362 | | Shift along axis 46.36974623 |
21363 | | |
21364 | | |
21365 | | > fitmap #28.2 inMap #10 |
21366 | | |
21367 | | Fit molecule COPI_cyto_J4023_model B (#28.2) to map |
21368 | | W20_J4023_consensus_resampled.mrc (#10) using 2426 atoms |
21369 | | average map value = 0.2462, steps = 52 |
21370 | | shifted from previous position = 0.807 |
21371 | | rotated from previous position = 1.71 degrees |
21372 | | atoms outside contour = 1372, contour level = 0.259 |
21373 | | |
21374 | | Position of COPI_cyto_J4023_model B (#28.2) relative to |
21375 | | W20_J4023_consensus_resampled.mrc (#10) coordinates: |
21376 | | Matrix rotation and translation |
21377 | | -0.37936173 -0.90522783 -0.19143475 278.45387337 |
21378 | | -0.80078335 0.21757935 0.55803698 165.73257662 |
21379 | | -0.46349835 0.36499563 -0.80743265 238.99424212 |
21380 | | Axis -0.55224090 0.77830294 0.29878840 |
21381 | | Axis point 210.75764690 0.00000000 70.47418382 |
21382 | | Rotation angle (degrees) 169.93414364 |
21383 | | Shift along axis 46.62524128 |
21384 | | |
21385 | | |
21386 | | > fitmap #28.3 inMap #10 |
21387 | | |
21388 | | Fit molecule COPI_cyto_J4023_model C (#28.3) to map |
21389 | | W20_J4023_consensus_resampled.mrc (#10) using 1612 atoms |
21390 | | average map value = 0.2674, steps = 100 |
21391 | | shifted from previous position = 1.78 |
21392 | | rotated from previous position = 13.1 degrees |
21393 | | atoms outside contour = 645, contour level = 0.259 |
21394 | | |
21395 | | Position of COPI_cyto_J4023_model C (#28.3) relative to |
21396 | | W20_J4023_consensus_resampled.mrc (#10) coordinates: |
21397 | | Matrix rotation and translation |
21398 | | -0.48320445 -0.87548576 -0.00617812 274.93344286 |
21399 | | -0.65530471 0.35698291 0.66568683 136.56063023 |
21400 | | -0.58059385 0.32571139 -0.74620566 247.99422947 |
21401 | | Axis -0.48370086 0.81725132 0.31326310 |
21402 | | Axis point 203.45931463 0.00000000 64.26982521 |
21403 | | Rotation angle (degrees) 159.42510249 |
21404 | | Shift along axis 56.30625377 |
21405 | | |
21406 | | |
21407 | | > fitmap #28.4 inMap #10 |
21408 | | |
21409 | | Fit molecule COPI_cyto_J4023_model D (#28.4) to map |
21410 | | W20_J4023_consensus_resampled.mrc (#10) using 4745 atoms |
21411 | | average map value = 0.2995, steps = 72 |
21412 | | shifted from previous position = 0.314 |
21413 | | rotated from previous position = 1.26 degrees |
21414 | | atoms outside contour = 1741, contour level = 0.259 |
21415 | | |
21416 | | Position of COPI_cyto_J4023_model D (#28.4) relative to |
21417 | | W20_J4023_consensus_resampled.mrc (#10) coordinates: |
21418 | | Matrix rotation and translation |
21419 | | -0.37631952 -0.91385434 -0.15249221 275.60221900 |
21420 | | -0.80067150 0.23796533 0.54981604 164.18546643 |
21421 | | -0.46616391 0.32900267 -0.82124812 243.17403703 |
21422 | | Axis -0.55210445 0.78428015 0.28299352 |
21423 | | Axis point 210.42278812 0.00000000 72.60304045 |
21424 | | Rotation angle (degrees) 168.46454551 |
21425 | | Shift along axis 45.42286901 |
21426 | | |
21427 | | |
21428 | | > fitmap #28.5 inMap #10 |
21429 | | |
21430 | | Fit molecule COPI_cyto_J4023_model E (#28.5) to map |
21431 | | W20_J4023_consensus_resampled.mrc (#10) using 1876 atoms |
21432 | | average map value = 0.2617, steps = 64 |
21433 | | shifted from previous position = 0.422 |
21434 | | rotated from previous position = 1.48 degrees |
21435 | | atoms outside contour = 839, contour level = 0.259 |
21436 | | |
21437 | | Position of COPI_cyto_J4023_model E (#28.5) relative to |
21438 | | W20_J4023_consensus_resampled.mrc (#10) coordinates: |
21439 | | Matrix rotation and translation |
21440 | | -0.38829706 -0.90758205 -0.15975054 277.05830941 |
21441 | | -0.78302109 0.23353287 0.57648971 160.99119705 |
21442 | | -0.48590471 0.34893730 -0.80133600 242.27299446 |
21443 | | Axis -0.54602993 0.78263260 0.29889383 |
21444 | | Axis point 210.04637843 0.00000000 70.01691196 |
21445 | | Rotation angle (degrees) 167.97315842 |
21446 | | Shift along axis 47.12873327 |
21447 | | |
21448 | | |
21449 | | > fitmap #28.6 inMap #10 |
21450 | | |
21451 | | Fit molecule COPI_cyto_J4023_model F (#28.6) to map |
21452 | | W20_J4023_consensus_resampled.mrc (#10) using 2400 atoms |
21453 | | average map value = 0.3111, steps = 56 |
21454 | | shifted from previous position = 2.06 |
21455 | | rotated from previous position = 4.34 degrees |
21456 | | atoms outside contour = 741, contour level = 0.259 |
21457 | | |
21458 | | Position of COPI_cyto_J4023_model F (#28.6) relative to |
21459 | | W20_J4023_consensus_resampled.mrc (#10) coordinates: |
21460 | | Matrix rotation and translation |
21461 | | -0.35666068 -0.90337062 -0.23814849 281.82055176 |
21462 | | -0.82036020 0.18087540 0.54248801 172.36641935 |
21463 | | -0.44699252 0.38885168 -0.80560044 235.22166541 |
21464 | | Axis -0.56435649 0.76715244 0.30492440 |
21465 | | Axis point 214.18344574 0.00000000 69.66627251 |
21466 | | Rotation angle (degrees) 172.17681998 |
21467 | | Shift along axis 44.90888683 |
21468 | | |
21469 | | |
21470 | | > fitmap #28.7 inMap #10 |
21471 | | |
21472 | | Fit molecule COPI_cyto_J4023_model G (#28.7) to map |
21473 | | W20_J4023_consensus_resampled.mrc (#10) using 2302 atoms |
21474 | | average map value = 0.2755, steps = 64 |
21475 | | shifted from previous position = 1.24 |
21476 | | rotated from previous position = 2.83 degrees |
21477 | | atoms outside contour = 963, contour level = 0.259 |
21478 | | |
21479 | | Position of COPI_cyto_J4023_model G (#28.7) relative to |
21480 | | W20_J4023_consensus_resampled.mrc (#10) coordinates: |
21481 | | Matrix rotation and translation |
21482 | | -0.37482050 -0.90249978 -0.21214088 278.18486892 |
21483 | | -0.80142293 0.20037819 0.56353340 166.38017125 |
21484 | | -0.46608036 0.38123844 -0.79838986 235.86468916 |
21485 | | Axis -0.55487743 0.77295215 0.30766217 |
21486 | | Axis point 210.65854364 0.00000000 68.54942126 |
21487 | | Rotation angle (degrees) 170.54540398 |
21488 | | Shift along axis 46.81204878 |
21489 | | |
21490 | | |
21491 | | > fitmap #28.8 inMap #10 |
21492 | | |
21493 | | Fit molecule COPI_cyto_J4023_model H (#28.8) to map |
21494 | | W20_J4023_consensus_resampled.mrc (#10) using 420 atoms |
21495 | | average map value = 0.3956, steps = 56 |
21496 | | shifted from previous position = 0.904 |
21497 | | rotated from previous position = 5.89 degrees |
21498 | | atoms outside contour = 91, contour level = 0.259 |
21499 | | |
21500 | | Position of COPI_cyto_J4023_model H (#28.8) relative to |
21501 | | W20_J4023_consensus_resampled.mrc (#10) coordinates: |
21502 | | Matrix rotation and translation |
21503 | | -0.28058215 -0.93606717 -0.21225441 275.82091929 |
21504 | | -0.83301532 0.12762624 0.53832800 180.46362086 |
21505 | | -0.47682192 0.32785640 -0.81556793 244.72635677 |
21506 | | Axis -0.59550860 0.74856765 0.29157501 |
21507 | | Axis point 223.51865643 0.00000000 69.31481173 |
21508 | | Rotation angle (degrees) 169.82147556 |
21509 | | Shift along axis 42.19158968 |
21510 | | |
21511 | | |
21512 | | > fitmap #28.9 inMap #10 |
21513 | | |
21514 | | Fit molecule COPI_cyto_J4023_model I (#28.9) to map |
21515 | | W20_J4023_consensus_resampled.mrc (#10) using 1573 atoms |
21516 | | average map value = 0.285, steps = 68 |
21517 | | shifted from previous position = 1.1 |
21518 | | rotated from previous position = 2.83 degrees |
21519 | | atoms outside contour = 662, contour level = 0.259 |
21520 | | |
21521 | | Position of COPI_cyto_J4023_model I (#28.9) relative to |
21522 | | W20_J4023_consensus_resampled.mrc (#10) coordinates: |
21523 | | Matrix rotation and translation |
21524 | | -0.34800803 -0.91546944 -0.20200525 275.41256013 |
21525 | | -0.79922182 0.17708111 0.57435771 168.85645411 |
21526 | | -0.49003561 0.36132810 -0.79328880 241.32530949 |
21527 | | Axis -0.56560752 0.76473942 0.30864502 |
21528 | | Axis point 214.22374441 0.00000000 67.79866361 |
21529 | | Rotation angle (degrees) 169.14528401 |
21530 | | Shift along axis 47.83962632 |
21531 | | |
21532 | | |
21533 | | > fitmap #28.10 inMap #10 |
21534 | | |
21535 | | Fit molecule COPI_cyto_J4023_model J (#28.10) to map |
21536 | | W20_J4023_consensus_resampled.mrc (#10) using 1112 atoms |
21537 | | average map value = 0.3207, steps = 84 |
21538 | | shifted from previous position = 0.351 |
21539 | | rotated from previous position = 3.94 degrees |
21540 | | atoms outside contour = 429, contour level = 0.259 |
21541 | | |
21542 | | Position of COPI_cyto_J4023_model J (#28.10) relative to |
21543 | | W20_J4023_consensus_resampled.mrc (#10) coordinates: |
21544 | | Matrix rotation and translation |
21545 | | -0.37572939 -0.92009445 -0.11069619 271.04311764 |
21546 | | -0.80434258 0.26444766 0.53207186 165.17448016 |
21547 | | -0.46028301 0.28895269 -0.83943190 247.79248250 |
21548 | | Axis -0.55095733 0.79223463 0.26231720 |
21549 | | Axis point 209.56379921 0.00000000 75.63736830 |
21550 | | Rotation angle (degrees) 167.25376251 |
21551 | | Shift along axis 46.52397993 |
21552 | | |
21553 | | |
21554 | | > hide #!10 models |
21555 | | |
21556 | | > show #!12 models |
21557 | | |
21558 | | > color #12 darkgrey models |
21559 | | |
21560 | | > color #12 #a9a9a9c0 models |
21561 | | |
21562 | | > color #12 #a9a9a9bf models |
21563 | | |
21564 | | > hide #!28 models |
21565 | | |
21566 | | > show #!29 models |
21567 | | |
21568 | | > split #29 chains |
21569 | | |
21570 | | Split COPI_cyto_J4024_model (#29) into 13 models |
21571 | | Chain information for COPI_cyto_J4024_model A #29.1 |
21572 | | --- |
21573 | | Chain | Description |
21574 | | A | No description available |
21575 | | |
21576 | | Chain information for COPI_cyto_J4024_model B #29.2 |
21577 | | --- |
21578 | | Chain | Description |
21579 | | B | No description available |
21580 | | |
21581 | | Chain information for COPI_cyto_J4024_model C #29.3 |
21582 | | --- |
21583 | | Chain | Description |
21584 | | C | No description available |
21585 | | |
21586 | | Chain information for COPI_cyto_J4024_model D #29.4 |
21587 | | --- |
21588 | | Chain | Description |
21589 | | D | No description available |
21590 | | |
21591 | | Chain information for COPI_cyto_J4024_model E #29.5 |
21592 | | --- |
21593 | | Chain | Description |
21594 | | E | No description available |
21595 | | |
21596 | | Chain information for COPI_cyto_J4024_model F #29.6 |
21597 | | --- |
21598 | | Chain | Description |
21599 | | F | No description available |
21600 | | |
21601 | | Chain information for COPI_cyto_J4024_model G #29.7 |
21602 | | --- |
21603 | | Chain | Description |
21604 | | G | No description available |
21605 | | |
21606 | | Chain information for COPI_cyto_J4024_model H #29.8 |
21607 | | --- |
21608 | | Chain | Description |
21609 | | H | No description available |
21610 | | |
21611 | | Chain information for COPI_cyto_J4024_model I #29.9 |
21612 | | --- |
21613 | | Chain | Description |
21614 | | I | No description available |
21615 | | |
21616 | | Chain information for COPI_cyto_J4024_model J #29.10 |
21617 | | --- |
21618 | | Chain | Description |
21619 | | J | No description available |
21620 | | |
21621 | | Chain information for COPI_cyto_J4024_model K #29.11 |
21622 | | --- |
21623 | | Chain | Description |
21624 | | K | No description available |
21625 | | |
21626 | | Chain information for COPI_cyto_J4024_model L #29.12 |
21627 | | --- |
21628 | | Chain | Description |
21629 | | L | No description available |
21630 | | |
21631 | | Chain information for COPI_cyto_J4024_model M #29.13 |
21632 | | --- |
21633 | | Chain | Description |
21634 | | M | No description available |
21635 | | |
21636 | | |
21637 | | > fitmap #29.1 inMap #12 |
21638 | | |
21639 | | Fit molecule COPI_cyto_J4024_model A (#29.1) to map |
21640 | | W20_J4024_consensus_resampled.mrc (#12) using 2590 atoms |
21641 | | average map value = 0.2692, steps = 100 |
21642 | | shifted from previous position = 0.874 |
21643 | | rotated from previous position = 1.25 degrees |
21644 | | atoms outside contour = 892, contour level = 0.25569 |
21645 | | |
21646 | | Position of COPI_cyto_J4024_model A (#29.1) relative to |
21647 | | W20_J4024_consensus_resampled.mrc (#12) coordinates: |
21648 | | Matrix rotation and translation |
21649 | | -0.38195228 -0.90644822 -0.18017798 277.95847443 |
21650 | | -0.79414648 0.22219499 0.56565075 163.75119780 |
21651 | | -0.47269847 0.35913930 -0.80472054 240.33850306 |
21652 | | Axis -0.55030232 0.77949527 0.29925655 |
21653 | | Axis point 210.52708604 0.00000000 70.30220517 |
21654 | | Rotation angle (degrees) 169.18522876 |
21655 | | Shift along axis 46.60496312 |
21656 | | |
21657 | | |
21658 | | > fitmap #29.2 inMap #12 |
21659 | | |
21660 | | Fit molecule COPI_cyto_J4024_model B (#29.2) to map |
21661 | | W20_J4024_consensus_resampled.mrc (#12) using 2426 atoms |
21662 | | average map value = 0.2485, steps = 64 |
21663 | | shifted from previous position = 0.888 |
21664 | | rotated from previous position = 2.24 degrees |
21665 | | atoms outside contour = 1271, contour level = 0.25569 |
21666 | | |
21667 | | Position of COPI_cyto_J4024_model B (#29.2) relative to |
21668 | | W20_J4024_consensus_resampled.mrc (#12) coordinates: |
21669 | | Matrix rotation and translation |
21670 | | -0.37729450 -0.90386908 -0.20166693 279.03898781 |
21671 | | -0.80059309 0.20887819 0.56162321 165.93012621 |
21672 | | -0.46551002 0.37335050 -0.80243993 238.54192108 |
21673 | | Axis -0.55344239 0.77558746 0.30358789 |
21674 | | Axis point 211.11340356 0.00000000 69.95800816 |
21675 | | Rotation angle (degrees) 170.20680766 |
21676 | | Shift along axis 46.67975878 |
21677 | | |
21678 | | |
21679 | | > fitmap #29.3 inMap #12 |
21680 | | |
21681 | | Fit molecule COPI_cyto_J4024_model C (#29.3) to map |
21682 | | W20_J4024_consensus_resampled.mrc (#12) using 1612 atoms |
21683 | | average map value = 0.2695, steps = 160 |
21684 | | shifted from previous position = 2.41 |
21685 | | rotated from previous position = 18.3 degrees |
21686 | | atoms outside contour = 621, contour level = 0.25569 |
21687 | | |
21688 | | Position of COPI_cyto_J4024_model C (#29.3) relative to |
21689 | | W20_J4024_consensus_resampled.mrc (#12) coordinates: |
21690 | | Matrix rotation and translation |
21691 | | -0.48164802 -0.86999176 0.10549661 268.61459966 |
21692 | | -0.62073770 0.42365137 0.65970011 129.62131912 |
21693 | | -0.61862744 0.25225752 -0.74408753 255.46187775 |
21694 | | Axis -0.46969429 0.83476038 0.28733670 |
21695 | | Axis point 203.50844953 0.00000000 65.15506927 |
21696 | | Rotation angle (degrees) 154.29538375 |
21697 | | Shift along axis 55.43957254 |
21698 | | |
21699 | | |
21700 | | > fitmap #29.4 inMap #12 |
21701 | | |
21702 | | Fit molecule COPI_cyto_J4024_model D (#29.4) to map |
21703 | | W20_J4024_consensus_resampled.mrc (#12) using 4745 atoms |
21704 | | average map value = 0.311, steps = 96 |
21705 | | shifted from previous position = 0.393 |
21706 | | rotated from previous position = 1.84 degrees |
21707 | | atoms outside contour = 1582, contour level = 0.25569 |
21708 | | |
21709 | | Position of COPI_cyto_J4024_model D (#29.4) relative to |
21710 | | W20_J4024_consensus_resampled.mrc (#12) coordinates: |
21711 | | Matrix rotation and translation |
21712 | | -0.36490275 -0.91923092 -0.14785303 274.26364105 |
21713 | | -0.80817834 0.23388135 0.54050653 165.94975145 |
21714 | | -0.46227025 0.31672393 -0.82824645 244.92475483 |
21715 | | Axis -0.55725013 0.78294306 0.27653654 |
21716 | | Axis point 211.24999279 0.00000000 73.77234724 |
21717 | | Rotation angle (degrees) 168.41673025 |
21718 | | Shift along axis 44.82640132 |
21719 | | |
21720 | | |
21721 | | > fitmap #29.5 inMap #12 |
21722 | | |
21723 | | Fit molecule COPI_cyto_J4024_model E (#29.5) to map |
21724 | | W20_J4024_consensus_resampled.mrc (#12) using 1876 atoms |
21725 | | average map value = 0.2646, steps = 56 |
21726 | | shifted from previous position = 0.686 |
21727 | | rotated from previous position = 1.54 degrees |
21728 | | atoms outside contour = 791, contour level = 0.25569 |
21729 | | |
21730 | | Position of COPI_cyto_J4024_model E (#29.5) relative to |
21731 | | W20_J4024_consensus_resampled.mrc (#12) coordinates: |
21732 | | Matrix rotation and translation |
21733 | | -0.38725918 -0.90565201 -0.17269853 278.00845664 |
21734 | | -0.78536225 0.22592118 0.57633824 161.81751759 |
21735 | | -0.48294563 0.35882318 -0.79875495 241.37260249 |
21736 | | Axis -0.54715674 0.78042314 0.30258755 |
21737 | | Axis point 210.33473059 0.00000000 69.77477808 |
21738 | | Rotation angle (degrees) 168.53504549 |
21739 | | Shift along axis 47.20827915 |
21740 | | |
21741 | | |
21742 | | > fitmap #29.6 inMap #12 |
21743 | | |
21744 | | Fit molecule COPI_cyto_J4024_model F (#29.6) to map |
21745 | | W20_J4024_consensus_resampled.mrc (#12) using 2400 atoms |
21746 | | average map value = 0.242, steps = 84 |
21747 | | shifted from previous position = 5.87 |
21748 | | rotated from previous position = 13.7 degrees |
21749 | | atoms outside contour = 1297, contour level = 0.25569 |
21750 | | |
21751 | | Position of COPI_cyto_J4024_model F (#29.6) relative to |
21752 | | W20_J4024_consensus_resampled.mrc (#12) coordinates: |
21753 | | Matrix rotation and translation |
21754 | | -0.53159589 -0.84531226 -0.05341354 266.88246547 |
21755 | | -0.64371973 0.36222400 0.67410585 127.52327824 |
21756 | | -0.55048227 0.39273525 -0.73670097 230.79457007 |
21757 | | Axis -0.46452916 0.82063626 0.33281944 |
21758 | | Axis point 189.53211141 0.00000000 61.79948623 |
21759 | | Rotation angle (degrees) 162.37079623 |
21760 | | Shift along axis 57.48845832 |
21761 | | |
21762 | | |
21763 | | > fitmap #29.7 inMap #12 |
21764 | | |
21765 | | Fit molecule COPI_cyto_J4024_model G (#29.7) to map |
21766 | | W20_J4024_consensus_resampled.mrc (#12) using 2302 atoms |
21767 | | average map value = 0.2775, steps = 60 |
21768 | | shifted from previous position = 3.1 |
21769 | | rotated from previous position = 4.14 degrees |
21770 | | atoms outside contour = 948, contour level = 0.25569 |
21771 | | |
21772 | | Position of COPI_cyto_J4024_model G (#29.7) relative to |
21773 | | W20_J4024_consensus_resampled.mrc (#12) coordinates: |
21774 | | Matrix rotation and translation |
21775 | | -0.36698963 -0.92232957 -0.12094124 274.70465819 |
21776 | | -0.76065109 0.22270273 0.60976506 158.64044193 |
21777 | | -0.53547040 0.31577154 -0.78330058 243.71016364 |
21778 | | Axis -0.55127458 0.77729395 0.30316736 |
21779 | | Axis point 213.68280008 0.00000000 64.72109245 |
21780 | | Rotation angle (degrees) 164.53504616 |
21781 | | Shift along axis 45.75752709 |
21782 | | |
21783 | | |
21784 | | > fitmap #29.8 inMap #12 |
21785 | | |
21786 | | Fit molecule COPI_cyto_J4024_model H (#29.8) to map |
21787 | | W20_J4024_consensus_resampled.mrc (#12) using 420 atoms |
21788 | | average map value = 0.3642, steps = 52 |
21789 | | shifted from previous position = 3.12 |
21790 | | rotated from previous position = 7.89 degrees |
21791 | | atoms outside contour = 112, contour level = 0.25569 |
21792 | | |
21793 | | Position of COPI_cyto_J4024_model H (#29.8) relative to |
21794 | | W20_J4024_consensus_resampled.mrc (#12) coordinates: |
21795 | | Matrix rotation and translation |
21796 | | -0.31100107 -0.94914560 -0.04899985 269.58279474 |
21797 | | -0.77806832 0.22466013 0.58663236 161.32624746 |
21798 | | -0.54579120 0.22056852 -0.80836966 258.53074932 |
21799 | | Axis -0.57164561 0.77578980 0.26715442 |
21800 | | Axis point 220.48916877 0.00000000 70.49015049 |
21801 | | Rotation angle (degrees) 161.32593230 |
21802 | | Shift along axis 40.11707004 |
21803 | | |
21804 | | |
21805 | | > fitmap #29.9 inMap #12 |
21806 | | |
21807 | | Fit molecule COPI_cyto_J4024_model I (#29.9) to map |
21808 | | W20_J4024_consensus_resampled.mrc (#12) using 1573 atoms |
21809 | | average map value = 0.308, steps = 88 |
21810 | | shifted from previous position = 1.06 |
21811 | | rotated from previous position = 3.68 degrees |
21812 | | atoms outside contour = 615, contour level = 0.25569 |
21813 | | |
21814 | | Position of COPI_cyto_J4024_model I (#29.9) relative to |
21815 | | W20_J4024_consensus_resampled.mrc (#12) coordinates: |
21816 | | Matrix rotation and translation |
21817 | | -0.32207758 -0.93636157 -0.13961755 272.41905490 |
21818 | | -0.79316925 0.18637492 0.57978181 166.78930021 |
21819 | | -0.51686418 0.29747507 -0.80272038 248.24026118 |
21820 | | Axis -0.57325903 0.76604633 0.29076985 |
21821 | | Axis point 217.83744421 0.00000000 68.18432836 |
21822 | | Rotation angle (degrees) 165.74548655 |
21823 | | Shift along axis 43.78243158 |
21824 | | |
21825 | | |
21826 | | > fitmap #29.10 inMap #12 |
21827 | | |
21828 | | Fit molecule COPI_cyto_J4024_model J (#29.10) to map |
21829 | | W20_J4024_consensus_resampled.mrc (#12) using 1112 atoms |
21830 | | average map value = 0.3363, steps = 72 |
21831 | | shifted from previous position = 0.618 |
21832 | | rotated from previous position = 4.66 degrees |
21833 | | atoms outside contour = 406, contour level = 0.25569 |
21834 | | |
21835 | | Position of COPI_cyto_J4024_model J (#29.10) relative to |
21836 | | W20_J4024_consensus_resampled.mrc (#12) coordinates: |
21837 | | Matrix rotation and translation |
21838 | | -0.36498462 -0.92690394 -0.08738035 267.73871712 |
21839 | | -0.79378094 0.26076630 0.54946590 162.75689323 |
21840 | | -0.48651625 0.26990746 -0.83093436 251.13764208 |
21841 | | Axis -0.55340845 0.79012166 0.26352770 |
21842 | | Axis point 210.27082462 0.00000000 74.58174748 |
21843 | | Rotation angle (degrees) 165.36983736 |
21844 | | Shift along axis 46.61060410 |
21845 | | |
21846 | | |
21847 | | > hide #!12 models |
21848 | | |
21849 | | > hide #!29 models |
21850 | | |
21851 | | > show #!30 models |
21852 | | |
21853 | | > split #30 chains |
21854 | | |
21855 | | Split COPI_cyto_J4025_model (#30) into 13 models |
21856 | | Chain information for COPI_cyto_J4025_model A #30.1 |
21857 | | --- |
21858 | | Chain | Description |
21859 | | A | No description available |
21860 | | |
21861 | | Chain information for COPI_cyto_J4025_model B #30.2 |
21862 | | --- |
21863 | | Chain | Description |
21864 | | B | No description available |
21865 | | |
21866 | | Chain information for COPI_cyto_J4025_model C #30.3 |
21867 | | --- |
21868 | | Chain | Description |
21869 | | C | No description available |
21870 | | |
21871 | | Chain information for COPI_cyto_J4025_model D #30.4 |
21872 | | --- |
21873 | | Chain | Description |
21874 | | D | No description available |
21875 | | |
21876 | | Chain information for COPI_cyto_J4025_model E #30.5 |
21877 | | --- |
21878 | | Chain | Description |
21879 | | E | No description available |
21880 | | |
21881 | | Chain information for COPI_cyto_J4025_model F #30.6 |
21882 | | --- |
21883 | | Chain | Description |
21884 | | F | No description available |
21885 | | |
21886 | | Chain information for COPI_cyto_J4025_model G #30.7 |
21887 | | --- |
21888 | | Chain | Description |
21889 | | G | No description available |
21890 | | |
21891 | | Chain information for COPI_cyto_J4025_model H #30.8 |
21892 | | --- |
21893 | | Chain | Description |
21894 | | H | No description available |
21895 | | |
21896 | | Chain information for COPI_cyto_J4025_model I #30.9 |
21897 | | --- |
21898 | | Chain | Description |
21899 | | I | No description available |
21900 | | |
21901 | | Chain information for COPI_cyto_J4025_model J #30.10 |
21902 | | --- |
21903 | | Chain | Description |
21904 | | J | No description available |
21905 | | |
21906 | | Chain information for COPI_cyto_J4025_model K #30.11 |
21907 | | --- |
21908 | | Chain | Description |
21909 | | K | No description available |
21910 | | |
21911 | | Chain information for COPI_cyto_J4025_model L #30.12 |
21912 | | --- |
21913 | | Chain | Description |
21914 | | L | No description available |
21915 | | |
21916 | | Chain information for COPI_cyto_J4025_model M #30.13 |
21917 | | --- |
21918 | | Chain | Description |
21919 | | M | No description available |
21920 | | |
21921 | | |
21922 | | > show #!14 models |
21923 | | |
21924 | | > color #14 darkgrey models |
21925 | | |
21926 | | > color #14 #a9a9a9be models |
21927 | | |
21928 | | > color #14 #a9a9a9bf models |
21929 | | |
21930 | | > fitmap #30.1 inMap #14 |
21931 | | |
21932 | | Fit molecule COPI_cyto_J4025_model A (#30.1) to map |
21933 | | W20_J4025_consensus_resampled.mrc (#14) using 2590 atoms |
21934 | | average map value = 0.2675, steps = 76 |
21935 | | shifted from previous position = 0.303 |
21936 | | rotated from previous position = 0.916 degrees |
21937 | | atoms outside contour = 971, contour level = 0.25978 |
21938 | | |
21939 | | Position of COPI_cyto_J4025_model A (#30.1) relative to |
21940 | | W20_J4025_consensus_resampled.mrc (#14) coordinates: |
21941 | | Matrix rotation and translation |
21942 | | -0.38101620 -0.91006695 -0.16310984 276.49091025 |
21943 | | -0.78874603 0.22789915 0.57091304 162.02364661 |
21944 | | -0.48239649 0.34617935 -0.80464495 242.12831797 |
21945 | | Axis -0.54965686 0.78091585 0.29672844 |
21946 | | Axis point 210.37135033 0.00000000 70.30651243 |
21947 | | Rotation angle (degrees) 168.20381385 |
21948 | | Shift along axis 46.39806523 |
21949 | | |
21950 | | |
21951 | | > fitmap #30.2 inMap #14 |
21952 | | |
21953 | | Fit molecule COPI_cyto_J4025_model B (#30.2) to map |
21954 | | W20_J4025_consensus_resampled.mrc (#14) using 2426 atoms |
21955 | | average map value = 0.2464, steps = 56 |
21956 | | shifted from previous position = 0.713 |
21957 | | rotated from previous position = 1.44 degrees |
21958 | | atoms outside contour = 1409, contour level = 0.25978 |
21959 | | |
21960 | | Position of COPI_cyto_J4025_model B (#30.2) relative to |
21961 | | W20_J4025_consensus_resampled.mrc (#14) coordinates: |
21962 | | Matrix rotation and translation |
21963 | | -0.37124088 -0.90903393 -0.18930807 277.53484582 |
21964 | | -0.80004519 0.20967074 0.56210843 165.71657123 |
21965 | | -0.47128327 0.36013264 -0.80510656 240.05521807 |
21966 | | Axis -0.55553559 0.77557441 0.29977415 |
21967 | | Axis point 211.48229973 0.00000000 70.08195116 |
21968 | | Rotation angle (degrees) 169.52626651 |
21969 | | Shift along axis 46.30739501 |
21970 | | |
21971 | | |
21972 | | > fitmap #30.3 inMap #14 |
21973 | | |
21974 | | Fit molecule COPI_cyto_J4025_model C (#30.3) to map |
21975 | | W20_J4025_consensus_resampled.mrc (#14) using 1612 atoms |
21976 | | average map value = 0.2632, steps = 96 |
21977 | | shifted from previous position = 1.92 |
21978 | | rotated from previous position = 10.7 degrees |
21979 | | atoms outside contour = 694, contour level = 0.25978 |
21980 | | |
21981 | | Position of COPI_cyto_J4025_model C (#30.3) relative to |
21982 | | W20_J4025_consensus_resampled.mrc (#14) coordinates: |
21983 | | Matrix rotation and translation |
21984 | | -0.44316515 -0.89625169 -0.01837289 273.18677871 |
21985 | | -0.69089200 0.32841899 0.64405685 142.84601321 |
21986 | | -0.57120303 0.29811723 -0.76475697 249.76514069 |
21987 | | Axis -0.50596953 0.80856656 0.30035804 |
21988 | | Axis point 206.79517933 0.00000000 65.69504944 |
21989 | | Rotation angle (degrees) 160.00987675 |
21990 | | Shift along axis 52.29529270 |
21991 | | |
21992 | | |
21993 | | > fitmap #30.4 inMap #14 |
21994 | | |
21995 | | Fit molecule COPI_cyto_J4025_model D (#30.4) to map |
21996 | | W20_J4025_consensus_resampled.mrc (#14) using 4745 atoms |
21997 | | average map value = 0.2994, steps = 96 |
21998 | | shifted from previous position = 0.519 |
21999 | | rotated from previous position = 1.3 degrees |
22000 | | atoms outside contour = 1727, contour level = 0.25978 |
22001 | | |
22002 | | Position of COPI_cyto_J4025_model D (#30.4) relative to |
22003 | | W20_J4025_consensus_resampled.mrc (#14) coordinates: |
22004 | | Matrix rotation and translation |
22005 | | -0.37140408 -0.91616900 -0.15064322 274.98034054 |
22006 | | -0.80234855 0.23505413 0.54862225 164.28861064 |
22007 | | -0.46722138 0.32462891 -0.82238693 243.65233050 |
22008 | | Axis -0.55421176 0.78328822 0.28161834 |
22009 | | Axis point 210.66577055 0.00000000 72.75003830 |
22010 | | Rotation angle (degrees) 168.34121758 |
22011 | | Shift along axis 44.90495816 |
22012 | | |
22013 | | |
22014 | | > fitmap #30.5 inMap #14 |
22015 | | |
22016 | | Fit molecule COPI_cyto_J4025_model E (#30.5) to map |
22017 | | W20_J4025_consensus_resampled.mrc (#14) using 1876 atoms |
22018 | | average map value = 0.2637, steps = 64 |
22019 | | shifted from previous position = 0.305 |
22020 | | rotated from previous position = 1.58 degrees |
22021 | | atoms outside contour = 846, contour level = 0.25978 |
22022 | | |
22023 | | Position of COPI_cyto_J4025_model E (#30.5) relative to |
22024 | | W20_J4025_consensus_resampled.mrc (#14) coordinates: |
22025 | | Matrix rotation and translation |
22026 | | -0.38354345 -0.90936875 -0.16106801 276.77746085 |
22027 | | -0.78111798 0.22638522 0.58189729 160.60505785 |
22028 | | -0.49269579 0.34899601 -0.79715284 242.75004629 |
22029 | | Axis -0.54793741 0.78020725 0.30173041 |
22030 | | Axis point 210.53234092 0.00000000 69.48347841 |
22031 | | Rotation angle (degrees) 167.72960946 |
22032 | | Shift along axis 46.89357823 |
22033 | | |
22034 | | |
22035 | | > fitmap #30.6 inMap #14 |
22036 | | |
22037 | | Fit molecule COPI_cyto_J4025_model F (#30.6) to map |
22038 | | W20_J4025_consensus_resampled.mrc (#14) using 2400 atoms |
22039 | | average map value = 0.3154, steps = 76 |
22040 | | shifted from previous position = 4.38 |
22041 | | rotated from previous position = 6.32 degrees |
22042 | | atoms outside contour = 716, contour level = 0.25978 |
22043 | | |
22044 | | Position of COPI_cyto_J4025_model F (#30.6) relative to |
22045 | | W20_J4025_consensus_resampled.mrc (#14) coordinates: |
22046 | | Matrix rotation and translation |
22047 | | -0.46916307 -0.86826583 -0.16124662 276.76831771 |
22048 | | -0.75844576 0.30262715 0.57721474 152.99334716 |
22049 | | -0.45237823 0.39310465 -0.80051401 236.07204224 |
22050 | | Axis -0.50923185 0.80524369 0.30375239 |
22051 | | Axis point 199.19186600 0.00000000 71.96275109 |
22052 | | Rotation angle (degrees) 169.58524914 |
22053 | | Shift along axis 53.96513192 |
22054 | | |
22055 | | |
22056 | | > fitmap #30.7 inMap #14 |
22057 | | |
22058 | | Fit molecule COPI_cyto_J4025_model G (#30.7) to map |
22059 | | W20_J4025_consensus_resampled.mrc (#14) using 2302 atoms |
22060 | | average map value = 0.2919, steps = 76 |
22061 | | shifted from previous position = 4.85 |
22062 | | rotated from previous position = 4.34 degrees |
22063 | | atoms outside contour = 830, contour level = 0.25978 |
22064 | | |
22065 | | Position of COPI_cyto_J4025_model G (#30.7) relative to |
22066 | | W20_J4025_consensus_resampled.mrc (#14) coordinates: |
22067 | | Matrix rotation and translation |
22068 | | -0.43043084 -0.88252203 -0.18943119 279.00638393 |
22069 | | -0.77202835 0.25122185 0.58383201 159.76771478 |
22070 | | -0.46765535 0.39754556 -0.78946565 235.51132473 |
22071 | | Axis -0.52833633 0.78908543 0.31337663 |
22072 | | Axis point 205.32244175 0.00000000 68.76349381 |
22073 | | Rotation angle (degrees) 169.84595276 |
22074 | | Shift along axis 52.46491169 |
22075 | | |
22076 | | |
22077 | | > fitmap #30.8 inMap #14 |
22078 | | |
22079 | | Fit molecule COPI_cyto_J4025_model H (#30.8) to map |
22080 | | W20_J4025_consensus_resampled.mrc (#14) using 420 atoms |
22081 | | average map value = 0.3536, steps = 80 |
22082 | | shifted from previous position = 3.69 |
22083 | | rotated from previous position = 5.02 degrees |
22084 | | atoms outside contour = 122, contour level = 0.25978 |
22085 | | |
22086 | | Position of COPI_cyto_J4025_model H (#30.8) relative to |
22087 | | W20_J4025_consensus_resampled.mrc (#14) coordinates: |
22088 | | Matrix rotation and translation |
22089 | | -0.35116806 -0.91592354 -0.19433237 278.04382885 |
22090 | | -0.84420748 0.21996160 0.48880532 174.65406664 |
22091 | | -0.40496263 0.33570965 -0.85047299 242.78401221 |
22092 | | Axis -0.56683990 0.77986296 0.26553022 |
22093 | | Axis point 212.55649174 0.00000000 78.50487362 |
22094 | | Rotation angle (degrees) 172.23888649 |
22095 | | Shift along axis 43.06639339 |
22096 | | |
22097 | | |
22098 | | > fitmap #30.9 inMap #14 |
22099 | | |
22100 | | Fit molecule COPI_cyto_J4025_model I (#30.9) to map |
22101 | | W20_J4025_consensus_resampled.mrc (#14) using 1573 atoms |
22102 | | average map value = 0.2801, steps = 68 |
22103 | | shifted from previous position = 1.75 |
22104 | | rotated from previous position = 3.58 degrees |
22105 | | atoms outside contour = 701, contour level = 0.25978 |
22106 | | |
22107 | | Position of COPI_cyto_J4025_model I (#30.9) relative to |
22108 | | W20_J4025_consensus_resampled.mrc (#14) coordinates: |
22109 | | Matrix rotation and translation |
22110 | | -0.38742239 -0.89858889 -0.20601436 277.54357463 |
22111 | | -0.78023576 0.20056569 0.59245724 165.02578227 |
22112 | | -0.49105608 0.39027097 -0.77881481 238.87427461 |
22113 | | Axis -0.54798153 0.77254303 0.32077019 |
22114 | | Axis point 210.67928153 0.00000000 66.74726373 |
22115 | | Rotation angle (degrees) 169.36902321 |
22116 | | Shift along axis 52.02451216 |
22117 | | |
22118 | | |
22119 | | > fitmap #30.10 inMap #14 |
22120 | | |
22121 | | Fit molecule COPI_cyto_J4025_model J (#30.10) to map |
22122 | | W20_J4025_consensus_resampled.mrc (#14) using 1112 atoms |
22123 | | average map value = 0.3297, steps = 72 |
22124 | | shifted from previous position = 0.309 |
22125 | | rotated from previous position = 4.19 degrees |
22126 | | atoms outside contour = 404, contour level = 0.25978 |
22127 | | |
22128 | | Position of COPI_cyto_J4025_model J (#30.10) relative to |
22129 | | W20_J4025_consensus_resampled.mrc (#14) coordinates: |
22130 | | Matrix rotation and translation |
22131 | | -0.35980949 -0.92767682 -0.09976404 268.82850280 |
22132 | | -0.80210592 0.25292807 0.54097459 164.87500239 |
22133 | | -0.47661645 0.27466912 -0.83510098 249.92805127 |
22134 | | Axis -0.55685591 0.78801420 0.26257402 |
22135 | | Axis point 210.98119167 0.00000000 74.83620925 |
22136 | | Rotation angle (degrees) 166.16567308 |
22137 | | Shift along axis 45.84971671 |
22138 | | |
22139 | | |
22140 | | > hide #!30 models |
22141 | | |
22142 | | > split #31 chains |
22143 | | |
22144 | | Split COPI_cyto_J4026_model (#31) into 13 models |
22145 | | Chain information for COPI_cyto_J4026_model A #31.1 |
22146 | | --- |
22147 | | Chain | Description |
22148 | | A | No description available |
22149 | | |
22150 | | Chain information for COPI_cyto_J4026_model B #31.2 |
22151 | | --- |
22152 | | Chain | Description |
22153 | | B | No description available |
22154 | | |
22155 | | Chain information for COPI_cyto_J4026_model C #31.3 |
22156 | | --- |
22157 | | Chain | Description |
22158 | | C | No description available |
22159 | | |
22160 | | Chain information for COPI_cyto_J4026_model D #31.4 |
22161 | | --- |
22162 | | Chain | Description |
22163 | | D | No description available |
22164 | | |
22165 | | Chain information for COPI_cyto_J4026_model E #31.5 |
22166 | | --- |
22167 | | Chain | Description |
22168 | | E | No description available |
22169 | | |
22170 | | Chain information for COPI_cyto_J4026_model F #31.6 |
22171 | | --- |
22172 | | Chain | Description |
22173 | | F | No description available |
22174 | | |
22175 | | Chain information for COPI_cyto_J4026_model G #31.7 |
22176 | | --- |
22177 | | Chain | Description |
22178 | | G | No description available |
22179 | | |
22180 | | Chain information for COPI_cyto_J4026_model H #31.8 |
22181 | | --- |
22182 | | Chain | Description |
22183 | | H | No description available |
22184 | | |
22185 | | Chain information for COPI_cyto_J4026_model I #31.9 |
22186 | | --- |
22187 | | Chain | Description |
22188 | | I | No description available |
22189 | | |
22190 | | Chain information for COPI_cyto_J4026_model J #31.10 |
22191 | | --- |
22192 | | Chain | Description |
22193 | | J | No description available |
22194 | | |
22195 | | Chain information for COPI_cyto_J4026_model K #31.11 |
22196 | | --- |
22197 | | Chain | Description |
22198 | | K | No description available |
22199 | | |
22200 | | Chain information for COPI_cyto_J4026_model L #31.12 |
22201 | | --- |
22202 | | Chain | Description |
22203 | | L | No description available |
22204 | | |
22205 | | Chain information for COPI_cyto_J4026_model M #31.13 |
22206 | | --- |
22207 | | Chain | Description |
22208 | | M | No description available |
22209 | | |
22210 | | |
22211 | | > show #31.1 models |
22212 | | |
22213 | | > show #31.2 models |
22214 | | |
22215 | | > show #31.3 models |
22216 | | |
22217 | | > show #!31.4 models |
22218 | | |
22219 | | > show #31.5 models |
22220 | | |
22221 | | > show #31.6 models |
22222 | | |
22223 | | > show #31.7 models |
22224 | | |
22225 | | > show #31.8 models |
22226 | | |
22227 | | > show #31.9 models |
22228 | | |
22229 | | > show #31.10 models |
22230 | | |
22231 | | > show #31.11 models |
22232 | | |
22233 | | > show #31.12 models |
22234 | | |
22235 | | > show #31.13 models |
22236 | | |
22237 | | > hide #!14 models |
22238 | | |
22239 | | > color #16 darkgrey models |
22240 | | |
22241 | | > color #16 #a9a9a9bf models |
22242 | | |
22243 | | > show #!16 models |
22244 | | |
22245 | | > fitmap #31.1 inMap #16 |
22246 | | |
22247 | | Fit molecule COPI_cyto_J4026_model A (#31.1) to map |
22248 | | W20_J4026_consensus_resampled.mrc (#16) using 2590 atoms |
22249 | | average map value = 0.3888, steps = 44 |
22250 | | shifted from previous position = 1.29 |
22251 | | rotated from previous position = 3.47 degrees |
22252 | | atoms outside contour = 601, contour level = 0.25963 |
22253 | | |
22254 | | Position of COPI_cyto_J4026_model A (#31.1) relative to |
22255 | | W20_J4026_consensus_resampled.mrc (#16) coordinates: |
22256 | | Matrix rotation and translation |
22257 | | -0.42179573 -0.88868235 -0.17981118 281.30883739 |
22258 | | -0.78142467 0.25572018 0.56919478 159.93267883 |
22259 | | -0.45985201 0.38059282 -0.80229998 237.51658930 |
22260 | | Axis -0.53238793 0.79050266 0.30276829 |
22261 | | Axis point 207.09091155 0.00000000 70.78659039 |
22262 | | Rotation angle (degrees) 169.79746249 |
22263 | | Shift along axis 48.57427120 |
22264 | | |
22265 | | |
22266 | | > fitmap #31.2 inMap #16 |
22267 | | |
22268 | | Fit molecule COPI_cyto_J4026_model B (#31.2) to map |
22269 | | W20_J4026_consensus_resampled.mrc (#16) using 2426 atoms |
22270 | | average map value = 0.3729, steps = 96 |
22271 | | shifted from previous position = 1.87 |
22272 | | rotated from previous position = 3.62 degrees |
22273 | | atoms outside contour = 599, contour level = 0.25963 |
22274 | | |
22275 | | Position of COPI_cyto_J4026_model B (#31.2) relative to |
22276 | | W20_J4026_consensus_resampled.mrc (#16) coordinates: |
22277 | | Matrix rotation and translation |
22278 | | -0.41831806 -0.88734205 -0.19399514 282.19270881 |
22279 | | -0.78598077 0.24658443 0.56694830 161.68081011 |
22280 | | -0.45524088 0.38964115 -0.80058449 236.37167847 |
22281 | | Axis -0.53469530 0.78782425 0.30566958 |
22282 | | Axis point 207.69640372 0.00000000 70.48893010 |
22283 | | Rotation angle (degrees) 170.45617020 |
22284 | | Shift along axis 48.74057955 |
22285 | | |
22286 | | |
22287 | | > fitmap #31.3 inMap #16 |
22288 | | |
22289 | | Fit molecule COPI_cyto_J4026_model C (#31.3) to map |
22290 | | W20_J4026_consensus_resampled.mrc (#16) using 1612 atoms |
22291 | | average map value = 0.3193, steps = 132 |
22292 | | shifted from previous position = 1.47 |
22293 | | rotated from previous position = 4.65 degrees |
22294 | | atoms outside contour = 561, contour level = 0.25963 |
22295 | | |
22296 | | Position of COPI_cyto_J4026_model C (#31.3) relative to |
22297 | | W20_J4026_consensus_resampled.mrc (#16) coordinates: |
22298 | | Matrix rotation and translation |
22299 | | -0.38059936 -0.91824851 -0.10937920 274.00313195 |
22300 | | -0.75305941 0.23911656 0.61296396 156.01911017 |
22301 | | -0.53669887 0.31566272 -0.78250328 246.73459431 |
22302 | | Axis -0.54436027 0.78242476 0.30246220 |
22303 | | Axis point 211.94174234 0.00000000 66.53857749 |
22304 | | Rotation angle (degrees) 164.15273201 |
22305 | | Shift along axis 47.54468445 |
22306 | | |
22307 | | |
22308 | | > fitmap #31.4 inMap #16 |
22309 | | |
22310 | | Fit molecule COPI_cyto_J4026_model D (#31.4) to map |
22311 | | W20_J4026_consensus_resampled.mrc (#16) using 4745 atoms |
22312 | | average map value = 0.2725, steps = 48 |
22313 | | shifted from previous position = 0.247 |
22314 | | rotated from previous position = 2.27 degrees |
22315 | | atoms outside contour = 1838, contour level = 0.25963 |
22316 | | |
22317 | | Position of COPI_cyto_J4026_model D (#31.4) relative to |
22318 | | W20_J4026_consensus_resampled.mrc (#16) coordinates: |
22319 | | Matrix rotation and translation |
22320 | | -0.36032627 -0.92283158 -0.13618688 272.90164046 |
22321 | | -0.80657650 0.23487967 0.54246282 165.85713835 |
22322 | | -0.46861429 0.30530874 -0.82896757 246.32646884 |
22323 | | Axis -0.55856608 0.78296216 0.27381417 |
22324 | | Axis point 211.65908561 0.00000000 73.73169378 |
22325 | | Rotation angle (degrees) 167.74351385 |
22326 | | Shift along axis 44.87394129 |
22327 | | |
22328 | | |
22329 | | > fitmap #31.5 inMap #16 |
22330 | | |
22331 | | Fit molecule COPI_cyto_J4026_model E (#31.5) to map |
22332 | | W20_J4026_consensus_resampled.mrc (#16) using 1876 atoms |
22333 | | average map value = 0.3337, steps = 68 |
22334 | | shifted from previous position = 0.775 |
22335 | | rotated from previous position = 3.95 degrees |
22336 | | atoms outside contour = 584, contour level = 0.25963 |
22337 | | |
22338 | | Position of COPI_cyto_J4026_model E (#31.5) relative to |
22339 | | W20_J4026_consensus_resampled.mrc (#16) coordinates: |
22340 | | Matrix rotation and translation |
22341 | | -0.41217203 -0.89272092 -0.18210871 281.24771125 |
22342 | | -0.76396069 0.22971791 0.60298736 156.90992899 |
22343 | | -0.49646580 0.38765842 -0.77668699 239.04221312 |
22344 | | Axis -0.53537564 0.78159084 0.32013853 |
22345 | | Axis point 208.88799304 0.00000000 67.41763702 |
22346 | | Rotation angle (degrees) 168.39866163 |
22347 | | Shift along axis 48.59281177 |
22348 | | |
22349 | | |
22350 | | > fitmap #31.10 inMap #16 |
22351 | | |
22352 | | Fit molecule COPI_cyto_J4026_model J (#31.10) to map |
22353 | | W20_J4026_consensus_resampled.mrc (#16) using 1112 atoms |
22354 | | average map value = 0.275, steps = 132 |
22355 | | shifted from previous position = 1.13 |
22356 | | rotated from previous position = 6.46 degrees |
22357 | | atoms outside contour = 480, contour level = 0.25963 |
22358 | | |
22359 | | Position of COPI_cyto_J4026_model J (#31.10) relative to |
22360 | | W20_J4026_consensus_resampled.mrc (#16) coordinates: |
22361 | | Matrix rotation and translation |
22362 | | -0.39165506 -0.91840010 -0.05610338 267.15064982 |
22363 | | -0.77630145 0.29709402 0.55595973 158.01112550 |
22364 | | -0.49392549 0.26129757 -0.82931369 252.31232484 |
22365 | | Axis -0.53914103 0.80107967 0.25999678 |
22366 | | Axis point 207.59429240 0.00000000 75.03244785 |
22367 | | Rotation angle (degrees) 164.14105481 |
22368 | | Shift along axis 48.14801546 |
22369 | | |
22370 | | |
22371 | | > fitmap #31.10 inMap #16 |
22372 | | |
22373 | | Fit molecule COPI_cyto_J4026_model J (#31.10) to map |
22374 | | W20_J4026_consensus_resampled.mrc (#16) using 1112 atoms |
22375 | | average map value = 0.275, steps = 48 |
22376 | | shifted from previous position = 0.0181 |
22377 | | rotated from previous position = 0.0479 degrees |
22378 | | atoms outside contour = 481, contour level = 0.25963 |
22379 | | |
22380 | | Position of COPI_cyto_J4026_model J (#31.10) relative to |
22381 | | W20_J4026_consensus_resampled.mrc (#16) coordinates: |
22382 | | Matrix rotation and translation |
22383 | | -0.39111474 -0.91864714 -0.05582756 267.08423778 |
22384 | | -0.77617700 0.29664639 0.55637235 158.00648729 |
22385 | | -0.49454882 0.26093749 -0.82905555 252.36987731 |
22386 | | Axis -0.53933760 0.80091724 0.26008948 |
22387 | | Axis point 207.64765628 0.00000000 74.98798493 |
22388 | | Rotation angle (degrees) 164.10431634 |
22389 | | Shift along axis 48.14029770 |
22390 | | |
22391 | | |
22392 | | > fitmap #31.12 inMap #16 |
22393 | | |
22394 | | Fit molecule COPI_cyto_J4026_model L (#31.12) to map |
22395 | | W20_J4026_consensus_resampled.mrc (#16) using 1420 atoms |
22396 | | average map value = 0.246, steps = 148 |
22397 | | shifted from previous position = 18 |
22398 | | rotated from previous position = 21.2 degrees |
22399 | | atoms outside contour = 781, contour level = 0.25963 |
22400 | | |
22401 | | Position of COPI_cyto_J4026_model L (#31.12) relative to |
22402 | | W20_J4026_consensus_resampled.mrc (#16) coordinates: |
22403 | | Matrix rotation and translation |
22404 | | -0.20471014 -0.86077408 -0.46600619 296.00976398 |
22405 | | -0.82456258 -0.10488916 0.55596297 187.90289621 |
22406 | | -0.52743751 0.49806252 -0.68829022 207.63206185 |
22407 | | Axis -0.63033876 0.66877783 0.39421981 |
22408 | | Axis point 239.98122179 0.00000000 43.12371251 |
22409 | | Rotation angle (degrees) 177.36759071 |
22410 | | Shift along axis 20.93153367 |
22411 | | |
22412 | | |
22413 | | > fitmap #31.13 inMap #16 |
22414 | | |
22415 | | Fit molecule COPI_cyto_J4026_model M (#31.13) to map |
22416 | | W20_J4026_consensus_resampled.mrc (#16) using 4552 atoms |
22417 | | average map value = 0.2784, steps = 240 |
22418 | | shifted from previous position = 13.3 |
22419 | | rotated from previous position = 28.4 degrees |
22420 | | atoms outside contour = 2174, contour level = 0.25963 |
22421 | | |
22422 | | Position of COPI_cyto_J4026_model M (#31.13) relative to |
22423 | | W20_J4026_consensus_resampled.mrc (#16) coordinates: |
22424 | | Matrix rotation and translation |
22425 | | -0.19059508 -0.97763486 0.08890226 232.38163718 |
22426 | | -0.95481798 0.20565637 0.21454160 224.73228555 |
22427 | | -0.22802666 -0.04399490 -0.97266043 250.13777446 |
22428 | | Axis -0.63112960 0.77367498 0.05569971 |
22429 | | Axis point 224.43338633 0.00000000 99.69760848 |
22430 | | Rotation angle (degrees) 168.18101146 |
22431 | | Shift along axis 41.13941809 |
22432 | | |
22433 | | |
22434 | | > hide #!31 models |
22435 | | |
22436 | | > show #!32 models |
22437 | | |
22438 | | > split #32 chains |
22439 | | |
22440 | | Split COPI_cyto_J4027_model (#32) into 13 models |
22441 | | Chain information for COPI_cyto_J4027_model A #32.1 |
22442 | | --- |
22443 | | Chain | Description |
22444 | | A | No description available |
22445 | | |
22446 | | Chain information for COPI_cyto_J4027_model B #32.2 |
22447 | | --- |
22448 | | Chain | Description |
22449 | | B | No description available |
22450 | | |
22451 | | Chain information for COPI_cyto_J4027_model C #32.3 |
22452 | | --- |
22453 | | Chain | Description |
22454 | | C | No description available |
22455 | | |
22456 | | Chain information for COPI_cyto_J4027_model D #32.4 |
22457 | | --- |
22458 | | Chain | Description |
22459 | | D | No description available |
22460 | | |
22461 | | Chain information for COPI_cyto_J4027_model E #32.5 |
22462 | | --- |
22463 | | Chain | Description |
22464 | | E | No description available |
22465 | | |
22466 | | Chain information for COPI_cyto_J4027_model F #32.6 |
22467 | | --- |
22468 | | Chain | Description |
22469 | | F | No description available |
22470 | | |
22471 | | Chain information for COPI_cyto_J4027_model G #32.7 |
22472 | | --- |
22473 | | Chain | Description |
22474 | | G | No description available |
22475 | | |
22476 | | Chain information for COPI_cyto_J4027_model H #32.8 |
22477 | | --- |
22478 | | Chain | Description |
22479 | | H | No description available |
22480 | | |
22481 | | Chain information for COPI_cyto_J4027_model I #32.9 |
22482 | | --- |
22483 | | Chain | Description |
22484 | | I | No description available |
22485 | | |
22486 | | Chain information for COPI_cyto_J4027_model J #32.10 |
22487 | | --- |
22488 | | Chain | Description |
22489 | | J | No description available |
22490 | | |
22491 | | Chain information for COPI_cyto_J4027_model K #32.11 |
22492 | | --- |
22493 | | Chain | Description |
22494 | | K | No description available |
22495 | | |
22496 | | Chain information for COPI_cyto_J4027_model L #32.12 |
22497 | | --- |
22498 | | Chain | Description |
22499 | | L | No description available |
22500 | | |
22501 | | Chain information for COPI_cyto_J4027_model M #32.13 |
22502 | | --- |
22503 | | Chain | Description |
22504 | | M | No description available |
22505 | | |
22506 | | |
22507 | | > hide #!16 models |
22508 | | |
22509 | | > show #!18 models |
22510 | | |
22511 | | > color #18 darkgrey models |
22512 | | |
22513 | | > color #18 #a9a9a9be models |
22514 | | |
22515 | | > color #18 #a9a9a9bf models |
22516 | | |
22517 | | > fitmap #32.1 inMap #18 |
22518 | | |
22519 | | Fit molecule COPI_cyto_J4027_model A (#32.1) to map |
22520 | | W20_J4027_consensus_resampled.mrc (#18) using 2590 atoms |
22521 | | average map value = 0.3669, steps = 68 |
22522 | | shifted from previous position = 0.35 |
22523 | | rotated from previous position = 0.41 degrees |
22524 | | atoms outside contour = 652, contour level = 0.25259 |
22525 | | |
22526 | | Position of COPI_cyto_J4027_model A (#32.1) relative to |
22527 | | W20_J4027_consensus_resampled.mrc (#18) coordinates: |
22528 | | Matrix rotation and translation |
22529 | | -0.36484420 -0.91715830 -0.16034147 275.00307794 |
22530 | | -0.79726014 0.21879492 0.56258783 164.15850506 |
22531 | | -0.48090019 0.33309077 -0.81103980 243.29869461 |
22532 | | Axis -0.55693597 0.77792142 0.29096492 |
22533 | | Axis point 211.66585714 0.00000000 70.85307433 |
22534 | | Rotation angle (degrees) 168.10988229 |
22535 | | Shift along axis 45.33469685 |
22536 | | |
22537 | | |
22538 | | > fitmap #32.2 inMap #18 |
22539 | | |
22540 | | Fit molecule COPI_cyto_J4027_model B (#32.2) to map |
22541 | | W20_J4027_consensus_resampled.mrc (#18) using 2426 atoms |
22542 | | average map value = 0.354, steps = 64 |
22543 | | shifted from previous position = 0.279 |
22544 | | rotated from previous position = 0.915 degrees |
22545 | | atoms outside contour = 634, contour level = 0.25259 |
22546 | | |
22547 | | Position of COPI_cyto_J4027_model B (#32.2) relative to |
22548 | | W20_J4027_consensus_resampled.mrc (#18) coordinates: |
22549 | | Matrix rotation and translation |
22550 | | -0.37575375 -0.90933745 -0.17864639 277.57875572 |
22551 | | -0.79848110 0.21984249 0.56044378 165.00984098 |
22552 | | -0.47035845 0.35323462 -0.80869540 240.85491140 |
22553 | | Axis -0.55315660 0.77874186 0.29593731 |
22554 | | Axis point 211.17574622 0.00000000 70.68321054 |
22555 | | Rotation angle (degrees) 169.20491693 |
22556 | | Shift along axis 46.23350389 |
22557 | | |
22558 | | |
22559 | | > fitmap #32.3 inMap #18 |
22560 | | |
22561 | | Fit molecule COPI_cyto_J4027_model C (#32.3) to map |
22562 | | W20_J4027_consensus_resampled.mrc (#18) using 1612 atoms |
22563 | | average map value = 0.307, steps = 124 |
22564 | | shifted from previous position = 0.916 |
22565 | | rotated from previous position = 4.06 degrees |
22566 | | atoms outside contour = 549, contour level = 0.25259 |
22567 | | |
22568 | | Position of COPI_cyto_J4027_model C (#32.3) relative to |
22569 | | W20_J4027_consensus_resampled.mrc (#18) coordinates: |
22570 | | Matrix rotation and translation |
22571 | | -0.38280043 -0.91660120 -0.11535204 274.19904393 |
22572 | | -0.75859054 0.24060781 0.60551491 157.21505378 |
22573 | | -0.52726108 0.31929633 -0.78742976 246.39824143 |
22574 | | Axis -0.54425835 0.78326479 0.30046485 |
22575 | | Axis point 211.53956724 0.00000000 67.39140608 |
22576 | | Rotation angle (degrees) 164.75519865 |
22577 | | Shift along axis 47.93990763 |
22578 | | |
22579 | | |
22580 | | > fitmap #32.4 inMap #18 |
22581 | | |
22582 | | Fit molecule COPI_cyto_J4027_model D (#32.4) to map |
22583 | | W20_J4027_consensus_resampled.mrc (#18) using 4745 atoms |
22584 | | average map value = 0.2737, steps = 64 |
22585 | | shifted from previous position = 0.424 |
22586 | | rotated from previous position = 0.652 degrees |
22587 | | atoms outside contour = 1745, contour level = 0.25259 |
22588 | | |
22589 | | Position of COPI_cyto_J4027_model D (#32.4) relative to |
22590 | | W20_J4027_consensus_resampled.mrc (#18) coordinates: |
22591 | | Matrix rotation and translation |
22592 | | -0.37438641 -0.91150980 -0.17024900 276.33175026 |
22593 | | -0.80075946 0.22523142 0.55502712 164.71539509 |
22594 | | -0.46756723 0.34412311 -0.81422367 240.98992687 |
22595 | | Axis -0.55358575 0.78040778 0.29070004 |
22596 | | Axis point 210.61143312 0.00000000 71.24312694 |
22597 | | Rotation angle (degrees) 169.01867576 |
22598 | | Shift along axis 45.62763928 |
22599 | | |
22600 | | |
22601 | | > fitmap #32.5 inMap #18 |
22602 | | |
22603 | | Fit molecule COPI_cyto_J4027_model E (#32.5) to map |
22604 | | W20_J4027_consensus_resampled.mrc (#18) using 1876 atoms |
22605 | | average map value = 0.3307, steps = 48 |
22606 | | shifted from previous position = 0.367 |
22607 | | rotated from previous position = 3.19 degrees |
22608 | | atoms outside contour = 589, contour level = 0.25259 |
22609 | | |
22610 | | Position of COPI_cyto_J4027_model E (#32.5) relative to |
22611 | | W20_J4027_consensus_resampled.mrc (#18) coordinates: |
22612 | | Matrix rotation and translation |
22613 | | -0.38890629 -0.90726627 -0.16006193 277.40112056 |
22614 | | -0.76465276 0.22097260 0.60537367 157.20851597 |
22615 | | -0.51386581 0.35782543 -0.77968129 242.84222727 |
22616 | | Axis -0.54436333 0.77802149 0.31360984 |
22617 | | Axis point 210.57407631 0.00000000 67.28775591 |
22618 | | Rotation angle (degrees) 166.85747662 |
22619 | | Shift along axis 47.46231881 |
22620 | | |
22621 | | |
22622 | | > fitmap #32.6 inMap #18 |
22623 | | |
22624 | | Fit molecule COPI_cyto_J4027_model F (#32.6) to map |
22625 | | W20_J4027_consensus_resampled.mrc (#18) using 2400 atoms |
22626 | | average map value = 0.1739, steps = 100 |
22627 | | shifted from previous position = 2.11 |
22628 | | rotated from previous position = 17.6 degrees |
22629 | | atoms outside contour = 2400, contour level = 0.25259 |
22630 | | |
22631 | | Position of COPI_cyto_J4027_model F (#32.6) relative to |
22632 | | W20_J4027_consensus_resampled.mrc (#18) coordinates: |
22633 | | Matrix rotation and translation |
22634 | | -0.22469468 -0.97442669 0.00222317 252.72099618 |
22635 | | -0.68062183 0.15857762 0.71526714 144.63194145 |
22636 | | -0.69732793 0.15920358 -0.69884762 265.52538785 |
22637 | | Axis -0.59112239 0.74365656 0.31232874 |
22638 | | Axis point 226.29273798 0.00000000 55.85483264 |
22639 | | Rotation angle (degrees) 151.94327126 |
22640 | | Shift along axis 41.09866330 |
22641 | | |
22642 | | |
22643 | | > ui tool show Matchmaker |
22644 | | |
22645 | | > matchmaker #32.6 to #33 |
22646 | | |
22647 | | Parameters |
22648 | | --- |
22649 | | Chain pairing | bb |
22650 | | Alignment algorithm | Needleman-Wunsch |
22651 | | Similarity matrix | BLOSUM-62 |
22652 | | SS fraction | 0.3 |
22653 | | Gap open (HH/SS/other) | 18/18/6 |
22654 | | Gap extend | 1 |
22655 | | SS matrix | | | H | S | O |
22656 | | ---|---|---|--- |
22657 | | H | 6 | -9 | -6 |
22658 | | S | | 6 | -6 |
22659 | | O | | | 4 |
22660 | | Iteration cutoff | 2 |
22661 | | |
22662 | | Matchmaker COPI_cyto_J4028_model, chain F (#33) with COPI_cyto_J4027_model F, |
22663 | | chain F (#32.6), sequence alignment score = 1592 |
22664 | | RMSD between 299 pruned atom pairs is 0.000 angstroms; (across all 299 pairs: |
22665 | | 0.000) |
22666 | | |
22667 | | |
22668 | | > hide #!32 models |
22669 | | |
22670 | | > show #!32 models |
22671 | | |
22672 | | > hide #!32 models |
22673 | | |
22674 | | > hide #!18 models |
22675 | | |
22676 | | > show #!20 models |
22677 | | |
22678 | | > color #20 darkgrey models |
22679 | | |
22680 | | > color #20 #a9a9a9c2 models |
22681 | | |
22682 | | > show #!33 models |
22683 | | |
22684 | | > split #33 chains |
22685 | | |
22686 | | Split COPI_cyto_J4028_model (#33) into 13 models |
22687 | | Chain information for COPI_cyto_J4028_model A #33.1 |
22688 | | --- |
22689 | | Chain | Description |
22690 | | A | No description available |
22691 | | |
22692 | | Chain information for COPI_cyto_J4028_model B #33.2 |
22693 | | --- |
22694 | | Chain | Description |
22695 | | B | No description available |
22696 | | |
22697 | | Chain information for COPI_cyto_J4028_model C #33.3 |
22698 | | --- |
22699 | | Chain | Description |
22700 | | C | No description available |
22701 | | |
22702 | | Chain information for COPI_cyto_J4028_model D #33.4 |
22703 | | --- |
22704 | | Chain | Description |
22705 | | D | No description available |
22706 | | |
22707 | | Chain information for COPI_cyto_J4028_model E #33.5 |
22708 | | --- |
22709 | | Chain | Description |
22710 | | E | No description available |
22711 | | |
22712 | | Chain information for COPI_cyto_J4028_model F #33.6 |
22713 | | --- |
22714 | | Chain | Description |
22715 | | F | No description available |
22716 | | |
22717 | | Chain information for COPI_cyto_J4028_model G #33.7 |
22718 | | --- |
22719 | | Chain | Description |
22720 | | G | No description available |
22721 | | |
22722 | | Chain information for COPI_cyto_J4028_model H #33.8 |
22723 | | --- |
22724 | | Chain | Description |
22725 | | H | No description available |
22726 | | |
22727 | | Chain information for COPI_cyto_J4028_model I #33.9 |
22728 | | --- |
22729 | | Chain | Description |
22730 | | I | No description available |
22731 | | |
22732 | | Chain information for COPI_cyto_J4028_model J #33.10 |
22733 | | --- |
22734 | | Chain | Description |
22735 | | J | No description available |
22736 | | |
22737 | | Chain information for COPI_cyto_J4028_model K #33.11 |
22738 | | --- |
22739 | | Chain | Description |
22740 | | K | No description available |
22741 | | |
22742 | | Chain information for COPI_cyto_J4028_model L #33.12 |
22743 | | --- |
22744 | | Chain | Description |
22745 | | L | No description available |
22746 | | |
22747 | | Chain information for COPI_cyto_J4028_model M #33.13 |
22748 | | --- |
22749 | | Chain | Description |
22750 | | M | No description available |
22751 | | |
22752 | | |
22753 | | > fitmap #33.1 inMap #20 |
22754 | | |
22755 | | Fit molecule COPI_cyto_J4028_model A (#33.1) to map |
22756 | | W20_J4028_consensus_resampled.mrc (#20) using 2590 atoms |
22757 | | average map value = 0.3839, steps = 84 |
22758 | | shifted from previous position = 1.3 |
22759 | | rotated from previous position = 3.69 degrees |
22760 | | atoms outside contour = 606, contour level = 0.25474 |
22761 | | |
22762 | | Position of COPI_cyto_J4028_model A (#33.1) relative to |
22763 | | W20_J4028_consensus_resampled.mrc (#20) coordinates: |
22764 | | Matrix rotation and translation |
22765 | | -0.42505705 -0.88684737 -0.18118571 281.77112295 |
22766 | | -0.78040543 0.25764379 0.56972543 159.62921913 |
22767 | | -0.45857812 0.38356412 -0.80161381 237.17247148 |
22768 | | Axis -0.53095306 0.79115444 0.30358443 |
22769 | | Axis point 206.86596376 0.00000000 70.78540234 |
22770 | | Rotation angle (degrees) 169.90338089 |
22771 | | Shift along axis 48.68599577 |
22772 | | |
22773 | | |
22774 | | > fitmap #33.2 inMap #20 |
22775 | | |
22776 | | Fit molecule COPI_cyto_J4028_model B (#33.2) to map |
22777 | | W20_J4028_consensus_resampled.mrc (#20) using 2426 atoms |
22778 | | average map value = 0.3703, steps = 76 |
22779 | | shifted from previous position = 2 |
22780 | | rotated from previous position = 3.77 degrees |
22781 | | atoms outside contour = 594, contour level = 0.25474 |
22782 | | |
22783 | | Position of COPI_cyto_J4028_model B (#33.2) relative to |
22784 | | W20_J4028_consensus_resampled.mrc (#20) coordinates: |
22785 | | Matrix rotation and translation |
22786 | | -0.42163847 -0.88604192 -0.19274527 282.36522569 |
22787 | | -0.78360399 0.24908325 0.56914176 161.05650807 |
22788 | | -0.45627383 0.39100801 -0.79932906 236.28096107 |
22789 | | Axis -0.53305638 0.78859614 0.30654042 |
22790 | | Axis point 207.42054850 0.00000000 70.38397992 |
22791 | | Rotation angle (degrees) 170.38149718 |
22792 | | Shift along axis 48.92162012 |
22793 | | |
22794 | | |
22795 | | > fitmap #33.3 inMap #20 |
22796 | | |
22797 | | Fit molecule COPI_cyto_J4028_model C (#33.3) to map |
22798 | | W20_J4028_consensus_resampled.mrc (#20) using 1612 atoms |
22799 | | average map value = 0.2936, steps = 120 |
22800 | | shifted from previous position = 1.59 |
22801 | | rotated from previous position = 5.86 degrees |
22802 | | atoms outside contour = 556, contour level = 0.25474 |
22803 | | |
22804 | | Position of COPI_cyto_J4028_model C (#33.3) relative to |
22805 | | W20_J4028_consensus_resampled.mrc (#20) coordinates: |
22806 | | Matrix rotation and translation |
22807 | | -0.38874794 -0.91700982 -0.08926390 273.70817090 |
22808 | | -0.74150605 0.25389180 0.62105374 153.76404568 |
22809 | | -0.54684900 0.30762308 -0.77866824 247.74574422 |
22810 | | Axis -0.53877892 0.78657655 0.30168628 |
22811 | | Axis point 211.55093832 0.00000000 66.06733162 |
22812 | | Rotation angle (degrees) 163.08987268 |
22813 | | Shift along axis 48.22049311 |
22814 | | |
22815 | | |
22816 | | > fitmap #33.4 inMap #20 |
22817 | | |
22818 | | Fit molecule COPI_cyto_J4028_model D (#33.4) to map |
22819 | | W20_J4028_consensus_resampled.mrc (#20) using 4745 atoms |
22820 | | average map value = 0.2743, steps = 52 |
22821 | | shifted from previous position = 0.325 |
22822 | | rotated from previous position = 2.63 degrees |
22823 | | atoms outside contour = 1777, contour level = 0.25474 |
22824 | | |
22825 | | Position of COPI_cyto_J4028_model D (#33.4) relative to |
22826 | | W20_J4028_consensus_resampled.mrc (#20) coordinates: |
22827 | | Matrix rotation and translation |
22828 | | -0.35425466 -0.92574203 -0.13230776 272.21729531 |
22829 | | -0.80879167 0.23228423 0.54027779 166.54524500 |
22830 | | -0.46942484 0.29840534 -0.83102021 247.09025195 |
22831 | | Axis -0.56108841 0.78203404 0.27129791 |
22832 | | Axis point 212.23977312 0.00000000 73.93457988 |
22833 | | Rotation angle (degrees) 167.55287093 |
22834 | | Shift along axis 44.54114989 |
22835 | | |
22836 | | |
22837 | | > fitmap #33.5 inMap #20 |
22838 | | |
22839 | | Fit molecule COPI_cyto_J4028_model E (#33.5) to map |
22840 | | W20_J4028_consensus_resampled.mrc (#20) using 1876 atoms |
22841 | | average map value = 0.3337, steps = 68 |
22842 | | shifted from previous position = 0.86 |
22843 | | rotated from previous position = 3.26 degrees |
22844 | | atoms outside contour = 607, contour level = 0.25474 |
22845 | | |
22846 | | Position of COPI_cyto_J4028_model E (#33.5) relative to |
22847 | | W20_J4028_consensus_resampled.mrc (#20) coordinates: |
22848 | | Matrix rotation and translation |
22849 | | -0.40812036 -0.89487089 -0.18067614 280.82990976 |
22850 | | -0.77162408 0.23236180 0.59211848 158.56716868 |
22851 | | -0.48788736 0.38106967 -0.78533550 239.27651010 |
22852 | | Axis -0.53761002 0.78256694 0.31394976 |
22853 | | Axis point 209.07381918 0.00000000 68.40687366 |
22854 | | Rotation angle (degrees) 168.68024136 |
22855 | | Shift along axis 48.23325430 |
22856 | | |
22857 | | |
22858 | | > fitmap #33.10 inMap #20 |
22859 | | |
22860 | | Fit molecule COPI_cyto_J4028_model J (#33.10) to map |
22861 | | W20_J4028_consensus_resampled.mrc (#20) using 1112 atoms |
22862 | | average map value = 0.2746, steps = 88 |
22863 | | shifted from previous position = 1.18 |
22864 | | rotated from previous position = 6.07 degrees |
22865 | | atoms outside contour = 487, contour level = 0.25474 |
22866 | | |
22867 | | Position of COPI_cyto_J4028_model J (#33.10) relative to |
22868 | | W20_J4028_consensus_resampled.mrc (#20) coordinates: |
22869 | | Matrix rotation and translation |
22870 | | -0.37976892 -0.92311743 -0.06024771 266.93454127 |
22871 | | -0.77672422 0.28281318 0.56277545 158.41114514 |
22872 | | -0.50246898 0.26052048 -0.82441132 252.69246283 |
22873 | | Axis -0.54431763 0.79637675 0.26363308 |
22874 | | Axis point 208.95699781 0.00000000 74.09614523 |
22875 | | Rotation angle (degrees) 163.88025316 |
22876 | | Shift along axis 47.47586703 |
22877 | | |
22878 | | |
22879 | | > fitmap #33.12 inMap #20 |
22880 | | |
22881 | | Fit molecule COPI_cyto_J4028_model L (#33.12) to map |
22882 | | W20_J4028_consensus_resampled.mrc (#20) using 1420 atoms |
22883 | | average map value = 0.2305, steps = 152 |
22884 | | shifted from previous position = 15.6 |
22885 | | rotated from previous position = 25.9 degrees |
22886 | | atoms outside contour = 812, contour level = 0.25474 |
22887 | | |
22888 | | Position of COPI_cyto_J4028_model L (#33.12) relative to |
22889 | | W20_J4028_consensus_resampled.mrc (#20) coordinates: |
22890 | | Matrix rotation and translation |
22891 | | -0.06107414 -0.89318761 -0.44551751 275.05032468 |
22892 | | -0.79982244 -0.22322988 0.55718265 197.31395144 |
22893 | | -0.59712146 0.39036435 -0.70075791 230.69793847 |
22894 | | Axis -0.68372085 0.62136335 0.38266615 |
22895 | | Axis point 0.00000000 233.57747065 184.11991319 |
22896 | | Rotation angle (degrees) 172.99286255 |
22897 | | Shift along axis 22.82630694 |
22898 | | |
22899 | | |
22900 | | > select add #33.12 |
22901 | | |
22902 | | 1420 atoms, 1441 bonds, 177 residues, 1 model selected |
22903 | | |
22904 | | > view matrix models |
22905 | | > #33.12,-0.71047,-0.45523,-0.53665,342.29,-0.62968,0.75173,0.19595,130.11,0.31422,0.47714,-0.82074,104.42 |
22906 | | |
22907 | | > view matrix models |
22908 | | > #33.12,-0.87976,-0.194,-0.43403,328.35,-0.31813,0.91866,0.23421,62.94,0.35329,0.34413,-0.86992,118.06 |
22909 | | |
22910 | | > fitmap #33.12 inMap #20 |
22911 | | |
22912 | | Fit molecule COPI_cyto_J4028_model L (#33.12) to map |
22913 | | W20_J4028_consensus_resampled.mrc (#20) using 1420 atoms |
22914 | | average map value = 0.219, steps = 148 |
22915 | | shifted from previous position = 4.64 |
22916 | | rotated from previous position = 34.8 degrees |
22917 | | atoms outside contour = 813, contour level = 0.25474 |
22918 | | |
22919 | | Position of COPI_cyto_J4028_model L (#33.12) relative to |
22920 | | W20_J4028_consensus_resampled.mrc (#20) coordinates: |
22921 | | Matrix rotation and translation |
22922 | | -0.65509662 -0.64625356 -0.39141380 328.46645180 |
22923 | | -0.75535360 0.57185725 0.32003163 147.76949721 |
22924 | | 0.01701124 0.50530746 -0.86277170 152.27605950 |
22925 | | Axis 0.40140909 -0.88487271 -0.23637052 |
22926 | | Axis point 188.23063104 0.00000000 79.03730027 |
22927 | | Rotation angle (degrees) 166.65689588 |
22928 | | Shift along axis -34.90134836 |
22929 | | |
22930 | | |
22931 | | > ui mousemode right "rotate selected models" |
22932 | | |
22933 | | > view matrix models |
22934 | | > #33.12,-0.03029,-0.7598,0.64945,102.21,-0.99925,0.0072683,-0.038101,287,0.024229,-0.65012,-0.75945,246.16 |
22935 | | |
22936 | | > fitmap #33.12 inMap #20 |
22937 | | |
22938 | | Fit molecule COPI_cyto_J4028_model L (#33.12) to map |
22939 | | W20_J4028_consensus_resampled.mrc (#20) using 1420 atoms |
22940 | | average map value = 0.2032, steps = 160 |
22941 | | shifted from previous position = 13.1 |
22942 | | rotated from previous position = 20.8 degrees |
22943 | | atoms outside contour = 909, contour level = 0.25474 |
22944 | | |
22945 | | Position of COPI_cyto_J4028_model L (#33.12) relative to |
22946 | | W20_J4028_consensus_resampled.mrc (#20) coordinates: |
22947 | | Matrix rotation and translation |
22948 | | 0.16998318 -0.52866209 0.83163822 33.05834902 |
22949 | | -0.98535039 -0.07936651 0.15094897 271.66299549 |
22950 | | -0.01379677 -0.84511382 -0.53440836 228.58308709 |
22951 | | Axis -0.71969674 0.61086190 -0.32997627 |
22952 | | Axis point 0.00000000 219.83009454 42.24482979 |
22953 | | Rotation angle (degrees) 136.21122723 |
22954 | | Shift along axis 66.72959442 |
22955 | | |
22956 | | |
22957 | | > ui mousemode right "translate selected models" |
22958 | | |
22959 | | > view matrix models |
22960 | | > #33.12,0.16998,-0.52866,0.83164,40.813,-0.98535,-0.079367,0.15095,266.5,-0.013797,-0.84511,-0.53441,239.35 |
22961 | | |
22962 | | > ui mousemode right "rotate selected models" |
22963 | | |
22964 | | > view matrix models |
22965 | | > #33.12,-0.43029,-0.87383,0.22644,246.31,-0.87355,0.46631,0.13952,199.93,-0.22751,-0.13778,-0.96398,263.72 |
22966 | | |
22967 | | > ui mousemode right "translate selected models" |
22968 | | |
22969 | | > view matrix models |
22970 | | > #33.12,-0.43029,-0.87383,0.22644,240.63,-0.87355,0.46631,0.13952,199.48,-0.22751,-0.13778,-0.96398,257.34 |
22971 | | |
22972 | | > fitmap #33.12 inMap #20 |
22973 | | |
22974 | | Fit molecule COPI_cyto_J4028_model L (#33.12) to map |
22975 | | W20_J4028_consensus_resampled.mrc (#20) using 1420 atoms |
22976 | | average map value = 0.259, steps = 136 |
22977 | | shifted from previous position = 2.22 |
22978 | | rotated from previous position = 19 degrees |
22979 | | atoms outside contour = 643, contour level = 0.25474 |
22980 | | |
22981 | | Position of COPI_cyto_J4028_model L (#33.12) relative to |
22982 | | W20_J4028_consensus_resampled.mrc (#20) coordinates: |
22983 | | Matrix rotation and translation |
22984 | | -0.11376158 -0.96773135 0.22484296 202.04835591 |
22985 | | -0.96542922 0.16110229 0.20492070 231.97393999 |
22986 | | -0.23453090 -0.19375786 -0.95260336 262.84035133 |
22987 | | Axis -0.65544634 0.75523227 0.00378481 |
22988 | | Axis point 228.77213631 0.00000000 107.04713970 |
22989 | | Rotation angle (degrees) 162.29431769 |
22990 | | Shift along axis 43.75715061 |
22991 | | |
22992 | | |
22993 | | > select subtract #33.12 |
22994 | | |
22995 | | Nothing selected |
22996 | | |
22997 | | > fitmap #33.13 inMap #20 |
22998 | | |
22999 | | Fit molecule COPI_cyto_J4028_model M (#33.13) to map |
23000 | | W20_J4028_consensus_resampled.mrc (#20) using 4552 atoms |
23001 | | average map value = 0.2597, steps = 164 |
23002 | | shifted from previous position = 11.9 |
23003 | | rotated from previous position = 21.2 degrees |
23004 | | atoms outside contour = 2429, contour level = 0.25474 |
23005 | | |
23006 | | Position of COPI_cyto_J4028_model M (#33.13) relative to |
23007 | | W20_J4028_consensus_resampled.mrc (#20) coordinates: |
23008 | | Matrix rotation and translation |
23009 | | -0.41064746 -0.90764367 0.08690026 262.06407158 |
23010 | | -0.84733447 0.41507760 0.33126258 174.12202496 |
23011 | | -0.33673873 0.06239856 -0.93952832 256.95292530 |
23012 | | Axis -0.53201816 0.83828111 0.11933760 |
23013 | | Axis point 205.62822196 0.00000000 94.49206914 |
23014 | | Rotation angle (degrees) 165.36365713 |
23015 | | Shift along axis 37.20450278 |
23016 | | |
23017 | | |
23018 | | > show #!32 models |
23019 | | |
23020 | | > hide #!33 models |
23021 | | |
23022 | | > hide #!32 models |
23023 | | |
23024 | | > show #!33 models |
23025 | | |
23026 | | > hide #!33 models |
23027 | | |
23028 | | > split #34 chains |
23029 | | |
23030 | | Split COPI_cyto_J3988_model (#34) into 13 models |
23031 | | Chain information for COPI_cyto_J3988_model A #34.1 |
23032 | | --- |
23033 | | Chain | Description |
23034 | | A | No description available |
23035 | | |
23036 | | Chain information for COPI_cyto_J3988_model B #34.2 |
23037 | | --- |
23038 | | Chain | Description |
23039 | | B | No description available |
23040 | | |
23041 | | Chain information for COPI_cyto_J3988_model C #34.3 |
23042 | | --- |
23043 | | Chain | Description |
23044 | | C | No description available |
23045 | | |
23046 | | Chain information for COPI_cyto_J3988_model D #34.4 |
23047 | | --- |
23048 | | Chain | Description |
23049 | | D | No description available |
23050 | | |
23051 | | Chain information for COPI_cyto_J3988_model E #34.5 |
23052 | | --- |
23053 | | Chain | Description |
23054 | | E | No description available |
23055 | | |
23056 | | Chain information for COPI_cyto_J3988_model F #34.6 |
23057 | | --- |
23058 | | Chain | Description |
23059 | | F | No description available |
23060 | | |
23061 | | Chain information for COPI_cyto_J3988_model G #34.7 |
23062 | | --- |
23063 | | Chain | Description |
23064 | | G | No description available |
23065 | | |
23066 | | Chain information for COPI_cyto_J3988_model H #34.8 |
23067 | | --- |
23068 | | Chain | Description |
23069 | | H | No description available |
23070 | | |
23071 | | Chain information for COPI_cyto_J3988_model I #34.9 |
23072 | | --- |
23073 | | Chain | Description |
23074 | | I | No description available |
23075 | | |
23076 | | Chain information for COPI_cyto_J3988_model J #34.10 |
23077 | | --- |
23078 | | Chain | Description |
23079 | | J | No description available |
23080 | | |
23081 | | Chain information for COPI_cyto_J3988_model K #34.11 |
23082 | | --- |
23083 | | Chain | Description |
23084 | | K | No description available |
23085 | | |
23086 | | Chain information for COPI_cyto_J3988_model L #34.12 |
23087 | | --- |
23088 | | Chain | Description |
23089 | | L | No description available |
23090 | | |
23091 | | Chain information for COPI_cyto_J3988_model M #34.13 |
23092 | | --- |
23093 | | Chain | Description |
23094 | | M | No description available |
23095 | | |
23096 | | |
23097 | | > show #34.1 models |
23098 | | |
23099 | | > show #34.2 models |
23100 | | |
23101 | | > show #34.3 models |
23102 | | |
23103 | | > show #!34.4 models |
23104 | | |
23105 | | > show #34.5 models |
23106 | | |
23107 | | > show #34.6 models |
23108 | | |
23109 | | > show #34.7 models |
23110 | | |
23111 | | > show #34.8 models |
23112 | | |
23113 | | > show #34.9 models |
23114 | | |
23115 | | > show #34.10 models |
23116 | | |
23117 | | > show #34.11 models |
23118 | | |
23119 | | > show #34.12 models |
23120 | | |
23121 | | > show #34.13 models |
23122 | | |
23123 | | > hide #!20 models |
23124 | | |
23125 | | > show #!22 models |
23126 | | |
23127 | | > color #22 darkgrey models |
23128 | | |
23129 | | > color #22 #a9a9a9bf models |
23130 | | |
23131 | | > fitmap #34.1 inMap #22 |
23132 | | |
23133 | | Fit molecule COPI_cyto_J3988_model A (#34.1) to map |
23134 | | W20_J3988_consensus_resampled.mrc (#22) using 2590 atoms |
23135 | | average map value = 0.3688, steps = 88 |
23136 | | shifted from previous position = 1.25 |
23137 | | rotated from previous position = 3.5 degrees |
23138 | | atoms outside contour = 749, contour level = 0.28583 |
23139 | | |
23140 | | Position of COPI_cyto_J3988_model A (#34.1) relative to |
23141 | | W20_J3988_consensus_resampled.mrc (#22) coordinates: |
23142 | | Matrix rotation and translation |
23143 | | -0.41785370 -0.88839960 -0.19011695 282.28257009 |
23144 | | -0.78880679 0.25093956 0.56108217 162.05511406 |
23145 | | -0.45075731 0.38441580 -0.80563165 235.85823021 |
23146 | | Axis -0.53495212 0.78922836 0.30157060 |
23147 | | Axis point 207.72235198 0.00000000 70.74799729 |
23148 | | Rotation angle (degrees) 170.49558915 |
23149 | | Shift along axis 48.01874106 |
23150 | | |
23151 | | |
23152 | | > fitmap #34.2 inMap #22 |
23153 | | |
23154 | | Fit molecule COPI_cyto_J3988_model B (#34.2) to map |
23155 | | W20_J3988_consensus_resampled.mrc (#22) using 2426 atoms |
23156 | | average map value = 0.3348, steps = 104 |
23157 | | shifted from previous position = 1.68 |
23158 | | rotated from previous position = 3.88 degrees |
23159 | | atoms outside contour = 828, contour level = 0.28583 |
23160 | | |
23161 | | Position of COPI_cyto_J3988_model B (#34.2) relative to |
23162 | | W20_J3988_consensus_resampled.mrc (#22) coordinates: |
23163 | | Matrix rotation and translation |
23164 | | -0.42101840 -0.88538396 -0.19707555 283.10669504 |
23165 | | -0.78443773 0.24632045 0.56919566 160.85163308 |
23166 | | -0.45541296 0.39423534 -0.79823402 235.48770247 |
23167 | | Axis -0.53352603 0.78777711 0.30782658 |
23168 | | Axis point 207.58242660 0.00000000 70.13942680 |
23169 | | Rotation angle (degrees) 170.56282406 |
23170 | | Shift along axis 48.15981832 |
23171 | | |
23172 | | |
23173 | | > fitmap #34.3 inMap #22 |
23174 | | |
23175 | | Fit molecule COPI_cyto_J3988_model C (#34.3) to map |
23176 | | W20_J3988_consensus_resampled.mrc (#22) using 1612 atoms |
23177 | | average map value = 0.3235, steps = 80 |
23178 | | shifted from previous position = 1.01 |
23179 | | rotated from previous position = 1.39 degrees |
23180 | | atoms outside contour = 647, contour level = 0.28583 |
23181 | | |
23182 | | Position of COPI_cyto_J3988_model C (#34.3) relative to |
23183 | | W20_J3988_consensus_resampled.mrc (#22) coordinates: |
23184 | | Matrix rotation and translation |
23185 | | -0.38953007 -0.90460759 -0.17306487 278.08945587 |
23186 | | -0.79075902 0.23214543 0.56639976 162.53472037 |
23187 | | -0.47219330 0.35748234 -0.80575423 240.06559363 |
23188 | | Axis -0.54660128 0.78262495 0.29786781 |
23189 | | Axis point 209.73775516 0.00000000 70.39505904 |
23190 | | Rotation angle (degrees) 168.98267188 |
23191 | | Shift along axis 46.70748572 |
23192 | | |
23193 | | |
23194 | | > fitmap #34.4 inMap #22 |
23195 | | |
23196 | | Fit molecule COPI_cyto_J3988_model D (#34.4) to map |
23197 | | W20_J3988_consensus_resampled.mrc (#22) using 4745 atoms |
23198 | | average map value = 0.341, steps = 72 |
23199 | | shifted from previous position = 0.451 |
23200 | | rotated from previous position = 2.53 degrees |
23201 | | atoms outside contour = 1760, contour level = 0.28583 |
23202 | | |
23203 | | Position of COPI_cyto_J3988_model D (#34.4) relative to |
23204 | | W20_J3988_consensus_resampled.mrc (#22) coordinates: |
23205 | | Matrix rotation and translation |
23206 | | -0.35609850 -0.92473928 -0.13435451 272.54654270 |
23207 | | -0.80894027 0.23309315 0.53970662 166.76836324 |
23208 | | -0.46777080 0.30087349 -0.83106296 247.02887626 |
23209 | | Axis -0.56042334 0.78236325 0.27172306 |
23210 | | Axis point 212.17904341 0.00000000 74.04958983 |
23211 | | Rotation angle (degrees) 167.69692699 |
23212 | | Shift along axis 44.85543655 |
23213 | | |
23214 | | |
23215 | | > fitmap #34.5 inMap #22 |
23216 | | |
23217 | | Fit molecule COPI_cyto_J3988_model E (#34.5) to map |
23218 | | W20_J3988_consensus_resampled.mrc (#22) using 1876 atoms |
23219 | | average map value = 0.3399, steps = 68 |
23220 | | shifted from previous position = 0.902 |
23221 | | rotated from previous position = 1.88 degrees |
23222 | | atoms outside contour = 644, contour level = 0.28583 |
23223 | | |
23224 | | Position of COPI_cyto_J3988_model E (#34.5) relative to |
23225 | | W20_J3988_consensus_resampled.mrc (#22) coordinates: |
23226 | | Matrix rotation and translation |
23227 | | -0.38559316 -0.90595853 -0.17480580 278.32647192 |
23228 | | -0.80563868 0.23824013 0.54239099 166.10780051 |
23229 | | -0.44973798 0.34997256 -0.82173899 240.13441550 |
23230 | | Axis -0.54937100 0.78495483 0.28642174 |
23231 | | Axis point 210.04384962 0.00000000 72.68833745 |
23232 | | Rotation angle (degrees) 169.91400249 |
23233 | | Shift along axis 46.26234424 |
23234 | | |
23235 | | |
23236 | | > fitmap #34.10 inMap #22 |
23237 | | |
23238 | | Fit molecule COPI_cyto_J3988_model J (#34.10) to map |
23239 | | W20_J3988_consensus_resampled.mrc (#22) using 1112 atoms |
23240 | | average map value = 0.314, steps = 56 |
23241 | | shifted from previous position = 0.967 |
23242 | | rotated from previous position = 5.85 degrees |
23243 | | atoms outside contour = 470, contour level = 0.28583 |
23244 | | |
23245 | | Position of COPI_cyto_J3988_model J (#34.10) relative to |
23246 | | W20_J3988_consensus_resampled.mrc (#22) coordinates: |
23247 | | Matrix rotation and translation |
23248 | | -0.37577208 -0.92422463 -0.06785421 266.88773036 |
23249 | | -0.79027325 0.28134549 0.54434632 161.90561717 |
23250 | | -0.48400780 0.25817351 -0.83611178 252.25402649 |
23251 | | Axis -0.54768370 0.79644372 0.25635904 |
23252 | | Axis point 209.14918816 0.00000000 75.62770199 |
23253 | | Rotation angle (degrees) 164.85531677 |
23254 | | Shift along axis 47.44625196 |
23255 | | |
23256 | | |
23257 | | > fitmap #34.12 inMap #22 |
23258 | | |
23259 | | Fit molecule COPI_cyto_J3988_model L (#34.12) to map |
23260 | | W20_J3988_consensus_resampled.mrc (#22) using 1420 atoms |
23261 | | average map value = 0.2477, steps = 128 |
23262 | | shifted from previous position = 15.9 |
23263 | | rotated from previous position = 23.9 degrees |
23264 | | atoms outside contour = 825, contour level = 0.28583 |
23265 | | |
23266 | | Position of COPI_cyto_J3988_model L (#34.12) relative to |
23267 | | W20_J3988_consensus_resampled.mrc (#22) coordinates: |
23268 | | Matrix rotation and translation |
23269 | | -0.13744140 -0.87706877 -0.46028280 286.39333689 |
23270 | | -0.79755718 -0.17757218 0.57651599 190.01595705 |
23271 | | -0.58737759 0.44633901 -0.67510671 220.17263411 |
23272 | | Axis -0.65564558 0.64012190 0.40046576 |
23273 | | Axis point 0.00000000 242.83430967 190.87489306 |
23274 | | Rotation angle (degrees) 174.30264049 |
23275 | | Shift along axis 22.03245138 |
23276 | | |
23277 | | |
23278 | | > ui mousemode right "rotate selected models" |
23279 | | |
23280 | | > select add #34.12 |
23281 | | |
23282 | | 1420 atoms, 1441 bonds, 177 residues, 1 model selected |
23283 | | |
23284 | | > view matrix models |
23285 | | > #34.12,0.094052,-0.95592,0.27815,157.02,-0.86993,0.056954,0.48988,190.57,-0.48413,-0.28805,-0.82622,295.38 |
23286 | | |
23287 | | > fitmap #34.12 inMap #22 |
23288 | | |
23289 | | Fit molecule COPI_cyto_J3988_model L (#34.12) to map |
23290 | | W20_J3988_consensus_resampled.mrc (#22) using 1420 atoms |
23291 | | average map value = 0.265, steps = 180 |
23292 | | shifted from previous position = 7.48 |
23293 | | rotated from previous position = 16.7 degrees |
23294 | | atoms outside contour = 709, contour level = 0.28583 |
23295 | | |
23296 | | Position of COPI_cyto_J3988_model L (#34.12) relative to |
23297 | | W20_J3988_consensus_resampled.mrc (#22) coordinates: |
23298 | | Matrix rotation and translation |
23299 | | -0.10354097 -0.97915690 0.17473139 207.46054459 |
23300 | | -0.94372455 0.15219348 0.29363435 218.75648454 |
23301 | | -0.31410708 -0.13449511 -0.93981265 267.23328820 |
23302 | | Axis -0.65787226 0.75115891 0.05444605 |
23303 | | Axis point 229.01761440 0.00000000 99.48866389 |
23304 | | Rotation angle (degrees) 161.01082491 |
23305 | | Shift along axis 42.38814162 |
23306 | | |
23307 | | |
23308 | | > select subtract #34.12 |
23309 | | |
23310 | | Nothing selected |
23311 | | |
23312 | | > fitmap #34.13 inMap #22 |
23313 | | |
23314 | | Fit molecule COPI_cyto_J3988_model M (#34.13) to map |
23315 | | W20_J3988_consensus_resampled.mrc (#22) using 4552 atoms |
23316 | | average map value = 0.2757, steps = 176 |
23317 | | shifted from previous position = 11.7 |
23318 | | rotated from previous position = 23.1 degrees |
23319 | | atoms outside contour = 2512, contour level = 0.28583 |
23320 | | |
23321 | | Position of COPI_cyto_J3988_model M (#34.13) relative to |
23322 | | W20_J3988_consensus_resampled.mrc (#22) coordinates: |
23323 | | Matrix rotation and translation |
23324 | | -0.23360378 -0.97224105 0.01329047 246.50433978 |
23325 | | -0.93813795 0.22896058 0.25975806 213.83868979 |
23326 | | -0.25559044 0.04821217 -0.96558227 246.39586728 |
23327 | | Axis -0.61528240 0.78204162 0.09918904 |
23328 | | Axis point 220.79513963 0.00000000 94.62595818 |
23329 | | Rotation angle (degrees) 170.10113474 |
23330 | | Shift along axis 40.00074310 |
23331 | | |
23332 | | |
23333 | | > save "/Users/becca/Desktop/Postdoc/COPI notebooks and |
23334 | | > resources/Processing/W20_MM_cyto_COPI/20250707_W20_COPI_cyto_alignments.cxs" |
23335 | | |
23336 | | > open |
23337 | | > /Users/becca/Desktop/Postdoc/Phenix/W20_MM_cyto_COPI/ResolveCryoEM_29/denmod_map.ccp4 |
23338 | | |
23339 | | Opened denmod_map.ccp4 as #35, grid size 99,98,133, pixel 1.19, shown at level |
23340 | | 0.318, step 1, values float32 |
23341 | | |
23342 | | > open |
23343 | | > /Users/becca/Desktop/Postdoc/Phenix/W20_MM_cyto_COPI/ResolveCryoEM_30/denmod_map.ccp4 |
23344 | | |
23345 | | Opened denmod_map.ccp4 as #36, grid size 97,98,137, pixel 1.19, shown at level |
23346 | | 0.404, step 1, values float32 |
23347 | | |
23348 | | > hide #!34 models |
23349 | | |
23350 | | > hide #!22 models |
23351 | | |
23352 | | > hide #!36 models |
23353 | | |
23354 | | > volume #35 level 0.105 |
23355 | | |
23356 | | > show #!34 models |
23357 | | |
23358 | | > hide #!35 models |
23359 | | |
23360 | | > show #!36 models |
23361 | | |
23362 | | > volume #36 level 0.1681 |
23363 | | |
23364 | | > hide #!36 models |
23365 | | |
23366 | | > close #35-36 |
23367 | | |
23368 | | > open |
23369 | | > /Users/becca/Desktop/Postdoc/Phenix/W20_MM_cyto_COPI/ResolveCryoEM_29/denmod_map.ccp4 |
23370 | | |
23371 | | Opened denmod_map.ccp4 as #35, grid size 99,98,133, pixel 1.19, shown at level |
23372 | | 0.318, step 1, values float32 |
23373 | | |
23374 | | > close #35 |
23375 | | |
23376 | | > show #!47 models |
23377 | | |
23378 | | > hide #!47 models |
23379 | | |
23380 | | > close #56#47-55,57 |
23381 | | |
23382 | | > show #!94 models |
23383 | | |
23384 | | > hide #!94 models |
23385 | | |
23386 | | > close #71-72,76,78-80,83,86-88#58-70,73-75,77,81-82,84-85,89-95 |
23387 | | |
23388 | | > open "/Users/becca/Desktop/Postdoc/COPI notebooks and resources/Structure |
23389 | | > files/COPI_soluble_job155_dhelix.pdb" |
23390 | | |
23391 | | Chain information for COPI_soluble_job155_dhelix.pdb #35 |
23392 | | --- |
23393 | | Chain | Description |
23394 | | D | No description available |
23395 | | E | No description available |
23396 | | F | No description available |
23397 | | G | No description available |
23398 | | H | No description available |
23399 | | I | No description available |
23400 | | J | No description available |
23401 | | K | No description available |
23402 | | L | No description available |
23403 | | M | No description available |
23404 | | N | No description available |
23405 | | O | No description available |
23406 | | P | No description available |
23407 | | Q | No description available |
23408 | | |
23409 | | |
23410 | | > open "/Users/becca/Desktop/Postdoc/COPI notebooks and resources/Structure |
23411 | | > files/COPI_soluble_job160_dhelix.pdb" |
23412 | | |
23413 | | Chain information for COPI_soluble_job160_dhelix.pdb #36 |
23414 | | --- |
23415 | | Chain | Description |
23416 | | D | No description available |
23417 | | E | No description available |
23418 | | F | No description available |
23419 | | G | No description available |
23420 | | H | No description available |
23421 | | I | No description available |
23422 | | J | No description available |
23423 | | K | No description available |
23424 | | L | No description available |
23425 | | M | No description available |
23426 | | N | No description available |
23427 | | O | No description available |
23428 | | P | No description available |
23429 | | Q | No description available |
23430 | | |
23431 | | |
23432 | | > ui tool show Matchmaker |
23433 | | |
23434 | | > matchmaker #!35-36 to #34.4 |
23435 | | |
23436 | | Parameters |
23437 | | --- |
23438 | | Chain pairing | bb |
23439 | | Alignment algorithm | Needleman-Wunsch |
23440 | | Similarity matrix | BLOSUM-62 |
23441 | | SS fraction | 0.3 |
23442 | | Gap open (HH/SS/other) | 18/18/6 |
23443 | | Gap extend | 1 |
23444 | | SS matrix | | | H | S | O |
23445 | | ---|---|---|--- |
23446 | | H | 6 | -9 | -6 |
23447 | | S | | 6 | -6 |
23448 | | O | | | 4 |
23449 | | Iteration cutoff | 2 |
23450 | | |
23451 | | Matchmaker COPI_cyto_J3988_model D, chain D (#34.4) with |
23452 | | COPI_soluble_job155_dhelix.pdb, chain I (#35), sequence alignment score = 1535 |
23453 | | RMSD between 291 pruned atom pairs is 0.541 angstroms; (across all 305 pairs: |
23454 | | 0.988) |
23455 | | |
23456 | | Matchmaker COPI_cyto_J3988_model D, chain D (#34.4) with |
23457 | | COPI_soluble_job160_dhelix.pdb, chain I (#36), sequence alignment score = 1538 |
23458 | | RMSD between 291 pruned atom pairs is 0.541 angstroms; (across all 305 pairs: |
23459 | | 0.988) |
23460 | | |
23461 | | |
23462 | | > hide #34.1-3,5-13#!35-36#!34.4 atoms |
23463 | | |
23464 | | > show #34.1-3,5-13#!35-36#!34.4 cartoons |
23465 | | |
23466 | | > hide #!34 models |
23467 | | |
23468 | | > hide #!36 models |
23469 | | |
23470 | | > show #!16 models |
23471 | | |
23472 | | > show #!18 models |
23473 | | |
23474 | | > hide #!16 models |
23475 | | |
23476 | | > hide #!18 models |
23477 | | |
23478 | | > show #!20 models |
23479 | | |
23480 | | > hide #!20 models |
23481 | | |
23482 | | > show #!22 models |
23483 | | |
23484 | | > hide #!35 models |
23485 | | |
23486 | | > show #!36 models |
23487 | | |
23488 | | > show #!20 models |
23489 | | |
23490 | | > hide #!20 models |
23491 | | |
23492 | | > show #!18 models |
23493 | | |
23494 | | > hide #!22 models |
23495 | | |
23496 | | > hide #!18 models |
23497 | | |
23498 | | > hide #!36 models |
23499 | | |
23500 | | > split #35 chains |
23501 | | |
23502 | | Split COPI_soluble_job155_dhelix.pdb (#35) into 14 models |
23503 | | Chain information for COPI_soluble_job155_dhelix.pdb D #35.1 |
23504 | | --- |
23505 | | Chain | Description |
23506 | | D | No description available |
23507 | | |
23508 | | Chain information for COPI_soluble_job155_dhelix.pdb E #35.2 |
23509 | | --- |
23510 | | Chain | Description |
23511 | | E | No description available |
23512 | | |
23513 | | Chain information for COPI_soluble_job155_dhelix.pdb F #35.3 |
23514 | | --- |
23515 | | Chain | Description |
23516 | | F | No description available |
23517 | | |
23518 | | Chain information for COPI_soluble_job155_dhelix.pdb G #35.4 |
23519 | | --- |
23520 | | Chain | Description |
23521 | | G | No description available |
23522 | | |
23523 | | Chain information for COPI_soluble_job155_dhelix.pdb H #35.5 |
23524 | | --- |
23525 | | Chain | Description |
23526 | | H | No description available |
23527 | | |
23528 | | Chain information for COPI_soluble_job155_dhelix.pdb I #35.6 |
23529 | | --- |
23530 | | Chain | Description |
23531 | | I | No description available |
23532 | | |
23533 | | Chain information for COPI_soluble_job155_dhelix.pdb J #35.7 |
23534 | | --- |
23535 | | Chain | Description |
23536 | | J | No description available |
23537 | | |
23538 | | Chain information for COPI_soluble_job155_dhelix.pdb K #35.8 |
23539 | | --- |
23540 | | Chain | Description |
23541 | | K | No description available |
23542 | | |
23543 | | Chain information for COPI_soluble_job155_dhelix.pdb L #35.9 |
23544 | | --- |
23545 | | Chain | Description |
23546 | | L | No description available |
23547 | | |
23548 | | Chain information for COPI_soluble_job155_dhelix.pdb M #35.10 |
23549 | | --- |
23550 | | Chain | Description |
23551 | | M | No description available |
23552 | | |
23553 | | Chain information for COPI_soluble_job155_dhelix.pdb N #35.11 |
23554 | | --- |
23555 | | Chain | Description |
23556 | | N | No description available |
23557 | | |
23558 | | Chain information for COPI_soluble_job155_dhelix.pdb O #35.12 |
23559 | | --- |
23560 | | Chain | Description |
23561 | | O | No description available |
23562 | | |
23563 | | Chain information for COPI_soluble_job155_dhelix.pdb P #35.13 |
23564 | | --- |
23565 | | Chain | Description |
23566 | | P | No description available |
23567 | | |
23568 | | Chain information for COPI_soluble_job155_dhelix.pdb Q #35.14 |
23569 | | --- |
23570 | | Chain | Description |
23571 | | Q | No description available |
23572 | | |
23573 | | |
23574 | | > split #36 chains |
23575 | | |
23576 | | Split COPI_soluble_job160_dhelix.pdb (#36) into 14 models |
23577 | | Chain information for COPI_soluble_job160_dhelix.pdb D #36.1 |
23578 | | --- |
23579 | | Chain | Description |
23580 | | D | No description available |
23581 | | |
23582 | | Chain information for COPI_soluble_job160_dhelix.pdb E #36.2 |
23583 | | --- |
23584 | | Chain | Description |
23585 | | E | No description available |
23586 | | |
23587 | | Chain information for COPI_soluble_job160_dhelix.pdb F #36.3 |
23588 | | --- |
23589 | | Chain | Description |
23590 | | F | No description available |
23591 | | |
23592 | | Chain information for COPI_soluble_job160_dhelix.pdb G #36.4 |
23593 | | --- |
23594 | | Chain | Description |
23595 | | G | No description available |
23596 | | |
23597 | | Chain information for COPI_soluble_job160_dhelix.pdb H #36.5 |
23598 | | --- |
23599 | | Chain | Description |
23600 | | H | No description available |
23601 | | |
23602 | | Chain information for COPI_soluble_job160_dhelix.pdb I #36.6 |
23603 | | --- |
23604 | | Chain | Description |
23605 | | I | No description available |
23606 | | |
23607 | | Chain information for COPI_soluble_job160_dhelix.pdb J #36.7 |
23608 | | --- |
23609 | | Chain | Description |
23610 | | J | No description available |
23611 | | |
23612 | | Chain information for COPI_soluble_job160_dhelix.pdb K #36.8 |
23613 | | --- |
23614 | | Chain | Description |
23615 | | K | No description available |
23616 | | |
23617 | | Chain information for COPI_soluble_job160_dhelix.pdb L #36.9 |
23618 | | --- |
23619 | | Chain | Description |
23620 | | L | No description available |
23621 | | |
23622 | | Chain information for COPI_soluble_job160_dhelix.pdb M #36.10 |
23623 | | --- |
23624 | | Chain | Description |
23625 | | M | No description available |
23626 | | |
23627 | | Chain information for COPI_soluble_job160_dhelix.pdb N #36.11 |
23628 | | --- |
23629 | | Chain | Description |
23630 | | N | No description available |
23631 | | |
23632 | | Chain information for COPI_soluble_job160_dhelix.pdb O #36.12 |
23633 | | --- |
23634 | | Chain | Description |
23635 | | O | No description available |
23636 | | |
23637 | | Chain information for COPI_soluble_job160_dhelix.pdb P #36.13 |
23638 | | --- |
23639 | | Chain | Description |
23640 | | P | No description available |
23641 | | |
23642 | | Chain information for COPI_soluble_job160_dhelix.pdb Q #36.14 |
23643 | | --- |
23644 | | Chain | Description |
23645 | | Q | No description available |
23646 | | |
23647 | | |
23648 | | > open |
23649 | | > /Users/becca/Desktop/Postdoc/Phenix/W20_MM_cyto_COPI/ResolveCryoEM_32/J4028_denmod_map.ccp4 |
23650 | | |
23651 | | Opened J4028_denmod_map.ccp4 as #37, grid size 98,99,135, pixel 1.19, shown at |
23652 | | level 0.424, step 1, values float32 |
23653 | | |
23654 | | > volume #37 level 0.2276 |
23655 | | |
23656 | | > hide #!35 models |
23657 | | |
23658 | | > hide #!36 models |
23659 | | |
23660 | | > show #!35.14 models |
23661 | | |
23662 | | > show #!35.13 models |
23663 | | |
23664 | | > show #35.12 models |
23665 | | |
23666 | | > show #!35.11 models |
23667 | | |
23668 | | > show #35.10 models |
23669 | | |
23670 | | > show #35.9 models |
23671 | | |
23672 | | > show #35.8 models |
23673 | | |
23674 | | > show #!35.7 models |
23675 | | |
23676 | | > show #!35.6 models |
23677 | | |
23678 | | > show #35.5 models |
23679 | | |
23680 | | > show #35.4 models |
23681 | | |
23682 | | > show #35.3 models |
23683 | | |
23684 | | > show #35.2 models |
23685 | | |
23686 | | > show #35.1 models |
23687 | | |
23688 | | > hide #!35 models |
23689 | | |
23690 | | > volume #37 level 0.1206 |
23691 | | |
23692 | | > close #37 |
23693 | | |
23694 | | > show #!16 models |
23695 | | |
23696 | | > show #!31 models |
23697 | | |
23698 | | > show #!35 models |
23699 | | |
23700 | | > hide #35.1 models |
23701 | | |
23702 | | > hide #35.2 models |
23703 | | |
23704 | | > hide #35.3 models |
23705 | | |
23706 | | > hide #35.4 models |
23707 | | |
23708 | | > hide #35.5 models |
23709 | | |
23710 | | > hide #!35.6 models |
23711 | | |
23712 | | > hide #!35.7 models |
23713 | | |
23714 | | > hide #35.8 models |
23715 | | |
23716 | | > hide #35.9 models |
23717 | | |
23718 | | > show #35.9 models |
23719 | | |
23720 | | > hide #35.10 models |
23721 | | |
23722 | | > show #35.10 models |
23723 | | |
23724 | | > hide #35.10 models |
23725 | | |
23726 | | > show #35.10 models |
23727 | | |
23728 | | > hide #!35.11 models |
23729 | | |
23730 | | > show #!35.11 models |
23731 | | |
23732 | | > hide #35.12 models |
23733 | | |
23734 | | > hide #!35.13 models |
23735 | | |
23736 | | > hide #!35.14 models |
23737 | | |
23738 | | > fitmap #35.9 inMap #16 |
23739 | | |
23740 | | Fit molecule COPI_soluble_job155_dhelix.pdb L (#35.9) to map |
23741 | | W20_J4026_consensus_resampled.mrc (#16) using 1163 atoms |
23742 | | average map value = 0.3245, steps = 56 |
23743 | | shifted from previous position = 1.07 |
23744 | | rotated from previous position = 3.98 degrees |
23745 | | atoms outside contour = 440, contour level = 0.25963 |
23746 | | |
23747 | | Position of COPI_soluble_job155_dhelix.pdb L (#35.9) relative to |
23748 | | W20_J4026_consensus_resampled.mrc (#16) coordinates: |
23749 | | Matrix rotation and translation |
23750 | | -0.90987894 0.24236896 -0.33671591 435.50395252 |
23751 | | 0.06606031 -0.71661672 -0.69433170 604.82381864 |
23752 | | -0.40958070 -0.65400135 0.63602349 262.39041137 |
23753 | | Axis 0.20683441 0.37368744 -0.90419977 |
23754 | | Axis point 263.30917938 345.30538105 0.00000000 |
23755 | | Rotation angle (degrees) 174.40510072 |
23756 | | Shift along axis 78.83891898 |
23757 | | |
23758 | | |
23759 | | > fitmap #35.10 inMap #16 |
23760 | | |
23761 | | Fit molecule COPI_soluble_job155_dhelix.pdb M (#35.10) to map |
23762 | | W20_J4026_consensus_resampled.mrc (#16) using 2212 atoms |
23763 | | average map value = 0.3306, steps = 92 |
23764 | | shifted from previous position = 2.46 |
23765 | | rotated from previous position = 6.1 degrees |
23766 | | atoms outside contour = 710, contour level = 0.25963 |
23767 | | |
23768 | | Position of COPI_soluble_job155_dhelix.pdb M (#35.10) relative to |
23769 | | W20_J4026_consensus_resampled.mrc (#16) coordinates: |
23770 | | Matrix rotation and translation |
23771 | | -0.91287694 0.27628102 -0.30054033 410.29266882 |
23772 | | -0.00773154 -0.74776328 -0.66392040 629.67159952 |
23773 | | -0.40816163 -0.60375398 0.68475193 227.42100406 |
23774 | | Axis 0.19432248 0.34758987 -0.91728952 |
23775 | | Axis point 254.63604518 341.25031476 0.00000000 |
23776 | | Rotation angle (degrees) 171.09417945 |
23777 | | Shift along axis 89.98565405 |
23778 | | |
23779 | | |
23780 | | > fitmap #35.11 inMap #16 |
23781 | | |
23782 | | Fit molecule COPI_soluble_job155_dhelix.pdb N (#35.11) to map |
23783 | | W20_J4026_consensus_resampled.mrc (#16) using 1064 atoms |
23784 | | average map value = 0.3024, steps = 52 |
23785 | | shifted from previous position = 1.19 |
23786 | | rotated from previous position = 1.61 degrees |
23787 | | atoms outside contour = 298, contour level = 0.25963 |
23788 | | |
23789 | | Position of COPI_soluble_job155_dhelix.pdb N (#35.11) relative to |
23790 | | W20_J4026_consensus_resampled.mrc (#16) coordinates: |
23791 | | Matrix rotation and translation |
23792 | | -0.93792901 0.21017775 -0.27588855 435.11677592 |
23793 | | 0.06068204 -0.68374702 -0.72719166 606.90260953 |
23794 | | -0.34147748 -0.69879563 0.62855215 261.30949009 |
23795 | | Axis 0.17136788 0.39582419 -0.90219524 |
23796 | | Axis point 255.61353944 351.07885546 0.00000000 |
23797 | | Rotation angle (degrees) 175.24753470 |
23798 | | Shift along axis 79.03959464 |
23799 | | |
23800 | | |
23801 | | > hide #31.13 models |
23802 | | |
23803 | | > hide #31.12 models |
23804 | | |
23805 | | > show #31.12 models |
23806 | | |
23807 | | > select add #31.12 |
23808 | | |
23809 | | 1420 atoms, 1441 bonds, 177 residues, 1 model selected |
23810 | | |
23811 | | > view matrix models |
23812 | | > #31.12,-0.23372,-0.97024,-0.063385,254.71,-0.96642,0.23897,-0.094492,266.72,0.10683,0.039172,-0.99351,199.68 |
23813 | | |
23814 | | > fitmap #31.12 inMap #16 |
23815 | | |
23816 | | Fit molecule COPI_cyto_J4026_model L (#31.12) to map |
23817 | | W20_J4026_consensus_resampled.mrc (#16) using 1420 atoms |
23818 | | average map value = 0.2882, steps = 136 |
23819 | | shifted from previous position = 4.88 |
23820 | | rotated from previous position = 24.5 degrees |
23821 | | atoms outside contour = 593, contour level = 0.25963 |
23822 | | |
23823 | | Position of COPI_cyto_J4026_model L (#31.12) relative to |
23824 | | W20_J4026_consensus_resampled.mrc (#16) coordinates: |
23825 | | Matrix rotation and translation |
23826 | | -0.11780824 -0.96709435 0.22549890 202.70773070 |
23827 | | -0.96549277 0.16465999 0.20176923 232.00719445 |
23828 | | -0.23226052 -0.19394747 -0.95312090 262.37235731 |
23829 | | Axis -0.65397629 0.75651041 0.00264684 |
23830 | | Axis point 228.48470686 0.00000000 107.10492224 |
23831 | | Rotation angle (degrees) 162.38937332 |
23832 | | Shift along axis 43.64426578 |
23833 | | |
23834 | | |
23835 | | > select subtract #31.12 |
23836 | | |
23837 | | Nothing selected |
23838 | | |
23839 | | > show #31.13 models |
23840 | | |
23841 | | > hide #31.13 models |
23842 | | |
23843 | | > hide #31.12 models |
23844 | | |
23845 | | > hide #31.11 models |
23846 | | |
23847 | | > hide #31.10 models |
23848 | | |
23849 | | > hide #31.9 models |
23850 | | |
23851 | | > hide #31.8 models |
23852 | | |
23853 | | > hide #31.7 models |
23854 | | |
23855 | | > hide #31.6 models |
23856 | | |
23857 | | > hide #31.5 models |
23858 | | |
23859 | | > hide #!31.4 models |
23860 | | |
23861 | | > hide #31.3 models |
23862 | | |
23863 | | > hide #31.2 models |
23864 | | |
23865 | | > hide #31.1 models |
23866 | | |
23867 | | > hide #!16 models |
23868 | | |
23869 | | > show #31.13 models |
23870 | | |
23871 | | > hide #35.9 models |
23872 | | |
23873 | | > hide #!35.11 models |
23874 | | |
23875 | | > hide #35.10 models |
23876 | | |
23877 | | > ui mousemode right select |
23878 | | |
23879 | | Drag select of 228 residues |
23880 | | |
23881 | | > select up |
23882 | | |
23883 | | 2019 atoms, 2046 bonds, 257 residues, 1 model selected |
23884 | | |
23885 | | > delete sel |
23886 | | |
23887 | | > show #35.10 models |
23888 | | |
23889 | | Drag select of 6 residues, 1 pseudobonds |
23890 | | |
23891 | | > select up |
23892 | | |
23893 | | 61 atoms, 60 bonds, 1 pseudobond, 8 residues, 2 models selected |
23894 | | |
23895 | | > delete sel |
23896 | | |
23897 | | Drag select of 3 residues |
23898 | | |
23899 | | > select up |
23900 | | |
23901 | | 130 atoms, 130 bonds, 17 residues, 1 model selected |
23902 | | |
23903 | | > delete sel |
23904 | | |
23905 | | > fitmap #31.13 inMap #16 |
23906 | | |
23907 | | Fit molecule COPI_cyto_J4026_model M (#31.13) to map |
23908 | | W20_J4026_consensus_resampled.mrc (#16) using 2342 atoms |
23909 | | average map value = 0.3254, steps = 72 |
23910 | | shifted from previous position = 2.25 |
23911 | | rotated from previous position = 5.92 degrees |
23912 | | atoms outside contour = 789, contour level = 0.25963 |
23913 | | |
23914 | | Position of COPI_cyto_J4026_model M (#31.13) relative to |
23915 | | W20_J4026_consensus_resampled.mrc (#16) coordinates: |
23916 | | Matrix rotation and translation |
23917 | | -0.14741636 -0.97480443 0.16740596 215.74618933 |
23918 | | -0.97007287 0.17551559 0.16778831 236.68236999 |
23919 | | -0.19294315 -0.13766124 -0.97150519 252.55946622 |
23920 | | Axis -0.64657421 0.76278534 0.01001574 |
23921 | | Axis point 227.98326837 0.00000000 105.57175219 |
23922 | | Rotation angle (degrees) 166.33727095 |
23923 | | Shift along axis 43.57149027 |
23924 | | |
23925 | | |
23926 | | > hide #!31.13 models |
23927 | | |
23928 | | > show #!31.13 models |
23929 | | |
23930 | | > show #!16 models |
23931 | | |
23932 | | > open "/Users/becca/Desktop/Postdoc/COPI notebooks and resources/Alphafold |
23933 | | > predictions/fold_20250401_ct_alpha1_350_gamma1_350/fold_20250401_ct_alpha1_350_gamma1_350_model_0.cif" |
23934 | | |
23935 | | Chain information for fold_20250401_ct_alpha1_350_gamma1_350_model_0.cif #37 |
23936 | | --- |
23937 | | Chain | Description |
23938 | | A | . |
23939 | | B | . |
23940 | | |
23941 | | Computing secondary structure |
23942 | | |
23943 | | > ui tool show Matchmaker |
23944 | | |
23945 | | > matchmaker #37 to #31.1 |
23946 | | |
23947 | | Parameters |
23948 | | --- |
23949 | | Chain pairing | bb |
23950 | | Alignment algorithm | Needleman-Wunsch |
23951 | | Similarity matrix | BLOSUM-62 |
23952 | | SS fraction | 0.3 |
23953 | | Gap open (HH/SS/other) | 18/18/6 |
23954 | | Gap extend | 1 |
23955 | | SS matrix | | | H | S | O |
23956 | | ---|---|---|--- |
23957 | | H | 6 | -9 | -6 |
23958 | | S | | 6 | -6 |
23959 | | O | | | 4 |
23960 | | Iteration cutoff | 2 |
23961 | | |
23962 | | Matchmaker COPI_cyto_J4026_model A, chain A (#31.1) with |
23963 | | fold_20250401_ct_alpha1_350_gamma1_350_model_0.cif, chain A (#37), sequence |
23964 | | alignment score = 1357.6 |
23965 | | RMSD between 278 pruned atom pairs is 0.471 angstroms; (across all 313 pairs: |
23966 | | 6.653) |
23967 | | |
23968 | | |
23969 | | > open "/Users/becca/Desktop/Postdoc/COPI notebooks and resources/Alphafold |
23970 | | > predictions/fold_20250401_ct_alpha1_350_gamma1_50/fold_20250401_ct_alpha1_350_gamma1_50_model_0.cif" |
23971 | | |
23972 | | Chain information for fold_20250401_ct_alpha1_350_gamma1_50_model_0.cif #38 |
23973 | | --- |
23974 | | Chain | Description |
23975 | | A | . |
23976 | | B | . |
23977 | | |
23978 | | Computing secondary structure |
23979 | | |
23980 | | > ui tool show Matchmaker |
23981 | | |
23982 | | > matchmaker #38 to #24.1 |
23983 | | |
23984 | | Parameters |
23985 | | --- |
23986 | | Chain pairing | bb |
23987 | | Alignment algorithm | Needleman-Wunsch |
23988 | | Similarity matrix | BLOSUM-62 |
23989 | | SS fraction | 0.3 |
23990 | | Gap open (HH/SS/other) | 18/18/6 |
23991 | | Gap extend | 1 |
23992 | | SS matrix | | | H | S | O |
23993 | | ---|---|---|--- |
23994 | | H | 6 | -9 | -6 |
23995 | | S | | 6 | -6 |
23996 | | O | | | 4 |
23997 | | Iteration cutoff | 2 |
23998 | | |
23999 | | Matchmaker COPI_cyto_for_J4027_v1 A, chain A (#24.1) with |
24000 | | fold_20250401_ct_alpha1_350_gamma1_50_model_0.cif, chain A (#38), sequence |
24001 | | alignment score = 1354 |
24002 | | RMSD between 279 pruned atom pairs is 0.445 angstroms; (across all 313 pairs: |
24003 | | 6.676) |
24004 | | |
24005 | | |
24006 | | > hide #!16 models |
24007 | | |
24008 | | > open "/Users/becca/Desktop/Postdoc/COPI notebooks and resources/Alphafold |
24009 | | > predictions/fold_2025_01_17_alpha1_330gamma1_330zeta/fold_2025_01_17_alpha1_330gamma1_330zeta_model_0.cif" |
24010 | | |
24011 | | Chain information for fold_2025_01_17_alpha1_330gamma1_330zeta_model_0.cif #39 |
24012 | | --- |
24013 | | Chain | Description |
24014 | | A | . |
24015 | | B | . |
24016 | | C | . |
24017 | | |
24018 | | Computing secondary structure |
24019 | | |
24020 | | > ui tool show Matchmaker |
24021 | | |
24022 | | > matchmaker #39 to #24.1 |
24023 | | |
24024 | | Parameters |
24025 | | --- |
24026 | | Chain pairing | bb |
24027 | | Alignment algorithm | Needleman-Wunsch |
24028 | | Similarity matrix | BLOSUM-62 |
24029 | | SS fraction | 0.3 |
24030 | | Gap open (HH/SS/other) | 18/18/6 |
24031 | | Gap extend | 1 |
24032 | | SS matrix | | | H | S | O |
24033 | | ---|---|---|--- |
24034 | | H | 6 | -9 | -6 |
24035 | | S | | 6 | -6 |
24036 | | O | | | 4 |
24037 | | Iteration cutoff | 2 |
24038 | | |
24039 | | Matchmaker COPI_cyto_for_J4027_v1 A, chain A (#24.1) with |
24040 | | fold_2025_01_17_alpha1_330gamma1_330zeta_model_0.cif, chain B (#39), sequence |
24041 | | alignment score = 1688.7 |
24042 | | RMSD between 283 pruned atom pairs is 0.307 angstroms; (across all 318 pairs: |
24043 | | 4.917) |
24044 | | |
24045 | | |
24046 | | > hide #38 models |
24047 | | |
24048 | | > hide #37 models |
24049 | | |
24050 | | > fitmap #39 inMap #16 |
24051 | | |
24052 | | Fit molecule fold_2025_01_17_alpha1_330gamma1_330zeta_model_0.cif (#39) to map |
24053 | | W20_J4026_consensus_resampled.mrc (#16) using 6676 atoms |
24054 | | average map value = 0.2973, steps = 112 |
24055 | | shifted from previous position = 5.99 |
24056 | | rotated from previous position = 14.7 degrees |
24057 | | atoms outside contour = 2828, contour level = 0.25963 |
24058 | | |
24059 | | Position of fold_2025_01_17_alpha1_330gamma1_330zeta_model_0.cif (#39) |
24060 | | relative to W20_J4026_consensus_resampled.mrc (#16) coordinates: |
24061 | | Matrix rotation and translation |
24062 | | 0.75236864 0.61844958 -0.22685138 135.89900591 |
24063 | | 0.54481378 -0.77776674 -0.31345947 141.53555984 |
24064 | | -0.37029633 0.11224532 -0.92210716 89.13598609 |
24065 | | Axis 0.93516725 0.31511280 -0.16175949 |
24066 | | Axis point 0.00000000 40.80728347 61.99249889 |
24067 | | Rotation angle (degrees) 166.84366143 |
24068 | | Shift along axis 157.26937502 |
24069 | | |
24070 | | |
24071 | | > split #39 chains |
24072 | | |
24073 | | Split fold_2025_01_17_alpha1_330gamma1_330zeta_model_0.cif (#39) into 3 models |
24074 | | Chain information for fold_2025_01_17_alpha1_330gamma1_330zeta_model_0.cif A |
24075 | | #39.1 |
24076 | | --- |
24077 | | Chain | Description |
24078 | | A | No description available |
24079 | | |
24080 | | Chain information for fold_2025_01_17_alpha1_330gamma1_330zeta_model_0.cif B |
24081 | | #39.2 |
24082 | | --- |
24083 | | Chain | Description |
24084 | | B | No description available |
24085 | | |
24086 | | Chain information for fold_2025_01_17_alpha1_330gamma1_330zeta_model_0.cif C |
24087 | | #39.3 |
24088 | | --- |
24089 | | Chain | Description |
24090 | | C | No description available |
24091 | | |
24092 | | |
24093 | | > show #!16 models |
24094 | | |
24095 | | > hide #!31.13 models |
24096 | | |
24097 | | > hide #39.2 models |
24098 | | |
24099 | | > show #39.2 models |
24100 | | |
24101 | | > hide #39.2 models |
24102 | | |
24103 | | > hide #39.1 models |
24104 | | |
24105 | | > show #39.1 models |
24106 | | |
24107 | | > hide #39.3 models |
24108 | | |
24109 | | > show #31.12 models |
24110 | | |
24111 | | > show #31.1 models |
24112 | | |
24113 | | > fitmap #39.1 inMap #16 |
24114 | | |
24115 | | Fit molecule fold_2025_01_17_alpha1_330gamma1_330zeta_model_0.cif A (#39.1) to |
24116 | | map W20_J4026_consensus_resampled.mrc (#16) using 2567 atoms |
24117 | | average map value = 0.332, steps = 92 |
24118 | | shifted from previous position = 1.08 |
24119 | | rotated from previous position = 8.07 degrees |
24120 | | atoms outside contour = 837, contour level = 0.25963 |
24121 | | |
24122 | | Position of fold_2025_01_17_alpha1_330gamma1_330zeta_model_0.cif A (#39.1) |
24123 | | relative to W20_J4026_consensus_resampled.mrc (#16) coordinates: |
24124 | | Matrix rotation and translation |
24125 | | 0.83404122 0.50639742 -0.21894494 135.37114526 |
24126 | | 0.43420964 -0.84733835 -0.30574452 138.37406207 |
24127 | | -0.34034868 0.15993553 -0.92659775 89.77783066 |
24128 | | Axis 0.95695074 0.24947901 -0.14834252 |
24129 | | Axis point 0.00000000 44.30433155 61.88415197 |
24130 | | Rotation angle (degrees) 165.91774988 |
24131 | | Shift along axis 150.74707237 |
24132 | | |
24133 | | |
24134 | | > hide #!16 models |
24135 | | |
24136 | | > hide #31.12 models |
24137 | | |
24138 | | > show #35.9 models |
24139 | | |
24140 | | > hide #35.9 models |
24141 | | |
24142 | | > show #35.9 models |
24143 | | |
24144 | | > show #!16 models |
24145 | | |
24146 | | > hide #!16 models |
24147 | | |
24148 | | > hide #35.9 models |
24149 | | |
24150 | | > hide #35.10 models |
24151 | | |
24152 | | > show #35.9 models |
24153 | | |
24154 | | > hide #39.1 models |
24155 | | |
24156 | | Drag select of 11 residues |
24157 | | |
24158 | | > select up |
24159 | | |
24160 | | 130 atoms, 130 bonds, 17 residues, 1 model selected |
24161 | | |
24162 | | > delete sel |
24163 | | |
24164 | | > show #39.1 models |
24165 | | |
24166 | | > select #35.9/L:317 |
24167 | | |
24168 | | 9 atoms, 8 bonds, 1 residue, 1 model selected |
24169 | | |
24170 | | > delete sel |
24171 | | |
24172 | | > hide #39.1 models |
24173 | | |
24174 | | Drag select of 4 residues |
24175 | | |
24176 | | > delete sel |
24177 | | |
24178 | | Drag select of 5 residues |
24179 | | |
24180 | | > delete sel |
24181 | | |
24182 | | > select #35.9/L:327 |
24183 | | |
24184 | | 8 atoms, 7 bonds, 1 residue, 1 model selected |
24185 | | |
24186 | | > delete sel |
24187 | | |
24188 | | > show #39.1 models |
24189 | | |
24190 | | > hide #39.1 models |
24191 | | |
24192 | | > select #35.9/L:328 |
24193 | | |
24194 | | 8 atoms, 7 bonds, 1 residue, 1 model selected |
24195 | | |
24196 | | > delete sel |
24197 | | |
24198 | | > select #35.9/L:329 |
24199 | | |
24200 | | 8 atoms, 7 bonds, 1 residue, 1 model selected |
24201 | | |
24202 | | > delete sel |
24203 | | |
24204 | | > show #39.1 models |
24205 | | |
24206 | | > select #35.9/L:330 |
24207 | | |
24208 | | 9 atoms, 8 bonds, 1 residue, 1 model selected |
24209 | | |
24210 | | > delete sel |
24211 | | |
24212 | | > show #!16 models |
24213 | | |
24214 | | > fitmap #35.9 inMap #16 |
24215 | | |
24216 | | Fit molecule COPI_soluble_job155_dhelix.pdb L (#35.9) to map |
24217 | | W20_J4026_consensus_resampled.mrc (#16) using 930 atoms |
24218 | | average map value = 0.3516, steps = 52 |
24219 | | shifted from previous position = 0.499 |
24220 | | rotated from previous position = 6.25 degrees |
24221 | | atoms outside contour = 295, contour level = 0.25963 |
24222 | | |
24223 | | Position of COPI_soluble_job155_dhelix.pdb L (#35.9) relative to |
24224 | | W20_J4026_consensus_resampled.mrc (#16) coordinates: |
24225 | | Matrix rotation and translation |
24226 | | -0.93642912 0.16612187 -0.30903727 462.34398677 |
24227 | | 0.11986381 -0.67634146 -0.72677018 585.05727704 |
24228 | | -0.32974714 -0.71761114 0.61343384 270.00020679 |
24229 | | Axis 0.17783383 0.40210728 -0.89815637 |
24230 | | Axis point 261.88005557 349.74957427 0.00000000 |
24231 | | Rotation angle (degrees) 178.52437469 |
24232 | | Shift along axis 74.97378580 |
24233 | | |
24234 | | |
24235 | | > show #35.8 models |
24236 | | |
24237 | | > hide #35.8 models |
24238 | | |
24239 | | > show #!35.11 models |
24240 | | |
24241 | | > show #31.12 models |
24242 | | |
24243 | | > show #31.11 models |
24244 | | |
24245 | | > show #31.10 models |
24246 | | |
24247 | | > show #31.9 models |
24248 | | |
24249 | | > show #31.8 models |
24250 | | |
24251 | | > show #31.7 models |
24252 | | |
24253 | | > show #31.6 models |
24254 | | |
24255 | | > show #31.5 models |
24256 | | |
24257 | | > show #!31.4 models |
24258 | | |
24259 | | > show #31.3 models |
24260 | | |
24261 | | > show #31.2 models |
24262 | | |
24263 | | > combine #31.1 #31.2 #31.3 #31.4 #31.5 #31.6 #31.7 #31.8 #31.9 #31.10 #31.11 |
24264 | | > #31.12 #39.1 #35.9 #35.11 |
24265 | | |
24266 | | Remapping chain ID 'A' in fold_2025_01_17_alpha1_330gamma1_330zeta_model_0.cif |
24267 | | A #39.1 to 'M' |
24268 | | Remapping chain ID 'L' in COPI_soluble_job155_dhelix.pdb L #35.9 to 'N' |
24269 | | Remapping chain ID 'N' in COPI_soluble_job155_dhelix.pdb N #35.11 to 'O' |
24270 | | |
24271 | | > rename #40 COPI_cyto_J4026_model |
24272 | | |
24273 | | > hide #!40 models |
24274 | | |
24275 | | > show #!40 models |
24276 | | |
24277 | | > hide #!40 models |
24278 | | |
24279 | | > hide #!16 models |
24280 | | |
24281 | | > show #!18 models |
24282 | | |
24283 | | > fitmap #35.11 inMap #18 |
24284 | | |
24285 | | Fit molecule COPI_soluble_job155_dhelix.pdb N (#35.11) to map |
24286 | | W20_J4027_consensus_resampled.mrc (#18) using 1064 atoms |
24287 | | average map value = 0.345, steps = 52 |
24288 | | shifted from previous position = 0.96 |
24289 | | rotated from previous position = 4.13 degrees |
24290 | | atoms outside contour = 305, contour level = 0.25259 |
24291 | | |
24292 | | Position of COPI_soluble_job155_dhelix.pdb N (#35.11) relative to |
24293 | | W20_J4027_consensus_resampled.mrc (#18) coordinates: |
24294 | | Matrix rotation and translation |
24295 | | -0.95797163 0.17569317 -0.22676476 434.71371371 |
24296 | | 0.05774245 -0.65621750 -0.75235923 607.00290226 |
24297 | | -0.28099138 -0.73383275 0.61849280 258.09556438 |
24298 | | Axis 0.14127895 0.41352064 -0.89946703 |
24299 | | Axis point 248.09458414 355.10604389 0.00000000 |
24300 | | Rotation angle (degrees) 176.24059016 |
24301 | | Shift along axis 80.27567222 |
24302 | | |
24303 | | |
24304 | | > fitmap #35.9 inMap #18 |
24305 | | |
24306 | | Fit molecule COPI_soluble_job155_dhelix.pdb L (#35.9) to map |
24307 | | W20_J4027_consensus_resampled.mrc (#18) using 930 atoms |
24308 | | average map value = 0.3717, steps = 64 |
24309 | | shifted from previous position = 4.09 |
24310 | | rotated from previous position = 12 degrees |
24311 | | atoms outside contour = 279, contour level = 0.25259 |
24312 | | |
24313 | | Position of COPI_soluble_job155_dhelix.pdb L (#35.9) relative to |
24314 | | W20_J4027_consensus_resampled.mrc (#18) coordinates: |
24315 | | Matrix rotation and translation |
24316 | | -0.98236672 0.03849512 -0.18295835 476.12367349 |
24317 | | 0.12352589 -0.60093803 -0.78969288 580.42963487 |
24318 | | -0.14034595 -0.79836809 0.58558637 250.87293860 |
24319 | | Axis -0.09083469 -0.44617724 0.89032293 |
24320 | | Axis point 243.54419066 359.36795920 0.00000000 |
24321 | | Rotation angle (degrees) 177.26292686 |
24322 | | Shift along axis -78.86510835 |
24323 | | |
24324 | | |
24325 | | > fitmap #39.1 inMap #18 |
24326 | | |
24327 | | Fit molecule fold_2025_01_17_alpha1_330gamma1_330zeta_model_0.cif A (#39.1) to |
24328 | | map W20_J4027_consensus_resampled.mrc (#18) using 2567 atoms |
24329 | | average map value = 0.2497, steps = 140 |
24330 | | shifted from previous position = 20.2 |
24331 | | rotated from previous position = 23.5 degrees |
24332 | | atoms outside contour = 1358, contour level = 0.25259 |
24333 | | |
24334 | | Position of fold_2025_01_17_alpha1_330gamma1_330zeta_model_0.cif A (#39.1) |
24335 | | relative to W20_J4027_consensus_resampled.mrc (#18) coordinates: |
24336 | | Matrix rotation and translation |
24337 | | 0.71011541 0.46894974 -0.52518782 133.17594101 |
24338 | | 0.50811821 -0.85767517 -0.07879844 140.50399717 |
24339 | | -0.48739306 -0.21090151 -0.84733026 102.37727978 |
24340 | | Axis -0.92459305 -0.26452658 0.27414118 |
24341 | | Axis point 0.00000000 53.88234017 68.89098797 |
24342 | | Rotation angle (degrees) 175.90338628 |
24343 | | Shift along axis -132.23476323 |
24344 | | |
24345 | | |
24346 | | > select add #39.1 |
24347 | | |
24348 | | 2567 atoms, 2609 bonds, 330 residues, 1 model selected |
24349 | | |
24350 | | > ui mousemode right "translate selected models" |
24351 | | |
24352 | | > view matrix models |
24353 | | > #39.1,0.71012,0.46895,-0.52519,127.98,0.50812,-0.85768,-0.078798,150.01,-0.48739,-0.2109,-0.84733,99.315 |
24354 | | |
24355 | | > fitmap #39.1 inMap #18 |
24356 | | |
24357 | | Fit molecule fold_2025_01_17_alpha1_330gamma1_330zeta_model_0.cif A (#39.1) to |
24358 | | map W20_J4027_consensus_resampled.mrc (#18) using 2567 atoms |
24359 | | average map value = 0.2908, steps = 104 |
24360 | | shifted from previous position = 3.89 |
24361 | | rotated from previous position = 15.2 degrees |
24362 | | atoms outside contour = 938, contour level = 0.25259 |
24363 | | |
24364 | | Position of fold_2025_01_17_alpha1_330gamma1_330zeta_model_0.cif A (#39.1) |
24365 | | relative to W20_J4027_consensus_resampled.mrc (#18) coordinates: |
24366 | | Matrix rotation and translation |
24367 | | 0.65350179 0.33145691 -0.68049373 127.41590893 |
24368 | | 0.52879246 -0.84317616 0.09712103 144.98236935 |
24369 | | -0.54158466 -0.42330872 -0.72628905 97.39971457 |
24370 | | Axis -0.90721165 -0.24214590 0.34399476 |
24371 | | Axis point 0.00000000 66.60547701 63.44217524 |
24372 | | Rotation angle (degrees) 163.33177472 |
24373 | | Shift along axis -117.19509172 |
24374 | | |
24375 | | |
24376 | | > hide #31.12 models |
24377 | | |
24378 | | > hide #!31 models |
24379 | | |
24380 | | > show #!32 models |
24381 | | |
24382 | | > hide #32.13 models |
24383 | | |
24384 | | > fitmap #32.12 inMap #18 |
24385 | | |
24386 | | Fit molecule COPI_cyto_J4027_model L (#32.12) to map |
24387 | | W20_J4027_consensus_resampled.mrc (#18) using 1420 atoms |
24388 | | average map value = 0.249, steps = 84 |
24389 | | shifted from previous position = 0.209 |
24390 | | rotated from previous position = 0.257 degrees |
24391 | | atoms outside contour = 664, contour level = 0.25259 |
24392 | | |
24393 | | Position of COPI_cyto_J4027_model L (#32.12) relative to |
24394 | | W20_J4027_consensus_resampled.mrc (#18) coordinates: |
24395 | | Matrix rotation and translation |
24396 | | -0.36763985 -0.91576410 -0.16191685 275.33631018 |
24397 | | -0.79632880 0.22007538 0.56340686 164.04444754 |
24398 | | -0.48031386 0.33606986 -0.81015780 242.91724000 |
24399 | | Axis -0.55576549 0.77837779 0.29198072 |
24400 | | Axis point 211.47624167 0.00000000 70.72859152 |
24401 | | Rotation angle (degrees) 168.19824647 |
24402 | | Shift along axis 45.59328469 |
24403 | | |
24404 | | |
24405 | | > view matrix models |
24406 | | > #39.1,0.6535,0.33146,-0.68049,127.38,0.52879,-0.84318,0.097121,144.98,-0.54158,-0.42331,-0.72629,97.387 |
24407 | | |
24408 | | > select subtract #39.1 |
24409 | | |
24410 | | Nothing selected |
24411 | | |
24412 | | > fitmap #32.5 inMap #18 |
24413 | | |
24414 | | Fit molecule COPI_cyto_J4027_model E (#32.5) to map |
24415 | | W20_J4027_consensus_resampled.mrc (#18) using 1876 atoms |
24416 | | average map value = 0.3307, steps = 28 |
24417 | | shifted from previous position = 0.0226 |
24418 | | rotated from previous position = 0.025 degrees |
24419 | | atoms outside contour = 588, contour level = 0.25259 |
24420 | | |
24421 | | Position of COPI_cyto_J4027_model E (#32.5) relative to |
24422 | | W20_J4027_consensus_resampled.mrc (#18) coordinates: |
24423 | | Matrix rotation and translation |
24424 | | -0.38917297 -0.90711538 -0.16026883 277.45394634 |
24425 | | -0.76472866 0.22115752 0.60521025 157.23067335 |
24426 | | -0.51355087 0.35809364 -0.77976565 242.80657971 |
24427 | | Axis -0.54426642 0.77809236 0.31360219 |
24428 | | Axis point 210.56184964 0.00000000 67.30315702 |
24429 | | Rotation angle (degrees) 166.87842423 |
24430 | | Shift along axis 47.47579529 |
24431 | | |
24432 | | |
24433 | | > combine #32.1 #32.2 #32.3 #32.4 #32.5 #32.6 #32.7 #32.8 #32.9 #32.10 #32.11 |
24434 | | > #32.12 #39.1 #35.9 #35.11 |
24435 | | |
24436 | | Remapping chain ID 'A' in fold_2025_01_17_alpha1_330gamma1_330zeta_model_0.cif |
24437 | | A #39.1 to 'M' |
24438 | | Remapping chain ID 'L' in COPI_soluble_job155_dhelix.pdb L #35.9 to 'N' |
24439 | | Remapping chain ID 'N' in COPI_soluble_job155_dhelix.pdb N #35.11 to 'O' |
24440 | | |
24441 | | > rename #41 COPI_cyto_J4017_model |
24442 | | |
24443 | | > hide #39.1 models |
24444 | | |
24445 | | > show #39.1 models |
24446 | | |
24447 | | > hide #!41 models |
24448 | | |
24449 | | > hide #!32 models |
24450 | | |
24451 | | > hide #!18 models |
24452 | | |
24453 | | > show #!20 models |
24454 | | |
24455 | | > fitmap #35.9 inMap #10 |
24456 | | |
24457 | | Fit molecule COPI_soluble_job155_dhelix.pdb L (#35.9) to map |
24458 | | W20_J4023_consensus_resampled.mrc (#10) using 930 atoms |
24459 | | average map value = 0.2393, steps = 52 |
24460 | | shifted from previous position = 2.69 |
24461 | | rotated from previous position = 6.1 degrees |
24462 | | atoms outside contour = 585, contour level = 0.259 |
24463 | | |
24464 | | Position of COPI_soluble_job155_dhelix.pdb L (#35.9) relative to |
24465 | | W20_J4023_consensus_resampled.mrc (#10) coordinates: |
24466 | | Matrix rotation and translation |
24467 | | -0.98125869 0.10123862 -0.16395770 446.50000833 |
24468 | | 0.05661840 -0.66183176 -0.74751126 606.96394850 |
24469 | | -0.18418942 -0.74278494 0.64369619 224.44341669 |
24470 | | Axis 0.09602403 0.41104534 -0.90654350 |
24471 | | Axis point 239.00882222 351.39277051 0.00000000 |
24472 | | Rotation angle (degrees) 178.58980379 |
24473 | | Shift along axis 88.89671334 |
24474 | | |
24475 | | |
24476 | | > fitmap #35.11 inMap #10 |
24477 | | |
24478 | | Fit molecule COPI_soluble_job155_dhelix.pdb N (#35.11) to map |
24479 | | W20_J4023_consensus_resampled.mrc (#10) using 1064 atoms |
24480 | | average map value = 0.2705, steps = 48 |
24481 | | shifted from previous position = 1.17 |
24482 | | rotated from previous position = 3.69 degrees |
24483 | | atoms outside contour = 361, contour level = 0.259 |
24484 | | |
24485 | | Position of COPI_soluble_job155_dhelix.pdb N (#35.11) relative to |
24486 | | W20_J4023_consensus_resampled.mrc (#10) coordinates: |
24487 | | Matrix rotation and translation |
24488 | | -0.95447536 0.16472623 -0.24868064 445.72556094 |
24489 | | 0.09748552 -0.61564640 -0.78196936 592.79673482 |
24490 | | -0.28191020 -0.77061325 0.57156090 289.62220826 |
24491 | | Axis 0.14970170 0.43804800 -0.88639909 |
24492 | | Axis point 253.33667423 363.30405703 0.00000000 |
24493 | | Rotation angle (degrees) 177.82629912 |
24494 | | Shift along axis 69.67843860 |
24495 | | |
24496 | | |
24497 | | > fitmap #39.1 inMap #10 |
24498 | | |
24499 | | Fit molecule fold_2025_01_17_alpha1_330gamma1_330zeta_model_0.cif A (#39.1) to |
24500 | | map W20_J4023_consensus_resampled.mrc (#10) using 2567 atoms |
24501 | | average map value = 0.197, steps = 180 |
24502 | | shifted from previous position = 18.8 |
24503 | | rotated from previous position = 39.4 degrees |
24504 | | atoms outside contour = 2326, contour level = 0.259 |
24505 | | |
24506 | | Position of fold_2025_01_17_alpha1_330gamma1_330zeta_model_0.cif A (#39.1) |
24507 | | relative to W20_J4023_consensus_resampled.mrc (#10) coordinates: |
24508 | | Matrix rotation and translation |
24509 | | 0.77937618 0.53787625 -0.32134390 138.00151844 |
24510 | | 0.38455520 -0.81555599 -0.43241846 137.27793171 |
24511 | | -0.49466156 0.21344218 -0.84246803 102.09006947 |
24512 | | Axis 0.94140147 0.25262649 -0.22347958 |
24513 | | Axis point 0.00000000 37.55487421 76.95697173 |
24514 | | Rotation angle (degrees) 159.93832539 |
24515 | | Shift along axis 141.77982886 |
24516 | | |
24517 | | |
24518 | | > select add #39.1 |
24519 | | |
24520 | | 2567 atoms, 2609 bonds, 330 residues, 1 model selected |
24521 | | |
24522 | | > view matrix models |
24523 | | > #39.1,0.77938,0.53788,-0.32134,133.03,0.38456,-0.81556,-0.43242,136.21,-0.49466,0.21344,-0.84247,87.787 |
24524 | | |
24525 | | > fitmap #39.1 inMap #10 |
24526 | | |
24527 | | Fit molecule fold_2025_01_17_alpha1_330gamma1_330zeta_model_0.cif A (#39.1) to |
24528 | | map W20_J4023_consensus_resampled.mrc (#10) using 2567 atoms |
24529 | | average map value = 0.197, steps = 148 |
24530 | | shifted from previous position = 15.2 |
24531 | | rotated from previous position = 0.0116 degrees |
24532 | | atoms outside contour = 2326, contour level = 0.259 |
24533 | | |
24534 | | Position of fold_2025_01_17_alpha1_330gamma1_330zeta_model_0.cif A (#39.1) |
24535 | | relative to W20_J4023_consensus_resampled.mrc (#10) coordinates: |
24536 | | Matrix rotation and translation |
24537 | | 0.77933517 0.53786894 -0.32145559 138.00257192 |
24538 | | 0.38460571 -0.81561140 -0.43226900 137.27863469 |
24539 | | -0.49468691 0.21324878 -0.84250212 102.09071968 |
24540 | | Axis 0.94139228 0.25263228 -0.22351177 |
24541 | | Axis point 0.00000000 37.56089500 76.95480112 |
24542 | | Rotation angle (degrees) 159.94922815 |
24543 | | Shift along axis 141.77709241 |
24544 | | |
24545 | | |
24546 | | > view matrix models |
24547 | | > #39.1,0.77934,0.53787,-0.32146,130.24,0.38461,-0.81561,-0.43227,141.73,-0.49469,0.21325,-0.8425,88.079 |
24548 | | |
24549 | | > view matrix models |
24550 | | > #39.1,0.77934,0.53787,-0.32146,135.31,0.38461,-0.81561,-0.43227,140.11,-0.49469,0.21325,-0.8425,84.463 |
24551 | | |
24552 | | > fitmap #39.1 inMap #10 |
24553 | | |
24554 | | Fit molecule fold_2025_01_17_alpha1_330gamma1_330zeta_model_0.cif A (#39.1) to |
24555 | | map W20_J4023_consensus_resampled.mrc (#10) using 2567 atoms |
24556 | | average map value = 0.197, steps = 172 |
24557 | | shifted from previous position = 18.1 |
24558 | | rotated from previous position = 0.0154 degrees |
24559 | | atoms outside contour = 2326, contour level = 0.259 |
24560 | | |
24561 | | Position of fold_2025_01_17_alpha1_330gamma1_330zeta_model_0.cif A (#39.1) |
24562 | | relative to W20_J4023_consensus_resampled.mrc (#10) coordinates: |
24563 | | Matrix rotation and translation |
24564 | | 0.77924517 0.53791820 -0.32159133 138.00026966 |
24565 | | 0.38472109 -0.81564702 -0.43209909 137.28065889 |
24566 | | -0.49473897 0.21298817 -0.84253747 102.08755822 |
24567 | | Axis 0.94137014 0.25267283 -0.22355918 |
24568 | | Axis point 0.00000000 37.56685145 76.95023483 |
24569 | | Rotation angle (degrees) 159.96268257 |
24570 | | Shift along axis 141.77381369 |
24571 | | |
24572 | | |
24573 | | > view matrix models |
24574 | | > #39.1,0.77925,0.53792,-0.32159,128.09,0.38472,-0.81565,-0.4321,135.26,-0.49474,0.21299,-0.84254,82.235 |
24575 | | |
24576 | | > view matrix models |
24577 | | > #39.1,0.77925,0.53792,-0.32159,135.47,0.38472,-0.81565,-0.4321,136.17,-0.49474,0.21299,-0.84254,76.831 |
24578 | | |
24579 | | > view matrix models |
24580 | | > #39.1,0.77925,0.53792,-0.32159,139.06,0.38472,-0.81565,-0.4321,133.92,-0.49474,0.21299,-0.84254,83.878 |
24581 | | |
24582 | | > view matrix models |
24583 | | > #39.1,0.77925,0.53792,-0.32159,133.59,0.38472,-0.81565,-0.4321,136.36,-0.49474,0.21299,-0.84254,86.161 |
24584 | | |
24585 | | > view matrix models |
24586 | | > #39.1,0.77925,0.53792,-0.32159,137.64,0.38472,-0.81565,-0.4321,140.68,-0.49474,0.21299,-0.84254,88.092 |
24587 | | |
24588 | | > ui mousemode right "rotate selected models" |
24589 | | |
24590 | | > view matrix models |
24591 | | > #39.1,0.88557,0.46445,-0.0069167,134.98,0.45496,-0.87029,-0.18867,138.57,-0.093649,0.16394,-0.98202,91.009 |
24592 | | |
24593 | | > fitmap #39.1 inMap #10 |
24594 | | |
24595 | | Fit molecule fold_2025_01_17_alpha1_330gamma1_330zeta_model_0.cif A (#39.1) to |
24596 | | map W20_J4023_consensus_resampled.mrc (#10) using 2567 atoms |
24597 | | average map value = 0.197, steps = 144 |
24598 | | shifted from previous position = 19.4 |
24599 | | rotated from previous position = 24.7 degrees |
24600 | | atoms outside contour = 2327, contour level = 0.259 |
24601 | | |
24602 | | Position of fold_2025_01_17_alpha1_330gamma1_330zeta_model_0.cif A (#39.1) |
24603 | | relative to W20_J4023_consensus_resampled.mrc (#10) coordinates: |
24604 | | Matrix rotation and translation |
24605 | | 0.77925174 0.53807988 -0.32130481 137.99460111 |
24606 | | 0.38488292 -0.81549555 -0.43224086 137.29386764 |
24607 | | -0.49460275 0.21315971 -0.84257407 102.10795439 |
24608 | | Axis 0.94137004 0.25276936 -0.22345043 |
24609 | | Axis point 0.00000000 37.55980635 76.95624722 |
24610 | | Rotation angle (degrees) 159.95253140 |
24611 | | Shift along axis 141.79160050 |
24612 | | |
24613 | | |
24614 | | > view matrix models |
24615 | | > #39.1,0.68606,0.65344,-0.3199,137.9,0.57062,-0.75605,-0.32059,137.24,-0.45135,0.037399,-0.89156,102.26 |
24616 | | |
24617 | | > ui mousemode right "translate selected models" |
24618 | | |
24619 | | > view matrix models |
24620 | | > #39.1,0.68606,0.65344,-0.3199,131.56,0.57062,-0.75605,-0.32059,145.97,-0.45135,0.037399,-0.89156,83.123 |
24621 | | |
24622 | | > view matrix models |
24623 | | > #39.1,0.68606,0.65344,-0.3199,134.43,0.57062,-0.75605,-0.32059,139.6,-0.45135,0.037399,-0.89156,83.125 |
24624 | | |
24625 | | > view matrix models |
24626 | | > #39.1,0.68606,0.65344,-0.3199,130.69,0.57062,-0.75605,-0.32059,139.63,-0.45135,0.037399,-0.89156,81.892 |
24627 | | |
24628 | | > view matrix models |
24629 | | > #39.1,0.68606,0.65344,-0.3199,132.35,0.57062,-0.75605,-0.32059,136.91,-0.45135,0.037399,-0.89156,85.265 |
24630 | | |
24631 | | > view matrix models |
24632 | | > #39.1,0.68606,0.65344,-0.3199,127.51,0.57062,-0.75605,-0.32059,139.3,-0.45135,0.037399,-0.89156,87.993 |
24633 | | |
24634 | | > view matrix models |
24635 | | > #39.1,0.68606,0.65344,-0.3199,131.61,0.57062,-0.75605,-0.32059,143.53,-0.45135,0.037399,-0.89156,89.59 |
24636 | | |
24637 | | > fitmap #39.1 inMap #10 |
24638 | | |
24639 | | Fit molecule fold_2025_01_17_alpha1_330gamma1_330zeta_model_0.cif A (#39.1) to |
24640 | | map W20_J4023_consensus_resampled.mrc (#10) using 2567 atoms |
24641 | | average map value = 0.197, steps = 144 |
24642 | | shifted from previous position = 14.5 |
24643 | | rotated from previous position = 13.3 degrees |
24644 | | atoms outside contour = 2327, contour level = 0.259 |
24645 | | |
24646 | | Position of fold_2025_01_17_alpha1_330gamma1_330zeta_model_0.cif A (#39.1) |
24647 | | relative to W20_J4023_consensus_resampled.mrc (#10) coordinates: |
24648 | | Matrix rotation and translation |
24649 | | 0.77919349 0.53804611 -0.32150254 137.99342007 |
24650 | | 0.38489409 -0.81557949 -0.43207249 137.29343954 |
24651 | | -0.49468580 0.21292364 -0.84258500 102.10246297 |
24652 | | Axis 0.94135648 0.25275684 -0.22352173 |
24653 | | Axis point 0.00000000 37.56820104 76.95313719 |
24654 | | Rotation angle (degrees) 159.96533135 |
24655 | | Shift along axis 141.78073688 |
24656 | | |
24657 | | |
24658 | | > select subtract #39.1 |
24659 | | |
24660 | | Nothing selected |
24661 | | |
24662 | | > hide #39.1 models |
24663 | | |
24664 | | > show #!33 models |
24665 | | |
24666 | | > hide #33.1 models |
24667 | | |
24668 | | > hide #33.2 models |
24669 | | |
24670 | | > hide #33.3 models |
24671 | | |
24672 | | > hide #!33.4 models |
24673 | | |
24674 | | > hide #33.5 models |
24675 | | |
24676 | | > hide #33.6 models |
24677 | | |
24678 | | > hide #33.7 models |
24679 | | |
24680 | | > hide #33.8 models |
24681 | | |
24682 | | > hide #33.9 models |
24683 | | |
24684 | | > hide #33.10 models |
24685 | | |
24686 | | > hide #33.11 models |
24687 | | |
24688 | | > hide #33.12 models |
24689 | | |
24690 | | > fitmap #33.13 inMap #10 |
24691 | | |
24692 | | Fit molecule COPI_cyto_J4028_model M (#33.13) to map |
24693 | | W20_J4023_consensus_resampled.mrc (#10) using 4552 atoms |
24694 | | average map value = 0.2144, steps = 128 |
24695 | | shifted from previous position = 5.65 |
24696 | | rotated from previous position = 7.59 degrees |
24697 | | atoms outside contour = 3456, contour level = 0.259 |
24698 | | |
24699 | | Position of COPI_cyto_J4028_model M (#33.13) relative to |
24700 | | W20_J4023_consensus_resampled.mrc (#10) coordinates: |
24701 | | Matrix rotation and translation |
24702 | | -0.35039846 -0.93657228 -0.00730075 262.48644706 |
24703 | | -0.86127456 0.31914510 0.39541441 178.27483489 |
24704 | | -0.36800418 0.14484055 -0.91847382 254.98975169 |
24705 | | Axis -0.56232261 0.80946867 0.16897857 |
24706 | | Axis point 210.46623440 0.00000000 89.03589265 |
24707 | | Rotation angle (degrees) 167.12630425 |
24708 | | Shift along axis 39.79363149 |
24709 | | |
24710 | | |
24711 | | > hide #!20 models |
24712 | | |
24713 | | > hide #!35.11 models |
24714 | | |
24715 | | > show #!35.11 models |
24716 | | |
24717 | | > hide #!35.11 models |
24718 | | |
24719 | | > hide #35.9 models |
24720 | | |
24721 | | > show #35.9 models |
24722 | | |
24723 | | > hide #33.13 models |
24724 | | |
24725 | | > hide #35.9 models |
24726 | | |
24727 | | > show #33.13 models |
24728 | | |
24729 | | > ui mousemode right select |
24730 | | |
24731 | | Drag select of 242 residues |
24732 | | |
24733 | | > select up |
24734 | | |
24735 | | 2060 atoms, 2089 bonds, 262 residues, 1 model selected |
24736 | | |
24737 | | > select clear |
24738 | | |
24739 | | Drag select of 128 residues |
24740 | | |
24741 | | > select up |
24742 | | |
24743 | | 1192 atoms, 1211 bonds, 151 residues, 1 model selected |
24744 | | |
24745 | | > delete sel |
24746 | | |
24747 | | > select clear |
24748 | | |
24749 | | Drag select of 5 residues |
24750 | | Drag select of 16 residues |
24751 | | Drag select of 64 residues, 1 pseudobonds |
24752 | | |
24753 | | > select up |
24754 | | |
24755 | | 587 atoms, 592 bonds, 1 pseudobond, 73 residues, 2 models selected |
24756 | | |
24757 | | > delete sel |
24758 | | |
24759 | | Drag select of 4 residues, 1 pseudobonds |
24760 | | |
24761 | | > delete sel |
24762 | | |
24763 | | Drag select of 1 pseudobonds |
24764 | | |
24765 | | > delete sel |
24766 | | |
24767 | | > select add #33.13 |
24768 | | |
24769 | | 2744 atoms, 2786 bonds, 1 pseudobond, 354 residues, 2 models selected |
24770 | | |
24771 | | > select subtract #33.13 |
24772 | | |
24773 | | Nothing selected |
24774 | | |
24775 | | > combine #30.13 |
24776 | | |
24777 | | > hide #!33.13 models |
24778 | | |
24779 | | Drag select of 172 residues |
24780 | | |
24781 | | > select up |
24782 | | |
24783 | | 1391 atoms, 1413 bonds, 176 residues, 1 model selected |
24784 | | |
24785 | | > delete sel |
24786 | | |
24787 | | Drag select of 24 residues |
24788 | | |
24789 | | > select up |
24790 | | |
24791 | | 388 atoms, 392 bonds, 48 residues, 1 model selected |
24792 | | |
24793 | | > delete sel |
24794 | | |
24795 | | Drag select of 15 residues, 1 pseudobonds |
24796 | | |
24797 | | > select up |
24798 | | |
24799 | | 269 atoms, 268 bonds, 1 pseudobond, 37 residues, 2 models selected |
24800 | | |
24801 | | > select clear |
24802 | | |
24803 | | Drag select of 9 residues, 1 pseudobonds |
24804 | | |
24805 | | > select up |
24806 | | |
24807 | | 155 atoms, 154 bonds, 1 pseudobond, 21 residues, 2 models selected |
24808 | | |
24809 | | > delete sel |
24810 | | |
24811 | | Drag select of 7 residues |
24812 | | |
24813 | | > select up |
24814 | | |
24815 | | 136 atoms, 136 bonds, 19 residues, 1 model selected |
24816 | | |
24817 | | > select clear |
24818 | | |
24819 | | > fitmap #42 inMap #20 |
24820 | | |
24821 | | Fit molecule copy of COPI_cyto_J4025_model M (#42) to map |
24822 | | W20_J4028_consensus_resampled.mrc (#20) using 2618 atoms |
24823 | | average map value = 0.306, steps = 180 |
24824 | | shifted from previous position = 23.6 |
24825 | | rotated from previous position = 33.1 degrees |
24826 | | atoms outside contour = 931, contour level = 0.25474 |
24827 | | |
24828 | | Position of copy of COPI_cyto_J4025_model M (#42) relative to |
24829 | | W20_J4028_consensus_resampled.mrc (#20) coordinates: |
24830 | | Matrix rotation and translation |
24831 | | -0.14963361 -0.97522083 0.16295437 216.33302484 |
24832 | | -0.96633931 0.17912766 0.18466631 233.37303521 |
24833 | | -0.20928006 -0.12983692 -0.96919773 254.43371461 |
24834 | | Axis -0.64527959 0.76372913 0.01822260 |
24835 | | Axis point 227.29901935 0.00000000 104.64971477 |
24836 | | Rotation angle (degrees) 165.89525591 |
24837 | | Shift along axis 43.27494515 |
24838 | | |
24839 | | |
24840 | | > show #!20 models |
24841 | | |
24842 | | > show #35.9 models |
24843 | | |
24844 | | > hide #35.9 models |
24845 | | |
24846 | | > show #35.9 models |
24847 | | |
24848 | | > fitmap #35.9 inMap #20 |
24849 | | |
24850 | | Fit molecule COPI_soluble_job155_dhelix.pdb L (#35.9) to map |
24851 | | W20_J4028_consensus_resampled.mrc (#20) using 930 atoms |
24852 | | average map value = 0.3762, steps = 56 |
24853 | | shifted from previous position = 2.58 |
24854 | | rotated from previous position = 5.97 degrees |
24855 | | atoms outside contour = 300, contour level = 0.25474 |
24856 | | |
24857 | | Position of COPI_soluble_job155_dhelix.pdb L (#35.9) relative to |
24858 | | W20_J4028_consensus_resampled.mrc (#20) coordinates: |
24859 | | Matrix rotation and translation |
24860 | | -0.95707237 0.18100601 -0.22638311 433.92940718 |
24861 | | 0.05310912 -0.65830260 -0.75087756 607.96749602 |
24862 | | -0.28494194 -0.73066717 0.62043016 258.74353581 |
24863 | | Axis 0.14221667 0.41206734 -0.89998607 |
24864 | | Axis point 248.77691524 354.86529873 0.00000000 |
24865 | | Rotation angle (degrees) 175.92541914 |
24866 | | Shift along axis 79.36996533 |
24867 | | |
24868 | | |
24869 | | > show #35.8 models |
24870 | | |
24871 | | > hide #35.8 models |
24872 | | |
24873 | | > show #!35.11 models |
24874 | | |
24875 | | > fitmap #35.11 inMap #20 |
24876 | | |
24877 | | Fit molecule COPI_soluble_job155_dhelix.pdb N (#35.11) to map |
24878 | | W20_J4028_consensus_resampled.mrc (#20) using 1064 atoms |
24879 | | average map value = 0.3378, steps = 88 |
24880 | | shifted from previous position = 0.716 |
24881 | | rotated from previous position = 7.18 degrees |
24882 | | atoms outside contour = 284, contour level = 0.25474 |
24883 | | |
24884 | | Position of COPI_soluble_job155_dhelix.pdb N (#35.11) relative to |
24885 | | W20_J4028_consensus_resampled.mrc (#20) coordinates: |
24886 | | Matrix rotation and translation |
24887 | | -0.94111577 0.21871586 -0.25780707 426.29452223 |
24888 | | 0.03665885 -0.69203983 -0.72092788 614.47706636 |
24889 | | -0.33609112 -0.68792750 0.64327172 250.03704434 |
24890 | | Axis 0.16425992 0.38966017 -0.90619183 |
24891 | | Axis point 251.98330593 349.53072602 0.00000000 |
24892 | | Rotation angle (degrees) 174.23481815 |
24893 | | Shift along axis 82.87881842 |
24894 | | |
24895 | | |
24896 | | > hide #!20 models |
24897 | | |
24898 | | > show #!20 models |
24899 | | |
24900 | | > hide #!20 models |
24901 | | |
24902 | | > select #35.9/L:331 |
24903 | | |
24904 | | 8 atoms, 7 bonds, 1 residue, 1 model selected |
24905 | | |
24906 | | > show #!20 models |
24907 | | |
24908 | | > hide #!20 models |
24909 | | |
24910 | | > delete sel |
24911 | | |
24912 | | > select #35.9/L:332 |
24913 | | |
24914 | | 8 atoms, 7 bonds, 1 residue, 1 model selected |
24915 | | |
24916 | | > delete sel |
24917 | | |
24918 | | > select #35.9/L:333 |
24919 | | |
24920 | | 7 atoms, 6 bonds, 1 residue, 1 model selected |
24921 | | |
24922 | | > delete sel |
24923 | | |
24924 | | > select #35.9/L:334 |
24925 | | |
24926 | | 7 atoms, 6 bonds, 1 residue, 1 model selected |
24927 | | |
24928 | | > delete sel |
24929 | | |
24930 | | > select #35.9/L:335 |
24931 | | |
24932 | | 8 atoms, 7 bonds, 1 residue, 1 model selected |
24933 | | |
24934 | | > delete sel |
24935 | | |
24936 | | > select #35.9/L:336 |
24937 | | |
24938 | | 6 atoms, 5 bonds, 1 residue, 1 model selected |
24939 | | |
24940 | | > delete sel |
24941 | | |
24942 | | > select #35.9/L:337 |
24943 | | |
24944 | | 8 atoms, 7 bonds, 1 residue, 1 model selected |
24945 | | |
24946 | | > delete sel |
24947 | | |
24948 | | > fitmap #35.9 inMap #20 |
24949 | | |
24950 | | Fit molecule COPI_soluble_job155_dhelix.pdb L (#35.9) to map |
24951 | | W20_J4028_consensus_resampled.mrc (#20) using 878 atoms |
24952 | | average map value = 0.3829, steps = 44 |
24953 | | shifted from previous position = 0.138 |
24954 | | rotated from previous position = 1.65 degrees |
24955 | | atoms outside contour = 274, contour level = 0.25474 |
24956 | | |
24957 | | Position of COPI_soluble_job155_dhelix.pdb L (#35.9) relative to |
24958 | | W20_J4028_consensus_resampled.mrc (#20) coordinates: |
24959 | | Matrix rotation and translation |
24960 | | -0.96329901 0.17783180 -0.20107426 427.85994614 |
24961 | | 0.03340558 -0.66383109 -0.74713610 614.70583444 |
24962 | | -0.26634390 -0.72643247 0.63352726 246.72265827 |
24963 | | Axis 0.12953006 0.40835260 -0.90358736 |
24964 | | Axis point 244.47252749 353.86664751 0.00000000 |
24965 | | Rotation angle (degrees) 175.41613396 |
24966 | | Shift along axis 83.50197716 |
24967 | | |
24968 | | |
24969 | | > show #!20 models |
24970 | | |
24971 | | > show #33.1 models |
24972 | | |
24973 | | > show #33.2 models |
24974 | | |
24975 | | > show #33.3 models |
24976 | | |
24977 | | > show #!33.4 models |
24978 | | |
24979 | | > show #33.5 models |
24980 | | |
24981 | | > show #33.6 models |
24982 | | |
24983 | | > show #33.7 models |
24984 | | |
24985 | | > show #33.8 models |
24986 | | |
24987 | | > show #33.9 models |
24988 | | |
24989 | | > show #33.10 models |
24990 | | |
24991 | | > show #33.11 models |
24992 | | |
24993 | | > show #33.12 models |
24994 | | |
24995 | | > combine #33.1 #33.2 #33.3 #33.4 #33.5 #33.6 #33.7 #33.8 #33.9 #33.10 #33.11 |
24996 | | > #33.12 #41 #35.9 #35.11 |
24997 | | |
24998 | | Remapping chain ID 'A' in COPI_cyto_J4017_model #41 to 'P' |
24999 | | Remapping chain ID 'B' in COPI_cyto_J4017_model #41 to 'Q' |
25000 | | Remapping chain ID 'C' in COPI_cyto_J4017_model #41 to 'R' |
25001 | | Remapping chain ID 'D' in COPI_cyto_J4017_model #41 to 'S' |
25002 | | Remapping chain ID 'E' in COPI_cyto_J4017_model #41 to 'T' |
25003 | | Remapping chain ID 'F' in COPI_cyto_J4017_model #41 to 'U' |
25004 | | Remapping chain ID 'G' in COPI_cyto_J4017_model #41 to 'V' |
25005 | | Remapping chain ID 'H' in COPI_cyto_J4017_model #41 to 'W' |
25006 | | Remapping chain ID 'I' in COPI_cyto_J4017_model #41 to 'X' |
25007 | | Remapping chain ID 'J' in COPI_cyto_J4017_model #41 to 'Y' |
25008 | | Remapping chain ID 'K' in COPI_cyto_J4017_model #41 to 'Z' |
25009 | | Remapping chain ID 'L' in COPI_cyto_J4017_model #41 to 'a' |
25010 | | Remapping chain ID 'L' in COPI_soluble_job155_dhelix.pdb L #35.9 to 'b' |
25011 | | Remapping chain ID 'N' in COPI_soluble_job155_dhelix.pdb N #35.11 to 'c' |
25012 | | |
25013 | | > rename #41 COPI_cyto_J4027_model |
25014 | | |
25015 | | > rename #43 COPI_cyto_J4028_model |
25016 | | |
25017 | | > hide #!20 models |
25018 | | |
25019 | | > show #!22 models |
25020 | | |
25021 | | > hide #!43 models |
25022 | | |
25023 | | > show #!43 models |
25024 | | |
25025 | | > hide #!22 models |
25026 | | |
25027 | | > show #!20 models |
25028 | | |
25029 | | > hide #!20 models |
25030 | | |
25031 | | > hide #!43 models |
25032 | | |
25033 | | > hide #!42 models |
25034 | | |
25035 | | > hide #!33 models |
25036 | | |
25037 | | > show #!16 models |
25038 | | |
25039 | | > hide #35.9 models |
25040 | | |
25041 | | > show #35.9 models |
25042 | | |
25043 | | > hide #!35 models |
25044 | | |
25045 | | > show #!40 models |
25046 | | |
25047 | | > hide #!40 models |
25048 | | |
25049 | | > hide #!16 models |
25050 | | |
25051 | | > show #!18 models |
25052 | | |
25053 | | > show #!41 models |
25054 | | |
25055 | | > hide #!41 models |
25056 | | |
25057 | | > show #!43 models |
25058 | | |
25059 | | > hide #!18 models |
25060 | | |
25061 | | > show #!20 models |
25062 | | |
25063 | | > close #43 |
25064 | | |
25065 | | > show #!42 models |
25066 | | |
25067 | | > show #!35 models |
25068 | | |
25069 | | > show #!33 models |
25070 | | |
25071 | | > combine #33.1 #33.2 #33.3 #33.4 #33.5 #33.6 #33.7 #33.8 #33.9 #33.10 #33.11 |
25072 | | > #33.12 #42 #35.9 #35.11 |
25073 | | |
25074 | | Remapping chain ID 'L' in COPI_soluble_job155_dhelix.pdb L #35.9 to 'N' |
25075 | | Remapping chain ID 'N' in COPI_soluble_job155_dhelix.pdb N #35.11 to 'O' |
25076 | | |
25077 | | > rename #43 COPI_cyto_J4028_model |
25078 | | |
25079 | | > hide #!43 models |
25080 | | |
25081 | | > show #!43 models |
25082 | | |
25083 | | > hide #!42 models |
25084 | | |
25085 | | > hide #!35 models |
25086 | | |
25087 | | > hide #!33 models |
25088 | | |
25089 | | > hide #!43 models |
25090 | | |
25091 | | > show #!43 models |
25092 | | |
25093 | | > hide #!43 models |
25094 | | |
25095 | | > hide #!20 models |
25096 | | |
25097 | | > show #!22 models |
25098 | | |
25099 | | > show #!34 models |
25100 | | |
25101 | | > hide #34.13 models |
25102 | | |
25103 | | > show #!35 models |
25104 | | |
25105 | | > show #39.1 models |
25106 | | |
25107 | | > fitmap #35.9 inMap #22 |
25108 | | |
25109 | | Fit molecule COPI_soluble_job155_dhelix.pdb L (#35.9) to map |
25110 | | W20_J3988_consensus_resampled.mrc (#22) using 878 atoms |
25111 | | average map value = 0.3662, steps = 64 |
25112 | | shifted from previous position = 1.16 |
25113 | | rotated from previous position = 1.8 degrees |
25114 | | atoms outside contour = 302, contour level = 0.28583 |
25115 | | |
25116 | | Position of COPI_soluble_job155_dhelix.pdb L (#35.9) relative to |
25117 | | W20_J3988_consensus_resampled.mrc (#22) coordinates: |
25118 | | Matrix rotation and translation |
25119 | | -0.95887497 0.16676765 -0.22966788 440.72789628 |
25120 | | 0.05962226 -0.67277255 -0.73744307 605.37107525 |
25121 | | -0.27749589 -0.72080903 0.63516169 247.24016866 |
25122 | | Axis 0.14036124 0.40358181 -0.90411307 |
25123 | | Axis point 248.93620927 350.83028914 0.00000000 |
25124 | | Rotation angle (degrees) 176.60298227 |
25125 | | Shift along axis 82.64480207 |
25126 | | |
25127 | | |
25128 | | > fitmap #35.11 inMap #22 |
25129 | | |
25130 | | Fit molecule COPI_soluble_job155_dhelix.pdb N (#35.11) to map |
25131 | | W20_J3988_consensus_resampled.mrc (#22) using 1064 atoms |
25132 | | average map value = 0.384, steps = 72 |
25133 | | shifted from previous position = 0.949 |
25134 | | rotated from previous position = 1.29 degrees |
25135 | | atoms outside contour = 322, contour level = 0.28583 |
25136 | | |
25137 | | Position of COPI_soluble_job155_dhelix.pdb N (#35.11) relative to |
25138 | | W20_J3988_consensus_resampled.mrc (#22) coordinates: |
25139 | | Matrix rotation and translation |
25140 | | -0.93994555 0.23565898 -0.24691539 415.54469230 |
25141 | | 0.01481361 -0.69455698 -0.71928517 620.65417336 |
25142 | | -0.34100282 -0.67974661 0.64935478 246.72523257 |
25143 | | Axis 0.16251841 0.38673478 -0.90775767 |
25144 | | Axis point 249.64126104 349.36740731 0.00000000 |
25145 | | Rotation angle (degrees) 173.01304550 |
25146 | | Shift along axis 83.59549488 |
25147 | | |
25148 | | |
25149 | | > fitmap #39.1 inMap #22 |
25150 | | |
25151 | | Fit molecule fold_2025_01_17_alpha1_330gamma1_330zeta_model_0.cif A (#39.1) to |
25152 | | map W20_J3988_consensus_resampled.mrc (#22) using 2567 atoms |
25153 | | average map value = 0.2763, steps = 92 |
25154 | | shifted from previous position = 10.5 |
25155 | | rotated from previous position = 9.1 degrees |
25156 | | atoms outside contour = 1395, contour level = 0.28583 |
25157 | | |
25158 | | Position of fold_2025_01_17_alpha1_330gamma1_330zeta_model_0.cif A (#39.1) |
25159 | | relative to W20_J3988_consensus_resampled.mrc (#22) coordinates: |
25160 | | Matrix rotation and translation |
25161 | | 0.78195191 0.59824553 -0.17508140 136.85372786 |
25162 | | 0.46784009 -0.74887041 -0.46938125 138.09735041 |
25163 | | -0.41191852 0.28512347 -0.86546389 93.90913619 |
25164 | | Axis 0.94138597 0.29549867 -0.16270522 |
25165 | | Axis point 0.00000000 34.34868013 69.05412162 |
25166 | | Rotation angle (degrees) 156.37546309 |
25167 | | Shift along axis 154.36025614 |
25168 | | |
25169 | | |
25170 | | > fitmap #42 inMap #22 |
25171 | | |
25172 | | Fit molecule copy of COPI_cyto_J4025_model M (#42) to map |
25173 | | W20_J3988_consensus_resampled.mrc (#22) using 2618 atoms |
25174 | | average map value = 0.3079, steps = 52 |
25175 | | shifted from previous position = 1.27 |
25176 | | rotated from previous position = 2.32 degrees |
25177 | | atoms outside contour = 1148, contour level = 0.28583 |
25178 | | |
25179 | | Position of copy of COPI_cyto_J4025_model M (#42) relative to |
25180 | | W20_J3988_consensus_resampled.mrc (#22) coordinates: |
25181 | | Matrix rotation and translation |
25182 | | -0.16260849 -0.97678601 0.13945458 220.44563836 |
25183 | | -0.95843463 0.18994779 0.21289181 227.66250748 |
25184 | | -0.23443883 -0.09904008 -0.96707265 255.16502900 |
25185 | | Axis -0.64015894 0.76731881 0.03766144 |
25186 | | Axis point 225.62353549 0.00000000 102.00127381 |
25187 | | Rotation angle (degrees) 165.89874346 |
25188 | | Shift along axis 43.17936097 |
25189 | | |
25190 | | |
25191 | | > hide #39.1 models |
25192 | | |
25193 | | > show #!42 models |
25194 | | |
25195 | | > combine #34.1 #34.2 #34.3 #34.4 #34.5 #34.6 #34.7 #34.8 #34.9 #34.10 #34.11 |
25196 | | > #34.12 #42 #35.9 #35.11 |
25197 | | |
25198 | | Remapping chain ID 'L' in COPI_soluble_job155_dhelix.pdb L #35.9 to 'N' |
25199 | | Remapping chain ID 'N' in COPI_soluble_job155_dhelix.pdb N #35.11 to 'O' |
25200 | | |
25201 | | > rename #44 COPI_cyto_J3988_model |
25202 | | |
25203 | | > hide #!42 models |
25204 | | |
25205 | | > hide #!22 models |
25206 | | |
25207 | | > show #!24 models |
25208 | | |
25209 | | > hide #!44 models |
25210 | | |
25211 | | > hide #!35 models |
25212 | | |
25213 | | > hide #!24 models |
25214 | | |
25215 | | > hide #!34 models |
25216 | | |
25217 | | > show #!24 models |
25218 | | |
25219 | | > show #!1 models |
25220 | | |
25221 | | > hide #!1 models |
25222 | | |
25223 | | > show #!3 models |
25224 | | |
25225 | | > hide #!3 models |
25226 | | |
25227 | | > show #!4 models |
25228 | | |
25229 | | > hide #!24 models |
25230 | | |
25231 | | > show #!25 models |
25232 | | |
25233 | | > hide #!4 models |
25234 | | |
25235 | | > show #!5 models |
25236 | | |
25237 | | > hide #!5 models |
25238 | | |
25239 | | > show #!6 models |
25240 | | |
25241 | | > hide #!25 models |
25242 | | |
25243 | | > show #!26 models |
25244 | | |
25245 | | > hide #!26 models |
25246 | | |
25247 | | > show #!27 models |
25248 | | |
25249 | | > hide #!6 models |
25250 | | |
25251 | | > show #!8 models |
25252 | | |
25253 | | > hide #!27 models |
25254 | | |
25255 | | > close #35 |
25256 | | |
25257 | | > close #36 |
25258 | | |
25259 | | > close #37-39 |
25260 | | |
25261 | | > close #42 |
25262 | | |
25263 | | > combine #24.1 #24.2 #24.3 #24.4 #24.5 #24.6 #24.7 #24.8 #24.9 #24.10 #24.11 |
25264 | | > #24.12 #24.13 |
25265 | | |
25266 | | > hide #!8 models |
25267 | | |
25268 | | > show #!1 models |
25269 | | |
25270 | | > rename #35 COPI_cyto_J3623_model |
25271 | | |
25272 | | > hide #!35 models |
25273 | | |
25274 | | > show #!35 models |
25275 | | |
25276 | | > combine #25.1 #25.2 #25.3 #25.4 #25.5 #25.6 #25.7 #25.8 #25.9 #25.10 #25.11 |
25277 | | > #25.12 #25.13 |
25278 | | |
25279 | | > rename #36 COPI_cyto_J3987_model |
25280 | | |
25281 | | > combine #26.1 #26.2 #26.3 #26.4 #26.5 #26.6 #26.7 #26.8 #26.9 #26.10 #26.11 |
25282 | | > #26.12 #26.13 |
25283 | | |
25284 | | > rename #37 COPI_cyto_J4021_model |
25285 | | |
25286 | | > combine #27.1 #27.2 #27.3 #27.4 #27.5 #27.6 #27.7 #27.8 #27.9 #27.10 #27.11 |
25287 | | > #27.12 #27.13 |
25288 | | |
25289 | | > rename #38 COPI_cyto_J4022_model |
25290 | | |
25291 | | > combine #28.1 #28.2 #28.3 #28.4 #28.5 #28.6 #28.7 #28.8 #28.9 #28.10 #28.11 |
25292 | | > #28.12 #28.13 |
25293 | | |
25294 | | > rename #39 COPI_cyto_J4023_model |
25295 | | |
25296 | | > combine #29.1 #29.2 #29.3 #29.4 #29.5 #29.6 #29.7 #29.8 #29.9 #29.10 #29.11 |
25297 | | > #29.12 #29.13 |
25298 | | |
25299 | | > rename #42 COPI_cyto_J4024_model |
25300 | | |
25301 | | > combine #30.1 #30.2 #30.3 #30.4 #30.5 #30.6 #30.7 #30.8 #30.9 #30.10 #30.11 |
25302 | | > #30.12 #30.13 |
25303 | | |
25304 | | > hide #!42 models |
25305 | | |
25306 | | > hide #!39 models |
25307 | | |
25308 | | > hide #!38 models |
25309 | | |
25310 | | > hide #!37 models |
25311 | | |
25312 | | > hide #!36 models |
25313 | | |
25314 | | > hide #!35 models |
25315 | | |
25316 | | > rename #45 COPI_cyto_J4025_model |
25317 | | |
25318 | | > save COPI_cyto_J3623_model.pdb #35 |
25319 | | |
25320 | | > save COPI_cyto_J3987_model.pdb #36 |
25321 | | |
25322 | | > save COPI_cyto_J4021_model.pdb #37 |
25323 | | |
25324 | | > save COPI_cyto_J4022_model.pdb #38 |
25325 | | |
25326 | | > save COPI_cyto_J4023_model.pdb #39 |
25327 | | |
25328 | | > save COPI_cyto_J4026_model.pdb #40 |
25329 | | |
25330 | | > save COPI_cyto_J4027_model.pdb #41 |
25331 | | |
25332 | | > save COPI_cyto_J4024_model.pdb #42 |
25333 | | |
25334 | | > save COPI_cyto_J4028_model.pdb #43 |
25335 | | |
25336 | | > save COPI_cyto_J3988_model.pdb #44 |
25337 | | |
25338 | | > save COPI_cyto_J4025_model.pdb #45 |
25339 | | |
25340 | | > hide #!45 models |
25341 | | |
25342 | | > show #!5 models |
25343 | | |
25344 | | > hide #!1 models |
25345 | | |
25346 | | > hide #!5 models |
25347 | | |
25348 | | > show #!4 models |
25349 | | |
25350 | | > hide #!4 models |
25351 | | |
25352 | | > show #!6 models |
25353 | | |
25354 | | > hide #!6 models |
25355 | | |
25356 | | > show #!8 models |
25357 | | |
25358 | | > hide #!8 models |
25359 | | |
25360 | | > show #!10 models |
25361 | | |
25362 | | > hide #!10 models |
25363 | | |
25364 | | > show #!10 models |
25365 | | |
25366 | | > hide #!10 models |
25367 | | |
25368 | | > show #!6 models |
25369 | | |
25370 | | > show #!31 models |
25371 | | |
25372 | | > open |
25373 | | > /Users/becca/Desktop/Postdoc/Phenix/W20_MM_cyto_COPI/CombineFocusedMaps_33/composite_map.ccp4 |
25374 | | |
25375 | | Opened composite_map.ccp4 as #46, grid size 240,240,240, pixel 1.19, shown at |
25376 | | level 5.84, step 1, values float32 |
25377 | | |
25378 | | > rename #46 composite_map_J4021_J4026.ccp4 |
25379 | | |
25380 | | > hide #!46 models |
25381 | | |
25382 | | > show #!46 models |
25383 | | |
25384 | | > hide #!6 models |
25385 | | |
25386 | | > volume #46 level 4.356 |
25387 | | |
25388 | | > color #46 darkgrey models |
25389 | | |
25390 | | > color #46 #a9a9a9c3 models |
25391 | | |
25392 | | > color #46 #a9a9a9c0 models |
25393 | | |
25394 | | > color #46 #a9a9a9bf models |
25395 | | |
25396 | | > hide #!31 models |
25397 | | |
25398 | | > show #!40 models |
25399 | | |
25400 | | > save "/Users/becca/Desktop/Postdoc/COPI notebooks and |
25401 | | > resources/Processing/W20_MM_cyto_COPI/20250707_W20_COPI_cyto_alignments.cxs" |
25402 | | |
25403 | | > hide #!46 models |
25404 | | |
25405 | | > show #!46 models |
25406 | | |
25407 | | > hide #!46 models |
25408 | | |
25409 | | > open |
25410 | | > /Users/becca/Desktop/Postdoc/Phenix/W20_MM_cyto_COPI/J3987_ResolveCryoEM_34/J3987_denmod_map.ccp4 |
25411 | | |
25412 | | Opened J3987_denmod_map.ccp4 as #47, grid size 100,124,124, pixel 1.19, shown |
25413 | | at level 0.316, step 1, values float32 |
25414 | | |
25415 | | > volume #47 level 0.168 |
25416 | | |
25417 | | > volume #47 level 0.08306 |
25418 | | |
25419 | | > close #47 |
25420 | | |
25421 | | > open |
25422 | | > /Users/becca/Desktop/Postdoc/Phenix/W20_MM_cyto_COPI/J4021_ResolveCryoEM_35/J4021_denmod_map.ccp4 |
25423 | | |
25424 | | Opened J4021_denmod_map.ccp4 as #47, grid size 92,128,121, pixel 1.19, shown |
25425 | | at level 0.263, step 1, values float32 |
25426 | | |
25427 | | > volume #47 level 0.1546 |
25428 | | |
25429 | | > volume #47 level 0.0986 |
25430 | | |
25431 | | > close #47 |
25432 | | |
25433 | | > open |
25434 | | > /Users/becca/Desktop/Postdoc/Phenix/W20_MM_cyto_COPI/J4022_ResolveCryoEM_36/J4022_denmod_map.ccp4 |
25435 | | |
25436 | | Opened J4022_denmod_map.ccp4 as #47, grid size 92,126,116, pixel 1.19, shown |
25437 | | at level 0.256, step 1, values float32 |
25438 | | |
25439 | | > volume #47 level 0.1136 |
25440 | | |
25441 | | > close #47 |
25442 | | |
25443 | | > open |
25444 | | > /Users/becca/Desktop/Postdoc/Phenix/W20_MM_cyto_COPI/J4023_ResolveCryoEM_37/J4023_denmod_map.ccp4 |
25445 | | |
25446 | | Opened J4023_denmod_map.ccp4 as #47, grid size 92,125,117, pixel 1.19, shown |
25447 | | at level 0.26, step 1, values float32 |
25448 | | |
25449 | | > volume #47 level 0.06747 |
25450 | | |
25451 | | > open |
25452 | | > /Users/becca/Desktop/Postdoc/Phenix/W20_MM_cyto_COPI/J4024_ResolveCryoEM_38/J4024_denmod_map.ccp4 |
25453 | | |
25454 | | Opened J4024_denmod_map.ccp4 as #48, grid size 92,126,119, pixel 1.19, shown |
25455 | | at level 0.262, step 1, values float32 |
25456 | | |
25457 | | > close #47 |
25458 | | |
25459 | | > volume #48 level 0.1111 |
25460 | | |
25461 | | > close #48 |
25462 | | |
25463 | | > open |
25464 | | > /Users/becca/Desktop/Postdoc/Phenix/W20_MM_cyto_COPI/J4025_ResolveCryoEM_40/J4025_denmod_map.ccp4 |
25465 | | |
25466 | | Opened J4025_denmod_map.ccp4 as #47, grid size 91,128,120, pixel 1.19, shown |
25467 | | at level 0.235, step 1, values float32 |
25468 | | |
25469 | | > volume #47 level 0.1354 |
25470 | | |
25471 | | > volume #47 level 0.09666 |
25472 | | |
25473 | | > close #47 |
25474 | | |
25475 | | > save "/Users/becca/Desktop/Postdoc/COPI notebooks and |
25476 | | > resources/Processing/W20_MM_cyto_COPI/20250707_W20_COPI_cyto_alignments.cxs" |
25477 | | |
25478 | | ——— End of log from Mon Jul 7 09:47:13 2025 ——— |
25479 | | |
25480 | | > view name session-start |
25481 | | |
25482 | | opened ChimeraX session |
25483 | | |
25484 | | > show #!46 models |
25485 | | |
25486 | | > open |
25487 | | > /Users/becca/Desktop/Postdoc/Phenix/W20_MM_cyto_COPI/J4021_J4027_CombineFocusedMaps_41/J4021_J4027_composite_map.ccp4 |
25488 | | |
25489 | | Opened J4021_J4027_composite_map.ccp4 as #47, grid size 240,240,240, pixel |
25490 | | 1.19, shown at level 5.81, step 1, values float32 |
25491 | | |
25492 | | > color #47 darkgrey models |
25493 | | |
25494 | | > color #47 #a9a9a9c1 models |
25495 | | |
25496 | | > hide #!47 models |
25497 | | |
25498 | | > hide #!46 models |
25499 | | |
25500 | | > hide #!40 models |
25501 | | |
25502 | | > show #!40 models |
25503 | | |
25504 | | > hide #!40 models |
25505 | | |
25506 | | > combine #40 |
25507 | | |
25508 | | > split #48 chains |
25509 | | |
25510 | | Split copy of COPI_cyto_J4026_model (#48) into 15 models |
25511 | | Chain information for copy of COPI_cyto_J4026_model A #48.1 |
25512 | | --- |
25513 | | Chain | Description |
25514 | | A | No description available |
25515 | | |
25516 | | Chain information for copy of COPI_cyto_J4026_model B #48.2 |
25517 | | --- |
25518 | | Chain | Description |
25519 | | B | No description available |
25520 | | |
25521 | | Chain information for copy of COPI_cyto_J4026_model C #48.3 |
25522 | | --- |
25523 | | Chain | Description |
25524 | | C | No description available |
25525 | | |
25526 | | Chain information for copy of COPI_cyto_J4026_model D #48.4 |
25527 | | --- |
25528 | | Chain | Description |
25529 | | D | No description available |
25530 | | |
25531 | | Chain information for copy of COPI_cyto_J4026_model E #48.5 |
25532 | | --- |
25533 | | Chain | Description |
25534 | | E | No description available |
25535 | | |
25536 | | Chain information for copy of COPI_cyto_J4026_model F #48.6 |
25537 | | --- |
25538 | | Chain | Description |
25539 | | F | No description available |
25540 | | |
25541 | | Chain information for copy of COPI_cyto_J4026_model G #48.7 |
25542 | | --- |
25543 | | Chain | Description |
25544 | | G | No description available |
25545 | | |
25546 | | Chain information for copy of COPI_cyto_J4026_model H #48.8 |
25547 | | --- |
25548 | | Chain | Description |
25549 | | H | No description available |
25550 | | |
25551 | | Chain information for copy of COPI_cyto_J4026_model I #48.9 |
25552 | | --- |
25553 | | Chain | Description |
25554 | | I | No description available |
25555 | | |
25556 | | Chain information for copy of COPI_cyto_J4026_model J #48.10 |
25557 | | --- |
25558 | | Chain | Description |
25559 | | J | No description available |
25560 | | |
25561 | | Chain information for copy of COPI_cyto_J4026_model K #48.11 |
25562 | | --- |
25563 | | Chain | Description |
25564 | | K | No description available |
25565 | | |
25566 | | Chain information for copy of COPI_cyto_J4026_model L #48.12 |
25567 | | --- |
25568 | | Chain | Description |
25569 | | L | No description available |
25570 | | |
25571 | | Chain information for copy of COPI_cyto_J4026_model M #48.13 |
25572 | | --- |
25573 | | Chain | Description |
25574 | | M | No description available |
25575 | | |
25576 | | Chain information for copy of COPI_cyto_J4026_model N #48.14 |
25577 | | --- |
25578 | | Chain | Description |
25579 | | N | No description available |
25580 | | |
25581 | | Chain information for copy of COPI_cyto_J4026_model O #48.15 |
25582 | | --- |
25583 | | Chain | Description |
25584 | | O | No description available |
25585 | | |
25586 | | |
25587 | | > show #!46 models |
25588 | | |
25589 | | > fitmap #48.1 inMap #46 |
25590 | | |
25591 | | Fit molecule copy of COPI_cyto_J4026_model A (#48.1) to map |
25592 | | composite_map_J4021_J4026.ccp4 (#46) using 2590 atoms |
25593 | | average map value = 8.012, steps = 44 |
25594 | | shifted from previous position = 0.024 |
25595 | | rotated from previous position = 0.0413 degrees |
25596 | | atoms outside contour = 449, contour level = 4.3561 |
25597 | | |
25598 | | Position of copy of COPI_cyto_J4026_model A (#48.1) relative to |
25599 | | composite_map_J4021_J4026.ccp4 (#46) coordinates: |
25600 | | Matrix rotation and translation |
25601 | | -0.42161671 -0.88866766 -0.18030295 281.35767523 |
25602 | | -0.78137178 0.25516130 0.56951811 159.89900138 |
25603 | | -0.46010598 0.38100198 -0.80196009 237.46670115 |
25604 | | Axis -0.53247943 0.79032688 0.30306613 |
25605 | | Axis point 207.11724288 0.00000000 70.73686916 |
25606 | | Rotation angle (degrees) 169.80392724 |
25607 | | Shift along axis 48.52341756 |
25608 | | |
25609 | | |
25610 | | > fitmap #48.2 inMap #46 |
25611 | | |
25612 | | Fit molecule copy of COPI_cyto_J4026_model B (#48.2) to map |
25613 | | composite_map_J4021_J4026.ccp4 (#46) using 2426 atoms |
25614 | | average map value = 7.643, steps = 48 |
25615 | | shifted from previous position = 0.0352 |
25616 | | rotated from previous position = 0.149 degrees |
25617 | | atoms outside contour = 405, contour level = 4.3561 |
25618 | | |
25619 | | Position of copy of COPI_cyto_J4026_model B (#48.2) relative to |
25620 | | composite_map_J4021_J4026.ccp4 (#46) coordinates: |
25621 | | Matrix rotation and translation |
25622 | | -0.42061793 -0.88953902 -0.17832811 281.05427064 |
25623 | | -0.78226434 0.25604617 0.56789336 160.17141808 |
25624 | | -0.45950307 0.37836585 -0.80355226 237.63647217 |
25625 | | Axis -0.53290254 0.79059154 0.30162873 |
25626 | | Axis point 207.14962646 0.00000000 70.86650714 |
25627 | | Rotation angle (degrees) 169.75686245 |
25628 | | Shift along axis 48.53362146 |
25629 | | |
25630 | | |
25631 | | > fitmap #48.3 inMap #46 |
25632 | | |
25633 | | Fit molecule copy of COPI_cyto_J4026_model C (#48.3) to map |
25634 | | composite_map_J4021_J4026.ccp4 (#46) using 1612 atoms |
25635 | | average map value = 6.339, steps = 64 |
25636 | | shifted from previous position = 0.137 |
25637 | | rotated from previous position = 1.31 degrees |
25638 | | atoms outside contour = 282, contour level = 4.3561 |
25639 | | |
25640 | | Position of copy of COPI_cyto_J4026_model C (#48.3) relative to |
25641 | | composite_map_J4021_J4026.ccp4 (#46) coordinates: |
25642 | | Matrix rotation and translation |
25643 | | -0.43767621 -0.88321497 -0.16843654 281.87335410 |
25644 | | -0.76783552 0.26967498 0.58112309 157.06406865 |
25645 | | -0.46783349 0.38367530 -0.79619414 237.77730124 |
25646 | | Axis -0.52408907 0.79469449 0.30625367 |
25647 | | Axis point 206.03117664 0.00000000 70.20592740 |
25648 | | Rotation angle (degrees) 169.14218802 |
25649 | | Shift along axis 49.91138003 |
25650 | | |
25651 | | |
25652 | | > fitmap #48.4 inMap #46 |
25653 | | |
25654 | | Fit molecule copy of COPI_cyto_J4026_model D (#48.4) to map |
25655 | | composite_map_J4021_J4026.ccp4 (#46) using 4745 atoms |
25656 | | average map value = 6.217, steps = 64 |
25657 | | shifted from previous position = 0.346 |
25658 | | rotated from previous position = 0.851 degrees |
25659 | | atoms outside contour = 894, contour level = 4.3561 |
25660 | | |
25661 | | Position of copy of COPI_cyto_J4026_model D (#48.4) relative to |
25662 | | composite_map_J4021_J4026.ccp4 (#46) coordinates: |
25663 | | Matrix rotation and translation |
25664 | | -0.42924156 -0.88289723 -0.19037904 282.64245635 |
25665 | | -0.77884347 0.25509758 0.57299920 159.11506089 |
25666 | | -0.45733417 0.39423054 -0.79713722 235.42666761 |
25667 | | Axis -0.52934994 0.79047793 0.30811245 |
25668 | | Axis point 206.45398809 0.00000000 70.11358015 |
25669 | | Rotation angle (degrees) 170.27864285 |
25670 | | Shift along axis 48.69806493 |
25671 | | |
25672 | | |
25673 | | > fitmap #48.5 inMap #46 |
25674 | | |
25675 | | Fit molecule copy of COPI_cyto_J4026_model E (#48.5) to map |
25676 | | composite_map_J4021_J4026.ccp4 (#46) using 1876 atoms |
25677 | | average map value = 6.728, steps = 28 |
25678 | | shifted from previous position = 0.0537 |
25679 | | rotated from previous position = 0.205 degrees |
25680 | | atoms outside contour = 414, contour level = 4.3561 |
25681 | | |
25682 | | Position of copy of COPI_cyto_J4026_model E (#48.5) relative to |
25683 | | composite_map_J4021_J4026.ccp4 (#46) coordinates: |
25684 | | Matrix rotation and translation |
25685 | | -0.41906414 -0.89016033 -0.17888502 280.98895035 |
25686 | | -0.78296435 0.25453348 0.56760863 160.29649800 |
25687 | | -0.45973045 0.37792501 -0.80362965 237.75201321 |
25688 | | Axis -0.53364295 0.79011139 0.30157785 |
25689 | | Axis point 207.27636606 0.00000000 70.89197237 |
25690 | | Rotation angle (degrees) 169.76271203 |
25691 | | Shift along axis 48.40505872 |
25692 | | |
25693 | | |
25694 | | > fitmap #48.6 inMap #46 |
25695 | | |
25696 | | Fit molecule copy of COPI_cyto_J4026_model F (#48.6) to map |
25697 | | composite_map_J4021_J4026.ccp4 (#46) using 2400 atoms |
25698 | | average map value = 4.933, steps = 60 |
25699 | | shifted from previous position = 4.71 |
25700 | | rotated from previous position = 7.6 degrees |
25701 | | atoms outside contour = 678, contour level = 4.3561 |
25702 | | |
25703 | | Position of copy of COPI_cyto_J4026_model F (#48.6) relative to |
25704 | | composite_map_J4021_J4026.ccp4 (#46) coordinates: |
25705 | | Matrix rotation and translation |
25706 | | -0.53669929 -0.82504557 -0.17678715 281.80609510 |
25707 | | -0.72189432 0.34051286 0.60242808 144.12665157 |
25708 | | -0.43683232 0.45094436 -0.77834872 227.64701078 |
25709 | | Axis -0.47615916 0.81740061 0.32423556 |
25710 | | Axis point 193.11631392 -0.00000000 70.10143524 |
25711 | | Rotation angle (degrees) 170.84717448 |
25712 | | Shift along axis 57.43591697 |
25713 | | |
25714 | | |
25715 | | > fitmap #48.7 inMap #46 |
25716 | | |
25717 | | Fit molecule copy of COPI_cyto_J4026_model G (#48.7) to map |
25718 | | composite_map_J4021_J4026.ccp4 (#46) using 2302 atoms |
25719 | | average map value = 5.406, steps = 80 |
25720 | | shifted from previous position = 4.73 |
25721 | | rotated from previous position = 4.21 degrees |
25722 | | atoms outside contour = 580, contour level = 4.3561 |
25723 | | |
25724 | | Position of copy of COPI_cyto_J4026_model G (#48.7) relative to |
25725 | | composite_map_J4021_J4026.ccp4 (#46) coordinates: |
25726 | | Matrix rotation and translation |
25727 | | -0.48330635 -0.85702087 -0.17869027 283.39251275 |
25728 | | -0.74444396 0.29492363 0.59901858 152.16224396 |
25729 | | -0.46067144 0.42253437 -0.78054247 232.42661347 |
25730 | | Axis -0.50253361 0.80293313 0.32055945 |
25731 | | Axis point 201.16168457 -0.00000000 68.72114991 |
25732 | | Rotation angle (degrees) 169.88674773 |
25733 | | Shift along axis 54.26839065 |
25734 | | |
25735 | | |
25736 | | > fitmap #48.8 inMap #46 |
25737 | | |
25738 | | Fit molecule copy of COPI_cyto_J4026_model H (#48.8) to map |
25739 | | composite_map_J4021_J4026.ccp4 (#46) using 420 atoms |
25740 | | average map value = 7.082, steps = 56 |
25741 | | shifted from previous position = 3.46 |
25742 | | rotated from previous position = 2.58 degrees |
25743 | | atoms outside contour = 93, contour level = 4.3561 |
25744 | | |
25745 | | Position of copy of COPI_cyto_J4026_model H (#48.8) relative to |
25746 | | composite_map_J4021_J4026.ccp4 (#46) coordinates: |
25747 | | Matrix rotation and translation |
25748 | | -0.46181347 -0.87023765 -0.17150736 282.95432862 |
25749 | | -0.76161078 0.28994955 0.57955009 156.07549407 |
25750 | | -0.45461782 0.39826589 -0.79668497 236.09755101 |
25751 | | Axis -0.51312841 0.80134959 0.30747044 |
25752 | | Axis point 203.66513828 0.00000000 70.89396293 |
25753 | | Rotation angle (degrees) 169.82553836 |
25754 | | Shift along axis 52.47214622 |
25755 | | |
25756 | | |
25757 | | > fitmap #48.9 inMap #46 |
25758 | | |
25759 | | Fit molecule copy of COPI_cyto_J4026_model I (#48.9) to map |
25760 | | composite_map_J4021_J4026.ccp4 (#46) using 1573 atoms |
25761 | | average map value = 5.892, steps = 72 |
25762 | | shifted from previous position = 1.08 |
25763 | | rotated from previous position = 2.82 degrees |
25764 | | atoms outside contour = 307, contour level = 4.3561 |
25765 | | |
25766 | | Position of copy of COPI_cyto_J4026_model I (#48.9) relative to |
25767 | | composite_map_J4021_J4026.ccp4 (#46) coordinates: |
25768 | | Matrix rotation and translation |
25769 | | -0.44042374 -0.87092293 -0.21799127 283.13278743 |
25770 | | -0.77489855 0.24614258 0.58219077 160.68330197 |
25771 | | -0.45338635 0.42533175 -0.78328394 232.61931758 |
25772 | | Axis -0.52509607 0.78800081 0.32144805 |
25773 | | Axis point 205.38082265 0.00000000 68.72443099 |
25774 | | Rotation angle (degrees) 171.41003245 |
25775 | | Shift along axis 52.72168395 |
25776 | | |
25777 | | |
25778 | | > fitmap #48.10 inMap #46 |
25779 | | |
25780 | | Fit molecule copy of COPI_cyto_J4026_model J (#48.10) to map |
25781 | | composite_map_J4021_J4026.ccp4 (#46) using 1112 atoms |
25782 | | average map value = 6.367, steps = 68 |
25783 | | shifted from previous position = 0.853 |
25784 | | rotated from previous position = 2.18 degrees |
25785 | | atoms outside contour = 324, contour level = 4.3561 |
25786 | | |
25787 | | Position of copy of COPI_cyto_J4026_model J (#48.10) relative to |
25788 | | composite_map_J4021_J4026.ccp4 (#46) coordinates: |
25789 | | Matrix rotation and translation |
25790 | | -0.39606742 -0.89516479 -0.20447643 281.46766551 |
25791 | | -0.80125621 0.22817550 0.55310438 165.26116045 |
25792 | | -0.44846305 0.38290463 -0.80762922 236.59702773 |
25793 | | Axis -0.54558979 0.78211990 0.30103197 |
25794 | | Axis point 209.53544999 0.00000000 71.09106362 |
25795 | | Rotation angle (degrees) 171.02649245 |
25796 | | Shift along axis 46.91142523 |
25797 | | |
25798 | | |
25799 | | > fitmap #48.12 inMap #46 |
25800 | | |
25801 | | Fit molecule copy of COPI_cyto_J4026_model L (#48.12) to map |
25802 | | composite_map_J4021_J4026.ccp4 (#46) using 1420 atoms |
25803 | | average map value = 6, steps = 48 |
25804 | | shifted from previous position = 0.00534 |
25805 | | rotated from previous position = 0.0308 degrees |
25806 | | atoms outside contour = 490, contour level = 4.3561 |
25807 | | |
25808 | | Position of copy of COPI_cyto_J4026_model L (#48.12) relative to |
25809 | | composite_map_J4021_J4026.ccp4 (#46) coordinates: |
25810 | | Matrix rotation and translation |
25811 | | -0.42201385 -0.88851995 -0.18010170 281.36850791 |
25812 | | -0.78123838 0.25562354 0.56949383 159.86937778 |
25813 | | -0.45996839 0.38103664 -0.80202255 237.46846236 |
25814 | | Axis -0.53229101 0.79047409 0.30301320 |
25815 | | Axis point 207.08142201 0.00000000 70.75583573 |
25816 | | Rotation angle (degrees) 169.80350204 |
25817 | | Shift along axis 48.55875296 |
25818 | | |
25819 | | |
25820 | | > fitmap #48.13 inMap #46 |
25821 | | |
25822 | | Fit molecule copy of COPI_cyto_J4026_model M (#48.13) to map |
25823 | | composite_map_J4021_J4026.ccp4 (#46) using 2567 atoms |
25824 | | average map value = 6.934, steps = 48 |
25825 | | shifted from previous position = 0.0327 |
25826 | | rotated from previous position = 0.0247 degrees |
25827 | | atoms outside contour = 577, contour level = 4.3561 |
25828 | | |
25829 | | Position of copy of COPI_cyto_J4026_model M (#48.13) relative to |
25830 | | composite_map_J4021_J4026.ccp4 (#46) coordinates: |
25831 | | Matrix rotation and translation |
25832 | | -0.42198871 -0.88852999 -0.18011109 281.34034070 |
25833 | | -0.78150296 0.25580865 0.56904751 159.96958762 |
25834 | | -0.45954180 0.38088898 -0.80233717 237.44017007 |
25835 | | Axis -0.53232061 0.79053934 0.30279089 |
25836 | | Axis point 207.07606122 0.00000000 70.77828437 |
25837 | | Rotation angle (degrees) 169.82040522 |
25838 | | Shift along axis 48.59371022 |
25839 | | |
25840 | | |
25841 | | > fitmap #48.14 inMap #46 |
25842 | | |
25843 | | Fit molecule copy of COPI_cyto_J4026_model N (#48.14) to map |
25844 | | composite_map_J4021_J4026.ccp4 (#46) using 930 atoms |
25845 | | average map value = 7.254, steps = 40 |
25846 | | shifted from previous position = 0.0472 |
25847 | | rotated from previous position = 0.247 degrees |
25848 | | atoms outside contour = 214, contour level = 4.3561 |
25849 | | |
25850 | | Position of copy of COPI_cyto_J4026_model N (#48.14) relative to |
25851 | | composite_map_J4021_J4026.ccp4 (#46) coordinates: |
25852 | | Matrix rotation and translation |
25853 | | -0.42294225 -0.88896586 -0.17566891 281.09587079 |
25854 | | -0.78004341 0.25851684 0.56982571 159.40297655 |
25855 | | -0.46114222 0.37803274 -0.80276965 237.94268675 |
25856 | | Axis -0.53164432 0.79132341 0.30192975 |
25857 | | Axis point 206.92420625 0.00000000 70.92756128 |
25858 | | Rotation angle (degrees) 169.60827201 |
25859 | | Shift along axis 48.53825925 |
25860 | | |
25861 | | |
25862 | | > fitmap #48.15 inMap #46 |
25863 | | |
25864 | | Fit molecule copy of COPI_cyto_J4026_model O (#48.15) to map |
25865 | | composite_map_J4021_J4026.ccp4 (#46) using 1064 atoms |
25866 | | average map value = 6.216, steps = 60 |
25867 | | shifted from previous position = 0.0698 |
25868 | | rotated from previous position = 0.468 degrees |
25869 | | atoms outside contour = 130, contour level = 4.3561 |
25870 | | |
25871 | | Position of copy of COPI_cyto_J4026_model O (#48.15) relative to |
25872 | | composite_map_J4021_J4026.ccp4 (#46) coordinates: |
25873 | | Matrix rotation and translation |
25874 | | -0.41919869 -0.88836490 -0.18729731 281.44552791 |
25875 | | -0.78315395 0.24946223 0.56959504 160.73613068 |
25876 | | -0.45928463 0.38545612 -0.80030071 236.81019199 |
25877 | | Axis -0.53389619 0.78860565 0.30505080 |
25878 | | Axis point 207.35794189 0.00000000 70.38133823 |
25879 | | Rotation angle (degrees) 170.06980434 |
25880 | | Shift along axis 48.73386479 |
25881 | | |
25882 | | |
25883 | | > color #48.13 gammav3 |
25884 | | |
25885 | | > color #48.14 gammav3 |
25886 | | |
25887 | | > color #48.15 gammav3 |
25888 | | |
25889 | | > combine #48.1 #48.2 #48.3 #48.4 #48.5 #48.6 #48.7 #48.8 #48.9 #48.10 #48.11 |
25890 | | > #48.12 #48.13 #48.14 #48.15 |
25891 | | |
25892 | | > rename #49 Model_for_composite_J4021_J4026 |
25893 | | |
25894 | | > hide #!49 models |
25895 | | |
25896 | | > hide #!46 models |
25897 | | |
25898 | | > show #!47 models |
25899 | | |
25900 | | > close #48 |
25901 | | |
25902 | | > combine #41 |
25903 | | |
25904 | | > split #48 chains |
25905 | | |
25906 | | Split copy of COPI_cyto_J4027_model (#48) into 15 models |
25907 | | Chain information for copy of COPI_cyto_J4027_model A #48.1 |
25908 | | --- |
25909 | | Chain | Description |
25910 | | A | No description available |
25911 | | |
25912 | | Chain information for copy of COPI_cyto_J4027_model B #48.2 |
25913 | | --- |
25914 | | Chain | Description |
25915 | | B | No description available |
25916 | | |
25917 | | Chain information for copy of COPI_cyto_J4027_model C #48.3 |
25918 | | --- |
25919 | | Chain | Description |
25920 | | C | No description available |
25921 | | |
25922 | | Chain information for copy of COPI_cyto_J4027_model D #48.4 |
25923 | | --- |
25924 | | Chain | Description |
25925 | | D | No description available |
25926 | | |
25927 | | Chain information for copy of COPI_cyto_J4027_model E #48.5 |
25928 | | --- |
25929 | | Chain | Description |
25930 | | E | No description available |
25931 | | |
25932 | | Chain information for copy of COPI_cyto_J4027_model F #48.6 |
25933 | | --- |
25934 | | Chain | Description |
25935 | | F | No description available |
25936 | | |
25937 | | Chain information for copy of COPI_cyto_J4027_model G #48.7 |
25938 | | --- |
25939 | | Chain | Description |
25940 | | G | No description available |
25941 | | |
25942 | | Chain information for copy of COPI_cyto_J4027_model H #48.8 |
25943 | | --- |
25944 | | Chain | Description |
25945 | | H | No description available |
25946 | | |
25947 | | Chain information for copy of COPI_cyto_J4027_model I #48.9 |
25948 | | --- |
25949 | | Chain | Description |
25950 | | I | No description available |
25951 | | |
25952 | | Chain information for copy of COPI_cyto_J4027_model J #48.10 |
25953 | | --- |
25954 | | Chain | Description |
25955 | | J | No description available |
25956 | | |
25957 | | Chain information for copy of COPI_cyto_J4027_model K #48.11 |
25958 | | --- |
25959 | | Chain | Description |
25960 | | K | No description available |
25961 | | |
25962 | | Chain information for copy of COPI_cyto_J4027_model L #48.12 |
25963 | | --- |
25964 | | Chain | Description |
25965 | | L | No description available |
25966 | | |
25967 | | Chain information for copy of COPI_cyto_J4027_model M #48.13 |
25968 | | --- |
25969 | | Chain | Description |
25970 | | M | No description available |
25971 | | |
25972 | | Chain information for copy of COPI_cyto_J4027_model N #48.14 |
25973 | | --- |
25974 | | Chain | Description |
25975 | | N | No description available |
25976 | | |
25977 | | Chain information for copy of COPI_cyto_J4027_model O #48.15 |
25978 | | --- |
25979 | | Chain | Description |
25980 | | O | No description available |
25981 | | |
25982 | | |
25983 | | > color #48.13 gammav3 |
25984 | | |
25985 | | > color #48.14 gammav3 |
25986 | | |
25987 | | > color #48.15 gammav3 |
25988 | | |
25989 | | > fitmap #48.15 inMap #46 |
25990 | | |
25991 | | Fit molecule copy of COPI_cyto_J4027_model O (#48.15) to map |
25992 | | composite_map_J4021_J4026.ccp4 (#46) using 1064 atoms |
25993 | | average map value = 6.216, steps = 68 |
25994 | | shifted from previous position = 0.926 |
25995 | | rotated from previous position = 3.78 degrees |
25996 | | atoms outside contour = 131, contour level = 4.3561 |
25997 | | |
25998 | | Position of copy of COPI_cyto_J4027_model O (#48.15) relative to |
25999 | | composite_map_J4021_J4026.ccp4 (#46) coordinates: |
26000 | | Matrix rotation and translation |
26001 | | -0.34404909 -0.93260747 -0.10896575 269.91254838 |
26002 | | -0.80984309 0.23600764 0.53707968 166.69850821 |
26003 | | -0.47516777 0.27302693 -0.83646393 251.15790315 |
26004 | | Axis -0.56437651 0.78270657 0.26239203 |
26005 | | Axis point 213.21055464 0.00000000 75.30227951 |
26006 | | Rotation angle (degrees) 166.47125621 |
26007 | | Shift along axis 44.04554723 |
26008 | | |
26009 | | |
26010 | | > fitmap #48.14 inMap #46 |
26011 | | |
26012 | | Fit molecule copy of COPI_cyto_J4027_model N (#48.14) to map |
26013 | | composite_map_J4021_J4026.ccp4 (#46) using 930 atoms |
26014 | | average map value = 7.254, steps = 68 |
26015 | | shifted from previous position = 4.09 |
26016 | | rotated from previous position = 12.1 degrees |
26017 | | atoms outside contour = 214, contour level = 4.3561 |
26018 | | |
26019 | | Position of copy of COPI_cyto_J4027_model N (#48.14) relative to |
26020 | | composite_map_J4021_J4026.ccp4 (#46) coordinates: |
26021 | | Matrix rotation and translation |
26022 | | -0.29521822 -0.95538003 0.00975828 254.00941556 |
26023 | | -0.83564878 0.26314499 0.48212638 171.86630354 |
26024 | | -0.46318175 0.13417799 -0.87604735 264.92556241 |
26025 | | Axis -0.58065970 0.78924699 0.19980868 |
26026 | | Axis point 215.16338827 0.00000000 83.71664964 |
26027 | | Rotation angle (degrees) 162.56553634 |
26028 | | Shift along axis 41.08635962 |
26029 | | |
26030 | | |
26031 | | > fitmap #48.13 inMap #46 |
26032 | | |
26033 | | Fit molecule copy of COPI_cyto_J4027_model M (#48.13) to map |
26034 | | composite_map_J4021_J4026.ccp4 (#46) using 2567 atoms |
26035 | | average map value = 6.934, steps = 184 |
26036 | | shifted from previous position = 24.3 |
26037 | | rotated from previous position = 38.5 degrees |
26038 | | atoms outside contour = 574, contour level = 4.3561 |
26039 | | |
26040 | | Position of copy of COPI_cyto_J4027_model M (#48.13) relative to |
26041 | | composite_map_J4021_J4026.ccp4 (#46) coordinates: |
26042 | | Matrix rotation and translation |
26043 | | -0.06511231 -0.96101824 0.26870867 188.55069019 |
26044 | | -0.97216064 0.12183140 0.20015198 237.48407748 |
26045 | | -0.22508686 -0.24819563 -0.94219682 265.26082374 |
26046 | | Axis -0.67212131 0.74025262 -0.01670366 |
26047 | | Axis point 232.93504959 0.00000000 109.96611159 |
26048 | | Rotation angle (degrees) 160.51672010 |
26049 | | Shift along axis 44.63844706 |
26050 | | |
26051 | | |
26052 | | > fitmap #48.13 inMap #47 |
26053 | | |
26054 | | Fit molecule copy of COPI_cyto_J4027_model M (#48.13) to map |
26055 | | J4021_J4027_composite_map.ccp4 (#47) using 2567 atoms |
26056 | | average map value = 5.305, steps = 140 |
26057 | | shifted from previous position = 20.1 |
26058 | | rotated from previous position = 23.5 degrees |
26059 | | atoms outside contour = 1497, contour level = 5.8074 |
26060 | | |
26061 | | Position of copy of COPI_cyto_J4027_model M (#48.13) relative to |
26062 | | J4021_J4027_composite_map.ccp4 (#47) coordinates: |
26063 | | Matrix rotation and translation |
26064 | | -0.20584357 -0.97794249 -0.03545317 247.21508377 |
26065 | | -0.89336671 0.17300799 0.41468563 196.10217944 |
26066 | | -0.39940501 0.11703305 -0.90927384 267.43384909 |
26067 | | Axis -0.62307640 0.76186070 0.17704255 |
26068 | | Axis point 224.46487691 0.00000000 89.15375612 |
26069 | | Rotation angle (degrees) 166.18089851 |
26070 | | Shift along axis 42.71583076 |
26071 | | |
26072 | | |
26073 | | > close #48 |
26074 | | |
26075 | | > combine #41 |
26076 | | |
26077 | | > split #48 chains |
26078 | | |
26079 | | Split copy of COPI_cyto_J4027_model (#48) into 15 models |
26080 | | Chain information for copy of COPI_cyto_J4027_model A #48.1 |
26081 | | --- |
26082 | | Chain | Description |
26083 | | A | No description available |
26084 | | |
26085 | | Chain information for copy of COPI_cyto_J4027_model B #48.2 |
26086 | | --- |
26087 | | Chain | Description |
26088 | | B | No description available |
26089 | | |
26090 | | Chain information for copy of COPI_cyto_J4027_model C #48.3 |
26091 | | --- |
26092 | | Chain | Description |
26093 | | C | No description available |
26094 | | |
26095 | | Chain information for copy of COPI_cyto_J4027_model D #48.4 |
26096 | | --- |
26097 | | Chain | Description |
26098 | | D | No description available |
26099 | | |
26100 | | Chain information for copy of COPI_cyto_J4027_model E #48.5 |
26101 | | --- |
26102 | | Chain | Description |
26103 | | E | No description available |
26104 | | |
26105 | | Chain information for copy of COPI_cyto_J4027_model F #48.6 |
26106 | | --- |
26107 | | Chain | Description |
26108 | | F | No description available |
26109 | | |
26110 | | Chain information for copy of COPI_cyto_J4027_model G #48.7 |
26111 | | --- |
26112 | | Chain | Description |
26113 | | G | No description available |
26114 | | |
26115 | | Chain information for copy of COPI_cyto_J4027_model H #48.8 |
26116 | | --- |
26117 | | Chain | Description |
26118 | | H | No description available |
26119 | | |
26120 | | Chain information for copy of COPI_cyto_J4027_model I #48.9 |
26121 | | --- |
26122 | | Chain | Description |
26123 | | I | No description available |
26124 | | |
26125 | | Chain information for copy of COPI_cyto_J4027_model J #48.10 |
26126 | | --- |
26127 | | Chain | Description |
26128 | | J | No description available |
26129 | | |
26130 | | Chain information for copy of COPI_cyto_J4027_model K #48.11 |
26131 | | --- |
26132 | | Chain | Description |
26133 | | K | No description available |
26134 | | |
26135 | | Chain information for copy of COPI_cyto_J4027_model L #48.12 |
26136 | | --- |
26137 | | Chain | Description |
26138 | | L | No description available |
26139 | | |
26140 | | Chain information for copy of COPI_cyto_J4027_model M #48.13 |
26141 | | --- |
26142 | | Chain | Description |
26143 | | M | No description available |
26144 | | |
26145 | | Chain information for copy of COPI_cyto_J4027_model N #48.14 |
26146 | | --- |
26147 | | Chain | Description |
26148 | | N | No description available |
26149 | | |
26150 | | Chain information for copy of COPI_cyto_J4027_model O #48.15 |
26151 | | --- |
26152 | | Chain | Description |
26153 | | O | No description available |
26154 | | |
26155 | | |
26156 | | > color #48.13 gammav3 |
26157 | | |
26158 | | > color #48.14 gammav3 |
26159 | | |
26160 | | > color #48.15 gammav3 |
26161 | | |
26162 | | > fitmap #48.15 inMap #47 |
26163 | | |
26164 | | Fit molecule copy of COPI_cyto_J4027_model O (#48.15) to map |
26165 | | J4021_J4027_composite_map.ccp4 (#47) using 1064 atoms |
26166 | | average map value = 7.175, steps = 80 |
26167 | | shifted from previous position = 0.0377 |
26168 | | rotated from previous position = 0.0512 degrees |
26169 | | atoms outside contour = 362, contour level = 5.8074 |
26170 | | |
26171 | | Position of copy of COPI_cyto_J4027_model O (#48.15) relative to |
26172 | | J4021_J4027_composite_map.ccp4 (#47) coordinates: |
26173 | | Matrix rotation and translation |
26174 | | -0.36445970 -0.91742956 -0.15966261 274.93147533 |
26175 | | -0.79741803 0.21892271 0.56231428 164.22520611 |
26176 | | -0.48092997 0.33225874 -0.81136337 243.41623113 |
26177 | | Axis -0.55708089 0.77795083 0.29060864 |
26178 | | Axis point 211.70950704 0.00000000 70.89847596 |
26179 | | Rotation angle (degrees) 168.08366956 |
26180 | | Shift along axis 45.33892553 |
26181 | | |
26182 | | |
26183 | | > fitmap #48.14 inMap #47 |
26184 | | |
26185 | | Fit molecule copy of COPI_cyto_J4027_model N (#48.14) to map |
26186 | | J4021_J4027_composite_map.ccp4 (#47) using 930 atoms |
26187 | | average map value = 7.843, steps = 48 |
26188 | | shifted from previous position = 0.0452 |
26189 | | rotated from previous position = 0.236 degrees |
26190 | | atoms outside contour = 308, contour level = 5.8074 |
26191 | | |
26192 | | Position of copy of COPI_cyto_J4027_model N (#48.14) relative to |
26193 | | J4021_J4027_composite_map.ccp4 (#47) coordinates: |
26194 | | Matrix rotation and translation |
26195 | | -0.36749036 -0.91663154 -0.15728153 275.08372435 |
26196 | | -0.79611294 0.22261900 0.56271217 163.59058237 |
26197 | | -0.48078586 0.33200516 -0.81155255 243.40917516 |
26198 | | Axis -0.55562941 0.77912031 0.29025419 |
26199 | | Axis point 211.38295724 0.00000000 71.01177343 |
26200 | | Rotation angle (degrees) 168.01774651 |
26201 | | Shift along axis 45.26267005 |
26202 | | |
26203 | | |
26204 | | > fitmap #48.13 inMap #47 |
26205 | | |
26206 | | Fit molecule copy of COPI_cyto_J4027_model M (#48.13) to map |
26207 | | J4021_J4027_composite_map.ccp4 (#47) using 2567 atoms |
26208 | | average map value = 6.181, steps = 48 |
26209 | | shifted from previous position = 0.0646 |
26210 | | rotated from previous position = 0.137 degrees |
26211 | | atoms outside contour = 1105, contour level = 5.8074 |
26212 | | |
26213 | | Position of copy of COPI_cyto_J4027_model M (#48.13) relative to |
26214 | | J4021_J4027_composite_map.ccp4 (#47) coordinates: |
26215 | | Matrix rotation and translation |
26216 | | -0.36546244 -0.91673285 -0.16136328 275.21816301 |
26217 | | -0.79822949 0.21948305 0.56094285 164.44314904 |
26218 | | -0.47881823 0.33380847 -0.81197600 242.98792253 |
26219 | | Axis -0.55679103 0.77820041 0.29049589 |
26220 | | Axis point 211.65663706 0.00000000 70.90505517 |
26221 | | Rotation angle (degrees) 168.23094482 |
26222 | | Shift along axis 45.31771373 |
26223 | | |
26224 | | |
26225 | | > fitmap #48.12 inMap #47 |
26226 | | |
26227 | | Fit molecule copy of COPI_cyto_J4027_model L (#48.12) to map |
26228 | | J4021_J4027_composite_map.ccp4 (#47) using 1420 atoms |
26229 | | average map value = 5.269, steps = 64 |
26230 | | shifted from previous position = 0.0436 |
26231 | | rotated from previous position = 0.124 degrees |
26232 | | atoms outside contour = 716, contour level = 5.8074 |
26233 | | |
26234 | | Position of copy of COPI_cyto_J4027_model L (#48.12) relative to |
26235 | | J4021_J4027_composite_map.ccp4 (#47) coordinates: |
26236 | | Matrix rotation and translation |
26237 | | -0.36575950 -0.91679867 -0.16031284 275.10067764 |
26238 | | -0.79603501 0.21891045 0.56427520 163.73853261 |
26239 | | -0.48223259 0.33400364 -0.80987241 243.20437721 |
26240 | | Axis -0.55646287 0.77793538 0.29183150 |
26241 | | Axis point 211.57205619 0.00000000 70.70465662 |
26242 | | Rotation angle (degrees) 168.05887425 |
26243 | | Shift along axis 45.26938091 |
26244 | | |
26245 | | |
26246 | | > fitmap #48.11 inMap #47 |
26247 | | |
26248 | | Fit molecule copy of COPI_cyto_J4027_model K (#48.11) to map |
26249 | | J4021_J4027_composite_map.ccp4 (#47) using 319 atoms |
26250 | | average map value = 6.544, steps = 680 |
26251 | | shifted from previous position = 34.4 |
26252 | | rotated from previous position = 55.2 degrees |
26253 | | atoms outside contour = 122, contour level = 5.8074 |
26254 | | |
26255 | | Position of copy of COPI_cyto_J4027_model K (#48.11) relative to |
26256 | | J4021_J4027_composite_map.ccp4 (#47) coordinates: |
26257 | | Matrix rotation and translation |
26258 | | 0.24248330 -0.95058432 0.19388478 218.57357673 |
26259 | | -0.33056267 0.10693534 0.93770633 73.53913137 |
26260 | | -0.91210207 -0.29146919 -0.28829766 267.39969287 |
26261 | | Axis -0.69605025 0.62629170 0.35110220 |
26262 | | Axis point 0.00000000 252.37218567 153.78684989 |
26263 | | Rotation angle (degrees) 117.99791989 |
26264 | | Shift along axis -12.19662658 |
26265 | | |
26266 | | |
26267 | | > ui tool show Matchmaker |
26268 | | |
26269 | | > matchmaker #48.11 to #49 |
26270 | | |
26271 | | Parameters |
26272 | | --- |
26273 | | Chain pairing | bb |
26274 | | Alignment algorithm | Needleman-Wunsch |
26275 | | Similarity matrix | BLOSUM-62 |
26276 | | SS fraction | 0.3 |
26277 | | Gap open (HH/SS/other) | 18/18/6 |
26278 | | Gap extend | 1 |
26279 | | SS matrix | | | H | S | O |
26280 | | ---|---|---|--- |
26281 | | H | 6 | -9 | -6 |
26282 | | S | | 6 | -6 |
26283 | | O | | | 4 |
26284 | | Iteration cutoff | 2 |
26285 | | |
26286 | | Matchmaker Model_for_composite_J4021_J4026, chain K (#49) with copy of |
26287 | | COPI_cyto_J4027_model K, chain K (#48.11), sequence alignment score = 192.1 |
26288 | | RMSD between 37 pruned atom pairs is 0.000 angstroms; (across all 37 pairs: |
26289 | | 0.000) |
26290 | | |
26291 | | |
26292 | | > fitmap #48.10 inMap #47 |
26293 | | |
26294 | | Fit molecule copy of COPI_cyto_J4027_model J (#48.10) to map |
26295 | | J4021_J4027_composite_map.ccp4 (#47) using 1112 atoms |
26296 | | average map value = 6.595, steps = 68 |
26297 | | shifted from previous position = 0.392 |
26298 | | rotated from previous position = 3.79 degrees |
26299 | | atoms outside contour = 449, contour level = 5.8074 |
26300 | | |
26301 | | Position of copy of COPI_cyto_J4027_model J (#48.10) relative to |
26302 | | J4021_J4027_composite_map.ccp4 (#47) coordinates: |
26303 | | Matrix rotation and translation |
26304 | | -0.36954265 -0.92395010 -0.09876469 269.14351053 |
26305 | | -0.79489549 0.25928902 0.54855298 162.57033903 |
26306 | | -0.48122698 0.28122133 -0.83026211 249.17503135 |
26307 | | Axis -0.55216997 0.78997075 0.26656057 |
26308 | | Axis point 209.80677258 0.00000000 74.24424921 |
26309 | | Rotation angle (degrees) 165.99103601 |
26310 | | Shift along axis 46.23308621 |
26311 | | |
26312 | | |
26313 | | > fitmap #48.9 inMap #47 |
26314 | | |
26315 | | Fit molecule copy of COPI_cyto_J4027_model I (#48.9) to map |
26316 | | J4021_J4027_composite_map.ccp4 (#47) using 1573 atoms |
26317 | | average map value = 5.861, steps = 68 |
26318 | | shifted from previous position = 1.24 |
26319 | | rotated from previous position = 3.45 degrees |
26320 | | atoms outside contour = 773, contour level = 5.8074 |
26321 | | |
26322 | | Position of copy of COPI_cyto_J4027_model I (#48.9) relative to |
26323 | | J4021_J4027_composite_map.ccp4 (#47) coordinates: |
26324 | | Matrix rotation and translation |
26325 | | -0.37973756 -0.89954761 -0.21590159 277.24792433 |
26326 | | -0.80457095 0.20595800 0.55699812 167.23271540 |
26327 | | -0.45657967 0.38522125 -0.80195986 236.87971276 |
26328 | | Axis -0.55310174 0.77495569 0.30581391 |
26329 | | Axis point 209.49101626 0.00000000 70.11224809 |
26330 | | Rotation angle (degrees) 171.06667095 |
26331 | | Shift along axis 48.69274569 |
26332 | | |
26333 | | |
26334 | | > fitmap #48.8 inMap #47 |
26335 | | |
26336 | | Fit molecule copy of COPI_cyto_J4027_model H (#48.8) to map |
26337 | | J4021_J4027_composite_map.ccp4 (#47) using 420 atoms |
26338 | | average map value = 6.824, steps = 56 |
26339 | | shifted from previous position = 3.38 |
26340 | | rotated from previous position = 2.53 degrees |
26341 | | atoms outside contour = 149, contour level = 5.8074 |
26342 | | |
26343 | | Position of copy of COPI_cyto_J4027_model H (#48.8) relative to |
26344 | | J4021_J4027_composite_map.ccp4 (#47) coordinates: |
26345 | | Matrix rotation and translation |
26346 | | -0.40542761 -0.90093167 -0.15475978 276.94991852 |
26347 | | -0.78307394 0.25494593 0.56727223 160.97102962 |
26348 | | -0.47161814 0.35117617 -0.80885823 241.79873195 |
26349 | | Axis -0.53858123 0.78971345 0.29373956 |
26350 | | Axis point 207.95662584 0.00000000 71.49993787 |
26351 | | Rotation angle (degrees) 168.42701468 |
26352 | | Shift along axis 48.98681208 |
26353 | | |
26354 | | |
26355 | | > fitmap #48.7 inMap #47 |
26356 | | |
26357 | | Fit molecule copy of COPI_cyto_J4027_model G (#48.7) to map |
26358 | | J4021_J4027_composite_map.ccp4 (#47) using 2302 atoms |
26359 | | average map value = 5.298, steps = 64 |
26360 | | shifted from previous position = 4.62 |
26361 | | rotated from previous position = 4.04 degrees |
26362 | | atoms outside contour = 1402, contour level = 5.8074 |
26363 | | |
26364 | | Position of copy of COPI_cyto_J4027_model G (#48.7) relative to |
26365 | | J4021_J4027_composite_map.ccp4 (#47) coordinates: |
26366 | | Matrix rotation and translation |
26367 | | -0.42560093 -0.89008522 -0.16313234 277.69917989 |
26368 | | -0.76548490 0.25798571 0.58947118 157.21311144 |
26369 | | -0.48259377 0.37575482 -0.79114574 238.50649670 |
26370 | | Axis -0.52893684 0.79065037 0.30837932 |
26371 | | Axis point 205.81643686 0.00000000 68.93017279 |
26372 | | Rotation angle (degrees) 168.34463133 |
26373 | | Shift along axis 50.96574868 |
26374 | | |
26375 | | |
26376 | | > fitmap #48.6 inMap #47 |
26377 | | |
26378 | | Fit molecule copy of COPI_cyto_J4027_model F (#48.6) to map |
26379 | | J4021_J4027_composite_map.ccp4 (#47) using 2400 atoms |
26380 | | average map value = 4.862, steps = 56 |
26381 | | shifted from previous position = 4.53 |
26382 | | rotated from previous position = 7.12 degrees |
26383 | | atoms outside contour = 1891, contour level = 5.8074 |
26384 | | |
26385 | | Position of copy of COPI_cyto_J4027_model F (#48.6) relative to |
26386 | | J4021_J4027_composite_map.ccp4 (#47) coordinates: |
26387 | | Matrix rotation and translation |
26388 | | -0.47631791 -0.86443647 -0.16084418 276.54476513 |
26389 | | -0.74836077 0.30252539 0.59028346 150.15879842 |
26390 | | -0.46160309 0.40153206 -0.79100860 234.60647851 |
26391 | | Axis -0.50526012 0.80508798 0.31071780 |
26392 | | Axis point 198.13646970 0.00000000 70.54602612 |
26393 | | Rotation angle (degrees) 169.23470102 |
26394 | | Shift along axis 54.06041328 |
26395 | | |
26396 | | |
26397 | | > fitmap #48.5 inMap #47 |
26398 | | |
26399 | | Fit molecule copy of COPI_cyto_J4027_model E (#48.5) to map |
26400 | | J4021_J4027_composite_map.ccp4 (#47) using 1876 atoms |
26401 | | average map value = 6.882, steps = 40 |
26402 | | shifted from previous position = 0.0401 |
26403 | | rotated from previous position = 0.0808 degrees |
26404 | | atoms outside contour = 679, contour level = 5.8074 |
26405 | | |
26406 | | Position of copy of COPI_cyto_J4027_model E (#48.5) relative to |
26407 | | J4021_J4027_composite_map.ccp4 (#47) coordinates: |
26408 | | Matrix rotation and translation |
26409 | | -0.36460635 -0.91721639 -0.16055006 274.96464339 |
26410 | | -0.79803633 0.21895894 0.56142232 164.31313284 |
26411 | | -0.47979187 0.33282292 -0.81180581 243.25738221 |
26412 | | Axis -0.55710084 0.77799805 0.29044395 |
26413 | | Axis point 211.64530256 0.00000000 70.95305334 |
26414 | | Rotation angle (degrees) 168.16062068 |
26415 | | Shift along axis 45.30489541 |
26416 | | |
26417 | | |
26418 | | > fitmap #48.4 inMap #47 |
26419 | | |
26420 | | Fit molecule copy of COPI_cyto_J4027_model D (#48.4) to map |
26421 | | J4021_J4027_composite_map.ccp4 (#47) using 4745 atoms |
26422 | | average map value = 6.326, steps = 80 |
26423 | | shifted from previous position = 0.232 |
26424 | | rotated from previous position = 0.637 degrees |
26425 | | atoms outside contour = 1901, contour level = 5.8074 |
26426 | | |
26427 | | Position of copy of COPI_cyto_J4027_model D (#48.4) relative to |
26428 | | J4021_J4027_composite_map.ccp4 (#47) coordinates: |
26429 | | Matrix rotation and translation |
26430 | | -0.36403643 -0.91927407 -0.14970864 274.32296003 |
26431 | | -0.79661223 0.22402751 0.56144514 163.63243958 |
26432 | | -0.48258310 0.32364622 -0.81385913 244.76587458 |
26433 | | Axis -0.55679826 0.77941446 0.28720862 |
26434 | | Axis point 211.67256763 0.00000000 71.48567291 |
26435 | | Rotation angle (degrees) 167.67003358 |
26436 | | Shift along axis 45.09381034 |
26437 | | |
26438 | | |
26439 | | > fitmap #48.3 inMap #47 |
26440 | | |
26441 | | Fit molecule copy of COPI_cyto_J4027_model C (#48.3) to map |
26442 | | J4021_J4027_composite_map.ccp4 (#47) using 1612 atoms |
26443 | | average map value = 6.318, steps = 80 |
26444 | | shifted from previous position = 0.228 |
26445 | | rotated from previous position = 1.41 degrees |
26446 | | atoms outside contour = 653, contour level = 5.8074 |
26447 | | |
26448 | | Position of copy of COPI_cyto_J4027_model C (#48.3) relative to |
26449 | | J4021_J4027_composite_map.ccp4 (#47) coordinates: |
26450 | | Matrix rotation and translation |
26451 | | -0.38173446 -0.91224090 -0.14864510 275.71683603 |
26452 | | -0.78322797 0.23388065 0.57606754 161.04568217 |
26453 | | -0.49074715 0.33632783 -0.80377288 243.57494299 |
26454 | | Axis -0.54833896 0.78246479 0.29508175 |
26455 | | Axis point 210.53404344 0.00000000 70.11714477 |
26456 | | Rotation angle (degrees) 167.37286555 |
26457 | | Shift along axis 46.70081412 |
26458 | | |
26459 | | |
26460 | | > fitmap #48.2 inMap #47 |
26461 | | |
26462 | | Fit molecule copy of COPI_cyto_J4027_model B (#48.2) to map |
26463 | | J4021_J4027_composite_map.ccp4 (#47) using 2426 atoms |
26464 | | average map value = 7.483, steps = 48 |
26465 | | shifted from previous position = 0.0329 |
26466 | | rotated from previous position = 0.105 degrees |
26467 | | atoms outside contour = 734, contour level = 5.8074 |
26468 | | |
26469 | | Position of copy of COPI_cyto_J4027_model B (#48.2) relative to |
26470 | | J4021_J4027_composite_map.ccp4 (#47) coordinates: |
26471 | | Matrix rotation and translation |
26472 | | -0.36430973 -0.91755945 -0.15925792 274.83521303 |
26473 | | -0.79785551 0.21931761 0.56153930 164.30315268 |
26474 | | -0.48031762 0.33163904 -0.81197940 243.32909311 |
26475 | | Axis -0.55716005 0.77808367 0.29010081 |
26476 | | Axis point 211.66844489 0.00000000 70.92695219 |
26477 | | Rotation angle (degrees) 168.09354740 |
26478 | | Shift along axis 45.30436790 |
26479 | | |
26480 | | |
26481 | | > fitmap #48.1 inMap #47 |
26482 | | |
26483 | | Fit molecule copy of COPI_cyto_J4027_model A (#48.1) to map |
26484 | | J4021_J4027_composite_map.ccp4 (#47) using 2590 atoms |
26485 | | average map value = 7.767, steps = 64 |
26486 | | shifted from previous position = 0.0157 |
26487 | | rotated from previous position = 0.0377 degrees |
26488 | | atoms outside contour = 732, contour level = 5.8074 |
26489 | | |
26490 | | Position of copy of COPI_cyto_J4027_model A (#48.1) relative to |
26491 | | J4021_J4027_composite_map.ccp4 (#47) coordinates: |
26492 | | Matrix rotation and translation |
26493 | | -0.36433385 -0.91729312 -0.16073020 274.99544480 |
26494 | | -0.79752965 0.21821532 0.56243093 164.22320875 |
26495 | | -0.48084022 0.33309973 -0.81107168 243.29313210 |
26496 | | Axis -0.55718331 0.77773961 0.29097743 |
26497 | | Axis point 211.71070241 0.00000000 70.85027180 |
26498 | | Rotation angle (degrees) 168.12395214 |
26499 | | Shift along axis 45.29283319 |
26500 | | |
26501 | | |
26502 | | > combine #48.1 #48.2 #48.3 #48.4 #48.5 #48.6 #48.7 #48.8 #48.9 #48.10 #48.11 |
26503 | | > #48.12 #48.13 #48.14 #48.15 |
26504 | | |
26505 | | > rename #50 Model_for_composite_J4021_J4027 |
26506 | | |
26507 | | > close #48 |
26508 | | |
26509 | | > show #!49 models |
26510 | | |
26511 | | > hide #!49 models |
26512 | | |
26513 | | > hide #!50 models |
26514 | | |
26515 | | > save composite_model_J4021_J4026.pdb #49 |
26516 | | |
26517 | | > save composite_model_J4021_J4027.pdb #50 |
26518 | | |
26519 | | > save "/Users/becca/Desktop/Postdoc/COPI notebooks and |
26520 | | > resources/Processing/W20_MM_cyto_COPI/20250707_W20_COPI_cyto_alignments.cxs" |
26521 | | |
26522 | | > hide #!47 models |
26523 | | |
26524 | | > show #!1 models |
26525 | | |
26526 | | > color #1 #a9a9a9bf models |
26527 | | |
26528 | | > ui tool show "Surface Color" |
26529 | | |
26530 | | > ui tool show "Color Zone" |
26531 | | |
26532 | | > color zone #1 near #35 distance 6 |
26533 | | |
26534 | | > hide #!1 models |
26535 | | |
26536 | | > show #!1 models |
26537 | | |
26538 | | > select #35/K |
26539 | | |
26540 | | 319 atoms, 320 bonds, 37 residues, 1 model selected |
26541 | | |
26542 | | > hide #!1 models |
26543 | | |
26544 | | > show #!35 models |
26545 | | |
26546 | | > select #35/M |
26547 | | |
26548 | | 4552 atoms, 4624 bonds, 582 residues, 1 model selected |
26549 | | |
26550 | | > color sel gammav3 |
26551 | | |
26552 | | > select add #35 |
26553 | | |
26554 | | 27347 atoms, 27860 bonds, 1 pseudobond, 3435 residues, 2 models selected |
26555 | | |
26556 | | > hide #!35 models |
26557 | | |
26558 | | > select subtract #35 |
26559 | | |
26560 | | Nothing selected |
26561 | | |
26562 | | > show #!36 models |
26563 | | |
26564 | | > hide #!36 models |
26565 | | |
26566 | | > show #!37 models |
26567 | | |
26568 | | > select #36/M |
26569 | | |
26570 | | 4552 atoms, 4624 bonds, 582 residues, 1 model selected |
26571 | | |
26572 | | > color sel gammav3 |
26573 | | |
26574 | | > select #37/M |
26575 | | |
26576 | | 4552 atoms, 4624 bonds, 582 residues, 1 model selected |
26577 | | |
26578 | | > color sel gammav3 |
26579 | | |
26580 | | > select add #37 |
26581 | | |
26582 | | 27347 atoms, 27860 bonds, 1 pseudobond, 3435 residues, 2 models selected |
26583 | | |
26584 | | > select subtract #37 |
26585 | | |
26586 | | Nothing selected |
26587 | | |
26588 | | > hide #!37 models |
26589 | | |
26590 | | > show #!38 models |
26591 | | |
26592 | | > select #38/M |
26593 | | |
26594 | | 4552 atoms, 4624 bonds, 582 residues, 1 model selected |
26595 | | |
26596 | | > color sel gammav3 |
26597 | | |
26598 | | > select #39/m |
26599 | | |
26600 | | 4552 atoms, 4624 bonds, 582 residues, 1 model selected |
26601 | | |
26602 | | > color sel gammav3 |
26603 | | |
26604 | | > select add #39 |
26605 | | |
26606 | | 27347 atoms, 27860 bonds, 1 pseudobond, 3435 residues, 2 models selected |
26607 | | |
26608 | | > select subtract #39 |
26609 | | |
26610 | | Nothing selected |
26611 | | |
26612 | | > hide #!38 models |
26613 | | |
26614 | | > show #!39 models |
26615 | | |
26616 | | > hide #!39 models |
26617 | | |
26618 | | > show #!40 models |
26619 | | |
26620 | | > select #40/m |
26621 | | |
26622 | | 2567 atoms, 2609 bonds, 330 residues, 1 model selected |
26623 | | |
26624 | | > color sel gammav3 |
26625 | | |
26626 | | > select #40/n |
26627 | | |
26628 | | 930 atoms, 941 bonds, 118 residues, 1 model selected |
26629 | | |
26630 | | > color sel gammav3 |
26631 | | |
26632 | | > select #40/o |
26633 | | |
26634 | | 1064 atoms, 1080 bonds, 1 pseudobond, 135 residues, 2 models selected |
26635 | | |
26636 | | > color sel gammav3 |
26637 | | |
26638 | | > select #41/m |
26639 | | |
26640 | | 2567 atoms, 2609 bonds, 330 residues, 1 model selected |
26641 | | |
26642 | | > hide #!40 models |
26643 | | |
26644 | | > show #!41 models |
26645 | | |
26646 | | > color sel gammav3 |
26647 | | |
26648 | | > select #41/n |
26649 | | |
26650 | | 930 atoms, 941 bonds, 118 residues, 1 model selected |
26651 | | |
26652 | | > color sel gammav3 |
26653 | | |
26654 | | > select #41/o |
26655 | | |
26656 | | 1064 atoms, 1080 bonds, 1 pseudobond, 135 residues, 2 models selected |
26657 | | |
26658 | | > color sel gammav3 |
26659 | | |
26660 | | > select add #41 |
26661 | | |
26662 | | 27356 atoms, 27866 bonds, 2 pseudobonds, 3436 residues, 2 models selected |
26663 | | |
26664 | | > select subtract #41 |
26665 | | |
26666 | | Nothing selected |
26667 | | |
26668 | | > hide #!41 models |
26669 | | |
26670 | | > show #!42 models |
26671 | | |
26672 | | > select #42/m |
26673 | | |
26674 | | 4552 atoms, 4624 bonds, 582 residues, 1 model selected |
26675 | | |
26676 | | > color sel gammav3 |
26677 | | |
26678 | | > select add #42 |
26679 | | |
26680 | | 27347 atoms, 27860 bonds, 1 pseudobond, 3435 residues, 2 models selected |
26681 | | |
26682 | | > select subtract #42 |
26683 | | |
26684 | | Nothing selected |
26685 | | |
26686 | | > hide #!42 models |
26687 | | |
26688 | | > show #!43 models |
26689 | | |
26690 | | > select #43/m |
26691 | | |
26692 | | 2618 atoms, 2660 bonds, 337 residues, 1 model selected |
26693 | | |
26694 | | > color sel gammav3 |
26695 | | |
26696 | | > select #43/n |
26697 | | |
26698 | | 878 atoms, 889 bonds, 111 residues, 1 model selected |
26699 | | |
26700 | | > color sel gammav3 |
26701 | | |
26702 | | > select #43/o |
26703 | | |
26704 | | 1064 atoms, 1080 bonds, 1 pseudobond, 135 residues, 2 models selected |
26705 | | |
26706 | | > color sel gammav3 |
26707 | | |
26708 | | > select add #43 |
26709 | | |
26710 | | 27355 atoms, 27865 bonds, 2 pseudobonds, 3436 residues, 2 models selected |
26711 | | |
26712 | | > hide #!43 models |
26713 | | |
26714 | | > select subtract #43 |
26715 | | |
26716 | | Nothing selected |
26717 | | |
26718 | | > show #!44 models |
26719 | | |
26720 | | > select #44/o |
26721 | | |
26722 | | 1064 atoms, 1080 bonds, 1 pseudobond, 135 residues, 2 models selected |
26723 | | |
26724 | | > color sel gammav3 |
26725 | | |
26726 | | > select #44/n |
26727 | | |
26728 | | 878 atoms, 889 bonds, 111 residues, 1 model selected |
26729 | | |
26730 | | > color sel gammav3 |
26731 | | |
26732 | | > select #44/m |
26733 | | |
26734 | | 2618 atoms, 2660 bonds, 337 residues, 1 model selected |
26735 | | |
26736 | | > color sel gammav3 |
26737 | | |
26738 | | > select add #44 |
26739 | | |
26740 | | 27355 atoms, 27865 bonds, 2 pseudobonds, 3436 residues, 2 models selected |
26741 | | |
26742 | | > hide #!44 models |
| 1925 | [deleted to fit within ticket limits] |
| 1926 | |