[Chimera-users] saving multiple maps
Thomas Goddard
goddard at cgl.ucsf.edu
Mon May 19 14:30:18 PDT 2008
Hi Jason,
Saving multiple maps in a single file is possible with a file format
that is only used by Chimera. I don't recommend it. Maybe you are
interested in adding one map to another. Chimera doesn't do that
currently though I could add it.
Chimera does allow you to recalculate a map at the grid points of a
second map using interpolation, called "resampling". The resulting map
can be saved and can be added point-wise to other maps having the same
grid using other software (e.g. EMAN). To resample a volume use the
"vop" command (volume operation) with the resample option, for example,
volume #1 resample onGrid #0
calculates the map with model id 1 on the grid of map with id 0 and
creates a new map.
http://www.cgl.ucsf.edu/chimera/docs/UsersGuide/midas/volume.html
If your goal is just to save the fitting of one map into a second map
then you can save the Chimera session. But if you want to be able to
open those maps in another program and have them align you would need to
do the above resampling so that both maps have the same grid and save
the resulting map. Then other programs will show both maps with the
desired alignment.
Here are more details about the Chimera format the can handle
multiple maps in one file. First, there are no commonly used file
formats that I know of for x-ray or EM maps that hold multiple maps.
Some formats of this sort are used in light microscopy for handling
multi-wavelength images. There is a prototype file format called
"Chimera map" based on HDF5 that can do keep many maps in one file, but
only Chimera reads and writes this format. You write multiple maps to
one cmap file with the "volume" command with "saveFormat cmap". The
"append True" option allows you to append to an existing file. I don't
recommend using this as it was written just to explore desirable
capabilities for a new EM file format.
http://www.cgl.ucsf.edu/chimera/docs/UsersGuide/midas/volume.html#output
Tom
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