<html><head><meta http-equiv="content-type" content="text/html; charset=utf-8"></head><body dir="auto"><div dir="ltr">Hi Chun-Nan,</div><div dir="ltr"><br></div><div dir="ltr"> Use our newer program ChimeraX. It has a new surface calculation code that never fails. Also try full or soft lighting in the toolbar to get much nicer depth perception than Chimera. Surface calculation often fails in Chimera for large structures especially on Windows.</div><div dir="ltr"><br></div><div dir="ltr"> Tom</div><div dir="ltr"><br><blockquote type="cite">On Apr 20, 2020, at 8:08 AM, Chun-Nan Chen <chunnan.chen@singlecelltechnology.com> wrote:<br><br></blockquote></div><blockquote type="cite"><div dir="ltr"><meta http-equiv="Content-Type" content="text/html; charset=us-ascii"><meta name="Generator" content="Microsoft Word 15 (filtered medium)"><style><!--
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</o:shapelayout></xml><![endif]--><div class="WordSection1"><p class="MsoNormal"><span style="font-size:12.0pt">Hello,<o:p></o:p></span></p><p class="MsoNormal"><span style="font-size:12.0pt"><o:p> </o:p></span></p><p class="MsoNormal"><span style="font-size:12.0pt">I am trying to visualize the SARS-CoV-2 spike protein, which is a trimer (PDB no. 6CRZ). I wish to surface chain B, which is in an “up” conformation) but it kept giving me an error message. I tried the “split” command and other solutions without success. I could surface just the B chain but I really want to surface all 3 chains and color the RBD.<o:p></o:p></span></p><p class="MsoNormal"><span style="font-size:12.0pt"><o:p> </o:p></span></p><p class="MsoNormal"><span style="font-size:12.0pt">Could anyone help me with this issue?<o:p></o:p></span></p><p class="MsoNormal"><span style="font-size:12.0pt"><o:p> </o:p></span></p><p class="MsoNormal"><span style="font-size:12.0pt">Thank you,<o:p></o:p></span></p><p class="MsoNormal"><span style="font-size:12.0pt"> <o:p></o:p></span></p><p class="MsoNormal"><span style="font-size:12.0pt">Chun-Nan Chen, Ph.D.<o:p></o:p></span></p><p class="MsoNormal"><span style="font-size:12.0pt">CEO<o:p></o:p></span></p><p class="MsoNormal"><span style="font-size:12.0pt">Single Cell Technology, Inc.<o:p></o:p></span></p><p class="MsoNormal"><span style="font-size:12.0pt">6280 San Ignacio Ave. Suite E<o:p></o:p></span></p><p class="MsoNormal"><span style="font-size:12.0pt">San Jose, CA 95119<o:p></o:p></span></p><p class="MsoNormal"><span style="font-size:12.0pt">Mobile: (408) 769-2171<o:p></o:p></span></p><p class="MsoNormal"><span style="font-size:12.0pt">Email: <a href="mailto:chunnan.chen@singlecelltechnology.com"><span style="color:#0563C1">chunnan.chen@singlecelltechnology.com</span></a><o:p></o:p></span></p><p class="MsoNormal"><span style="font-size:12.0pt">Web: <a href="http://www.singlecelltechnology.com/"><span style="color:#0563C1">singlecelltechnology.com</span></a></span><o:p></o:p></p><p class="MsoNormal"><o:p> </o:p></p></div><span>_______________________________________________</span><br><span>Chimera-users mailing list: Chimera-users@cgl.ucsf.edu</span><br><span>Manage subscription: http://plato.cgl.ucsf.edu/mailman/listinfo/chimera-users</span><br></div></blockquote></body></html>