<div class="__aliyun_email_body_block"><div  style="clear:both;"><span  style="font-family:Tahoma,Arial,STHeiti,SimSun;font-size:14.0px;color:#000000;">Dear all,</span></div><div  style="clear:both;"><span  style="font-family:Tahoma,Arial,STHeiti,SimSun;font-size:14.0px;color:#000000;"><br ></span></div><div  style="clear:both;"><span  style="font-family:Tahoma,Arial,STHeiti,SimSun;font-size:14.0px;color:#000000;">I am trying to learn preparing publishable figures of cryo-EM protein structure. I try to reproduce a figure (fig. 4A) from a paper published in Nature (https://doi.org/10.1038/s41586-020-2571-7).</span></div><div  style="clear:both;">Could you please find the original figure in the publication and the one I prepared in the following link？</div><div  style="clear:both;"><br ></div><div  style="clear:both;">https://drive.google.com/file/d/1dwQYT9-bGbs7OHi8e351SmE0FYr6FLK4/view?usp=sharing</div><div  style="clear:both;"><br ></div><div  style="clear:both;">The original figure (a) is for EMD-22156. I download the map for <span  style="color:#000000;font-family:Tahoma,Arial,STHeiti,SimSun;font-size:14.0px;font-style:normal;font-variant-ligatures:normal;font-variant-caps:normal;font-weight:400;text-align:start;text-indent:.0px;text-transform:none;widows:2;background-color:#ffffff;text-decoration-style:initial;text-decoration-color:initial;float:none;display:inline;">EMD-22156 form <a  href="https://www.ebi.ac.uk/pdbe/emdb/">https://www.ebi.ac.uk/pdbe/emdb/</a>, loaded it in Chimera, adjusted the range, hide the dusted, performed segmentation, and get images like (b). </span></div><div  style="clear:both;"><span  style="color:#000000;font-family:Tahoma,Arial,STHeiti,SimSun;font-size:14.0px;font-style:normal;font-variant-ligatures:normal;font-variant-caps:normal;font-weight:400;text-align:start;text-indent:.0px;text-transform:none;widows:2;background-color:#ffffff;text-decoration-style:initial;text-decoration-color:initial;float:none;display:inline;">The orignal figure (a) has plenty of structural detailed, and the helix can be clearly seen, but the images I prepared do not like that and look ugly. </span></div><div  style="clear:both;"><span  style="color:#000000;font-family:Tahoma,Arial,STHeiti,SimSun;font-size:14.0px;font-style:normal;font-variant-ligatures:normal;font-variant-caps:normal;font-weight:400;text-align:start;text-indent:.0px;text-transform:none;widows:2;background-color:#ffffff;text-decoration-style:initial;text-decoration-color:initial;float:none;display:inline;"><br ></span></div><div  style="clear:both;"><span  style="color:#000000;font-family:Tahoma,Arial,STHeiti,SimSun;font-size:14.0px;font-style:normal;font-variant-ligatures:normal;font-variant-caps:normal;font-weight:400;text-align:start;text-indent:.0px;text-transform:none;widows:2;background-color:#ffffff;text-decoration-style:initial;text-decoration-color:initial;float:none;display:inline;">Could you give me some detailed step-by-step instruction on how this original figure was prepared in chimera?</span></div><div  style="clear:both;"><span  style="color:#000000;font-family:Tahoma,Arial,STHeiti,SimSun;font-size:14.0px;font-style:normal;font-variant-ligatures:normal;font-variant-caps:normal;font-weight:400;text-align:start;text-indent:.0px;text-transform:none;widows:2;background-color:#ffffff;text-decoration-style:initial;text-decoration-color:initial;float:none;display:inline;"><br ></span></div><div  style="clear:both;"><span  style="color:#000000;font-family:Tahoma,Arial,STHeiti,SimSun;font-size:14.0px;font-style:normal;font-variant-ligatures:normal;font-variant-caps:normal;font-weight:400;text-align:start;text-indent:.0px;text-transform:none;widows:2;background-color:#ffffff;text-decoration-style:initial;text-decoration-color:initial;float:none;display:inline;">With many many thanks</span></div><div  style="clear:both;"><span  style="color:#000000;font-family:Tahoma,Arial,STHeiti,SimSun;font-size:14.0px;font-style:normal;font-variant-ligatures:normal;font-variant-caps:normal;font-weight:400;text-align:start;text-indent:.0px;text-transform:none;widows:2;background-color:#ffffff;text-decoration-style:initial;text-decoration-color:initial;float:none;display:inline;"><br ></span></div><div  style="clear:both;"><span  style="color:#000000;font-family:Tahoma,Arial,STHeiti,SimSun;font-size:14.0px;font-style:normal;font-variant-ligatures:normal;font-variant-caps:normal;font-weight:400;text-align:start;text-indent:.0px;text-transform:none;widows:2;background-color:#ffffff;text-decoration-style:initial;text-decoration-color:initial;float:none;display:inline;">Yeping Sun  </span></div></div>