[Chimera-users] need help to plot electrostatic surface charge distribution

[Elaine Meng]

Dear Ashok,
If the NMR structure has multiple conformations, you first have to decide which one you want and close the others.  About that, see this post from yesterday:
<http://plato.cgl.ucsf.edu/pipermail/chimera-users/2016-April/012204.html>

Next, delete hydrogens, for example, command:  delete H
You could also do it with the menu.  To start learning Chimera, you could try the “getting started” tutorials:
<http://www.rbvi.ucsf.edu/chimera/docs/UsersGuide/frametut.html>

Next, show electrostatic potential coloring on the surface.  There is already a step-by-step tutorial for that, and it includes two different ways (Coulombic and Poisson-Boltzmann):
<http://www.rbvi.ucsf.edu/chimera/docs/UsersGuide/tutorials/surfprop.html>

Best,
Elaine
----------
Elaine C. Meng, Ph.D. 
UCSF Computer Graphics Lab (Chimera team) and Babbitt Lab
Department of Pharmaceutical Chemistry
University of California, San Francisco

> On Apr 26, 2016, at 5:13 AM, ashok rout <ashok9869 at gmail.com> wrote:
> 
> Hi,
>  I have NMR structure of a protein. I want to plot electrostatic surface charge distribution. Can you please write me step by step commands how I can do it.
> I want to remove hydrogens first and then want to plot electrostatic surface charge distribution.
> Ashok
> 
> -- 
> thanks & regards,
> Ashok