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<p class="MsoNormal">Hello,<o:p></o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal">My name is Yunsik Kang, and I am a postdoc in Marc Freeman’s lab at the Vollum Institute.
<o:p></o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal">I would love to use ChimeraX to predict the structure of my protein of interest. I watched all the YouTube videos and tried to run the program.
<o:p></o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal">Unfortunately, my protein is 5005 amino acids in humans and 2958 aa in yeast. I get a message “<span style="font-size:10.5pt;font-family:"Courier New";color:#212121;background:white">Please use the full AlphaFold system for long sequences.”<o:p></o:p></span></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal">My question is what is the best way to approach this problem? Should I cut the protein in half and run the program? In one of the videos, it mentions after 700 aa it will have problems. Will it work if I get Colab-Pro? Or would the server
crash no matter what.<o:p></o:p></p>
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<p class="MsoNormal">I am not a structural biologist, but I hope the structure can help be predict me with my research. <o:p></o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal">Thank you,<o:p></o:p></p>
<p class="MsoNormal">Yunsik<o:p></o:p></p>
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<p class="MsoNormal"><o:p> </o:p></p>
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