Ticket #3482: 6NQ3_Suz12_AS_event.cxc

File 6NQ3_Suz12_AS_event.cxc, 3.4 KB (added by arecco.niccolo@…, 5 years ago)

Added by email2trac

Line 
1# Fetch ID
2open 6NQ3
3
4# Set up the structures to view
5select all ; show sel cartoons; style sel stick; hide sel atoms
6select /A,B,C,G,D,H ; hide sel cartoons
7select /E ; color (#!1 & sel) purple
8select /F ; color (#!1 & sel) cyan
9select clear
10
11# Set starting view
12set bgColor white
13lighting soft
14graphics silhouettes true
15view
16view name start
17
18# Start the movie production
19movie record supersample 1 size 720,720 limit 5000 directory ~/Desktop/tmp_chimera pattern Frame_n*
20
21# Set starting through the hole of Rbbp4
22turn z -70
23turn x 24
24turn y -40
25view name hole
26wait 5
27
28# colour Histone-binding protein RBBP4 or subunit C of CAF1 complex; Region: CAF1C_H4-bd; pfam12265
29select sequence EEYKIWKKNTPFLYDLVMTHALEWPSLTAQWLPDVTRPEGKDFSIHRLVLGTHTSDEQNHLVIASVQLP ; color sel orange
30select clear ; wait 10
31
32# Colour the WD40 repeat 1
33select sequence NHEGEVNRARYMPQNPCIIATKTPSSDVLVFDYT ; color sel spring green
34select clear ; wait 10
35# Colour the WD40 repeat 2
36select sequence GHQKEGYGLSWNPNLSGHLLSASDDHTICLWD ; color sel spring green
37select clear ; wait 10
38# WD40 repeat 3
39select sequence GHTAVVEDVSWHLLHESLFGSVADDQKLMIWD ; color sel spring green
40select clear ; wait 10
41# WD40 repeat 4
42select sequence AHTAEVNCLSFNPYSEFILATGSADKTVALWD ; color sel spring green
43select clear ; wait 10
44# WD40 repeat 5
45select sequence SHKDEIFQVQWSPHNETILASSGTDRRLNVWD ; color sel spring green
46select clear ; wait 10
47# WD40 repeat 6
48select sequence GHTAKISDFSWNPNEPWVICSVSEDNIMQVWQ ; color sel spring green
49select clear ; wait 10
50
51# Show altenative exon
52select sequence RKTFKVDDMLSKVEKMKGEQESH
53color sel red
54select clear
55wait 10
56
57
58# All structure overview
59turn x 20 120 rock 120 ; wait
60turn y 35 150 rock 150 ; wait
61turn y 4 90 ; wait
62
63
64# Show first point of interaction with yellow region
65turn y 1 50 ; wait
66turn x 1 35 ; wait
67zoom 1.5 80 ; wait
68move x 0.75 15 ; move y -0.75 15 ; wait
69view name side
70
71# Show side chains interacting
72select /E:9-28 /E:32-39
73show sel atoms
74hbonds sel
75select clear
76wait 20
77turn x 15 60 rock 60 ; wait
78turn y 15 60 rock 60; wait
79
80# remove hydrogen bonds and
81~hbonds
82select /E:9-28 /E:32-39
83hide sel atoms
84select clear
85wait 5
86
87# go back
88move y 0.75 15 ; move x -0.75 15 ; wait
89zoom 0.5 80 ; wait
90turn -x 1 35 ; wait
91turn -y 1 50 ; wait
92view hole
93# the going back to 'hole' position is a bit abrupt
94
95# Close up to region 1
96move x 0.75 25 ; move y -0.5 25 ; wait 25
97turn x 0.5 25 ; zoom 3 80 ; wait 80
98turn x 0.75 25 ; move y 0.5 25 ; wait 25
99view name zoom1
100
101# Show residues interacting with R129
102select /F:129 /E:409 /E:346 ; show sel atoms; color (#!1 & sel) byelement ; hbonds sel
103select clear
104wait 15
105turn -y 15 120 rock 120 ; wait
106turn x 15 60 rock 130 ; wait
107wait 15
108view name zoom2
109
110# Move to next site Asp 129
111turn y -1.5 25 ; move x -0.5 25 ; wait 25
112turn x -1 25 ; turn z 1 25 ; move x 0.4 25 ; wait 25
113
114# Show residues interacting with ASP135
115select /F:135 /E:285,304,330 ; show sel atoms; color (#!1 & sel) byelement ; hbonds sel
116select clear
117turn y -10 120 rock 180 ; wait
118turn x 15 120 rock 120 ; wait
119wait 20
120view name zoom3
121
122# zoom out and move to other region
123zoom 0.5 50 ; turn -y 1 150 ; wait
124move x 0.75 15 ; wait
125turn x 1 20 ; wait
126zoom 1.5 50
127
128# Select last interaction area
129select /F:370,375 /E:270,309,314
130show sel atoms
131hbonds sel
132select clear
133
134# Final show
135turn x 15 60 rock 130
136turn -y 15 60 rock 130
137
138# Print status in log
139movie status
140
141# Export movie
142movie encode ~/Desktop/Suz12_test1.mp4 format h264 quality low wait true verbose false
143# close