[Chimera-users] Fc dimer fall apart after fusion with a GS linker

[Elaine Meng]

Hi TW,
My only guess is that these parts are originally in two different models and you are using the Join Models panel in Build Structure to form "C-N peptide bond."  In that case, make sure you are setting the "Move atoms on [...] side" option so that it moves the linker part, not the Fc part.  You said the linker is the part with "N" selected, so try:

Move atoms on [selected N atom] side

See the help:
<https://www.rbvi.ucsf.edu/chimera/docs/ContributedSoftware/editing/editing.html#join>

I hope this helps,
Elaine
-----
Elaine C. Meng, Ph.D.                       
UCSF Chimera(X) team
Department of Pharmaceutical Chemistry
University of California, San Francisco

> On Aug 15, 2021, at 4:47 PM, tiger wang via Chimera-users <chimera-users at cgl.ucsf.edu> wrote:
> 
> Dear Ms/Mr helper:
> I encounted an issue when I try to fuse one (G4S)x linker to one of the Fc "C" terminal using "join bond". Once selected the "C" atom on Fc C terminal and "N" atom on (G4S)x N terminal and hit "apply" button, the Fc dimer fall apart but the (G4S)x linker was connected to the C terminal of Fc. How to avoid the Fc dimer from falling apart? 
> Thanks very much for your help
> Sincerely 
> TW