<div dir="ltr"><div>Dear <span>Chimera</span> company<span style="color:rgb(14,16,26)"><br></span></div><div><span style="color:rgb(14,16,26)"><br></span></div><div>Thank you for always answering my questions so carefully.<span style="color:rgb(14,16,26)"><br></span></div><div><span style="color:rgb(14,16,26)">Thank you for allowing me to use your software. I am Japanese, so I hope you will understand that there are some inappropriate expressions. I would like to ask you one question.</span><br></div><div><span style="color:rgb(14,16,26)"><br></span></div><div>I entered the data as per the manual. However, the hydrogen bond did not show up. What should I do?<span style="color:rgb(14,16,26)"><br></span></div><div><br></div><div><font color="#0e101a">The figure of Parameters</font></div><div><span style="color:rgb(14,16,26)"><br></span></div><div><img src="cid:ii_ky45vsiq1" alt="image.png" width="309" height="412"><br></div><div><span style="color:rgb(14,16,26)"><br></span></div><div><span style="color:rgb(14,16,26)">The result of showing </span></div><div><img src="cid:ii_ky44oa9v0" alt="osh4,ID 223-230 .png" width="391" height="331" style="margin-right: 0px;"><br></div><div><br></div><div>Details on how to do it<br></div><div><br></div><div><p class="MsoNormal" style="margin:0mm;text-align:justify;font-size:10.5pt;font-family:游明朝,serif"><strong><span lang="EN-US" style="font-family:Arial,sans-serif;color:black;background-image:initial;background-position:initial;background-size:initial;background-repeat:initial;background-origin:initial;background-clip:initial">Display interactions between ligand and protein.</span></strong><strong><span lang="EN-US" style="font-family:Arial,sans-serif;color:black;background-image:initial;background-position:initial;background-size:initial;background-repeat:initial;background-origin:initial;background-clip:initial;font-weight:normal"></span></strong></p>
<p class="MsoNormal" style="margin:0mm;text-align:justify;font-size:10.5pt;font-family:游明朝,serif"><span lang="EN-US">1.Favorites</span>→<span lang="EN-US">Model
Panel</span>→<span lang="EN-US" style="font-family:Arial,sans-serif;background-image:initial;background-position:initial;background-size:initial;background-repeat:initial;background-origin:initial;background-clip:initial">group/ungroup( Go down at close.)</span><span lang="EN-US"></span></p>
<img src="cid:ii_ky9htqjc2" alt="image.png" width="412" height="296"><br>
<p class="MsoNormal" style="margin:0mm;text-align:justify;font-size:10.5pt;font-family:游明朝,serif"><span lang="EN-US">2.Favorite</span>→<span lang="EN-US">Command
line: </span><span lang="EN-US" style="font-family:Arial,sans-serif;background-image:initial;background-position:initial;background-size:initial;background-repeat:initial;background-origin:initial;background-clip:initial">combine #0.1,0.2</span></p>
<p class="MsoNormal" style="margin:0mm;text-align:justify;font-size:10.5pt;font-family:游明朝,serif"><span style="background-image:initial;background-position:initial;background-size:initial;background-repeat:initial;background-origin:initial;background-clip:initial">→</span><span lang="EN-US" style="font-family:Arial,sans-serif;background-image:initial;background-position:initial;background-size:initial;background-repeat:initial;background-origin:initial;background-clip:initial">select
combination at model Panel </span></p>
<img src="cid:ii_ky9htxsm3" alt="image.png" width="412" height="95"><br>
<p class="MsoNormal" style="margin:0mm;text-align:justify;font-size:10.5pt;font-family:游明朝,serif"><span style="font-size:10.5pt"> </span><br></p>
<p class="MsoNormal" style="margin:0mm;text-align:justify;font-size:10.5pt;font-family:游明朝,serif"><span lang="EN-US">3.rename </span><span lang="EN-US"></span></p>
<p class="MsoNormal" style="margin:0mm;text-align:justify;font-size:10.5pt;font-family:游明朝,serif"><span lang="EN-US">4.Select </span>→<span lang="EN-US">Residue</span>→<span lang="EN-US"> LIG</span> <span lang="EN-US">or command: select :LIG</span></p>
<p class="MsoNormal" style="margin:0mm;text-align:justify;font-size:10.5pt;font-family:游明朝,serif"><span lang="EN-US">5.Tool</span>→<span lang="EN-US">Surface/Binding
Analysis</span>→ <span lang="EN-US">Find H Bond</span></p>
<img src="cid:ii_ky9huzxb4" alt="image.png" width="412" height="245"><br>
<p class="MsoNormal" style="margin:0mm;text-align:justify;font-size:10.5pt;font-family:游明朝,serif"><span lang="EN-US">Check</span><span lang="EN-US" style="font-family:Arial,sans-serif;background-image:initial;background-position:initial;background-size:initial;background-repeat:initial;background-origin:initial;background-clip:initial"> three
points</span></p>
<p class="MsoNormal" style="margin:0mm;text-align:justify;font-size:10.5pt;font-family:游明朝,serif"><span lang="EN-US" style="font-family:Arial,sans-serif;background-image:initial;background-position:initial;background-size:initial;background-repeat:initial;background-origin:initial;background-clip:initial">1."Only find H-bonds..." e.g.
"with at least one end selected".</span></p>
<p class="MsoNormal" style="margin:0mm;text-align:justify;font-size:10.5pt;font-family:游明朝,serif"><span lang="EN-US" style="font-family:Arial,sans-serif;background-image:initial;background-position:initial;background-size:initial;background-repeat:initial;background-origin:initial;background-clip:initial">2. "If endpoint atom hidden, shown
endpoint residue" or else it will not show all of the H-bonds it finds</span><span lang="EN-US"></span></p>
<p class="MsoNormal" style="margin:0mm;text-align:justify;font-size:10.5pt;font-family:游明朝,serif"><span lang="EN-US">3.Write information to reply log </span></p>
<p class="MsoNormal" style="margin:0mm;text-align:justify;font-size:10.5pt;font-family:游明朝,serif"><span lang="EN-US">Apply</span>→<span lang="EN-US">OK</span></p></div><br clear="all"><div><div dir="ltr" data-smartmail="gmail_signature"><div dir="ltr"><div><span style="color:rgb(34,34,34)">------------------------------</span><span style="color:rgb(34,34,34)">------------------------------</span><span style="color:rgb(34,34,34)">---------------</span><br></div>松井 健治<div> 東京農工大学大学院 工学府産業技術専攻 修士 </div><div>〒184-8588 東京都小金井市中町2-24-16</div><div> Mail: <a href="mailto:s214903z@st.go.tuat.ac.jp" target="_blank">s214903z@st.go.tuat.ac.jp</a><br></div><div><br></div><div><span style="color:rgb(34,34,34)">Kenji Matsui</span><br style="color:rgb(34,34,34)"><span style="color:rgb(34,34,34)"> Graduate School of Tokyo University of Agriculture and Technology</span><br style="color:rgb(34,34,34)"><span style="color:rgb(34,34,34)">2-24-16, Nakamachi, Koganei-shi, Tokyo 184-8588, Japan</span><br style="color:rgb(34,34,34)"><div style="color:rgb(34,34,34)"> Mail: <a href="mailto:s214903z@st.go.tuat.ac.jp" style="color:rgb(17,85,204)" target="_blank">s214903z@st.go.tuat.ac.jp</a></div></div></div></div></div></div>