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<p style="margin: 0cm 0cm 8pt; line-height: 107%; font-size: 11pt; font-family: Calibri, sans-serif;margin-bottom:0cm; margin-bottom:.0001pt; line-height:
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<span lang="EN-US" style="font-size:12.0pt;
mso-ascii-font-family:Calibri;mso-fareast-font-family:"Times New Roman";
mso-hansi-font-family:Calibri;mso-bidi-font-family:Calibri;color:black;
mso-ansi-language:EN-US;mso-fareast-language:ES">Hi, I am new PhD student
using ChimeraX and I am very interested in learning how to work with this program.</span></p>
<p style="margin: 0cm 0cm 8pt; line-height: 107%; font-size: 11pt; font-family: Calibri, sans-serif;margin-bottom:0cm; margin-bottom:.0001pt; line-height:
normal">
<span lang="EN-US" style="font-size:12.0pt;
mso-ascii-font-family:Calibri;mso-fareast-font-family:"Times New Roman";
mso-hansi-font-family:Calibri;mso-bidi-font-family:Calibri;color:black;
mso-ansi-language:EN-US;mso-fareast-language:ES">I am trying to introduce
some mutations in my protein in some specific amino acids that I have selected. When I introduce an Alanine in my position of interest (using dunbrack) I have no issues, but when I try any other amino acids (<span style="border:none windowtext 1.0pt;
mso-border-alt:none windowtext 0cm;padding:0cm">as
Phe or Thy) the program has no problem giving me the prediction for the rotamers, but I have some issues. After select some of the most probable rotamers, all the amino acids of my protein change to that kind of amino acid (not just the specific one that I
have selected).</span></span></p>
<p style="margin: 0cm 0cm 8pt; line-height: 107%; font-size: 11pt; font-family: Calibri, sans-serif;margin-bottom:0cm; margin-bottom:.0001pt; line-height:
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<span lang="EN-US" style="font-size:12.0pt;
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<br style="mso-special-character:line-break">
</span><span lang="EN-US" style="font-size:12.0pt;mso-ascii-font-family:
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mso-bidi-font-family:Calibri;color:black;mso-ansi-language:EN-US;mso-fareast-language:
ES"></span></p>
<p style="margin: 0cm 0cm 8pt; line-height: 107%; font-size: 11pt; font-family: Calibri, sans-serif;margin-bottom:0cm; margin-bottom:.0001pt; line-height:
normal">
<span lang="EN-US" style="font-size:12.0pt;
mso-ascii-font-family:Calibri;mso-fareast-font-family:"Times New Roman";
mso-hansi-font-family:Calibri;mso-bidi-font-family:Calibri;color:black;
mso-ansi-language:EN-US;mso-fareast-language:ES">Another problem is
that I have is that when I select a specific atom of an amino acid, the program also select similar atoms but in all the others amino acids of my protein.</span></p>
<p style="margin: 0cm 0cm 8pt; line-height: 107%; font-size: 11pt; font-family: Calibri, sans-serif;margin-bottom:0cm; margin-bottom:.0001pt; line-height:
normal">
<span lang="EN-US" style="font-size:12.0pt;
mso-ascii-font-family:Calibri;mso-fareast-font-family:"Times New Roman";
mso-hansi-font-family:Calibri;mso-bidi-font-family:Calibri;color:black;
mso-ansi-language:EN-US;mso-fareast-language:ES">I really hope that
someone can help me with these issues.</span></p>
<p style="margin: 0cm 0cm 8pt; line-height: 107%; font-size: 11pt; font-family: Calibri, sans-serif;margin-bottom:0cm; margin-bottom:.0001pt; line-height:
normal">
<span lang="EN-US" style="font-size:12.0pt;
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mso-hansi-font-family:Calibri;mso-bidi-font-family:Calibri;color:black;
mso-ansi-language:EN-US;mso-fareast-language:ES">Thank You very much</span></p>
<p style="margin: 0cm 0cm 8pt; line-height: 107%; font-size: 11pt; font-family: Calibri, sans-serif;margin-bottom:0cm; margin-bottom:.0001pt; line-height:
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<span lang="EN-US" style="font-size:12.0pt;
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mso-ansi-language:EN-US;mso-fareast-language:ES"><o:p> </o:p></span></p>
<p style="margin: 0cm 0cm 8pt; line-height: 107%; font-size: 11pt; font-family: Calibri, sans-serif;margin-bottom:0cm; margin-bottom:.0001pt; line-height:
normal; background:white">
<span lang="EN-US" style="font-size:10.0pt;font-family:"Tahoma",sans-serif;mso-fareast-font-family:
"Times New Roman";color:#323130;border:none windowtext 1.0pt;mso-border-alt:
none windowtext 0cm;padding:0cm;mso-ansi-language:EN-US;mso-fareast-language:
ES">Alejandro
Llorente.</span><span lang="EN-US" style="font-size:10.0pt;
font-family:"Tahoma",sans-serif;mso-fareast-font-family:"Times New Roman";
color:#323130;mso-ansi-language:EN-US;mso-fareast-language:ES"></span></p>
<p style="margin: 0cm 0cm 8pt; line-height: 107%; font-size: 11pt; font-family: Calibri, sans-serif;margin-bottom:0cm; margin-bottom:.0001pt; line-height:
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<span lang="EN-US" style="font-size:10.0pt;font-family:"Tahoma",sans-serif;mso-fareast-font-family:
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ES"><br>
Lab of Dr. Nancy Carrasco.<br>
Department of Molecular Physiology & Biophysics, Vanderbilt University.<br style="mso-special-character:line-break">
<br style="mso-special-character:line-break">
</span><span lang="EN-US" style="font-size:10.0pt;font-family:"Tahoma",sans-serif;
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Garland Ave Nashville, TN 37232-0615, room 711.</span><span lang="EN-US" style="font-size:10.0pt;font-family:"Tahoma",sans-serif;mso-fareast-font-family:
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phone: +34 626586236</span><span style="font-size:10.0pt;
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