[Chimera-users] Making Fusion Proteins using Chimera

[Tom Goddard]

Hi Milad,

An easy way to make a fusion protein is to use ChimeraX and run AlphaFold to predict the structure.  AlphaFold is not available in our old Chimera program -- no new features have gone into Chimera in over 5 years and many new capabilities are in ChimeraX.

In ChimeraX, use menu Tools / Structure Prediction / AlphaFold, paste in your fusion protein sequence and press Predict.  You may need to log into your Google account since the calculation is run on Google Colab servers.  Calculation time is 5 minutes to a few hours depending on sequence length.

	Tom


> On Aug 4, 2022, at 7:40 AM, Eric Pettersen via Chimera-users <chimera-users at cgl.ucsf.edu> wrote:
> 
> 
> 
>> Begin forwarded message:
>> 
>> From: Milad Mapar <milad.mapar at yahoo.com <mailto:milad.mapar at yahoo.com>>
>> Subject: Making Fusion Proteins using Chimera
>> Date: August 4, 2022 at 6:26:46 AM PDT
>> To: "pett at cgl.ucsf.edu <mailto:pett at cgl.ucsf.edu>" <pett at cgl.ucsf.edu <mailto:pett at cgl.ucsf.edu>>
>> 
>> Dear Eric
>> 
>> Hello
>> 
>> With thanks to your support on Chimera, could you please help me to make a Fusion Protein using Chimera 1.16
>> 
>> Any links to tutorials or hints would be appreciated 
>> 
>> Many thanks 
>> 
>> Pharmaceutical Biotechnology PhD Student
>> Milad 
> 
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