<html><head></head><body><div class="yahoo-style-wrap" style="font-family:times new roman, new york, times, serif;font-size:16px;"><div dir="ltr" data-setdir="false">Hello chimera-users,</div><div dir="ltr" data-setdir="false"><br></div><div dir="ltr" data-setdir="false">I would like to introduce a-helices into my protein structure ab initio.</div><div dir="ltr" data-setdir="false"><br></div><div dir="ltr" data-setdir="false">Is it possible to do this with chimera?</div><div dir="ltr" data-setdir="false"><br></div><div dir="ltr" data-setdir="false">Another option that I have is to take a-helical pieces of sequence from a different model</div><div dir="ltr" data-setdir="false"><br></div><div dir="ltr" data-setdir="false">and introduce these into the desired model.</div><div dir="ltr" data-setdir="false"><br></div><div dir="ltr" data-setdir="false">Would that be more feasible?</div><div dir="ltr" data-setdir="false"><br></div><div dir="ltr" data-setdir="false">There is a chance I also need to introduce loops and turns ab initio to best localize the</div><div dir="ltr" data-setdir="false"><br></div><div dir="ltr" data-setdir="false">introduced a-helices within my protein structure.</div><div dir="ltr" data-setdir="false"><br></div><div dir="ltr" data-setdir="false">Can you please indicate how can a chimera user build all this ab initio?</div><div dir="ltr" data-setdir="false"><br></div><div dir="ltr" data-setdir="false"><br></div><div dir="ltr" data-setdir="false">Thank you in advance for your time and help,</div><div dir="ltr" data-setdir="false"><br></div><div dir="ltr" data-setdir="false">Chara Spanou</div><div dir="ltr" data-setdir="false">PhD student</div><div dir="ltr" data-setdir="false">University of Cologne</div><div dir="ltr" data-setdir="false"><br></div></div></body></html>