<html><body style="word-wrap: break-word; -webkit-nbsp-mode: space; line-break: after-white-space;">Hello Angus and Michael,<div class="">It may be a function of the map display style. If I open a map from EMDB, e.g. commands:<div class=""><br class=""></div><div class="">open emdbID:1024</div><div class="">stereo anaglyph</div><div class=""><br class=""></div><div class="">I can clearly see the 3D effect when wearing red-cyan glasses (and red and cyan fringes with the glasses taken off) if I am showing surface or mesh isosurfaces for the map, but not if showing the diffuse cloud of the "solid" style. I attach an image of the map in surface style.</div><div class=""><br class=""></div><div class="">I don't know if you can expect to see a 3D effect with the transparent "solid" style even with anaglyph stereo working correctly. I couldn't perceive it with that style.</div><div class=""><br class=""></div><div class="">Tested Chimera 1.14 on Mac, and I believe that the map from EMDB is 32-bit floating point like your data.</div><div class=""><br class=""></div><div class="">I hope this helps,</div><div class="">Elaine<br class=""><div class="">-----<br class="">Elaine C. Meng, Ph.D. <br class="">UCSF Chimera(X) team<br class="">Department of Pharmaceutical Chemistry<br class="">University of California, San Francisco<br class=""><br class=""><br class=""><img apple-inline="yes" id="76DA29AD-D12E-4574-BE2E-C535D2777F3D" src="cid:EFA38621-17EC-4C34-8612-2AE9F54C88AC@gateway.sonic.net" class=""></div><br class=""><blockquote type="cite" class="">On Sep 22, 2020, at 10:03 AM, Greg Couch <<a href="mailto:gregc@cgl.ucsf.edu" class="">gregc@cgl.ucsf.edu</a>> wrote:<br class=""><br class="">Yes, the red and gray is normal, but you're seeing the combined channels. You need the glasses to separate the channels. Try viewing the image with the glasses that come with a 3D comic book.<br class=""><br class=""> -- Greg<br class=""><br class="">On 9/21/2020 11:43 PM, Michael Elbaum wrote:<br class=""><blockquote type="cite" cite="mid:DM6PR04MB5065A2A23F29449F0D8B980EFE3B0@DM6PR04MB5065.namprd04.prod.outlook.com" style="font-family: Helvetica; font-size: 13px; font-style: normal; font-variant-caps: normal; font-weight: normal; letter-spacing: normal; orphans: auto; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: auto; word-spacing: 0px; -webkit-text-size-adjust: auto; -webkit-text-stroke-width: 0px; text-decoration: none;" class="">Red-cyan, sorry. No, I didn't look for stereo at all, just to reproduce the condition posted that one channel appears in red and the other in gray. Is that normal? <br class="">Michael<br class="">From: Greg Couch <<a href="mailto:gregc@cgl.ucsf.edu" class="">gregc@cgl.ucsf.edu</a>><br class="">Sent: Tuesday, September 22, 2020 4:57:50 AM<br class="">To: Michael Elbaum <<a href="mailto:michael.elbaum@weizmann.ac.il" class="">michael.elbaum@weizmann.ac.il</a>><br class="">Subject: Re: [Chimera-users] Stereo Red Cyan<br class=""> <br class="">Are you wearing red-cyan glasses to see the stereo? It doesn't work without them.<br class=""><br class=""> -- Greg<br class=""><br class="">On 9/21/2020 1:08 PM, Michael Elbaum wrote:<br class=""><blockquote type="cite" class="">I'm confirming the problem with a different dataset (optical deconvolution in tif format). Red-green shows red and gray, green-magenta shows green and gray. This is with version 1.14 on linux.<br class="">regards,<br class="">Michael<br class=""><br class=""><br class="">From: Chimera-users <<a href="mailto:chimera-users-bounces@cgl.ucsf.edu" class="">chimera-users-bounces@cgl.ucsf.edu</a>> on behalf of Angus McDonald <<a href="mailto:amcdonald@boisestate.edu" class="">amcdonald@boisestate.edu</a>><br class="">Sent: Monday, September 21, 2020 9:46:24 PM<br class="">To: <a href="mailto:chimera-users@cgl.ucsf.edu" class="">chimera-users@cgl.ucsf.edu</a> BB<br class="">Subject: [Chimera-users] Stereo Red Cyan<br class=""> <br class="">I am looking at some cryo em data. When I try to view a a 3D stack of em dat with the Red-Cyan stereo setting I seem to only get Red and grayscale. The data is in 32-bit floating point and is in MRC2014 header format. I cannot figure out what I am doing wrong. Is anything obvious? <br class=""><br class="">Thanks for your time.<br class="">-- <br class="">Angus McDonald<br class="">amcdonald@boisestate.edu<br class=""> <br class=""></blockquote></blockquote></blockquote><br class=""></div></div></body></html>