[Chimera-users] how to reduce EM map resolution (Chimera 1.6.2)

[Sergey Ryazantsev]

  Hello
  I have low-resolution (~15A) model of decorated F-actin. I want to 
localize the "decoration" element by subtracting normal F-actin model 
obtained from Cryo-EM (EMD-5168) from my decorated model. The problem is 
that EMD-5168 has a higher resolution and "vop" command did not work.  
Even if it would work, it would be difficult to interpret the result of 
such subtraction. I think, both maps should be at approximately the same 
resolution.  I know how to create low-resolution map from PDB, but I 
could not find any solution how to lower the resolution of the EMD-5168.map

Any help would be greatly appreciated. Many thanks in advance. Sergey

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____________________________________________________________________

		     Sergey Ryazantsev Ph. D.
Electron Microscopy Laboratory (EMi)
Department of Biological Chemistry, David Geffen School of Medicine
University of California, Los Angeles.

Phone: (310) 825-1144 (office)  mailto:sryazant at ucla.edu