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</o:shapelayout></xml><![endif]--></head><body lang=ZH-TW link="#0563C1" vlink="#954F72" style='text-justify-trim:punctuation'><div class=WordSection1><p class=MsoNormal><span lang=EN-US>Hi,<o:p></o:p></span></p><p class=MsoNormal style='mso-margin-top-alt:auto;mso-margin-bottom-alt:auto;background:white'><span lang=EN-US>I am trying to use Chimera X to display an amino acid with dual side chain conformations (PDB code: 7a5v) together with its cryoEM density map (</span><strong><span lang=EN-US style='font-size:10.5pt;font-family:"Helvetica",sans-serif;color:#333333'>EMDataResource: </span></strong><span lang=EN-US style='font-size:10.5pt;font-family:"Helvetica",sans-serif;color:#333333'><a href="https://www.emdataresource.org/EMD-11657" target="_blank"><span style='color:#337AB7'>EMD-11657</span></a>). </span><span lang=EN-US><o:p></o:p></span></p><p class=MsoNormal style='mso-margin-top-alt:auto;mso-margin-bottom-alt:auto;background:white'><span lang=EN-US>For example, Tyr 66 have two side chain conformations, however using the command below, it only showed one conformation.<o:p></o:p></span></p><p class=MsoNormal><span lang=EN-US>resfit /a:66 map #1 (a very nice command, by the way)<o:p></o:p></span></p><p class=MsoNormal><span lang=EN-US><o:p> </o:p></span></p><p class=MsoNormal><span lang=EN-US>I then used Chimera 1.15 to view the PDB coordinate, and it showed two side chain conformations for Tyr66 (as I saw in PyMol); on the other hand, Chimera X only shows a single conformation.<o:p></o:p></span></p><p class=MsoNormal><span lang=EN-US>Is there any way to display the two alternative conformations using Chimera X?<o:p></o:p></span></p><p class=MsoNormal><span lang=EN-US><o:p> </o:p></span></p><p class=MsoNormal><span lang=EN-US>Thanks a lot for your time, and possible help!<o:p></o:p></span></p><p class=MsoNormal><span lang=EN-US><o:p> </o:p></span></p><div style='mso-element:para-border-div;border:none;border-bottom:dotted windowtext 3.0pt;padding:0cm 0cm 1.0pt 0cm'><p class=MsoNormal style='border:none;padding:0cm'><span lang=EN-US>Best wishes,</span><span lang=EN-US><br><br>Winston<o:p></o:p></span></p><p class=MsoNormal style='border:none;padding:0cm'><span lang=EN-US><br><br><o:p></o:p></span></p></div><p class=MsoPlainText><span lang=EN-US><o:p> </o:p></span></p><p class=MsoPlainText><span lang=EN-US>*************************<o:p></o:p></span></p><p class=MsoPlainText><span lang=EN-US>ATOM 495 N ATYR A 66 124.799 162.717 112.222 0.50 11.67<o:p></o:p></span></p><p class=MsoPlainText><span lang=EN-US>N <o:p></o:p></span></p><p class=MsoPlainText><span lang=EN-US>ATOM 496 N BTYR A 66 125.017 162.760 112.080 0.50 12.99<o:p></o:p></span></p><p class=MsoPlainText><span lang=EN-US>N <o:p></o:p></span></p><p class=MsoPlainText><span lang=EN-US>ATOM 497 CA ATYR A 66 124.008 162.914 110.998 0.50 12.05<o:p></o:p></span></p><p class=MsoPlainText><span lang=EN-US>C <o:p></o:p></span></p><p class=MsoPlainText><span lang=EN-US>ATOM 498 CA BTYR A 66 124.035 162.916 111.002 0.50 14.20<o:p></o:p></span></p><p class=MsoPlainText><span lang=EN-US>C <o:p></o:p></span></p><p class=MsoPlainText><span lang=EN-US>ATOM 499 C ATYR A 66 124.638 164.062 110.190 0.50 11.84<o:p></o:p></span></p><p class=MsoPlainText><span lang=EN-US>C <o:p></o:p></span></p><p class=MsoPlainText><span lang=EN-US>ATOM 500 C BTYR A 66 124.433 164.115 110.140 0.50 13.40<o:p></o:p></span></p><p class=MsoPlainText><span lang=EN-US>C <o:p></o:p></span></p><p class=MsoPlainText><span lang=EN-US>ATOM 501 O ATYR A 66 125.008 165.077 110.788 0.50 11.86<o:p></o:p></span></p><p class=MsoPlainText><span lang=EN-US>O <o:p></o:p></span></p><p class=MsoPlainText><span lang=EN-US>ATOM 502 O BTYR A 66 124.411 165.246 110.660 0.50 14.99<o:p></o:p></span></p><p class=MsoPlainText><span lang=EN-US>O <o:p></o:p></span></p><p class=MsoPlainText><span lang=EN-US>ATOM 503 CB ATYR A 66 122.562 163.249 111.355 0.50 13.02<o:p></o:p></span></p><p class=MsoPlainText><span lang=EN-US>C <o:p></o:p></span></p><p class=MsoPlainText><span lang=EN-US>ATOM 504 CB BTYR A 66 122.666 163.125 111.624 0.50 17.22<o:p></o:p></span></p><p class=MsoPlainText><span lang=EN-US>C <o:p></o:p></span></p><p class=MsoPlainText><span lang=EN-US>ATOM 505 CG ATYR A 66 121.759 162.139 111.997 0.50 14.56<o:p></o:p></span></p><p class=MsoPlainText><span lang=EN-US>C <o:p></o:p></span></p><p class=MsoPlainText><span lang=EN-US>ATOM 506 CG BTYR A 66 121.542 163.315 110.654 0.50 21.09<o:p></o:p></span></p><p class=MsoPlainText><span lang=EN-US>C <o:p></o:p></span></p><p class=MsoPlainText><span lang=EN-US>ATOM 507 CD1ATYR A 66 121.860 161.839 113.348 0.50 16.21<o:p></o:p></span></p><p class=MsoPlainText><span lang=EN-US>C <o:p></o:p></span></p><p class=MsoPlainText><span lang=EN-US>ATOM 508 CD1BTYR A 66 120.718 162.258 110.325 0.50 25.90<o:p></o:p></span></p><p class=MsoPlainText><span lang=EN-US>C <o:p></o:p></span></p><p class=MsoPlainText><span lang=EN-US>ATOM 509 CD2ATYR A 66 120.832 161.436 111.248 0.50 17.74<o:p></o:p></span></p><p class=MsoPlainText><span lang=EN-US>C <o:p></o:p></span></p><p class=MsoPlainText><span lang=EN-US>ATOM 510 CD2BTYR A 66 121.189 164.579 110.209 0.50 29.25<o:p></o:p></span></p><p class=MsoPlainText><span lang=EN-US>C <o:p></o:p></span></p><p class=MsoPlainText><span lang=EN-US>ATOM 511 CE1ATYR A 66 120.974 160.942 113.960 0.50 18.53<o:p></o:p></span></p><p class=MsoPlainText><span lang=EN-US>C <o:p></o:p></span></p><p class=MsoPlainText><span lang=EN-US>ATOM 512 CE1BTYR A 66 119.590 162.443 109.543 0.50 32.51<o:p></o:p></span></p><p class=MsoPlainText><span lang=EN-US>C <o:p></o:p></span></p><p class=MsoPlainText><span lang=EN-US>ATOM 513 CE2ATYR A 66 119.990 160.494 111.824 0.50 18.57<o:p></o:p></span></p><p class=MsoPlainText><span lang=EN-US>C <o:p></o:p></span></p><p class=MsoPlainText><span lang=EN-US>ATOM 514 CE2BTYR A 66 120.067 164.784 109.421 0.50 27.89<o:p></o:p></span></p><p class=MsoPlainText><span lang=EN-US>C <o:p></o:p></span></p><p class=MsoPlainText><span lang=EN-US>ATOM 515 CZ ATYR A 66 120.076 160.228 113.177 0.50 20.29<o:p></o:p></span></p><p class=MsoPlainText><span lang=EN-US>C <o:p></o:p></span></p><p class=MsoPlainText><span lang=EN-US>ATOM 516 CZ BTYR A 66 119.252 163.714 109.107 0.50 30.80<o:p></o:p></span></p><p class=MsoPlainText><span lang=EN-US>C <o:p></o:p></span></p><p class=MsoPlainText><span lang=EN-US>ATOM 517 OH ATYR A 66 119.241 159.287 113.722 0.50 24.21<o:p></o:p></span></p><p class=MsoPlainText><span lang=EN-US>O <o:p></o:p></span></p><p class=MsoPlainText><span lang=EN-US>ATOM 518 OH BTYR A 66 118.168 163.886 108.291 0.50 39.05 <o:p></o:p></span></p><p class=MsoPlainText><span lang=EN-US><o:p> </o:p></span></p><p class=MsoNormal><span lang=EN-US><o:p> </o:p></span></p><p class=MsoNormal><span lang=EN-US><o:p> </o:p></span></p><p class=MsoNormal><span lang=EN-US><o:p> </o:p></span></p></div></body></html>