| | 1 | --------------------------------------------------------------------------- |
| | 2 | 2010/2011 report |
| | 3 | --------------------------------------------------------------------------- |
| | 4 | |
| | 5 | June 2010 - March 2011 |
| | 6 | Created 23 [http://www.cgl.ucsf.edu/chimera/videodoc/videodoc.html video tutorials] |
| | 7 | demonstrating different analysis techniques of electron microscopy density maps and |
| | 8 | molecular models using UCSF Chimera. Most videos are 5 to 10 minute long computer |
| | 9 | screen captures with audio narration showing step-by-step analysis. Each was made to |
| | 10 | address specific questions posed by users. Streaming videos are distributed via the |
| | 11 | RBVI web site using an in-browser Flash movie player. Total preparation time 50 hours. |
| | 12 | - Tom Goddard |
| | 13 | |
| | 14 | June 9, 2010 and July 7, 2010 and July 21, 2010 |
| | 15 | Demonstration showing DNA for total of 38 students ages 8-12 from |
| | 16 | [http://www.celsiusandbeyond.com/ Celsius and Beyond] |
| | 17 | summer science camp on 3 separate days. |
| | 18 | Organized with Auritte Cohen-Ross. Showed light |
| | 19 | microscopy volume time series of chromosomes in dividing cells, electron |
| | 20 | microscopy of chromosomes, DNA molecular structure, nucleosomes and chromatin |
| | 21 | (http://www.cgl.ucsf.edu/chimera/dna-jun2010/dnademo.html). |
| | 22 | 8 hours preparation. 1 hour demonstration in RBVI visualization room. |
| | 23 | Many questions from kids "What happens if you eat a bubble?". |
| | 24 | - Tom Goddard |
| | 25 | |
| | 26 | June 16-17, 2010 |
| | 27 | Summer School '10 : Python Applied to Computational Chemistry and Molecular Modelling, |
| | 28 | Universitat Autònoma de Barcelona, Spain. ~50 attendees. |
| | 29 | http://hpc.xrqtc.com/index.php?option=com_content&view=article&id=118:sm10-module-c&catid=54:recent-conference&Itemid=75 |
| | 30 | - Conrad Huang |
| | 31 | |
| | 32 | July 11, 2010 |
| | 33 | Presentation on Cytoscape at ISMB 2010 to an audience of approximately 60. |
| | 34 | http://www.iscb.org/cms_addon/conferences/ismb2010/technologytrack.php |
| | 35 | Boston, MA. |
| | 36 | The presentation provided an introduction to Cytoscape's capabilities and |
| | 37 | some of the new features in Cytoscape 2.7 along with some new and recently |
| | 38 | enhanced plugins. |
| | 39 | - Scooter Morris |
| | 40 | |
| | 41 | July 13, 2010 |
| | 42 | "Network visualization and analysis using Cytoscape" Birds of a Feather |
| | 43 | session at ISMB 2010. Scooter Morris facilitated this session to solicit |
| | 44 | input from Cytoscape users and developers. About 30 participants. |
| | 45 | |
| | 46 | July 18, 2010 |
| | 47 | "Cytoscape User Tutorial". 2 hour introductory user tutorial given to about |
| | 48 | 50 users as part of the annual Cytoscape Retreat. |
| | 49 | - Scooter Morris |
| | 50 | |
| | 51 | July 18, 2010 |
| | 52 | Plugin demonstration on CyCommandHandlers as part of the Cytoscape Retreat to |
| | 53 | about 100 participants. University of Michigan, Ann Arbor, MI. |
| | 54 | - Scooter Morris |
| | 55 | |
| | 56 | July 22, 2010 |
| | 57 | Gave two 20 minute demonstrations to 24 students, part of [http://www.ucop.edu/cosmos/ COSMOS] science |
| | 58 | program. Organized by Ken Dill. Showed DNA atomic models, small duplex, nucleosomes, |
| | 59 | chromatin filament model, chromosomes seen in electron microscopy. Used RBVI visualization room. |
| | 60 | 8 hours preparation. |
| | 61 | - Tom Goddard |
| | 62 | |
| | 63 | August 16, 2010 |
| | 64 | Tutorial given at CSB 2010, Stanford: "Analysis and Visualization of Biological Networks". |
| | 65 | This was a 1/2 day tutorial given to 15 participants that covered visualization |
| | 66 | and analytical approaches to understanding biological networks. Second half of |
| | 67 | tutorial introduced Cytoscape as a tool and provided a hands-on exercise. |
| | 68 | - Scooter Morris |
| | 69 | |
| | 70 | August 16, 2010 |
| | 71 | 10 minute SFLD demo, followed by 2 hour SFLD/cytoscape tutorial. Attendees worked through |
| | 72 | exercises designed to give them basic familiarity with manipulating a cytoscape sequence |
| | 73 | and/or structure similarity network of their superfamily of choice, of the sort downloaded |
| | 74 | from the SFLD, to investigate questions of biological interest. |
| | 75 | Audience: ~25 grad students, postdocs, staff from the EFI. 15 hours preparation. |
| | 76 | - Shoshana Brown |
| | 77 | |
| | 78 | September 29, 2010 |
| | 79 | 40-minute presentation to ~14 students in UCSF BP204, "Macromolecular Interactions |
| | 80 | and Assemblies" class. Included 5 min on history of Computer Graphics Lab, the remainder |
| | 81 | a Chimera demonstration: basic usage, morphing, viewing sequences, showing attribute |
| | 82 | values, building multimers, stereo. Prep time 12 hours including updating the previous |
| | 83 | year's presentation, testing with the current version of Chimera, rehearsing. |
| | 84 | - Elaine Meng, Eric Pettersen |
| | 85 | |
| | 86 | September 29, 2010 |
| | 87 | 30-minute presentation to ~50 attendees, then followed by 90-minute tutorial to ~20 students at |
| | 88 | Department of Computational and System Biology, School of Medicine and Clinical Translational |
| | 89 | Science Institute, University of Pittsburgh. The presentation part, titled "Introduction to UCSF |
| | 90 | Chimera, an Extensible Molecular Modeling System", covered the brief history of molecular |
| | 91 | visualization, RBVI history and Chimera highlighted features. The tutorial part went over |
| | 92 | several basic examples in the "getting started" document. Prep time 40 hours. |
| | 93 | - YZ (Zheng Yang) |
| | 94 | |
| | 95 | October 12-13, 2010 |
| | 96 | Poster presentation on segmentation and measurement of bacteria in termite gut at NIH workshop |
| | 97 | "Recent Advances and Future Prospects for Visualizing Macromolecular Complexes and |
| | 98 | Cellular Structures". 170 participants. 12 hours preparation. |
| | 99 | Discussions of research projects with about 10 attendees |
| | 100 | https://conferences.niaid.nih.gov/vbbethesda/ |
| | 101 | - Tom Goddard |
| | 102 | |
| | 103 | October 25, 2010 |
| | 104 | Invited talk, "Annotation Error in Public Databases," at the 2010 Pathway Tools Workshop, |
| | 105 | Menlo Park, CA. http://bioinformatics.ai.sri.com/ptools10/agenda.html |
| | 106 | Discussed SFLD and using sequence similarity networks in Cytoscape. ~100 attendees. |
| | 107 | - Alexandra Schnoes |
| | 108 | |
| | 109 | October 27, 2010 |
| | 110 | 45-minute Chimera teaching demonstration to ~30 students for a graduate course in protein |
| | 111 | engineering at UC Berkeley (taught by Danielle Tullman-Ercek, Asst. Professor, Dept. of |
| | 112 | Chemical and Biomolecular Engineering, UC Berkeley). Covered basic usage, finding H-bonds, |
| | 113 | morphing, saving trajectories as movies, viewing sequences, showing attribute values, |
| | 114 | evaluating rotamers, performing virtual mutations, building multimers, and using stereo. |
| | 115 | Prep time 20 hours including adding more material to the previously developed demo, |
| | 116 | testing with the current version of Chimera, and rehearsing. |
| | 117 | - Elaine Meng, Eric Pettersen |
| | 118 | |
| | 119 | November 18, 2010 |
| | 120 | Two 45-minute demonstrations showing termite gut and OpcA meningitis bacterium protein to |
| | 121 | SMART team Lincoln high school students. This is a UCSF Science and Health Education |
| | 122 | Partnership (SEP) program. Showed FIBSEM data, spirochetes, flagella, handedness, |
| | 123 | endo-flagella, protein morphing, hydrophobic and electrostatic surface coloring to identify |
| | 124 | transmembrane and binding regions. 16 students. 6 hours preparation. |
| | 125 | - Tom Goddard |
| | 126 | |
| | 127 | December 2010 |
| | 128 | Chimera used to create image for RBVI holiday card: glutamine synthetase 12-mer styled as an |
| | 129 | "architectural plan" (black outlines around transparent ribbons on aged parchment background). Estimated 36 person-hours of work (including preparing candidate images, voting, format converting). |
| | 130 | - YZ (Zheng Yang) |
| | 131 | |
| | 132 | December 2, 2010 |
| | 133 | Two 45-minute demonstrations showing termite gut and OpcA meningitis bacterium protein to |
| | 134 | SMART team Galileo high school students. This is a UCSF Science and Health Education Partnership (SEP) |
| | 135 | program. Showed FIBSEM data, spirochetes, flagella, handedness, endo-flagella, protein morphing, |
| | 136 | hydrophobic and electrostatic surface coloring to identify transmembrane and binding regions. |
| | 137 | 20 students. 4 hours preparation. |
| | 138 | - Tom Goddard |
| | 139 | |
| | 140 | December 8, 2010 |
| | 141 | Two hour discussion and Chimera demonstration of myosin thick filament regulation for |
| | 142 | UCSF Tetrad graduate program macromolecules discussion group. Looked at thick filament architecture and |
| | 143 | myosin 2 heavy and light chain atomic models using stereo 3d visualization. 5 graduate |
| | 144 | students, led by professor Zev Gartner. 8 hours preparation. |
| | 145 | - Tom Goddard |
| | 146 | |
| | 147 | December 13, 2010 |
| | 148 | Chimera Release Party and RBVI Open House (2-5 pm in Genentech Hall N453, |
| | 149 | UCSF Mission Bay) for local researchers and students to meet the Chimera |
| | 150 | developers, see demonstrations and discuss features, tour the RBVI |
| | 151 | facilities, and share refreshments. Estimated 50 attendees, 3 hours for the event, |
| | 152 | 24 person-hours preparation, publicity, and cleanup. |
| | 153 | - Chimera team |
| | 154 | |
| | 155 | January 12, 2010 |
| | 156 | Created 3 videos showing how to explore virus models for HHMI Viral Outbreak |
| | 157 | CDROM distribution to US high schools. Videos totaled 15 minutes and explained |
| | 158 | Chimera virus display, finding viruses structures on the web, and antibodies |
| | 159 | bound to West Nile virus. Expected distribution is several thousand CDs. |
| | 160 | Preparation time 15 hours. |
| | 161 | - Tom Goddard |
| | 162 | |
| | 163 | January 13, 2011 |
| | 164 | 20 minute SFLD demo, followed by 10 minute Q & A, showing how the SFLD can be used to get |
| | 165 | information about the biological context of an uncharacterized target of interest. |
| | 166 | Audience: ~20 EFI investigators and other staff. 15 hours preparation. |
| | 167 | - Shoshana Brown |
| | 168 | |
| | 169 | February 11, 2011 |
| | 170 | Demonstration showing immune system structures to 7 visiting candidate UCSF graduate |
| | 171 | students. West Nile virus with bound antibodies, cytotoxic T-cell EM data. Also |
| | 172 | showed 3d printer and plastic molecular models. Two 35 minute demonstrations. |
| | 173 | 4 hours preparation. |
| | 174 | - Tom Goddard |
| | 175 | |
| | 176 | February 11, 2011 |
| | 177 | Seminar at University of Toronto covering base cytoscape and RBVI plugins to group |
| | 178 | of about 50. The seminar was 2 hours and included introductory topics as well as |
| | 179 | some advanced discussion. |
| | 180 | 10 hours preparation |
| | 181 | - Scooter Morris |
| | 182 | |
| | 183 | February 18, 2011 |
| | 184 | Demonstration showing clathrin cages to 4 visiting candidate UCSF graduate |
| | 185 | students. Clathrin cage triskelions, molecular assembly model, animation. Also |
| | 186 | showed 3d printer and plastic molecular models. Two 35 minute demonstrations. |
| | 187 | 4 hours preparation. |
| | 188 | - Tom Goddard |
| | 189 | |
| | 190 | March 14-17, 2011 |
| | 191 | Chimera tutorial at [http://blake.bcm.edu/emanwiki/Ws2011/Agenda EMAN single particle reconstruction workshop], |
| | 192 | showing fitting atomic models in electron microscopy maps, symmetry, presentation images |
| | 193 | using Rous sarcoma virus data. Produced |
| | 194 | [http://www.cgl.ucsf.edu/chimera/videodoc/RousSarcomaVirus/ video version] of the workshop tutorial. |
| | 195 | Tutorial was 1 hour with ~100 participants at University of Houston. |
| | 196 | Answered approximately 30 Chimera usage questions during 4-day workshop |
| | 197 | and demonstrated features to individual workshop participants, about 10 hours total time. |
| | 198 | 20 hours preparation. |
| | 199 | - Tom Goddard |
| | 200 | |
| | 201 | March 19, 2011 |
| | 202 | Half day Cytoscape tutorial at [http://vizbi.org/2011/ VizBI 2011] to 20 participants, showing basic Cytoscape plus clusterMaker, structureViz, chemViz, and nodeCharts. This was followed by a half day workshop where participants brought their own data sets and collaborated on analyses and visualizations. |
| | 203 | |
| | 204 | 16 hours preparation |
| | 205 | - Scooter Morris |