[Chimera-users] building modeller loops refinement

[ARMIN HODAEI]

Dear Elaine,


   Thank you for your email. So I build missing residues for my both chains
separately, is there a way to combine these two chains after that process??
I mean, I have now two chains which they are both the outputs of Modeller,
how to create a single PDB out of two processed PDB.

Cheers,

On Tue, Apr 4, 2017 at 6:42 PM, Elaine Meng <meng at cgl.ucsf.edu> wrote:

> Dear Armin,
> Sorry, the Chimera-Modeller interface does not allow modeling more than
> one chain.  Modeling a multimer or complex of multiple chains requires
> running Modeller separately.
>
> <http://www.rbvi.ucsf.edu/chimera/docs/ContributedSoftware/
> multalignviewer/modeller.html>
>
> Regards,
> Elaine
> ----------
> Elaine C. Meng, Ph.D.
> UCSF Computer Graphics Lab (Chimera team) and Babbitt Lab
> Department of Pharmaceutical Chemistry
> University of California, San Francisco
>
> > On Apr 4, 2017, at 4:56 AM, ARMIN HODAEI <ahodaei15 at ku.edu.tr> wrote:
> >
> > Dear Chimera Users,
> >     I want to use refining loop option to build missing residues for my
> target protein. First I get the FASTA file structure of protein then I load
> it into Chimera, after that in the Structure tab I select load structure,
> then I see the protein. After that Again in the "Structure" tab I select
> Modeller(loops/refinement). The rest is easy for creating the new structure
> PDB. But the problem is that I want whole protein to be treated in this
> refinement, but it only chose one chain, How can I do this for both chains
> at once?
> > Sincrely,
> > --
> > Armin Hodaei
> > Department of Physics,
> > Faculty of Arts and Sciences
> > Koc University, Istanbul
>
>


-- 
Armin Hodaei
Department of Physics,
Faculty of Arts and Sciences
Koc University, Istanbul
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