[Chimera-users] Stuck in adding residue in the C-terminal of a segment

[Prathit Chatterjee]

Dear Experts,

This is regarding adding an ALA residue in the C-terminal of a specific
protein domain, with CHIMERA.

I have a protein-complex with multiple domains and chains.
I want to add an ALA residue (101-th position) in the chain D, after the
100th residue which is PRO.

I followed the tutorial and have given the following command in command
line:

*addaa ala,101 :100.d*

The following outcome was: Couldn’t find ‘O’ backbone atom of PRO 100.D.

Request you to kindly let me know what the possible solution could be, so
that I add an ALA residue in the C-terminal of Chain D.

Thank you in advance,
Regards,
Prathit Chatterjee


On Mon, Jun 28, 2021 at 4:48 PM Prathit Chatterjee <
prathit.biophysics at gmail.com> wrote:

> Dear Experts,
>
> This is regarding adding an ALA residue in the C-terminal of a specific
> protein domain, with CHIMERA.
>
> I have a protein-complex with multiple domains and chains.
> I want to add an ALA residue (101-th position) in the chain D, after the
> 100th residue which is PRO.
>
> I followed the tutorial and have given the following command in command
> line:
>
> *addaa ala,101 :100.d*
>
> The following outcome was: Couldn’t find ‘O’ backbone atom of PRO 100.D.
>
> Request you to kindly let me know what the possible solution could be, so
> that I add an ALA residue in the C-terminal of Chain D.
>
> Thank you in advance,
> Regards,
> Prathit Chatterjee
>
>
>
>
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