<html><head><meta http-equiv="Content-Type" content="text/html; charset=utf-8"></head><body style="word-wrap: break-word; -webkit-nbsp-mode: space; line-break: after-white-space;" class="">One other note — if your graphics window on screen is not square and you save the image as square some padding is going to be done which you probably don’t want. So save the image specifying "width 4000” and don’t specify the height — then the height will be chosen to give the same aspect ratio as on screen. Or you can use the "windowsize 600 600” command to make sure your window is square.<div class=""><br class=""></div><div class=""><span class="Apple-tab-span" style="white-space:pre"> </span>Tom<br class=""><div><br class=""><blockquote type="cite" class=""><div class="">On Jul 31, 2019, at 4:59 PM, Tom Goddard <<a href="mailto:goddard@sonic.net" class="">goddard@sonic.net</a>> wrote:</div><br class="Apple-interchange-newline"><div class=""><meta http-equiv="Content-Type" content="text/html; charset=utf-8" class=""><div style="word-wrap: break-word; -webkit-nbsp-mode: space; line-break: after-white-space;" class="">Hi Yazan,<div class=""><br class=""></div><div class=""> The zoom command of course gives the pixels size shown on the screen (what else could it give you). I’m guessing our graphics window on the screen was not 4000 by 4000 pixels, you can find out what it really is (or you can set it) with the windowsize command. So if your graphics window size on screen is 1000 by 1000 and your image is 4000 by 4000 and zoom reports pixel size 0.4 Angstroms then the image will have pixel size 0.1 Angstroms.</div><div class=""><br class=""></div><div class=""><span class="Apple-tab-span" style="white-space:pre"> </span>Tom<br class=""><div class=""><br class=""><blockquote type="cite" class=""><div class="">On Jul 31, 2019, at 4:43 PM, Yazan Abbas <<a href="mailto:yazan.abbas@gmail.com" class="">yazan.abbas@gmail.com</a>> wrote:</div><br class="Apple-interchange-newline"><div class=""><div dir="ltr" class="">Hi,<br class=""><div class=""><br class=""></div><div class="">I am trying to create a scale bar using the following method:</div><div class=""><ol class=""><li class="">camera is set to ortho</li><li class="">save image image.png width 4000 height 4000 transparentBackground true</li><li class="">check pixel size in chimeraX using the zoom command (which shows Pixel size at center of rotation is 0.178)<br class=""></li><li class="">in imageJ, set the scale to be 0.178Å/pixel which turns into 5.618 pixels/Å</li><li class="">Show scale bar of desired in length in ImageJ</li></ol><div class="">However, the resulting scale bar looks unusually small given the dimensions of my particle. Have I misunderstood something about the pixelSize settings? The user guide doesn't say anything about pixelSize when one of or both of width and height are specified in the same image command, so I assumed the pixel size of the saved image would be the same as in the graphics window.</div></div><div class=""><br class=""></div><div class="">Thanks, </div><div class=""><br class=""></div><div class="">Yazan</div></div>
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