<html><head><meta http-equiv="Content-Type" content="text/html; charset=us-ascii"></head><body style="word-wrap: break-word; -webkit-nbsp-mode: space; line-break: after-white-space;" class=""><div class=""><font color="#0f1419" face="-apple-system, BlinkMacSystemFont, Segoe UI, Roboto, Helvetica, Arial, sans-serif" class=""><span style="caret-color: rgb(15, 20, 25); font-size: 15px; white-space: pre-wrap;" class="">Alexis Rohou asked on Twitter</span></font></div><div class=""><font color="#0f1419" face="-apple-system, BlinkMacSystemFont, Segoe UI, Roboto, Helvetica, Arial, sans-serif" class=""><span style="caret-color: rgb(15, 20, 25); font-size: 15px; white-space: pre-wrap;" class=""><br class=""></span></font></div><span class="css-16my406 r-qvutc0 r-poiln3 css-901oao r-bcqeeo" style="border: 0px solid black; box-sizing: border-box; color: rgb(15, 20, 25); display: inline; font-stretch: inherit; font-size: 15px; line-height: inherit; font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", Roboto, Helvetica, Arial, sans-serif; margin: 0px; padding: 0px; white-space: pre-wrap; word-wrap: break-word; min-width: 0px; caret-color: rgb(15, 20, 25);"><span class="Apple-tab-span" style="white-space:pre"> </span>In </span><div class="r-xoduu5 css-1dbjc4n" style="-webkit-box-align: stretch; -webkit-box-direction: normal; -webkit-box-orient: vertical; align-items: stretch; border: 0px solid black; box-sizing: border-box; display: inline-flex; flex-basis: auto; flex-direction: column; flex-shrink: 0; margin: 0px; min-height: 0px; min-width: 0px; padding: 0px; position: relative; z-index: 0; caret-color: rgb(15, 20, 25); color: rgb(15, 20, 25); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", Roboto, Helvetica, Arial, sans-serif; font-size: 15px; white-space: pre-wrap;"><span class="r-18u37iz" style="-webkit-box-direction: normal; -webkit-box-orient: horizontal; flex-direction: row;"><a dir="ltr" href="https://twitter.com/UCSFChimeraX" role="link" class="css-18t94o4 css-16my406 r-1loqt21 r-qvutc0 css-4rbku5 r-bcqeeo css-901oao r-poiln3 r-1n1174f" style="color: rgb(27, 149, 224); font-family: inherit; font-size: inherit; font-style: inherit; font-variant-caps: inherit; font-stretch: inherit; line-height: inherit; list-style: none; margin: 0px; text-align: inherit; text-decoration: none; cursor: pointer; border: 0px solid black; box-sizing: border-box; display: inline; padding: 0px; white-space: inherit; word-wrap: break-word; min-width: 0px;">@UCSFChimeraX</a></span></div><span class="css-16my406 r-qvutc0 r-poiln3 css-901oao r-bcqeeo" style="border: 0px solid black; box-sizing: border-box; color: rgb(15, 20, 25); display: inline; font-stretch: inherit; font-size: 15px; line-height: inherit; font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", Roboto, Helvetica, Arial, sans-serif; margin: 0px; padding: 0px; white-space: pre-wrap; word-wrap: break-word; min-width: 0px; caret-color: rgb(15, 20, 25);"> 1.1.1 and </span><span class="r-18u37iz" style="-webkit-box-direction: normal; -webkit-box-orient: horizontal; flex-direction: row; caret-color: rgb(15, 20, 25); color: rgb(15, 20, 25); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", Roboto, Helvetica, Arial, sans-serif; font-size: 15px; white-space: pre-wrap;"><a dir="ltr" href="https://twitter.com/hashtag/Isolde?src=hashtag_click" role="link" class="css-18t94o4 css-16my406 r-1loqt21 r-qvutc0 css-4rbku5 r-bcqeeo css-901oao r-poiln3 r-1n1174f" style="color: rgb(27, 149, 224); font-family: inherit; font-size: inherit; font-style: inherit; font-variant-caps: inherit; font-stretch: inherit; line-height: inherit; list-style: none; margin: 0px; text-align: inherit; text-decoration: none; cursor: pointer; border: 0px solid black; box-sizing: border-box; display: inline; padding: 0px; white-space: inherit; word-wrap: break-word; min-width: 0px;">#Isolde</a></span><span class="css-16my406 r-qvutc0 r-poiln3 css-901oao r-bcqeeo" style="border: 0px solid black; box-sizing: border-box; color: rgb(15, 20, 25); display: inline; font-stretch: inherit; font-size: 15px; line-height: inherit; font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", Roboto, Helvetica, Arial, sans-serif; margin: 0px; padding: 0px; white-space: pre-wrap; word-wrap: break-word; min-width: 0px; caret-color: rgb(15, 20, 25);"> by </span><div class="r-xoduu5 css-1dbjc4n" style="-webkit-box-align: stretch; -webkit-box-direction: normal; -webkit-box-orient: vertical; align-items: stretch; border: 0px solid black; box-sizing: border-box; display: inline-flex; flex-basis: auto; flex-direction: column; flex-shrink: 0; margin: 0px; min-height: 0px; min-width: 0px; padding: 0px; position: relative; z-index: 0; caret-color: rgb(15, 20, 25); color: rgb(15, 20, 25); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", Roboto, Helvetica, Arial, sans-serif; font-size: 15px; white-space: pre-wrap;"><span class="r-18u37iz" style="-webkit-box-direction: normal; -webkit-box-orient: horizontal; flex-direction: row;"><a dir="ltr" href="https://twitter.com/CrollTristan" role="link" class="css-18t94o4 css-16my406 r-1loqt21 r-qvutc0 css-4rbku5 r-bcqeeo css-901oao r-poiln3 r-1n1174f" style="color: rgb(27, 149, 224); font-family: inherit; font-size: inherit; font-style: inherit; font-variant-caps: inherit; font-stretch: inherit; line-height: inherit; list-style: none; margin: 0px; text-align: inherit; text-decoration: none; cursor: pointer; border: 0px solid black; box-sizing: border-box; display: inline; padding: 0px; white-space: inherit; word-wrap: break-word; min-width: 0px;">@CrollTristan</a></span></div><span class="css-16my406 r-qvutc0 r-poiln3 css-901oao r-bcqeeo" style="border: 0px solid black; box-sizing: border-box; color: rgb(15, 20, 25); display: inline; font-stretch: inherit; font-size: 15px; line-height: inherit; font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", Roboto, Helvetica, Arial, sans-serif; margin: 0px; padding: 0px; white-space: pre-wrap; word-wrap: break-word; min-width: 0px; caret-color: rgb(15, 20, 25);"> is there an easy way to add aa residues to the end of a chain?</span><div class=""><span class="css-16my406 r-qvutc0 r-poiln3 css-901oao r-bcqeeo" style="border: 0px solid black; box-sizing: border-box; color: rgb(15, 20, 25); display: inline; font-stretch: inherit; font-size: 15px; line-height: inherit; font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", Roboto, Helvetica, Arial, sans-serif; margin: 0px; padding: 0px; white-space: pre-wrap; word-wrap: break-word; min-width: 0px; caret-color: rgb(15, 20, 25);"><br class=""></span></div><div class=""><span class="css-16my406 r-qvutc0 r-poiln3 css-901oao r-bcqeeo" style="border: 0px solid black; box-sizing: border-box; display: inline; font-stretch: inherit; line-height: inherit; margin: 0px; padding: 0px; word-wrap: break-word; min-width: 0px; caret-color: rgb(15, 20, 25); font-size: 15px; white-space: pre-wrap;"><font color="#0f1419" face="-apple-system, BlinkMacSystemFont, Segoe UI, Roboto, Helvetica, Arial, sans-serif" class=""><a href="https://twitter.com/AlexisRohou/status/1386171500604977152" class=""><span class="Apple-tab-span" style="white-space:pre"> </span>https://twitter.com/AlexisRohou/status/1386171500604977152</a></font></span></div><div class=""><span class="css-16my406 r-qvutc0 r-poiln3 css-901oao r-bcqeeo" style="border: 0px solid black; box-sizing: border-box; display: inline; font-stretch: inherit; line-height: inherit; margin: 0px; padding: 0px; word-wrap: break-word; min-width: 0px; caret-color: rgb(15, 20, 25); font-size: 15px; white-space: pre-wrap;"><font color="#0f1419" face="-apple-system, BlinkMacSystemFont, Segoe UI, Roboto, Helvetica, Arial, sans-serif" class=""><br class=""></font></span></div><div class=""><span class="css-16my406 r-qvutc0 r-poiln3 css-901oao r-bcqeeo" style="border: 0px solid black; box-sizing: border-box; display: inline; font-stretch: inherit; line-height: inherit; margin: 0px; padding: 0px; word-wrap: break-word; min-width: 0px; caret-color: rgb(15, 20, 25); font-size: 15px; white-space: pre-wrap;"><font color="#0f1419" face="-apple-system, BlinkMacSystemFont, Segoe UI, Roboto, Helvetica, Arial, sans-serif" class="">Elaine says:</font></span></div><div class=""><span class="css-16my406 r-qvutc0 r-poiln3 css-901oao r-bcqeeo" style="border: 0px solid black; box-sizing: border-box; display: inline; font-stretch: inherit; line-height: inherit; margin: 0px; padding: 0px; word-wrap: break-word; min-width: 0px; caret-color: rgb(15, 20, 25); font-size: 15px; white-space: pre-wrap;"><font color="#0f1419" face="-apple-system, BlinkMacSystemFont, Segoe UI, Roboto, Helvetica, Arial, sans-serif" class=""><br class=""></font></span></div><div class=""><span class="css-16my406 r-qvutc0 r-poiln3 css-901oao r-bcqeeo" style="border: 0px solid black; box-sizing: border-box; display: inline; font-stretch: inherit; line-height: inherit; margin: 0px; padding: 0px; word-wrap: break-word; min-width: 0px; caret-color: rgb(15, 20, 25); white-space: pre-wrap; font-size: 15px;"><span style="caret-color: rgb(0, 0, 0); white-space: normal;" class="">There isn't an "add amino acid" yet in ChimeraX, only Chimera.... unless ISOLDE can do it (I don't know). So maybe it's an ISOLDE question.</span><br style="caret-color: rgb(0, 0, 0); white-space: normal;" class=""><br style="caret-color: rgb(0, 0, 0); white-space: normal;" class=""><span style="caret-color: rgb(0, 0, 0); white-space: normal;" class="">ChimeraX itself has building peptides from scratch but not sticking them on to the end of an existing peptide.</span><br style="caret-color: rgb(0, 0, 0); white-space: normal;" class=""><br style="caret-color: rgb(0, 0, 0); white-space: normal;" class=""><span style="caret-color: rgb(0, 0, 0); white-space: normal;" class="">Build Structure, Start Structure: peptide</span><br style="caret-color: rgb(0, 0, 0); white-space: normal;" class=""><span style="caret-color: rgb(0, 0, 0); white-space: normal;" class=""><</span><a href="https://rbvi.ucsf.edu/chimerax/docs/user/tools/buildstructure.html#start" style="white-space: normal;" class="">https://rbvi.ucsf.edu/chimerax/docs/user/tools/buildstructure.html#start</a><span style="caret-color: rgb(0, 0, 0); white-space: normal;" class="">></span><br style="caret-color: rgb(0, 0, 0); white-space: normal;" class=""><br style="caret-color: rgb(0, 0, 0); white-space: normal;" class=""><span style="caret-color: rgb(0, 0, 0); white-space: normal;" class="">or command "build start peptide"</span><br style="caret-color: rgb(0, 0, 0); white-space: normal;" class=""><span style="caret-color: rgb(0, 0, 0); white-space: normal;" class=""><</span><a href="https://rbvi.ucsf.edu/chimerax/docs/user/commands/build.html#start" style="white-space: normal;" class="">https://rbvi.ucsf.edu/chimerax/docs/user/commands/build.html#start</a><span style="caret-color: rgb(0, 0, 0); white-space: normal;" class="">></span><br style="caret-color: rgb(0, 0, 0); white-space: normal;" class=""><br style="caret-color: rgb(0, 0, 0); white-space: normal;" class=""><span style="caret-color: rgb(0, 0, 0); white-space: normal;" class="">Elaine</span></span></div></body></html>