<div dir="ltr">Hi all,<div><br></div><div>I am following the coloring sequence by <a href="https://www.rbvi.ucsf.edu/chimerax/data/conservation-coloring/conservation-coloring.html">conservation tutorial</a> but ran into some unexpected behavior. I used the the exact commands, but when I ran the "<font face="monospace">color byattribute seq_conservation</font>" command, I got an error, "<font face="monospace" color="#ff0000">No known/registered attribute seq_conservation</font>." The association was successful so I don't know why the seq_conservation attribute is not being set. See below for the log of my commands. </div><div><br></div><div>I also tried using my own structure and msa but ran into the same "No known / registered attribute seq_conservation" issue, despite associating with 0 mismatches. </div><div><br></div><div>Am I somehow missing something, or is something wrong? I am using a version of ChimeraX downloaded in November 2020, running on Windows 10. </div><div><br></div><blockquote class="gmail_quote" style="margin:0px 0px 0px 0.8ex;border-left:1px solid rgb(204,204,204);padding-left:1ex"><br><b>open 121p</b> </blockquote><blockquote class="gmail_quote" style="margin:0px 0px 0px 0.8ex;border-left:1px solid rgb(204,204,204);padding-left:1ex"><b>121p title:</b><br>Struktur und guanosintriphosphat-hydrolysemechanismus des C-terminal verkuerzten menschlichen krebsproteins P21-H-ras [more info...]<br><br>Chain information for 121p #1<br>Chain       Description<br>A  H-ras P21 protein<br>Non-standard residues in 121p #1<br>GCP — phosphomethylphosphonic acid guanylate ester<br>MG — magnesium ion<br>121p mmCIF Assemblies<br>1       author_defined_assembly<br><br><b>hide solvent</b><br><b>style ligand ball</b> </blockquote><blockquote class="gmail_quote" style="margin:0px 0px 0px 0.8ex;border-left:1px solid rgb(204,204,204);padding-left:1ex">Changed 32 atom styles<br><b>open <a href="https://www.rbvi.ucsf.edu/chimerax/data/conservation-coloring/121p-consurf.aln">https://www.rbvi.ucsf.edu/chimerax/data/conservation-coloring/121p-consurf.aln</a></b><br>Summary of feedback from opening //<a href="http://www.rbvi.ucsf.edu/chimerax/data/conservation-coloring/121p-consurf.aln">www.rbvi.ucsf.edu/chimerax/data/conservation-coloring/121p-consurf.aln</a> </blockquote><blockquote class="gmail_quote" style="margin:0px 0px 0px 0.8ex;border-left:1px solid rgb(204,204,204);padding-left:1ex">fetched from https<br>notes Fetching 121p-consurf(1).aln from <a href="https://www.rbvi.ucsf.edu/chimerax/data/conservation-coloring/121p-consurf.aln">https://www.rbvi.ucsf.edu/chimerax/data/conservation-coloring/121p-consurf.aln</a><br>Downloaded 121p-consurf.aln to C:\Users\selua\Downloads\121p-consurf(1).aln<br>Alignment identifier is 121p-consurf(1).aln<br>Associated 121p chain A to Input_pdb_SEQRES_A with 0 mismatches<br>Opened 150 sequences from 121p-consurf(1).aln<br><b>color byattribute seq_conservation</b> </blockquote><blockquote class="gmail_quote" style="margin:0px 0px 0px 0.8ex;border-left:1px solid rgb(204,204,204);padding-left:1ex">No known/registered attribute seq_conservation<br></blockquote><div><br></div><img src="cid:ii_kl8xt0u10" alt="image.png" width="512" height="297"><div><br></div><div>Thanks for any help,</div><div>Etowah <br><div> </div></div></div>