<html><head><meta http-equiv="Content-Type" content="text/html; charset=utf-8"></head><body style="word-wrap: break-word; -webkit-nbsp-mode: space; line-break: after-white-space;" class="">Hi Dominic,<div class=""><br class=""></div><div class=""> The ChimeraX Distances tools measures distances between atoms in an atomic structure. You have image data, not an atomic structure so that is why it will not report any distances.</div><div class=""><br class=""></div><div class=""> One way to proceed would be to place a marker on every connected surface piece in each of the two images, then measure distances between these centroid markers. There is code to place the markers using ChimeraX here</div><div class=""><br class=""></div><div class=""><span class="Apple-tab-span" style="white-space:pre"> </span><a href="https://rbvi.github.io/chimerax-recipes/mark_blobs/mark_blobs.html" class="">https://rbvi.github.io/chimerax-recipes/mark_blobs/mark_blobs.html</a></div><div class=""><br class=""></div><div class="">Markers are treated just like atoms in ChimeraX so the Distance tool would work. But you want all nearby distances so you might try the Contacts tool</div><div class=""><br class=""></div><div class=""><span class="Apple-tab-span" style="white-space:pre"> </span><a href="https://www.rbvi.ucsf.edu/chimerax/docs/user/tools/clashes.html" class="">https://www.rbvi.ucsf.edu/chimerax/docs/user/tools/clashes.html</a></div><div class=""><br class=""></div><div class="">That may not give you what you want. Very likely you would need to write some custom Python code to get exactly what you want. Probably you also want the volume enclosed in each blob. If you need tips on how to write that Python code I can advise if you give specifics of what you want.</div><div class=""><br class=""></div><div class=""><span class="Apple-tab-span" style="white-space:pre"> </span>Tom</div><div class=""><br class=""></div><div class=""><br class=""></div><div class=""><br class=""></div><div class=""><br class=""></div><div class=""><br class=""><div><br class=""><blockquote type="cite" class=""><div class="">On Oct 6, 2021, at 4:11 AM, BINGHAM Dominic via ChimeraX-users <<a href="mailto:chimerax-users@cgl.ucsf.edu" class="">chimerax-users@cgl.ucsf.edu</a>> wrote:</div><br class="Apple-interchange-newline"><div class="">
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Hello,
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<div class="">I have a problem with which I’m trying to use chimeraX to solve. I have some images of synapses with which I have super-resolved two proteins in 3D (so two images/channels). I want to quantify the colocalisation of a structure of one protein to
the blobs of the other protein within a certain distance. Shown below is the non-segmented map of the 2 images of proteins. So for example I would want to quantify the colocalisation of the red blobs within a certain distance of specific larger green blobs.
I want to then compare these between different conditions.</div>
<div class=""><img apple-inline="yes" id="9F568B8E-F867-4943-AD88-9EB6962E767E" class="" width="393" height="466" src="cid:5E6B5354-093F-44B8-BCD8-9C01AFDEAF72"></div>
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<div class="">I have segmented the images already using "segmentat map ». I have been trying to start with the « distance » tool to measure how far one blob of protein is from the structure (Image shown below of two selected structures in just the map of one
protein to practice).</div>
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<div class=""><img apple-inline="yes" id="EE71C387-D936-4118-867D-FC7407B2DC6A" class="" width="418" height="519" src="cid:ED6171EE-E03F-47C8-A3AE-81A7E5FC9177"></div>
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<div class="">However, this doesn’t seem to work functionally as no distance is shown when I try to perform the function. Also, this function may possibly be too basic for what Im trying to do. That said, any help on how to use this function would still be
greatly appreciated. </div>
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<div class="">Ultimately, I think the best thing for what I would like is to be able to select a specific structure and have a measurement of all the blobs of the other protein within a defined 3D radius of this structure, and then compare this value between
different conditions. I have been looking through the user manual and I can’t seem to find anything that will do something like this… I (and my PhD) would be monumentally grateful if you could possibly help me with this problem? Either by pointing me to the
correct function, or even a thread that has discussed a similar problem that I may have missed? </div>
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<div class="">Thanks and have a wonderful day,</div>
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<div class="">Dominic Bingham</div>
<div class="">PhD student at Aix-Marseille University </div>
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