| 122 | | --------------------------------------------------------------------------- |
| 123 | | 2010/2011 report |
| 124 | | --------------------------------------------------------------------------- |
| 125 | | |
| 126 | | June 2010 - March 2011 |
| 127 | | Created 23 [http://www.cgl.ucsf.edu/chimera/videodoc/videodoc.html video tutorials] |
| 128 | | demonstrating different analysis techniques of electron microscopy density maps and |
| 129 | | molecular models using UCSF Chimera. Most videos are 5 to 10 minute long computer |
| 130 | | screen captures with audio narration showing step-by-step analysis. Each was made to |
| 131 | | address specific questions posed by users. Streaming videos are distributed via the |
| 132 | | RBVI web site using an in-browser Flash movie player. Total preparation time 50 hours. |
| 133 | | - Tom Goddard |
| 134 | | |
| 135 | | June 9, 2010 and July 7, 2010 and July 21, 2010 |
| 136 | | Demonstration showing DNA for total of 38 students ages 8-12 from |
| 137 | | [http://www.celsiusandbeyond.com/ Celsius and Beyond] |
| 138 | | summer science camp on 3 separate days. |
| 139 | | Organized with Auritte Cohen-Ross. Showed light |
| 140 | | microscopy volume time series of chromosomes in dividing cells, electron |
| 141 | | microscopy of chromosomes, DNA molecular structure, nucleosomes and chromatin |
| 142 | | (http://www.cgl.ucsf.edu/chimera/dna-jun2010/dnademo.html). |
| 143 | | 8 hours preparation. 1 hour demonstration in RBVI visualization room. |
| 144 | | Many questions from kids "What happens if you eat a bubble?". |
| 145 | | - Tom Goddard |
| 146 | | |
| 147 | | June 16-17, 2010 |
| 148 | | Summer School '10 : Python Applied to Computational Chemistry and Molecular Modelling, |
| 149 | | Universitat Autònoma de Barcelona, Spain. ~50 attendees. |
| 150 | | http://hpc.xrqtc.com/index.php?option=com_content&view=article&id=118:sm10-module-c&catid=54:recent-conference&Itemid=75 |
| 151 | | - Conrad Huang |
| 152 | | |
| 153 | | July 11, 2010 |
| 154 | | Presentation on Cytoscape at ISMB 2010 to an audience of approximately 60. |
| 155 | | http://www.iscb.org/cms_addon/conferences/ismb2010/technologytrack.php |
| 156 | | Boston, MA. |
| 157 | | The presentation provided an introduction to Cytoscape's capabilities and |
| 158 | | some of the new features in Cytoscape 2.7 along with some new and recently |
| 159 | | enhanced plugins. |
| 160 | | - Scooter Morris |
| 161 | | |
| 162 | | July 13, 2010 |
| 163 | | "Network visualization and analysis using Cytoscape" Birds of a Feather |
| 164 | | session at ISMB 2010. Scooter Morris facilitated this session to solicit |
| 165 | | input from Cytoscape users and developers. About 30 participants. |
| 166 | | |
| 167 | | July 18, 2010 |
| 168 | | "Cytoscape User Tutorial". 2 hour introductory user tutorial given to about |
| 169 | | 50 users as part of the annual Cytoscape Retreat. |
| 170 | | - Scooter Morris |
| 171 | | |
| 172 | | July 18, 2010 |
| 173 | | Plugin demonstration on CyCommandHandlers as part of the Cytoscape Retreat to |
| 174 | | about 100 participants. University of Michigan, Ann Arbor, MI. |
| 175 | | - Scooter Morris |
| 176 | | |
| 177 | | July 22, 2010 |
| 178 | | Gave two 20 minute demonstrations to 24 students, part of [http://www.ucop.edu/cosmos/ COSMOS] science |
| 179 | | program. Organized by Ken Dill. Showed DNA atomic models, small duplex, nucleosomes, |
| 180 | | chromatin filament model, chromosomes seen in electron microscopy. Used RBVI visualization room. |
| 181 | | 8 hours preparation. |
| 182 | | - Tom Goddard |
| 183 | | |
| 184 | | August 16, 2010 |
| 185 | | Tutorial given at CSB 2010, Stanford: "Analysis and Visualization of Biological Networks". |
| 186 | | This was a 1/2 day tutorial given to 15 participants that covered visualization |
| 187 | | and analytical approaches to understanding biological networks. Second half of |
| 188 | | tutorial introduced Cytoscape as a tool and provided a hands-on exercise. |
| 189 | | - Scooter Morris |
| 190 | | |
| 191 | | August 16, 2010 |
| 192 | | 10 minute SFLD demo, followed by 2 hour SFLD/cytoscape tutorial. Attendees worked through |
| 193 | | exercises designed to give them basic familiarity with manipulating a cytoscape sequence |
| 194 | | and/or structure similarity network of their superfamily of choice, of the sort downloaded |
| 195 | | from the SFLD, to investigate questions of biological interest. |
| 196 | | Audience: ~25 grad students, postdocs, staff from the EFI. 15 hours preparation. |
| 197 | | - Shoshana Brown |
| 198 | | |
| 199 | | September 29, 2010 |
| 200 | | 40-minute presentation to ~14 students in UCSF BP204, "Macromolecular Interactions |
| 201 | | and Assemblies" class. Included 5 min on history of Computer Graphics Lab, the remainder |
| 202 | | a Chimera demonstration: basic usage, morphing, viewing sequences, showing attribute |
| 203 | | values, building multimers, stereo. Prep time 12 hours including updating the previous |
| 204 | | year's presentation, testing with the current version of Chimera, rehearsing. |
| 205 | | - Elaine Meng, Eric Pettersen |
| 206 | | |
| 207 | | September 29, 2010 |
| 208 | | 30-minute presentation to ~50 attendees, then followed by 90-minute tutorial to ~20 students at |
| 209 | | Department of Computational and System Biology, School of Medicine and Clinical Translational |
| 210 | | Science Institute, University of Pittsburgh. The presentation part, titled "Introduction to UCSF |
| 211 | | Chimera, an Extensible Molecular Modeling System", covered the brief history of molecular |
| 212 | | visualization, RBVI history and Chimera highlighted features. The tutorial part went over |
| 213 | | several basic examples in the "getting started" document. Prep time 40 hours. |
| 214 | | - YZ (Zheng Yang) |
| 215 | | |
| 216 | | October 12-13, 2010 |
| 217 | | Poster presentation on segmentation and measurement of bacteria in termite gut at NIH workshop |
| 218 | | "Recent Advances and Future Prospects for Visualizing Macromolecular Complexes and |
| 219 | | Cellular Structures". 170 participants. 12 hours preparation. |
| 220 | | Discussions of research projects with about 10 attendees |
| 221 | | https://conferences.niaid.nih.gov/vbbethesda/ |
| 222 | | - Tom Goddard |
| 223 | | |
| 224 | | October 25, 2010 |
| 225 | | Invited talk, "Annotation Error in Public Databases," at the 2010 Pathway Tools Workshop, |
| 226 | | Menlo Park, CA. http://bioinformatics.ai.sri.com/ptools10/agenda.html |
| 227 | | Discussed SFLD and using sequence similarity networks in Cytoscape. ~100 attendees. |
| 228 | | - Alexandra Schnoes |
| 229 | | |
| 230 | | October 27, 2010 |
| 231 | | 45-minute Chimera teaching demonstration to ~30 students for a graduate course in protein |
| 232 | | engineering at UC Berkeley (taught by Danielle Tullman-Ercek, Asst. Professor, Dept. of |
| 233 | | Chemical and Biomolecular Engineering, UC Berkeley). Covered basic usage, finding H-bonds, |
| 234 | | morphing, saving trajectories as movies, viewing sequences, showing attribute values, |
| 235 | | evaluating rotamers, performing virtual mutations, building multimers, and using stereo. |
| 236 | | Prep time 20 hours including adding more material to the previously developed demo, |
| 237 | | testing with the current version of Chimera, and rehearsing. |
| 238 | | - Elaine Meng, Eric Pettersen |
| 239 | | |
| 240 | | November 18, 2010 |
| 241 | | Two 45-minute demonstrations showing termite gut and OpcA meningitis bacterium protein to |
| 242 | | SMART team Lincoln high school students. This is a UCSF Science and Health Education |
| 243 | | Partnership (SEP) program. Showed FIBSEM data, spirochetes, flagella, handedness, |
| 244 | | endo-flagella, protein morphing, hydrophobic and electrostatic surface coloring to identify |
| 245 | | transmembrane and binding regions. 16 students. 6 hours preparation. |
| 246 | | - Tom Goddard |
| 247 | | |
| 248 | | December 2010 |
| 249 | | Chimera used to create image for RBVI holiday card: glutamine synthetase 12-mer styled as an |
| 250 | | "architectural plan" (black outlines around transparent ribbons on aged parchment background). Estimated 36 person-hours of work (including preparing candidate images, voting, format converting). |
| 251 | | - YZ (Zheng Yang) |
| 252 | | |
| 253 | | December 2, 2010 |
| 254 | | Two 45-minute demonstrations showing termite gut and OpcA meningitis bacterium protein to |
| 255 | | SMART team Galileo high school students. This is a UCSF Science and Health Education Partnership (SEP) |
| 256 | | program. Showed FIBSEM data, spirochetes, flagella, handedness, endo-flagella, protein morphing, |
| 257 | | hydrophobic and electrostatic surface coloring to identify transmembrane and binding regions. |
| 258 | | 20 students. 4 hours preparation. |
| 259 | | - Tom Goddard |
| 260 | | |
| 261 | | December 8, 2010 |
| 262 | | Two hour discussion and Chimera demonstration of myosin thick filament regulation for |
| 263 | | UCSF Tetrad graduate program macromolecules discussion group. Looked at thick filament architecture and |
| 264 | | myosin 2 heavy and light chain atomic models using stereo 3d visualization. 5 graduate |
| 265 | | students, led by professor Zev Gartner. 8 hours preparation. |
| 266 | | - Tom Goddard |
| 267 | | |
| 268 | | December 13, 2010 |
| 269 | | Chimera Release Party and RBVI Open House (2-5 pm in Genentech Hall N453, |
| 270 | | UCSF Mission Bay) for local researchers and students to meet the Chimera |
| 271 | | developers, see demonstrations and discuss features, tour the RBVI |
| 272 | | facilities, and share refreshments. Estimated 50 attendees, 3 hours for the event, |
| 273 | | 24 person-hours preparation, publicity, and cleanup. |
| 274 | | - Chimera team |
| 275 | | |
| 276 | | January 12, 2010 |
| 277 | | Created 3 videos showing how to explore virus models for HHMI Viral Outbreak |
| 278 | | CDROM distribution to US high schools. Videos totaled 15 minutes and explained |
| 279 | | Chimera virus display, finding viruses structures on the web, and antibodies |
| 280 | | bound to West Nile virus. Expected distribution is several thousand CDs. |
| 281 | | Preparation time 15 hours. |
| 282 | | - Tom Goddard |
| 283 | | |
| 284 | | January 13, 2011 |
| 285 | | 20 minute SFLD demo, followed by 10 minute Q & A, showing how the SFLD can be used to get |
| 286 | | information about the biological context of an uncharacterized target of interest. |
| 287 | | Audience: ~20 EFI investigators and other staff. 15 hours preparation. |
| 288 | | - Shoshana Brown |
| 289 | | |
| 290 | | February 11, 2011 |
| 291 | | Demonstration showing immune system structures to 7 visiting candidate UCSF graduate |
| 292 | | students. West Nile virus with bound antibodies, cytotoxic T-cell EM data. Also |
| 293 | | showed 3d printer and plastic molecular models. Two 35 minute demonstrations. |
| 294 | | 4 hours preparation. |
| 295 | | - Tom Goddard |
| 296 | | |
| 297 | | February 11, 2011 |
| 298 | | Seminar at University of Toronto covering base cytoscape and RBVI plugins to group |
| 299 | | of about 50. The seminar was 2 hours and included introductory topics as well as |
| 300 | | some advanced discussion. |
| 301 | | 10 hours preparation |
| 302 | | - Scooter Morris |
| 303 | | |
| 304 | | February 18, 2011 |
| 305 | | Demonstration showing clathrin cages to 4 visiting candidate UCSF graduate |
| 306 | | students. Clathrin cage triskelions, molecular assembly model, animation. Also |
| 307 | | showed 3d printer and plastic molecular models. Two 35 minute demonstrations. |
| 308 | | 4 hours preparation. |
| 309 | | - Tom Goddard |
| 310 | | |
| 311 | | March 14-17, 2011 |
| 312 | | Chimera tutorial at [http://blake.bcm.edu/emanwiki/Ws2011/Agenda EMAN single particle reconstruction workshop], |
| 313 | | showing fitting atomic models in electron microscopy maps, symmetry, presentation images |
| 314 | | using Rous sarcoma virus data. Produced |
| 315 | | [http://www.cgl.ucsf.edu/chimera/videodoc/RousSarcomaVirus/ video version] of the workshop tutorial. |
| 316 | | Tutorial was 1 hour with ~100 participants at University of Houston. |
| 317 | | Answered approximately 30 Chimera usage questions during 4-day workshop |
| 318 | | and demonstrated features to individual workshop participants, about 10 hours total time. |
| 319 | | 20 hours preparation. |
| 320 | | - Tom Goddard |
| 321 | | |
| 322 | | March 19, 2011 |
| 323 | | Half day Cytoscape tutorial at [http://vizbi.org/2011/ VizBI 2011] to 20 participants, showing basic Cytoscape plus clusterMaker, structureViz, chemViz, and nodeCharts. This was followed by a half day workshop where participants brought their own data sets and collaborated on analyses and visualizations. |
| 324 | | |
| 325 | | 16 hours preparation |
| 326 | | - Scooter Morris |