[Chimera-users] maintain .seg alignment to .mrc between sessions

Amy Jobe abj2111 at columbia.edu
Wed Mar 23 10:30:43 PDT 2016

When I segment an 80S ribosome into individual ribosomal proteins and
rRNAs, it takes some time and I often need to close and reopen my session.
Whenever I reopen my session, though, my .seg file is no longer aligned to
my .mrc map, and I have to spend time manually (and probably slightly
inaccurately) re-aligning them.  I've tried using the Associate Selected
option in the daily build, although I'm not sure if it's meant to maintain
alignment, and it hasn't fixed the problem.  Can anyone help me with this?
I'm using the daily build and the most recent stable version on Mac.

Amy Jobe

PhD candidate, Joachim Frank group
Department of Biological Sciences
Columbia University
2-221 P & S Black Building
650 W. 168th St.
New York, NY 10032
Telephone:  (315) 879-8192
E-mail:  abj2111 at columbia.edu
Lab website:  http://franklab.cpmc.columbia.edu/franklab/
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