<html><head><meta http-equiv="Content-Type" content="text/html; charset=utf-8"></head><body style="word-wrap: break-word; -webkit-nbsp-mode: space; line-break: after-white-space;" class=""><div class="">Dear Experts,</div><div class=""><br class=""></div><div class="">This is regarding adding an ALA residue in the C-terminal of a specific protein domain, with CHIMERA.</div><div class=""><br class=""></div><div class="">I have a protein-complex with multiple domains and chains.</div><div class="">I want to add an ALA residue (101-th position) in the chain D, after the 100th residue which is PRO.</div><div class=""><br class=""></div><div class="">I followed the tutorial and have given the following command in command line:</div><div class=""><br class=""></div><div class=""><b class="">addaa ala,101 :100.d</b></div><div class=""><br class=""></div><div class="">The following outcome was: Couldn’t find ‘O’ backbone atom of PRO 100.D.</div><div class=""><br class=""></div><div class="">Request you to kindly let me know what the possible solution could be, so that I add an ALA residue in the C-terminal of Chain D.</div><div class=""><br class=""></div><div class="">Thank you in advance,</div><div class="">Regards,</div><div class="">Prathit Chatterjee</div><div class=""><br class=""></div><div class=""><br class=""></div><div class=""><br class=""></div></body></html>