<html><body style="word-wrap: break-word; -webkit-nbsp-mode: space; line-break: after-white-space;">Hi Zaher,<div class="">First use the Multalign Viewer (sequence window) Headers menu to show the RMSD histogram(s) that you want: CA, backbone, and/or full. Then use that same Headers menu "Save..." option to save to file with RMSD vs. alignment position.</div><div class=""><br class=""></div><div class=""><<a href="http://www.rbvi.ucsf.edu/chimera/docs/ContributedSoftware/multalignviewer/framemav.html" class="">http://www.rbvi.ucsf.edu/chimera/docs/ContributedSoftware/multalignviewer/framemav.html</a>><br class=""><http://www.rbvi.ucsf.edu/chimera/docs/ContributedSoftware/multalignviewer/multalignviewer.html#headers></div><div class=""><br class=""></div><div class="">This example image shows all three RMSD histograms:</div><div class=""><img apple-inline="yes" id="CC722A64-EAA2-4CF1-8B97-D9AEAEC8E1D1" width="320" height="189" src="cid:9DFEC75C-B995-4300-9C1E-7C978A87D02E@cgl.ucsf.edu" class=""></div><div class=""><br class=""></div><div class="">... or if you want the list to be RMSD vs. structure residue numbering instead of alignment numbering, start the "Render by Attribute" tool (in menu under Tools... Structure Analysis) and use that tool's File menu to save the corresponding residue attribute(s) named "mavRMSD[...]". This is possible because sequence alignment header values are automatically assigned as attributes of the associated residues.<br class=""><div class=""><br class="webkit-block-placeholder"></div><div class=""><<a href="http://www.rbvi.ucsf.edu/chimera/docs/ContributedSoftware/render/render.html#saving" class="">http://www.rbvi.ucsf.edu/chimera/docs/ContributedSoftware/render/render.html#saving</a>></div><div class=""><br class="webkit-block-placeholder"></div><div class="">In ChimeraX, you can only show one RMSD header at a time but you can choose whether it is ca, backbone, or full. The ChimeraX Sequence Viewer has its own menu including Headers to control this and also saving the header to file, but instead of on the top bar it is a context menu shown with right-click or ctrl-click, depending on your type of computer. The attribute is also assigned but ChimeraX does not yet have saving attributes to file. </div><div class=""><br class="webkit-block-placeholder"></div><div class="">I hope this helps,</div><div class="">Elaine</div><br class=""><blockquote type="cite" class="">On Sep 18, 2020, at 12:38 PM, <a href="mailto:arrafzaher@campus.technion.ac.il" class="">arrafzaher@campus.technion.ac.il</a> wrote:<br class=""><br class="">Thank you Elaine,<br class="">No worries.<br class="">One Last Question (Hopefully),<br class="">Is there a way to produce a datasheet of RMSD for each position aligned from the 3 structures?<br class="">Zaher<br class=""><br class=""></blockquote><br class=""></div></body></html>