<div dir="auto"><div>Dear Elaine,</div><div dir="auto"><br></div><div dir="auto">Thank you for your reply. When I am trying to fit model using reference pdb. I am not getting appropriate response. Because my reference pdb has only 11 chains while my protein has more than this. Kindly suggest some person who can help me with this. I will be heartily thankful to you</div><div dir="auto"><br></div><div dir="auto"><br></div><div dir="auto">Regards</div><div dir="auto">Neha Goel</div><div dir="auto"><br><div class="gmail_quote" dir="auto"><div dir="ltr" class="gmail_attr">On Mon, 10 May 2021, 20:51 Elaine Meng, <<a href="mailto:meng@cgl.ucsf.edu">meng@cgl.ucsf.edu</a>> wrote:<br></div><blockquote class="gmail_quote" style="margin:0 0 0 .8ex;border-left:1px #ccc solid;padding-left:1ex">Hi Neha,<br>
An MRC file is a density map format. You cannot save it as an atomic model, because it does not contain atomic coordinates. <br>
<br>
There is no tool in Chimera to create atomic structure instantly from a map. Researchers use various programs to build atomic structures into a map or to build an atomic model separately and then fit it into a map, but it is generally an interactive process that takes many steps. I am not an expert on this process, but you could read hundreds of research papers "Methods" sections to see descriptions of the ways in which it is done.<br>
<br>
Regards,<br>
Elaine<br>
-----<br>
Elaine C. Meng, Ph.D. <br>
UCSF Chimera(X) team<br>
Department of Pharmaceutical Chemistry<br>
University of California, San Francisco<br>
<br>
> On May 10, 2021, at 10:24 AM, Neha Goel <<a href="mailto:nehagoel24march@gmail.com" target="_blank" rel="noreferrer">nehagoel24march@gmail.com</a>> wrote:<br>
> <br>
> Dear Chimera,<br>
> I have a .mrc file. I reopened the file in chimera and would like to save it in pdb. I am unable to save it. It needed the molecular model pdb. Kindly guide me the way to save it.<br>
> Regards<br>
> Neha Goel<br>
<br>
</blockquote></div></div></div>