[Chimera-users] select
Tom Goddard
goddard at sonic.net
Tue Oct 4 11:11:59 PDT 2011
Hi María,
To mask the virus particle to a spherical shell you would create the
spherical shell with the "shape sphere" command and do the masking with
the "mask" command. This will create ugly stair-step artifacts where
the density crosses the spherical surface and I don't have a good way to
eliminate those.
Here are the commands for EMDB 1123
open emd_1123.map
volume #0 originIndex 297 level 400
shape sphere radius 315 coord #0 slab 0,20 color pink
mask #0 #1
volume #2 step 1
and I've attached two pictures. The second command sets the center of
the origin of the map to the center of the virus and sets the contour
level. The shape command makes a spherical shell centered on the map
origin of radius 315 to 335. The mask command creates a copy of the map
setting density outside the spherical shell to 0. The last command
shows the resulting map at full resolution.
Details of all the commands are in the Chimera User's Guide. This
comes with Chimera. For example, you can use Chimera command
help shape
to show documentation in a web browser for the shape command.
Tom
>
> Hello,
>
> I working about Bam35c virus (emd_1123.map). That virus has a capsid and other things inside. I want used only exterior part (between 315 and 335 amstrons) and discard other parts. How can I select only this external part?
>
> Thank you for your help
>
> María
>
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> Chimera-users at cgl.ucsf.edu
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