1 | # Load model
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2 | open 7otq
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3 |
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4 | # Name orientations to make it easier to come back to them
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5 |
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6 | ## Orient in disc view and zoom in to fill camera view
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7 | turn x -90
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8 | turn z -5
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9 | view #1
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10 | view name disc
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11 |
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12 | ## Orient in dyad view
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13 | view disc
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14 | turn x 90
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15 | turn z -5
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16 | turn x -5
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17 | view name dyad
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18 |
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19 | ## Orient in gyres view
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20 | view dyad
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21 | turn x 180
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22 | turn z -5
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23 | view name gyres
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24 |
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25 | ## Orient to look at H4 tail
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26 | view gyres
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27 | turn y 95
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28 | turn x 45
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29 | turn y 30
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30 | view name H4tailInteraction
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31 | view H4tailInteraction
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32 | turn y -50
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33 | turn x -15
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34 | turn y -5
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35 | view name H4tailInteraction2
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36 |
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37 | # Find correct protein secondary structure assignments in atomic model
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38 | dssp protein
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39 |
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40 | # Load other PDB entries to compare to
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41 | ## Yeast Snf2 in apo, ADP-BeFx and ADP bound states
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42 | open 5z3l
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43 | open 5z3u
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44 | open 5z3o
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45 | ## Yeast Isw1 in ADP-BeFx and ADP bound states
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46 | open 6jyl
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47 | open 6iro
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48 | ## Human CHD4 in ADP-BeFx bound state
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49 | open 6ryr
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50 |
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51 | # Align them all to our model, based on H3 chains
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52 | mmaker #2-7/a,e to #1/a,e
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53 |
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54 | # Name chains to make the rest easier to read
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55 | name H3 /a/e
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56 | name H4 /b/f
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57 | name H2A /c/g
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58 | name H2B /d/h
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59 | name histones /a-h
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60 | name DNA /i/j
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61 | name mainNuc #1/a-j
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62 | name otherNucs #2-7/a-j
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63 | name ALC1 #1/k
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64 | name NATPase #1/k:42-266
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65 | name CATPase #1/k:267-500
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66 | name H4tail #1/b:16-24
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67 | name Snf2Apo #2/o
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68 | name Snf2ADPBeF #3/o
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69 | name Snf2ADP #4/o
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70 | name Snf2 #2-4/o
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71 | name Snf2NATPase #2-4/o:670-1000
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72 | name Isw1ADPBeF #5/k
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73 | name Isw1ADP #6/l
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74 | name Isw1 #5/k#6/l
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75 | name Isw1NATPase #5/k:101-417#6/l:101-417
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76 | name CHD4 #7/w:727-1212
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77 | name CHD4NATPase #7/w:446-960
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78 | name bindingFactors #1/k#2-4/o #5/k#6/l #7/w:727-1212
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79 |
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80 | # Apply color code
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81 | color H3 blue
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82 | color H4 green
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83 | color H2A yellow
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84 | color H2B red
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85 | color DNA silver
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86 | color ALC1 deeppink
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87 | color NATPase salmon
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88 | color Snf2 teal
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89 | color Snf2NATPase turquoise
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90 | color Isw1 dodgerblue
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91 | color Isw1NATPase skyblue
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92 | color CHD4 limegreen
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93 | color CHD4NATPase greenyellow
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94 |
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95 | # Global display options
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96 | set bgColor white
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97 | lighting soft
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98 | graphics silhouettes true
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99 |
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100 | # Adjust display of atomic models
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101 | hide atoms
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102 | hide cartoons
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103 | show mainNuc cartoons
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104 | show bindingFactors cartoons
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105 | hide #!2-7 models
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106 | transparency mainNuc 75 target c
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107 | cartoon style protein modeHelix tube radius 2 sides 24
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108 |
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109 | view gyres
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110 |
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111 | # Make figures
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112 | ## Superimposition of Snf2Apo
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113 | show #!2 models
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114 | save ALC1-Snf2Apo_superimposition_gyres-view.png transparentbackground true supersample 3
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115 | hide #!2 models
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116 | ## Superimposition of Snf2ADPBeF
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117 | show #!3 models
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118 | save ALC1-Snf2ADPBeF_superimposition_gyres-view.png transparentbackground true supersample 3
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119 | hide #!3 models
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120 | ## Superimposition of Snf2ADP
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121 | show #!4 models
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122 | save ALC1-Snf2ADP_superimposition_gyres-view.png transparentbackground true supersample 3
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123 | hide #!4 models
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124 | ## Superimposition of IswADPBeF
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125 | show #!5 models
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126 | save ALC1-Isw1ADPBeF_superimposition_gyres-view.png transparentbackground true supersample 3
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127 | hide #!5 models
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128 | ## Superimposition of IswADP
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129 | show #!6 models
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130 | save ALC1-Isw1ADP_superimposition_gyres-view.png transparentbackground true supersample 3
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131 | hide #!6 models
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132 | ## Superimposition of CHD4ADPBeF
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133 | show #!7 models
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134 | save ALC1-CHD4ADPBeF_superimposition_gyres-view.png transparentbackground true supersample 3
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135 | #hide #!7 models
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