[Chimera-users] resampling maps

[Jason Kaelber]

Hi all,

I decided to do a rough alignment of density maps manually and resample
onto a common coordinate system to feed the output mrc files into my fine
aligner. But, when I vop resample #1 onGrid #0, I get,
TypeError: Cannot cast ufunc add output from dtype('float32') to
dtype('uint8') with casting rule 'same_kind'

What might I be doing wrong to get this error? As far as I know, all the
maps should be uint8 since they were most recently operated on by David
Mastronarde's newstack command which outputs them as unsigned bytes.

Thanks,
Jason






<type 'exceptions.TypeError'> Exception in Tk callback
  Function: <bound method MidasUI.processCommand of <Midas.midas_ui.MidasUI
instance at 0x276b2d88>> (type: <type 'instancemethod'>)
  Args: (<Tkinter.Event instance at 0x276a2ea8>,)
  Event type: KeyPress (type num: 2)
Traceback (innermost last):
  File
"/opt/UCSF/Chimera64-1.8.1/lib/python2.7/site-packages/Pmw/Pmw_1_3/lib/PmwBase.py",
line 1747, in __call__
    return apply(self.func, args)
  File "/opt/UCSF/Chimera64-1.8.1/share/Midas/midas_ui.py", line 283, in
processCommand
    midas_text.makeCommand(cmdText)
  File "/opt/UCSF/Chimera64-1.8.1/share/Midas/midas_text.py", line 69, in
makeCommand
    f(c, args)
  File "/opt/UCSF/Chimera64-1.8.1/share/VolumeFilter/ChimeraExtension.py",
line 24, in vop_cmd
    vop_command(cmdname, args)
  File "/opt/UCSF/Chimera64-1.8.1/share/VolumeFilter/vopcommand.py", line
91, in vop_command
    doExtensionFunc(func, fargs, specInfo = spec)
  File "/opt/UCSF/Chimera64-1.8.1/share/Midas/midas_text.py", line 435, in
doExtensionFunc
    extFunc(*tuple(processedArgs), **kw)
  File "/opt/UCSF/Chimera64-1.8.1/share/VolumeFilter/vopcommand.py", line
553, in resample_op
    boundingGrid, False, None, modelId)
  File "/opt/UCSF/Chimera64-1.8.1/share/VolumeFilter/vopcommand.py", line
168, in add_operation
    scale = scale[i])
  File "/opt/UCSF/Chimera64-1.8.1/share/VolumeViewer/volume.py", line 1535,
in add_interpolated_values
    m[:,:,:] += values
<type 'exceptions.TypeError'>: Cannot cast ufunc add output from
dtype('float32') to dtype('uint8') with casting rule 'same_kind'

================================================
  Event contents:
    char:
    delta: 36
    height: ??
    keycode: 36
    keysym: Return
    keysym_num: 65293
    num: ??
    send_event: False
    serial: 39103
    state: 16
    time: 139539396
    type: 2
    widget: .59791048.661335648.802317088.802317376.802317448
    width: ??
    x: 159
    x_root: 524
    y: 9
    y_root: 988

TypeError: Cannot cast ufunc add output from dtype('float32') to
dtype('uint8') with casting rule 'same_kind'

  File "/opt/UCSF/Chimera64-1.8.1/share/VolumeViewer/volume.py", line 1535,
in add_interpolated_values
    m[:,:,:] += values

See reply log for Python traceback.
-- 
National Center for Macromolecular Imaging, N420
Baylor College of Medicine, MS BCM125
1 Baylor Plaza
Houston, TX, USA
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