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<p>Hi There,<br>
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<p>I hope you are doing very well. My name is Roksana Azad, I am a Ph.D. Student at the CUNY ASRC.<br>
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<p>I have been working on building a full length model of my protein into a cryoEM map, I have models for individual domain but not for the full lenght structure. I am trying to join the domain models using chimera "join models" function, but unfortunately
it's not working for me. I tried everything I could find online about the issue, I used the
<em>addh</em> command and <em>kssdssp </em>commands and also tried joining them using other bond instead of C-N, however my button for "apply" always grey and I can't click on it (please see the screenshot attached).<br>
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<p>I also tried this command "#0:127.A@C #1:1.A@N" according to my model # and atom, but its not selecting anything for me. <span style="font-family: Calibri, Arial, Helvetica, sans-serif; font-size: 16px; background-color: rgb(255, 255, 255);">I tried "combine"
it also didn't work.</span><br>
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<p>Please let me know if there is something else I am missing or what else I can do to create this bond or connection between two models.<br>
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<p>Thank you so much for your time and help in advance – very much appreciated.<br>
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<p>Sincerely,<br>
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<p>Roksana<br>
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<font face="Arial,Helvetica,sans-serif" size="4" style="font-size:16px; background-color:rgb(255,255,255)"><b><a href="https://asrc.gc.cuny.edu/people/roksana-azad/" id="NoLP">Roksana Azad</a></b></font><br style="font-size:16px; background-color:rgb(255,255,255)">
<font face="Arial,Helvetica,sans-serif" style="font-size:16px; background-color:rgb(255,255,255)">Ph.D. Candidate in Biochemistry & Biophysics</font><br style="font-size:16px; background-color:rgb(255,255,255)">
<font face="Arial,Helvetica,sans-serif" style="font-size:16px; background-color:rgb(255,255,255)">Advisor: </font><font face="Arial,Helvetica,sans-serif" style="font-size:16px; background-color:rgb(255,255,255)"><a href="https://kglab.ws.gc.cuny.edu/" id="NoLP">Kevin
H. Gardner</a></font><br style="font-size:16px; background-color:rgb(255,255,255)">
<font face="Arial,Helvetica,sans-serif" style="font-size:16px; background-color:rgb(255,255,255)">Structural Biology Initiative; CUNY Advanced Science Research Center</font><br style="font-size:16px; background-color:rgb(255,255,255)">
<font face="Arial,Helvetica,sans-serif" style="font-size:16px; background-color:rgb(255,255,255)">85 St Nicholas Terrace • New York • NY • 10031</font><br style="font-size:16px; background-color:rgb(255,255,255)">
<font face="Arial,Helvetica,sans-serif" style="font-size:16px; background-color:rgb(255,255,255)">Phone: 212-413-3233</font><br style="font-size:16px; background-color:rgb(255,255,255)">
<font face="Arial,Helvetica,sans-serif" style="font-size:16px; background-color:rgb(255,255,255)">Email: razad@gc.cuny.edu</font></div>
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