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Hi Elaine</div>
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I've been using the biological assembly of 3R2P and I'm thinking that the reason why it's not working is because both chains are labeled A. I'm just following a paper where they're building a monomeric structure using the residue fragments but in their case
the biological assembly of 3R2P is labeled as chain A and chain B. </div>
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<div id="divRplyFwdMsg" dir="ltr"><font face="Calibri, sans-serif" style="font-size:11pt" color="#000000"><b>From:</b> Elaine Meng <meng@cgl.ucsf.edu><br>
<b>Sent:</b> Monday, February 8, 2021 1:01 PM<br>
<b>To:</b> Moradkhan, Tiglath A <tmoradkhan@csus.edu><br>
<b>Cc:</b> chimera-users@cgl.ucsf.edu <chimera-users@cgl.ucsf.edu><br>
<b>Subject:</b> Re: [Chimera-users] Creating new models from existing crystal structures</font>
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<div class="PlainText">Hi Tiglath,<br>
If you just opened 3r2p one time, you only have one model. Join works on bonding two separate models. Also it contains only chain A (there is no chain B in this structure) and residue 130 in chain A is already bonded to residue 131 in chain A. So I do not
understand what you already did to modify the structure(s) before joining, or what you are trying to do now, sorry.<br>
<br>
You could send a session file if you want.<br>
Elaine<br>
-----<br>
Elaine C. Meng, Ph.D. <br>
UCSF Chimera(X) team<br>
Department of Pharmaceutical Chemistry<br>
University of California, San Francisco<br>
<br>
> On Feb 8, 2021, at 12:35 PM, Moradkhan, Tiglath A <tmoradkhan@csus.edu> wrote:<br>
> <br>
> Hi<br>
> <br>
> I'm trying to join two models together using the Build Structure tool but I'm having problems. Here is my process.<br>
> <br>
> • Using the 3R2P crystal structure I select residues 3-130 from chain A and 131-182 from chain B<br>
> • Then using the following command in Chimera I select the N atom of residue 130 that will form a bond with the C atom of residue 131: sel #0:130@N#1:131@C (#0 is the name of the 3-130 segment and #1 is the name of the 131-182 segment)<br>
> • Then using the Build Structure tool I select Form C-N peptide bond, but the Apply button doesn't activate.<br>
> I don't understand what the issue is. Is the command that I've written wrong?<br>
> <br>
> I would be very glad for your help. I can provide the pdb files of the residue segments if necessary.<br>
> Thanks<br>
> <br>
> Tiglath<br>
<br>
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