[Chimera-users] Intersurf

[Elaine Meng]

Hi George,
On the Intersurf dialog, click the red vertical bar on the histogram at the bottom to make it the "active" threshold, then click the color well (the square area next to the word "Color").  That brings up the Color Editor, in which you could check the Opacity option and move the "A" slider to introduce transparency.  Then on the Intersurf dialog, click Apply to apply the new coloring.

If you have a reasonably new Chimera it should be making compressed TIFF by default.  Older Chimera made uncompressed TIFF, much larger.   Personally I always use PNG which is generally quite small without loss of quality.
Elaine
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Elaine C. Meng, Ph.D. 
UCSF Computer Graphics Lab (Chimera team) and Babbitt Lab
Department of Pharmaceutical Chemistry
University of California, San Francisco

On Jan 24, 2012, at 9:22 AM, George Tzotzos wrote:

> 've used intersurf to show the interface area between two different protein sub-units. I'm attaching an image.
> 
> Is it possible to increase the transparency of the interface area shown in red? I found no way to do so.
> 
> Many thanks in advance for any suggestions
> 
> Regards
> 
> George
> 
> PS. No attachment as it is 33.5 Mb. By the way, is there a way to reduce the size of tiff images without compromising the quality?