[Chimera-users] creating a symmetric tetramer from a PDB file for a monomer?

[Thomas Goddard]

Hi Gea-Ny,

  Elaine's comments are right.  But there might be an easier way to
generate the potassium channel tetramer.  If the tetramer appears in
the crystal from which the monomer structure was obtained you can
use the Chimera Unit Cell or Multiscale tools to get it.  Generating
the unit cell only requires the CRYST1 PDB record, a one line record
describing the crystal symmetry group and unit cell parameters.  That
line appears in all files from the PDB web site.  If the tetramer is
not found in the crystal then it may be a non-trivial task to determine
the correct 4 matrices to build a tetramer from a monomer.

  If your K channel monomer is deposited at the PDB I can investigate
if you send me the PDB identifier.

  Sorry for the delayed reply.  I was on vacation and at a workshop the
past week and a half.

   Tom