[Chimera-users] Fc dimer fall apart after fusion with a GS linker

Elaine Meng meng at cgl.ucsf.edu
Mon Aug 16 07:58:35 PDT 2021

Hi TW,
My only guess is that these parts are originally in two different models and you are using the Join Models panel in Build Structure to form "C-N peptide bond."  In that case, make sure you are setting the "Move atoms on [...] side" option so that it moves the linker part, not the Fc part.  You said the linker is the part with "N" selected, so try:

Move atoms on [selected N atom] side

See the help:

I hope this helps,
Elaine C. Meng, Ph.D.                       
UCSF Chimera(X) team
Department of Pharmaceutical Chemistry
University of California, San Francisco

> On Aug 15, 2021, at 4:47 PM, tiger wang via Chimera-users <chimera-users at cgl.ucsf.edu> wrote:
> Dear Ms/Mr helper:
> I encounted an issue when I try to fuse one (G4S)x linker to one of the Fc "C" terminal using "join bond". Once selected the "C" atom on Fc C terminal and "N" atom on (G4S)x N terminal and hit "apply" button, the Fc dimer fall apart but the (G4S)x linker was connected to the C terminal of Fc. How to avoid the Fc dimer from falling apart? 
> Thanks very much for your help
> Sincerely 
> TW

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