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<div class="moz-cite-prefix">Hi, Stéphane,</div>
<div class="moz-cite-prefix"><br>
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<div class="moz-cite-prefix"> The pixel size is just a value in the
header of the MRC file, and you can change it with Relion:<br>
</div>
<div class="moz-cite-prefix"><br>
</div>
<div class="moz-cite-prefix">relion_image_handler --i input.mrc --o
output.mrc --force_header_angpix [new_value]</div>
<div class="moz-cite-prefix"><br>
</div>
<div class="moz-cite-prefix">Best wishes,<br>
Vadim Kotov<br>
Postdoctoral fellow, Löw group<br>
EMBL Hamburg<br>
</div>
<div class="moz-cite-prefix"><br>
</div>
<div class="moz-cite-prefix"><br>
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<div class="moz-cite-prefix">On 17.06.21 10:07, Stéphane ROCHE via
ChimeraX-users wrote:<br>
</div>
<blockquote type="cite"
cite="mid:005b01d7634f$dd777a40$98666ec0$@i2bc.paris-saclay.fr">
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<p class="MsoNormal">Dear Mr/Mrs,<o:p></o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal">I am new to ChimeraX (v 0.7) and I have a
trouble when saving a map.<o:p></o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal">I am working with a .ccp4 map from an
electron microscopy project. I would like to slightly change
the pixel size that was inaccurately estimated during data
processing and chimeraX seems to be the program for doing
that. <o:p></o:p></p>
<p class="MsoListParagraph"
style="text-indent:-18.0pt;mso-list:l0 level1 lfo1"><!--[if !supportLists]--><span
style="mso-list:Ignore">-<span style="font:7.0pt "Times
New Roman""> </span></span><!--[endif]-->Opening
the map is fine.<o:p></o:p></p>
<p class="MsoListParagraph"
style="text-indent:-18.0pt;mso-list:l0 level1 lfo1"><!--[if !supportLists]--><span
style="mso-list:Ignore">-<span style="font:7.0pt "Times
New Roman""> </span></span><!--[endif]-->Then
I change the pixel voxelsize which is also fine.<o:p></o:p></p>
<p class="MsoListParagraph"
style="text-indent:-18.0pt;mso-list:l0 level1 lfo1"><!--[if !supportLists]--><span
style="mso-list:Ignore">-<span style="font:7.0pt "Times
New Roman""> </span></span><!--[endif]-->I
then save the modified map (I use the .mrc format here given
that I do not find a .ccp4 format).<o:p></o:p></p>
<p class="MsoListParagraph"
style="text-indent:-18.0pt;mso-list:l0 level1 lfo1"><!--[if !supportLists]--><span
style="mso-list:Ignore">-<span style="font:7.0pt "Times
New Roman""> </span></span><!--[endif]-->However
if I then use the map with my usual softwares (phenix, coot)
it looks like pure noise. But the map looks normal with some
other softwares (e.g. pymol). I also have no problem usually
with my .mrc maps with any software so I would assume the
problem is with the .mrc map I created<o:p></o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal">Do you know what I could do to solve this
problem ?<o:p></o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal">Thanks, <o:p></o:p></p>
<p class="MsoNormal">Stephane Roche<o:p></o:p></p>
<p class="MsoNormal">Institut de Biologie Intégrative de la
Cellule<o:p></o:p></p>
<p class="MsoNormal">91198 Gif sur Yvette<o:p></o:p></p>
<p class="MsoNormal">France<o:p></o:p></p>
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