<html><body style="word-wrap: break-word; -webkit-nbsp-mode: space; line-break: after-white-space;">Hello,<div class="">I changed the "Subject" line to something useful.</div><div class=""><br class=""></div><div class="">Chimera does not automatically renumber the protein residue sequence.</div><div class=""><br class=""></div><div class="">I tried opening 5trf directly from the PDB and the chains start numbering at 10, for example chain A shown with commands:</div><div class=""><br class=""></div><div class="">open 5trf</div><div class="">~display</div><div class="">~ribb ~:.a</div><div class="">rl :1-20</div><div class=""><br class=""></div><div class=""><img apple-inline="yes" id="7F4C1EFE-1702-4CE6-8FD0-0634B9EBC9A3" src="cid:F35D3777-B756-4089-B099-4EBE7ED0D912@gateway.sonic.net" class=""></div><div class=""><br class=""></div><div class="">I agree that using "File... Save Mol2" renumbers the residues in the Mol2 file. </div><div class=""><br class=""></div><div class="">However, if you use "File... Save PDB" it does not renumber, so unless you have some other reason for using Mol2, save as PDB instead.</div><div class=""><br class=""></div><div class="">I hope this helps,</div><div class="">Elaine</div><div class=""><div class="">-----<br class="">Elaine C. Meng, Ph.D. <br class="">UCSF Chimera(X) team<br class="">Department of Pharmaceutical Chemistry<br class="">University of California, San Francisco<br class=""><br class=""></div><br class=""><blockquote type="cite" class="">On Aug 21, 2020, at 12:23 AM, Samiha I Amer <<a href="mailto:lanre.durojaye@yahoo.com" class="">lanre.durojaye@yahoo.com</a>> wrote:<br class=""><br class="">Hello<br class=""><br class="">Please how do I prevent chimera from renumbering my protein residue sequence in cases where I am working with a pdb structure that does not cover all the residues?<br class=""><br class="">For example, the attached pdb protein file (5trf.pdb) was downloaded from the pdb database with the first 9 residues missing. The first residue in the pdb structure is the 10th residue and this was numbered accordingly but whenever I upload such structures on Chimera, the numbering of the residues tend to scatter because chimera automatically numbers the first residue in the structure as 1, just as it appeared in the attached mol2 file.<br class=""><br class="">Please how do I avoid/correct such<br class=""><br class="">Thank you.<br class=""><span id="cid:f1ccf2b7-b372-1503-a807-85fa2cf8afc0@yahoo.com"><5trf.pdb></span><span id="cid:144d2d8d-c33e-ae93-66b1-dec2a2f4db18@yahoo.com"><5TRF.mol2></span></blockquote></div></body></html>