<div dir="ltr">I'd add that there is an important difference between Gaussian filtering and band-pass filtering: Gaussian filtering blurs (sharpens) everything by a specified amount, whereas low-pass (high-pass) filtering imposes a minimum amount of blurriness (sharpness). This often makes band-pass filtering the right choice for images with heterogenous resolution, because you often want to blur the noisiest, sharpest parts without blurring the smoothest, blurriest parts. Cryo-EM maps often fall in this category, and thus represent a common use case.</div><br><div class="gmail_quote"><div dir="ltr">On Wed, May 10, 2017 at 2:54 AM Tom Goddard <<a href="mailto:goddard@sonic.net">goddard@sonic.net</a>> wrote:<br></div><blockquote class="gmail_quote" style="margin:0 0 0 .8ex;border-left:1px #ccc solid;padding-left:1ex">This is just like ChimeraX Gaussian filtering which does a convolution with a Gaussian in Fourier space. That is relatively fast — a few seconds for a 256**3 map. You can already do sharpening with the “invert” option to the gaussian filtering command (“vol gaussian #1 1.5 invert true”). Gaussian filtering is one form of low pass filtering — others could be added. I think it would be nice to allow a very fast (10 updates per second) test filtering on a small region using a mouse drag to figure out the desired parameters. But I never spent to the time to figure out a user interface (what small region, how do you undo, how do you then apply to full map, …).<br>
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Tom<br>
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> On May 6, 2017, at 10:27 AM, Oliver Clarke <<a href="mailto:olibclarke@gmail.com" target="_blank">olibclarke@gmail.com</a>> wrote:<br>
><br>
> Hi,<br>
><br>
> Has any consideration been given to adding the capacity for fourier-space map sharpening/low pass filtering to ChimeraX? I’m not sure whether or not it is feasible speed wise, but if it is it would be a very useful addition for EM, allowing one to easily try different sharpening B-factors/low pass cutoffs and save the resulting map(s).<br>
><br>
> Cheers<br>
> Oli<br>
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