[Chimera-users] Save multiple fitted maps in Chimera

[Elaine Meng]

Dear Huabin,
You could save a Chimera session, but you'd also need to keep the input tomogram files and density map files in their current locations since the session file does not include them.
<https://www.rbvi.ucsf.edu/chimera/docs/UsersGuide/sessions.html>

For saving multiple maps to one file, I believe the only choice is "Chimera map format"
<https://www.rbvi.ucsf.edu/chimera/docs/UsersGuide/midas/volume.html#output>
<https://www.rbvi.ucsf.edu/chimera/docs/UsersGuide/midas/volume.html#chimap>

HOWEVER, as I understand it, simply saving a map to a file does not preserve any rotations and translations of that map such as from fitting.  So first you may need to resample each density map to create a new density map in the new orientation, as discussed here:
<https://www.rbvi.ucsf.edu/chimera/docs/UsersGuide/savemodel.html#afterfitting>

I hope this helps,
Elaine
-----
Elaine C. Meng, Ph.D.                       
UCSF Chimera(X) team
Department of Pharmaceutical Chemistry
University of California, San Francisco

> On Dec 13, 2021, at 11:59 AM, Huabin Zhou via Chimera-users <chimera-users at cgl.ucsf.edu> wrote:
> 
> Dear Chimera team and users,
> I am trying fit hundreds of protein density maps into a tomogram by manually adjusting their positions. After the placement, I found I can't save all the maps together in a single mrc file. Is there anyway to do so? Or is there a way to extract the relative orientations of the individual models?
> Thank you!
> Best,
> Huabin