<html><head><meta http-equiv="Content-Type" content="text/html; charset=us-ascii"></head><body style="word-wrap: break-word; -webkit-nbsp-mode: space; line-break: after-white-space;" class="">Hi Caroline,<div class=""><br class=""></div><div class=""> Tristan is right, although I think the command may need to be "wait 1" which means draw one frame. (The wait command without arguments waits until ongoing motions complete and I'm not sure if it draws a frame when there are no ongoing motions.) The reason you need to draw a frame is because the volume surface for your new threshold level is not calculated until a frame is drawn. In a script graphics are not normally drawn until the end unless the wait command is used.</div><div class=""><br class=""></div><div class=""> We saw this problem before and I put in a fix where volume surfaces are recomputed when the level changes when running a script. So your script should work without any changes in the current ChimeraX daily build. That fix was made April 7, so I guess you are using a ChimeraX older than that. I'd suggest updating ChimeraX instead of adding "wait 1" to your script.</div><div class=""><br class=""></div><div class=""><span class="Apple-tab-span" style="white-space:pre"> </span>Tom</div><div class=""><br class=""></div><div class=""><div><br class=""><blockquote type="cite" class=""><div class="">On May 7, 2020, at 6:02 AM, Tristan Croll <<a href="mailto:tic20@cam.ac.uk" class="">tic20@cam.ac.uk</a>> wrote:</div><br class="Apple-interchange-newline"><div class=""><meta http-equiv="Content-Type" content="text/html; charset=UTF-8" class=""><div style="font-size: 10pt" class=""><p class="">Try adding the line:</p><p class="">wait</p><p class="">... before those two commands. That will tell ChimeraX to draw a frame and do all the associated processing before moving on.</p>
<div id="signature" class=""> </div><p class=""><br class=""></p><p id="reply-intro" class="">On 2020-05-07 13:52, Caroline Stone wrote:</p>
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<div class=""><p class=""><span style="font-family: Calibri;" class="">Dear All, <br class=""></span></p><p class=""><span style="font-family: Calibri;" class="">As an exercise in scripting, I am trying to write a script to imitate the appearance a published figure (from Toropova et al 2017). I would like to display a semi-transparent map which has been color zoned to match the underlying model in surface view.<br class=""></span></p><p class=""><span style="font-family: Calibri;" class="">ChimeraX does everything in the script apart from the last two commands, in which I color zone the map, and then make it transparent. I don't get any errors in the log to give me a clue what to change. If I omit these last two commands from the script and then make them manually from the command line after the script is finished, it works. Is there something I can change that would allow me to include the final two commands in the script? Any pointers would be much appreciated. <br class=""></span></p><div class=""><span style="font-family: Calibri;" class=""> </span><br class="webkit-block-placeholder"></div><p class=""><span style="font-family: Calibri;" class=""> # ChimeraX script to imitate dynein 2 tail Toropova et al 2017 figure style<br class=""> # Adds custom colours to the ChimeraX session<br class=""><br class=""> # Open model and set camera view and model rotation<br class=""> open 6RLB <br class=""> windowsize 775 775<br class=""> view matrix camera -0.30533,0.56038,0.76991,623.53,0.82837,-0.24247,0.50499,467.83,0.46967,0.79195,-0.39016,-7.3434 models #1,1,0,0,0,0,1,0,0,0,0,1,0<br class=""><br class=""> # Work with model representation<br class=""> surface #1<br class=""> set bgColor white<br class=""> lighting soft<br class=""> graphics silhouettes true<br class=""><br class=""> # Define custom colours<br class=""> color name midblue #6b80b5<br class=""> color name midgreen #a0c497<br class=""> color name midyellow #f9ef87<br class=""> color name midtomato #f97054<br class=""> color name midmagenta #a4449b<br class=""><br class=""> # Recolour model using custom colours defined above<br class=""> select #1/A; color sel midblue<br class=""> select #1/B; color sel skyblue<br class=""> select #1/C; color sel midtomato<br class=""> select #1/D; color sel midmagenta<br class=""> select #1/E/F; color sel midgreen<br class=""> select #1/G/H; color sel lightpink<br class=""> select #1/I-N; color sel midyellow<br class=""> select clear<br class=""><br class=""> # Open map and modify color and transparency<br class=""> open emdb:4918<br class=""> volume #2 step 1<br class=""> volume #2 level 0.0172<br class=""> surface dust #2 size 10<br class=""> color zone #2 near #1 distance 15<br class=""> volume #2 transparency 0.75</span></p><div class=""><span style="font-family: Calibri;" class=""> </span><br class="webkit-block-placeholder"></div><p class=""><span style="font-family: Calibri;" class="">Best wishes, <br class="">Caroline<br class=""></span></p>
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