| 7 | | --------------------------------------------------------------------------- |
| 8 | | 2008/2009 report |
| 9 | | --------------------------------------------------------------------------- |
| 10 | | Jun 3, 2008 |
| 11 | | UCSF Library Course: |
| 12 | | Molecular Visualization with UCSF Chimera: Getting Started, |
| 13 | | 9-11am at the UCSF Mission Bay Campus. |
| 14 | | |
| 15 | | This session will go over the basics of molecular visualization and |
| 16 | | provide an introduction to how to use UCSF Chimera to load, visualize, |
| 17 | | and manipulate protein structures. Topics include: installing Chimera; |
| 18 | | Chimera menus and commands; fetching protein structures from ModBase |
| 19 | | and PDB; selections and actions; depictions; key tools: Side View, Model |
| 20 | | Panel, and the Selection Panel; and saving your work: sessions and images. |
| 21 | | |
| 22 | | Participants should have a basic understanding of protein structure. |
| 23 | | All participants will receive a CD with UCSF Chimera installers for Mac, |
| 24 | | PC, and Linux as well as example files and additional exercises. |
| 25 | | - Scooter Morris, Conrad Huang |
| 26 | | |
| 27 | | Jun 17, 2008 |
| 28 | | UCSF Library Course: |
| 29 | | Molecular Visualization with UCSF Chimera: Basic Tools, |
| 30 | | 9-11am at the UCSF Mission Bay Campus. |
| 31 | | |
| 32 | | This session will cover general structure visualization and analysis |
| 33 | | with Chimera. Topics include: identifying hydrogen bonds and contacts; |
| 34 | | measuring distances and angles, rotating bonds; viewing amino acid |
| 35 | | sidechain rotamers, "mutating" residues; displaying attributes such as |
| 36 | | B-factor and residue hydrophobicity; superimposing and comparing |
| 37 | | structures, morphing; viewing NMR ensembles and molecular dynamics |
| 38 | | trajectories. |
| 39 | | |
| 40 | | Participants should have an acquaintance with molecular graphics and |
| 41 | | protein structure, although expertise with Chimera is not required. |
| 42 | | Participants are encouraged to bring structures of interest (or note |
| 43 | | their Protein Data Bank IDs for retrieval in the lab) as "real-life" |
| 44 | | examples to supplement the exercises that will be provided. |
| 45 | | - Elaine Meng, Scooter Morris |
| 46 | | |
| 47 | | Jul 1, 2008 |
| 48 | | UCSF Library Course: |
| 49 | | Exploring Sequence-Structure Relationships with UCSF Chimera, |
| 50 | | 9-11am at the UCSF Mission Bay Campus. |
| 51 | | |
| 52 | | This session will cover working with protein sequence alignments together |
| 53 | | with 3D structures. Topics include: Web resources for protein family |
| 54 | | sequence alignments; sequence-structure association and crosstalk; |
| 55 | | calculating alignment conservation and displaying it on structures; |
| 56 | | using the alignment to match (superimpose) structures; matching |
| 57 | | structures without a pre-existing alignment; creating sequence alignments |
| 58 | | from multiple structure superpositions; customizing alignment appearance |
| 59 | | in Chimera; editing and saving. |
| 60 | | |
| 61 | | Participants should have at least a little experience with Chimera and |
| 62 | | a basic understanding of protein structure and sequence alignments. |
| 63 | | Participants are encouraged to bring data on systems of interest |
| 64 | | (structure IDs, and if available, corresponding sequence alignments) |
| 65 | | as "real-life" examples to supplement the exercises that will be provided. |
| 66 | | - Elaine Meng, Eric Pettersen |
| 67 | | |
| 68 | | Jul 15, 2008 |
| 69 | | UCSF Library Course: |
| 70 | | Working with EM and X-ray Density Maps in UCSF Chimera, |
| 71 | | 9-11am at the UCSF Mission Bay Campus. |
| 72 | | |
| 73 | | This session offers hands-on training for studying density maps, |
| 74 | | especially those from electron microscopy. You'll look at maps of phage |
| 75 | | K1E, a virus that infects E. coli, and learn to segment the protein coat |
| 76 | | from the packaged DNA genome, color to distinguish virus surface structures, |
| 77 | | morph to compare related viruses, and fit atomic models into the maps. |
| 78 | | The same techniques are applicable for crystallographic and tomographic |
| 79 | | density maps. Participants should have familiarity with Chimera. |
| 80 | | - Tom Goddard |
| 81 | | |
| 82 | | Jul 19, 2008 |
| 83 | | "Introduction to Molecular Visualization" tutorial at ISMB 2008 |
| 84 | | (16th Annual International Conference, Intelligent Systems for |
| 85 | | Molecular Biology, Toronto) |
| 86 | | http://www.iscb.org/ismb2008/tutorials.php |
| 87 | | Abstract: |
| 88 | | Projects such as Structural Genomics are providing increasing numbers |
| 89 | | of experimental protein and protein-complex structures. Furthermore, |
| 90 | | increasing numbers of theoretical models are being predicted from primary |
| 91 | | sequence. Biologists have an increasing need to understand and communicate |
| 92 | | the structures, functions and relationships between these protein and |
| 93 | | protein-complex structures. As a result, molecular visualization is |
| 94 | | becoming an important tool for the presentation and communication of |
| 95 | | the results of biological experiments and research. This tutorial will |
| 96 | | provide a basic foundation for the understanding of molecular structures |
| 97 | | through use of visualization tools. |
| 98 | | Attendees will learn the basics of molecular visualization and will be |
| 99 | | provided an overview of available tools and techniques for visualization, |
| 100 | | analysis and modeling of protein structure. To make these concepts more |
| 101 | | concrete, attendees will be shown the academic program UCSF Chimera in |
| 102 | | more detail, and receive instruction in its features and use. The field |
| 103 | | of structural biology is still changing, and new techniques are continually |
| 104 | | being developed. Attendees will be shown how they can add new analysis |
| 105 | | techniques and their own. |
| 106 | | - Scooter Morris, Eric Pettersen |
| 107 | | |
| 108 | | Jul 22, 2008 |
| 109 | | "StructureViz: Cytoscape and Chimera" technology track demo at ISMB 2008 |
| 110 | | (16th Annual International Conference, Intelligent Systems for |
| 111 | | Molecular Biology, Toronto) |
| 112 | | - Scooter Morris |
| 113 | | |
| 114 | | Jul 29, 2008 |
| 115 | | UCSF Library Course: |
| 116 | | Publication-Quality Images and Movies with UCSF Chimera, |
| 117 | | 9-11am at the UCSF Mission Bay Campus. |
| 118 | | |
| 119 | | This session provides hands-on training for creating images and movies of |
| 120 | | molecular models and density maps for journal articles and presentations. |
| 121 | | You'll be introduced to the myriad of options to enhance your graphics: |
| 122 | | raytracing, depth cueing, supersampling, ribbon styles, silhouette edges, |
| 123 | | subdivision level, text labels, clipping and capping, transparent or |
| 124 | | colored backgrounds, fish eye views, presets, lighting, shininess, ambient |
| 125 | | occlusion, lenticular images, and movie transitions and encoding. You'll |
| 126 | | try these techniques on your own data (downloaded or brought on a flash |
| 127 | | drive or CD) or using example data we provide. |
| 128 | | Participants should have familiarity with Chimera. |
| 129 | | - Tom Goddard, Greg Couch |
| 130 | | |
| 131 | | Jul 31, 2008 |
| 132 | | 30-minute demonstration of Cytoscape and Chimera to ~12 undergraduate |
| 133 | | students (from the organic chemistry class, University of San Francisco) |
| 134 | | - Scooter Morris, Elaine Meng |
| 135 | | |
| 136 | | Aug 5, 2008 |
| 137 | | UCSF Library Course: Customizing and tailoring UCSF Chimera, |
| 138 | | 9-11am at the UCSF Mission Bay Campus. |
| 139 | | |
| 140 | | This session will cover methods for customizing Chimera for specific tasks |
| 141 | | and projects. Topics include: writing command and demonstration scripts; |
| 142 | | accessing Chimera data structures using Python; adding per-frame actions |
| 143 | | for analyzing molecular dynamics; adding user-defined headers to sequence |
| 144 | | alignment displays; adding your own user interface elements to Chimera. |
| 145 | | |
| 146 | | Participants should have hands-on experience with Chimera. If there is a |
| 147 | | topic of particular interest, please mention it at the beginning of the |
| 148 | | session (or contact us beforehand) to ensure that we allot sufficient |
| 149 | | time to go into details. |
| 150 | | - Eric Pettersen, Conrad Huang |
| 151 | | |
| 152 | | Aug 19, 2008 |
| 153 | | UCSF Library Course: Exploring Protein Similarity Networks |
| 154 | | with Cytoscape, UCSF Chimera, and SFLD, |
| 155 | | 9-11am at the UCSF Mission Bay Campus. |
| 156 | | |
| 157 | | This session will cover the tools and methods that have been developed |
| 158 | | at UCSF to explore protein similarity networks. A protein similarity |
| 159 | | network is a network of nodes and edges where the nodes represent the |
| 160 | | protein and the edges represent some measure of the similarity between |
| 161 | | the proteins. These networks have been used to infer functional and |
| 162 | | evolutionary relationships between proteins and protein families. |
| 163 | | Session topics include: overview of protein similarity networks; using |
| 164 | | SFLDLoader to import protein similarity networks into Cytoscape; |
| 165 | | Cytoscape layouts suitable for protein similarity networks; exploring |
| 166 | | structural relationships with structureViz; creating your own protein |
| 167 | | similarity network; adding structural annotation to an existing protein |
| 168 | | similarity network. |
| 169 | | |
| 170 | | Participants should be familiar with Cytoscape and have some experience |
| 171 | | with molecular structure visualization. |
| 172 | | - Scooter Morris |
| 173 | | |
| 174 | | Nov 13, 2008 |
| 175 | | NCRR PI meeting, Bethesda, Maryland. Presentation of multiscale modeling |
| 176 | | capabilities with animations of rat brain data and Space Navigator use. |
| 177 | | - Tom Ferrin |
| 178 | | |
| 179 | | Dec 2008 |
| 180 | | Chimera used to create image for RBVI holiday card: 20S Proteasome. |
| 181 | | - Tom Goddard, Scooter Morris |
| 182 | | |
| 183 | | Dec 11, 2008 |
| 184 | | Demonstration for donors to the UCSF School of Pharmacy: |
| 185 | | visualization of the 8-segment RNA genome of influenza virus in |
| 186 | | electron microscope tomography data using special-purpose hardware: |
| 187 | | LCD stereo glasses, Space Navigator 6-degree-of-freedom input device, |
| 188 | | and Phantom force feedback input device for marker placement. |
| 189 | | - Tom Goddard |
| 190 | | |
| 191 | | Dec 12, 2008 |
| 192 | | Chimera Release Party and RBVI Open House (2-5 pm in Genentech Hall N453, |
| 193 | | UCSF Mission Bay) for local researchers and students to meet the Chimera |
| 194 | | developers, see demonstrations and discuss features, tour the RBVI |
| 195 | | facilities, and share refreshments. |
| 196 | | - Chimera team |
| 197 | | |
| 198 | | Jan 14, 2009 |
| 199 | | Chimera demonstrations for high school students participating in the |
| 200 | | Science and Education Partnership. Two half-hour demonstrations |
| 201 | | to Lincoln High School students and instructors (~18 total): |
| 202 | | viewing a nanomachine and the enzyme mevalonate kinase in stereo. |
| 203 | | - Tom Goddard |
| 204 | | |
| 205 | | Jan 23, 2009 |
| 206 | | Talk on electron tomography for Automated Molecular Imaging forum, |
| 207 | | Scripps Research Institute. |
| 208 | | - Tom Goddard |
| 209 | | |
| 210 | | Feb 1, 2009 |
| 211 | | Talk on electron tomography entitled "Interactive Visualization of Spikes, |
| 212 | | Pores and Cages" at Asia-Pacific Congress on Electron Tomography, |
| 213 | | Brisbane, Australia. http://homepage.mac.com/marshbj/APCET/ |
| 214 | | - Tom Goddard |
| 215 | | |
| 216 | | Feb 28, 2009 |
| 217 | | Presentation on current AMBER-related Chimera features and plans for |
| 218 | | further development at the AMBER developers' meeting (Feb 28-Mar 1), |
| 219 | | St. Simons Island, Georgia. |
| 220 | | - Eric Pettersen |
| 221 | | |
| 222 | | Mar 1-3, 2009 |
| 223 | | Biophysical Society 2009, Boston: |
| 224 | | RBVI staff will be giving several presentations at the NIH NCRR |
| 225 | | exhibitor booth. http://www.cgl.ucsf.edu/events/BPS2009/ |
| 226 | | - Scooter Morris, Tom Goddard |
| 227 | | |
| 228 | | Mar 31, 2009 |
| 229 | | UCSF Library Course: |
| 230 | | Molecular Visualization with UCSF Chimera: Getting Started |
| 231 | | 10am-noon at the UCSF Mission Bay Campus. |
| 232 | | http://www.library.ucsf.edu/help/classes |
| 233 | | |
| 234 | | This session will go over the basics of molecular visualization and provide |
| 235 | | an introduction to how to use UCSF Chimera to load, visualize, and manipulate |
| 236 | | protein structures. Topics include: installing Chimera; Chimera menus and |
| 237 | | commands; fetching protein structures from ModBase and PDB; selections and |
| 238 | | actions; depictions; key tools: Side View, Model Panel, and the Selection |
| 239 | | Panel; and saving your work: sessions and images. Participants should have |
| 240 | | a basic understanding of protein structure. All participants will receive |
| 241 | | a CD with UCSF Chimera installers for Mac, PC, and Linux as well as |
| 242 | | example files and additional exercises. |
| 243 | | - Scooter Morris, Conrad Huang |
| 244 | | |
| 245 | | Apr 7, 2009 |
| 246 | | UCSF Library Course: |
| 247 | | Analyzing and Comparing Structures with UCSF Chimera |
| 248 | | 10am-noon at the UCSF Mission Bay Campus. |
| 249 | | |
| 250 | | This session will cover general structure visualization and analysis |
| 251 | | with Chimera. Topics include: identifying hydrogen bonds and contacts; |
| 252 | | measuring distances and angles, rotating bonds; viewing amino acid |
| 253 | | sidechain rotamers, "mutating" residues; displaying attributes such as |
| 254 | | B-factor and residue hydrophobicity; superimposing and comparing |
| 255 | | structures, morphing; viewing NMR ensembles and molecular dynamics |
| 256 | | trajectories. Participants should have some knowledge of protein structure |
| 257 | | and molecular graphics, but expertise with Chimera is not required. |
| 258 | | Participants are encouraged to bring structures of interest (or note their |
| 259 | | Protein Data Bank IDs for retrieval in the lab) as "real-life" examples |
| 260 | | to supplement the provided exercises. |
| 261 | | - Elaine Meng |
| 262 | | |
| 263 | | Apr 14, 2009 |
| 264 | | UCSF Library Course: |
| 265 | | Exploring Sequence-Structure Relationships with UCSF Chimera |
| 266 | | 10am-noon at the UCSF Mission Bay Campus. |
| 267 | | |
| 268 | | This session will cover working with protein sequence alignments together |
| 269 | | with 3D structures. Topics include: customizing alignment appearance |
| 270 | | in Chimera; sequence-structure association and crosstalk; calculating |
| 271 | | sequence conservation and displaying it on structures; matching |
| 272 | | (superimposing) structures using an alignment; comparing structures of |
| 273 | | the same protein or different but related proteins; creating sequence |
| 274 | | alignments in Chimera; other sources of sequence alignments. Participants |
| 275 | | should have some experience using Chimera and a basic understanding of |
| 276 | | protein structure and sequence alignments. Participants are encouraged |
| 277 | | to bring data on systems of interest (structure IDs, and if available, |
| 278 | | corresponding sequence alignments) as "real-life" examples to supplement |
| 279 | | the provided exercises. |
| 280 | | - Elaine Meng, Eric Pettersen |
| 281 | | |
| 282 | | Apr 21, 2009 |
| 283 | | UCSF Library Course: |
| 284 | | Electron Microscope Tomography in UCSF Chimera |
| 285 | | 10am-noon at the UCSF Mission Bay Campus. |
| 286 | | |
| 287 | | This course provides hands-on training exploring electron tomography maps. |
| 288 | | You'll learn to trace membranes, mask and extract structures, filter |
| 289 | | data, and fit models in cellular tomograms containing microtubules, |
| 290 | | nuclear pores, vesicles and assorted subcellular structures. Chimera map |
| 291 | | capabilities are also useful for single-particle EM reconstructions |
| 292 | | and x-ray crystallography maps and researchers studying those data types |
| 293 | | may benefit from this course. Participants should have familiarity with |
| 294 | | Chimera. |
| 295 | | - Tom Goddard |
| 296 | | |
| 297 | | Apr 28, 2009 |
| 298 | | UCSF Library Course: |
| 299 | | Publication-Quality Images with UCSF Chimera |
| 300 | | 10am-noon at the UCSF Mission Bay Campus. |
| 301 | | |
| 302 | | This session provides hands-on training for creating images for journal |
| 303 | | articles and presentations. You will be introduced to the myriad of options |
| 304 | | to enhance your graphics: presets, depth cueing, transparency, silhouette |
| 305 | | edges, lighting, shininess, text labels, clipping and capping, color |
| 306 | | choices, raytracing, supersampling, subdivision quality, transparent |
| 307 | | backgrounds for image compositing, ribbon styles, stereo pairs, fish eye |
| 308 | | views, ambient occlusion, and lenticular images. Then you'll try some of |
| 309 | | these techniques on your own data (downloaded or brought on a flash drive |
| 310 | | or CD) or with example data we provide. Participants should have |
| 311 | | familiarity with Chimera. |
| 312 | | - Greg Couch |
| 313 | | |
| 314 | | May 5, 2009 |
| 315 | | UCSF Library Course: |
| 316 | | Movie Making with UCSF Chimera |
| 317 | | 10am-noon at the UCSF Mission Bay Campus. |
| 318 | | |
| 319 | | This course provides hands-on training creating movies of molecular models |
| 320 | | for journal articles and presentations. You'll create an animation showing |
| 321 | | a ligand binding to a receptor with morphing between unbound and bound |
| 322 | | states. Titling, movie encoding, animation commands, and effects (edging |
| 323 | | highlighting, shininess, smoothing) will be covered. Participants should |
| 324 | | have familiarity with Chimera. |
| 325 | | - Tom Goddard |
| 326 | | |
| 327 | | May 12, 2009 |
| 328 | | UCSF Library Course: |
| 329 | | Customizing and Tailoring UCSF Chimera |
| 330 | | 10am-noon at the UCSF Mission Bay Campus. |
| 331 | | |
| 332 | | This session will cover methods for customizing Chimera for specific tasks |
| 333 | | and projects, writing command scripts, and programming Chimera in Python. |
| 334 | | Other topics will be covered based on attendees interests. Please send |
| 335 | | mail to chimera-dev-classcgl.ucsf.edu if you would like to see a specific |
| 336 | | topic covered in depth. Possible topics include (but are not limited to): |
| 337 | | per-frame scripting of molecular dynamics trajectories; using the Demo |
| 338 | | Editor; adding user-defined headers to sequence alignments; writing |
| 339 | | Chimera extensions or commands. Participants should have hands-on |
| 340 | | experience with Chimera. |
| 341 | | - Eric Pettersen, Conrad Huang |
| 342 | | |